CN109406402B - A kind of absorption fluorescence universal cuvette device and measurement method - Google Patents
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Abstract
本发明公开了一种吸收荧光通用比色皿装置及测量方法，旨在提供一种即可以作为荧光光谱测量用，又可以作为吸收光谱测量用的吸收荧光通用比色皿装置。吸收荧光通用比色皿装置包括比色皿组件，比色皿组件包括内玻璃筒与套设在内玻璃筒外侧的外玻璃筒，内玻璃筒的上端开口，内玻璃筒的下端封闭，内玻璃筒与外玻璃筒之间形成环形腔体；固定弧面反射镜，固定弧面反射镜固定设置在环形腔体的前侧；活动弧面反射镜，活动弧面反射镜设置在环形腔体内并能够沿环形腔体的周向滑动。
The invention discloses an absorption fluorescence universal cuvette device and a measurement method, and aims to provide an absorption fluorescence universal cuvette device which can be used for both fluorescence spectrum measurement and absorption spectrum measurement. The universal cuvette device for absorbing fluorescence includes a cuvette assembly, the cuvette assembly includes an inner glass cylinder and an outer glass cylinder sleeved on the outside of the inner glass cylinder, the upper end of the inner glass cylinder is open, the lower end of the inner glass cylinder is closed, and the inner glass cylinder is closed. An annular cavity is formed between the cylinder and the outer glass cylinder; the fixed arc mirror is fixedly arranged on the front side of the annular cavity; the movable arc mirror is arranged in the annular cavity and is It can slide along the circumference of the annular cavity.
Description
技术领域technical field
本发明涉及光谱分析技术领域，具体涉及一种吸收荧光通用比色皿装置及测量方法。The invention relates to the technical field of spectral analysis, in particular to a universal cuvette device for absorption fluorescence and a measurement method.
背景技术Background technique
比色皿作为光谱分析仪器的配件，进行物质的吸收光谱、荧光、磷光、散射光或化学荧光测量时用来盛装调配好的待检测样品溶液（待检测样品溶液包括样品液和参比液），广泛应用于化工，食品，环保，水电、石油和医疗等行业。The cuvette, as an accessory of the spectral analysis instrument, is used to hold the prepared sample solution to be tested (the sample solution to be tested includes the sample solution and the reference solution) when measuring the absorption spectrum, fluorescence, phosphorescence, scattered light or chemical fluorescence of the substance. , widely used in chemical, food, environmental protection, hydropower, petroleum and medical industries.
目前，用于测量吸收光谱的为双通比色皿（比色皿的四个侧面中的两面透光，两面磨砂）；用于测量荧光光谱的为四通比色皿（比色皿的四面透光）；因而在对同一样品的吸收光谱和荧光光谱测量时，需要用两个不同比色皿测量分别进行测量，需要增加溶液倾倒等操作步骤和需要更多的样品溶液不仅操作不便，测量效率低，而且需要更多的样品溶液。At present, the double-pass cuvette (two of the four sides of the cuvette are transparent and the two sides are frosted) for measuring the absorption spectrum; the four-pass cuvette (four sides of the cuvette) is used for measuring the fluorescence spectrum. Therefore, when measuring the absorption spectrum and fluorescence spectrum of the same sample, it is necessary to use two different cuvettes to measure separately, which requires additional operation steps such as solution pouring and more sample solutions, which is not only inconvenient to operate, but also to measure Inefficient and requires more sample solution.
另一方面，待检测样品溶液经一定波长的光照射后会受到激发，被激发的分子或者原子从激发态返回到基态(去激发)时，会发射出波长比入射光长的不同强度的荧光，而所产生的荧光向着各个方向随机发散辐射，因而仅有较少部分荧光信号从四面透光比色皿中传导至检测器中，实际上减小了传导至检测器中的荧光信号。On the other hand, the sample solution to be detected will be excited after being irradiated with light of a certain wavelength, and when the excited molecules or atoms return from the excited state to the ground state (de-excited), they will emit fluorescence of different intensities with wavelengths longer than the incident light. , and the generated fluorescence radiates randomly in all directions, so only a small part of the fluorescence signal is transmitted from the four-sided light-transmitting cuvette to the detector, which actually reduces the fluorescence signal transmitted to the detector.
此外，在测量荧光光谱时，激发光穿透比色皿，还将造成部分激发光损失。In addition, when measuring the fluorescence spectrum, the excitation light penetrates the cuvette, which will also cause part of the excitation light loss.
