CN109486896A - 一种催化拆分制备异甘草酸的方法 - Google Patents
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Abstract
本发明公开一种催化拆分制备异甘草酸的方法,包括:将脂肪酶与消旋的甘草酸甲酯加入到有机溶剂和水的混合溶剂中,搅拌进行酶促反应,反应后于水相中得到18‑α甘草酸,于有机相中得到18‑β甘草酸甲酯。使用该方法,不仅可以获得高光学纯度的单一对映体18‑α甘草酸,而且与传统的化学拆分工艺相比,可以减轻生产过程的劳动强度和环境污染,并降低生产成本,为生物法制备18‑α甘草酸提供了新的途径。
Description
技术领域
本发明属于药物化学领域,具体涉及一种催化拆分制备异甘草酸的方法。
背景技术
甘草制剂是临床一线常用的重大抗肝炎药物品种。现代研究已证实天然甘草中最主要的活性物质是甘草酸,自然界中甘草酸存在18α-、18β-两种构型,天然甘草中以18β-甘草酸为主(18α-甘草酸含量低于5%)。18α-甘草酸(异甘草酸)的结构式为:18β-甘草酸(甘草酸)的结构式为两种构型的甘草酸通过作用于激素受体,影响离子通道(抑制钙离子),激活或抑制酶的活性,调节物质代谢,调节胆碱能神经的兴奋性,具有肾上腺皮质激素样作用,呈现明显的抗炎和免疫调节效应。
异甘草酸的亲脂性大于甘草酸,在体内更易与受体蛋白和类固醇激素的靶细胞受体结合而发挥抗炎、保护肝细胞膜及改善肝功能的作用。我国首创的手性药物异甘草酸镁注射剂成功研发上市。异甘草酸镁是以天然来源的18β-甘草酸为原料,经过消旋差向异构化、化学拆分得到单一手性18α-异构体、成盐结晶等工艺步骤得到。目前异甘草酸镁注射剂在急慢性肝炎、药物性肝损伤等临床应用上显示出独特优势,取得了巨大成功,2012年该品种国内销售已经超过11.9亿元。
现有的手性异甘草酸制备工艺为化学拆分法,即将消旋的甘草酸,在乙酰氯催化下,与甲醇反应,生成消旋的甘草酸甲酯,然后利用18α-异构体甲酯的溶解度比18β-异构体甲酯的溶解度低,故18α-异构体甲酯可优先沉淀析出。
该工艺存在重大的缺陷,两者异构体甲酯的溶解度差别太小,且异构体甲酯属于两亲化合物难以结晶,故产物的手性纯度低,往往需要反复沉淀析出几十次,才能得到手性纯度合格的产物,工艺步骤多、收率差,同时工艺过程使用到有毒试剂乙酰氯,原子经济性太差,三废严重。
发明内容
针对上述现有技术的问题,本发明公开一种利用脂肪酶催化拆分制备异甘草酸的方法,该方法能够解决现有以化学拆分法制备异甘草酸过程中步骤繁多、手性纯度低、原子利用率不高,三废严重的问题。
为实现上述目的,本发明采用如下的技术方案:
一种催化拆分制备异甘草酸的方法,包括如下步骤:
将脂肪酶与消旋的甘草酸甲酯加入到有机溶剂和水的混合溶剂中,搅拌进行酶促反应,反应后于水相中得到18-α甘草酸,于有机相中得到18-β甘草酸甲酯。
进一步的,所述消旋的甘草酸甲酯为18α-甘草酸甲酯与18-β甘草酸甲酯的混合物。
进一步的,所述有机溶剂为正己烷、正庚烷、正辛烷或异辛烷中的一种或几种。
进一步的,所述脂肪酶为产碱假单胞菌(Pseudomonas alcaligenes NERCB-LU9844)脂肪酶。
进一步的,所述酶促反应的温度为20~50℃。
进一步的,所述酶促反应的时间为15~30h。
进一步的,所述脂肪酶的用量为10~30mg/mL,优选25mg/mL。
进一步的,所述混合溶剂中,水与有机溶剂的体积比为1:10~20。
进一步的,所述酶促反应的水相PH值为8~10。
进一步的,所述酶促反应的水相中采用磷酸二氢钠或磷酸二氢钾作为缓冲剂。
脂肪酶催化的酯水解反应,与化学催化(酸或碱)的酯水解反应相比具有许多独特优势,在酯键的立体选择性水解(定向水解)方面具有优势,可以完成常规化学催化水解反应无法完成的任务。本发明以生物催化拆分工艺替代化学拆分工艺,以18α-甘草酸酯和18β-甘草酸酯为底物,加入筛选的特定脂肪酶,在微水有机相中选择性水解18α-异构体酯,可得到高手性纯度的18α-甘草酸。
具体实施方式
反应底物及产物定性定量检测方式为:色谱柱:Durashell C18(4.6×250mm,5μm)流动相:V(甲醇):V(水):V(冰醋酸)=40:60:0.5(氨水调节pH 7.2);流速:1.0mL/min;检测波长:250nm;柱温:25℃;进样量:10uL。
