CN105875661A - Biological pesticide containing Paecilomyces lilacinus for preventing and controlling soybean diseases and insect pests and preparation method thereof - Google Patents

Biological pesticide containing Paecilomyces lilacinus for preventing and controlling soybean diseases and insect pests and preparation method thereof Download PDF

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CN105875661A
CN105875661A CN201610276096.3A CN201610276096A CN105875661A CN 105875661 A CN105875661 A CN 105875661A CN 201610276096 A CN201610276096 A CN 201610276096A CN 105875661 A CN105875661 A CN 105875661A
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paecilomyces lilacinus
insect pests
preventing
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王胜
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Shandong Sunway Garden Technology Co Ltd
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Shandong Sunway Garden Technology Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/22O-Aryl or S-Aryl esters thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N61/00Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30Microbial fungi; Substances produced thereby or obtained therefrom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/12Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Agronomy & Crop Science (AREA)
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Abstract

The invention provides a biological pesticide containing Paecilomyces lilacinus for preventing and controlling soybean diseases and insect pests and a preparation method thereof. The biological pesticide is prepared from, by weight, 20-28 parts of carbofuran, 1.8-3 parts of Paecilomyces lilacinus, 2.4-3.5 parts of notoginseng extract, 2.3-3.4 parts of Siberian cocklebur powder, 1.5-2.2 parts of iron and sodium salt, 3.8-5.6 parts of a dispersant, 1.5-2.5 parts of a wetting agent, 2.3-3.5 parts of a dispersing agent, 8-12 parts of corn starch and 27-40 parts of water. The invention also provides application of the pesticide in saline-alkali land for soybean planting. The biological pesticide has the advantages that the prepared pesticide has the good preventing and controlling effect on the insect pests in soybean planting land, and the preventing effects on common insect pests in the soybean planting land, namely wireworms, mole crickets, grubs and cutworms, respectively reach 82.1-86.8%, 95.9-99.8%, 90.7-94.9% and 81.9-86.5%.

Description

A kind of containing Paecilomyces lilacinus for prevent and treat soybean diseases and insect pests biological insecticides and Preparation method
Technical field
The present invention relates to a kind of containing Paecilomyces lilacinus for preventing and treating biological insecticides and the preparation side thereof of soybean diseases and insect pests Method, belongs to technical field of pesticide.
Background technology
Paecilomyces lilacinus ((Paecilomyces lilacinus(Thorn.) Samson) belongs to endoparasitism fungus, is The Important Natural Enemy of some plant nematodes, it is possible to parasitize ovum, also can infect larva and female worm, can substantially alleviate various crop The harm of the nemases such as root-knot nematode, Cyst nematode, Ditylenchus dipsaci.
Paecilomyces lilacinus is effective bacterial parasite of root-knot nematode and white Cyst nematode ovum.Multiple nematicide is had preventing and treating effect Can, its host has root-knot nematode, Cyst nematode, golden nematicide, golden nematode, even people and animals' bellyworm, is to prevent and treat root knot line The most promising biological prevention and control agent of worm.
After the mould spore germination of pale purple blue or green plan, produced mycelia can penetrate the chorion of nematicide, larva and female insect body wall, Mycelia, in its internal absorption nutrition, breeds, and destroys ovum, larva and the normal physiological metabolism of female insect, thus causes planting Thing parasitic nematode is dead.
In the incubation of Paecilomyces varioti, particularly during special culture medium with Submerged fermentation, this bacterium can produce Raw abundant derivant, the first is similar to heteroauxing product, promotes plant roots when its most significant physiological function is low concentration System and the growth of plant, therefore execute bacterium at root system of plant and can not only substantially suppress nematode infection, and can promote plant nutrition device The growth of official, the sprouting to seed simultaneously also has facilitation with growth.
Paecilomyces lilacinus is after Paecilomyces lilacinus contacts with nematicide egg capsule to the inhibiting mechanism of root-knot nematode, at adherent base In matter, biocontrol microorganisms mycelia surrounds whole ovum, and mycelia end is thicker, owing to the activity of exogenous metabolism thing and mycosin enzyme makes Chorion top layer ruptures, and fungus invades and replaces subsequently.Also can excrete poison and nematicide is played toxic action.
