CN109394673A - A kind of preparation method of freckle-removing mask liquid - Google Patents
A kind of preparation method of freckle-removing mask liquid Download PDFInfo
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- CN109394673A CN109394673A CN201811586403.3A CN201811586403A CN109394673A CN 109394673 A CN109394673 A CN 109394673A CN 201811586403 A CN201811586403 A CN 201811586403A CN 109394673 A CN109394673 A CN 109394673A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Abstract
The present invention relates to a kind of preparation method of freckle-removing mask liquid, this method is comprised the steps of: (1) larva of a silkworm with batrytis, Grape Skin, turmeric being mixed and pulverized by the weight ratio of 1 ︰, 1 ︰ 1, the water that 2 times of amounts are then added mixes, and obtains mixed serum；(2) mixed serum is put into fermentor, while accesses bifidobacterium adolescentis and bifidobacterium infantis in the viable count ratio of 1 ︰ 1, and the bacteria concentration in the mixed serum is made to be 1.0 × 107‑1.2×107/ ml, stirs evenly, and SOD enzyme activity of then fermenting by the following method into fermentation liquid is that 1000U/ml-1200U/ml obtains fermentation liquid: after inoculation in first week, setting fermentation temperature is 30 DEG C -32 DEG C, remains that fermentation temperature is 26 DEG C -30 DEG C since the second week；(3) it is 1.06 that relative density at 20 DEG C will be concentrated in vacuo to after fermentation liquid centrifugal filtration；Obtain Fermented Condensed liquid；(4) it is 3000U/ml-3200U/ml that hirudin to hirudin vigor is added into Fermented Condensed liquid, is sieved through filter with 200 mesh pipelines after mixing evenly to get the freckle-removing mask liquid.The freckle effect of facial mask liquid prepared by this method is significant.
Description
Technical field
The present invention relates to cosmetic fields, and in particular to the preparation of the nursing skin containing hirudin.
Background technique
Chloasma (Cholasma) belong to traditional Chinese medicine blackspot, chloasma hepaticum, dusty complexion, butterfly macules scope, be dermal melanin increase and
The common face of one kind of formation is brown or the pigmented skin disease for damaging capacitive of black pigment, is one of dermatology's difficult and complicated illness.
Its volume of being mainly shown as, eyebrow, cheek, upper lip etc. occur that limitation is filbert or brown patch, boundary understand that surface is smooth, more
The women in pregnant woman and irregular menstruation is sent out, and the ratio between female patient and male patient are about 9:1.This disease is in chronic process, the course of disease
Stubbornness, it is touching refractory, patient's beauty is seriously affected, brings many worried and pain to its spirit and life aspect.Disease morbidity
Rate is very high always, and in recent years due to the influence of many factors, in the trend increased year by year, number of going to a doctor is more and more for morbidity.
With the variation and improvement of living standard of people's living environment, chloasma increasingly becomes current medical field, beauty circle is closed jointly
One of note and the hot spot of research.
The pathogenic factor and complicated mechanism of chloasma, with oral contraceptive, inherent cause, oxygen radical accumulation, micro member
Whether the factors such as cellulose content exception, dysfunction of liver are related, still not very clear at present.Modern medicine thinks itself and interior point
It is related to secrete the factors such as imbalance, gestation, the soft nest disease in uterus.Chinese medicine thinks that this disease is mostly dirty in close relations with liver and spleen kidney three, mostly because of liver
Yu Huahuo or kidney deficiency and liver or the dysfunction of the spleen in transport, lead to stagnation of the circulation of vital energy extravasated blood, and qi and blood cannot go up honor caused by face.Currently, most researchs
Thinking, chloasma is since patient with chloasma cutaneous lesion melanocyte activity enhances, and melanocyte and melanosome increase, into
And color spot is showed, under pathological state, intradermal macrophage, which can swallow, is dropped into corium melanosome, or calmness is on corium
Layer, or degrade in the cell, a part is through Lymph Node Metastasis.The generation of pigment and excretion disequilibrium, and superfluous it is gathered in table
Part in chrotoplast just forms pigmentation.The excretion of melanin has two approach: first is that drained out of kidney, it is another be from
Skin discharge, i.e., melanin is transferred in keratoprotein, is migrated to cuticula with epidermal growth, is finally changed with the cuticula period
Newly fall off.The principle of Modern medical therapy chloasma mainly inhibits melanocyte activity, inhibits B16 cell, removal
Melanin, destruction melanin granule and the utilization generated has opacifier of anti-luminous effect etc..Mainly have at present following several
Kind treatment method:
(1) it takes orally or vein gives vitamin C, while vitamin E being cooperated to use, the oxidation that melanin can be prevented to be metabolized
Journey inhibits melanin to be formed.