发明内容SUMMARY OF THE INVENTION
本发明的第一目的是为了提供一种吸收荧光通用比色皿装置及测量方法，其即可以作为荧光光谱测量用，又可以作为吸收光谱测量用。The first object of the present invention is to provide a universal cuvette device and measurement method for absorption fluorescence, which can be used for both fluorescence spectrum measurement and absorption spectrum measurement.
本发明的第二目的是为了提供一种吸收荧光通用比色皿装置及测量方法，其可以增大荧光光谱测量时的荧光信号，提高荧光检测的灵敏度。The second object of the present invention is to provide an absorption fluorescence universal cuvette device and measurement method, which can increase the fluorescence signal during fluorescence spectrum measurement and improve the sensitivity of fluorescence detection.
本发明的技术方案是：The technical scheme of the present invention is:
一种吸收荧光通用比色皿装置，包括：比色皿组件，比色皿组件包括内玻璃筒与套设在内玻璃筒外侧的外玻璃筒，内玻璃筒的上端开口，内玻璃筒的下端封闭，内玻璃筒与外玻璃筒之间形成环形腔体；固定弧面反射镜，固定弧面反射镜固定设置在环形腔体的前侧；活动弧面反射镜，活动弧面反射镜设置在环形腔体内并能够沿环形腔体的周向滑动。A universal cuvette device for absorbing fluorescence, comprising: a cuvette assembly, the cuvette assembly includes an inner glass cylinder and an outer glass cylinder sleeved on the outside of the inner glass cylinder, the upper end of the inner glass cylinder is open, and the lower end of the inner glass cylinder is open. closed, an annular cavity is formed between the inner glass cylinder and the outer glass cylinder; the fixed arc mirror is fixed on the front side of the annular cavity; the movable arc mirror is arranged on the front side of the annular cavity; Inside the annular cavity and capable of sliding along the circumferential direction of the annular cavity.
本方案的吸收荧光通用比色皿装置即可以作为荧光光谱测量用，又可以作为吸收光谱测量用，通用性好；尤其是在对同一样品的吸收光谱和荧光光谱测量时，无需更换样品溶液，可以极大的提高测量效率低。The absorption fluorescence universal cuvette device of this scheme can be used for both fluorescence spectrum measurement and absorption spectrum measurement, and has good versatility; especially when measuring the absorption spectrum and fluorescence spectrum of the same sample, there is no need to replace the sample solution It can greatly improve the measurement efficiency.
作为优选，环形腔体的底部设有环形导轨，所述活动弧面反射镜设置在环形导轨上并能够沿环形导轨滑动。Preferably, the bottom of the annular cavity is provided with an annular guide rail, and the movable arc mirror is arranged on the annular guide rail and can slide along the annular guide rail.
作为优选，固定弧面反射镜的轴线与内玻璃筒的轴线相平行，且固定弧面反射镜的轴线位于内玻璃筒的内侧。Preferably, the axis of the fixed cambered mirror is parallel to the axis of the inner glass cylinder, and the axis of the fixed cambered mirror is located inside the inner glass cylinder.
作为优选，活动弧面反射镜的轴线与内玻璃筒的轴线相平行，且活动弧面反射镜的轴线位于内玻璃筒的内侧。Preferably, the axis of the movable arc reflector is parallel to the axis of the inner glass cylinder, and the axis of the movable arc reflector is located inside the inner glass cylinder.
作为优选，内玻璃筒与外玻璃筒同轴。Preferably, the inner glass cylinder is coaxial with the outer glass cylinder.
一种荧光光谱测量方法，包括用于检测荧光的光谱检测器，荧光光谱测量方法依次包括以下步骤：A fluorescence spectrum measurement method includes a spectrum detector for detecting fluorescence, and the fluorescence spectrum measurement method includes the following steps in sequence:
第一，将调配好的待检测样品溶液倒入内玻璃筒内；First, pour the prepared sample solution to be tested into the inner glass cylinder;
将活动弧面反射镜沿环形腔体滑动至环形腔体的左侧；Slide the movable arc mirror along the annular cavity to the left side of the annular cavity;
将光谱检测器置于比色皿组件的后侧；Place the spectral detector on the rear side of the cuvette assembly;
第二，入射光由比色皿组件的右侧射入比色皿组件内，入射光照射内玻璃筒内的待检测样品溶液，待检测样品溶液受入射光照射后受到激发，发射出荧光；入射光由比色皿组件的右侧射入比色皿组件内并穿过待检测样品溶后，将被活动弧面反射镜反射再次照射内玻璃筒内的待检测样品溶液，实现待检测样品溶液的二次激发，从而有效减小入射光的损失，并增大荧光信号；Second, the incident light is injected into the cuvette assembly from the right side of the cuvette assembly, the incident light irradiates the sample solution to be detected in the inner glass cylinder, and the sample solution to be detected is excited after being irradiated by the incident light, and emits fluorescence; the incident light The right side of the cuvette assembly is injected into the cuvette assembly and passes through the sample to be detected. After being dissolved, the sample solution to be detected in the inner glass cylinder will be reflected by the movable arc mirror and irradiated again. secondary excitation, thereby effectively reducing the loss of incident light and increasing the fluorescence signal;
发射出的荧光向着各个方向随机发散辐射，其中有部分荧光直接由比色皿组件的后侧透射出并传导光谱检测器中，有部分荧光被固定弧面反射镜反射后，由比色皿组件的后侧透射出并传导光谱检测器中，从而增大荧光信号。The emitted fluorescence scatters randomly in all directions, and some of the fluorescence is directly transmitted from the rear side of the cuvette assembly and transmitted to the spectrum detector. The side transmits and conducts into the spectral detector, thereby increasing the fluorescence signal.