Crops are all had a certain degree of Side effect by existing chemical insecticide, can cause soil in various degree The environmental pollutions such as ground, water source, chemical insecticide improper use can cause crops to produce poisoning, causes crop growth abnormal, Suppress its photosynthesis, affect crop yield, and second stubble crop be will also result in certain murder by poisoning.
Existing chemical insecticide, is divided into liquid and solid preparation two types, liquid preparation dispersible oil-suspending agent, Cream, aqueous emulsion, microemulsion;Solid preparation has wettable powder, soluble powder, water dispersible granules.
The defect that existing chemical insecticide exists is as follows:
(1) existing insecticide, just for the insect pest in planting site, not notable or the most anti-to the diseases control in planting site Effect, and existing insecticide is the most obvious to certain insect insecticidal effect;
(2), when existing insecticide is applied to Semen sojae atricolor pest control, Semen sojae atricolor is had certain side effect, poor stability, it is grown There is certain inhibitory action, and then reduce crop yield.
(3) after existing insecticide has been used, easily causing insecticide toxin residual, the quality of Semen sojae atricolor produces impact, Affect the edible safety of Semen sojae atricolor.
Summary of the invention
The present invention solves the deficiency that prior art exists, it is provided that a kind of Paecilomyces lilacinus that contains is used for preventing and treating Semen sojae atricolor disease pest Biological insecticides of evil and preparation method thereof, to realize following goal of the invention:
(1) insecticide prepared by the present invention is good to the prevention effect of the insect in soybean planting ground, to common in soybean planting ground Insect: wireworm, Gryllotalpa, Holotrichia diomphalia Bates, the preventive effect of cutworm significantly improve;
(2) while insecticide prepared by the present invention insect in carrying out soybean planting ground, the disease control to soybean plant strain There is effect equally;
(3), during insecticide prepared by the present invention is applied to salt-soda soil Semen sojae atricolor during pest control, Semen sojae atricolor is had no side effect, safety High.
For solving above technical problem, the technical solution used in the present invention is as follows:
A kind of containing Paecilomyces lilacinus for preventing and treating the biological insecticides of soybean diseases and insect pests, described insecticide, including carbofuran, light Purple Paecilomyces varioti, Radix Notoginseng extract, Herba Xanthii powder, ferrum sodium salt, dispersant.
The following is and the further of technique scheme is improved:
Described insecticide, the mass parts content of each raw material components is: carbofuran 20-28 part, Paecilomyces lilacinus 1.8-3 part, Radix Notoginseng Extract 2.4-3.5 part, Herba Xanthii powder 2.3-3.4 part, ferrum sodium salt 1.5-2.2 part, dispersant 3.8-5.6 part, wetting agent 1.5-2.3 Part, diffusant 2.5-3.5 part, corn starch 8-12 part, water 27-40 part.
Described wetting agent is sodium alkyl benzene sulfonate;Described dispersant is polycarboxylic acids sodium.
Described Herba Xanthii powder contains xanthostrumarin, resin, and xanthostrumarin content is 1.2%, resin content is 3.3%.
Described diffusant is sodium metnylene bis-naphthalene sulfonate.
Described Radix Notoginseng extract contains ginsenoside Rg1, ginsenoside Rb1, ginsenoside Rd, arasaponin R1, Radix Ginseng Saponin Re, the content of ginsenoside Rg1 is 32%, the content of ginsenoside Rb1 is 37%, the content of ginsenoside Rd is 9%, three The content of seven saponin R1 is 8%, the content of ginsenoside Re is 5%.
A kind of containing Paecilomyces lilacinus for the biological insecticides preventing and treating soybean diseases and insect pests and preparation method thereof, described preparation Method includes the preparation of Radix Notoginseng extract, and the preparation of Radix Notoginseng extract includes reflux, extract, step, precipitate with ethanol step and absorb-elute Step.
Described reflux, extract, step, is broken for 10 purpose fresh Radix Notoginseng granule fresh rhizomes of Panax notoginseng, adds the ethanol of 6 times amount 72%, Reflux, extract, 3 times, each 2h, add 0.1ml/200g cellulase, 0.2ml/200g pectase, available fresh Radix Notoginseng is extracted Liquid.
