CN102405939B - Nematicidal combination comprising paecilomyces lilacinus and carbosulfan - Google Patents

Nematicidal combination comprising paecilomyces lilacinus and carbosulfan Download PDF

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CN102405939B
CN102405939B CN 201110289518 CN201110289518A CN102405939B CN 102405939 B CN102405939 B CN 102405939B CN 201110289518 CN201110289518 CN 201110289518 CN 201110289518 A CN201110289518 A CN 201110289518A CN 102405939 B CN102405939 B CN 102405939B
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nematode
carbosulfan
paecilomyces lilacinus
root
composition
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CN102405939A (en
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孙新宇
王敏
刘伟
徐艳吉
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DEQIANG BIOLOGICAL Co Ltd
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DEQIANG BIOLOGICAL Co Ltd
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Abstract

The invention relates to nematicidal combination comprising paecilomyces lilacinus and carbosulfan. The combination comprises the paecilomyces lilacinus and the carbosulfan. The weight rate of the paecilomyces lilacinus to the carbosulfan is 100-1000 : 1-4, wherein the quantity of living bacterium of the paecilomyces lilacinus is 1-100 hundreds of millions cfu/g. The two pesticides in the combination of the invention are synergy in the prevention of the nematode, and the chemical pesticide and the microbial pesticide jointly act well. The invention realizes the action of simultaneously preventing the nematodes and the other pests by implementing the drug for one time. The combined use of the two pesticides greatly reduces the single use quantity of the pesticide.

