CN109418050A - Method for artificially cultivating cistanche - Google Patents
Method for artificially cultivating cistanche Download PDFInfo
- Publication number
- CN109418050A CN109418050A CN201710735006.7A CN201710735006A CN109418050A CN 109418050 A CN109418050 A CN 109418050A CN 201710735006 A CN201710735006 A CN 201710735006A CN 109418050 A CN109418050 A CN 109418050A
- Authority
- CN
- China
- Prior art keywords
- parts
- seed
- cistanche
- treatment
- inoculation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000005787 Cistanche Species 0.000 title claims abstract description 69
- 238000000034 method Methods 0.000 title claims abstract description 56
- 238000011081 inoculation Methods 0.000 claims abstract description 50
- 241000196324 Embryophyta Species 0.000 claims abstract description 18
- 241000336291 Cistanche deserticola Species 0.000 claims description 61
- 239000003337 fertilizer Substances 0.000 claims description 45
- 150000001875 compounds Chemical class 0.000 claims description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 26
- 238000000855 fermentation Methods 0.000 claims description 23
- 230000004151 fermentation Effects 0.000 claims description 23
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 22
- 238000003756 stirring Methods 0.000 claims description 21
- 210000003608 fece Anatomy 0.000 claims description 20
- 239000002054 inoculum Substances 0.000 claims description 20
- 238000012258 culturing Methods 0.000 claims description 18
- -1 fluorine pyridine ketone Chemical class 0.000 claims description 18
- 238000012545 processing Methods 0.000 claims description 18
- 239000002994 raw material Substances 0.000 claims description 18
- 239000000463 material Substances 0.000 claims description 17
- NVGOPFQZYCNLDU-UHFFFAOYSA-N norflurazon Chemical compound O=C1C(Cl)=C(NC)C=NN1C1=CC=CC(C(F)(F)F)=C1 NVGOPFQZYCNLDU-UHFFFAOYSA-N 0.000 claims description 17
- 238000007654 immersion Methods 0.000 claims description 16
- 239000004927 clay Substances 0.000 claims description 15
- GUJOJGAPFQRJSV-UHFFFAOYSA-N dialuminum;dioxosilane;oxygen(2-);hydrate Chemical compound O.[O-2].[O-2].[O-2].[Al+3].[Al+3].O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O GUJOJGAPFQRJSV-UHFFFAOYSA-N 0.000 claims description 15
- 229910052901 montmorillonite Inorganic materials 0.000 claims description 15
- 239000000843 powder Substances 0.000 claims description 14
- OLBNOBQOQZRLMP-UHFFFAOYSA-N 2,6-dimethoxy-p-benzoquinone Chemical compound COC1=CC(=O)C=C(OC)C1=O OLBNOBQOQZRLMP-UHFFFAOYSA-N 0.000 claims description 13
- 230000002262 irrigation Effects 0.000 claims description 13
- 238000003973 irrigation Methods 0.000 claims description 13
- 239000001814 pectin Substances 0.000 claims description 13
- 235000010987 pectin Nutrition 0.000 claims description 13
- 229920001277 pectin Polymers 0.000 claims description 13
- 238000005138 cryopreservation Methods 0.000 claims description 12
- 241001494479 Pecora Species 0.000 claims description 11
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 11
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 claims description 11
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 11
- 239000004202 carbamide Substances 0.000 claims description 11
- 235000019691 monocalcium phosphate Nutrition 0.000 claims description 11
- 235000011009 potassium phosphates Nutrition 0.000 claims description 11
- 240000008042 Zea mays Species 0.000 claims description 10
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 10
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 10
- 235000005822 corn Nutrition 0.000 claims description 10
- 239000010871 livestock manure Substances 0.000 claims description 10
- 235000013312 flour Nutrition 0.000 claims description 9
- 238000004659 sterilization and disinfection Methods 0.000 claims description 9
- 238000003306 harvesting Methods 0.000 claims description 8
- 238000001035 drying Methods 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 7
- 229910000160 potassium phosphate Inorganic materials 0.000 claims description 7
- 239000000243 solution Substances 0.000 claims description 7
- 239000010902 straw Substances 0.000 claims description 7
- 235000013372 meat Nutrition 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 230000003447 ipsilateral effect Effects 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
- 239000002245 particle Substances 0.000 claims description 3
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims 1
- 239000011574 phosphorus Substances 0.000 claims 1
- 229910052698 phosphorus Inorganic materials 0.000 claims 1
- 239000011591 potassium Substances 0.000 claims 1
- 229910052700 potassium Inorganic materials 0.000 claims 1
- 210000002700 urine Anatomy 0.000 claims 1
- 230000012010 growth Effects 0.000 abstract description 9
- 230000035784 germination Effects 0.000 abstract description 8
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 7
- 230000003071 parasitic effect Effects 0.000 abstract description 7
- 230000024241 parasitism Effects 0.000 abstract description 6
- 230000005059 dormancy Effects 0.000 abstract description 2
- 230000004720 fertilization Effects 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 45
- 238000002474 experimental method Methods 0.000 description 19
- 239000005556 hormone Substances 0.000 description 7
- 229940088597 hormone Drugs 0.000 description 7
- 239000011259 mixed solution Substances 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
- 238000011160 research Methods 0.000 description 5
- 238000002255 vaccination Methods 0.000 description 5
- 238000003860 storage Methods 0.000 description 4
- 239000002585 base Substances 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 238000005457 optimization Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 238000003672 processing method Methods 0.000 description 2
- 239000002002 slurry Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000336316 Cistanche tubulosa Species 0.000 description 1
- 206010013786 Dry skin Diseases 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241001632080 Haloxylon Species 0.000 description 1
- 108010034145 Helminth Proteins Proteins 0.000 description 1
- 241000308150 Orobanchaceae Species 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 235000010185 Tamarix canariensis Nutrition 0.000 description 1
- 244000234281 Tamarix gallica Species 0.000 description 1
- 235000014265 Tamarix gallica Nutrition 0.000 description 1
- 235000010154 Tamarix ramosissima Nutrition 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 150000004054 benzoquinones Chemical class 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 235000013877 carbamide Nutrition 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 230000002124 endocrine Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 244000000013 helminth Species 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- MPNNOLHYOHFJKL-UHFFFAOYSA-N peroxyphosphoric acid Chemical compound OOP(O)(O)=O MPNNOLHYOHFJKL-UHFFFAOYSA-N 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 235000020995 raw meat Nutrition 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/04—Arranging seed on carriers, e.g. on tapes, on cords ; Carrier compositions
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/06—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinone; Derivatives thereof, e.g. ketals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/58—1,2-Diazines; Hydrogenated 1,2-diazines
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Soil Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Fertilizers (AREA)
Abstract
The invention provides a method for artificially cultivating cistanche, belonging to the field of plant cultivation. The invention comprises the following steps: in the method, the cistanche is subjected to temperature circulation and chemical reagent treatment, so that the physiological dormancy of the cistanche seeds can be broken, the germination of the seeds is promoted, and the parasitic rate is improved; meanwhile, the success rate and the yield of cistanche inoculation are further improved by further selecting, adjusting and optimizing the treatment temperature condition and the used chemical reagent; in addition, the treatment method and the treatment reagent which are specifically adopted in the steps of seed treatment, seed inoculation, late fertilization culture and the like are selected, adjusted and optimized, so that the germination, parasitism and growth of the cistanche seeds are effectively promoted, and the method has important practical significance for the expanded cultivation of cistanche.