Described absorb-elute step, the Radix Notoginseng extracting solution that will prepare after precipitate with ethanol step, upper D101 macroporous resin column purification, on First quickly rush post after post with distilled water, the ethanol of 70% is eluant, and speed during eluting is 60/min, obtains Radix Notoginseng after eluting Extract.
A kind of containing Paecilomyces lilacinus for preventing and treating the biological insecticides of soybean diseases and insect pests and at salt-soda soil soybean planting In application, the consumption of described insecticide is 210~320g/100m2
Use above technical scheme, the invention have the benefit that
(1) insecticide prepared by the present invention is good to the prevention effect of the insect in soybean planting ground, to common in soybean planting ground Insect: wireworm, Gryllotalpa, Holotrichia diomphalia Bates, the preventive effect of cutworm respectively reach 82.1-86.8%, 95.9-99.8%, 90.7-94.9%, 81.9-86.5%。
(2) while insecticide prepared by the present invention insect in carrying out soybean planting ground, the disease to soybean plant strain Preventing and treating has effect equally.Disease to common in soybean planting ground: root rot, Semen sojae atricolor damping off, soybean cyst nematode Heterodera glycines The sick preventive effect of disease, Semen sojae atricolor damping-off, root-knot nematode respectively reach 72.6-78.9%, 70.8-75.6%, 94.2-98.6%, 75.4-80.3%、94.7-98.9%。
(3) insecticide that prepared by the present invention is without any side effects to soybean plant strain, and safety is high, also obtains beyond thought Effect, the effect of being significantly improved of the yield of soybean plant strain, per mu yield reaches 330-380 jin;Protein and carbohydrate in Semen sojae atricolor Content also significantly improves, and protein content is 32-38g/100g, and carbohydrate content is 21-29g/100g.
(4) described Paecilomyces lilacinus after death can decompose at host insects, will not produce insecticide toxin residual.
Detailed description of the invention
Hereinafter the preferred embodiments of the present invention are illustrated, it will be appreciated that preferred embodiment described herein is only used In the description and interpretation present invention, it is not intended to limit the present invention.
Embodiment 1 one kinds is used for preventing and treating the biological insecticides of soybean diseases and insect pests containing Paecilomyces lilacinus
Described insecticide, the mass ratio of each raw material components is:
Carbofuran 20 parts, Paecilomyces lilacinus 1.8 parts, Radix Notoginseng extract 2.4 parts, 2.3 parts of Herba Xanthii powder, ferrum sodium salt 1.5 parts, dispersant 3.8 parts, wetting agent 1.5 parts, diffusant 2.5 parts, corn starch 8 parts, 27 parts of water.
Described carbofuran, the weight/mass percentage composition of effective ingredient is 20%;
Described Paecilomyces lilacinus, the mass content of effective ingredient be 2,000,000,000 spores/gram;
Described Radix Notoginseng extract contains ginsenoside Rg1, ginsenoside Rb1, ginsenoside Rd, arasaponin R1, ginsenoside Re, the content of ginsenoside Rg1 is 32%, the content of ginsenoside Rb1 is 37%, the content of ginsenoside Rd is 9%, Radix Notoginseng soap The content of glycosides R1 is 8%, the content of ginsenoside Re is 5%;
Described Herba Xanthii powder contains xanthostrumarin, resin, and xanthostrumarin content is 1.2%, resin content is 3.3%;
Described ferrum sodium salt, the weight/mass percentage composition of effective ingredient is 90%;
Described wetting agent is sodium alkyl benzene sulfonate;
Described dispersant is polycarboxylic acids sodium;
Described diffusant is sodium metnylene bis-naphthalene sulfonate.
Embodiment 2 one kinds is used for preventing and treating the biological insecticides of soybean diseases and insect pests containing Paecilomyces lilacinus
Described insecticide, the mass ratio of each raw material components is:
Carbofuran 25 parts, Paecilomyces lilacinus 2.5 parts, Radix Notoginseng extract 3 parts, 3 parts of Herba Xanthii powder, ferrum sodium salt 2 parts, dispersant 5 parts, profit Humectant 2 parts, diffusant 3 parts, corn starch 10 parts, 35 parts of water.
