CN109465293A - A kind of fungi restorative procedure of petroleum hydrocarbon contaminated soil - Google Patents
A kind of fungi restorative procedure of petroleum hydrocarbon contaminated soil Download PDFInfo
- Publication number
- CN109465293A CN109465293A CN201910051242.6A CN201910051242A CN109465293A CN 109465293 A CN109465293 A CN 109465293A CN 201910051242 A CN201910051242 A CN 201910051242A CN 109465293 A CN109465293 A CN 109465293A
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- Prior art keywords
- petroleum hydrocarbon
- fungi
- contaminated soil
- soil
- hydrocarbon contaminated
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Soil Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Processing Of Solid Wastes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
This method discloses a kind of method that white-rot fungi repairs petroleum hydrocarbon contaminated soil.White-rot fungi is interplanted by building furrow in containing petroleum hydrocarbon contaminated farmland.White-rot fungi mycelium by with the contact of petroleum hydrocarbon in soil, decomposed by ectoenzyme, directly absorb etc. modes decompose petroleum hydrocarbon.The present invention is cultivated the full mycelial white-rot fungi bacterium bag of hair into soil by intercropping mode, realizes the purpose that petroleum hydrocarbon is decomposed in the soil.
Description
Technical field
The present invention relates to technical field of environment pollution control, and in particular to decomposes petroleum hydrocarbon in soil using fungi, passes through
Fungus degrading reaches the reparation of petroleum hydrocarbon contaminated soil, i.e., the fungi restorative procedure of a kind of petroleum hydrocarbon contaminated soil.
Background technique
As the main power fuel and industrial chemicals of modern society, petroleum and products thereof is applied to production and life
Various aspects, including industry, agricultural, military affairs, communications and transportation etc..In the exploitation use process of petroleum, since there are process waters
The limitation of gentle processing technique, largely containing the waste water of oily substance, waste residue, exhaust gas enters in soil, destroys soil ecology.
The chemical composition of crude oil is complicated, including a variety of noxious materials such as alkane, cycloalkane, aromatic hydrocarbon, a portion object
Matter also has teratogenesis, carcinogenic, mutagenic effect, is enriched in animal and plant body by food chain, strong to ecological environment and the mankind
Kang Zaocheng grave danger.
In the various administering methods of contaminated soil, microorganism remediation has expense low, high treating effect, and to environment
Many advantages, such as destructive small and be valued by people, be increasingly becoming research hotspot in recent years, be to a certain extent pollution
The reparation of soil brings technical revolution.It is a kind of efficiently enforceable in order to obtain efficient, thorough biological prosthetic effect
Restorative procedure is necessary.
Summary of the invention
It is an object of the invention to provide the fungi restorative procedures of petroleum hydrocarbon contaminated soil, efficiently true by screening domestication
Bacterium realizes the fast decoupled of soil petroleum hydrocarbon by the bacteria stick for sending out full mycelia in cultivation in farmland.
A kind of a kind of fungi restorative procedure of petroleum hydrocarbon contaminated soil of petroleum hydrocarbon contaminated soil is containing petroleum hydrocarbon contaminated soil
It is covered in earth with corn and plants white-rot fungi.
Preferably, the petroleum hydrocarbon contaminated soil is that petroleum hydrocarbon content is lower than 50000mg/kg contaminated soil.
Preferably, at 5-28 DEG C of environment temperature, in the soil containing petroleum hydrocarbon cultivation send out 10-12 bags of the bacteria stick of full bacterium bag/
m2Soil.
Preferably, under Intercropping, scattering illumination condition growth.Fungi is cultivated under wet condition, humid control exists
90%。
Fungi restorative procedure includes the following steps:
1) strain on the inclined-plane PDA of preservation is cultivated 5 days, is grown to mycelium pellet to being inoculated into 100mlPDB fluid nutrient medium
Certain amount, it is spare.PDA culture medium composition: it weighs 200g potato and is cut into small pieces, boil 20min, eighty per cant filtered through gauze, 20g
Glucose, 200g agar, moisturizing to 1000ml, 121 sterilizing 20min.PDB culture medium composition: weighing 200g potato and be cut into small pieces,
Boil 20min, eighty per cant filtered through gauze, 20g glucose, Mg2SO4, 1.5g, KH2PO43g, adjusting pH to 5 moisturizings to 1000ml,
121 sterilizing 20min.
2) preparation of bacteria stick: corncob and wheat bran are mixed according to the ratio of 9:1, a little urea and lime pack envelope is added
Mouth sterilizing.It is a little that the mycelium pellet of culture is poured into bacterium bag one end, sealing is put into hot-house culture 30 days or so and sends out full bacterium to mycelia
Bag, it is spare.