(2) local topical Hydrochinone Cream inhibits the formation of melanin granule and increases its degradation, inhibits melanocyte DNA
With the synthesis of RNA, melanocyte is not influenced, treating epidermis type and mixed type chloasma in conjunction with wide spectrum opacifier has a constant current modulation
Effect or local topical retinoid cream can inhibit the melanoma cell tyrosinase activity of in vitro culture, prevent melanin to cutin
The transhipment of confluent monolayer cells, while reducing cutin and being formed, increase Medicated Permeation.Although above-mentioned clinic Western medicine has one to treatment chloasma
Fixed curative effect, but effect is not often good enough, and there are different degrees of side effect, prolonged application easily causes serious complication.So
And traditional Chinese medicine has complete therapy theory system and clinical experience abundant in terms for the treatment of chloasma, there is integrated regulation internal organs function
Energy unique advantage, therefore traditional Chinese medicine has wide prospect in terms for the treatment of chloasma.
Melanin is to generate DOPA by colourless tyrosine under the action of tyrosinase, and DOPA is again in the work of tyrosinase
Become DOPA quinone under, then through a series of oxidation process, eventually forms melanin.Under normal circumstances, after melanin is formed, one
Part can be decomposed, and be excreted by kidney, another part can fall off with falling off for epidermis.But if tyrosine is living
Power enhancing, melanin increases, the melanin of surge again because metabolism it is slow due to can not excrete, will deposit and be formed on the face
Spot.Spot is vital organs of the human body dysfunction, derangement of Qi and blood, stagnation of QI due to depression of the liver, qi depression to blood stasis, the unsmooth and endocrine of qi-blood-body fluid circulation
It lacks of proper care in the reflection of face.Middle treatment spot treating both manifestation and root cause of disease is reached from Traditional Chinese Medical Five Viscera Treatment by adjusting qi-blood-body fluid in the hope of Yin Yang balancing
To the purpose of spot-removing beautifying face, chloasma is prevented to recur.
Currently, clinical treatment chloasma is mostly used with oxidation resistant polyphenol, the biological anti-oxidants such as saponin are main
The externally used coating medicine of ingredient, cure rate is low, and high recurrence rate, curative effect is unobvious, and medication is inconvenient.Fail flat from endocrine is adjusted
Weighing apparatus, boosts metabolism, and the human body internal factor of oxidation and removing free radicals combines antifatigue principle to carry out health therapy.Therefore
The prior art is urgently improved and is developed.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of preparation method of freckle-removing mask liquid, face prepared by this method
The freckle effect of film liquid is significant.
The technical solution that the present invention solves the above problems is as follows:
A kind of preparation method of freckle-removing mask liquid, this method comprise the steps of:
(1) larva of a silkworm with batrytis, Grape Skin, turmeric are mixed and is pulverized by the weight ratio of 1 ︰, 1 ︰ 1,2 times of amounts are then added
Water mixes, and obtains mixed serum；
(2) mixed serum is put into fermentor, while accesses bifidobacterium adolescentis and baby couple in the viable count ratio of 1 ︰ 1
Qi bacillus, and the bacteria concentration in the mixed serum is made to be 1.0 × 107-1.2×107/ ml, stirs evenly, then by following
Method ferment into fermentation liquid SOD enzyme activity be 1000U/ml-1200U/ml obtain fermentation liquid: after inoculation in first week, setting fermentation
Temperature is 30 DEG C -32 DEG C, remains that fermentation temperature is 26 DEG C -30 DEG C since the second week；
(3) it is 1.06 that relative density at 20 DEG C will be concentrated in vacuo to after fermentation liquid centrifugal filtration；Obtain Fermented Condensed liquid；
(4) it is 3000U/ml-3200U/ml that hirudin to hirudin vigor is added into Fermented Condensed liquid, after mixing evenly
Filter is sieved through with 200 mesh pipelines to get the freckle-removing mask liquid.