实施例1
在25mL加塞磨口锥形瓶中将18-α甘草酸甲酯、18-β甘草酸甲酯的混合物(5g)和产碱假单胞菌脂肪酶加入到共10mL的水:正己烷=0.5:9.5(v/v)的混合溶剂中,产碱假单胞菌脂肪酶用量为25mg/mL,20℃条件下以170r/min,在摇床中反应15h,反应后于水相中得到18-α甘草酸,转化率达27%,18-α甘草酸的光学纯度=99%ee。
实施例2
在25mL加塞磨口锥形瓶中将18-α甘草酸甲酯、18-β甘草酸甲酯混合物(5g)和产碱假单胞菌脂肪酶加入到共11mL的水:正庚烷=1:10(v/v)的混合溶剂中,产碱假单胞菌脂肪酶用量为10mg/mL,30℃条件下以170r/min,在摇床中反应20h,反应后于水相中得到18-α甘草酸,转化率达35%,18-α甘草酸的光学纯度=99.1%ee。
实施例3
在25mL加塞磨口锥形瓶中将18-α甘草酸甲酯、18-β甘草酸甲酯混合物(5g)和产碱假单胞菌脂肪酶加入到共10.5mL的水:正庚烷=0.5:10(v/v)的混合溶剂中,产碱假单胞菌脂肪酶用量为20mg/mL,40℃条件下以170r/min,在摇床中反应25h,反应后于水相中得到18-α甘草酸,转化率达35.5%,18-α甘草酸的光学纯度=99%ee。
实施例4
在25mL加塞磨口锥形瓶中将18-α甘草酸甲酯、18-β甘草酸甲酯混合物(5g)和产碱假单胞菌脂肪酶加入到共10mL的水:正庚烷=0.5:9.5(v/v)的混合溶剂中,产碱假单胞菌脂肪酶用量为30mg/mL,50℃条件下以170r/min,在摇床中反应30h,反应后于水相中得到18-α甘草酸,转化率达30%,18-α甘草酸的光学纯度=98.9%ee。
实施例5
在25mL加塞磨口锥形瓶中将18-α甘草酸甲酯、18-β甘草酸甲酯混合物(5g)和产碱假单胞菌脂肪酶加入到共10mL的水:正辛烷=0.5:9.5(v/v)的混合溶剂中,产碱假单胞菌脂肪酶用量为25mg/mL,30℃条件下以170r/min,在摇床中反应30h,反应后于水相中得到18-α甘草酸,转化率达37%,18-α甘草酸的光学纯度=99.2%ee。
实施例6
在25mL加塞磨口锥形瓶中将18-α甘草酸甲酯、18-β甘草酸甲酯混合物(5g)和产碱假单胞菌脂肪酶加入到共10mL的水:异辛烷=0.5:9.5(v/v)的混合溶剂中,产碱假单胞菌脂肪酶用量为25mg/mL,30℃条件下以170r/min,在摇床中反应30h,反应后于水相中得到18-α甘草酸,转化率达34%,18-α甘草酸的光学纯度=99%ee。
Claims (10)
1.一种催化拆分制备异甘草酸的方法,其特征在于,包括如下步骤:
将脂肪酶与消旋的甘草酸甲酯加入到有机溶剂和水的混合溶剂中,搅拌进行酶促反应,反应后于水相中得到18-α甘草酸,于有机相中得到18-β甘草酸甲酯。
2.根据权利要求1所述的方法,其特征在于,所述消旋的甘草酸甲酯为18α-甘草酸甲酯与18-β甘草酸甲酯的混合物。
3.根据权利要求1所述的方法,其特征在于,所述有机溶剂为正己烷、正庚烷、正辛烷或异辛烷中的一种或几种。
4.根据权利要求1所述的方法,其特征在于,所述脂肪酶为产碱假单胞菌脂肪酶。
5.根据权利要求1所述的方法,其特征在于,所述酶促反应的温度为20~50℃。
6.根据权利要求1所述的方法,其特征在于,所述酶促反应的时间为15~30h。
7.根据权利要求1所述的方法,其特征在于,所述脂肪酶的用量为10~30mg/mL,优选25mg/mL。
8.根据权利要求1所述的方法,其特征在于,所述混合溶剂中,水与有机溶剂的体积比为1:10~20。
9.根据权利要求1所述的方法,其特征在于,所述酶促反应的PH值为8~10。
10.根据权利要求1所述的方法,其特征在于,包括如下步骤:
将产碱假单胞菌脂肪酶与18α-甘草酸甲酯与18-β甘草酸甲酯的混合物加入到有机溶剂和水的混合溶剂中,搅拌,于20~50℃进行酶促反应15~30h,脂肪酶的用量为10~30mg/mL,反应后于水相中得到18-α甘草酸,于有机相中得到18-β甘草酸甲酯。
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