CN109496685A - A kind of cultural method of umbellate pore furgus - Google Patents
A kind of cultural method of umbellate pore furgus Download PDFInfo
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- CN109496685A CN109496685A CN201811630065.9A CN201811630065A CN109496685A CN 109496685 A CN109496685 A CN 109496685A CN 201811630065 A CN201811630065 A CN 201811630065A CN 109496685 A CN109496685 A CN 109496685A
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- 239000011148 porous material Substances 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 25
- 239000002689 soil Substances 0.000 claims abstract description 71
- 238000012545 processing Methods 0.000 claims abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 238000005516 engineering process Methods 0.000 claims abstract description 10
- 238000012360 testing method Methods 0.000 claims description 49
- 239000001963 growth medium Substances 0.000 claims description 35
- 239000007788 liquid Substances 0.000 claims description 35
- 239000002023 wood Substances 0.000 claims description 20
- 241000196324 Embryophyta Species 0.000 claims description 15
- 244000061456 Solanum tuberosum Species 0.000 claims description 15
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 15
- 239000002699 waste material Substances 0.000 claims description 15
- 241000576755 Sclerotia Species 0.000 claims description 10
- 238000011081 inoculation Methods 0.000 claims description 10
- 235000007164 Oryza sativa Nutrition 0.000 claims description 7
- 235000009566 rice Nutrition 0.000 claims description 7
- 238000001914 filtration Methods 0.000 claims description 6
- 229920001817 Agar Polymers 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 239000008272 agar Substances 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 5
- 238000004090 dissolution Methods 0.000 claims description 5
- 239000012153 distilled water Substances 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 238000005259 measurement Methods 0.000 claims description 5
- 238000011177 media preparation Methods 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 5
- 230000011218 segmentation Effects 0.000 claims description 5
- 239000002904 solvent Substances 0.000 claims description 5
- 241000894007 species Species 0.000 claims description 5
- 238000012549 training Methods 0.000 claims description 5
- 241001558929 Sclerotium <basidiomycota> Species 0.000 claims description 4
- 241001251949 Xanthium sibiricum Species 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims 1
- 239000005416 organic matter Substances 0.000 claims 1
- 238000009395 breeding Methods 0.000 abstract description 2
- 230000001488 breeding effect Effects 0.000 abstract description 2
- 239000003814 drug Substances 0.000 abstract description 2
- 238000007654 immersion Methods 0.000 abstract 1
- 241000209094 Oryza Species 0.000 description 6
- 241000282898 Sus scrofa Species 0.000 description 3
- 235000019994 cava Nutrition 0.000 description 3
- 238000009423 ventilation Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 241000222382 Agaricomycotina Species 0.000 description 1
- 244000144730 Amygdalus persica Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000222341 Polyporaceae Species 0.000 description 1
- 241000222640 Polyporus Species 0.000 description 1
- 244000171085 Polyporus umbellatus Species 0.000 description 1
- 235000004837 Polyporus umbellatus Nutrition 0.000 description 1
- 244000197580 Poria cocos Species 0.000 description 1
- 235000008599 Poria cocos Nutrition 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- 230000007969 cellular immunity Effects 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 239000003501 hydroponics Substances 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 230000007721 medicinal effect Effects 0.000 description 1
- 230000000242 pagocytic effect Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000003307 reticuloendothelial effect Effects 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 230000004614 tumor growth Effects 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
A kind of cultural method of umbellate pore furgus, is related to Chinese medicine artificial breeding technique field, and this method includes preparatory technology step, planting operations processing step before planting.Cultivation operation: about 40 centimetres of diameter or more cylindric soil pits are dug in soil, soil pit depth is longer than 5-8 centimetres of batten, one layer of broad-leaved impregnated in water more than 3 hours is placed under cave, broad-leaved is chosen in plantation area, 4-6 root batten is stood on into jam-packed in soil pit, form a timber cylindrical body, periphery soil landfill；After 500 grams, the 500 grams halimasch of broad-leaved of immersion more than 3 hours, 500 grams of domestications grams of umbellate pore furgus cultivar is suitably mixed thoroughly, is squeezed into the gap for erectting the centre of timber；With soil covering on cave, the little Tu Bao that a height is no more than 10 centimetres is formed；The distance between cave and cave are at 10 centimetres or more when plantation；It is every square metre general, the cave 3-4 can be planted.