It is raw materials used in above scheme to be respectively provided with following characteristic:
Leech used is the drug that activates blood circulation and disperses blood clots in Chinese Traditional Medicine.Hirudin (hirudin) is its principle active component,
It is the single chain polypeptide being made of 65-66 amino acid.Hirudin used in the present invention is by the limited public affairs of Guangzhou leech peptide element medical sci-tech
Department's production, with living body hiruto, UF membrane is formed after digesting, and since molecular weight is small (below 7000 dalton), is had to skin
Good permeability, and vigor can quantitative detection, hirudin vigor is in 300,000 U-32, ten thousand U/ml, and effect is than Chinese medicine solid carbon dioxide leech
It is more powerful.
The larva of a silkworm with batrytis: stiff for natural infection (or artificial infection) muscardine Beauveria bassiana (Bals) Vuill
The full worm of dead drying, abbreviation bombyx batryticatus.The larva of a silkworm with batrytis as traditional Chinese medicine, pharmaceutical research find the larva of a silkworm with batrytis have it is antibacterial, anticonvulsion,
Anticoagulant, antithrombotic promotees the drug effects such as fibrinolytic, tumor suppression, hypnosis.It is recorded on Shennong's Herbal, the larva of a silkworm with batrytis has that " go out blackspot, makes us face
Color is good " the effect of.The larva of a silkworm with batrytis contains amino acid and active mercerising element substance ammonium oxalate, nutritious skin and beautification function.Bombyx batryticatus
The contained irritating upper sebaceous glands of protein, the effect of modulability hormone secretion, thus to caused by female sex hormone secretion imbalance
Chloasma has certain curative effect.The larva of a silkworm with batrytis also contains the hydrolases such as protease, chitinase, lipase, can digest cutin, decomposes pigment,
Anti- scar, the effect gone acne, control ecchymosis.Skin Cell can be promoted newborn, adjust sebum, improve skin microcirculation, can brighten
It is sun-proof, pigmentation is eliminated, skin elasticity is kept.Turmeric China medicine thinks；Turmeric nature and flavor are pungent, bitter, warm-natured, return heart, spleen, liver
Had effects that blood-breaking, promoting the circulation of qi, promoting menstruation, analgesic.Abdominal mass, women's blood stasis, the postpartum stasis of blood stop abdominal pain, carbuncle swells.Turmeric evidence
" Bencao Jingshu ": turmeric, taste bitter flavor prevailing over pungent flavor is bad, and Xin Xiang is dry strong, and property should not tremble with fear.Hardship can expel the heat-evil, pungent energy dissipating bind, therefore main trusted subordinate ties product
Category blood system person.And gas can be controlled, therefore Yun Xiaqi again.
Grape Skin: also containing another kind, polyphenoils --- both effectiveness ingredient procyanidine is to send out so far in Grape Skin
One of existing most strong effective free radical scavenger.It carries out in experiment in vivo research in mouse, is induced with procyanidine, VC, VE
Liver, the protective effect of lipid peroxidation compares in brain tissue, more can be to oxidative damage after discovery Grape Skin is fermented
It plays a protective role, and protective effect is better than other antioxidants and other effects.
Contained curcumin can promote the generation of transforming growth factor β and some collagens, epidermal cell again in turmeric
It is raw, and reduce the injury of free radical；Curcumin is recognized as the curative effect that can have improvement to some skin diseases, and makes to ultraviolet light
At cutaneous lesions have protective effect, because curcumin have it is anti-oxidant and remove free radical ability, can capture ultraviolet light photograph
Caused free radical, under attack to avoid cell when penetrating.
Common crystal mask or non-woven fabrics facial mask can be further made in freckle-removing mask liquid of the present invention.
Freckle-removing mask liquid of the present invention has the advantages that
1) it is using the bifidobacterium adolescentis of food-grade and the double qi bars of baby that fermentation liquid preparation provided by the invention, which is method,
For the mixed bacteria of bacterium as fermenting microbe, it is high that fermentation generates the more other strains of SOD enzyme activity.
2) the food-grade fermenting microbe that fermentation liquid preparation provided by the invention uses is not in bacterium during the fermentation
The problem of with virus pollution, solves the safety problem of fermentation liquid；
3) facial mask liquid provided by the invention is to be fermented using the larva of a silkworm with batrytis, Grape Skin, turmeric by probiotics, makes white deadlock
Amino acid contained and active mercerising element substance ammonium oxalate in silkworm, contained bioactivity ingredient procyanidine in Grape Skin, turmeric
In contained curcumin, functional group is more active after fermentation, can with fermentation simultaneously generation SOD enzyme and addition hirudin, together
Corium and subcutaneous tissue can be acted on, freckle effect is reached effectively through the infiltration of pore.