Description

A kind of nematicidal composition that comprises Paecilomyces lilacinus and carbosulfan
Technical field
The invention belongs to agrochemical field, particularly relate to the nematicidal composition that comprises Paecilomyces lilacinus and carbosulfan.
Background technology
Nematode has another name called worm, belongs to invertebrate Nemathelminthes Nematoda, and the nematode hazard of plant symptom that causes that causes harm is taken as Disease Processing over a long time.Compare with pathogenic microorganisms such as fungi, bacterium, viruses, pathogenic nematode has initiatively trend and thrusts the host, and shifts the characteristics that cause harm voluntarily with lancet.The causing harm of nematode, be not only and draw plant nutrition and cause that the underproduction and quality descend, and the plant roots cell transition is increased becomes goiter and tumor, loses the ability that absorbs nutrient and moisture, and plant is declined extremely; In addition, causing harm of nematode, can cause also that plant is easier to suffer the attack of pathogen and cause crop that disease takes place.
Though the control of nematodosis has obtained bigger progress, also there is certain problem.The consciousness that mainly shows integrated control is thin, puts undue emphasis on the importance of chemical pesticide, has ignored the reasonable utilization of other prophylactico-therapeutic measures; Monocrop continuous cropping, cropping index height, or the unreasonable of chemical fertilizer use cause the trend that being of root knot nematode disease increase the weight of year by year, up to the present, form commercialization production and be widely used in the nematode biological prevention and control agent in land for growing field crops very few both at home and abroad.
For the chemical agent of preventing and treating root knot nematode disease fumigation type is arranged, as Celfume, chloropicrin and D-D mixture etc.Also have, as carbofuran, Aldicarb and Isofenphos methyl, a collection of chemical agents such as phonamiphos, Nemacur, gram line pellet, phoxim, Mi Leer and carbosulfan are processed into the nematocide type at present, and obtained good effect in the root knot nematode disease of crops such as peanut, tobacco, sugarcane, cucumber, traditional Chinese medicine aspect preventing and treating, at present, the nematocide of widespread usage also has the last ten thousand, metham-sodium, dazomet, lythidathion and dithiocyano-methane etc. both at home and abroad.
The bacterium that parasitizes root-knot nematode has Pasteurella,, puncture Pasteur bacillus, Pseudomonas fluorescence, Bacillus sphaericus, bacillus subtilis, bacillus thuringiensis,Bt, rhizosphere bacteria has preventive effect to Meloidogyne incognita, can reduce the root knot number, increase root system, it is heavy to increase root.
Paecilomyces lilacinus can effectively be prevented and treated root-knot nematode (Meloidogynespp.) and Cyst nematode (Heterodera sp.), to the ovum parasitic rate of Meloidogyne incognita up to 60%~70%.Paecilomyces lilacinus is prevented and treated usefulness to multiple nematode, and its host has root-knot nematode, Cyst nematode, golden nematode, golden nematode, is biological prevention and control agent (Amoncho etal, 1995 of the optimum future of control root-knot nematode; Angieri et al, 1997; Khan et al, 1988,1992,2000).Because this bacterium can adapt to different weather conditions, and line eggs is had higher parasitic rate, can effectively control soil nematodes quantity, obviously reduce harm, be widely used in the production practices.At present, the nematologist of nearly 70 countries in the world prevents and treats plant nematodes such as root-knot nematode, Cyst nematode with it, and has obtained preventive effect preferably.
In recent years, China researcher has also done a large amount of biological and ecological methods to prevent plant disease, pests, and erosion research to this bacterium, also confirms that this bacterium has higher parasitic rate to root-knot nematode, can effectively control the quantity of root-knot nematode in the soil, obviously reduces harm.Paecilomyces lilacinus has just reduced the illness that other pathogens of host plant cause to a certain extent to inhibition or the antagonism of microorganism on every side, better plays the biological and ecological methods to prevent plant disease, pests, and erosion effect.There is research to show that also this bacterium can also produce the metabolite of multiple biologically active.
Carbosulfan, english common name carbosulfan, chemical name: 2,3-dihydro-2, to be that carbofuran is low poison derivative for 2-dimethyl benzofuran-7-base (dibutylamino sulphur) methyl carbamate, carbosulfan, is the inhibitor of cholinesterase, have tag, stomach toxicity and systemic action, be used for the various pests such as aphid, mite, wireworm, colorado potato beetle, tealeaves cicada, oriental fruit moth, apple skin worm of crops such as control oranges and tangerines, fruit tree, cotton, paddy rice.Various nematodes had the good activity of killing.Insecticidal spectrum is wider, and aphid, citrus rust mite etc. is all had very high killing effect; Instant effect namely played a role after the dispenser in 20 minutes.But this agricultural chemicals exists too when killing nematode separately and uses consumption big, and effect is undesirable, and problems such as being easy to generate pesticide resistance is used in long-term single agent.
For realizing killing better various insects, the present invention is applied to crop control nematode and has obtained unforeseeable effect by carbosulfan and Paecilomyces lilacinus is composite.
Summary of the invention
For solving the problems of the technologies described above, the invention provides a kind of composition of preventing and treating nematode, said composition comprises Paecilomyces lilacinus bacterium and carbosulfan, and the weight ratio of Paecilomyces lilacinus and carbosulfan is 100-1000: 1-4, and wherein the viable bacteria amount that contains of Paecilomyces lilacinus is 1-100 hundred million cfu/ gram.
Wherein 1-100 hundred million cfu/ gram refers to that generally the bacterial number alive in every gram medicine is hundred million of 1-100, or perhaps the quantity of living spores is hundred million of 1-100.
In the above-mentioned composition, the weight ratio of preferred Paecilomyces lilacinus and carbosulfan is 200-800: 1-3.
In the above-mentioned composition, the viable bacteria amount that contains of preferred Paecilomyces lilacinus is 1-10 hundred million cfu/ gram, especially is preferably 500,000,000 cfu/ gram.