Description
Technical field
The present invention relates to Chinese medicine artificial breeding technique field, the cultural methods of specifically a kind of umbellate pore furgus.
Background technique
Umbellate pore furgus (Polyporus umbellatus), alias pig dung bacterium, wild boar Siberian cocklebur, pig Poria cocos, dendropolyporus umbellatus, black peach etc.,
Belong to Basidiomycotina, Hymenomycetes, Polyporaceae, Polyporus.Have the medicinal history of two thousand years in China, is that tradition is medicinal
One of fungi.The medicinal effects of umbellate pore furgus are the sclerotium of umbellate pore furgus.Entire sclerotium is interwoven by a large amount of white hyphas, in mycelia
Sky, it is superfine and short.Sclerotium is brownish black or dark brown in irregular bulk, surface, there is scraggly strumae and wrinkle.
Near-white or faint yellow in core is hardened after dry.Recent study proves that contained polysaccharide in sclerotia is a kind of non-specific
The cellular immunity stimulant of property, can significantly reinforce reticuloendothelial cell phagocytic function, have inhibiting effect to tumour growth, by
Domestic and international most attention, so the scale of artificial growth umbellate pore furgus is also increasing, but the existing implantation methods maturity period is long, produces
It measures low, is not able to satisfy growing consumption demand.
Summary of the invention
Technical problem to be solved by the invention is to provide a kind of cultural methods of umbellate pore furgus, have solved the prior art maturity period
The problem of length, low output.
The technical solution of present invention solution technical problem are as follows: a kind of cultural method of umbellate pore furgus, this method include standard before plantation
Standby processing step, planting operations processing step.
Preparatory technology step includes before the plantation: umbellate pore furgus strain domestication, waste wood processing are planted in vertical stake.
The stake plants the concrete operations of umbellate pore furgus strain domestication and includes: PDA culture medium preparation, the inoculation of nucleome tissue and cultivate.
Prepared by PDA culture medium, for a PDA culture medium.
Weigh 50-70 grams of sawdust, 150-200 g potato；Potato is switched to thickness less than 5 millimeters, lateral longitudinal length
It rains 5 centimetres；Sawdust, the potato block cut are added 1000 milliliters of water, 100 degrees Celsius of sealings are boiled 10-15 minutes, and gauze is used
Filtering, obtains level-one auxiliary liquid, the level-one auxiliary liquid of filtering is no less than 1000 milliliters.
10-20 grams of glucose, 17-20 grams of agar are added into level-one auxiliary liquid, second level auxiliary liquid is obtained after completely dissolution, by two
Grade auxiliary liquid is distributed into test tube, and about 5-10 milliliters of every test tube, for test tube opening diameter less than 4 centimetres, second level auxiliary liquid accounts for test tube solvent not
20 the percent of test tube total measurement (volume) can be less than.
It will be put into equipped with second level auxiliary liquid test tube, sterilize 20-30 minutes in 121 degrees Celsius of environment, and take out test tube, test tube inclination
30-40 degree is tiltedly put, and spare, as a PDA culture medium in each test tube after cooling is stored.
The inoculation of nucleome tissue and cultivation.
A PDA culture medium is taken, is placed in the empty culture dish of 10 centimetres of diameter, it aseptically will be fresh
Sclerotia nucleome tissue segmentation at 5 millimeters of square fritters below, every part of PDA culture medium places 3 pieces of sclerotias
Nucleome tissue is set and is cultivated under room temperature 23-26 degrees celsius at triangle.
