CN109422818A - A kind of preparation method and applications of Polysaccharides from Leaves of Moringa oleifera MLP20-1 - Google Patents
A kind of preparation method and applications of Polysaccharides from Leaves of Moringa oleifera MLP20-1 Download PDFInfo
- Publication number
- CN109422818A CN109422818A CN201710738449.1A CN201710738449A CN109422818A CN 109422818 A CN109422818 A CN 109422818A CN 201710738449 A CN201710738449 A CN 201710738449A CN 109422818 A CN109422818 A CN 109422818A
- Authority
- CN
- China
- Prior art keywords
- polysaccharides
- leaves
- moringa oleifera
- moringa
- alpha
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The preparation method and applications of the present invention relates to a kind of Polysaccharides from Leaves of Moringa oleifera MLP20-1 with alpha-glucoside inhibiting activity.It can be applied to prepare the purposes of the drug of hypoglycemic drug and treatment obesity the present invention provides the preparation method of Polysaccharides from Leaves of Moringa oleifera MLP20-1, the alpha-glucoside inhibiting activity of the polysaccharide and the invention.
Description
Technical field
It the preparation method of the present invention relates to a kind of Polysaccharides from Leaves of Moringa oleifera MLP20-1 with alpha-glucoside inhibiting activity and its answers
With belonging to field of natural product chemistry, polysaccharide obtained has alpha-glucoside inhibiting activity, has weight in the treatment of diabetes
The application value wanted.
Background technique
Moringa is a kind of perennial deciduous tree, and plantation is in the torrid zone and subtropical countries extensively.The stem of Moringa, leaf, root,
It spends equal positions all edible, is commonly used for woody vegetables in country in Southeast Asia.Moringa seed fat content is abundant, also containing a large amount of
Amino acid, potassium, zinc, amino acid, vitamin and high unsaturated fatty acid, protein, sterol etc., by as high nutrition vegetables
It is developed and used with medicinal material.Moringa heat resistance, drought tolerance are stronger, and growth rapidly, because of its nutritive value rich in, is widely answered
People are obtained with prospect more and more to favor, and nowadays, have obtained the kind of large area in the Yunnan in China, Guangdong, Fujian Moringa
It plants.In addition to nutritive value rich in, Moringa also has stronger anti-oxidant, antibacterial, anti-inflammatory, antiviral, antiulcer activity,
Moringa can be used for clinically relieving pain that decompression, epidemic prevention power is low, treatment hypertension and diabetes etc., be particularly applied to
Baby and ursing mother aspect, fight trophic disturbance and prevention disease shows more significant effect.
Diabetes, pathology are complicated, and main feature shows as that hyperglycemia, Glucose tolerance test are low, and illness initial stage is without obvious
Symptom, later period just will appear the symptom of " three-many-one-little ".Currently, the main reason for basal plasma glucose amount height is initiation complication, because
This inhibits sugar decomposition in the clinical treatment of diabetes, and alleviating sugar to absorb is one of important measure.Alpha-glucosidase is one group of presence
In disaccharide hydrolase, including maltose, invertase, lactase etc. in intestinal villi film brush border.It will be double in food
Sugar and oligosaccharide hydrolysis are glucose, blood circulation are absorbed by intestinal epithelial cell, so as to cause postprandial blood sugar water
Flat raising.Therefore, the activity of level of postprandial blood sugar and alpha-glucosidase has close relationship, and alpha-glucosidase inhibitor is as one
The hypoglycemic drug of kind is got growing concern for, and has several alpha-glucosidase inhibitors exploitation listings, such as Ah Ka at present
Wave sugar, Miglitol etc..
Polysaccharides from Leaves of Moringa oleifera prepared by the present invention has stronger alpha-glucoside inhibiting activity, can be applied to prepare hypoglycemic agent
Object and the drug for treating obesity.
Summary of the invention
The preparation of the purpose of the present invention is to provide a kind of Polysaccharides from Leaves of Moringa oleifera MLP20-1 with alpha-glucoside inhibiting activity
Method and its application.