Description
Technical field
The present invention relates to field of plant cultivation, in particular to a kind of method of artificial culture Herba Cistanches.
Background technique
Herba Cistanches (scientific name Cistanche deserticola Ma), alias boundary rue, very little rue, desert cistanche are Orobanchaceae meat desert
The parasitic plant that Rong belongs to, mainly has Desert Herba Cistanches (Haloxylon), Cistanche tubulosa (Tamarix), salt raw meat desert with host's difference
Rong (alkali fluffy etc.) three classes.Main product has fabulous in the Xinjiang in China, the desert or salt-soda soil of one band of the Inner Mongol, Gansu and Ningxia
Medical value, be known as the title of " desert ginseng ", from ancient times as civil excellent tonic product.
In recent years, research extensively and profoundly has all been carried out to Herba Cistanches chemical component, pharmacological action both at home and abroad, as a result table
It is bright, Herba Cistanches enhancing body immunity, improve human body endurance, tonifying kidney and strengthening yang, increase intelligence, liver protection, adjust endocrine and it is anti-oxidant,
Anti-aging etc. shows certain activity.With the improvement of people's living standards, Herba Cistanches are increasingly taken seriously, market
It is in great demand.Currently, China Herba Cistanches come from wild resource substantially, since transition is excavated, Herba Cistanches resource is caused to be on the verge of withered
It exhausts, has been cited as China national second class protection plant, and be incorporated into " international conservation of wild plant register ".Present Wild Cistanche
Oneself is prohibited to excavate.
Most of Herba Cistanches all produce under field conditions (factors), its rate of vaccination is only 7/1000ths.From last century 80
In the age, oneself has carried out extensive experimentation research to artificial culture Herba Cistanches at home, achieves many technical experiences, but deposit always
The problems such as lower in rate of vaccination, yield is unstable, inoculation, harvesting higher cost, the complete of industrialization can be reached by not yet being formed
Kind production technology mode.Although Herba Cistanches exploitation is promoted and is helped as local new industry by some areas,
Effect is not satisfactory.
In view of this, the present invention is specifically proposed.
Summary of the invention
The first object of the present invention is to provide a kind of method of artificial culture Herba Cistanches, and the method has Herba Cistanches kind
The advantages that sub- activity is good, and success ratio of inoculation is high and Herba Cistanches yield is high.
In order to realize above-mentioned purpose of the invention, the following technical scheme is adopted:
A kind of method of artificial culture Herba Cistanches, described method includes following steps:
(1) seed treatment:
After fresh acquisition and/or the cistanche deserticola seed disinfection treatment of cryo-conservation, be placed in temperature be 55~60 DEG C, it is opposite
In the culturing room that humidity is 40~60%, and place 2~3h；Then cistanche deserticola seed is taken out from culturing room, is placed in temperature
In the culturing room that degree is 5~10 DEG C, relative humidity is 20~30%, and place 48~72h；And circular treatment 3-4 times；
By treated, cistanche deserticola seed carries out immersion treatment, and the time of processing is 3~5h, and after immersion treatment, will
The In Shade natural drying of cistanche deserticola seed；
Wherein, solution used in immersion treatment be dissolved with norflurazon, fluorine pyridine ketone and 2,6- dimethoxy 1,4-benzoquinone it is water-soluble
Liquid；
Wherein, the concentration of norflurazon is 1 × 10-9~1 × 10-5Mol/L, the concentration of fluorine pyridine ketone are 1 × 10-9~1 × 10- 6Mol/L, the concentration of 2,6- dimethoxy 1,4-benzoquinone are 1 × 10-5~1 × 10-8mol/L；
(2) it is inoculated with
Suitable quantity of water is added into clay and stirs into mud；Pentosan and pectin are added in suitable quantity of water and stirred evenly, and mud is added
In slurry, montmorillonite is then added and continues to stir evenly to form Inoculant；
Inoculant is uniformly applied to base stock surface, obtains inoculating paper；Then uniformly paving spills natural drying on inoculating paper
Seed, is then compacted by cistanche deserticola seed afterwards, and dries；
Inoculation ditch is excavated by red building, inoculating paper is placed in bottom of trench, and red building root is made to be in the top of inoculating paper, then
It is backfilled；
(3) fertilizer irrigation
It is ipsilateral in inoculation ditch after inoculation, it is applied fertilizer using compound fertilizer to red building, and poured after fertilising；
Wherein, the compound fertilizer is mainly prepared from the following raw materials: dry cow dung, dry sheep manure, plant ash, corn flour, stalk
Powder, urea, potassium phosphate and calcium superphosphate.
Optionally, in the present invention, norflurazon, fluorine pyridine ketone and 2, the molar concentration rate of 6- dimethoxy 1,4-benzoquinone be (1~
3): (2~5): (1~4).
Optionally, in the present invention, pentosan, pectin, montmorillonite and clay mass ratio be (5~15): (0.1~0.2):
(3~5): (20~25).
Optionally, in the present invention, the length of inoculating paper is 20~25cm, width is 8~10cm, Inoculant with a thickness of 3
~5mm.
