CN109423464A - The actinomycetes strain W15 and its unwrapping wire bacteria agent for preventing and treating ash arrhizus bacteria - Google Patents
The actinomycetes strain W15 and its unwrapping wire bacteria agent for preventing and treating ash arrhizus bacteria Download PDFInfo
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- CN109423464A CN109423464A CN201710783957.1A CN201710783957A CN109423464A CN 109423464 A CN109423464 A CN 109423464A CN 201710783957 A CN201710783957 A CN 201710783957A CN 109423464 A CN109423464 A CN 109423464A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/465—Streptomyces
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention discloses a kind of actinomycetes strains for preventing and treating ash arrhizus bacteria, belong to technical field of plant protection.The bacterial strain is streptomyces (Streptomyces sp.), entitled actinomyces W15.The single colonie of the actinomycetes strain is circle, and brick-red, surface is dry and opaque；Mycelia is thinner, slow growth, and bacterium colony quality is fine and close, and has mud fishy smell.The actinomycetes strain Gram's staining is purple, for the positive.W15 microbial inoculum is 60% or more to the preventive effect of gray mold.The present invention is not easy the actinomycetes strain and its unwrapping wire bacteria agent for so that ash arrhizus bacteria is generated resistance, prevention and treatment ash arrhizus bacteria free from environmental pollution, which can be used after tomato emerges by pouring root.
Description
Technical field
The present invention relates to a kind of microorganism fields, and in particular to it is a kind of prevent and treat ash arrhizus bacteria actinomycetes strain W15 and its
Unwrapping wire bacteria agent.
Background technique
Tomato is one of most important vegetables in agricultural production, and the graw mold of tomato as caused by ash arrhizus bacteria is to endanger tomato
The worldwide disease of production.Gray mold is the fungal disease as caused by Botrytis cinerea, currently, having become a kind of global range
Fungal disease.Botrytis cinerea can endanger blade, flower and fruit, wherein aggrieved mostly important with fruit, grave illness field fruit corruption rate can
Up to 40%.Botrytis cinerea can infect 235 kinds of vegetables, fruit tree and other industrial crops, once morbidity will cause largely to subtract
It produces, or even total crop failure, causes significant damage to agricultural production.
Gray mold is that open country, protecting field crop be common and the disease of more difficult prevention and treatment, and 20-25 DEG C of temperature, humidity are lasting
It is the disease high-incidence season when 90% or more.The sclerotial germination aerial mycelium and conidiophore of ash arrhizus bacteria and conidium, point
It falls off after raw spore is mature, is propagated by means of air-flow, rainwater or dewdrop and farming operations.In 18~23 DEG C and constant relative humidity
Under 90% or more how wet, low light condition, crops susceptible disease；Then morbidity obviously subtracts when temperature is lower than 15 DEG C or higher than 25 DEG C
Gently；Temperature is not fallen ill substantially when being higher than 30 DEG C；Conidium can repeatedly infect again.
Tamato fruit is caught an illness, and Chinese olive is aggrieved heavy, and remaining column cap or petal are mostly first infected, and backward fruit extension causes
Pericarp is in canescence, and has the mould layer of thick grey, is in water corruption shape, blade is fallen ill since blade tip, at " V " shape between vein
Inwardly extended, taupe, while the line shape line for having the depth alternate, sick key has a common boundary clearly demarcated.All the time, chemical prevention is all control ash
The major measure of mildew.Due to the features such as ash arrhizus bacteria growth cycle is short, and sporulation quantity is big, the germ is caused to be easy to generate anti-medicine
Property, furthermore long-term a large amount of uses of chemical agent bring serious threat to environment and food safety, therefore are badly in need of seeking new
Control measure.Develop a kind of pair of environment, the non-chemical control method of safety of human and livestock have become prevention and treatment graw mold of tomato emphasis,
And using in soil actinomyces and unwrapping wire bacteria agent come to carry out biological control be one of the most effective ways.
