CN109486740B - Preparation method and application of semi-dry nitrobacter - Google Patents
Preparation method and application of semi-dry nitrobacter Download PDFInfo
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Abstract
The invention belongs to the technical field of biology and water treatment, and provides a preparation method and application of a semi-dry nitrobacter agent, wherein the preparation method comprises the following specific steps: (1) taking the activated sludge of an aerobic tank of a sewage treatment plant which normally runs as a bacteria source, and adding the activated sludge into an inorganic salt culture medium special for nitrobacteria for enrichment culture; (2) carrying out acclimatization culture after enrichment culture to obtain a culture solution with high nitrification activity; (3) concentrating the culture solution, and culturing in a protective solution with a certain concentration for a proper time; (4) uniformly mixing the mixed solution obtained in the step (3) with the treated carrier to obtain a microbial inoculum; (5) the microbial inoculum is filled into a vacuum sealing bag, vacuumized and sealed, and placed in a shade place for normal temperature storage. When the microbial inoculum is used, the microbial inoculum is only dispersed by water and then is added into an aerobic biochemical system, so that the microbial inoculum has the characteristics of high activity, high tolerance, good stability, strong stress resistance and the like, and has the advantages of convenience in transportation and storage, small occupied space and the like; it can be applied without activation and rejuvenation.
Description
Technical Field
The invention belongs to the technical field of biology and water treatment, and provides a preparation method and application of a semi-dry nitrobacter agent.
Background
Ammonia nitrogen has become one of the ubiquitous pollutants in water environments, and harms the safety of aquatic organisms and even the entire ecosystem. The denitrification comprises a physical method, a chemical method and a biological method, and the biological method has the advantages of simple operation, low investment, low cost, no secondary pollution, good treatment effect and the like, so the method is widely applied to sewage treatment at present. Nitrifying bacteria are microorganisms playing a main role in the biological denitrification process, and the stability of nitrification and the improvement of nitrification rate are the keys influencing the denitrification efficiency of the whole system. Nitrifying bacteria are strict aerobic autotrophic bacteria, have strict living conditions and slow growth, and are often impacted in a sewage treatment biochemical system with frequently fluctuating water quality and quantity to cause the collapse of a nitrifying system. The method has important significance for enhancing the biological denitrification efficiency by adding dominant strains into the biochemical system to enhance the biological nitrification process, and is an important way for enhancing the nitrification function of the biochemical system commonly used in the current sewage treatment process.
However, how to obtain a high-efficiency nitrifying bacteria agent with good nitrification effect, strong tolerance, strong stress resistance and lower cost is the key for solving the problems. In addition, the liquid microbial inoculum is large in volume, wide in occupied space and inconvenient to transport and store, how to make the nitrifying microbial inoculum into a solid state or a semi-solid state and store the nitrifying microbial inoculum for a long time and the like, and the problem to be solved in the denitrification research is urgent.
Disclosure of Invention
Aiming at the defects of the technology, the invention provides a preparation method and application of a semi-dry nitrifying bacteria agent, and the preparation method specifically comprises the following steps: (1) taking the activated sludge of an aerobic tank of a sewage treatment plant which normally runs as a bacteria source, and adding the activated sludge into an inorganic salt culture medium special for nitrobacteria for enrichment culture; (2) carrying out acclimatization culture after enrichment culture to obtain a culture solution with high nitrification activity; (3) concentrating the culture solution, and culturing in a protective solution with a certain concentration for a proper time; (4) uniformly mixing the mixed solution obtained in the step (3) with the treated carrier to obtain a microbial inoculum; (5) the microbial inoculum is filled into a vacuum sealing bag, vacuumized and sealed, and placed in a shade place for normal temperature storage. When the microbial inoculum is used, the microbial inoculum is dispersed by water and then added into an aerobic biochemical system. The prepared nitrifying bacteria agent is in a semi-dry solid state, has the characteristics of high activity, high tolerance, good stability, strong stress resistance and the like, and has the advantages of incomparable convenience in transportation and storage, small occupied space and the like compared with liquid bacteria agents; and the activity is more than 90% after the storage for one year, and the activation and rejuvenation are not needed during application.
The specific technical scheme of the invention is as follows:
a preparation method of a semi-dry nitrobacter agent comprises the following steps:
(1) taking activated sludge in an aerobic tank of a sewage treatment plant which normally runs as a bacteria source, and adding the activated sludge into an inorganic salt culture medium special for nitrobacteria for enrichment culture;
(2) carrying out acclimatization culture after enrichment culture to obtain a culture solution with high nitrification activity;
(3) concentrating the culture solution, and culturing in a protective solution with a certain concentration for a proper time;
(4) and (4) uniformly mixing the mixed solution obtained in the step (3) with the treated carrier to obtain the microbial inoculum.