Optionally, in the present invention, in the compound fertilizer, in parts by weight, each material quality number is as follows: dry ox
40~50 parts of excrement, 18~20 parts of dry sheep manure, 5~10 parts of plant ash, 10~15 parts of corn flour, 8~10 parts of straw powder, urea 5~
10 parts, 3~5 parts of potassium phosphate and 3~5 parts of calcium superphosphate.
Optionally, in the present invention, in the compound fertilizer, according to mass fraction meter, each material quality number is as follows: dry ox
50 parts of excrement, 20 parts of dry sheep manure, 5 parts of plant ash, 10 parts of corn flour, 8 parts of straw powder, 5 parts of urea, 5 parts of potassium phosphate and peroxophosphoric acid
5 parts of calcium.
Optionally, in the present invention, the preparation method of the compound fertilizer includes the following steps: after mixing each raw material, will
Compound powder is broken into 80~100 mesh particles, is then again stirring for, pours into fermentation vat after stirring, and water is added, so that material
Humidity is 50~60%, and it is in heaps then to stir and evenly mix heap, and ferment 24~36h；Then, turning is carried out, is added again after turning suitable
Water is measured, and makes the humidity of material be 40~50%, and 48~60h of heap fermentation in heaps；Turning again after fermentation, and be added appropriate
Water, so that the humidity of material is 30~45%, heap fermentation 72~84h, as compound fertilizer in heaps again.
Optionally, in the present invention, the method may further include the step of carrying out top dressing in fertilising 1 year.
Optionally, in the present invention, the method still further comprises the step of harvesting Herba Cistanches.
Compared with prior art, the invention has the benefit that
(1) in the present invention, by being handled using temperature and chemical reagent Herba Cistanches, so as to break Herba Cistanches kind
The physiological dormancy of son promotes germination, and improves parasitic rate；Meanwhile by treatment temperature condition and used in the present invention
Further selection, adjustment and the optimization of chemical reagent, can further increase the success rate of inoculation；
(2) in the present invention, by the selection and dosage adjustment and optimization to Inoculant raw material, it can not only effectively improve and connect
The bond properties of kind agent, while it is also possible that Inoculant has good water suction and water retention property, and then raising Herba Cistanches connect
Kind rate；
(3) in the present invention, by the selection and adjustment to host's red building fertilizer material, so as to remarkably promote Herba Cistanches
The growth of host red building, and then improve the yield of Herba Cistanches.
Specific embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the invention.It is not specified in embodiment specific
Condition person carries out according to conventional conditions or manufacturer's recommended conditions.Reagents or instruments used without specified manufacturer is
The conventional products that can be obtained by commercially available purchase.
Although in the prior art, having had more research to the cultivation of Herba Cistanches, these research be all from
Single aspect is set out, and is seldom related to Herba Cistanches and is sprouted from seed, to the entire incubation of parasitic and late growth, right
The research of connection and linking between each cultivation stage is even more fewer and fewer.
The present invention from the overall flow of cultivation, grind by the entirety for having carried out Herba Cistanches from seed treatment to recovery process
Study carefully and programme planning, each stage of cultivation be subjected to effective linking, effectively raise Herba Cistanches rate of vaccination and into
The yield of one step, specific:
Firstly, being that Herba Cistanches carry out seed treatment, since Herba Cistanches are a kind of parasitic plants, thus pre-processed
The suspend mode of seed can be broken with stimulation, and then haustorium is developed, completed parasitic；
The method of physics and chemistry can stimulate Herba Cistanches to make its sprouting, but single method effect is not ten clearly demarcated
It is aobvious, thus, the method associated with physical-chemical that uses in the application breaks the suspend mode of cistanche deserticola seed, and then can effectively mention
The success rate of height inoculation；
The specific steps of seed treatment are as follows: after fresh acquisition and/or the cistanche deserticola seed disinfection treatment of cryo-conservation, set
In the culturing room that temperature is 55~60 DEG C, relative humidity is 40~60%, and place 2~3h；Then by cistanche deserticola seed from
It takes out, is placed in the culturing room that temperature is 5~10 DEG C, relative humidity is 20~30% in culturing room, and place 48~72h；
And circular treatment 3-4 times；Treated cistanche deserticola seed is dissolved with norflurazon, fluorine pyridine ketone and 2,6- dimethoxy 1,4-benzoquinone
Aqueous solution carries out immersion treatment, and the time of processing is 3~5h, and after immersion treatment, and cistanche deserticola seed is In Shade certainly
It is so dry；
Preferably, the disinfection treatment includes the following steps: cistanche deserticola seed with 50% alcohol solution dipping 1-2min
Afterwards, then with the liquor natrii hypochloritis of 1-2% 10min is sterilized, finally with distilled water flushing, that is, completes disinfection；
Preferably, in the present invention, the concentration of norflurazon used is 1 × 10-6~1 × 10-7The concentration of mol/L, fluorine pyridine ketone is
1×10-6~1 × 10-7Mol/L, the concentration of 2,6- dimethoxy 1,4-benzoquinone are 1 × 10-6~1 × 10-7mol/L；It is furthermore preferred that
The molar concentration rate of norflurazon, fluorine pyridine ketone and 2,6- dimethoxy 1,4-benzoquinone is 1:3:3；
Then, it is inoculated with, the method that the present invention is inoculated with using inoculating paper, since Herba Cistanches growth district arid is few
Rain, it is then desired to be effectively retained in inoculation region.The pentosan that the present invention is added can not only effectively improve inoculation
The adhesion strength of paper, while can also play the role of water conservation, while can also grow for red building and certain nutriment is provided；And
The use of montmorillonite can also be sprouted for cistanche deserticola seed using its good water absorbing properties and provide sufficient ambient moisture.