The present invention is achieved through the following technical solutions:
A kind of preparation method of the Polysaccharides from Leaves of Moringa oleifera with alpha-glucoside inhibiting activity, comprising the following steps:
(1) leaf of Moringa that 100g crushes drying is weighed, extracts 120min at 100 DEG C according to 1: 10-1: 20 solid-liquid ratio,
65 DEG C of ultrasonic water baths handle 1h, and obtained leaf of Moringa Thick many candies extracting solution is placed in 56 DEG C, and inspissation contracting is hanged under 800W, is added 3 times
The dehydrated alcohol precipitates overnight of volume is centrifuged, and is redissolved.Using complex enzyme formulation, (alkali protease: trypsase=8: 2) method is de-
Albumen repeats 2-3 times, up to no white flock precipitate is precipitated.
(2) 20% alcohol precipitations
Dehydrated alcohol is added into the Polysaccharides from Leaves of Moringa oleifera solution after de- albumen, makes the volume 20% of dehydrated alcohol, at 4 DEG C
Alcohol precipitation is stayed overnight, and is centrifuged, is removed supernatant, obtains 20% alcohol precipitation Polysaccharides from Leaves of Moringa oleifera extracting solution.
(3) anion exchange chromatography isolates and purifies
Polysaccharides from Leaves of Moringa oleifera extracting solution obtained in (2) step is redissolved, solution passes through DEAE-Sepharose Fast
The separation of Flow ion-exchange chromatography is eluted with the NaCl solution of 0-1.0M, and eluent is concentrated through dialysis, vacuum freeze drying,
Obtain Polysaccharides from Leaves of Moringa oleifera MPL20-1 powder.
A kind of application of the Moringa stem polysaccharide MPL20-1 with alpha-glucoside inhibiting activity, which is characterized in that such Moringa
The recovery rate of leaf polyose is high, has preferable inhibitory activity to alpha-glucosidase, and can effectively adjust the diabetic mice of STZ induction
Blood glucose level, can be applied to hypoglycemic drug and treat diabetes, obesity drug preparation.
Detailed description of the invention
Fig. 1: the DEAE-Sepharose Fast Flow elution curve of 20% alcohol precipitation component of Polysaccharides from Leaves of Moringa oleifera
Fig. 2: the external inhibitory activity of the alpha-glucosidase of Polysaccharides from Leaves of Moringa oleifera MLP20-1
Specific embodiment
In the following, the present invention is described further in conjunction with the embodiments, but the present invention is limited in any way,
For those skilled in the art, the invention may be variously modified and varied.It is all the spirit and principles in the present invention it
Any modification, equivalent substitution, improvement and etc. that are interior, being done, should be included among protection of the invention.
The preparation of 1 Polysaccharides from Leaves of Moringa oleifera MLP20-1 of embodiment
(1) leaf of Moringa that 100g crushes drying is weighed, extracts 120min at 100 DEG C according to 1: 20 solid-liquid ratio, 65 DEG C
Ultrasonic water bath handles 1h, and obtained leaf of Moringa Thick many candies extracting solution is placed in 56 DEG C, and inspissation contracting is hanged under 800W, 3 times of volumes are added
Dehydrated alcohol precipitates overnight, be centrifuged, redissolve.Using complex enzyme formulation, (alkali protease: trypsase=8: 2) method takes off egg
It is white, it is repeated 3 times, up to no white flock precipitate is precipitated.
(2) 20% alcohol precipitations
Dehydrated alcohol is added into the Polysaccharides from Leaves of Moringa oleifera solution after de- albumen, makes the volume 20% of dehydrated alcohol, at 4 DEG C
Alcohol precipitation is stayed overnight, and is centrifuged, is removed supernatant, obtains the Polysaccharides from Leaves of Moringa oleifera extracting solution of 20% alcohol precipitation gradient.