The bacteria stick for sending out full mycelia is taken out from bag, 25 centimeters of furrow are dug in corn field, bacteria stick is embedded in furrow above
Earthing 5cm, with corn intercrop.
Technical characterstic: the fungi restorative procedure of petroleum hydrocarbon contaminated soil of the invention is in the moist soil containing petroleum hydrocarbon
Cultivate white-rot fungi.The best planting conditions of white-rot fungi: at 5-28 DEG C of environment, full mycelia is sent out in cultivation in wet farmland
10-12 bags/m of bacterium bag2Soil.
The white-rot fungi of cultivation can be lower than in 50000mg/kg contaminated soil in petroleum hydrocarbon content to be grown, and decomposes petroleum
Hydrocarbon.
This method discloses a kind of method that white-rot fungi repairs petroleum hydrocarbon contaminated soil.Containing petroleum hydrocarbon contaminated farmland
In by build furrow interplant white-rot fungi.White-rot fungi mycelium by with the contact of petroleum hydrocarbon in soil, decomposed by ectoenzyme,
The directly modes such as absorption decompose petroleum hydrocarbon.The present invention is cultivated the full mycelial white-rot fungi bacterium bag of hair by intercropping mode
Into soil, the purpose that petroleum hydrocarbon is decomposed in the soil is realized.
Specific embodiment
The cultivation and growth of fungi
Mycelia should be allowed to send out before white-rot fungi cultivation expires bacterium bag, and 10 bags/m2Left and right.It is 15 centimetres wide to build 25 centimetres of depths, 20 centimeter length
Furrow, bacteria stick outer bag is removed, bacteria stick is put into furrow, 3-5 centimetres of soil layer is covered above, keeps wet environment, temperature
It is advisable in 10-25 degree.
(1) specific targets are as follows
Pollution depth are as follows: D=0.3m
Volume weight of soils are as follows: W=1200kg/m3
Environment temperature: 15-25 DEG C
The processing target reached: 3000mg petroleum hydrocarbon/kg soil (agricultural land soil standard).
Wherein bacteria stick the preparation method is as follows:
1) strain on the inclined-plane PDA of preservation is cultivated 5 days, is grown to mycelium pellet to being inoculated into 100mlPDB fluid nutrient medium
Certain amount, it is spare.PDA culture medium composition: it weighs 200g potato and is cut into small pieces, boil 20min, eighty per cant filtered through gauze, 20g
Glucose, 200g agar, moisturizing to 1000ml, 121 sterilizing 20min.PDB culture medium composition: weighing 200g potato and be cut into small pieces,
Boil 20min, eighty per cant filtered through gauze, 20g glucose, Mg2SO4, 1.5g, KH2PO43g, adjusting pH to 5 moisturizings to 1000ml,
121 sterilizing 20min.
2) corncob and wheat bran are mixed according to the ratio of 9:1, a little urea and lime pack sealing sterilizing is added.It will training
It is a little that feeding mycelium pellet pours into bacterium bag one end, sealing, is put into hot-house culture 30 days or so and sends out full bacterium bag to mycelia, spare.
Claims (6)
1. a kind of fungi restorative procedure of petroleum hydrocarbon contaminated soil, it is characterised in that: containing in petroleum hydrocarbon contaminated soil with corn
Set plants white-rot fungi.
2. the fungi restorative procedure of petroleum hydrocarbon contaminated soil according to claim 1, it is characterised in that: described petroleum hydrocarbon contaminated
Soil is that petroleum hydrocarbon content is lower than 50000mg/kg contaminated soil.
3. the fungi restorative procedure of the petroleum hydrocarbon contaminated soil according to requiring 1, it is characterised in that: in environment temperature 5-28
DEG C, the 10-12 bags/m of bacteria stick of full bacterium bag is sent out in cultivation in the soil containing petroleum hydrocarbon2Soil.
4. according to the fungi restorative procedure of petroleum hydrocarbon contaminated soil described in right 1, it is characterised in that: under Intercropping, dissipate
Penetrate illumination condition growth.
5. according to the fungi restorative procedure of petroleum hydrocarbon contaminated soil described in right 4, it is characterised in that: cultivate in humid conditions
Fungi, humid control is 90%.