Specifically, the inoculation step is as follows: suitable quantity of water is added into clay and stirs into mud；Pentosan and pectin are added
Enter in suitable quantity of water and stir evenly, and be added in mud, montmorillonite is then added and continues to stir evenly to form Inoculant；Inoculant is uniformly smeared
In base stock surface, inoculating paper is obtained；Then uniformly paving spills the cistanche deserticola seed after spontaneously drying on inoculating paper, then by seed
Compacting, and dry；Inoculation ditch is excavated by red building, inoculating paper is placed in bottom of trench, and red building root is made to be in the top of inoculating paper,
Then it is backfilled；
Preferably, in the present invention, pentosan, pectin, montmorillonite and clay mass ratio be (5~15): (0.1~0.2):
(3~5): (20~25)；It is furthermore preferred that in the present invention, pentosan, pectin, montmorillonite and clay mass ratio be 5:0.1:3:
25。
Then, it is exactly fertilising after inoculation, after Herba Cistanches are parasitic, nutrition needed for growing and moisture are required from host's body
Upper acquisition, thus, host plant growing state also can largely influence the yield of Herba Cistanches；
In order to further promote the growth of host plant, the compound fertilizer of suitable red building growth is additionally provided in the method for the present invention
Material, can apply fertilizer after inoculation, the place of fertilising be selected in inoculation ditch it is ipsilateral, with inoculation ditch distance controlling 1m with
It is interior；
The compound fertilizer raw material is as follows: 40~50 parts of dry cow dung, 18~20 parts of dry sheep manure, 5~10 parts of plant ash, corn
10~15 parts of powder, 8~10 parts of straw powder, 5~10 parts of urea, 3~5 parts of potassium phosphate and 3~5 parts of calcium superphosphate, the fertilizer
Based on agriculture organic matter, and it is aided with trace-element fertilizer needed for red building grows, not only safety and environmental protection, but also can effectively promotees
Into the growth of red building；
Further, after the compound fertilizer is the preparation method is as follows: each raw material is mixed, compound powder is broken into 80~
100 mesh particles, are then again stirring for, pour into fermentation vat after stirring, and water is added, so that the humidity of material is 50~60%,
Then it is in heaps to stir and evenly mix heap, ferment 24~36h；Then, turning is carried out, suitable quantity of water is added after turning again, and make material
Humidity be 40~50%, and 48~60h of heap fermentation in heaps；Turning again after fermentation, and suitable quantity of water is added, so that material is wet
Degree is 30~45%, again heap fermentation 72~84h, as compound fertilizer in heaps；And the method for using fermentation can not only be killed
The helminth and germ that may be mixed in raw material, while can also be by raw material decomposes by microbial fermentation effect can be by red building
It absorbs and the minerals that utilize and other nutritional ingredients.
Finally, the present invention still further comprises the step of harvesting Herba Cistanches, Herba Cistanches can be carried out after inoculation 2 years
Harvesting, Herba Cistanches are broken ground Post flowering, can be harvested when seed is scattered when begin under seed blackening, flower spike, on the ground by Herba Cistanches
Part is cut, and is then being dried in the cool, takes out seed, and seed is saved at low temperature.
Embodiment 1
After the cistanche deserticola seed of cryo-conservation is disinfected, it is placed in the culture that temperature is 57 DEG C, relative humidity is 50%
In room, and place 3h；Then cistanche deserticola seed is taken out from culturing room, is placed in that temperature is 8 DEG C, relative humidity is 25%
In culturing room, and 72h is placed, and circular treatment 3 times；
Aqueous solution is configured, and the concentration of norflurazon in solution is made to be 1 × 10-7Mol/L, the concentration of fluorine pyridine ketone are 3 × 10-7Mol/L, the concentration of 2,6- dimethoxy 1,4-benzoquinone are 3 × 10-7mol/L；
Then, treated cistanche deserticola seed carries out immersion treatment with the aqueous solution, and the time of processing is 4h, and is being impregnated
After processing, by the In Shade natural drying of cistanche deserticola seed；
Suitable quantity of water is added into clay and stirs into mud；Pentosan and pectin are added in suitable quantity of water and stirred evenly, and mud is added
In slurry, montmorillonite is then added and continues to stir evenly to form Inoculant；And make the mass ratio of pentosan, pectin, montmorillonite and clay
For 5:0.1:3:25
Inoculant is uniformly applied to the base stock surface that length is 20cm, width is 10cm, and makes the thickness of Inoculant
For 3mm, as inoculating paper；Then uniformly paving spills the cistanche deserticola seed after 100 natural dryings on inoculating paper, then by seed
Compacting, and dry；
Inoculation ditch is excavated by red building, inoculating paper is placed in bottom of trench, and red building root is made to be in the top of inoculating paper, then
It is backfilled；
It is ipsilateral in inoculation ditch after inoculation, it is applied fertilizer using compound fertilizer to red building, and poured after fertilising；
Wherein, the compound fertilizer is the preparation method is as follows: by 50 parts of dry cow dungs, 20 parts of dry sheep manures, 5 parts of plant ash, 10 parts
After corn flour, 8 parts of straw powders, 5 parts of urea, 5 parts of potassium phosphates and 5 parts of calcium superphosphate mixing, compound powder is broken into 90 mesh
Grain, is then again stirring for, pours into fermentation vat after stirring, and water is added, so that the humidity of material is 50%, then stirs and evenly mixs
Heap is in heaps, and ferment 36h；Then, turning is carried out, suitable quantity of water is added after turning again, and makes the humidity of material be 50%, and heap
Fermentation 48h in heaps；Turning again after fermentation, and suitable quantity of water is added, so that the humidity of material is 45%, heap fermentation in heaps again
72h。
It can carry out the harvestings of Herba Cistanches after inoculation 2 years, Herba Cistanches are broken ground Post flowering, to open under seed blackening, flower spike
Beginning can harvest when having seed to be scattered, and Herba Cistanches aerial part is cut, is then being dried in the cool, take out seed, and will
Seed saves at low temperature.