(3) anion exchange chromatography isolates and purifies
Polysaccharides from Leaves of Moringa oleifera extracting solution obtained in (2) step is redissolved, solution passes through DEAE-Sepharose Fast
The separation of Flow ion-exchange chromatography is eluted with the NaCl solution of 0-1.0M, and eluant component MLP20-1 is concentrated through dialysis, very
Vacuum freecing-dry obtains 20% alcohol precipitation gradient Polysaccharides from Leaves of Moringa oleifera powder MLP20-1.
The external inhibitory activity of alpha-glucosidase of 2 Polysaccharides from Leaves of Moringa oleifera MLP20-1 of embodiment
(1) preparation of solution: PBS solution: 0.1M, PH=6.9；PNPG:5mM, PBS solution dissolve p-nitrophenyl-α-
D- glucopyranoside solution.Alpha-glucosidase solution: 1.0U/mL, PBS solution dissolve alpha-glucosidase.Acarbose standard
Solution: 0.1-0.5mg/mL.
(2) measurement of alpha-glucoside inhibiting activity: living with P- nitrophenol method measurement alpha-glucosidase using PNPG as substrate
Property, the generating rate of nitrophenol and the activity of alpha-glucosidase are directly proportional.Concrete operation step are as follows: to 50 μ L embodiments 1 and in fact
It applies and 100 μ L alpha-glucosidase solution is added in each component polysaccharide solution (0.1-0.5mg/mL) that example 2 obtains, reacted at 25 DEG C
Absorbance value is measured at 10min, 405nm, is then proceeded to the PNPG that 50 μ L are added, is reacted 5min at 25 DEG C, survey again at 405nm
Determine absorbance value.With 0.1M in blank group, the PBS of PH=6.9 replaces polysaccharide solution, in standard group with 0.1-0.5mg/mL Ah
Card wave Standard for Sugars solution replaces polysaccharide solution.The inhibiting rate of alpha-glucosidase is calculated as follows:
%inhibition=[(Δ AbsBlank group-ΔAbsExperimental group)/ΔAbsBlank group]×100
Calculate Polysaccharides from Leaves of Moringa oleifera MLP20-1 to the inhibitory activity of alpha-glucosidase, as a result as shown in Figure 1.The IC of MLP20-150
For 0.082mg/mL.
Influence of the 3 Polysaccharides from Leaves of Moringa oleifera MLP20-1 of embodiment to the STZ diabetic mice sugar tolerance induced
Kunming is male, Female Rats each 15, weight 20-25kg, be divided into empty stomach group, control group, Bai Tangping group, small dose group,
Middle dose group, large dosage of group.After fasting 20h tail vein registration 50mg/kg STZ, choose blood glucose level be greater than 15mol/L and
The mouse that blood glucose level is not much different continues the experiment of next step.Group stomach-filling physiological saline on an empty stomach, control group stomach-filling physiological saline
With starch 5g/kg, Bai Tangping group stomach-filling starch and Bai Tangping 50mg/kg, small dose group, middle dose group, large dosage group fill respectively
The Polysaccharides from Leaves of Moringa oleifera of stomach 50mg/kg, 100mg/kg, 200mg/kg, measurement polysaccharide is horizontal after 1h is administered.Determination of blood glucose level result
As shown in table 1.
Influence of the 1. Polysaccharides from Leaves of Moringa oleifera MLP20-1 of table to the STZ diabetic mice sugar tolerance induced
The Fourier spectrum of 4 Polysaccharides from Leaves of Moringa oleifera MLP20-1 of embodiment is analyzed
FTIR spectrum analysis (German BRUKER, model VERTEX7.0) is carried out to Polysaccharides from Leaves of Moringa oleifera MLP20-1,
The result shows that the polysaccharide has O-H stretching vibration peak at 3411.32cm-1；2937.51cm-1 place has-CH stretching vibration
Peak；2152.43cm-1 place has cumulated double bond stretching vibration peak；1610.70cm-1 place has C=O stretching vibration peak；
1414.33cm-1 place has C-H stretching vibration peak；1242.13-1039.65cm-1 place has pyranose ring structure stretching vibration peak；
Occurring stretching vibration peak explanation at 915.44cm-1, there are β-glucosides bond structures；777.56cm-1 there is stretching vibration peak explanation in place
There are α-glucosides bond structures；Caused by absorption peak at 536.47cm-1 is CCO deformation vibration.