After 28-33 days, the strain of picking stalwartness mycelia is transferred in the test tube equipped with 1 part of PDA culture medium, sets room temperature 23-
Training is formed until umbellate pore furgus parent species under 26 degrees celsius, that is, the umbellate pore furgus cultivar after taming.
Waste wood processing.
10-20 centimetres of diameter of waste wood is sawn into long 55-65 centimetres of batten, is impregnated 2-3 days in distilled water, after pulling out
It is placed 8-12 hours in aeration-drying sublimity.
The planting operations technique includes as follows.
Plant the time to choose: in annual 3-5 month, soil temperature can choose soil at 5-15 degrees Celsius and start to cultivate.
Choose in plantation area: cultivation should be selected in height above sea level at 1000-1600 meters, and on 0.5 meter, soil has soil thickness
Machine matter content is between 8% or more, soil pH value 5.5-7.2, and soil year-round average temperature is in 7-15 degrees Celsius of leaf wood woods or woods
On area, soil, periphery.
Cultivation operation: digging about 40 centimetres of diameter or more cylindric soil pits, soil pit depth in soil and be longer than 5-8 centimetres of batten,
One layer of broad-leaved impregnated in water more than 3 hours is placed under cave, broad-leaved is chosen in plantation area, and 4-6 root batten is stood on soil
Jam-packed in cave forms a timber cylindrical body, periphery soil landfill；500 grams of broad-leaved, 500 grams of sweet rings more than 3 hours will be impregnated
Bacterium, 500 grams domestication after gram umbellate pore furgus cultivar suitably mix thoroughly, be squeezed into erect timber centre gap in；It is covered on cave with soil
Lid forms the little Tu Bao that a height is no more than 10 centimetres；The distance between cave and cave are at 10 centimetres or more when plantation；It is general every
Square metre, the cave 3-4 can be planted.
Benefit effect of the invention is: vertical stake hydroponics is within umbellate pore furgus growth period, without watering and fertilising.Only often go
Observation, takes out weeds on cave.Winter can cover one layer of leaf of 10cm or so on cave.General 3 years cultivation periods.Three
It can just be received after year, and new one batch of sowing again.Peasant can arrange in labour gap, harvest after mature, be not necessarily to strong labour power,
The effect got rich and increased income can be played.
Specific embodiment
The present invention will be further described with reference to embodiments.
Embodiment 1.
A kind of cultural method of umbellate pore furgus, this method include preparatory technology step, planting operations processing step before planting.
Preparatory technology step includes before the plantation: umbellate pore furgus strain domestication, waste wood processing are planted in vertical stake.
The stake plants the concrete operations of umbellate pore furgus strain domestication and includes: PDA culture medium preparation, the inoculation of nucleome tissue and cultivate.
Prepared by PDA culture medium, for a PDA culture medium.
Weigh 70 grams of sawdusts, 200 g potatos；Potato is switched to thickness less than 5 millimeters, and lateral longitudinal length rains 5 lis
Rice；Sawdust, the potato block cut are added 1000 milliliters of water, 100 degrees Celsius of sealings are boiled 15 minutes, with filtered through gauze, are obtained
The level-one auxiliary liquid of level-one auxiliary liquid, filtering is no less than 1000 milliliters.
20 grams of glucose, 20 grams of agar are added into level-one auxiliary liquid, second level auxiliary liquid are obtained after completely dissolution, by second level auxiliary liquid
It is distributed into test tube, about 10 milliliters of every test tube, for test tube opening diameter less than 4 centimetres, second level auxiliary liquid, which accounts for test tube solvent, cannot be less than examination
20 the percent of pipe total measurement (volume).
It will be put into equipped with second level auxiliary liquid test tube, sterilize 30 minutes in 121 degrees Celsius of environment, and take out test tube, test tube inclination 40
Degree is tiltedly put, and spare, as a PDA culture medium in each test tube after cooling is stored.
The inoculation of nucleome tissue and cultivation.