Further, the method comprises the following specific steps:
(1) selecting activated sludge in an aerobic tank of a sewage treatment plant which normally runs, standing for 1-3 hours, and taking the supernatant as a bacteria source after discarding;
the inventor does not need to describe the sewage treatment plant which can stably run after debugging, and the invention aims to enrich and domesticate the conventional aerobic tank activated sludge and improve the nitrification capacity of the conventional aerobic tank activated sludge, so that the aerobic tank activated sludge in each sewage treatment plant can be correspondingly domesticated and improved in the nitrification capacity;
(2) preparing an inorganic salt culture medium special for nitrobacteria, wherein the formula is as follows: 1.0-2.0g/L of sodium bicarbonate, 1.0-2.0g/L of dipotassium phosphate trihydrate, 1.0-2.0g/L of potassium dihydrogen phosphate, 0.1-0.5g/L of sodium chloride, 0.02-0.1g/L of ferrous sulfate heptahydrate, 0.02-0.1g/L of anhydrous magnesium sulfate, 0.01-0.1g/L of copper sulfate, 0.5-3.0g/L of ammonium sulfate and water as a solvent.
Compared with the culture medium commonly used in the prior art, the culture medium adopted by the invention is mainly characterized in that an inorganic salt culture medium, particularly a carbon source (sodium bicarbonate) and a nitrogen source (ammonium sulfate) are completely adopted, and the microbial inoculum adopted by the invention is autotrophic bacteria, and the inorganic carbon source can be utilized to synthesize the self-requirement, so that the culture medium containing organic substances is not required to be adopted like the prior art, the cost is reduced, the quality problem of the culture medium caused by deterioration of organic substances and the like can be avoided, meanwhile, the ammonium sulfate is added into the culture medium to improve the ammonia nitrogen degradation capability of the microbial inoculum, and the salt tolerance of the microbial inoculum can be domesticated by adding high-salt components.
(3) And (2) adding the bacteria source treated in the step (1) into the inorganic culture medium special for nitrobacteria, and uniformly stirring, wherein the weight ratio of the bacteria source to the inorganic salt culture medium special for nitrobacteria is 1:9-4: 6.
(4) Carrying out enrichment culture, wherein the enrichment culture method comprises the following steps: aerating the culture medium by using an electromagnetic oxygenation pump, and maintaining the dissolved oxygen at 2.0-6.0 mg/L; the temperature is 20-35 deg.C, the culture medium is changed every day, ammonium sulfate is used to supplement the ammonia nitrogen of the culture medium to reach concentration of 50-150mg/L, pH is kept at 6.5-8.5, and the enrichment culture time is 5-7 days.
(5) Carrying out domestication culture, wherein the domestication culture method comprises the following steps: aerating the culture medium by using an electromagnetic oxygenation pump, and maintaining the dissolved oxygen at 2.0-6.0 mg/L; the temperature is 20-35 ℃, the culture medium is replaced for 2-4 days after culture, ammonium sulfate is used for supplementing ammonia nitrogen concentration to 250mg/L, sodium chloride is used for supplementing sodium chloride to ensure that the total salt of the culture medium reaches 10000mg/L, and the pH value is kept at 6.5-8.5; then replacing the culture medium every 2-4 days, and in addition, supplementing ammonium sulfate to increase the ammonia nitrogen concentration by 100mg/L on the basis of the last time and supplementing sodium chloride to increase the total salt concentration by 10000mg/L on the basis of the last time every 4-7 days of culture until the ammonia nitrogen concentration of the culture medium is increased to 550mg/L and the total salt is increased to 40000 mg/L; the acclimation time is 20-35 days.
After the acclimatization is finished, the nitrifying activity of the culture solution reaches more than 50 mg/(L.h).