6. according to the fungi restorative procedure of petroleum hydrocarbon contaminated soil described in right 1, characterized by the following steps:
1) strain on the inclined-plane PDA of preservation is cultivated 5 days, is grown to mycelium pellet to being inoculated into 100mlPDB fluid nutrient medium
Certain amount, it is spare；PDA culture medium composition: it weighs 200g potato and is cut into small pieces, boil 20min, eighty per cant filtered through gauze, 20g
Glucose, 200g agar, moisturizing to 1000ml, 121 sterilizing 20min；PDB culture medium composition: weighing 200g potato and be cut into small pieces,
Boil 20min, eighty per cant filtered through gauze, 20g glucose, Mg2SO4, 1.5g, KH2PO43g, adjusting pH to 5 moisturizings to 1000ml,
121 sterilizing 20min；
2) preparation of bacteria stick: corncob and wheat bran are mixed according to the ratio of 9:1, and a little urea is added and lime pack sealing is gone out
Bacterium；It is a little that the mycelium pellet of culture is poured into bacterium bag one end, sealing is put into hot-house culture 30 days or so and sends out full bacterium bag to mycelia, standby
With；
3) bacteria stick for sending out full mycelia is taken out from bag, 25 centimeters of furrow is dug in corn field, bacteria stick is embedded in furrow and is covered above
Native 5cm, with corn intercrop.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910051242.6A CN109465293A (en) | 2019-01-21 | 2019-01-21 | A kind of fungi restorative procedure of petroleum hydrocarbon contaminated soil |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910051242.6A CN109465293A (en) | 2019-01-21 | 2019-01-21 | A kind of fungi restorative procedure of petroleum hydrocarbon contaminated soil |
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| Publication Number | Publication Date |
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| CN109465293A true CN109465293A (en) | 2019-03-15 |
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ID=65678775
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| Application Number | Title | Priority Date | Filing Date |
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| CN201910051242.6A Pending CN109465293A (en) | 2019-01-21 | 2019-01-21 | A kind of fungi restorative procedure of petroleum hydrocarbon contaminated soil |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022068092A1 (en) * | 2020-09-30 | 2022-04-07 | 中国科学院天津工业生物技术研究所 | Use of mycelium material in oil absorption |
| CN116000082A (en) * | 2021-10-21 | 2023-04-25 | 中国石油化工股份有限公司 | Soil remediation method based on composite microbial preparation |
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| CN1545845A (en) * | 2003-12-02 | 2004-11-17 | 中国科学院东北地理与农业生态研究所 | Intercropping cultivation method for maize and pleurotu ostreatus |
| US7473546B2 (en) * | 2003-11-13 | 2009-01-06 | Savannah River Nuclear Solutions, Llc | Surfactant biocatalyst for remediation of recalcitrant organics and heavy metals |
| CN102652957A (en) * | 2012-05-08 | 2012-09-05 | 江苏大学 | Remediation method of soil polycyclic aromatic hydrocarbon by combining surfactant producing bacteria and mycorrhiza |
| CN108906866A (en) * | 2018-06-27 | 2018-11-30 | 北京高能时代环境技术股份有限公司 | The restorative procedure of petroleum hydrocarbon contaminated soil |
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2019
- 2019-01-21 CN CN201910051242.6A patent/CN109465293A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7473546B2 (en) * | 2003-11-13 | 2009-01-06 | Savannah River Nuclear Solutions, Llc | Surfactant biocatalyst for remediation of recalcitrant organics and heavy metals |
| CN1545845A (en) * | 2003-12-02 | 2004-11-17 | 中国科学院东北地理与农业生态研究所 | Intercropping cultivation method for maize and pleurotu ostreatus |
| CN102652957A (en) * | 2012-05-08 | 2012-09-05 | 江苏大学 | Remediation method of soil polycyclic aromatic hydrocarbon by combining surfactant producing bacteria and mycorrhiza |
| CN108906866A (en) * | 2018-06-27 | 2018-11-30 | 北京高能时代环境技术股份有限公司 | The restorative procedure of petroleum hydrocarbon contaminated soil |
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| 杨辉 等: "多环芳烃污染土壤生物修复技术研究进展", 《安徽农业科学》 * |
| 王倩 等: "Ｔｒａｍｅｔｅｓ ｓｐ．ＭＡ－Ｘ０１漆酶对不同染料的脱色作用", 《安徽科技学院学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022068092A1 (en) * | 2020-09-30 | 2022-04-07 | 中国科学院天津工业生物技术研究所 | Use of mycelium material in oil absorption |
| CN116000082A (en) * | 2021-10-21 | 2023-04-25 | 中国石油化工股份有限公司 | Soil remediation method based on composite microbial preparation |
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