一种吸收光谱测量方法，包括光谱检测器，吸收光谱测量方法依次包括以下步骤：An absorption spectrum measurement method, comprising a spectrum detector, and the absorption spectrum measurement method sequentially includes the following steps:
第一，将调配好的待检测样品溶液倒入内玻璃筒内；First, pour the prepared sample solution to be tested into the inner glass cylinder;
将活动弧面反射镜沿环形腔体滑动至环形腔体的后侧；Slide the movable arc mirror along the annular cavity to the rear side of the annular cavity;
将光谱检测器置于比色皿组件的左侧；Place the spectral detector on the left side of the cuvette assembly;
第二，入射光由比色皿组件的右侧射入比色皿组件内，入射光经过待检测样品溶液后由比色皿组件的左侧透射出并传导光谱检测器中。Second, the incident light is injected into the cuvette assembly from the right side of the cuvette assembly, and the incident light is transmitted from the left side of the cuvette assembly after passing through the sample solution to be detected and conducted into the spectrum detector.
本发明的有益效果是：The beneficial effects of the present invention are:
其一，即可以作为荧光光谱测量用，又可以作为吸收光谱测量用，通用性好。First, it can be used for both fluorescence spectrum measurement and absorption spectrum measurement, with good versatility.
其二，可以增大荧光光谱测量时的荧光信号，提高荧光检测的灵敏度。Second, the fluorescence signal during fluorescence spectrum measurement can be increased, and the sensitivity of fluorescence detection can be improved.
附图说明Description of drawings
图1是本发明的实施例1的吸收荧光通用比色皿装置的一种结构示意图。FIG. 1 is a schematic structural diagram of a general-purpose cuvette device for absorbing fluorescence according to
图2是图1的俯视图。FIG. 2 is a plan view of FIG. 1 .
图3是本发明的实施例1的固定弧面反射镜的一种结构示意图。FIG. 3 is a schematic structural diagram of the fixed arc surface mirror according to
图4是本发明的实施例2的一种荧光光谱测量方法中吸收荧光通用比色皿装置与光谱检测器的一种结构示意图。4 is a schematic structural diagram of an absorption fluorescence universal cuvette device and a spectrum detector in a fluorescence spectrum measurement method according to
图5是本发明的实施例3的一种吸收光谱测量方法中吸收荧光通用比色皿装置与光谱检测器的一种结构示意图。5 is a schematic structural diagram of an absorption fluorescence universal cuvette device and a spectrum detector in an absorption spectrum measurement method according to
图中：In the picture:
比色皿组件1，内玻璃筒1.1，外玻璃筒1.2，环形腔体1.3；
固定弧面反射镜2；Fixed
活动弧面反射镜3；Active
光谱检测器4。
具体实施方式Detailed ways
为使本发明技术方案实施例目的、技术方案和优点更加清楚，下面结合附图对本发明实施例的技术方案进行清楚地解释和说明，但下述实施例仅为本发明的优选实施例，而不是全部实施例。基于实施方式中的实施例，本领域技术人员在没有做出创造性劳动的前提下所获得其他实施例，都属于本发明的保护范围。In order to make the purposes, technical solutions and advantages of the embodiments of the technical solutions of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly explained and described below with reference to the accompanying drawings, but the following embodiments are only preferred embodiments of the present invention, and Not all examples. Based on the examples in the implementation manner, other examples obtained by those skilled in the art without creative work shall fall within the protection scope of the present invention.
下面详细描述本发明的实施例，所述实施例的示例在附图中示出，其中自始至终相同或类似的标号表示相同或类似的元件或具有相同或类似功能的元件。下面通过参考附图描述的实施例是示例性的，仅用于解释本方案，而不能解释为对本发明方案的限制。The following describes in detail the embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein the same or similar reference numerals refer to the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary, and are only used to explain the present solution, but cannot be construed as a limitation on the solution of the present invention.