A kind of it is not easy to make that botrytis cinerea generates resistance, prevention and treatment ash arrhizus bacteria free from environmental pollution is put currently, lacking
Line bacteria strain and its unwrapping wire bacteria agent.
Summary of the invention
Technical problem to be solved by the invention is to provide one kind to be not easy to make ash arrhizus bacteria to generate resistance, free from environmental pollution
Prevention and treatment ash arrhizus bacteria actinomycetes strain W15 and its unwrapping wire bacteria agent.
To achieve the goals above, the present invention is achieved through the following technical solutions: a kind of actinomyces preventing and treating ash arrhizus bacteria
Bacterial strain, the bacterial strain are streptomycete (Streptomyces sp.)；Entitled actinomyces W15.
Further, the circle of the actinomycetes strain, brick-red, surface is dry and opaque；Mycelia is thinner, and growth is slow
Slowly, bacterium colony quality is fine and close, and has mud fishy smell.
Further, the actinomycetes strain Gram's staining is purple, for the positive.
The unwrapping wire bacteria agent of the prevention and treatment ash arrhizus bacteria of actinomyces preparation of the present invention.
Further, the unwrapping wire bacteria agent of the prevention and treatment ash arrhizus bacteria, active constituent are as follows (a), (b) and (c)
At least one of:
(a) actinomyces described in claim 1；
(b) fermented supernatant fluid of actinomyces described in claim 1；
(c) fermentation culture medium of actinomyces described in claim 1.
The preparation method of the unwrapping wire bacteria agent of prevention and treatment ash arrhizus bacteria of the present invention, includes the following steps:
It (1) is streptomyces by the actinomycetes strain W15, Actinomycetes species are upper in Gause I culture medium to be grown,
Temperature is 27-29 DEG C, and dark place is cultivated in constant incubator, and incubation time 2-4d obtains actinomycetes strain；
(2) according to described in above-mentioned steps (1), as Actinomycetes species culture 2-4d, Actinomycetes species are inoculated in
In 50-60ml fermentation medium, temperature is 27-29 DEG C, revolving speed is shaken cultivation 72-74h under 160-180r/min, is then being turned
Speed is to be centrifuged 19-21min under 7000-8000r/min, abandons bottom precipitation, takes fermentation supernatant thin by 0.2 μm
The filtering of bacterium filter, is made unwrapping wire bacteria agent.
Further, in step (1), the Gause I culture medium is by soluble starch 19.9-20.1g, sodium chloride
0.49-0.51g, potassium nitrate 0.9-1.1g, dipotassium hydrogen phosphate 0.49-0.51g, magnesium sulfate 0.49-0.51g, ferrous sulfate
Said components are settled to 1000ml with distilled water, in temperature by 0.009-0.011g, agar 15-20g composition, pH 7.2-7.4
It is the 19-21min that sterilizes at 120-121 DEG C；In step (2), the actinomycete fermentation culture medium by corn flour 9.9-10.1g,
Sucrose 9.9-10.1g, sodium chloride 1.9-2.1g, ammonium sulfate 2.9-3.1g, calcium carbonate 1.9-2.1g composition, pH 7.2-7.4 are used
Said components are settled to 1000ml by distilled water, and sterilize 19-21min at being 120-121 DEG C in temperature.
Application of the unwrapping wire bacteria agent of the present invention in prevention and treatment ash arrhizus bacteria.
The utility model has the advantages that the present invention provides a kind of being not easy that ash arrhizus bacteria is made to generate resistance, prevention and treatment grey mold free from environmental pollution
The actinomycetes strain W15 and its unwrapping wire bacteria agent of germ.Unwrapping wire bacteria agent belongs to biological agent, with chemistry caused by chemical agent
The problems such as drug resistance, is different, is a kind of biological approach of the prevention and treatment ash arrhizus bacteria of safety non-pollution, thus is conducive to the nothing of tomato
Public hazards production, meets the requirement of agricultural sustainable development.Peasant can not have to during tomato production or reduce other prevention and treatments
The dosage of the pesticide of botrytis cinerea, and then reduce peasant and reduce expenses.Meanwhile the unwrapping wire bacteria agent prevents ash arrhizus bacteria
Effect is controlled up to 60% or more.