Described carbofuran, the weight/mass percentage composition of effective ingredient is 20%;
Described Paecilomyces lilacinus, the mass content of effective ingredient be 2,000,000,000 spores/gram;
Described Radix Notoginseng extract contains ginsenoside Rg1, ginsenoside Rb1, ginsenoside Rd, arasaponin R1, ginsenoside Re, the content of ginsenoside Rg1 is 32%, the content of ginsenoside Rb1 is 37%, the content of ginsenoside Rd is 9%, Radix Notoginseng soap The content of glycosides R1 is 8%, the content of ginsenoside Re is 5%;
Described Herba Xanthii powder contains xanthostrumarin, resin, and xanthostrumarin content is 1.2%, resin content is 3.3%;
Described ferrum sodium salt, the weight/mass percentage composition of effective ingredient is 90%;
Described wetting agent is sodium alkyl benzene sulfonate;
Described dispersant is polycarboxylic acids sodium;
Described diffusant is sodium metnylene bis-naphthalene sulfonate.
Embodiment 3 one kinds is used for preventing and treating the biological insecticides of soybean diseases and insect pests containing Paecilomyces lilacinus
Described insecticide, the mass ratio of each raw material components is:
Carbofuran 28 parts, Paecilomyces lilacinus 3 parts, Radix Notoginseng extract 3.5 parts, 3.4 parts of Herba Xanthii powder, ferrum sodium salt 2.2 parts, dispersant 5.6 parts, wetting agent 2.3 parts, diffusant 3.5 parts, corn starch 12 parts, 40 parts of water.
Described carbofuran, the weight/mass percentage composition of effective ingredient is 20%;
Described Paecilomyces lilacinus, the mass content of effective ingredient be 2,000,000,000 spores/gram;
Described Radix Notoginseng extract contains ginsenoside Rg1, ginsenoside Rb1, ginsenoside Rd, arasaponin R1, ginsenoside Re, the content of ginsenoside Rg1 is 32%, the content of ginsenoside Rb1 is 37%, the content of ginsenoside Rd is 9%, Radix Notoginseng soap The content of glycosides R1 is 8%, the content of ginsenoside Re is 5%;
Described Herba Xanthii powder contains xanthostrumarin, resin, and xanthostrumarin content is 1.2%, resin content is 3.3%;
Described ferrum sodium salt, the weight/mass percentage composition of effective ingredient is 90%;
Described wetting agent is sodium alkyl benzene sulfonate;
Described dispersant is polycarboxylic acids sodium;
Described diffusant is sodium metnylene bis-naphthalene sulfonate.
Paecilomyces lilacinus used by above example 1 to embodiment 3 is buied by existing product on market.
Embodiment 4 one kinds is used for biological insecticides preventing and treating soybean diseases and insect pests and preparation method thereof containing Paecilomyces lilacinus
Described vegetable insecticide, dosage form is water dispersible granules, and preparation method includes:
(1) preparation of Radix Notoginseng extract:
A, reflux, extract,
Fresh rhizomes of Panax notoginseng is broken for 10 purpose fresh Radix Notoginseng granule, adds the ethanol of 6 times amount 72%, reflux, extract, 3 times, each 2h, then Add 0.1ml/200g cellulase, 0.2ml/200g pectase, available fresh Radix Notoginseng extracting solution;
B, precipitate with ethanol
By the fresh Radix Notoginseng extracting solution of said extracted, precipitate with ethanol 1 time, the time is 12h, and alcohol precipitation concentration is the ethanol of 70%, alcohol precipitation concentration with Sample liquid proportional is 1: 1;
C, absorption, eluting
By the Radix Notoginseng extracting solution after above-mentioned precipitate with ethanol, upper D101 macroporous resin column purification, after upper prop, first quickly rush post with distilled water, The ethanol of 70% is eluant, and the speed (dripping speed) during eluting is 60/min, obtains Radix Notoginseng extract after eluting.
(2) weigh
According to the insecticidal formulation described in embodiment 1-3, weigh each composition.
(3) mixing
Carbofuran, Paecilomyces lilacinus, Radix Notoginseng extract, Herba Xanthii powder, ferrum sodium salt, dispersant, wetting agent, diffusant and Semen Maydis are formed sediment Powder additions etc. put in blender and fully mix;
(4) pulverize
Solid material after above-mentioned mixing is put in jet mill, is crushed to suitable fineness through jet mill and divides Cloth;
(5) mediate
Putting in kneader by above-mentioned crushing rear material, uniformly add water in kneader kneading;
(6) pelletize, be dried
Above-mentioned kneaded wet stock is put into extruding pelletization in comminutor, the wet granular of extrusion is gone in fluid bed, Being dried under the airflow function of proper temperature, in product, residual moisture content controls to be advisable in 0.5~1.0%, prepares insecticide.