参照下面的描述和附图，将清楚本发明的实施例的这些和其他方面。在这些描述和附图中，具体公开了本发明的实施例中的一些特定实施方式来表示实施本发明的实施例的原理的一些方式，但是应当理解，本发明的实施例的范围不受此限制。相反，本发明的实施例包括落入所附加权利要求书的精神和内涵范围内的所有变化、修改和等同物。These and other aspects of embodiments of the present invention will become apparent with reference to the following description and accompanying drawings. In these descriptions and drawings, some specific implementations of the embodiments of the invention are disclosed in detail to represent some ways of implementing the principles of the embodiments of the invention, but it should be understood that the scope of the embodiments of the invention is not limited thereby limit. On the contrary, embodiments of the present invention include all changes, modifications and equivalents falling within the spirit and scope of the appended claims.
在本发明的描述中，需要理解的是，术语“厚度”、“上”、“下”、“水平”、“顶”、“底”、“内”、“外”、“周向”等指示的方位或位置关系为基于附图所示的方位或位置关系，仅是为了便于描述本发明和简化描述，而不是指示或暗示所指的装置或元件必须具有特定的方位、以特定的方位构造和操作，因此不能理解为对本发明的限制。在本发明的描述中，“多个”的含义是至少两个，例如两个，三个等，除非另有明确具体的限定，“若干”的含义是表示一个或者多个。In the description of the present invention, it should be understood that the terms "thickness", "upper", "lower", "horizontal", "top", "bottom", "inner", "outer", "circumferential", etc. The indicated orientation or positional relationship is based on the orientation or positional relationship shown in the accompanying drawings, which is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the indicated device or element must have a specific orientation or a specific orientation. construction and operation, and therefore should not be construed as limiting the invention. In the description of the present invention, "plurality" means at least two, such as two, three, etc., unless otherwise expressly and specifically defined, the meaning of "several" means one or more.
在本发明中，除非另有明确的规定和限定，术语“安装”、“相连”、“连接”、“固定”等术语应做广义理解，例如，可以是固定连接，也可以是可拆卸连接，或成一体:可以是机械连接，也可以是电连接或彼此可通讯；可以是直接相连，也可以通过中间媒介间接相连，可以是两个元件内部的连通或两个元件的相互作用关系，除非另有明确的限定。对于本领域的普通技术人员而言，可以根据具体情况理解上述术语在本发明中的具体含义。In the present invention, unless otherwise expressly specified and limited, terms such as "installation", "connection", "connection", "fixation" and other terms should be understood in a broad sense, for example, it may be a fixed connection or a detachable connection , or integrated: it can be a mechanical connection or an electrical connection or can communicate with each other; it can be directly connected or indirectly connected through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between the two elements, unless otherwise expressly qualified. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.
具体实施例一：如图1 、图2、图3所示， 一种吸收荧光通用比色皿装置，包括比色皿组件1、固定弧面反射镜2及活动弧面反射镜3。Specific embodiment 1: As shown in FIG. 1 , FIG. 2 , and FIG. 3 , a universal cuvette device for absorbing fluorescence includes a
比色皿组件包括内玻璃筒1.1与套设在内玻璃筒外侧的外玻璃筒1.2。内玻璃筒与外玻璃筒同轴。内玻璃筒的上端开口，内玻璃筒的下端封闭。内玻璃筒与外玻璃筒之间形成环形腔体1.3。环形腔体的底部设有环形导轨（图中未示出）。The cuvette assembly includes an inner glass cylinder 1.1 and an outer glass cylinder 1.2 sleeved outside the inner glass cylinder. The inner glass cylinder is coaxial with the outer glass cylinder. The upper end of the inner glass cylinder is open, and the lower end of the inner glass cylinder is closed. An annular cavity 1.3 is formed between the inner glass cylinder and the outer glass cylinder. The bottom of the annular cavity is provided with an annular guide rail (not shown in the figure).
