CN109511407A - A kind of method of radish drought tolerance Rapid identification - Google Patents
A kind of method of radish drought tolerance Rapid identification Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
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Abstract
The invention belongs to plant resistance to environment stress identification technology fields, and in particular to a kind of method of radish drought tolerance Rapid identification.Radish seed is divided into A group and B group by this method, it is wherein the plantation of A group in arid soil, B group is planted in normal moisture content soil, mass loss situation after measuring the germination percentage, seedling percent, drying process of A group and B group respectively, building formula calculate Drought tolerance index: F=(Y1/Y2+ (WB1-WB2)/(WA1-WA2)+M1/M2) × 10.The present invention a kind of dedicated breeding for drought resistance method of radish newly developed, for the growth characteristics of the fleshy root of radish, the accuracy rate of qualification result is can be improved as judge index in the mass loss situation after germination percentage, seedling percent and drying process；Identification of Drought can be completed in this method before Seedling Stage, time-consuming short.
Description
Technical field
The invention belongs to plant resistance to environment stress identification technology fields, and in particular to a kind of method of radish drought tolerance Rapid identification.
Background technique
China's arid soil is more, and arid directly affects crop yield, therefore the screening step of drought-enduring variety very must
It wants.Since different crops growth phenotype is different, some edible Aerial fruits, some edible Subterranean fruits, different crops
The method that Resistance Identification uses is also less identical.
For example " drought tolerance of soybean germplasm is identified and drought-enduring index screening, Wang Wei, Soybean Science, the 5th phase of volume 34 " is public
The drought tolerance identification method for having opened a kind of soybean germplasm, by observation soybean plant strain under water shortage environment root length, surface area,
The parameters such as nitrogen fixing capacity judge the drought tolerance of soybean." research of Drought Resistance in Maize identification method, Fu Fengling, Sichuan Agricultural University,
Master thesis " by plant height, interval time male and female flowering phase, go out the indexs such as liquid speed degree, root weight, proline content and identify jade
The drought tolerance of rice plant.Above-mentioned two documents are all to have filtered out to grow closely related index with the plant, and then accurate
Judge drought resistance in plants.
Radish is common vegetable crop, and screening drought tolerance radish germplasm is the important hand of radish breeder
Section.But the prior art lacks the drought tolerance identification method for being directed to radish.Common seed water stress experiment can only judge trailing plants
Foretell the germination of seed, only judges that drought tolerance has one-sidedness, qualification result inaccuracy by germination percentage.If each product
Kind all cultures are then needed to the radish stage of ripeness then by multiple indexs such as germination percentage, fruit weight come qualification result out again
Long time could complete the work of kind Identification of Drought.Therefore needing to develop one kind can accurate judgement radish drought resistance and consumption
When shorter new method.
Summary of the invention
Drought resistance mirror can be completed in a kind of method of radish drought tolerance Rapid identification provided by the invention before Seedling Stage
It is fixed, using the mass loss situation after germination percentage, seedling percent and drying process as judge index, qualification result can be improved
Accuracy rate.
A kind of method for being to provide radish drought tolerance Rapid identification of the invention, comprising the following steps: the following steps are included:
Radish seed is divided into A group and B group by S1, wherein being the plantation of A group in arid soil, B group is planted in normal aqueous
It measures in soil；
S2 measures the germination percentage Y2 of germination percentage Y1 and the B group of A group respectively, and the criterion of germination is: 2 cotyledons are grown,
And cotyledon is emerged 5~10mm of soil；
S3, field management count the seedling percent of A group when radish growth breaks tripe to 5~8 true leaf, that is, fleshy roots
Seedling percent M2, the A group and B component of M1 and B group do not extract a part of whole seedlings, spare；
S4 measures A group seedling average quality WA1, measures B group seedling average quality WB1, then by A group seedling, B group children
Seedling is placed under 30~45 DEG C of ventilated environments and is dried 4h, measures A group seedling average quality WA2 again, and measurement B group seedling is flat
Equal quality WB2；
S5 calculates Drought tolerance index according to following formula:
F=(Y1/Y2+ (WB1-WB2)/(WA1-WA2)+M1/M2) × 10；
If A group germination percentage is 0, the value of (WA1-WA2)/(WB1-WB2) is denoted as 0, no longer needs to the step of carrying out S4；
If F >=25, which is that height is drought-enduring；If 18≤F < 25, the kind is generally drought-enduring, should if F < 18
Kind is not drought-enduring.