Claims (2)
1. a kind of preparation method of the Polysaccharides from Leaves of Moringa oleifera MLP2-10 with alpha-glucoside inhibiting activity, includes the following steps
(1) leaf of Moringa that 100g crushes drying is weighed, extracts 120min at 100 DEG C according to 1: 10-1: 20 solid-liquid ratio, 65 DEG C
Ultrasonic water bath handles 1h, and obtained leaf of Moringa Thick many candies extracting solution is placed in 56 DEG C, and inspissation contracting is hanged under 800W, 3 times of volumes are added
Dehydrated alcohol precipitates overnight, be centrifuged, redissolve.Using complex enzyme formulation, (alkali protease: trypsase=8: 2) method takes off egg
It is white, it repeats 2-3 times, up to no white flock precipitate is precipitated.
(2) 20% alcohol precipitations
Dehydrated alcohol is added into the Polysaccharides from Leaves of Moringa oleifera solution after de- albumen, makes the volume 20% of dehydrated alcohol, alcohol precipitation at 4 DEG C
Overnight, it is centrifuged, removes supernatant, obtain 20% alcohol precipitation Polysaccharides from Leaves of Moringa oleifera extracting solution.
(3) anion exchange chromatography isolates and purifies
Polysaccharides from Leaves of Moringa oleifera extracting solution obtained in (2) step is redissolved, solution by DEAE-Sepharose Fast Flow from
Son exchange column chromatography for separation, is eluted with the NaCl solution of 0-1.0M, and eluent is concentrated through dialysis, and vacuum freeze drying obtains peppery
The wooden leaf polyose MPL20-1 powder.
2. Polysaccharides from Leaves of Moringa oleifera MLP20-1 described in claim 1 has preferable inhibitory activity to alpha-glucosidase, can be applied to drop
Hypoglycemic medicament and the preparation for treating diabetes, obesity drug.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710738449.1A CN109422818A (en) | 2017-08-21 | 2017-08-21 | A kind of preparation method and applications of Polysaccharides from Leaves of Moringa oleifera MLP20-1 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710738449.1A CN109422818A (en) | 2017-08-21 | 2017-08-21 | A kind of preparation method and applications of Polysaccharides from Leaves of Moringa oleifera MLP20-1 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109422818A true CN109422818A (en) | 2019-03-05 |
Family
ID=65500432
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710738449.1A Pending CN109422818A (en) | 2017-08-21 | 2017-08-21 | A kind of preparation method and applications of Polysaccharides from Leaves of Moringa oleifera MLP20-1 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109422818A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112457429A (en) * | 2020-12-30 | 2021-03-09 | 江西憨老俵实业发展有限公司 | Acetylated moringa oleifera leaf polysaccharide with hypoglycemic effect and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104829743A (en) * | 2015-05-25 | 2015-08-12 | 萧丽雅 | Preparation method and use of moringa oleifera leaf polysaccharides |
| CN104997817A (en) * | 2015-07-30 | 2015-10-28 | 太阳树（厦门）生物工程有限公司 | Extraction method of moringa oleifera leaf extractive |
| CN106474145A (en) * | 2016-11-29 | 2017-03-08 | 华南农业大学 | Application in preparation preventing and treating alcoholic liver injury medicine and food for the Polysaccharides from Leaves of Moringa oleifera |
| MX2015016653A (en) * | 2015-12-03 | 2017-06-02 | Univ Autónoma Metropolitana | Coagulation-flocculation kit for the treatment of residual waters based on moringa-chitosan. |
-
2017
- 2017-08-21 CN CN201710738449.1A patent/CN109422818A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104829743A (en) * | 2015-05-25 | 2015-08-12 | 萧丽雅 | Preparation method and use of moringa oleifera leaf polysaccharides |
| CN104997817A (en) * | 2015-07-30 | 2015-10-28 | 太阳树（厦门）生物工程有限公司 | Extraction method of moringa oleifera leaf extractive |