Experimental example 1
(1) influence that Temperature Treatment sprouts cistanche deserticola seed
According to the method for embodiment 1, to the cistanche deserticola seed of cryo-conservation (holding time is 2 years, and storage temperature is 4 DEG C)
Temperature cycles processing and HORMONE TREATMENT are carried out, 1 group of experiment is denoted as；
According to the method for embodiment 1, circulating temperature and HORMONE TREATMENT are carried out to the seed in 1 group of experiment with batch, are denoted as
Test 2 groups；Wherein, it tests in 2 groups, the number of temperature cycles is 1 time；
According to the method for embodiment 1, temperature cycles and HORMONE TREATMENT are carried out to the seed in 1 group of experiment with batch, are denoted as
Test 3 groups；Wherein, it tests in 3 groups, the number of temperature cycles is 6 times；
After in experimental group 1 with the seed disinfection processing of batch, it is placed in the culture that temperature is 50 DEG C, relative humidity is 50%
3h is placed in room；Then, cistanche deserticola seed is taken out from culturing room, is placed in the training that temperature is 3 DEG C, relative humidity is 30%
It supports in room and places 72h, and circular treatment 3 times, be denoted as 4 groups of experiment；
After in experimental group 1 with the seed disinfection processing of batch, it is placed in the culture that temperature is 35 DEG C, relative humidity is 50%
3h is placed in room；Then, cistanche deserticola seed is taken out from culturing room, is placed in that temperature is 10 DEG C, relative humidity is 30%
72h, and circular treatment 3 times are placed in culturing room, are denoted as 5 groups of experiment；
After in experimental group 1 with the seed disinfection processing of batch, it is placed in the culture that temperature is 75 DEG C, relative humidity is 60%
In room, and place 3h；Then cistanche deserticola seed is taken out from culturing room, be placed in temperature be 20 DEG C, relative humidity 20%
Culturing room in place 72h, and circular treatment 3 times, be denoted as experiment 6 groups；
After in experimental group 1 with the seed disinfection processing of batch, it is placed in the culture that temperature is 75 DEG C, relative humidity is 60%
In room, and place 3h；Then cistanche deserticola seed is taken out from culturing room, is placed in that temperature is 5 DEG C, relative humidity is 20%
72h, and circular treatment 3 times are placed in culturing room, are denoted as 7 groups of experiment；
By each experimental group, treated that cistanche deserticola seed is placed in sterile petri dish, and sterile distilled water, juxtaposition is then added
It is cultivated under 24 DEG C of dark conditions；With then, seed sprouting situation is observed, initial seed sprout time is recorded, to 15 days
After count germination rate；As a result as shown in the table:
| Experimental group | Sprout time (day) | Germination rate (%) |
| Test 1 group | 6 | 90 |
| Test 2 groups | 8 | 67 |
| Test 3 groups | 7 | 83 |
| Test 4 groups | 8 | 85 |
| Test 5 groups | 8 | 65 |
| Test 6 groups | 9 | 52 |
| Test 7 groups | 9 | 61 |
The influence that temperature cycles processing step sprouts cistanche deserticola seed it can be seen from the contrasting data of upper table compared with
Greatly, the control either in cycle-index or cyclic process to temperature condition all plays more the induction sprouting of Herba Cistanches
Important role.And induced using the method for the present invention, it can be improved the germination rate of Herba Cistanches, and shorten sprout time.
(2) influence that HORMONE TREATMENT sprouts cistanche deserticola seed
According to 1 method of embodiment to cryo-conservation (holding time be 2 years, storage temperature be 4 DEG C) cistanche deserticola seed into
Trip temperature circulation and HORMONE TREATMENT, are denoted as 1 group of experiment；
After embodiment 1 is sterilized with batch cistanche deserticola seed, only with temperature cycles processing method (with item in embodiment 1
Part is identical) cistanche deserticola seed is handled, it is denoted as 2 groups of experiment；
After embodiment 1 is sterilized with batch cistanche deserticola seed, use concentration for 7 × 10-6Mol/L norflurazon solution is to meat
Desert cistanche seed carries out immersion treatment, is denoted as 3 groups of experiment；
After embodiment 1 is sterilized with batch cistanche deserticola seed, use concentration for 7 × 10-6Mol/L fluorine pyridine ketone solution is to meat
Desert cistanche seed carries out immersion treatment, is denoted as 4 groups of experiment；
After embodiment 1 is sterilized with batch cistanche deserticola seed, use concentration for 7 × 10-6Mol/L 2,6- dimethoxy pair
Benzoquinones solution carries out immersion treatment to cistanche deserticola seed, is denoted as 5 groups of experiment；
After embodiment 1 is sterilized with batch cistanche deserticola seed, use concentration for 3.5 × 10-6Mol/L norflurazon, 3.5 ×
10-7The mixed solution of mol/L fluorine pyridine ketone carries out immersion treatment to cistanche deserticola seed, is denoted as 6 groups of experiment；
After embodiment 1 is sterilized with batch cistanche deserticola seed, use concentration for 3.5 × 10-6Mol/L norflurazon, 3.5 ×
10-7The mixed solution of mol/L2,6- dimethoxy 1,4-benzoquinone carries out immersion treatment to cistanche deserticola seed, is denoted as 7 groups of experiment；
After embodiment 1 is sterilized with batch cistanche deserticola seed, use concentration for 3.5 × 10-6Mol/L fluorine pyridine ketone, 3.5 ×
10-7The mixed solution of mol/L 2,6- dimethoxy 1,4-benzoquinone carries out immersion treatment to cistanche deserticola seed, is denoted as 8 groups of experiment；
After embodiment 1 is sterilized with batch cistanche deserticola seed, use concentration for 3 × 10-7Mol/L norflurazon, 2 × 10- 7Mol/L fluorine pyridine ketone and 2 × 10-7Mol/L 2,6- dimethoxy 1,4-benzoquinone mixed solution carries out at immersion cistanche deserticola seed
Reason is denoted as 9 groups of experiment；
After embodiment 1 is sterilized with batch cistanche deserticola seed, use concentration for 2 × 10-7Mol/L norflurazon, 4 × 10- 7Mol/L fluorine pyridine ketone and 1 × 10-7The mixed solution of mol/L 2,6- dimethoxy 1,4-benzoquinone impregnates cistanche deserticola seed
Processing is denoted as 10 groups of experiment；
After embodiment 1 is sterilized with batch cistanche deserticola seed, use concentration for 2 × 10-7Mol/L norflurazon, 5 × 10- 7Mol/L fluorine pyridine ketone and 2 × 10-7The mixed solution of mol/L 2,6- dimethoxy 1,4-benzoquinone impregnates cistanche deserticola seed
Processing is denoted as 11 groups of experiment；
After embodiment 1 is sterilized with batch cistanche deserticola seed, use concentration for 1 × 10-7Mol/L norflurazon, 2 × 10- 7Mol/L fluorine pyridine ketone and 1 × 10-7The mixed solution of mol/L 2,6- dimethoxy 1,4-benzoquinone impregnates cistanche deserticola seed
Processing is denoted as 12 groups of experiment；
By respectively treated, experimental group cistanche deserticola seed is placed in sterile petri dish, and sterile distilled water, juxtaposition is then added
It is cultivated under 24 DEG C of dark conditions；With then, seed sprouting situation is observed, initial seed sprout time is recorded, to 15 days
After count germination rate；As a result as shown in the table:
| Experimental group | Sprout time (day) | Germination rate (%) |
| Test 1 group | 6 | 90 |
| Test 2 groups | 12 | 7 |
| Test 3 groups | 8 | 57 |
| Test 4 groups | 8 | 46 |
| Test 5 groups | 10 | 28 |
| Test 6 groups | 9 | 56 |
| Test 7 groups | 8 | 73 |
| Test 8 groups | 9 | 76 |
| Test 9 groups | 7 | 83 |
| Test 10 groups | 7 | 77 |
| Test 11 groups | 6 | 85 |
| Test 12 groups | 9 | 72 |
By above-mentioned Experimental comparison's data it is found that hormon proportion and different hormone kinds have Herba Cistanches sprouting
Large effect three kinds of hormones are used in combination, sprouting for Herba Cistanches can be greatly improved and according in the method for the invention
Hair, and this is also beneficial to its further haustorium development and parasitism, and then improves success ratio of inoculation.