Preferably, the method for above-mentioned radish drought tolerance Rapid identification, the water content of arid soil are 10~20%, are normally contained
The water content of water soil is 30~70%.
Preferably, the quantity of the method for above-mentioned radish drought tolerance Rapid identification, A group and B group radish is 200~300.
Preferably, the method for above-mentioned radish drought tolerance Rapid identification, the measuring method of germination percentage are as follows: with 100 seeds
For calculating basis C1, germinable seed amount is denoted as C2, germination percentage=C2/C1 after plantation.
Preferably, the method for above-mentioned radish drought tolerance Rapid identification, the calculation method of seedling percent are as follows: seedling survives
Grow up to strain number total amount/germination strain number total amount of seedling in rate=germination strain.
Preferably, the method for above-mentioned radish drought tolerance Rapid identification, the calculation method of seedling average quality are as follows: growing up to
10 plants are randomly selected in the plant of seedling as experimental subjects, are weighed respectively, are taken the average value of 10 plants of seedling qualities as drying
Seedling average quality before processing, after being dried, still weighs respectively, takes the average value of 10 plants of seedling qualities as dry
Dry treated seedling average quality.
Preferably, the method for above-mentioned radish drought tolerance Rapid identification, arid soil and normal moisture content soil are separated by spacing
It is 30~50 meters, the water content of soil is maintained by way of manual priming.
Preferably, the method for above-mentioned radish drought tolerance Rapid identification, radish are planted by the way of digging cave dibbling, every cave one
Grain seed, 5~15cm of spacing in the rows, 10~20cm of line-spacing.
Compared with prior art, the method for radish drought tolerance Rapid identification of the invention, has the advantages that
The present invention a kind of dedicated breeding for drought resistance method of radish newly developed, the growth for the fleshy root of radish are special
Property, the standard of qualification result can be improved as judge index in the mass loss situation after germination percentage, seedling percent and drying process
True rate.
If merely with germination percentage as Resistance Identification index, we by comparing embodiment 2 and embodiment 4 data
It is found that germination percentage, seedling percent differ larger in arid soil for same kind, main cause is germination
Also have with seeds self water content compared with Important Relations, needed nutrient matter of germinateing is mainly derived from seed；It is special but when growth of seedling
It is not that fleshy root breaks the tripe phase, needs photosynthesis and absorption organic substance and large quantity of moisture, seed itself are aqueous from soil
Needed for amount is not able to satisfy completely, and from the time for being spaced 7~14 days of germinateing to 5~8 leaf periods, long-time water shortage leads to children
Seedling can not grow up to.So radish seed used in embodiment 4 have passed through immersion treatment, seed water content itself is higher, therefore it germinates
Rate is higher, but later period seedling percent is not high.Therefore, if single using germination percentage as drought resisting standard of perfection, standard can not be obtained
True result.
Identification of Drought can be completed in method of the invention before Seedling Stage, needs not wait for the radish maturity period, time-consuming
It is short, it is a kind of quick identification method, large-scale promotion is suitble to use.
Specific embodiment
The present invention is described in detail combined with specific embodiments below, but should not be construed as limitation of the invention.It is following
The test method of actual conditions is not specified in embodiment, operates usually according to normal condition, due to not being related to inventive point, thus it is not right
Its step is described in detail.
