CN109406402B - Universal cuvette device for absorbing fluorescence and measurement method - Google Patents
Universal cuvette device for absorbing fluorescence and measurement method Download PDFInfo
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- CN109406402B CN109406402B CN201811033242.5A CN201811033242A CN109406402B CN 109406402 B CN109406402 B CN 109406402B CN 201811033242 A CN201811033242 A CN 201811033242A CN 109406402 B CN109406402 B CN 109406402B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N21/0303—Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6482—Sample cells, cuvettes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6495—Miscellaneous methods
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Abstract
The invention discloses a universal cuvette device for absorption fluorescence and a measurement method thereof, and aims to provide a universal cuvette device for absorption fluorescence, which can be used for fluorescence spectrum measurement and absorption spectrum measurement. The universal cuvette device for absorbing fluorescence comprises a cuvette assembly, wherein the cuvette assembly comprises an inner glass cylinder and an outer glass cylinder sleeved outside the inner glass cylinder, the upper end of the inner glass cylinder is opened, the lower end of the inner glass cylinder is closed, and an annular cavity is formed between the inner glass cylinder and the outer glass cylinder; the fixed cambered reflector is fixedly arranged on the front side of the annular cavity; the movable cambered reflector is arranged in the annular cavity and can slide along the circumferential direction of the annular cavity.
Description
Technical Field
The invention relates to the technical field of spectral analysis, in particular to a universal cuvette device for absorbing fluorescence and a measurement method.
Background
The cuvette is used as an accessory of a spectral analysis instrument, is used for containing prepared sample solution to be detected (the sample solution to be detected comprises sample solution and reference solution) when measuring absorption spectrum, fluorescence, phosphorescence, scattered light or chemical fluorescence of a substance, and is widely applied to industries such as chemical industry, food, environmental protection, water and electricity, petroleum, medical treatment and the like.
Currently, two-way cuvettes (cuvettes with two of their four sides transparent and two frosted) are used for measuring absorption spectra; used for measuring the fluorescence spectrum is a four-way cuvette (the cuvette is transparent on four sides); therefore, when the absorption spectrum and the fluorescence spectrum of the same sample are measured, two different cuvettes are needed to be used for measurement respectively, operation steps such as solution dumping are needed to be added, and more sample solutions are needed, so that the operation is inconvenient, the measurement efficiency is low, and more sample solutions are needed.
On the other hand, the sample solution to be detected is excited after being irradiated with light of a certain wavelength, and when the excited molecules or atoms return from the excited state to the ground state (deexcitation), fluorescence of different intensities having a longer wavelength than the incident light is emitted, and the generated fluorescence is randomly radiated in all directions, so that only a small part of the fluorescence signal is transmitted from the four-sided light-transmitting cuvette to the detector, and the fluorescence signal transmitted to the detector is actually reduced.
In addition, when measuring the fluorescence spectrum, the excitation light penetrates the cuvette, which also causes a partial loss of excitation light.
Disclosure of Invention
The first object of the present invention is to provide a universal cuvette device for absorption fluorescence and a measurement method thereof, which can be used for both fluorescence spectrum measurement and absorption spectrum measurement.
The second object of the present invention is to provide a universal cuvette device for absorbing fluorescence and a measurement method thereof, which can increase the fluorescence signal during fluorescence spectrum measurement and improve the sensitivity of fluorescence detection.
The technical scheme of the invention is as follows:
an absorbing fluorescent universal cuvette device, comprising: the cuvette assembly comprises an inner glass cylinder and an outer glass cylinder sleeved outside the inner glass cylinder, the upper end of the inner glass cylinder is open, the lower end of the inner glass cylinder is closed, and an annular cavity is formed between the inner glass cylinder and the outer glass cylinder; the fixed cambered reflector is fixedly arranged on the front side of the annular cavity; the movable cambered reflector is arranged in the annular cavity and can slide along the circumferential direction of the annular cavity.