A PDA culture medium is taken, is placed in the empty culture dish of 10 centimetres of diameter, it aseptically will be fresh
Sclerotia nucleome tissue segmentation at 5 millimeters of square fritters below, every part of PDA culture medium places 3 pieces of sclerotias
Nucleome tissue is set and is cultivated under 26 degrees celsius of room temperature at triangle.
After 33 days, the strain of picking stalwartness mycelia is transferred in the test tube equipped with 1 part of PDA culture medium, and it is Celsius to set room temperature 26
Training is formed until umbellate pore furgus parent species under the conditions of degree, that is, the umbellate pore furgus cultivar after taming.
Waste wood processing.
20 centimetres of waste wood of diameter is sawn into long 65 centimetres of battens, is impregnated 3 days in distilled water, it is dry in ventilation after pulling out
It is placed 12 hours in dry sublimity.
The planting operations technique includes as follows.
Plant the time to choose: in annual 3-5 month, soil temperature can choose soil at 5-15 degrees Celsius and start to cultivate.
Choose in plantation area: cultivation should be selected in height above sea level at 1000-1600 meters, and on 0.5 meter, soil has soil thickness
Machine matter content is between 8% or more, soil pH value 5.5-7.2, and soil year-round average temperature is in 7-15 degrees Celsius of leaf wood woods or woods
On area, soil, periphery.
Cultivation operation: digging about 40 centimetres of diameter or more cylindric soil pits, soil pit depth in soil and be longer than 8 centimetres of batten,
One layer of broad-leaved impregnated in water more than 3 hours is placed under cave, broad-leaved is chosen in plantation area, 6 battens are stood in soil pit
Jam-packed forms a timber cylindrical body, periphery soil landfill；To impregnate 500 grams, 500 grams halimasch of broad-leaved more than 3 hours,
500 grams domestication after gram umbellate pore furgus cultivar suitably mix thoroughly, be squeezed into erect timber centre gap in；It is covered on cave with soil,
Form the little Tu Bao that a height is no more than 10 centimetres；The distance between cave and cave are at 10 centimetres or more when plantation；Generally often put down
Square rice can plant 4 caves.
Embodiment 2.
A kind of cultural method of umbellate pore furgus, this method include preparatory technology step, planting operations processing step before planting.
Preparatory technology step includes before the plantation: umbellate pore furgus strain domestication, waste wood processing are planted in vertical stake.
The stake plants the concrete operations of umbellate pore furgus strain domestication and includes: PDA culture medium preparation, the inoculation of nucleome tissue and cultivate.
Prepared by PDA culture medium, for a PDA culture medium.
Weigh 50 grams of sawdusts, 150 g potatos；Potato is switched to thickness less than 5 millimeters, and lateral longitudinal length rains 5 lis
Rice；Sawdust, the potato block cut are added 1000 milliliters of water, 100 degrees Celsius of sealings are boiled 10 minutes, with filtered through gauze, are obtained
The level-one auxiliary liquid of level-one auxiliary liquid, filtering is no less than 1000 milliliters.
10 grams of glucose, 17 grams of agar are added into level-one auxiliary liquid, second level auxiliary liquid are obtained after completely dissolution, by second level auxiliary liquid
It is distributed into test tube, about 5-10 milliliters of every test tube, less than 4 centimetres, second level auxiliary liquid accounts for test tube solvent to be less than test tube opening diameter
20 the percent of test tube total measurement (volume).
It will be put into equipped with second level auxiliary liquid test tube, sterilize 20 minutes in 121 degrees Celsius of environment, and take out test tube, test tube inclination 30
Degree is tiltedly put, and spare, as a PDA culture medium in each test tube after cooling is stored.
The inoculation of nucleome tissue and cultivation.
A PDA culture medium is taken, is placed in the empty culture dish of 10 centimetres of diameter, it aseptically will be fresh
Sclerotia nucleome tissue segmentation at 5 millimeters of square fritters below, every part of PDA culture medium places 3 pieces of sclerotias
Nucleome tissue is set and is cultivated under 23 degrees celsius of room temperature at triangle.