Embodiment 1
A kind of method of radish drought tolerance Rapid identification, No. 2 radish of Fuling red heart (known general drought tolerance radish product
Kind), comprising the following steps:
Radish seed is divided into A group and B group by S1, and the quantity of A group and B group radish is 300, wherein for the plantation of A group in
In arid soil, B group is planted in normal moisture content soil；The water content of arid soil is 10~20% (mass fractions), just
The water content of normal water content soil is 40~50% (mass fractions)；It is 30 that arid soil and normal moisture content soil, which are separated by spacing,
Rice maintains the water content of normal soil and arid soil by way of manual priming；
S2 measures the germination percentage Y2 of germination percentage Y1 and the B group of A group respectively, and the criterion of germination is: 2 cotyledons are grown,
And cotyledon is emerged 5~10mm of soil；
The measuring method of germination percentage is as follows: using 100 seeds as calculating basis C1, germinable seed amount note after plantation
For C2, germination percentage=C2/C1；
S3, field management, when radish growth to 6 true leaf, that is, fleshy roots breaks tripe, count the seedling percent M1 of A group with
Seedling percent M2, the A group and B component of B group do not extract a part of whole seedlings, spare；
Grow up to strain number total amount/germination strain number total amount of seedling in seedling percent=germination strain；
S4 measures A group seedling average quality WA1, measures B group seedling average quality WB1, then by A group seedling, B group children
Seedling is placed under 35 DEG C of ventilated environments and is dried 4h, measures A group seedling average quality WA2 again, measures B group seedling and is averaged matter
Measure WB2；
The calculation method of seedling average quality is as follows: 10 plants are randomly selected in the plant for growing up to seedling as experiment pair
As weighing respectively, taking the average value of 10 plants of seedling qualities as the seedling average quality before being dried, be dried
Afterwards, it still weighs respectively, takes the average value of 10 plants of seedling qualities as the seedling average quality after being dried；
S5 calculates Drought tolerance index according to following formula:
F=(Y1/Y2+ (WB1-WB2)/(WA1-WA2)+M1/M2) × 10；
If F >=25, which is that height is drought-enduring；If 15≤F < 25, the kind is generally drought-enduring, should if F < 15
Kind is not drought-enduring.
No. 2 radish varieties experimental results of Fuling red heart: Y1 63%, Y2 99%, WA1 7.0g, WA2 2.2g, WB1
For 7.3g, WB2 3.1g, M1 99%, M2 100%.
F=(Y1/Y2+ (WB1-WB2)/(WA1-WA2)+M1/M2) × 10=(63%/99%+ (7.3-3.1)/(7.0-
2.2)+82%/100%) × 10=23.3.
It is identified through this method, red heart No. 2 radish in Fuling are general drought-enduring variety, consistent with result generally acknowledged at present.
Embodiment 2
A kind of method of radish drought tolerance Rapid identification, radish germ plasm resource are autumn white No. 65 white radishes (not drought-enduring radish
Kind, Lanzhou local varieties), comprising the following steps:
Radish seed is divided into A group and B group by S1, and the quantity of A group and B group radish is 400, wherein for the plantation of A group in
In arid soil, B group is planted in normal moisture content soil；The water content of arid soil is 10~15% (mass fractions), just
The water content of normal water content soil is 60~70% (mass fractions)；It is 50 that arid soil and normal moisture content soil, which are separated by spacing,
Rice maintains the water content of normal soil and arid soil by way of manual priming；Radish kind by the way of digging cave dibbling
It plants, one, every cave seed, spacing in the rows 10cm, line-spacing 20cm, which is also convenient for whole seedlings of choosing convenient for record statistics.