Embodiment 5 one kinds is used for preventing and treating the biological insecticides of soybean diseases and insect pests in soybean planting ground containing Paecilomyces lilacinus Application
Four experimental plots, respectively experimental plot A, experimental plot B, experimental plot C and examination is hewed out in soybean planting ground Testing community D, the length of each experimental plot is about 10-15m, and the consumption of insecticide is 210~320g/100m2
Experimental plot A: product in embodiment 1 is watered dilution by 600 times, the soybean plant strain in the A of experimental plot is carried out Pouring root, in Semen sojae atricolor seedling stage, go out a phase and the fruiting period of blooming respectively sprays 2 medicines, spray date is separated by 5-8 days, is sprayed onto blade and starts Drippage is degree.
Experimental plot B: product in embodiment 2 is watered dilution by 600 times, the soybean plant strain in the B of experimental plot is carried out Pouring root, in Semen sojae atricolor seedling stage, go out a phase and the fruiting period of blooming respectively sprays 2 medicines, spray date is separated by 5-8 days, is sprayed onto blade and starts Drippage is degree.
Experimental plot C: product in embodiment 3 is watered dilution by 600 times, the soybean plant strain in the C of experimental plot is carried out Pouring root, in Semen sojae atricolor seedling stage, go out a phase and the fruiting period of blooming respectively sprays 2 medicines, spray date is separated by 5-8 days,.
Experimental plot D: as controlled trial community, does not spray this insecticide, replaces insecticide to experimental plot D with clear water In soybean plant strain carry out pouring root.
Investigation and statistical method: within 15 days after fruiting period dispenser of blooming, investigating, 100 strain Semen sojae atricolor are investigated in each experimental plot, Add up killed strain number, check prevention effect.
Result of the test:
(1) insecticide prepared by the present invention is good to the prevention effect of the insect in soybean planting ground, to common in soybean planting ground Insect: wireworm, Gryllotalpa, Holotrichia diomphalia Bates, the preventive effect of cutworm respectively reach 82.1-86.8%, 95.9-99.8%, 90.7-94.9%, 81.9-86.5%, specific targets are shown in Table 1.
Insecticide prepared by table 1 present invention prevention effect to the insect in soybean planting ground
(2) while insecticide prepared by the present invention insect in carrying out soybean planting ground, the disease control to soybean plant strain There is effect equally.
Disease to common in soybean planting ground: root rot, Semen sojae atricolor damping off, soybean cyst nematode, Semen sojae atricolor stand The sick preventive effect of rot, root-knot nematode respectively reach 72.6-78.9%, 70.8-75.6%, 94.2-98.6%, 75.4-80.3%, 94.7-98.9%, specific targets are shown in Table 2.
Insecticide prepared by table 2 present invention prevention effect to the disease in soybean planting ground
(3) insecticide that prepared by the present invention is without any side effects to soybean plant strain, and safety is high, also obtains beyond thought effect Really, the effect of being significantly improved of the yield of soybean plant strain, per mu yield reaches 330-380 jin;In Semen sojae atricolor, protein and carbohydrate contain Amount also significantly improves, and protein content is 32-38g/100g, and carbohydrate content is 21-29g/100g, is specifically shown in Table 3.
Protein and dietary fiber content in the yield of table 3 Semen sojae atricolor, Semen sojae atricolor
Comparison: do not use insecticide of the present invention, replaces insecticide to spray with clear water.
Finally it is noted that the foregoing is only the preferred embodiments of the present invention, it is not limited to the present invention, Although being described in detail the present invention with reference to previous embodiment, for a person skilled in the art, it still may be used So that the technical scheme described in foregoing embodiments to be modified, or wherein portion of techniques feature is carried out equivalent. All within the spirit and principles in the present invention, any modification, equivalent substitution and improvement etc. made, should be included in the present invention's Within protection domain.