The method for replacing the culture medium can adopt a mode of removing the liquid culture medium in a centrifugal mode and then adding a new culture medium, or adopts a mode of standing to precipitate the mixed solution and directly adding the new culture medium after removing the supernatant, and the inventor does not need to describe again;
(6) concentrating the culture solution with high nitrification activity, wherein the concentration method comprises the following steps: centrifuging at 2000-;
(7) preparing a protective solution,
when the protective solution is sodium nitrite solution, the concentration of the protective solution is 5mg/L-50mg/L, and the mass ratio of the concentrated thalli to the protective solution is 1:1-1: 8;
when the protective solution is trehalose solution or nitrobacteria inorganic salt culture medium solution, the concentration of the protective solution is 200mg/L-1000 mg/L; and the mass ratio of the concentrated thalli to the protective solution is 1:3-1: 9;
the culture time of the concentrated thalli in the protective solution is 10min-2 h; the weight percentage of the water in the mixed solution of the concentrated thalli and the protective solution is detected at the same time and recorded as W 1 。
(8) The mass ratio of the mixed solution of the concentrated thallus and the protective solution to the carrier is recorded as X: Y, and the condition W is satisfied 1 *X＝(10％-50％)*(X+Y)；
10% -50% of the above formula is the moisture content range in the semi-dry state in the field, and those skilled in the art can select the corresponding percentage in the above range according to the needs to calculate conveniently;
the carrier is one or more of diatomite, kaolin, zeolite powder or bentonite, and is cheap and easy to obtain, so that the production cost of the microbial inoculum is low, the carrier is treated by a damp-heat sterilization method, and the sterilization is carried out for 15-30min at 115 ℃; and (4) uniformly mixing the mixed solution obtained in the step (7) with the treated carrier to obtain the microbial inoculum, wherein the water content is 10-50%.
(9) The microbial inoculum is filled into a vacuum sealing bag, vacuumized and sealed, and placed in a shade place for normal temperature storage.
The nitrification rate of the finally obtained nitrifying bacteria agent is more than 500mg/(L.d), the nitrifying bacteria agent is in a semi-dry solid state, the water content is 10-50%, and the semi-dry solid bacteria agent overcomes the defects of large volume, difficult transportation, difficult storage and the like of a liquid bacteria agent; the nitrifying bacteria agent can tolerate full salt concentration of 1000-40000mg/L and has the characteristic of high tolerance, and the normal activity of the bacteria agent can be ensured within the tolerance range. Experiments prove that the nitrification activity can still be kept above 90 percent of the original microbial inoculum after 12 months of storage, and the activation or rejuvenation is not needed during application.
The semi-dry solid microbial inoculum obtained by the invention is obtained by taking the activated sludge of an aerobic pool of an operating sewage treatment plant as a raw material, wherein the activated sludge of the aerobic pool is different according to the properties of the treated sewage, the conditions of local strains and the like.
The nitrification rate of the bacterial agent obtained by the method is more than 500mg/(L.d), can tolerate 100-1000mg/L ammonia nitrogen concentration and 1000-40000mg/L salinity, and has the characteristics of high activity and high tolerance; the microbial inoculum keeps the complete microbial components in the nitrification system and has the advantages of good stability, strong stress resistance and the like; after the microbial inoculum is stored for one year, the activity of the microbial inoculum still reaches more than 90 percent, and the activation and rejuvenation are not needed during application; compared with the primary sludge microbial inoculum, the method has more pertinence to the degradation of ammonia nitrogen, and the nitrification activity is improved by more than 5-10 times compared with the primary sludge; meanwhile, the defects of large occupied space, inconvenient transportation and storage and the like of the liquid microbial inoculum are overcome.
The obtained microbial inoculum is semi-dry, is convenient to store and transport, and can effectively improve the nitrification function by only dispersing the microbial inoculum with water and adding the microbial inoculum into an aerobic biochemical system according to a proper proportion when in use.
The semi-dry nitrifying bacteria agent can be applied to sewage treatment in the industries of agriculture, petrifaction, leather and the like, and the adding proportion is 0.05 per mill-0.5 per mill of the volume of an aerobic system of a biochemical system, so that the nitrification function of the biochemical system can be effectively improved: the nitrification rate is improved by 10-40%, the ammonia nitrogen index of the sewage inlet is improved by 5-20%, and the system tolerance is obviously improved.
The adding proportion of the conventional liquid microbial inoculum adopted in the prior art in an aerobic system of a biochemical system is 1-10 per mill of the volume, so that the microbial inoculum has better effect, the adding amount is lower, the treatment cost is reduced, and the obvious progress is achieved;
in conclusion, the nitrifying bacteria agent prepared by the invention is in a semi-dry solid state, has the characteristics of high activity, high tolerance, good stability, strong stress resistance and the like, and has the advantages of incomparable convenience for transportation and storage, small occupied space and the like compared with liquid bacteria agents; and the activity is more than 90% after the storage for one year, and the activation and rejuvenation are not needed during application.
