CN109415748A - 用于蛋白生产的脂质代谢的调节 - Google Patents

用于蛋白生产的脂质代谢的调节 Download PDF

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Publication number
CN109415748A
CN109415748A CN201780039286.6A CN201780039286A CN109415748A CN 109415748 A CN109415748 A CN 109415748A CN 201780039286 A CN201780039286 A CN 201780039286A CN 109415748 A CN109415748 A CN 109415748A
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China
Prior art keywords
cell
lipid
product
nucleic acid
lmm
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Pending
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CN201780039286.6A
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English (en)
Chinese (zh)
Inventor
J.巴奇
C.M.斯梅尔斯
T.奈特
R.杨
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Lonza AG
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Lonza AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0097—Oxidoreductases (1.) acting on reduced flavodoxin as donor (1.19)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/19—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19)
    • C12Y114/19001—Stearoyl-CoA 9-desaturase (1.14.19.1), i.e. DELTA9-desaturase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Reproductive Health (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
CN201780039286.6A 2016-05-03 2017-05-03 用于蛋白生产的脂质代谢的调节 Pending CN109415748A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662330973P 2016-05-03 2016-05-03
US62/330,973 2016-05-03
PCT/EP2017/060484 WO2017191165A1 (en) 2016-05-03 2017-05-03 Modulation of lipid metabolism for protein production

Publications (1)

Publication Number Publication Date
CN109415748A true CN109415748A (zh) 2019-03-01

Family

ID=58745198

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CN201780039286.6A Pending CN109415748A (zh) 2016-05-03 2017-05-03 用于蛋白生产的脂质代谢的调节

Country Status (13)

Country Link
US (3) US10655111B2 (https=)
EP (1) EP3452608B1 (https=)
JP (1) JP6959942B2 (https=)
KR (1) KR102536221B1 (https=)
CN (1) CN109415748A (https=)
AU (1) AU2017259010B2 (https=)
CA (1) CA3023038C (https=)
EA (1) EA201892503A1 (https=)
ES (1) ES2938626T3 (https=)
IL (2) IL262646B (https=)
MX (1) MX2018013499A (https=)
SG (1) SG11201809626PA (https=)
WO (1) WO2017191165A1 (https=)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017191165A1 (en) 2016-05-03 2017-11-09 Lonza Ltd Modulation of lipid metabolism for protein production
US20210324366A1 (en) * 2018-07-11 2021-10-21 Cornell University Supported plant plasma membrane lipid bilayer on-a-chip
CN119264211A (zh) 2018-08-27 2025-01-07 瑞泽恩制药公司 拉曼光谱在下游纯化中的应用
US12331320B2 (en) 2018-10-10 2025-06-17 The Research Foundation For The State University Of New York Genome edited cancer cell vaccines
US20230193341A1 (en) * 2018-10-24 2023-06-22 Selexis Sa Expression systems, recombinant cells and uses thereof
EP3942048A1 (en) 2019-03-20 2022-01-26 Regeneron Pharmaceuticals, Inc. Treatment of increased lipid levels with sterol regulatory element binding transcription factor 1 (srebf1) inhibitors
CN110317799B (zh) * 2019-07-18 2026-04-03 江南大学 一种人源岩藻糖转移酶8的原核表达方法及其产品
US20220403398A1 (en) 2019-11-14 2022-12-22 Lonza Ltd Methods of cell selection
CN115029307A (zh) * 2022-07-28 2022-09-09 吉林大学 一种延缓MSCs衰老的方法
WO2026062080A1 (en) 2024-09-17 2026-03-26 Ares Trading S.A. Cell having increased secretion capabilities and method of use

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002095008A2 (en) * 2001-02-02 2002-11-28 Bayer Corporation Methods of modulating surfactant phospholipid production
WO2004111194A2 (en) * 2003-06-11 2004-12-23 Biogen Idec Ma Inc. Method to increase protein production in culture
WO2009062789A1 (en) * 2007-11-13 2009-05-22 Boehringer Ingelheim Pharma Gmbh & Co Kg Improving the secretory capacity in host cells
CN102317459A (zh) * 2008-11-18 2012-01-11 联邦科学技术研究组织 产生ω-3脂肪酸的酶和方法
CN105073995A (zh) * 2013-02-01 2015-11-18 瑟莱克斯公司 增强的转基因表达和加工