The universal cuvette device for absorbing fluorescence can be used for measuring fluorescence spectra and absorption spectra, and has good universality; especially, when the absorption spectrum and the fluorescence spectrum of the same sample are measured, the sample solution does not need to be replaced, and the measurement efficiency can be greatly improved.
Preferably, the bottom of the annular cavity is provided with an annular guide rail, and the movable cambered reflector is arranged on the annular guide rail and can slide along the annular guide rail.
Preferably, the axis of the fixed cambered reflector is parallel to the axis of the inner glass cylinder, and the axis of the fixed cambered reflector is positioned on the inner side of the inner glass cylinder.
Preferably, the axis of the movable cambered reflector is parallel to the axis of the inner glass cylinder, and the axis of the movable cambered reflector is positioned on the inner side of the inner glass cylinder.
Preferably, the inner glass cylinder is coaxial with the outer glass cylinder.
A fluorescence spectrum measuring method including a spectrum detector for detecting fluorescence, the fluorescence spectrum measuring method comprising the steps of, in order:
firstly, pouring a prepared sample solution to be detected into an inner glass cylinder;
sliding the movable cambered reflector to the left side of the annular cavity along the annular cavity;
placing a spectral detector on a rear side of the cuvette assembly;
secondly, incident light is emitted into the cuvette assembly from the right side of the cuvette assembly, the incident light irradiates a sample solution to be detected in the inner glass cylinder, and the sample solution to be detected is excited after being irradiated by the incident light and emits fluorescence; incident light is emitted into the cuvette assembly from the right side of the cuvette assembly and penetrates through a sample to be detected to be dissolved, and then is reflected by the movable cambered reflector to irradiate the sample solution to be detected in the inner glass cylinder again, so that secondary excitation of the sample solution to be detected is realized, the loss of the incident light is effectively reduced, and a fluorescence signal is increased;
the emitted fluorescence is scattered and radiated randomly in all directions, wherein part of the fluorescence is directly transmitted out of the rear side of the cuvette assembly and is conducted in the spectral detector, and part of the fluorescence is reflected by the fixed cambered reflector and then is transmitted out of the rear side of the cuvette assembly and is conducted in the spectral detector, so that the fluorescence signal is increased.
An absorption spectroscopy measurement method comprising a spectral detector, the absorption spectroscopy measurement method comprising the following steps in sequence:
firstly, pouring a prepared sample solution to be detected into an inner glass cylinder;
sliding the movable cambered reflector to the rear side of the annular cavity along the annular cavity;
placing a spectral detector on the left side of the cuvette assembly;
secondly, incident light is emitted into the cuvette assembly from the right side of the cuvette assembly, and the incident light is transmitted out from the left side of the cuvette assembly after passing through a sample solution to be detected and is transmitted into the spectral detector.
The invention has the beneficial effects that:
the device can be used for measuring fluorescence spectra and absorption spectra, and has good universality.
Secondly, the fluorescence signal during fluorescence spectrum measurement can be increased, and the sensitivity of fluorescence detection is improved.
Drawings
FIG. 1 is a schematic diagram showing a structure of an absorptive-fluorescent universal cuvette assembly according to example 1 of the present invention.
Fig. 2 is a top view of fig. 1.
Fig. 3 is a schematic structural view of a fixed-arc reflector according to embodiment 1 of the present invention.
FIG. 4 is a schematic diagram showing a configuration of the universal cuvette assembly for absorbing fluorescence and the spectral detector in the fluorescence spectrum measuring method according to embodiment 2 of the present invention.
FIG. 5 is a schematic diagram showing a configuration of an absorption fluorescence universal cuvette assembly and a spectrum detector in an absorption spectrum measurement method according to embodiment 3 of the present invention.