(3) influence of the Inoculant to Herba Cistanches rate of vaccination
(holding time is 2 years to cistanche deserticola seed according to 1 the method for embodiment to cryo-conservation, storage temperature 4
DEG C) handled, then in spring, inoculation and fertilizer irrigation is carried out according to 1 method of embodiment, and carry out top dressing after 1 year, remembered
For test group 1, and success ratio of inoculation is investigated after 2 years；
Seed treatment and inoculation and fertilizer irrigation and top dressing are carried out according to the identical method of experimental group 1, is denoted as experimental group
2；Wherein, in experimental group 2, Inoculant used is the mixture of pentosan, pectin and clay, and the mass ratio of three is 5:0.1:
25；
Seed treatment and inoculation and fertilizer irrigation and top dressing are carried out according to the identical method of experimental group 1, is denoted as experimental group
3；Wherein, in experimental group 3, Inoculant used is the mixture of pentosan, montmorillonite and clay, and the mass ratio of three is 5:3:
25；
Seed treatment and inoculation and fertilizer irrigation and top dressing are carried out according to the identical method of experimental group 1, is denoted as experimental group
4；Wherein, in experimental group 4, Inoculant used is the mixture of sucrose, montmorillonite and clay, and the mass ratio of three is 5:3:
25；
Seed treatment and inoculation and fertilizer irrigation and top dressing are carried out according to the identical method of experimental group 1, is denoted as experimental group
5；Wherein, in experimental group 5, Inoculant used be pentosan, pectin, montmorillonite and clay mixture, the mass ratio of three
For 1:1:1:5；
Each experimental group success ratio of inoculation result is as follows:
| Experimental group | Success ratio of inoculation (%) |
| Test group 1 | 83 |
| Test group 2 | 47 |
| Test group 3 | 71 |
| Test group 4 | 63 |
| Test group 5 | 76 |
By the data of upper table it is found that the difference of inoculant formula and raw material proportioning also have for success ratio of inoculation it is biggish
It influences, and it is in the present invention that pentosan, pectin, montmorillonite and clay is mixed, and to prepared after the proportion adjustment of each raw material dosage
Inoculant, can effectively improve Herba Cistanches parasitism inoculation success rate.
(4) influence of the fertilizer irrigation to Herba Cistanches rate of vaccination
(holding time is 2 years to cistanche deserticola seed according to 1 the method for embodiment to cryo-conservation, storage temperature 4
DEG C) handled, then in spring, inoculation and fertilizer irrigation is carried out according to 1 method of embodiment, and carry out top dressing after 1 year, remembered
For test group 1, and success ratio of inoculation is investigated after 2 years；
It is carried out according to cistanche deserticola seed of 1 the method for embodiment to cryo-conservation is (identical as 1 seed batch of test group)
Processing carries out inoculation and fertilizer irrigation according to 1 method of embodiment, and carry out top dressing after 1 year, is denoted as test then in spring
Group 2, and success ratio of inoculation is investigated after 2 years；Wherein, in experimental group 2 compound fertilizer fermentation raw material are as follows: 50 parts of dry cow dungs, 40
Part dry sheep manure, 15 parts of urea, 5 parts of potassium phosphates and 10 parts of calcium superphosphate；
It is carried out according to cistanche deserticola seed of 1 the method for embodiment to cryo-conservation is (identical as 1 seed batch of test group)
Processing carries out inoculation and fertilizer irrigation according to 1 method of embodiment, and carry out top dressing after 1 year, is denoted as test then in spring
Group 3, and success ratio of inoculation is investigated after 2 years；Wherein, in experimental group 3 compound fertilizer fermentation raw material are as follows: 100 parts of dry cow dungs, 5
Part plant ash, 10 parts of stalk parts, 10 parts of urea, 5 parts of potassium phosphates and 10 parts of calcium superphosphate；
It is carried out according to cistanche deserticola seed of 1 the method for embodiment to cryo-conservation is (identical as 1 seed batch of test group)
Processing carries out inoculation and fertilizer irrigation according to 1 method of embodiment, and carry out top dressing after 1 year, is denoted as test then in spring
Group 4, and success ratio of inoculation is investigated after 2 years；Wherein, in experimental group 4 compound fertilizer fermentation raw material are as follows: 100 parts of dry sheep manures, 10
Part corn flour, 10 parts of stalk parts, 10 parts of urea, 5 parts of potassium phosphates and 10 parts of calcium superphosphate；
It is carried out according to cistanche deserticola seed of 1 the method for embodiment to cryo-conservation is (identical as 1 seed batch of test group)
Processing carries out inoculation and fertilizer irrigation according to 1 method of embodiment, and carry out top dressing after 1 year, is denoted as test then in spring
Group 5, and success ratio of inoculation is investigated after 2 years；Wherein, in experimental group 5 compound fertilizer fermentation raw material are as follows: it is 10 parts of dry cow dung, dry
10 parts of sheep dung, 10 parts of plant ash, 20 parts of corn flour, 30 parts of straw powder, 5 parts of urea, 5 parts of potassium phosphate and 5 parts of calcium superphosphate；
Each experimental group success ratio of inoculation result is as follows:
| Experimental group | Success ratio of inoculation (%) |
| Test group 1 | 83 |
| Test group 2 | 75 |
| Test group 3 | 67 |
| Test group 4 | 73 |
| Test group 5 | 57 |
It is weighed after by the harvesting of experimental group Herba Cistanches, the results showed that, the per mu yield highest of test group 1, the per mu yield of experimental group 5
It measures minimum.The Herba Cistanches fresh weight per mu of experimental group 5 can reach 300Kg or so；The Herba Cistanches fresh weight per mu of experimental group 1 is test group
1.3 times or so of 5；The Herba Cistanches fresh weight per mu of experimental group 2 is 1.1 times or so of experimental group 5；The Herba Cistanches per mu of experimental group 3
Fresh weight is 1.2 times or so of experimental group 5, and the Herba Cistanches fresh weight per mu of experimental group 4 is slightly above experimental group 5.