Above-mentioned nematicidal composition of the present invention can also contain other insecticide or nematocide, Acetamiprid for example, acrinathrin, Aldicarb, α-cypermethrin, aluminum phosphate, Amitraz, nimbin, azinphos-methyl, Benfuracard micro, β-cyfloxylate, β-cypermethrin, Biphenthrin, borax, Brodifacoum, fenisobromolate, Buprofezin, Buprofezin, cadusafos, carbaryl, Padan's hydrochloride, the bromine worm is clear, chlopyrifos, citronella oil, four mite piperazines, copper, coumatetralyl, cynock, cyfloxylate, plictran, cypermethrin, fly eradication amine, d_allethrin, dazomet, decis, demeton-methyl, diazinon, dichlorvos, dicofol, difenacoum, diflubenzuron, disulfoton, emaricin, 5a,6,9,9a-hexahydro-6,9-methano-2,4, sumicidin, phonamiphos, phonamiphos, ethylene dibromide, second mite azoles, fenamiphos, fenamiphos, fenazaquin, benzene fourth element, fenbutatin oxide, sumithion, fenoxycarb, fenpropathrin, azoles mite ester, fenthion, sumicidin, fluorine worm nitrile, flufenoxuron, flumethrin, lythidathion, Fumngillin, furfural, the garlic extract, hydramethylnon, Imidacloprid, indenes worm prestige, λ-cyhalothrin, the Acarus tritici urea, magnesium phosphide, mancozeb, the maple lactone, the malathion, the methaldehyde, metham-sodium, acephatemet, methidathion, methiocarb, Methomyl, Celfume, methyl-parathion, Menite, more visit rhzomorph, mineral oil, the fluorine uride, omethoate, neighbour-phenylphenol, oxamyl, metilomerkaptofosoksid, parathion, permethrin, phenthoate dimephenthoate cidial, thimet, phosmet, phoxim, Aphox, many sulfuration sulphur, fatty acid sylvite, Profenofos, alkynes mite spy, unden, protein hydrolysate, Toyodan, pyrethrin, the pyrrole propyl ether, quinalphos, rapeseed oil, rotenone, silica-based repellent agent, prodan, pleocidin, spiral shell mite ester, sulphur, tartar emetic, taufluvalinate, the worm hydrazides, Temefos, Terbufos, Ravap, the tetradecene yl acetate, tetradiphon, thiophene worm quinoline, thiophene worm piperazine, the two prestige of sulphur, the plug logical sequence, chlorophos, kill the bell urea, the spy lures ketone, cypermethrin, zinc phosphide etc., if possible contain a kind of in the above-mentioned insecticide incessantly, can contain more than one.The insecticide that adds other is generally the effect that strengthens composition kill pests of the present invention, on the other hand, also can strengthen the control efficiency of nematode.
Above-mentioned composition of the present invention is prepared into wetting powder, suspending agent, granule or microcapsule formulations.The method that the application above-mentioned composition prepares described preparation can be the common method preparation that those skilled in the art prepare preparation.
Above-mentioned composition of the present invention can be for line insect types such as the plant nematode class killed, and preferred, above-mentioned composition of the present invention can be used for killing tobacco root-knot nematode or paddy rice root-knot nematode.
Above-mentioned composition of the present invention can have for the crop that prevents and treats the nematodiasis invasion and attack: can be used for the nematoda control of vegetable crop fruit trees such as soybean, beet, potato, wheat class, corn, paddy rice, cotton, peanut, banana, citrus, pineapple, tea shoot, sugarcane, sweet potato, tobacco, red bayberry, Momordica grosvenori, kiwi fruit, peach, tomato, capsicum, eggplant, persimmon, carrot, grape, strawberry, fig, cucumber, celery, pumpkin, muskmelon, watermelon, balsam pear, Kidney bean, gymnospermous plants is as pine tree.
The present invention also provides above-mentioned composition to be used for the purposes of control nematode, and wherein said nematode is preferably tobacco root-knot nematode or paddy rice root-knot nematode.
Composition of the present invention can be used by root when nematoda control, and leaves and stems is used, and concrete application process such as liquid level spray, and root is mixed soil and sowed, and uses by filling root soluble in water etc.Amount of application is every mu of (667 square metres) 1-10kg, and preferred, amount of application is 1-5kg.
Preferably, the invention provides and use the method that above-mentioned composition is used for the control plant root-knot nematode, it is characterized in that composition mixed that soil is sowed or water-soluble filling root is applied to plant root soil, described crop is paddy rice or tobacco.
The beneficial effect of composition of the present invention is:
Composition of the present invention is developed for effectively preventing and treating nematode, can effectively prevent and treat nematode on the one hand, two kinds of mutual Synergistics of agricultural chemicals in the control of nematode, and chemical pesticide and microbial pesticide concur.In addition, because carbosulfan can also desinsection, killing when the nematode aspect strengthens the nematicide effect of Paecilomyces lilacinus, can also kill the insect of crop growth environment, realizing that the applied once medicine prevents and treats the effect of nematode and other insects simultaneously.The usage amount that greatly reduces independent agricultural chemicals is used in the mixing of two kinds of agricultural chemicals.
Embodiment
In order to understand the present invention, further specify the present invention with embodiment below, but do not limit the present invention.
Paecilomyces lilacinus among raw material sources: the embodiment 1 is 500,000,000 spore alive/g Paecilomyces lilacinus granules that Seoul Heilongjiang Qianger biochemical technology development Co., Ltd sells.The former medicine of carbosulfan is that the market purchase obtains.
Embodiment 1 Paecilomyces lilacinus and carbosulfan composition are to the preventive effect of paddy rice root-knot nematode
Use Paecilomyces lilacinus and carbosulfan and handle the paddy rice root-knot nematode, comprise that independent application prevents and treats the paddy rice root-knot nematode and control paddy rice root-knot nematode is used in two kinds of active ingredients mixing, application process is root soil to be irritated root use, the concentration of Paecilomyces lilacinus agricultural chemicals is 500,000,000 living spores/grams, application dosage is that the weight that every mu (667 square metres) are used is calculated, the carbosulfan application dosage is that the weight that every mu (667 square metres) are used is calculated, paddy rice root-knot nematode at identical nematodiasis carries out dispenser, measured preventive effect at 24 hours, repeated test 5 times is calculated by mean value.The value of preventive effect refers to that the percentage of killing nematode represents, according to the synergy situation of two kinds of medicaments of cobby formula clearing. specifically test retrieve data and see table.
Composition Amount of application/every mu Theoretical preventive effect Actual preventive effect The synergy situation
Paecilomyces lilacinus 1Kg - 53.69% -
Paecilomyces lilacinus 4Kg - 76.24% -
Carbosulfan 5g - 49.98% -
Carbosulfan 15g - 85.85% -
Paecilomyces lilacinus+carbosulfan 1Kg+5g 76.84% 84.68% Synergistic
Paecilomyces lilacinus+carbosulfan 1Kg+15g 93.45% 100.00% Synergistic
Paecilomyces lilacinus+carbosulfan 4Kg+5g 88.16% 98.45% Synergistic
Paecilomyces lilacinus+carbosulfan 4Kg+15g 96.64% 100.00% Synergistic
Find that by above-mentioned experiment Paecilomyces lilacinus is by mixing the independent preventive effect that has strengthened the paddy rice root-knot nematode with carbosulfan.Embody the effect of Synergistic for the control of paddy rice root-knot nematode.The paddy rice of having used composition is on average increased production 2-3% than using Paecilomyces lilacinus separately and using the carbosulfan volume increase separately.The insect of rice root is answered and reduces, and total eradicative rate of other insects has reached more than 82%.