Detailed Description
The present invention will be described in further detail with reference to the following examples, but it should not be construed that the scope of the present invention is limited to the examples. All the technologies realized based on the above contents of the present invention belong to the scope of the present invention, and the following embodiments are all completed by adopting the conventional prior art except for the specific description.
Example 1
Preparing a semi-dry nitrobacter agent, taking 20L of activated sludge in an aerobic tank of a sewage treatment plant which normally operates, standing for 2h, discarding supernatant, centrifuging the precipitated activated sludge at the rotating speed of 2000r/min for 2min, discarding supernatant, and obtaining concentrated activated sludge with the volume of 1L;
preparing 9L of inorganic salt culture medium special for nitrobacteria, wherein the formula is as follows: 1.0g/L of sodium bicarbonate, 1.0g/L of dipotassium hydrogen phosphate trihydrate, 1.0g/L of potassium dihydrogen phosphate, 0.1g/L of sodium chloride, 0.02g/L of ferrous sulfate heptahydrate, 0.02g/L of anhydrous magnesium sulfate, 0.02g/L of copper sulfate, 0.5g/L of ammonium sulfate and the balance of water.
Adding the concentrated activated sludge into a culture medium, and starting enrichment culture work of nitrobacteria. The enrichment culture method comprises the following steps: aerating the 10L culture medium by using an electromagnetic oxygenation pump, and maintaining dissolved oxygen at 3.0 mg/L; the temperature is 25 ℃, the culture medium is replaced every day, meanwhile, ammonium sulfate is used for supplementing the ammonia nitrogen concentration of the culture medium to 70mg/L, the pH value is kept at 7.0, and the enrichment culture time is 7 days.
After the enrichment culture is finished, performing acclimatization culture, wherein the acclimatization culture method comprises the following steps: aerating the culture medium by using an electromagnetic oxygenation pump, and maintaining 4.0mg/L of dissolved oxygen; the temperature is 30 ℃, the culture medium is replaced once every 2 days, the ammonia nitrogen concentration is 250mg/L, sodium chloride is supplemented to ensure that the total salt content of the culture medium is 10000mg/L, the pH value is kept at 8.0, after the culture is carried out for 7 days, in addition to replacing the culture medium according to the standard, the ammonia nitrogen concentration is increased by 100mg/L on the basis of the previous time by using ammonium sulfate every 7 days, the sodium chloride is supplemented to increase the total salt concentration by 10000mg/L on the basis of the previous time until the ammonia nitrogen concentration of the culture medium is increased to 550mg/L, and the total salt concentration is increased to 40000 mg/L. The acclimation time is 28 days; after the acclimation is finished, the nitrifying activity of the culture solution reaches more than 50 mg/(L.h).
Centrifuging the culture solution at 2000r/min for 2min, and discarding the supernatant to obtain 0.8kg of concentrated thallus;
preparing 2 wt% sodium nitrite solution as protective solution, mixing concentrated thallus with protective solution at a weight ratio of 1:2 (2.4 kg (marked as X), culturing in shaking table for 10min, and detecting water content of mixed solution of concentrated thallus and protective solution (marked as W) of 80% 1 )。
Selecting kaolin as a carrier, wherein the weight of the carrier is Y, and obtaining the kaolin through a formula W 1 The carrier weight was calculated to be 7.2kg by substituting the data 80% × 2.4% × 20% × (2.4+ Y). Sterilizing the carrier at 115 deg.C for 20 min; mixing the mixed solution with the carrier, packaging into vacuum sealing bag, vacuumizing, sealing, and storing at room temperature in shade.
Example 2
Preparing a semi-dry nitrobacteria agent, taking 50L of activated sludge in an aerobic tank of a sewage treatment plant which normally operates, standing for 2 hours, discarding supernatant, centrifuging the precipitated activated sludge at the rotating speed of 3000r/min for 3min, discarding supernatant, and obtaining concentrated activated sludge with the volume of 3L;
preparing 7L of inorganic salt culture medium special for nitrobacteria, wherein the formula is as follows: 1.2g/L of sodium bicarbonate, 1.5g/L of dipotassium phosphate trihydrate, 1.3g/L of potassium dihydrogen phosphate, 0.4g/L of sodium chloride, 0.02g/L of ferrous sulfate heptahydrate, 0.04g/L of anhydrous magnesium sulfate, 0.02g/L of copper sulfate and 2.0g/L of ammonium sulfate.
