CN109392350A - A method of improving corn seed germination gesture and vitality index - Google Patents
A method of improving corn seed germination gesture and vitality index Download PDFInfo
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- CN109392350A CN109392350A CN201811185144.3A CN201811185144A CN109392350A CN 109392350 A CN109392350 A CN 109392350A CN 201811185144 A CN201811185144 A CN 201811185144A CN 109392350 A CN109392350 A CN 109392350A
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- corn seed
- filter paper
- seed
- vitality index
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
- A01C1/025—Testing seeds for determining their viability or germination capacity
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- Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a kind of methods for improving corn seed germination gesture and vitality index, include the following steps: that (1) sterilizes corn seed, with sterile water wash, are soaked in 22-26h in water；By in two layers of filter paper paving container after sterilization, with concentration be 15-25mmol/L NaCl solution immersion described in filter paper；(3) corn seed that step (1) obtains is placed on the filter paper that step (2) obtains in the upward mode of seed embryo, covers one layer of filter paper, cultivated, obtain germinating energy and seedling that vitality index all improves.Method of the invention is simple and easy, and the synthesis sprouting state of corn seed can be improved.
Description
Technical field
The present invention relates to plant seed germination technical fields, and in particular on the basis of guaranteeing corn seed germination rate,
A method of improving corn seed germination gesture and vitality index.
Background technique
Corn is one of most important cereal crops of the mankind.In world cereal crops, corn total output first, volume of trade
Second.The sprouting of corn seed is the important turning point of its growth cycle and the critical stage of seedling establishment.Seed is sprouted
Seed can be not only saved in order, reduces input cost, and also ensure planting density, determine the more of strain yield
It is few.Meanwhile seed sprouting state also be used to measure the important indicator of kind superiority and inferiority.But at present due to corn seed preserving type
The drawbacks of, so that seed is chronically at the state of dry suspend mode, even if seed by immersion in advance being difficult that seed is made to reach preferable
Sprouting state, therefore improve seed sprouting state such as germinating energy and vitality index be a very eager problem.
Summary of the invention
The purpose of the present invention is overcoming the deficiencies of the prior art and provide on the basis of guaranteeing corn seed germination rate, one
The method that kind improves corn seed germination gesture and vitality index.
Technical solution of the present invention is summarized as follows:
A method of corn seed germination gesture and vitality index are improved, is included the following steps:
(1) corn seed is sterilized, with sterile water wash, is soaked in 22-26h in water；
(2) two layers of filter paper is spread in container after sterilization, the NaCl solution immersion institute for being 15-25mmol/L with concentration
State filter paper；
(3) corn seed that step (1) obtains is placed in the filter paper that step (2) obtains in the upward mode of seed embryo
On, one layer of filter paper is covered, is cultivated, obtains germinating energy and seedling that vitality index all improves.
The preferred 20mmol/L of the concentration of NaCl aqueous solution.
The condition of culture are as follows: at 22-28 DEG C, daily lighting delay number is 12h, and number is 12h when dark, and relative humidity is
60%, intensity of illumination 4000lux.
Beneficial effects of the present invention: when NaCl concentration is 15-25mmo/L, there is no by shadow for corn seed germination rate
It rings, and the germinating energy of corn seed and vitality index are significantly higher than germinating energy and vitality index when NaCl concentration is 0mmol/L.
The higher uniformity for indicating that seed is sprouted of germinating energy is higher and germination period is shorter, is conducive to the growth of seedling；Seed
Vitality index is the potentiality of germination and emergence rate, growth of seedling, is the important indicator of seed quality.Method letter of the invention
It is single easy, the synthesis sprouting state of corn seed can be improved.
Detailed description of the invention
Fig. 1 is influence of the difference NaCl concentration of aqueous solution to corn seed germination rate in embodiment 1；
Fig. 2 is influence of the difference NaCl concentration of aqueous solution to corn seed germination gesture in embodiment 1；
Fig. 3 is influence of the difference NaCl concentration of aqueous solution to Vigor of Zea mays L. Seeds index in embodiment 1；
Fig. 4 is influence of the difference NaCl concentration of aqueous solution to corn seed germination rate in embodiment 2；
Fig. 5 is influence of the difference NaCl concentration of aqueous solution to corn seed germination gesture in embodiment 2；
Fig. 6 is influence of the difference NaCl concentration of aqueous solution to Vigor of Zea mays L. Seeds index in embodiment 2.