In the figure:
the cuvette assembly 1, the inner glass cylinder 1.1, the outer glass cylinder 1.2 and the annular cavity 1.3;
fixing the cambered surface reflector 2;
a movable arc reflector 3;
a spectral detector 4.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention are clearly explained and illustrated below with reference to the accompanying drawings, but the following embodiments are only preferred embodiments of the present invention, and not all embodiments. Based on the embodiments in the implementation, other embodiments obtained by those skilled in the art without any creative effort belong to the protection scope of the present invention.
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below by referring to the drawings are exemplary only for explaining the present scheme, and are not construed as limiting the scheme of the present invention.
These and other aspects of embodiments of the invention will be apparent with reference to the following description and attached drawings. In the description and drawings, particular embodiments of the invention have been disclosed in detail as being indicative of some of the ways in which the principles of the embodiments of the invention may be practiced, but it is understood that the scope of the embodiments of the invention is not limited thereby. On the contrary, the embodiments of the invention include all changes, modifications and equivalents coming within the spirit and terms of the claims appended hereto.
In the description of the present invention, it is to be understood that the terms "thickness", "upper", "lower", "horizontal", "top", "bottom", "inner", "outer", "circumferential", and the like, indicate orientations and positional relationships based on those shown in the drawings, and are used merely for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., and "several" means one or more unless specifically limited otherwise.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "fixed," and the like are to be construed broadly and can include, for example, fixed connections, removable connections, or integral connections, either mechanical or electrical, or communicating with each other; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
The first embodiment is as follows: as shown in fig. 1, 2 and 3, a universal cuvette device for absorbing fluorescence includes a cuvette assembly 1, a fixed arc reflector 2 and a movable arc reflector 3.
The cuvette assembly comprises an inner glass cylinder 1.1 and an outer glass cylinder 1.2 sleeved outside the inner glass cylinder. The inner glass cylinder is coaxial with the outer glass cylinder. The upper end of the inner glass cylinder is opened, and the lower end of the inner glass cylinder is closed. An annular cavity 1.3 is formed between the inner glass cylinder and the outer glass cylinder. The bottom of the annular cavity is provided with an annular guide rail (not shown in the figure).
The fixed cambered reflector 2 is fixedly arranged on the front side of the annular cavity. The axis of the fixed cambered reflector is parallel to the axis of the inner glass cylinder, and the axis of the fixed cambered reflector is positioned on the inner side of the inner glass cylinder. The mirror surface of the fixed cambered surface reflector faces the inner glass cylinder.
The movable cambered reflector 3 is arranged in the annular cavity and can slide along the circumferential direction of the annular cavity, and specifically, the movable cambered reflector is arranged on the annular guide rail and can slide along the annular guide rail. The axis of the movable cambered reflector is parallel to the axis of the inner glass cylinder, and the axis of the movable cambered reflector is positioned on the inner side of the inner glass cylinder. The mirror surface of the movable cambered surface reflector faces the inner glass cylinder.
Second embodiment, as shown in fig. 4, a fluorescence spectrum measuring method includes an absorption fluorescence universal cuvette device and a spectrum detector 4 for detecting fluorescence, wherein the specific structure of the absorption fluorescence universal cuvette device is as in example 1.
The fluorescence spectrum measurement method sequentially comprises the following steps:
firstly, pouring a prepared sample solution to be detected into an inner glass cylinder;
sliding the movable cambered reflector 3 to the left side of the annular cavity along the annular cavity;
placing a spectral detector on a rear side of the cuvette assembly;
secondly, incident light is emitted into the cuvette assembly from the right side of the cuvette assembly, the incident light irradiates a sample solution to be detected in the inner glass cylinder, and the sample solution to be detected is excited after being irradiated by the incident light and emits fluorescence; incident light is emitted into the cuvette assembly from the right side of the cuvette assembly and penetrates through a sample to be detected to be dissolved, and then is reflected by the movable cambered reflector to irradiate the sample solution to be detected in the inner glass cylinder again, so that secondary excitation of the sample solution to be detected is realized, the loss of the incident light is effectively reduced, a fluorescence signal is increased, and the sensitivity of fluorescence detection is improved;
the emitted fluorescence is scattered and radiated randomly in all directions, wherein part of the fluorescence is directly transmitted out of the rear side of the cuvette component and is conducted into the spectrum detector, and part of the fluorescence is reflected by the fixed cambered reflector and is transmitted out of the rear side of the cuvette component and is conducted into the spectrum detector, so that the fluorescence signal is increased, and the sensitivity of fluorescence detection is improved.