Specific embodiment
It will be specifically described, but be not to be construed as to the present invention by the way that specific embodiment is further to the present invention below
The restriction of protection scope.
Embodiment 1
The identification of the 16S rRNA molecule of bacterial strain
Actinomycetes strain W15 carries out activation culture, Gause I culture at being 28 DEG C in temperature on Gause I plate
Base: soluble starch 19.9-20.1g, sodium chloride 0.49-0.51g, potassium nitrate 0.9-1.1g, dipotassium hydrogen phosphate 0.49-0.51g,
Magnesium sulfate 0.49-0.51g, ferrous sulfate 0.009-0.011g, agar 15-20g composition, pH 7.2-7.4 will be upper with distilled water
It states component and is settled to 1000ml.
It has been observed that the single colonie of W15 is round after incubation time is 2-4d, brick-red, surface is dry and opaque；Mycelia compared with
Carefully, slow growth, bacterium colony quality is fine and close, and has mud fishy smell.Bacterial strain W15 Gram's staining is purple, for the positive.
The morphological feature and common bacteria system identification handbook Streptomyces category actinomyces (Streptomyces of bacterial strain W15
Sp.) the most similar.
Using W15 genome DNA as template, PCR amplification 16S rRNA obtains the amplified production of length about 1.5Kb, this with
The report that 16S rRNA most highly conserved and consistent length in all bacteriums is 1.5Kb or so coincide.By sequencing result
Blast is searched in the GenBank database of the website NCBI, carries out nucleotide homology ratio with listed 16S rRNA sequence
Compared with, it is found that the 16S rRNA sequence of bacterial strain W15 and streptomyces (Streptomyces sp.) similarity are higher, be greater than 99%,
In conjunction with the bacterial strain cultural character as a result, actinomycetes strain W15 is initially identified as streptomyces actinomyces (Streptomyces
sp.)。
The present invention provides a kind of unwrapping wire for being not easy that ash arrhizus bacteria is made to generate resistance, prevention and treatment ash arrhizus bacteria free from environmental pollution
Bacteria strain W15 and its unwrapping wire bacteria agent.Unwrapping wire bacteria agent belongs to biological agent, asks with chemistry drug resistance etc. caused by chemical agent
Topic is different, is a kind of biological approach of the prevention and treatment ash arrhizus bacteria of safety non-pollution, thus is conducive to the No-harmful apple orchard of tomato, accords with
Close the requirement of agricultural sustainable development.Peasant can not have to during tomato production or reduce other prevention and treatment botrytis cinereas
Pesticide dosage, and then reduce peasant reduce expenses.Meanwhile the unwrapping wire bacteria agent is reachable to the control efficiency of ash arrhizus bacteria
60% or more.
Embodiment 2
A kind of actinomycetes strain of prevention and treatment ash arrhizus bacteria of the invention, the bacterial strain are streptomyces (Streptomyces
Sp.), entitled actinomyces W15.
The single colonie of the actinomycetes strain is circle, and brick-red, surface is dry and opaque；Mycelia is thinner, slow growth,
Bacterium colony quality is fine and close, and has mud fishy smell.The actinomycetes strain Gram's staining is purple, for the positive.
The unwrapping wire bacteria agent of the prevention and treatment ash arrhizus bacteria of actinomyces preparation of the present invention.Its active constituent be following (a),
(b) and at least one of (c):
(a) actinomyces described in claim 1；
(b) fermented supernatant fluid of actinomyces described in claim 1；
(c) fermentation culture medium of actinomyces described in claim 1.