Specific embodiment
Embodiment 1
1, freckle-removing mask liquid is prepared
(1) larva of a silkworm with batrytis 5kg, Grape Skin 5kg and turmeric 5kg are taken, is pulverized into 400 mesh, 30kg water is then added and mixes,
Obtain mixed serum；
(2) mixed serum is put into fermentor, while accesses bifidobacterium adolescentis and baby couple in the viable count ratio of 1 ︰ 1
Qi bacillus, and the bacteria concentration in the mixed serum is made to be 1.15 × 107/ ml, stirs evenly, and then ferments by the following method
Into fermentation liquid, SOD enzyme activity is that 1150U/ml obtains fermentation liquid: after inoculation in first week, setting fermentation temperature is 31 DEG C, from second
Start in week to remain that fermentation temperature is 28 DEG C；
(3) it is 1.06 that relative density at 20 DEG C will be concentrated in vacuo to after fermentation liquid centrifugal filtration；Obtain Fermented Condensed liquid；
(4) it is 3100U/ml that hirudin to hirudin vigor is added into Fermented Condensed liquid, after mixing evenly with 200 mesh pipes
Road is sieved through filter to get the freckle-removing mask liquid.
2, freckle-removing mask (for 10 facial masks) are prepared:
The ratio that 80mL freckle-removing mask liquid is carried in every facial mask takes freckle-removing mask liquid that scattered propylene glycol 40g, card is added
Glue 8.8g and ethylparaben 1.6g is drawn, 80-90 DEG C is heated to and is sufficiently stirred 15 minutes, pH value is adjusted to 5.5-6.5, is cooled to
It about 50 DEG C, mixes and dissolves, then form a film, pack, the freckle-removing mask to sterilize to get specification for 10 × 15cm.
Embodiment 2
1, freckle-removing mask liquid is prepared
(1) larva of a silkworm with batrytis 5kg, Grape Skin 5kg and turmeric 5kg are taken, is pulverized into 400 mesh, 30kg water is then added and mixes,
Obtain mixed serum；
(2) mixed serum is put into fermentor, while accesses bifidobacterium adolescentis and baby couple in the viable count ratio of 1 ︰ 1
Qi bacillus, and the bacteria concentration in the mixed serum is made to be 1.2 × 107/ ml, stirs evenly, and then ferments by the following method
Into fermentation liquid, SOD enzyme activity is that 1200U/ml obtains fermentation liquid: after inoculation in first week, setting fermentation temperature is 32 DEG C, from second
Start in week to remain that fermentation temperature is 30 DEG C；
(3) it is 1.06 that relative density at 20 DEG C will be concentrated in vacuo to after fermentation liquid centrifugal filtration；Obtain Fermented Condensed liquid；
(4) it is 3000U/ml that hirudin to hirudin vigor is added into Fermented Condensed liquid, after mixing evenly with 200 mesh pipes
Road is sieved through filter to get the freckle-removing mask liquid.
2, freckle-removing mask (for 10 facial masks) are prepared:
The ratio that 80mL freckle-removing mask liquid is carried in every facial mask takes freckle-removing mask liquid that scattered propylene glycol 40g, card is added
Glue 8.8g, ethylparaben 1.6g and 0.5% hyalomitome acid solution 10ml are drawn, 80-90 DEG C is heated to and is sufficiently stirred 15 minutes, is adjusted
PH value is cooled to about 50 DEG C, adds, mix and dissolve, then form a film, pack, sterilizing is 10 to get specification to 5.5-6.5
The freckle-removing mask of × 15cm.