Adding the concentrated activated sludge into a culture medium, and starting enrichment culture work of nitrobacteria. The enrichment culture method comprises the following steps: aerating the 10L culture medium by using an electromagnetic oxygenation pump, and maintaining 4.0mg/L dissolved oxygen; the temperature is 28 ℃, the culture medium is replaced every day, the ammonia nitrogen of the supplementary culture medium is 100mg/L, the pH is kept at 7.0, and the enrichment culture time is 6 days.
Carrying out domestication culture after the enrichment culture is finished, wherein the domestication culture method comprises the following steps: aerating the culture medium by using an electromagnetic oxygenation pump, and maintaining 4.0mg/L of dissolved oxygen; the temperature is 28 ℃, the culture medium is replaced once every 2 days, the ammonia nitrogen concentration is 250mg/L, sodium chloride is supplemented to ensure that the total salt content of the culture medium is 10000mg/L, the pH value is kept at 7.5, after the culture is performed for 6 days, besides the culture medium is replaced according to the standard, ammonium sulfate is supplemented every 6 days to increase the ammonia nitrogen concentration by 100mg/L on the basis of the previous time, sodium chloride is supplemented to increase the total salt concentration by 10000mg/L on the basis of the previous time until the ammonia nitrogen concentration of the culture medium is increased to 550mg/L, and the total salt is increased to 40000 mg/L. The acclimation time is 24 days. After the acclimatization is finished, the nitrifying activity of the culture solution reaches more than 50 mg/(L.h).
Centrifuging the culture solution at 4000r/min for 1min, and discarding the supernatant to obtain 1.3kg of concentrated thallus.
Preparing inorganic salt culture medium as protective solution for nitrobacteria, mixing concentrated thallus with protective solution at a ratio of 1:5, culturing 6.5kg (marked as X) in shaking table for 10min, and detecting water content of the mixed solution to 90% (marked as W) 1 )。
Selecting zeolite powder as carrier, the weight of carrier is Y, and making it pass through formula W 1 The carrier weight was calculated to be 8.125kg by substituting data 90% × 6.5% × 40% (6.5+ Y). The vector was sterilized at 115 ℃ for 15 min.
Mixing the mixed solution with the carrier, packaging into vacuum sealing bag, vacuum sealing, and storing in shade at room temperature.
Example 3:
the application of the semi-dry nitrobacter is that the nitrobacter prepared in the embodiment 1 and the embodiment 2 is stored for 12 months and then taken out, and the nitrification effect of the nitrobacter is verified by taking a certain tannery sewage as a treatment object. Respectively establishing 3 aerobic systems, respectively adding 10L of tannery sewage, aerating by using an electromagnetic oxygenation pump, and maintaining 4.0-6.0mg/L of dissolved oxygen; the pH is maintained at 7.5-8.0.
Detecting that the initial ammonia nitrogen concentration of sewage is 275mg/L, the system temperature is 28 ℃, and the adding amount of the microbial inoculum is calculated according to 0.1 per mill, namely 1.0g of each microbial inoculum is weighed, and the microbial inoculum is put into 100mL of tap water for shaking table oscillation for 20min for dispersion, and then the microbial inoculum is added into the 10L sewage system; the commercially available microbial inoculum adopts commercially available conventional solid microbial inoculum (containing nitrifying microbial inoculum, nitrosification microbial inoculum, enzyme preparation, nutritive salt and the like);
the ammonia nitrogen concentration in the sewage is detected every 8 hours, and the data is recorded in the table 1, and the result shows that the prepared semi-dry nitrifying bacteria agent has a good nitrifying function, the ammonia nitrogen removal rate reaches 100%, and the average ammonia nitrogen degradation rate is above 130 mg/(L.d).
Table 1 application data of semi-dry nitrobacteria prepared in example 1 and example 2
The above description is only a preferred embodiment of the present invention, and therefore should not be taken as limiting the scope of the invention, and all equivalent variations and modifications made within the claims and the specification of the present invention should be included in the scope of the present invention.