The preparation method of the unwrapping wire bacteria agent of prevention and treatment ash arrhizus bacteria of the present invention, includes the following steps:
It (1) is streptomyces by the actinomycetes strain W15, Actinomycetes species are upper in Gause I culture medium to be grown,
Temperature is 27-29 DEG C, and dark place is cultivated in constant incubator, and incubation time 2-4d obtains actinomycetes strain；Institute
Gause I culture medium is stated by soluble starch 19.9-20.1g, sodium chloride 0.49-0.51g, potassium nitrate 0.9-1.1g, phosphoric acid hydrogen
Dipotassium 0.49-0.51g, magnesium sulfate 0.49-0.51g, ferrous sulfate 0.009-0.011g, agar 15-20g composition, pH 7.2-
7.4, said components are settled to 1000ml with distilled water, sterilize 19-21min at being 120-121 DEG C in temperature；
(2) according to described in above-mentioned steps (1), as Actinomycetes species culture 2-4d, Actinomycetes species are inoculated in
In 50-60ml fermentation medium, temperature is 27-29 DEG C, revolving speed is shaken cultivation 72-74h under 160-180r/min, is then being turned
Speed is to be centrifuged 19-21min under 7000-8000r/min, abandons bottom precipitation, takes fermentation supernatant thin by 0.2 μm
The filtering of bacterium filter, is made unwrapping wire bacteria agent.The actinomycete fermentation culture medium is by corn flour 9.9-10.1g, sucrose 9.9-
10.1g, sodium chloride 1.9-2.1g, ammonium sulfate 2.9-3.1g, calcium carbonate 1.9-2.1g composition, pH 7.2-7.4 will with distilled water
Said components are settled to 1000ml, and sterilize 19-21min at being 120-121 DEG C in temperature.
Application of the unwrapping wire bacteria agent of the present invention in prevention and treatment ash arrhizus bacteria.
The measurement of unwrapping wire bacteria agent plate control efficiency
Gray mold bacterial strain cultivated in PDA culture medium 5 days it is spare；It will cultivate according to the ratio of 1: 10 (v/v) to pre- timing
Between unwrapping wire bacteria agent uniformly mixed with PDA culture medium, be prepared into the culture medium of unwrapping wire bacteria agent of the W15 containing actinomyces (to add
Enter the PDA culture medium of the fermentation medium supernatant of equal proportion as control).It is beaten with punch (d=5mm) and takes gray mold mycelia
Ash arrhizus bacteria bacterium dish is inoculated in above-mentioned culture medium by outer edge, and the 3-8 days after inoculation are every measurement botrytis cinerea for 24 hours
Diameter, plate antagonistic effect of the statistics unwrapping wire bacteria agent to ash arrhizus bacteria.The calculation formula of control efficiency effect is: preventive effect (%)
=(control group botrytis cinerea diameter-processing group botrytis cinerea diameter)/control group botrytis cinerea diameter × 100%.
Situation of change of the unwrapping wire bacteria agent that table 1 is actinomyces W15 to the plate inhibiting rate of botrytis cinerea with number of days.
Table 1
| It is inoculated with number of days | 3 | 4 | 5 | 6 | 7 | 8 |
| 40.15±5.02 | 37.28±3.12 | 35.24±3.25 | 32.15±3.92 | 33.02±2.16 | 31.46±1.84 |
Such as table 1, it can be concluded that, plate control efficiency measurement result shows the W15 unwrapping wire bacteria agent for cultivating 72h to gray mold
The growth of bacterium has a degree of inhibiting effect.When being inoculated with 3d, W15 unwrapping wire bacteria agent is to gray mold percentage mycelial inhibition highest, with
Inoculation number of days increase, have a declining tendency to the inhibiting rate of gray mold mycelia, but inhibiting rate still remain in 30% with
On.Plate antagonistic experiment shows the unwrapping wire bacteria agent active material that the actinomyces generate when cultivating 72h of actinomyces W15 to ash
The growth of mildew mycelia has a degree of inhibiting effect.Average value ± the standard error of numerical value inhibiting rate in table.