| MX2015016653A (en) * | 2015-12-03 | 2017-06-02 | Univ Autónoma Metropolitana | Coagulation-flocculation kit for the treatment of residual waters based on moringa-chitosan. |
| CN106474145A (en) * | 2016-11-29 | 2017-03-08 | 华南农业大学 | Application in preparation preventing and treating alcoholic liver injury medicine and food for the Polysaccharides from Leaves of Moringa oleifera |
Non-Patent Citations (2)
| Title |
|---|
| CHUN CHEN等: ""Microwave-assisted extraction of polysaccharides from Moringa oleifera Lam. leaves: Characterization and hypoglycemic activity"", 《INDUSTRIAL CROPS AND PRODUCTS》 * |
| 张恒主编: "《应用生物化学》", 31 January 2013, 中国矿业大学出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112457429A (en) * | 2020-12-30 | 2021-03-09 | 江西憨老俵实业发展有限公司 | Acetylated moringa oleifera leaf polysaccharide with hypoglycemic effect and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108477612B (en) | Dendrobium nutrition powder containing dendrobium officinale purified extract and preparation method thereof | |
| CN102988827B (en) | Dendrobium officinale granula | |
| CN102988828B (en) | Preparation method of dendrobium officinale granula | |
| CN101456918A (en) | Sealwort polysaccharide and preparation method thereof, and its application in preparing hypoglycemic and reducing blood lipid drugs | |
| Yampolsky et al. | Apricot (lat. Prunus armeniaca Lin.) | |
| CN104138526B (en) | A kind of root of three-nerved spicebush iron sheet ginseng granules agent | |
| CN106519054B (en) | A kind of radix pseudostellariae homogeneous polysaccharide and application thereof promoting Islet Cells Insulin secretion | |
| US20190000905A1 (en) | Gynostemma pentaphyllum fermented extracts comprising Saponins as an active functional food ingredient and preparation method thereof | |
| CN110755542A (en) | Formula of uric acid-reducing gout-treating tea and preparation method thereof | |
| Luan et al. | An update review of extraction, purification, structural features, bioactivities, and applications of Schisandra chinensis polysaccharides | |
| CN105147718A (en) | Application of mulberry polysaccharide extract in preparing medicine or health product | |
| Mittal et al. | A Critical Review on Ethnobotanical and Pharmacological Aspects of Euryale Ferox Salisb. | |
| CN109422818A (en) | A kind of preparation method and applications of Polysaccharides from Leaves of Moringa oleifera MLP20-1 | |
| CN106962910B (en) | Health-care composition for reducing blood pressure | |
| KR20130074119A (en) | Ginseng prosapogenin high concentration containing ginseng leaf or stem preparation using sonication and process for thereof | |
| CN113663043B (en) | Composition with alpha-glucosidase inhibition effect and preparation method and application thereof | |
| CN105192820A (en) | Blood-fat-reducing cordyceps militaris polysaccharide beverage and preparing method thereof | |
| CN111387394A (en) | Sea-buckthorn solid beverage for enhancing immunity and inhibiting tumors and preparation method thereof | |
| CN115192637A (en) | Daphniphyllum calycinum and polygonum hydropiper gastrointestinal health tablet and preparation process thereof | |
| CN110959856A (en) | Low-sugar tortoise jelly and preparation method thereof | |
| CN105030874A (en) | Preparation method of platycodon extract and extract and drink prepared by preparation method | |
| CN112076281A (en) | Ganoderma applanatum composition for enhancing immunity and preparation method thereof | |
| CN112245527A (en) | Preparation method of active polysaccharide extract, functional beverage and effervescent granule with homology of medicine and food | |
| CN101744996B (en) | Extraction method of ginseng, ophiopogon root and shiandra and preparation thereof | |
| CN110801469A (en) | Functional food for regulating blood sugar and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190305 |