Claims (10)

1. one kind contains Paecilomyces lilacinus for preventing and treating the biological insecticides of soybean diseases and insect pests, it is characterised in that: described insecticide, Including carbofuran, Paecilomyces lilacinus, Radix Notoginseng extract, Herba Xanthii powder, ferrum sodium salt, dispersant.
The most according to claim 1 a kind of containing Paecilomyces lilacinus for preventing and treating the biological insecticides of soybean diseases and insect pests, its Being characterised by: described insecticide, the mass parts content of each raw material components is: carbofuran 20-28 part, Paecilomyces lilacinus 1.8-3 part, Radix Notoginseng extract 2.4-3.5 part, Herba Xanthii powder 2.3-3.4 part, ferrum sodium salt 1.5-2.2 part, dispersant 3.8-5.6 part, wetting agent 1.5-2.3 part, diffusant 2.5-3.5 part, corn starch 8-12 part, water 27-40 part.
The most according to claim 2 a kind of containing Paecilomyces lilacinus for preventing and treating the biological insecticides of soybean diseases and insect pests, its It is characterised by: described wetting agent is sodium alkyl benzene sulfonate;Described dispersant is polycarboxylic acids sodium.
The most according to claim 2 a kind of containing Paecilomyces lilacinus for preventing and treating the biological insecticides of soybean diseases and insect pests, its Being characterised by: described Herba Xanthii powder contains xanthostrumarin, resin, xanthostrumarin content is 1.2%, resin content is 3.3%.
The most according to claim 2 a kind of containing Paecilomyces lilacinus for preventing and treating the biological insecticides of soybean diseases and insect pests, its It is characterised by: described diffusant is sodium metnylene bis-naphthalene sulfonate.
The most according to claim 2 a kind of containing Paecilomyces lilacinus for preventing and treating the biological insecticides of soybean diseases and insect pests, its It is characterised by: described Radix Notoginseng extract contains ginsenoside Rg1, ginsenoside Rb1, ginsenoside Rd, arasaponin R1, Radix Ginseng Saponin Re, the content of ginsenoside Rg1 is 32%, the content of ginsenoside Rb1 is 37%, the content of ginsenoside Rd is 9%, three The content of seven saponin R1 is 8%, the content of ginsenoside Re is 5%.
7. one kind is used for biological insecticides preventing and treating soybean diseases and insect pests and preparation method thereof containing Paecilomyces lilacinus, and its feature exists In: described preparation method includes the preparation of Radix Notoginseng extract, and the preparation of Radix Notoginseng extract includes reflux, extract, step, precipitate with ethanol step And absorb-elute step.
The most according to claim 7 a kind of containing Paecilomyces lilacinus for prevent and treat soybean diseases and insect pests biological insecticides and Preparation method, it is characterised in that: described reflux, extract, step, fresh rhizomes of Panax notoginseng is broken for 10 purpose fresh Radix Notoginseng granule, adds 6 times The ethanol of amount 72%, reflux, extract, 3 times, each 2h, add 0.1ml/200g cellulase, 0.2ml/200g pectase, can obtain To fresh Radix Notoginseng extracting solution.
The most according to claim 7 a kind of containing Paecilomyces lilacinus for prevent and treat soybean diseases and insect pests biological insecticides and Preparation method, it is characterised in that: described absorb-elute step, the Radix Notoginseng extracting solution that will prepare after precipitate with ethanol step, upper D101 macropore Resin column purification, first quickly rushes post with distilled water after upper prop, the ethanol of 70% is eluant, and speed during eluting is 60/min, Radix Notoginseng extract is obtained after eluting.
10. one kind is used for preventing and treating the biological insecticides of soybean diseases and insect pests and in the soybean planting of salt-soda soil containing Paecilomyces lilacinus Application, it is characterised in that: the consumption of described insecticide is 210~320g/100m2
CN201610276096.3A 2016-04-29 2016-04-29 Biological pesticide containing Paecilomyces lilacinus for preventing and controlling soybean diseases and insect pests and preparation method thereof Pending CN105875661A (en)

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CN101803620A (en) * 2010-03-15 2010-08-18 潍坊奥丰作物病毒防治技术服务有限公司 Plant active glycoside peptide fungicide and preparation method thereof
CN102405939A (en) * 2011-09-27 2012-04-11 德强生物股份有限公司 Nematicidal combination comprising paecilomyces lilacinus and carbosulfan
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