S2 measures the germination percentage Y2 of germination percentage Y1 and the B group of A group respectively, and the criterion of germination is: 2 cotyledons are grown,
Cotyledon is emerged 5~10mm of soil；
The measuring method of germination percentage is as follows: using 200 seeds as calculating basis C1, germinable seed amount note after plantation
For C2, germination percentage=C2/C1；
S3, field management, when radish growth to 7 true leaf, that is, fleshy roots breaks tripe, count the seedling percent M1 of A group with
Seedling percent M2, the A group and B component of B group do not extract a part of whole seedlings, spare；It is long in seedling percent=germination strain
At strain number total amount/germination strain number total amount of seedling；
S4 measures A group seedling average quality WA1, measures B group seedling average quality WB1, then by A group seedling, B group children
Seedling is placed under 40 DEG C of ventilated environments and is dried 4h, measures A group seedling average quality WA2 again, measures B group seedling and is averaged matter
Measure WB2；
The calculation method of seedling average quality is as follows: 5 plants randomly selected in the plant for growing up to seedling is used as experimental subjects,
It weighs respectively, takes the average value of 5 plants of seedling qualities as the seedling average quality before being dried, after being dried, still
It so weighs respectively, takes the average value of 5 plants of seedling qualities as the seedling average quality after being dried；
S5 calculates Drought tolerance index according to following formula:
F=(Y1/Y2+ (WB1-WB2)/(WA1-WA2)+M1/M2) × 10；
If F >=25, which is that height is drought-enduring；If 15≤F < 25, the kind is generally drought-enduring, should if F < 15
Kind is not drought-enduring.
Autumn white No. 65 white radishes kind experimental result: Y1 39%, Y2 100%, WA1 9.5g, WA2 4.0g, WB1
For 9.3g, WB2 5.9g, M1 36%, M2 99%.
F=(Y1/Y2+ (WB1-WB2)/(WA1-WA2)+M1/M2) × 10=(39%/100%+ (9.3-5.9)/(9.5-
4.0)+36%/99%) × 10=13.7.
It is identified through this method, No. 65 white radishes of Qiu Bai are not drought-enduring variety, consistent with result generally acknowledged at present.
Embodiment 3
A kind of method of radish drought tolerance Rapid identification, radish germ plasm resource are No. 1 radish (general drought tolerance of Fuling red heart
Radish varieties), comprising the following steps:
Radish seed is divided into A group and B group by S1, and the quantity of A group and B group radish is 200, wherein for the plantation of A group in
In arid soil, B group is planted in normal moisture content soil；The water content of arid soil is 15~20% (mass fractions), just
The water content of normal water content soil is 30~40% (mass fractions)；It is 40 that arid soil and normal moisture content soil, which are separated by spacing,
Rice maintains the water content of normal soil and arid soil by way of manual priming；
S2 measures the germination percentage Y2 of germination percentage Y1 and the B group of A group respectively, and the criterion of germination is: 2 cotyledons are grown,
Cotyledon is emerged 5~10mm of soil；
The measuring method of germination percentage is as follows: using 150 seeds as calculating basis C1, germinable seed amount note after plantation
For C2, germination percentage=C2/C1；
S3, field management, when grow to 8 true leaves i.e. fleshy root break tripe when, count A group seedling percent M1 and B group
Seedling percent M2, A group and B component do not extract a part of whole seedlings, it is spare；Seedling percent=germination strain number is total
Grow up to the strain number total amount of seedling in amount/germination strain；
S4 measures A group seedling average quality WA1, measures B group seedling average quality WB1, then by A group seedling, B group children
Seedling is placed under 45 DEG C of ventilated environments and is dried 4h, measures A group seedling average quality WA2 again, measures B group seedling and is averaged matter
Measure WB2；
The calculation method of seedling average quality is as follows: 10 plants are randomly selected in the plant for growing up to seedling as experiment pair
As weighing respectively, taking the average value of 10 plants of seedling qualities as the seedling average quality before being dried, be dried
Afterwards, it still weighs respectively, takes the average value of 10 plants of seedling qualities as the seedling average quality after being dried；
S5 calculates Drought tolerance index according to following formula:
F=(Y1/Y2+ (WB1-WB2)/(WA1-WA2)+M1/M2) × 10；
If F >=25, which is that height is drought-enduring；If 15≤F < 25, the kind is generally drought-enduring, should if F < 15
Kind is not drought-enduring.
No. 1 radish varieties experimental result of Fuling red heart: Y1 60.7%, Y2 98.5%, WA1 9.9g, WA2 are
4.6g, WB1 10.1g, WB2 5.4g, M1 90%, M2 99%.