Embodiment 3
1, freckle-removing mask liquid is prepared
(1) larva of a silkworm with batrytis 5kg, Grape Skin 5kg and turmeric 5kg are taken, is pulverized into 400 mesh, 30kg water is then added and mixes,
Obtain mixed serum；
(2) mixed serum is put into fermentor, while accesses bifidobacterium adolescentis and baby couple in the viable count ratio of 1 ︰ 1
Qi bacillus, and the bacteria concentration in the mixed serum is made to be 1.0 × 107/ ml, stirs evenly, and then ferments by the following method
Into fermentation liquid, SOD enzyme activity is that 1000U/ml obtains fermentation liquid: after inoculation in first week, setting fermentation temperature is 30 DEG C, from second
Start in week to remain that fermentation temperature is 26 DEG C；
(3) it is 1.06 that relative density at 20 DEG C will be concentrated in vacuo to after fermentation liquid centrifugal filtration；Obtain Fermented Condensed liquid；
(4) it is 3200U/ml that hirudin to hirudin vigor is added into Fermented Condensed liquid, after mixing evenly with 200 mesh pipes
Road is sieved through filter to get the freckle-removing mask liquid.
2, freckle-removing mask is prepared
2, freckle-removing mask (for 10 facial masks) are prepared:
The ratio that 80mL freckle-removing mask liquid is carried in every facial mask takes freckle-removing mask liquid that scattered propylene glycol 40g, card is added
It draws glue 8.8g, ethylparaben 1.6g and collagen 2g to be heated to 80-90 DEG C and be sufficiently stirred 15 minutes, adjusts pH value to 5.5-
6.5, about 50 DEG C are cooled to, mixes and dissolves, then form a film, is packed, the freckle-removing mask to sterilize to get specification for 10 × 15cm.
Embodiment 4 (clinical effectiveness research)
According to " health food examine with assessment technique specification (version in 2003) " auxiliary beauty treatment and eliminating spot functional check method into
Row human experimentation.
1, subject is included in standard
Meet the trial volunteer of following condition through physical examination:
1.1 faces are filbert to dark brown, the patch of boundary clear, usual symmetry distribution, no inflammatory performance and the scales of skin that peel off.
1.2 without obvious subjective symptoms.
After 1.3 occur mainly in puberty, women is multiple.
1.4 state of an illness have certain seasonality, and Xia Chongdong is light.
1.5 without obvious endocrine system disease, and excludes pigmentation caused by Other diseases.
2, Subject Exclusion Criteria.
2.1 ages in 18 years old or less and over-65s, pregnant occasion and breast feeding women, allergic constitution.
2.2 are associated with the serious diseases such as angiocarpy, the cerebrovascular, liver, kidney and hemopoietic system and endocrine system disease, cyclothymic
2.3 wine-head of person or smoker.
2.4 take article related with tested function in a short time, influence the judgement to result.
2.5 do not take given the test agent by regulation, can not determine that effect or data do not affect the treatment or safety judgement person entirely.
3, case source and selection
Female subjects 120 that meet conditions above from Shantou Hospital of Skin Diseases outpatient service are selected by the principle of voluntariness,
In, the age is 23 people of 19-34, and the age is 51 people of 35-44, and the age is 46 people of 55-64.By the chloasma face of subject
Color, area situation are randomly divided into two groups, wherein 60 people of experimental group, 60 people of control group.
4, test medicine and treatment method
Experimental group uses the facial mask of above-described embodiment 1, and patient lies supine spreads on face after washing one's face, and uses facial mask 1 every time
Piece 1 time every other day, is thrown off and is cleaned, continuous 30 days 15 notes after each 15min.Subject has stopped using in the trial employment period and has supported
Change spot product, drug and the health food of face nti-freckle.
Control group, which uses, is added without the freckle-removing mask liquid and by blank facial mask made of the method for above-described embodiment 1, patch
Coating method and other conditions are identical with above-mentioned experimental group.
5, efficiency observation
The detection of Head And Face chloasma
5.1. size changes
With the long diameter and wide diameter of the same chloasma in the tested front and back of tape measure, reference area (cm2)。
Area of chloasma size variation the results are shown in Table 1
Table 1
| Group arrives | Number of cases | (mm before use2) | (mm after use2) | Difference (mm2) |
| Experimental group | 60 | 6121±2373 | 4963±1680## | 1258±693** |
| Control group | 57 | 6173±2219 | 6049±2091 | 124±365 |
Note: * * p < 0.01 compared with the control group, ## with use p < 0.01 compared with preceding
The variation of 5.2 shades
It is Huang according to brown (Y+M+Bk is yellow+pinkish red+black folded color) colour atla in " practical standard colour atla " (first edition)
The judgment criteria of foxiness shade: I degree (15,20,5), II degree (30,40,10), III degree (40,60,15).