After 28 days, the strain of picking stalwartness mycelia is transferred in the test tube equipped with 1 part of PDA culture medium, and it is Celsius to set room temperature 23
Training is formed until umbellate pore furgus parent species under the conditions of degree, that is, the umbellate pore furgus cultivar after taming.
Waste wood processing.
10 centimetres of waste wood of diameter is sawn into long 55 centimetres of battens, is impregnated 2 days in distilled water, it is dry in ventilation after pulling out
It is placed 8 hours in dry sublimity.
The planting operations technique includes as follows.
Plant the time to choose: in annual 3-5 month, soil temperature can choose soil at 5-15 degrees Celsius and start to cultivate.
Choose in plantation area: cultivation should be selected in height above sea level at 1000-1600 meters, and on 0.5 meter, soil has soil thickness
Machine matter content is between 8% or more, soil pH value 5.5-7.2, and soil year-round average temperature is in 7-15 degrees Celsius of leaf wood woods or woods
On area, soil, periphery.
Cultivation operation: digging about 40 centimetres of diameter or more cylindric soil pits, soil pit depth in soil and be longer than 5 centimetres of batten,
One layer of broad-leaved impregnated in water more than 3 hours is placed under cave, broad-leaved is chosen in plantation area, 4 battens are stood in soil pit
Jam-packed forms a timber cylindrical body, periphery soil landfill；To impregnate 500 grams, 500 grams halimasch of broad-leaved more than 3 hours,
500 grams domestication after gram umbellate pore furgus cultivar suitably mix thoroughly, be squeezed into erect timber centre gap in；It is covered on cave with soil,
Form the little Tu Bao that a height is no more than 10 centimetres；The distance between cave and cave are at 10 centimetres or more when plantation；Generally often put down
Square rice can plant 3 caves.
Embodiment 3.
A kind of cultural method of umbellate pore furgus, this method include preparatory technology step, planting operations processing step before planting.
Preparatory technology step includes before the plantation: umbellate pore furgus strain domestication, waste wood processing are planted in vertical stake.
The stake plants the concrete operations of umbellate pore furgus strain domestication and includes: PDA culture medium preparation, the inoculation of nucleome tissue and cultivate.
Prepared by PDA culture medium, for a PDA culture medium.
Weigh 60 grams of sawdusts, 180 g potatos；Potato is switched to thickness less than 5 millimeters, and lateral longitudinal length rains 5 lis
Rice；Sawdust, the potato block cut are added 1000 milliliters of water, 100 degrees Celsius of sealings are boiled 12 minutes, with filtered through gauze, are obtained
The level-one auxiliary liquid of level-one auxiliary liquid, filtering is no less than 1000 milliliters.
15 grams of glucose, 18 grams of agar are added into level-one auxiliary liquid, second level auxiliary liquid are obtained after completely dissolution, by second level auxiliary liquid
It is distributed into test tube, about 5-10 milliliters of every test tube, less than 4 centimetres, second level auxiliary liquid accounts for test tube solvent to be less than test tube opening diameter
20 the percent of test tube total measurement (volume).
It will be put into equipped with second level auxiliary liquid test tube, sterilize 25 minutes in 121 degrees Celsius of environment, and take out test tube, test tube inclination 35
Degree is tiltedly put, and spare, as a PDA culture medium in each test tube after cooling is stored.
The inoculation of nucleome tissue and cultivation.
A PDA culture medium is taken, is placed in the empty culture dish of 10 centimetres of diameter, it aseptically will be fresh
Sclerotia nucleome tissue segmentation at 5 millimeters of square fritters below, every part of PDA culture medium places 3 pieces of sclerotias
Nucleome tissue is set and is cultivated under 24 degrees celsius of room temperature at triangle.