Specific embodiment
Corn seed: the day tower 619 bought from Tianjin ZTDD-Geneseed Technology Co., Ltd..
Above-mentioned raw materials source is in order to enable those skilled in the art to more fully understand the present invention, but not to this hair
It is bright to limit.
Embodiment 1
A method of corn seed germination gesture and vitality index are improved, is included the following steps:
(1) in the same size, liquor natrii hypochloritis's disinfection of 619 corn seed of the day tower 10g/L of full seed is chosen
15min is cleaned repeatedly with sterile water, is soaked in water for 24 hours；(it is demonstrated experimentally that any number being soaked in water between 22-26h is all
Can be with) (2) spread two layers of filter paper in container after sterilization, with concentration be respectively 0mmol/L, 15mmol/L, 30mmol/L,
Filter paper described in the NaCl solution immersion of 45mmol/L, 60mmol/L；
(3) corn seed that step (1) obtains is placed in the filter paper that step (2) obtains in the upward mode of seed embryo
On, each container puts 55, covers one layer of filter paper, be retained moisturizing, every kind of concentration in triplicate, at 22-28 DEG C, daily illumination
Number is 12h, and number is 12h when dark, and relative humidity 60%, intensity of illumination is that 4000lux is cultivated 7 days, obtains germinating energy and work
The seedling that power index all improves.
Main root length is germination standard when reaching seed length half, counts germinative number daily and simultaneously records.It is surveyed after seven days
It measures single seeded dry weight and calculates every germination index of seed.
The measurement for index of germinateing:
(1) germination percentage=N/55 × 100%；N indicates the 7th day germinative number.
(2) germinating energy=M/55 × 100%；M indicates the 4th day germinative number.
(3) vitality index=germinating energy × simple grain chitting piece dry weight
The weighing of dry weight: when germination test carries out the 8th day, randomly selecting the seed of 10 grain germinations, 105 DEG C of water-removing 30min,
It dries to constant weight for 80 DEG C, weighs later.
See Fig. 1,2,3.
Interpretation of result:
For corn seed when NaCl concentration of aqueous solution is 0-30mmol/L, there is no significant for germination percentage as can be seen from Figure 1
Variation, is all 90% or so, when NaCl concentration of aqueous solution is in 45-60mmol/L, germination percentage is substantially less than 90%.
From Fig. 2,3 as can be seen that the germinating energy and vitality index of corn seed in NaCl concentration of aqueous solution are 15mmol/L
When occur a peak value, substantially it is expected that NaCl concentration of aqueous solution corn seed within the scope of 0-30mmol/L germination
Gesture and vitality index first increase to be declined afterwards, but which point specifically reaches peak at can not determine.
In conclusion the germination percentage of corn seed is not by significant when NaCl concentration of aqueous solution is 0-30mmol/L
Influence, but the germinating energy of corn seed and vitality index have it is dramatically different.It was determined that being in NaCl concentration of aqueous solution
When 15mmol/L or so, the germinating energy and vitality index of corn seed are significantly improved relative to other concentration for the treatment of.
Embodiment 2
A method of corn seed germination gesture and vitality index are improved, is included the following steps:
(1) in the same size, liquor natrii hypochloritis's disinfection of 619 corn seed of the day tower 10g/L of full seed is chosen
15min is cleaned repeatedly with sterile water, is soaked in water for 24 hours (it is demonstrated experimentally that any number being soaked in water between 22-26h is all
It can be with)；
(2) by two layers of filter paper paving container after sterilization, with concentration be respectively 0mmol/L, 5mmol/L, 10mmol/L,
Filter paper described in the NaCl solution immersion of 15mmol/L, 20mmol/L, 25mmol/L, 30mmol/L；
(3) corn seed that step (1) obtains is placed in the filter paper that step (2) obtains in the upward mode of seed embryo
On, each container puts 55, covers one layer of filter paper, be retained moisturizing, every kind of concentration in triplicate, at 22-28 DEG C, daily illumination
Number is 12h, and number is 12h when dark, and relative humidity 60%, intensity of illumination is that 4000lux is cultivated 7 days, obtains germinating energy and work
The seedling that power index all improves.
Main root length is germination standard when reaching seed length half, counts germinative number daily and simultaneously records.It is surveyed after seven days
It measures single seeded dry weight and calculates every germination index of seed.
The measurement for index of germinateing:
(1) germination percentage=N/55 × 100%；N indicates the 7th day germinative number.