The variation of the chloasma color depth the results are shown in Table 2
Table 2
| Group arrives | Number of cases | Before use | After use | Difference |
| Experimental group | 60 | 2.36±0.44 | 2.06±0.30# | -0.30±0.19* |
| Control group | 57 | 2.30±0.45 | 2.29±0.56 | -0.01±0.11 |
Note: * p < 0.01 compared with the control group, # with use p < 0.01 compared with preceding
6, spot-removal function determines
Effective: chloasma color declines II degree or area reduces >=30%, does not generate new chloasma.
Effective: chloasma color declines I degree or area diminution value S and exists: 30% > S >=10%, and does not generate new yellowish-brown
Spot.
Invalid: area of chloasma reduces < 10%, and color is without significant change.
Effect judge the results are shown in Table 3
3 treatment group of table and control group curative effect compare
| Group | Case load | It is effective | Effectively | In vain | Efficient % |
| Treatment group | 60 | 17 | 38 | 5 | 91.7 |
| Control group | 57 | 0 | 0 | 57 | 0 |
Note: control group is detached from 3.
7, conclusion:
The area of chloasma of 60 chloasma subjects averagely reduces 1258 ± 693mm2(P < 0.01) lighter, colour atla
0.30 ± 0.19 degree of (P < 0.01) example is averagely reduced, wherein there is (aobvious) effect 55, effective percentage 91.7% illustrates this functions of removing chloasma face
The significant effect of facial mask functions of removing chloasma prepared by film liquid.
Claims (1)
1. a kind of preparation method of freckle-removing mask liquid, this method comprise the steps of:
(1) larva of a silkworm with batrytis, Grape Skin, turmeric are mixed and is pulverized by the weight ratio of 1 ︰, 1 ︰ 1, the water that 2 times of amounts are then added is mixed
It is even, obtain mixed serum；
(2) mixed serum is put into fermentor, while accesses bifidobacterium adolescentis and the double qi bars of baby in the viable count ratio of 1 ︰ 1
Bacterium, and the bacteria concentration in the mixed serum is made to be 1.0 × 107-1.2×107/ ml, stirs evenly, then by the following method
Fermentation SOD enzyme activity into fermentation liquid is that 1000U/ml-1200U/ml obtains fermentation liquid: after inoculation in first week, fermentation temperature is arranged
It is 30 DEG C -32 DEG C, remains that fermentation temperature is 26 DEG C -30 DEG C since the second week；
(3) it is 1.06 that relative density at 20 DEG C will be concentrated in vacuo to after fermentation liquid centrifugal filtration；Obtain Fermented Condensed liquid；
(4) it is 3000U/ml-3200U/ml that hirudin to hirudin vigor is added into Fermented Condensed liquid, is used after mixing evenly
200 mesh pipelines are sieved through filter to get the freckle-removing mask liquid.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113520898A (en) * | 2021-09-17 | 2021-10-22 | 汇泰渤海水产有限责任公司 | Application of hirulog HE-D in skin care and whitening |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102847030A (en) * | 2012-08-21 | 2013-01-02 | 青岛文创科技有限公司 | Freckle-removal facial mask |
| CN107468999A (en) * | 2017-08-25 | 2017-12-15 | 广州蛭肽素医药科技有限公司 | A kind of fermentation composition with beauty treatment and eliminating spot function and preparation method thereof |
| CN108618975A (en) * | 2018-07-03 | 2018-10-09 | 崔洪学 | A kind of Chinese medicine compound prescription facial masks for whitening faces and removing spots and preparation method thereof |
-
2018
- 2018-12-25 CN CN201811586403.3A patent/CN109394673A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102847030A (en) * | 2012-08-21 | 2013-01-02 | 青岛文创科技有限公司 | Freckle-removal facial mask |
| CN107468999A (en) * | 2017-08-25 | 2017-12-15 | 广州蛭肽素医药科技有限公司 | A kind of fermentation composition with beauty treatment and eliminating spot function and preparation method thereof |
| CN108618975A (en) * | 2018-07-03 | 2018-10-09 | 崔洪学 | A kind of Chinese medicine compound prescription facial masks for whitening faces and removing spots and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113520898A (en) * | 2021-09-17 | 2021-10-22 | 汇泰渤海水产有限责任公司 | Application of hirulog HE-D in skin care and whitening |
| CN113520898B (en) * | 2021-09-17 | 2021-12-21 | 汇泰渤海水产有限责任公司 | Application of hirulog HE-D in skin care and whitening |
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