Third embodiment, as shown in fig. 5, an absorption spectrum measuring method includes an absorption fluorescence universal cuvette device and a spectrum detector, wherein the specific structure of the absorption fluorescence universal cuvette device refers to embodiment 1.
The absorption spectrum measuring method sequentially comprises the following steps:
firstly, pouring a prepared sample solution to be detected into an inner glass cylinder;
sliding the movable cambered reflector to the rear side of the annular cavity along the annular cavity;
placing a spectral detector on the left side of the cuvette assembly;
and secondly, the incident light is emitted into the cuvette assembly from the right side of the cuvette assembly, the incident light is transmitted out from the left side of the cuvette assembly after passing through a sample solution to be detected and is conducted in the spectral detector, wherein the fixed cambered surface reflector and the movable cambered surface reflector are positioned on the front side and the rear side of the annular cavity and are used for blocking external light from being transmitted into the front side and the rear side of the cuvette assembly to influence the accuracy of absorption spectrum measurement.
The universal cuvette device for absorbing fluorescence in the scheme can be used for measuring fluorescence spectrum and absorption spectrum, and has good universality; especially, when the absorption spectrum and the fluorescence spectrum of the same sample are measured, the sample solution does not need to be replaced, and the measurement efficiency can be greatly improved.
Claims (7)
1. A universal cuvette device for absorbing fluorescence, comprising:
the cuvette assembly comprises an inner glass cylinder and an outer glass cylinder sleeved outside the inner glass cylinder, the upper end of the inner glass cylinder is open, the lower end of the inner glass cylinder is closed, and an annular cavity is formed between the inner glass cylinder and the outer glass cylinder;
the fixed cambered reflector is fixedly arranged on the front side of the annular cavity;
the movable cambered reflector is arranged in the annular cavity and can slide along the circumferential direction of the annular cavity.
2. The universal cuvette device for absorbing fluorescence according to claim 1, wherein the bottom of the annular cavity is provided with an annular guide rail, and the movable arc reflector is arranged on the annular guide rail and can slide along the annular guide rail.
3. The universal cuvette device for absorbing fluorescence according to claim 1, wherein the axis of the fixed curved reflector is parallel to the axis of the inner glass cylinder, and the axis of the fixed curved reflector is located inside the inner glass cylinder.
4. The universal cuvette device for absorbing fluorescence according to claim 1, wherein the axis of the movable curved reflector is parallel to the axis of the inner glass cylinder, and the axis of the movable curved reflector is located inside the inner glass cylinder.
5. The universal cuvette device according to claim 1, wherein the inner glass cylinder is coaxial with the outer glass cylinder.
6. A fluorescence spectrum measuring method using the universal cuvette assembly for absorbing fluorescence according to claim 1, comprising a spectrum detector for detecting fluorescence, the fluorescence spectrum measuring method comprising the steps of:
firstly, pouring a prepared sample solution to be detected into an inner glass cylinder;
sliding the movable cambered reflector to the left side of the annular cavity along the annular cavity;
placing a spectral detector on a rear side of the cuvette assembly;
secondly, incident light is emitted into the cuvette assembly from the right side of the cuvette assembly, the incident light irradiates a sample solution to be detected in the inner glass cylinder, and the sample solution to be detected is excited after being irradiated by the incident light and emits fluorescence; incident light is emitted into the cuvette assembly from the right side of the cuvette assembly and penetrates through a sample to be detected to be dissolved, and then is reflected by the movable cambered reflector to irradiate the sample solution to be detected in the inner glass cylinder again, so that secondary excitation of the sample solution to be detected is realized, the loss of the incident light is effectively reduced, and a fluorescence signal is increased;
the emitted fluorescence is scattered and radiated randomly in all directions, wherein part of the fluorescence is directly transmitted out of the rear side of the cuvette assembly and is conducted in the spectral detector, and part of the fluorescence is reflected by the fixed cambered reflector and then is transmitted out of the rear side of the cuvette assembly and is conducted in the spectral detector, so that the fluorescence signal is increased.