F=(Y1/Y2+ (WB1-WB2)/(WA1-WA2)+M1/M2) × 10=(60.7%/98.5%+ (10.1-5.4)/
(9.9-4.6)+95.1%/99%) × 10=24.6.
It is identified through this method, red heart No. 1 radish in Fuling is general drought-enduring variety, consistent with result generally acknowledged at present.
Embodiment 4
A kind of method of radish drought tolerance Rapid identification, radish germ plasm resource are autumn white No. 65 white radishes (not drought-enduring radish
Kind, Lanzhou local varieties), No. 65 white radishes of Qiu Bai are first impregnated 8 hours with clear water, spare again after draining away the water including following
Step:
Radish seed is divided into A group and B group by S1, and the quantity of A group and B group radish is 400, wherein for the plantation of A group in
In arid soil, B group is planted in normal moisture content soil；The water content of arid soil is 10~15% (mass fractions), just
The water content of normal water content soil is 60~70% (mass fractions)；It is 50 that arid soil and normal moisture content soil, which are separated by spacing,
Rice maintains the water content of normal soil and arid soil by way of manual priming；Radish kind by the way of digging cave dibbling
It plants, one, every cave seed, spacing in the rows 10cm, line-spacing 20cm, which is also convenient for whole seedlings of choosing convenient for record statistics.
S2 measures the germination percentage Y2 of germination percentage Y1 and the B group of A group respectively, and the criterion of germination is: 2 cotyledons are grown,
Cotyledon is emerged 5~10mm of soil；
The measuring method of germination percentage is as follows: using 200 seeds as calculating basis C1, germinable seed amount note after plantation
For C2, germination percentage=C2/C1；
S3, field management, when grow to 7 true leaves i.e. fleshy root break tripe when, count A group seedling percent M1 and B group
Seedling percent M2, A group and B component do not extract a part of whole seedlings, it is spare；Grow up to children in seedling percent=germination strain
Strain number total amount/germination strain number total amount of seedling；
S4 measures A group seedling average quality WA1, measures B group seedling average quality WB1, then by A group seedling, B group children
Seedling is placed under 40 DEG C of ventilated environments and is dried 4h, measures A group seedling average quality WA2 again, measures B group seedling and is averaged matter
Measure WB2；
The calculation method of seedling average quality is as follows: 5 plants randomly selected in the plant for growing up to seedling is used as experimental subjects,
It weighs respectively, takes the average value of 5 plants of seedling qualities as the seedling average quality before being dried, after being dried, still
It so weighs respectively, takes the average value of 5 plants of seedling qualities as the seedling average quality after being dried；
S5 calculates Drought tolerance index according to following formula:
F=(Y1/Y2+ (WB1-WB2)/(WA1-WA2)+M1/M2) × 10；
If F >=25, which is that height is drought-enduring；If 15≤F < 25, the kind is generally drought-enduring, should if F < 15
Kind is not drought-enduring.
Autumn white No. 65 white radishes kind experimental result: Y1 55%, Y2 100%, WA1 9.0g, WA2 4.1g, WB1
For 8.9g, WB2 5.7g, M1 29.3%, M2 100%.
F=(Y1/Y2+ (WB1-WB2)/(WA1-WA2)+M1/M2) × 10=(55%/100%+ (8.9-5.7)/(9.0-
4.1)+29.3%/100%) × 10=14.1.
It is identified through this method, No. 65 white radishes of Qiu Bai are not drought-enduring variety, consistent with result generally acknowledged at present.
It should be noted that described in the embodiment of the present invention 1~4 and " field management " mode it is as follows: take and normally apply
Fertilizer, weeding, the operation such as plough, suitably pour water when only pouring water according to soil water content demand.
It should be noted that when the present invention provides numberical range, it should be appreciated that except non-present invention is otherwise noted, every number
Being worth any one numerical value between two endpoints and two endpoints of range can be selected.Unless otherwise defined, make in the present invention
All technical and scientific terms are identical as the normally understood meaning of those skilled in the art of the present technique.