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9022545D0 (en) 1990-10-17 1990-11-28 Wellcome Found Culture medium
EP1958962A3 (en) 1997-06-12 2013-05-01 Novartis International Pharmaceutical Ltd. Artificial antibody polypeptides
HK1047109A1 (zh) 1999-10-15 2003-02-07 University Of Massachusetts 作为指定基因干预工具的rna干预轨迹基因
US6326193B1 (en) 1999-11-05 2001-12-04 Cambria Biosciences, Llc Insect control agent
AU2001275474A1 (en) 2000-06-12 2001-12-24 Akkadix Corporation Materials and methods for the control of nematodes
EP1776461A2 (en) 2004-04-16 2007-04-25 DSMIP Assets B.V. Fungal promoters for expressing a gene in a fungal cell
EP1915394B1 (en) * 2005-05-20 2021-01-27 Lonza Biologics plc. High-level expression of recombinant antibody in a mammalian host cell
US20080182249A1 (en) * 2006-09-01 2008-07-31 Fox Brian G Expression systems for mammalian and mycobacterial desaturases
JP6676526B2 (ja) * 2013-08-06 2020-04-08 ロンザ バイオロジクス ピーエルシー 組換えタンパク質の生産のための哺乳動物生産細胞の生成のための手段及び方法
WO2017191165A1 (en) * 2016-05-03 2017-11-09 Lonza Ltd Modulation of lipid metabolism for protein production

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002095008A2 (en) * 2001-02-02 2002-11-28 Bayer Corporation Methods of modulating surfactant phospholipid production
WO2004111194A2 (en) * 2003-06-11 2004-12-23 Biogen Idec Ma Inc. Method to increase protein production in culture
WO2009062789A1 (en) * 2007-11-13 2009-05-22 Boehringer Ingelheim Pharma Gmbh & Co Kg Improving the secretory capacity in host cells
CN102317459A (zh) * 2008-11-18 2012-01-11 联邦科学技术研究组织 产生ω-3脂肪酸的酶和方法
CN105073995A (zh) * 2013-02-01 2015-11-18 瑟莱克斯公司 增强的转基因表达和加工

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HUA等: "Sterol Resistance in CHO Cells Traced to Point Mutation in SREBP Cleavage–Activating Protein", 《CELL》 *
XU等: "Overexpression of SREBP1 (sterol regulatory element binding protein 1) promotes de novo fatty acid synthesis and triacylglycerol accumulation in goat mammary epithelial cells", 《JOURNAL 0F DAIRY SCIENCE》 *
YOKOYAMA等: "SREBP-1, a Basic-Helix-Loop-Helix-Leucine Zipper Protein That Controls Transcription of the Low Density Lipoprotein Receptor Gene", 《CELL》 *

Also Published As

Publication number Publication date
KR20190003651A (ko) 2019-01-09
IL262646A (en) 2018-12-31
WO2017191165A1 (en) 2017-11-09
EP3452608B1 (en) 2022-11-30
IL262646B (en) 2022-08-01
US20230047838A1 (en) 2023-02-16
CA3023038C (en) 2023-03-14
JP6959942B2 (ja) 2021-11-05
IL294241B1 (en) 2023-06-01
SG11201809626PA (en) 2018-11-29
EA201892503A1 (ru) 2019-09-30
US20170321194A1 (en) 2017-11-09
CA3023038A1 (en) 2017-11-09
EP3452608A1 (en) 2019-03-13
US10655111B2 (en) 2020-05-19
US11434474B2 (en) 2022-09-06
MX2018013499A (es) 2019-09-11
IL294241A (en) 2022-08-01
JP2019514399A (ja) 2019-06-06
ES2938626T3 (es) 2023-04-13
AU2017259010B2 (en) 2021-04-22
US20200231944A1 (en) 2020-07-23
BR112018072434A2 (pt) 2019-02-19
AU2017259010A1 (en) 2018-11-22
KR102536221B1 (ko) 2023-05-23
IL294241B2 (en) 2023-10-01

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