7. An absorption spectrum measurement method using the universal cuvette assembly for absorption and fluorescence according to claim 1, comprising a spectrum detector, the absorption spectrum measurement method comprising the following steps in sequence:
firstly, pouring a prepared sample solution to be detected into an inner glass cylinder;
sliding the movable cambered reflector to the rear side of the annular cavity along the annular cavity;
placing a spectral detector on the left side of the cuvette assembly;
secondly, incident light is emitted into the cuvette assembly from the right side of the cuvette assembly, and the incident light is transmitted out from the left side of the cuvette assembly after passing through a sample solution to be detected and is transmitted into the spectral detector.
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| CN201811033242.5A CN109406402B (en) | 2018-09-05 | 2018-09-05 | Universal cuvette device for absorbing fluorescence and measurement method |
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| CN201811033242.5A CN109406402B (en) | 2018-09-05 | 2018-09-05 | Universal cuvette device for absorbing fluorescence and measurement method |
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| CN109406402B true CN109406402B (en) | 2020-12-11 |
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| CN112665826A (en) * | 2019-10-15 | 2021-04-16 | 成都辰显光电有限公司 | Integrating sphere detector |
| CN113029964B (en) * | 2021-05-25 | 2021-08-27 | 上海奥普生物医药股份有限公司 | Sample reaction container, diaphragm and optical detection device |
Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000019114A (en) * | 1998-07-03 | 2000-01-21 | Hitachi Ltd | Method and apparatus for detecting faint fluorescence |
| CN1252586A (en) * | 1998-10-26 | 2000-05-10 | 明碁电脑股份有限公司 | Refrection/peneting type scanner |
| CN2588397Y (en) * | 2002-12-27 | 2003-11-26 | 姜强 | Single light source changeable reflection/transmission integrated film direct light-sensing scanner |
| CN101706434A (en) * | 2009-09-04 | 2010-05-12 | 中国环境科学研究院 | Fluorophototmeter capable of detecting light absorption or light transmission |
| CN105115954A (en) * | 2015-09-11 | 2015-12-02 | 深圳世绘林科技有限公司 | Fluorescence spectrophotometer based on optical integrating sphere |
| CN205229031U (en) * | 2015-12-24 | 2016-05-11 | 北京环宇宏业科技开发有限公司 | Novel colorimetry instrument with detecting pond structure |
| CN205656118U (en) * | 2016-04-29 | 2016-10-19 | 浙江医药高等专科学校 | Double reflection of light colorimetric pool of fluorescence |
| CN205719922U (en) * | 2016-05-25 | 2016-11-23 | 广西科技大学 | For measuring cuvette and the optical system thereof of scattering spectrum |
| CN205785074U (en) * | 2016-05-24 | 2016-12-07 | 西安工程大学 | The laser facula width of measuring device measured based on Michelson interference |
| CN106225715A (en) * | 2016-08-02 | 2016-12-14 | 中国科学院长春光学精密机械与物理研究所 | A kind of pentaprism scanning detection method for non-spherical reflector |
| CN106323873A (en) * | 2016-10-11 | 2017-01-11 | 桂林电子科技大学 | Transmitting/reflecting-combined fluorescent multiplication cuvette |
| CN206772438U (en) * | 2017-04-14 | 2017-12-19 | 中山大学 | Absorption and fluorescence spectrum detecting device based on mobile intelligent terminal |