Although preferred embodiments of the present invention have been described, it is created once a person skilled in the art knows basic
Property concept, then additional changes and modifications may be made to these embodiments.So it includes excellent that the following claims are intended to be interpreted as
It selects embodiment and falls into all change and modification of the scope of the invention.
Obviously, various changes and modifications can be made to the invention without departing from essence of the invention by those skilled in the art
Mind and range.In this way, if these modifications and changes of the present invention belongs to the range of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to include these modifications and variations.
Claims (8)
1. a kind of method of radish drought tolerance Rapid identification, which comprises the following steps:
Radish seed is divided into A group and B group by S1, wherein being the plantation of A group in arid soil, the plantation of B group is in normal moisture content soil
In earth；
S2 measures the germination percentage Y2 of germination percentage Y1 and the B group of A group respectively, and the criterion of germination is: growing 2 cotyledons, and son
Leaf is emerged 5~10mm of soil；
S3, field management count the seedling percent M1 and B of A group when radish growth breaks tripe to 5~8 true leaf, that is, fleshy roots
Seedling percent M2, the A group and B component of group do not extract a part of whole seedlings, spare；
S4 measures A group seedling average quality WA1, measures B group seedling average quality WB1, then that A group seedling, B group seedling is equal
It is placed under 30~45 DEG C of ventilated environments and is dried 4h, measure A group seedling average quality WA2 again, measure B group seedling and be averaged matter
Measure WB2；
S5 calculates Drought tolerance index according to following formula:
F=(Y1/Y2+ (WB1-WB2)/(WA1-WA2)+M1/M2) × 10；
If A group germination percentage is 0, the value of (WA1-WA2)/(WB1-WB2) is denoted as 0, no longer needs to the step of carrying out S4；
If F >=25, which is that height is drought-enduring；If 18≤F < 25, the kind is generally drought-enduring, if F < 18, the kind
It is not drought-enduring.
2. the method for radish drought tolerance Rapid identification according to claim 1, which is characterized in that the water content of arid soil
It is 10~20%, the water content of normal moisture content soil is 30~70%.
3. the method for radish drought tolerance Rapid identification according to claim 1, which is characterized in that the number of A group and B group radish
Amount is 200~300.
4. the method for radish drought tolerance Rapid identification according to claim 1, which is characterized in that the measuring method of germination percentage
As follows: using 100 seeds as calculating basis C1, germinable seed amount is denoted as C2, germination percentage=C2/C1 after plantation.
5. the method for radish drought tolerance Rapid identification according to claim 1, which is characterized in that the calculating of seedling percent
Method is as follows: growing up to strain number total amount/germination strain number total amount of seedling in seedling percent=germination strain.
6. the method for radish drought tolerance Rapid identification according to claim 1, which is characterized in that the meter of seedling average quality
Calculation method is as follows:
10 plants are randomly selected in the plant for growing up to seedling as experimental subjects, are weighed respectively, are taken being averaged for 10 plants of seedling qualities
It is worth as the seedling average quality before being dried；It after being dried, still weighs respectively, takes the flat of 10 plants of seedling qualities
Mean value is as the seedling average quality after being dried.
7. the method for radish drought tolerance Rapid identification according to claim 1, which is characterized in that arid soil and normally contain
It is 30~50 meters that water soil, which is separated by spacing, and the water content of soil is maintained by way of manual priming.
8. the method for radish drought tolerance Rapid identification according to claim 1, which is characterized in that radish is using digging cave dibbling
Mode plant, one, every cave seed, 5~15cm of spacing in the rows, 10~20cm of line-spacing.
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| CN111257509A (en) * | 2020-01-18 | 2020-06-09 | 吉林省农业科学院 | Method for drought tolerance identification of corn in germination period |
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| CN106818101A (en) * | 2016-07-29 | 2017-06-13 | 新疆农业科学院粮食作物研究所 | A kind of method for identifying drought resistance of maize |
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| CN111257509A (en) * | 2020-01-18 | 2020-06-09 | 吉林省农业科学院 | Method for drought tolerance identification of corn in germination period |
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