After 30 days, the strain of picking stalwartness mycelia is transferred in the test tube equipped with 1 part of PDA culture medium, and it is Celsius to set room temperature 24
Training is formed until umbellate pore furgus parent species under the conditions of degree, that is, the umbellate pore furgus cultivar after taming.
Waste wood processing.
15 centimetres of waste wood of diameter is sawn into long 60 centimetres of battens, is impregnated 3 days in distilled water, it is dry in ventilation after pulling out
It is placed 8 hours in dry sublimity.
The planting operations technique includes as follows.
Plant the time to choose: in annual 3-5 month, soil temperature can choose soil at 5-15 degrees Celsius and start to cultivate.
Choose in plantation area: cultivation should be selected in height above sea level at 1000-1600 meters, and on 0.5 meter, soil has soil thickness
Machine matter content is between 8% or more, soil pH value 5.5-7.2, and soil year-round average temperature is in 7-15 degrees Celsius of leaf wood woods or woods
On area, soil, periphery.
Cultivation operation: digging about 40 centimetres of diameter or more cylindric soil pits, soil pit depth in soil and be longer than 7 centimetres of batten,
One layer of broad-leaved impregnated in water more than 3 hours is placed under cave, broad-leaved is chosen in plantation area, 5 battens are stood in soil pit
Jam-packed forms a timber cylindrical body, periphery soil landfill；To impregnate 500 grams, 500 grams halimasch of broad-leaved more than 3 hours,
500 grams domestication after gram umbellate pore furgus cultivar suitably mix thoroughly, be squeezed into erect timber centre gap in；It is covered on cave with soil,
Form the little Tu Bao that a height is no more than 10 centimetres；The distance between cave and cave are at 10 centimetres or more when plantation；Generally often put down
Square rice can plant 4 caves.
Claims (1)
1. a kind of cultural method of umbellate pore furgus, this method includes preparatory technology step, planting operations processing step before planting；
Preparatory technology step includes before the plantation: umbellate pore furgus strain domestication, waste wood processing are planted in vertical stake；
The stake plants the concrete operations of umbellate pore furgus strain domestication and includes: PDA culture medium preparation, the inoculation of nucleome tissue and cultivate；
Prepared by PDA culture medium, for a PDA culture medium；
Weigh 50-70 grams of sawdust, 150-200 g potato；Potato is switched to thickness less than 5 millimeters, and lateral longitudinal length rains 5
Centimetre；Sawdust, the potato block cut are added 1000 milliliters of water, 100 degrees Celsius of sealings are boiled 10-15 minutes, with filtered through gauze,
Level-one auxiliary liquid is obtained, the level-one auxiliary liquid of filtering is no less than 1000 milliliters；
10-20 grams of glucose, 17-20 grams of agar are added into level-one auxiliary liquid, obtains second level auxiliary liquid after completely dissolution, second level is auxiliary
Liquid is distributed into test tube, and about 5-10 milliliters of every test tube, for test tube opening diameter less than 4 centimetres, second level auxiliary liquid accounts for test tube solvent cannot be small
In 20 the percent of test tube total measurement (volume)；
It will be put into equipped with second level auxiliary liquid test tube, sterilize 20-30 minutes in 121 degrees Celsius of environment, and take out test tube, test tube tilts 30-
40 degree tiltedly put, and store spare, as a PDA culture medium in each test tube after cooling；
The inoculation of nucleome tissue and cultivation；
A PDA culture medium is taken, is placed in the empty culture dish of 10 centimetres of diameter, aseptically by fresh pig
The nucleome tissue segmentation of Siberian cocklebur sclerotium places the nucleome of 3 pieces of sclerotias at 5 millimeters of square fritters below, every part of PDA culture medium