| CN107748142A (en) * | 2017-09-30 | 2018-03-02 | 南京南瑞集团公司 | A kind of dual-beam based on miniature beam-splitting optical system becomes light path sample spectra analytical equipment |
| CN107807236A (en) * | 2017-10-31 | 2018-03-16 | 南京先进激光技术研究院 | One-color fluorescence detection means |
Family Cites Families (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2289881A2 (en) * | 1974-11-04 | 1976-05-28 | Setim | LONG PRODUCT MEASUREMENT DEVICE |
| US4115011A (en) * | 1976-10-07 | 1978-09-19 | Coulter Electronics, Inc. | Cuvette configured for photoanalysis use |
| GB2131195A (en) * | 1982-10-26 | 1984-06-13 | Delphi Ind Limited | A fitter holder for a haemoglobin density comparator |
| US4616139A (en) * | 1983-05-31 | 1986-10-07 | Hauni-Werke Korber & Co. Kg. | Apparatus for optical scanning of the exterior of a moving cigarette rod or the like |
| JP2794576B2 (en) * | 1988-08-29 | 1998-09-10 | 株式会社ロゼフテクノロジー | Pinhole detection device for cylindrical body |
| US5104218A (en) * | 1989-11-09 | 1992-04-14 | General Atomics | Micropipette adaptor for spectrofluorimeters |
| DE4002436A1 (en) * | 1990-01-27 | 1991-08-01 | Man Technologie Gmbh | GAS CUVETTE FOR MATERIAL ANALYSIS |
| DE4002631A1 (en) * | 1990-01-30 | 1991-08-01 | Rosemount Gmbh & Co | Gap absorption spectrum analysis cuvette - has rhodium coating alone or on backing gold and diffusion barrier layers |
| WO1993003350A1 (en) * | 1991-08-01 | 1993-02-18 | Birkle Sensor Gmbh + Co. | Optical components for optically scanning the surface of an object whose surface is capable of reflecting, scattering or refracting light |
| JPH0534270A (en) * | 1991-08-02 | 1993-02-09 | Olympus Optical Co Ltd | Cuvette for biochemical analysis |
| JPH05240774A (en) * | 1992-03-02 | 1993-09-17 | Hitachi Ltd | Optical cell and optical detecting device and sample separating/detecting device using them |
| US6628394B2 (en) * | 2000-02-25 | 2003-09-30 | Hach Company | Optical gasket system |
| DE10058469C1 (en) * | 2000-11-24 | 2002-05-02 | Draeger Safety Ag & Co Kgaa | Robust, compact optical gas sensor, comprises reflective annular chamber promoting long, multiply-reflected circumferential beam path |
| DE10124055A1 (en) * | 2001-05-16 | 2002-11-21 | Fisher Rosemount Mfg Gmbh & Co | Apparatus, used for optically investigating gases, has hollow mirrors with symmetrical mirror surfaces in measuring chamber, to symmetrically rotate about its central axis |
| CN102866113A (en) * | 2012-09-26 | 2013-01-09 | 无锡耐思生物科技有限公司 | Standard 10MM cuvette structure |
| CN103439261B (en) * | 2013-09-25 | 2015-05-13 | 天津亿利科能源科技发展股份有限公司 | Parabolic-mirror-based open type optical long-range pool |
| TWM502505U (en) * | 2014-09-02 | 2015-06-11 | Ezinstrument Technology Co Ltd | A cuvette's holder |
| CN104406913A (en) * | 2014-12-14 | 2015-03-11 | 苏州卫水环保科技有限公司 | Large-optical-path detection device |
| DE102015011810B4 (en) * | 2015-09-17 | 2018-03-15 | Dräger Safety AG & Co. KGaA | Sample gas cuvette for an optical gas sensor and optical gas sensor |
| CN205374302U (en) * | 2016-01-13 | 2016-07-06 | 中国石油大学(华东) | Transparent liquid refractometry appearance |
| CN106018344A (en) * | 2016-05-16 | 2016-10-12 | 中国电子科技集团公司第四十研究所 | Miniaturized Fourier spectrometer based on linear reciprocation lead screw |