Claims (5)
1. A preparation method of a semi-dry nitrobacteria agent is characterized by comprising the following steps: the method comprises the following steps:
(1) taking activated sludge in an aerobic tank of a sewage treatment plant which normally runs as a bacteria source, and adding the activated sludge into an inorganic salt culture medium special for nitrobacteria for enrichment culture;
(2) carrying out acclimatization culture after enrichment culture to obtain a culture solution with high nitrification activity;
(3) concentrating the culture solution, and culturing in a protective solution with a certain concentration for a proper time;
(4) uniformly mixing the mixed solution obtained in the step (3) with the treated carrier to obtain a microbial inoculum;
the formula of the inorganic salt culture medium special for the nitrobacteria in the step (1) is as follows: 1.0-2.0g/L of sodium bicarbonate, 1.0-2.0g/L of dipotassium phosphate trihydrate, 1.0-2.0g/L of monopotassium phosphate, 0.1-0.5g/L of sodium chloride, 0.02-0.1g/L of ferrous sulfate heptahydrate, 0.02-0.1g/L of anhydrous magnesium sulfate, 0.01-0.1g/L of copper sulfate, 0.5-3.0g/L of ammonium sulfate and water as a solvent;
the domestication culture method in the step (2) comprises the following steps: aerating the culture medium by using an electromagnetic oxygenation pump, and maintaining the dissolved oxygen at 2.0-6.0 mg/L; the temperature is 20-35 ℃, the culture medium is replaced for 2-4 days, ammonium sulfate is used for supplementing ammonia nitrogen concentration to 250mg/L, sodium chloride is used for supplementing to ensure that the total salt content of the culture medium reaches 10000mg/L, and the pH value is kept at 6.5-8.5; then replacing the culture medium every 2-4 days, and in addition, supplementing ammonium sulfate to increase the ammonia nitrogen concentration by 100mg/L on the basis of the previous culture every 4-7 days, and supplementing sodium chloride to increase the total salt concentration by 10000mg/L on the basis of the previous culture until the ammonia nitrogen concentration of the culture medium is increased to 550mg/L and the total salt is increased to 40000 mg/L; the acclimation time is 20-35 days;
the culture solution concentration method in the step (3) is centrifugation for 1-20min at the rotating speed of 1000-;
when the protective solution is sodium nitrite solution, the concentration of the protective solution is 5mg/L-50mg/L, and the mass ratio of the concentrated thallus to the protective solution is 1:1-1: 8;
when the protective solution is trehalose solution or inorganic salt culture medium solution special for nitrobacteria, the concentration of the protective solution is 200mg/L-1000 mg/L; and the mass ratio of the concentrated thallus to the protective solution is 1:3-1: 9;
the concentrated bacteriaCulturing in protective solution for 10min-2 h; the weight percentage of water in the mixed solution of the concentrated thallus and the protective solution is detected simultaneously and recorded as W 1 。
2. The method for preparing a semi-dry nitrobacter agent according to claim 1, characterized in that: in the step (1), the aerobic activated sludge is required to be kept stand for 1-3h, supernatant is discarded and then used as a bacteria source, and the weight ratio of the bacteria source to the inorganic salt culture medium special for nitrobacteria is 1:9-4: 6.
3. The method for preparing a semi-dry nitrobacter agent according to claim 1, characterized in that: the enrichment culture method in the step (1) comprises the following steps: aerating the culture medium by using an electromagnetic oxygenation pump, and maintaining the dissolved oxygen at 2.0-6.0 mg/L; the temperature is 20-35 deg.C, the culture medium is changed every day, ammonium sulfate is used to supplement the ammonia nitrogen of the culture medium to reach concentration of 50-150mg/L, pH is kept at 6.5-8.5, and the enrichment culture time is 5-7 days.
4. The method for preparing a semi-dry nitrobacter agent according to claim 1, characterized in that: the carrier in the step (4) is one or more of diatomite, kaolin, zeolite powder or bentonite; the treatment mode of the carrier is a damp-heat sterilization method, and the carrier is sterilized for 15-30min at 115 ℃; the mass ratio of the mixed solution of the concentrated thalli and the protective solution to the carrier is recorded as X: Y, and the condition W is met 1 *X=（10%-50%）*（X+Y）。
5. The method for preparing a semi-dry nitrobacter agent according to claim 1, characterized in that: the nitration rate of the finally obtained nitrating bacteria agent is more than 500mg/(L.d), the nitrating bacteria agent is in a semi-dry solid state, and the water content is 10-50%.
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| CN110551657A (en) * | 2019-09-11 | 2019-12-10 | 广东中微环保生物科技有限公司 | Preparation method of solid composite nitrifying bacteria agent |
| CN110564619A (en) * | 2019-10-15 | 2019-12-13 | 山东海景天环保科技股份公司 | nitrifying bacteria agent and preparation method thereof |
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