Tissue, at triangle, sets and cultivates under room temperature 23-26 degrees celsius；
After 28-33 days, the strain of picking stalwartness mycelia is transferred in the test tube equipped with 1 part of PDA culture medium, is set room temperature 23-26 and is taken the photograph
Training is formed until umbellate pore furgus parent species under the conditions of family name's degree, that is, the umbellate pore furgus cultivar after taming；
Waste wood processing；
10-20 centimetres of diameter of waste wood is sawn into long 55-65 centimetres of batten, is impregnated 2-3 days in distilled water, logical after pulling out
It air-dries in dry sublimity and places 8-12 hours；
The planting operations technique includes as follows；
Plant the time to choose: in annual 3-5 month, soil temperature can choose soil at 5-15 degrees Celsius and start to cultivate；
Choose in plantation area: cultivation should be selected in height above sea level at 1000-1600 meters, and soil thickness is on 0.5 meter, the organic matter of soil
Content is between 8% or more, soil pH value 5.5-7.2, and soil year-round average temperature is in 7-15 degrees Celsius of leaf wood woods or forest zone week
On the soil of side；
Cultivation operation: about 40 centimetres of diameter or more cylindric soil pits are dug in soil, soil pit depth is longer than 5-8 centimetres of batten, in cave
Lower to place one layer of broad-leaved impregnated in water more than 3 hours, broad-leaved is chosen in plantation area, 4-6 root batten is stood in soil pit
Jam-packed forms a timber cylindrical body, periphery soil landfill；To impregnate 500 grams, 500 grams halimasch of broad-leaved more than 3 hours,
500 grams domestication after gram umbellate pore furgus cultivar suitably mix thoroughly, be squeezed into erect timber centre gap in；It is covered on cave with soil,
Form the little Tu Bao that a height is no more than 10 centimetres；The distance between cave and cave are at 10 centimetres or more when plantation；Generally often put down
Square rice can plant the cave 3-4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811630065.9A CN109496685A (en) | 2018-12-28 | 2018-12-28 | A kind of cultural method of umbellate pore furgus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201811630065.9A CN109496685A (en) | 2018-12-28 | 2018-12-28 | A kind of cultural method of umbellate pore furgus |
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| CN109496685A true CN109496685A (en) | 2019-03-22 |
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| CN201811630065.9A Pending CN109496685A (en) | 2018-12-28 | 2018-12-28 | A kind of cultural method of umbellate pore furgus |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1521256A (en) * | 2003-02-12 | 2004-08-18 | 刘德印 | Polyporus umbellatus |
| CN1918964A (en) * | 2005-08-26 | 2007-02-28 | 万源市大巴山生物科技开发有限公司 | Polyporus umbellatus artificial cultivation method |
| CN102037853A (en) * | 2009-10-21 | 2011-05-04 | 辽宁省林业科学研究院 | Artificially cultured Grifulaumbelleta strain, Amillariella mellea and sclerotium production method for Grifulaumbelleta strain |
| CN104718983A (en) * | 2015-03-07 | 2015-06-24 | 甘肃天水岐黄药业有限责任公司 | Strain acclimatizing method for artificial grifola cultivation |
-
2018
- 2018-12-28 CN CN201811630065.9A patent/CN109496685A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1521256A (en) * | 2003-02-12 | 2004-08-18 | 刘德印 | Polyporus umbellatus |
| CN1918964A (en) * | 2005-08-26 | 2007-02-28 | 万源市大巴山生物科技开发有限公司 | Polyporus umbellatus artificial cultivation method |
| CN102037853A (en) * | 2009-10-21 | 2011-05-04 | 辽宁省林业科学研究院 | Artificially cultured Grifulaumbelleta strain, Amillariella mellea and sclerotium production method for Grifulaumbelleta strain |
| CN104718983A (en) * | 2015-03-07 | 2015-06-24 | 甘肃天水岐黄药业有限责任公司 | Strain acclimatizing method for artificial grifola cultivation |
Non-Patent Citations (1)
| Title |
|---|
| 陇南康县三河坝镇: "三河坝乡贾仲勇（竖式桩栽法）栽培猪苓喜获丰收" * |
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