(2) germinating energy=M/55 × 100%；M indicates the 4th day germinative number.
(3) vitality index=germinating energy × simple grain chitting piece dry weight
The weighing of dry weight: when germination test carries out the 8th day, randomly selecting the seed of 10 grain germinations, 105 DEG C of water-removing 30min,
It dries to constant weight for 80 DEG C, weighs later.See Fig. 4,5,6.
Interpretation of result:
From Fig. 4 can, after 0-30mmol/L is specifically divided after various concentration gradient, germination percentage also becomes there is no significant
Change, is all 90% or so；This shows not influence corn seed germination rate in low NaCl concentration of aqueous solution.
From fig. 5, it can be seen that the germinating energy of corn seed reaches highest when NaCl concentration of aqueous solution is in 20mmol/L
85.45% or so, be significantly higher than concentration be 15mmol/L (78.18%) and 25mmol/L (74.55%) germinating energy.From figure
Above it is also seen that the germinating energy of corn seed is also at optimum range when NaCl concentration of aqueous solution is in 15-25mmol/L.
The conclusion similar with germinating energy as can be drawn from Figure 6, when NaCl concentration of aqueous solution is in 15-25mmol/L, corn
The vitality index of seed is also at optimum range, while when concentration is 20mmol/L, the vitality index of corn seed reaches most
It is high.In conclusion under low NaCl concentration of aqueous solution, corn seed germination rate does not have significantly affected, but corn seed
Germinating energy and vitality index all present dramatically different；When NaCl solution concentration is in 20mmol/L, corn seed germination gesture and
Vitality index reaches highest；When NaCl solution concentration is in 15-25mmol/L, the germinating energy of corn seed is also at best model
It encloses.
Claims (3)
1. a kind of method for improving corn seed germination gesture and vitality index, it is characterized in that including the following steps:
(1) corn seed is sterilized, with sterile water wash, is soaked in 22-26h in water；
(2) by two layers of filter paper paving container after sterilization, with concentration be 15-25mmol/L NaCl solution immersion described in filter
Paper；
(3) corn seed that step (1) obtains is placed on the filter paper that step (2) obtains in the upward mode of seed embryo, is covered
One layer of filter paper of lid, culture obtain germinating energy and seedling that vitality index all improves.
2. according to the method described in claim 1, it is characterized in that the concentration of the NaCl aqueous solution is 20mmol/L.
3. according to the method described in claim 1, it is characterized in that the condition of the culture are as follows: at 22-28 DEG C, daily lighting delay number
For 12h, number is 12h, relative humidity 60%, intensity of illumination 4000lux when dark.
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| CN201811185144.3A CN109392350A (en) | 2018-10-11 | 2018-10-11 | A method of improving corn seed germination gesture and vitality index |
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| CN201811185144.3A CN109392350A (en) | 2018-10-11 | 2018-10-11 | A method of improving corn seed germination gesture and vitality index |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112655308A (en) * | 2019-10-16 | 2021-04-16 | 东北农业大学 | Method for stabilizing germination potential of corn seeds |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2565260C1 (en) * | 2014-05-29 | 2015-10-20 | Государственное научное учреждение Поволжский научно-исследовательский институт производства и переработки мясомолочной продукции Российской академии сельскохозяйственных наук | Method of regulation of plant growth |
-
2018
- 2018-10-11 CN CN201811185144.3A patent/CN109392350A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2565260C1 (en) * | 2014-05-29 | 2015-10-20 | Государственное научное учреждение Поволжский научно-исследовательский институт производства и переработки мясомолочной продукции Российской академии сельскохозяйственных наук | Method of regulation of plant growth |
Non-Patent Citations (4)
| Title |
|---|
| 卢海强等: "琼脂固定法盐胁迫对玉米种子萌发和幼苗生长的影响 ", 《广东农业科学》 * |
| 陈丽珍等: "NaCl胁迫对不同玉米品种种子萌发特性的影响", 《安徽农业科学》 * |
| 陈小文等: "NaCl浸种对转Bt基因玉米与受体品种萌发及幼苗生长形态的影响", 《华北农学报》 * |
| 高英等: "盐胁迫对玉米发芽和苗期生长的影响", 《中国土壤与肥料》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112655308A (en) * | 2019-10-16 | 2021-04-16 | 东北农业大学 | Method for stabilizing germination potential of corn seeds |
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