固定弧面反射镜2固定设置在环形腔体的前侧。固定弧面反射镜的轴线与内玻璃筒的轴线相平行，且固定弧面反射镜的轴线位于内玻璃筒的内侧。固定弧面反射镜的镜面朝向内玻璃筒。The fixed
活动弧面反射镜3设置在环形腔体内并能够沿环形腔体的周向滑动，具体说是，活动弧面反射镜设置在环形导轨上并能够沿环形导轨滑动。活动弧面反射镜的轴线与内玻璃筒的轴线相平行，且活动弧面反射镜的轴线位于内玻璃筒的内侧。活动弧面反射镜的镜面朝向内玻璃筒。The
具体实施例二，如图4所示，一种荧光光谱测量方法，包括吸收荧光通用比色皿装置与用于检测荧光的光谱检测器4，其中吸收荧光通用比色皿装置的具体结构参照实施例1。
荧光光谱测量方法依次包括以下步骤：The fluorescence spectrum measurement method sequentially includes the following steps:
第一，将调配好的待检测样品溶液倒入内玻璃筒内；First, pour the prepared sample solution to be tested into the inner glass cylinder;
将活动弧面反射镜3沿环形腔体滑动至环形腔体的左侧；Slide the
将光谱检测器置于比色皿组件的后侧；Place the spectral detector on the rear side of the cuvette assembly;
第二，入射光由比色皿组件的右侧射入比色皿组件内，入射光照射内玻璃筒内的待检测样品溶液，待检测样品溶液受入射光照射后受到激发，发射出荧光；入射光由比色皿组件的右侧射入比色皿组件内并穿过待检测样品溶后，将被活动弧面反射镜反射再次照射内玻璃筒内的待检测样品溶液，实现待检测样品溶液的二次激发，从而有效减小入射光的损失，并增大荧光信号，提高荧光检测的灵敏度；Second, the incident light is injected into the cuvette assembly from the right side of the cuvette assembly, the incident light irradiates the sample solution to be detected in the inner glass cylinder, and the sample solution to be detected is excited after being irradiated by the incident light and emits fluorescence; the incident light The right side of the cuvette assembly is injected into the cuvette assembly and passes through the sample to be detected. After being dissolved, it will be reflected by the movable arc mirror to illuminate the sample solution to be detected in the inner glass cylinder again, so as to realize the two-phase flow of the sample solution to be detected. secondary excitation, thereby effectively reducing the loss of incident light, increasing the fluorescence signal, and improving the sensitivity of fluorescence detection;
发射出的荧光向着各个方向随机发散辐射，其中有部分荧光直接由比色皿组件的后侧透射出并传导光谱检测器中，还有部分荧光被固定弧面反射镜反射后，由比色皿组件的后侧透射出并传导光谱检测器中，从而增大荧光信号，提高荧光检测的灵敏度。The emitted fluorescence scatters randomly in all directions, and some of the fluorescence is directly transmitted from the back side of the cuvette assembly and conducted into the spectral detector, and some of the fluorescence is reflected by the fixed curved mirror and then transmitted by the cuvette assembly. The rear side transmits and conducts into the spectral detector, thereby increasing the fluorescence signal and improving the sensitivity of fluorescence detection.
具体实施例三，如图5所示，一种吸收光谱测量方法，包括吸收荧光通用比色皿装置与光谱检测器，其中吸收荧光通用比色皿装置的具体结构参照实施例1。
吸收光谱测量方法依次包括以下步骤：The absorption spectrum measurement method sequentially includes the following steps:
第一，将调配好的待检测样品溶液倒入内玻璃筒内；First, pour the prepared sample solution to be tested into the inner glass cylinder;
将活动弧面反射镜沿环形腔体滑动至环形腔体的后侧；Slide the movable arc mirror along the annular cavity to the rear side of the annular cavity;
将光谱检测器置于比色皿组件的左侧；Place the spectral detector on the left side of the cuvette assembly;
第二，入射光由比色皿组件的右侧射入比色皿组件内，入射光经过待检测样品溶液后由比色皿组件的左侧透射出并传导光谱检测器中，其中，固定弧面反射镜与活动弧面反射镜位于环形腔体的前后两侧，用于阻挡外界光线由比色皿组件的前后两侧透射入，影响吸收光谱测量的准确性。Second, the incident light is injected into the cuvette assembly from the right side of the cuvette assembly, and the incident light is transmitted from the left side of the cuvette assembly after passing through the sample solution to be detected and transmitted to the spectral detector, wherein the fixed arc surface reflects The mirror and the movable arc reflector are located on the front and rear sides of the annular cavity, which are used to block the transmission of external light from the front and rear sides of the cuvette assembly, which affects the accuracy of the absorption spectrum measurement.
本案中吸收荧光通用比色皿装置即可以作为荧光光谱测量用，又可以作为吸收光谱测量用，通用性好；尤其是在对同一样品的吸收光谱和荧光光谱测量时，无需更换样品溶液，可以极大的提高测量效率低。The absorption and fluorescence universal cuvette device in this case can be used for both fluorescence spectrum measurement and absorption spectrum measurement, with good versatility; Greatly improve the measurement efficiency.
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