Claims (1)

1. composition of preventing and treating nematode, said composition comprises Paecilomyces lilacinus bacterium and carbosulfan, and the weight ratio of Paecilomyces lilacinus and carbosulfan is 200-800:1-3, and wherein the viable bacteria amount that contains of Paecilomyces lilacinus is 500,000,000 cfu/ grams.
2. composition according to claim 1, said composition is prepared into wetting powder, suspending agent, granule or microcapsule formulations.
3. each composition of claim 1-2 is used for the purposes of control nematode, and wherein said nematode is tobacco root-knot nematode or paddy rice root-knot nematode.
4. each composition of claim 1-2 is used for the method for control plant root-knot nematode, it is characterized in that composition mixed that soil is sowed or water-soluble filling root is applied to plant root soil, and described crop is paddy rice or tobacco.
CN 201110289518 2011-09-27 2011-09-27 Nematicidal combination comprising paecilomyces lilacinus and carbosulfan Active CN102405939B (en)

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Publication number Priority date Publication date Assignee Title
CN102742607B (en) * 2012-07-23 2014-05-07 中国农业大学 Paecilomyces lilacinus fungal spore oil suspending agent and preparation method as well as application thereof
CN103789211B (en) * 2012-10-31 2015-09-30 国家海洋局第三海洋研究所 One strain has the abyssal sediment source Paecilomyces lilacinus A65 of anti-tealeaves pathomycete activity
ES2667555T3 (en) 2012-12-03 2018-05-11 Bayer Cropscience Ag Composition comprising a biological control agent and an insecticide
WO2014086756A1 (en) * 2012-12-03 2014-06-12 Bayer Cropscience Ag Composition comprising a biological control agent and an insecticide
CN104818216B (en) * 2015-01-20 2018-01-16 鲁东大学 One plant is used to prevent and treat tomato and the Paecilomyces lilacinus of grape root knot nematode disease evil
CN105875661A (en) * 2016-04-29 2016-08-24 山东胜伟园林科技有限公司 Biological pesticide containing Paecilomyces lilacinus for preventing and controlling soybean diseases and insect pests and preparation method thereof
CN106538606A (en) * 2016-10-15 2017-03-29 佛山市盈辉作物科学有限公司 A kind of nematicidal composition of fluorine-containing thiophene worm sulfone and Paecilomyces lilacinus
CN106942280A (en) * 2017-03-23 2017-07-14 佛山市普尔玛农化有限公司 Nematicidal composition containing tioxazafen and Paecilomyces lilacinus
CN111374129A (en) * 2020-03-23 2020-07-07 四川省兰月科技有限公司 Nematicide containing 1-octen-3-ol and preparation method and application thereof

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WO2011100424A1 (en) * 2010-02-12 2011-08-18 Bayer Croscience Lp Methods for reducing nematode damage to plants
CN102077846A (en) * 2011-01-21 2011-06-01 佛山市盈辉作物科学有限公司 Nematoid-killing composition containing abamectin and paecilomyces lilacinus

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