It can be seen that fertilizer used in the later period has the rate of successful parasitism and growth rate of Herba Cistanches after Herba Cistanches inoculation
More important influence, and the fertilizer that the present invention is prepared by the cooperation fermentation of the raw material of specific Organic-inorganic fertilizer, for meat
The parasitism of desert cistanche and the facilitation of growth are best.
In the present invention, by from seed treatment and seed inoculation and the later period fertilising culture and etc. in specifically use
Processing method and reagent treatment selection, adjustment and optimization, effectively promoted sprouting, parasitism and the life of cistanche deserticola seed
Long, this has important practical significance for Herba Cistanches expand and cultivate.
Although illustrate and describing the present invention with specific embodiment, it will be appreciated that without departing substantially from of the invention
Many other change and modification can be made in the case where spirit and scope.It is, therefore, intended that in the following claims
Including belonging to all such changes and modifications in the scope of the invention.
Claims (10)
1. a kind of method of artificial culture Herba Cistanches, which is characterized in that described method includes following steps:
(1) seed treatment:
After fresh acquisition and/or the cistanche deserticola seed disinfection treatment of cryo-conservation, being placed in temperature is 55~60 DEG C, relative humidity
For in 40~60% culturing room, and place 2~3h；Then cistanche deserticola seed is taken out from culturing room, being placed in temperature is 5
~10 DEG C, relative humidity be 20~30% culturing room in, and place 48~72h；And circular treatment 3-4 times；
By treated, cistanche deserticola seed carries out immersion treatment, and the time of processing is 3~5h, and after immersion treatment, by meat desert
The In Shade natural drying of Rong's seed；
Wherein, solution used in immersion treatment is dissolved with norflurazon, fluorine pyridine ketone and 2, the aqueous solution of 6- dimethoxy 1,4-benzoquinone；
Wherein, the concentration of norflurazon is 1 × 10-9~1 × 10-5Mol/L, the concentration of fluorine pyridine ketone are 1 × 10-9~1 × 10-6mol/
L, the concentration of 2,6- dimethoxy 1,4-benzoquinone are 1 × 10-5~1 × 10-8mol/L；
(2) it is inoculated with
Suitable quantity of water is added into clay and stirs into mud；Pentosan and pectin are added in suitable quantity of water and stirred evenly, and is added in mud,
Then montmorillonite is added to continue to stir evenly to form Inoculant；
Inoculant is uniformly applied to base stock surface, obtains inoculating paper；Then after uniformly paving spills natural drying on inoculating paper
Seed is then compacted by cistanche deserticola seed, and is dried；
Inoculation ditch is excavated by red building, inoculating paper is placed in bottom of trench, and red building root is made to be in the top of inoculating paper, then carried out
Backfill；
(3) fertilizer irrigation
It is ipsilateral in inoculation ditch after inoculation, it is applied fertilizer using compound fertilizer to red building, and poured after fertilising；
Wherein, the compound fertilizer is mainly prepared from the following raw materials: dry cow dung, dry sheep manure, plant ash, corn flour, straw powder, urine
Element, potassium phosphate and calcium superphosphate.
2. the method according to claim 1, wherein norflurazon, fluorine pyridine ketone and 2,6- dimethoxy 1,4-benzoquinone
Molar concentration rate be (1~3): (2~5): (1~4).
3. the method according to claim 1, wherein pentosan, pectin, montmorillonite and clay mass ratio be (5
~15): (0.1~0.2): (3~5): (20~25).
4. according to the method described in claim 3, it is characterized in that, pentosan, pectin, montmorillonite and clay mass ratio are as follows:
5:0.1:3:25.
5. the method according to claim 1, wherein the length of inoculating paper is 20~25cm, width be 8~
10cm, Inoculant with a thickness of 3~5mm.
6. the method according to claim 1, wherein in the compound fertilizer, in parts by weight, each raw material
Mass fraction is as follows: 40~50 parts of dry cow dung, 18~20 parts of dry sheep manure, 5~10 parts of plant ash, 10~15 parts of corn flour, stalk
8~10 parts of powder, 5~10 parts of urea, 3~5 parts of potassium phosphate and 3~5 parts of calcium superphosphate.
7. according to the method described in claim 6, it is characterized in that, in the compound fertilizer, according to mass fraction meter, each raw material
Mass fraction is as follows: 50 parts of dry cow dung, 20 parts of dry sheep manure, 5 parts of plant ash, 10 parts of corn flour, 8 parts of straw powder, 5 parts of urea, phosphorus
5 parts of sour potassium and 5 parts of calcium superphosphate.
8. according to the method described in claim 6, it is characterized in that, the preparation method of the compound fertilizer includes the following steps:
After each raw material is mixed, compound powder is broken into 80~100 mesh particles, is then again stirring for, is poured into fermentation vat after stirring, and
Water is added, so that the humidity of material is 50~60%, it is in heaps then to stir and evenly mix heap, and ferment 24~36h；Then, turning is carried out,
Suitable quantity of water is added after turning again, and makes the humidity of material be 40~50%, and 48~60h of heap fermentation in heaps；After fermentation again
Secondary turning, and suitable quantity of water is added, so that the humidity of material is 30~45%, heap fermentation 72~84h, as compound fertilizer in heaps again
Material.
9. being carried out in fertilising 1 year the method according to claim 1, wherein the method may further include
The step of top dressing.