Embodiment 3
The measurement of excised leaf control efficiency
The culture of unwrapping wire bacteria agent is spare to the scheduled time.It is acquired on the tomato plant neat from growth of the same size
Healthy leaves are gently rinsed after impregnating tomato leaf agreement that contracts a film or TV play to an actor or actress 10s with the polysorbas20 that concentration is 0.5% with sterile water, are taken out ultra-clean
It is dried in workbench.1min is impregnated after blade drying in unwrapping wire bacteria agent, taking-up is dried in superclean bench (with actinomyces
Fermentation medium supernatant immersion treatment blade is as control), the botrytis cinerea bacterium that diameter is 5mm is inoculated on blade
Dish, each processing are repeated 8 times.Lesion diameter is measured after 25 DEG C, relative humidity 80-90%, dark condition culture 48h, assessment is anti-
Effect.The calculation formula of preventive effect is: preventive effect (%)=(control group lesion diameter-processing group lesion diameter)/control group lesion diameter
× 100%.
The unwrapping wire bacteria agent that table 2 is actinomyces W15 measures the excised leaf control efficiency of graw mold of tomato.
Table 2
Such as table 2, it can be concluded that, excised leaf control efficiency is the experimental results showed that cultivate the W15 unwrapping wire bacteria agent of 72h to ash
Mildew bacterium has a degree of control efficiency in vitro tomato leaf.Different letters is indicated in 0.05 horizontal upper difference in table
Significantly.
Embodiment 4
The measurement of potting control efficiency
Embodiment 4 and the difference of embodiment 3 are: unwrapping wire bacteria agent is poured into about 1 month tomato seedling root of growth,
Every plant of tomato fills 3ml (pouring into the seedling of isometric fermentation medium supernatant with root position as control).After pouring root 3d,
Inoculating tomato ash arrhizus bacteria mycelia block in basin alms bowl, 20 plants of tomato plants of each processing inoculation.It is cultivated at 25 DEG C, calculates plant after being inoculated with 4d
Strain disease incidence and control efficiency.Each parameter calculation formula is as follows:
Disease incidence (%)=(disease plant number/total plant number) × 100%
Control efficiency (%)=[(control group disease incidence-processing group disease incidence)/control group disease incidence] × 100%
Table 3 is that actinomyces W15 unwrapping wire bacteria agent measures the potting control efficiency of graw mold of tomato.
Table 3
It can be concluded that, detected after inoculation gray mold mycelia block 4 days, potted plant experiment is the result shows that by actinomyces such as table 3
The unwrapping wire bacteria agent root irrigation of W15, tomato plant can effectively resist infecting for graw mold of tomato, and preventive effect is up to 62.62%；In table
Different letters is indicated in 0.05 horizontal upper significant difference.
It can prove that using unwrapping wire bacteria agent of the present invention as the medicament of effective component be one according to the above various embodiments
The novel agricultural bacteriocide of kind.This medicament, which is especially, shows brilliant effect to ash arrhizus bacteria, and environmental pollution is small,
The low feature of drug resistance.
The basic principles, main features and advantages of the present invention have been shown and described above.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, the present invention
Claimed range is delineated by the appended claims, the specification and equivalents thereof from the appended claims.
Claims (8)
1. a kind of actinomycetes strain for preventing and treating ash arrhizus bacteria, it is characterised in that: the bacterial strain is that the bacterial strain is streptomyces
(Streptomyces sp.), entitled actinomyces W15.
2. the actinomycetes strain of prevention and treatment ash arrhizus bacteria according to claim 1, it is characterised in that: the actinomycetes strain
Single colonie is circle, and brick-red, surface is dry and opaque；Mycelia is thinner, slow growth, and bacterium colony quality is fine and close, and has mud raw meat
Taste.
