CN109477111B - 热稳定聚合酶抑制剂组合物和方法 - Google Patents

热稳定聚合酶抑制剂组合物和方法 Download PDF

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CN109477111B
CN109477111B CN201780045522.5A CN201780045522A CN109477111B CN 109477111 B CN109477111 B CN 109477111B CN 201780045522 A CN201780045522 A CN 201780045522A CN 109477111 B CN109477111 B CN 109477111B
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aptamer
composition
sequence
seq
polymerase
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CN109477111A (zh
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S·G·洛霍夫
A·A·高尔
V·巴拉兹奈诺克
E·V·维亚佐夫基那
I·希什金娜
D·H·佩尔森
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Cepheid
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
    • C—CHEMISTRY; METALLURGY
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/532—Closed or circular
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2320/00—Applications; Uses
    • C12N2320/50—Methods for regulating/modulating their activity
    • C12N2320/52—Methods for regulating/modulating their activity modulating the physical stability, e.g. GC-content
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/205—Aptamer

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Wood Science & Technology (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
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  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201780045522.5A 2016-06-07 2017-06-06 热稳定聚合酶抑制剂组合物和方法 Active CN109477111B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662347004P 2016-06-07 2016-06-07
US62/347,004 2016-06-07
PCT/US2017/036225 WO2017214202A1 (en) 2016-06-07 2017-06-06 Thermostable polymerase inhibitor compositions and methods

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CN109477111A CN109477111A (zh) 2019-03-15
CN109477111B true CN109477111B (zh) 2023-12-15

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US (3) US11299739B2 (enExample)
EP (1) EP3464596B1 (enExample)
JP (2) JP7106461B2 (enExample)
CN (1) CN109477111B (enExample)
AU (1) AU2017277304B2 (enExample)
CA (1) CA3026646A1 (enExample)
WO (1) WO2017214202A1 (enExample)

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EP3464596B1 (en) 2016-06-07 2025-07-30 Cepheid Thermostable polymerase inhibitor compositions and methods
EP3818170A4 (en) * 2018-07-02 2022-03-09 Swift Biosciences, Inc. Temperature controlled dna polymerase inhibitors
KR102950624B1 (ko) 2018-07-24 2026-04-10 플래그쉽 파이어니어링 이노베이션스 브이아이, 엘엘씨 원형 폴리리보뉴클레오티드를 포함하는 조성물 및 이의 용도
US12636369B2 (en) 2019-06-19 2026-05-26 Flagship Pioneering Innovations Vi, Llc Compositions comprising circular polyribonucleotides for protein modulation and uses thereof
CN114990126B (zh) * 2022-08-03 2022-11-18 珠海宝锐生物科技有限公司 特异性结合Bst DNA聚合酶大片段活性位点的核酸适配体及应用
CN116004776A (zh) * 2022-08-30 2023-04-25 上海伯杰医疗科技股份有限公司 一管式RT-qPCR全预混反应试剂、应用及RT-qPCR方法
WO2026136820A1 (en) 2024-12-20 2026-06-25 Cepheid Dna polymerase inhibitor

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CN102686732A (zh) * 2009-08-17 2012-09-19 吉尼松公司 基于杆状病毒生产不含污染性杆状病毒毒粒的生物药品
WO2015091720A1 (en) * 2013-12-20 2015-06-25 Roche Diagnostics Gmbh An improved oligonucleotide inhibitor of dna polymerases
WO2016072758A1 (ko) * 2014-11-04 2016-05-12 한국과학기술원 표적 물질에 의해 조절되는 핵산 중합효소 활성을 이용한 생체물질의 검출 및 정량 방법

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CN102686732A (zh) * 2009-08-17 2012-09-19 吉尼松公司 基于杆状病毒生产不含污染性杆状病毒毒粒的生物药品
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Publication number Publication date
CA3026646A1 (en) 2017-12-14
AU2017277304B2 (en) 2023-07-20
US12385045B2 (en) 2025-08-12
JP7106461B2 (ja) 2022-07-26
WO2017214202A1 (en) 2017-12-14
US20220389427A1 (en) 2022-12-08
JP2022088455A (ja) 2022-06-14
US11299739B2 (en) 2022-04-12
US20190316132A1 (en) 2019-10-17
EP3464596B1 (en) 2025-07-30
JP7420850B2 (ja) 2024-01-23
US20250369000A1 (en) 2025-12-04
CN109477111A (zh) 2019-03-15
AU2017277304A1 (en) 2018-12-20
JP2019520815A (ja) 2019-07-25
EP3464596A1 (en) 2019-04-10

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