| CN206258367U (en) * | 2016-10-11 | 2017-06-16 | 桂林电子科技大学 | A kind of transflection modular fluorometer multiplication cuvette |
| CN206146824U (en) * | 2016-11-10 | 2017-05-03 | 湖北大海化工实业有限公司 | Paint lacquer look detects uses colorimetric device |
| CN206638592U (en) * | 2017-03-21 | 2017-11-14 | 杨宇 | A kind of colorimetric device for quick detection kit |
| CN206920317U (en) * | 2017-04-01 | 2018-01-23 | 苏州科技大学 | A kind of cuvette for optical measurement |
| CN107064065A (en) * | 2017-04-10 | 2017-08-18 | 清华大学 | Measuring device for liquid refractive index and measuring method |
| CN107271366B (en) * | 2017-06-28 | 2020-01-17 | 中南民族大学 | Colorimetric bottle/tube for detecting nitrophenol |
| CN207263218U (en) * | 2017-09-06 | 2018-04-20 | 安徽工业大学 | A kind of device of shifting method measurement transparency liquid refractive index and temperature |
| CN107748152B (en) * | 2017-10-12 | 2019-12-13 | 齐鲁工业大学 | Microorganism detection apparatus and method |
| CN108061707A (en) * | 2017-12-08 | 2018-05-22 | 湖南文理学院 | A kind of integration sphere light source system and test method |
-
2018
- 2018-09-05 CN CN201811033242.5A patent/CN109406402B/en active Active
Patent Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000019114A (en) * | 1998-07-03 | 2000-01-21 | Hitachi Ltd | Method and apparatus for detecting faint fluorescence |
| CN1252586A (en) * | 1998-10-26 | 2000-05-10 | 明碁电脑股份有限公司 | Refrection/peneting type scanner |
| CN2588397Y (en) * | 2002-12-27 | 2003-11-26 | 姜强 | Single light source changeable reflection/transmission integrated film direct light-sensing scanner |
| CN101706434A (en) * | 2009-09-04 | 2010-05-12 | 中国环境科学研究院 | Fluorophototmeter capable of detecting light absorption or light transmission |
| CN105115954A (en) * | 2015-09-11 | 2015-12-02 | 深圳世绘林科技有限公司 | Fluorescence spectrophotometer based on optical integrating sphere |
| CN205229031U (en) * | 2015-12-24 | 2016-05-11 | 北京环宇宏业科技开发有限公司 | Novel colorimetry instrument with detecting pond structure |
| CN205656118U (en) * | 2016-04-29 | 2016-10-19 | 浙江医药高等专科学校 | Double reflection of light colorimetric pool of fluorescence |
| CN205785074U (en) * | 2016-05-24 | 2016-12-07 | 西安工程大学 | The laser facula width of measuring device measured based on Michelson interference |
| CN205719922U (en) * | 2016-05-25 | 2016-11-23 | 广西科技大学 | For measuring cuvette and the optical system thereof of scattering spectrum |
| CN106225715A (en) * | 2016-08-02 | 2016-12-14 | 中国科学院长春光学精密机械与物理研究所 | A kind of pentaprism scanning detection method for non-spherical reflector |
| CN106323873A (en) * | 2016-10-11 | 2017-01-11 | 桂林电子科技大学 | Transmitting/reflecting-combined fluorescent multiplication cuvette |
| CN206772438U (en) * | 2017-04-14 | 2017-12-19 | 中山大学 | Absorption and fluorescence spectrum detecting device based on mobile intelligent terminal |
| CN107748142A (en) * | 2017-09-30 | 2018-03-02 | 南京南瑞集团公司 | A kind of dual-beam based on miniature beam-splitting optical system becomes light path sample spectra analytical equipment |
| CN107807236A (en) * | 2017-10-31 | 2018-03-16 | 南京先进激光技术研究院 | One-color fluorescence detection means |
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