3. the actinomycetes strain of prevention and treatment ash arrhizus bacteria according to claim 2, it is characterised in that: the actinomycetes strain leather
Blue Albert'stain Albert is purple, for the positive.
4. the unwrapping wire bacteria agent of the prevention and treatment ash arrhizus bacteria of actinomyces preparation according to claim 1.
5. the unwrapping wire bacteria agent of prevention and treatment ash arrhizus bacteria according to claim 4, active constituent is following (a), (b) and
At least one of (c):
(a) actinomyces described in claim 1；
(b) fermented supernatant fluid of actinomyces described in claim 1；
(c) fermentation culture medium of actinomyces described in claim 1.
6. the method for preparing the unwrapping wire bacteria agent of prevention and treatment ash arrhizus bacteria as claimed in claim 1 or 2, it is characterised in that including walking as follows
It is rapid:
It (1) is streptomyces, Actinomycetes species upper growth, temperature in Gause I culture medium by the actinomycetes strain W15
It is 27-29 DEG C, dark place is cultivated in constant incubator, and incubation time 2-4d obtains actinomycetes strain；
(2) according to described in above-mentioned steps (1), as Actinomycetes species culture 2-4d, Actinomycetes species are inoculated in 50-
In 60ml fermentation medium, temperature is 27-29 DEG C, revolving speed is shaken cultivation 72-74h under 160-180r/min, then in revolving speed
For 19-21min is centrifuged under 7000-8000r/min, bottom precipitation is abandoned, fermentation supernatant is taken to pass through 0.2 μm of bacterium
Filter filtering, is made unwrapping wire bacteria agent.
7. the preparation method of unwrapping wire bacteria agent according to claim 1, it is characterised in that: in step (1), the Gao Shi
No.1 culture medium is by soluble starch 19.9-20.1g, sodium chloride 0.49-0.51g, potassium nitrate 0.9-1.1g, dipotassium hydrogen phosphate
0.49-0.51g, magnesium sulfate 0.49-0.51g, ferrous sulfate 0.009-0.011g, agar 15-20g composition, pH 7.2-7.4,
Said components are settled to 1000ml with distilled water, sterilize 19-21min at being 120-121 DEG C in temperature；In step (2), institute
Actinomycete fermentation culture medium is stated by corn flour 9.9-10.1g, sucrose 9.9-10.1g, sodium chloride 1.9-2.1g, ammonium sulfate 2.9-
Said components are settled to 1000ml with distilled water, are in temperature by 3.1g, calcium carbonate 1.9-2.1g composition, pH 7.2-7.4
Sterilize 19-21min at 120-121 DEG C.
8. the claim 4~5 unwrapping wire bacteria agent is in the application of prevention and treatment ash arrhizus bacteria.
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| CN101698827A (en) * | 2009-09-10 | 2010-04-28 | 中国农业大学 | Erythrochromogenes and use thereof in biological control of diseases |
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| CN104872194A (en) * | 2015-04-13 | 2015-09-02 | 天津科技大学 | Application of streptomyces alboflavus in prevention and treatment of botrytis cinerea |
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2017
- 2017-08-31 CN CN201710783957.1A patent/CN109423464A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101698827A (en) * | 2009-09-10 | 2010-04-28 | 中国农业大学 | Erythrochromogenes and use thereof in biological control of diseases |
| CN103222483A (en) * | 2013-01-07 | 2013-07-31 | 中国计量学院 | Use of Streptomyces sioyaensis metabolite in prevention and control of Botrytis cinerea |
| CN104872194A (en) * | 2015-04-13 | 2015-09-02 | 天津科技大学 | Application of streptomyces alboflavus in prevention and treatment of botrytis cinerea |
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| Title |
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| 徐大勇等: "番茄灰霉病拮抗内生放线菌的筛选、鉴定及其活性评价", 《生态学杂志》 * |
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