10. method according to claim 1 to 9, which is characterized in that the method still further comprises harvesting
The step of Herba Cistanches.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710735006.7A CN109418050B (en) | 2017-08-24 | 2017-08-24 | Method for artificially cultivating cistanche |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710735006.7A CN109418050B (en) | 2017-08-24 | 2017-08-24 | Method for artificially cultivating cistanche |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109418050A true CN109418050A (en) | 2019-03-05 |
| CN109418050B CN109418050B (en) | 2021-09-14 |
Family
ID=65501170
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710735006.7A Active CN109418050B (en) | 2017-08-24 | 2017-08-24 | Method for artificially cultivating cistanche |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109418050B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113079966A (en) * | 2021-04-13 | 2021-07-09 | 中国科学院西北生态环境资源研究院 | Composite porous substrate for cistanche deserticola planting and preparation method and inoculation method thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1647914A (en) * | 2004-12-14 | 2005-08-03 | 新疆博林科技发展有限责任公司 | Inoculating paper for cistanche salsa |
| CN102523886A (en) * | 2012-02-09 | 2012-07-04 | 中国农业大学 | Method for inoculating cistanche tubulosa by infiltrating irrigation technology |
| CN102550264A (en) * | 2012-02-09 | 2012-07-11 | 中国农业大学 | Method for using electric hot bed to inoculate Cistanche tubulosa |
| CN102986420A (en) * | 2012-11-25 | 2013-03-27 | 溧阳市天目湖保健品有限公司 | Cultivating method of eucommia ulmoides |
| CN104472186A (en) * | 2014-12-10 | 2015-04-01 | 新疆林科院造林治沙研究所 | Liquid inoculation method for artificially planting cistanche |
| CN105684655A (en) * | 2015-03-13 | 2016-06-22 | 中国科学院植物研究所 | A method for screening cistanche seeds |
| CN106134686A (en) * | 2015-04-16 | 2016-11-23 | 宁夏农林科学院 | The method that Herba Cistanches is efficiently parasitic |
-
2017
- 2017-08-24 CN CN201710735006.7A patent/CN109418050B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1647914A (en) * | 2004-12-14 | 2005-08-03 | 新疆博林科技发展有限责任公司 | Inoculating paper for cistanche salsa |
| CN102523886A (en) * | 2012-02-09 | 2012-07-04 | 中国农业大学 | Method for inoculating cistanche tubulosa by infiltrating irrigation technology |
| CN102550264A (en) * | 2012-02-09 | 2012-07-11 | 中国农业大学 | Method for using electric hot bed to inoculate Cistanche tubulosa |
| CN102986420A (en) * | 2012-11-25 | 2013-03-27 | 溧阳市天目湖保健品有限公司 | Cultivating method of eucommia ulmoides |
| CN104472186A (en) * | 2014-12-10 | 2015-04-01 | 新疆林科院造林治沙研究所 | Liquid inoculation method for artificially planting cistanche |
| CN105684655A (en) * | 2015-03-13 | 2016-06-22 | 中国科学院植物研究所 | A method for screening cistanche seeds |
| CN106134686A (en) * | 2015-04-16 | 2016-11-23 | 宁夏农林科学院 | The method that Herba Cistanches is efficiently parasitic |
Non-Patent Citations (1)
| Title |
|---|
| 徐良: "《中国名贵药材规范化栽培与产业化开发新技术 中国GAP药材与中药现代化开发指南》", 30 November 2001, 中国协和医科大学出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113079966A (en) * | 2021-04-13 | 2021-07-09 | 中国科学院西北生态环境资源研究院 | Composite porous substrate for cistanche deserticola planting and preparation method and inoculation method thereof |
| CN113079966B (en) * | 2021-04-13 | 2022-04-22 | 中国科学院西北生态环境资源研究院 | Composite porous substrate for cistanche deserticola planting and preparation method and inoculation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109418050B (en) | 2021-09-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102356746B (en) | Technology for cultivating organic fruit and vegetable matrix | |
| CN103922846B (en) | Saline-alkali soil improvement method | |
| CN106416492B (en) | The implantation methods of high-yield rice | |
| CN105284478B (en) | A kind of one-step method breeding method of oil-tea sprout anvil Light media grafting container seedling | |
| CN104541866B (en) | Fertilizing method for organic tomato cultivation | |
| CN104628445A (en) | Special fertilizer and foliage fertilizer for dendrobium nobile and planting method of dendrobium nobile | |
| CN105900723B (en) | A kind of method for culturing seedlings and cultural method of panax japonicus majoris | |
| CN105940924B (en) | The method that Alfalfa southern China is efficiently planted | |
| CN107371785A (en) | A kind of method of artificial cultivation hickory chick comprehensive utilization | |
| CN104737771A (en) | High-sugar dendrobium officinale planting method | |
| CN101715715A (en) | Vertical column type soilless culture method of vegetables | |
| CN105706819A (en) | Simplified and efficient management method for soil in hill sloping field newly-built orchard | |
| CN103265356B (en) | Artificial soil and fertilizer capable of promoting nodulation of plant rhizobia | |
| CN107018789A (en) | A kind of implantation methods of cucumber | |
| CN106922374A (en) | A kind of cloud Radix Angelicae Sinensis method for culturing seedlings of high altitude localities | |
| CN107459421A (en) | A kind of Agricus blazei plantation simultaneously plants bacteria residue while the method for producing organic fertilizer using it | |
| CN108055966A (en) | A kind of implantation methods of selenium-rich oil tea | |
| CN107182490A (en) | A kind of implantation methods of dendrobium candidum | |
| CN107793230A (en) | A kind of method prepared by Kiwi berry biological organic fertilizer | |
| CN107371691A (en) | The cultural method of organic Se-rich, high sugared dendrobium candidum | |
| CN106718485A (en) | A kind of seedling-raising technique of fructus crataegi pinnatifidae | |
| CN109845615A (en) | A kind of rhodiola seedling medium and application thereof | |
| CN104012393B (en) | Vegetable cultivation nutrient bed and vegetable cultivation method | |
| CN108476896A (en) | A kind of hillside fields water retention cultivating method of beautiful millettia root | |
| CN106613217A (en) | Selenium-enriched wheat planting method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20210818 Address after: 730000 No.318 Donggang West Road, Lanzhou City, Gansu Province Applicant after: NORTHWEST INSTITUTE OF ECO-ENVIRONMENT AND RESOURCES, CAS Address before: 730000, Institute of cold and drought, Chinese Academy of Sciences, 320 Donggang West Road, Lanzhou City, Gansu Province Applicant before: Institute of environment and Engineering in cold and dry areas, Chinese Academy of Sciences |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant |