CN109384596A - The preparation method of fertilizer is immunized in a kind of crop disease-resistant - Google Patents

The preparation method of fertilizer is immunized in a kind of crop disease-resistant Download PDF

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Publication number
CN109384596A
CN109384596A CN201811521042.4A CN201811521042A CN109384596A CN 109384596 A CN109384596 A CN 109384596A CN 201811521042 A CN201811521042 A CN 201811521042A CN 109384596 A CN109384596 A CN 109384596A
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China
Prior art keywords
fertilizer
immunized
preparation
crop disease
prepared
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CN201811521042.4A
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Chinese (zh)
Inventor
丁伟
程茁
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Northeast Agricultural University
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Northeast Agricultural University
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Priority to CN201811521042.4A priority Critical patent/CN109384596A/en
Publication of CN109384596A publication Critical patent/CN109384596A/en
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00Other organic fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/30Layered or coated, e.g. dust-preventing coatings
    • C05G5/38Layered or coated, e.g. dust-preventing coatings layered or coated with wax or resins

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Fertilizers (AREA)

Abstract

The preparation method of fertilizer is immunized in a kind of crop disease-resistant, belongs to crop technical field of fertilizers.It by lactic acid bacteria, bacillus and visits bad mould and cultivates respectively, be mixed to form mixed bacteria liquid a later and filter, xanthan gum, polyvinyl alcohol resin, tea seed powder and burnt sugar coloring is added, film forming bacterium solution b is prepared into after stirring, it is spare;It takes organic matter and nitrogen, phosphorus, potassium, silicon, calcium, magnesium, iron, boron and inertia viscous material mixed in proportion, is prepared into material c, material c is sprayed water and squeezed;By the material c polishing after extruding, it is put into high temperature drying equipment, screens out material particles c1 later;Material particles c1 is transmitted in rotary drum agitator tank, spare film forming bacterium solution b is added, is uniformly mixed, is prepared into coated granule d;Coated granule d is dried, anti pathologic immunity fertilizer is prepared into;The present invention solves the problems, such as that existing immune formulation has a single function.

Description

The preparation method of fertilizer is immunized in a kind of crop disease-resistant
Technical field
The invention belongs to crop technical field of fertilizers, and in particular to the preparation method of fertilizer is immunized in a kind of crop disease-resistant.
Background technique
When plant by environmental stimuli or be in adverse circumstances condition when, can by adjust itself defence and metabolic system produce Raw immune response, this defense reaction or the immune resistance reaction of plant, can make plant postpone or mitigate disease generation and Development reduces the usage amount of chemical pesticide and chemical fertilizer, to reduce the pesticide residue of agricultural product.However, most of initiative, The basic principle of control plant disease drug is all using pathogen as target, then can quickly kill the drug of target comprehensively into one Step is developed into pesticide by agriculture chemical registration requirement, ignores the host plant itself endangered by pathogen to adventive resistivity Utilization.
Plant immune inducer (or referred to as plant vaccine) is the vaccine engineering technology after vaccine for man, animal vaccine The frontier of appearance, and the research direction that the current initiative of biological pesticide in the world is more popular.Inducer is immunized in appliable plant Although there is no direct killing effect to crop disease and insect, is generated and resisted by induction or activated plant by exogenous biological or molecule Property substance, can to certain pathogens generate resistance or inhibit germ growth.After it is applied on crop, by inducing crop Generation is resisted or the substance of prevention and control crop disease, to achieve the purpose that prevent and treat crop disease.
According to incompletely statistics, the current country has been lured in the plant immune that the Institute for the Control of Agrochemicals of the Ministry of Agriculture,PRC obtains formal registration anti- Agent mainly has the types of drugs such as protein elicitor, beneficial germ, chitosan oligosaccharide and abscisic acid.Research in relation to plant immune inducer With utilization, approval of the government in relation to management and popularizing department and vast farmers has also been obtained at present, protection plant health is raw Increasingly important role is played in terms of long, guarantee food and ecological safety.Plant immune inducer, which will be expected to become, to be had The novel strategic industry of great development prospect.
However, a series of immune formulations currently developed in succession in agricultural production, are required to before crop falls ill It is used alone.It dresses seed, soak seed, pouring root and foliage-spray is the common application method of immune formulation, although can effectively prevent to make The generation of object disease, but usually cause crop growth bad since nutrient is insufficient.Therefore, immune substance and crop is raw Nutrient required for long development combines the immune fertilizer to form crop, while immune disease-resistance and meets crop to nutrient Demand can promote the growth of crop root and ground position again while reaching diseases prevention, output increased, quality improving to crop Play a significant role with chemical pesticide is reduced to the pollution of natural environment, is an innovation of medicine fertilizer research field.
Summary of the invention
The purpose of the present invention is to provide the preparation methods that fertilizer is immunized in a kind of crop disease-resistant, to solve to deposit in the prior art Immune formulation have a single function and existing immune formulation needs and Fertilizer ratio is using could provide nutrient needed for crop Problem realizes applied once and obtains two kinds of effects, not only prevention of damage by disease, but also nutrient needed for providing crop, save labour Cost, it is convenient and efficient.
Realize that above-mentioned purpose, the present invention take following technical proposals: the preparation method of fertilizer, packet is immunized in a kind of crop disease-resistant Include following steps:
(1) it by lactic acid bacteria, bacillus and visits bad mould difference fermented and cultured and is weighed later by mass fraction 1-5:1-5:1-4 The lactic acid bacteria, bacillus and bad mould is visitd, and is mixed;- 48h forms mixed bacteria liquid a for 24 hours for culture, and described is mixed Immune substance is turned out in combined bacteria liquid a;
(2) the filtering mixed bacteria liquid a, be added into filtered mixed bacteria liquid a ‰ -5wt. ‰ of 1wt. xanthan gum, The burnt sugar coloring of ‰ -2wt. ‰ of ‰ -2wt. of 1wt., ‰ polyvinyl alcohol resin, the tea seed powder of ‰ -3wt. ‰ of 1wt. and 12wt. passes through Film forming bacterium solution b is prepared into after high speed agitator stirring, it is spare;
(3) it takes organic matter and nitrogen, phosphorus, potassium, silicon, calcium, magnesium, iron, boron and inertia viscous material mixed in proportion, is prepared into material c, Wherein, the content of organic matter is 25wt.%-40wt.%, and the total content of nitrogen, phosphorus and potassium is 5wt.%-10wt.%, silicon, calcium, magnesium, iron And the total content of boron is 24wt.%-44wt.%, remaining is inertia viscous material;
(4) it sprays water to the material c, makes the water content 15%-20% of the material c, later, squeezed through Squeezinggranulator Pressure;Material c after extruding is polished by high speed rotation polishing machine, later, is put into high temperature drying equipment, after dried Material c be sent to screen(ing) machine and screen out material particles c1;
(5) the material particles c1 is transmitted in rotary drum agitator tank, the film forming bacterium solution b in step (2) is added, stirring is mixed It closes uniformly, is prepared into coated granule d;
(6) the coated granule d is blown into 50 DEG C of -60 DEG C of hot-air seasonings by rotary drum dryer, until the water content of coated granule d For 8%-10%, it is prepared into anti pathologic immunity fertilizer.
Compared with prior art, the beneficial effects of the present invention are: fertilizer is immunized compared with immune formulation in crop disease-resistant, have Unique superiority, is mainly reflected in:
1, crop disease-resistant is induced, while supplementing the necessary nutrient of crop;
2, applied once realizes the disease-resistant of crop and promotes two effects of plant growth;
3, it solves the problems, such as that immune formulation is used alone only with prophylaxis effect, and needs to promote with Fertilizer ratio use The individual event function of crop production;
4, crop disease-resistant is immunized fertilizer and is combined using complex immunity substance with fertilizer, is improved again while crop disease-resistant feeding The absorption and utilization rate divided;
5, the potting of various crop and field experiment show that fertilizer is immunized to the control efficiency of disease in this crop disease-resistant for many years It is 80%~90%, improves crop yield 15%~30%, fertilizer utilization efficiency increases by 10%~20%.
Specific embodiment
The technical solution in the present invention is clearly and completely described below, it is clear that described embodiment is only hair Bright a part of the embodiment, instead of all the embodiments, based on the embodiments of the present invention, those of ordinary skill in the art exist Every other embodiment obtained under the premise of creative work is not made, shall fall within the protection scope of the present invention.
Specific embodiment 1: the present invention discloses the preparation method that fertilizer is immunized in a kind of crop disease-resistant, including following step It is rapid:
(1) it by lactic acid bacteria, bacillus and visits bad mould and distinguishes fermented and cultured, fermentor, which leads to 121 DEG C of steam, empty eliminates bacterium 30- 40min is separately added into wheat bran, beans by mass fraction 10-20:1-5:1-3:0.01-0.02 in fluid nutrient medium per ton after cooling Powder, white sugar, NaCl, remaining is supplemented to 100% with water, and then 121 DEG C of sterilizing 40-60min, each fermentor are inoculated with respectively Lactic acid bacteria, bacillus and the Bai Lai mould of ‰ -2 wt. ‰ of 1wt., under the conditions of 28-30 DEG C, ventilatory capacity 20-50mL/ Min, agitated machine (revolving speed 120r/min) stirring, bacillus and lactic acid bacteria are cultivated 2-3 days, visit bad mould culture 4-7 days Later, the lactic acid bacteria, bacillus are weighed by mass fraction 1-5:1-5:1-4 and visits bad mould, and mixed, Under the conditions of 28-30 DEG C, 10min is stirred at interval of 2-8h, is cultivated in -48h formation for 24 hours mixed bacteria liquid a, the mixed bacteria liquid a Turn out a large amount of immune substances;The immune substance is mainly polypeptide and β -1,3 glucan;
(2) the filtering mixed bacteria liquid a, be added into filtered mixed bacteria liquid a ‰ -5wt. ‰ of 1wt. xanthan gum, The burnt sugar coloring of ‰ -2wt. ‰ of ‰ -2wt. of 1wt., ‰ polyvinyl alcohol resin, the tea seed powder of ‰ -3wt. ‰ of 1wt. and 12wt. passes through Film forming bacterium solution b is prepared into after high speed agitator stirring, it is spare;
(3) it takes organic matter and nitrogen, phosphorus, potassium, silicon, calcium, magnesium, iron, boron and inertia viscous material mixed in proportion, is prepared into material c, Wherein, the content of organic matter is 25wt.%-40wt.%, and the total content of nitrogen, phosphorus and potassium is 5wt.%-10wt.%, silicon, calcium, magnesium, iron And the total content of boron is 24wt.%-44wt.%, remaining is inertia viscous material;
(4) it sprays water to the material c, makes the water content 15%-20% of the material c, later, squeezed through Squeezinggranulator Pressure;Material c after extruding is polished by high speed rotation polishing machine, later, is put into high temperature drying equipment, after dried Material c be sent to screen(ing) machine and screen out material particles c1;
(5) the material particles c1 is transmitted in rotary drum agitator tank, the film forming bacterium solution b in step (2) is added, stirring is mixed It closes uniformly, is prepared into coated granule d;
(6) the coated granule d is blown into 50 DEG C of -60 DEG C of hot-air seasonings by rotary drum dryer, until the water content of coated granule d For 8%-10%, it is prepared into anti pathologic immunity fertilizer.
Specific embodiment 2: present embodiment is the further explanation made to specific embodiment one, step (1) In, it the lactic acid bacteria, bacillus and visits bad mould and is configured and mixed by mass fraction 2:2:1.
Specific embodiment 3: present embodiment is the further explanation made to specific embodiment one, step (2) In, the revolving speed of the high speed agitator is 1000r/min-2000r/min.
Specific embodiment 4: present embodiment is the further explanation made to specific embodiment one, step (3) In, wherein the mass ratio of the nitrogen, phosphorus and potassium is 3-6:1-2:1-2, the silicon, calcium, magnesium, iron and boron mass ratio be 15-25:5-10:2-5:1-2:1-2.
Specific embodiment 5: present embodiment is the further explanation made to specific embodiment one, step (4) In, the pressure that the Squeezinggranulator squeezes is 10N-12N.
Specific embodiment 6: present embodiment is the further explanation made to specific embodiment one, step (4) In, the revolving speed of the high speed rotation polishing machine is 1000r/min-2000r/min.
Specific embodiment 7: present embodiment is the further explanation made to specific embodiment one, step (4) In, the high temperature drying temperature is 300 DEG C -350 DEG C, and the high temperature drying time is 0.5min-1min.
Specific embodiment 8: present embodiment is the further explanation made to specific embodiment one, step (4) In, the material particles c1 diameter is 2mm-4mm.
Specific embodiment 9: present embodiment is the further explanation made to specific embodiment one, step (5) In, the film forming bacterium solution b of 5kg-10kg is added in material particles c1 described in every 1000kg.
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie In the case where without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in the form of others dress body.Therefore, no matter From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power Benefit requires rather than above description limits, it is intended that by all in the meaning and scope for the condition of equivalent for falling in claim Variation is included within the present invention.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art The other embodiments being understood that.

Claims (9)

1. the preparation method that fertilizer is immunized in a kind of crop disease-resistant, which comprises the following steps:
By lactic acid bacteria, bacillus and visits bad mould difference fermented and cultured and weigh institute by mass fraction 1-5:1-5:1-4 later The lactic acid bacteria stated, bacillus and bad mould is visitd, and is mixed;- 48h forms mixed bacteria liquid a, the mixing for 24 hours for culture Immune substance is turned out in bacterium solution a;
The filtering mixed bacteria liquid a, be added into filtered mixed bacteria liquid a ‰ -5wt. ‰ of 1wt. xanthan gum, The burnt sugar coloring of ‰ -2wt. ‰ of ‰ -2wt. of 1wt., ‰ polyvinyl alcohol resin, the tea seed powder of ‰ -3wt. ‰ of 1wt. and 12wt. passes through Film forming bacterium solution b is prepared into after high speed agitator stirring, it is spare;
It takes organic matter and nitrogen, phosphorus, potassium, silicon, calcium, magnesium, iron, boron and inertia viscous material mixed in proportion, is prepared into material c, In, the content of organic matter is 25wt.%-40wt.%, and the total content of nitrogen, phosphorus and potassium is 5wt.%-10wt.%, silicon, calcium, magnesium, iron and The total content of boron is 24wt.%-44wt.%, remaining is inertia viscous material;
It sprays water to the material c, squeezes the water content 15%-20% of the material c through Squeezinggranulator later;It will Material c after extruding is polished by high speed rotation polishing machine, later, is put into high temperature drying equipment, will be dried after object Material c is sent to screen(ing) machine and screens out material particles c1;
The material particles c1 is transmitted in rotary drum agitator tank, the film forming bacterium solution b in step (2) is added, is stirred Uniformly, it is prepared into coated granule d;
The coated granule d is blown into 50 DEG C of -60 DEG C of hot-air seasonings by rotary drum dryer, until the water content of coated granule d is 8%-10% is prepared into anti pathologic immunity fertilizer.
2. the preparation method that fertilizer is immunized in a kind of crop disease-resistant according to claim 1, it is characterised in that: in step (1), It the lactic acid bacteria, bacillus and visits bad mould and is configured and mixed by mass fraction 2:2:1.
3. the preparation method that fertilizer is immunized in a kind of crop disease-resistant according to claim 1, it is characterised in that: in step (2), The revolving speed of the high speed agitator is 1000r/min-2000r/min.
4. the preparation method that fertilizer is immunized in a kind of crop disease-resistant according to claim 1, it is characterised in that: in step (3), Wherein, the mass ratio of the nitrogen, phosphorus and potassium is 3-6:1-2:1-2, the silicon, calcium, magnesium, iron and boron mass ratio be 15- 25:5-10:2-5:1-2:1-2.
5. the preparation method that fertilizer is immunized in a kind of crop disease-resistant according to claim 1, it is characterised in that: in step (4), The pressure that the Squeezinggranulator squeezes is 10N-12N.
6. the preparation method that fertilizer is immunized in a kind of crop disease-resistant according to claim 1, it is characterised in that: in step (4), The revolving speed of the high speed rotation polishing machine is 1000r/min-2000r/min.
7. the preparation method that fertilizer is immunized in a kind of crop disease-resistant according to claim 1, it is characterised in that: in step (4), The high temperature drying temperature is 300 DEG C -350 DEG C, and the high temperature drying time is 0.5min-1min.
8. the preparation method that fertilizer is immunized in a kind of crop disease-resistant according to claim 1, it is characterised in that: in step (4), The material particles c1 diameter is 2mm-4mm.
9. the preparation method that fertilizer is immunized in a kind of crop disease-resistant according to claim 1, it is characterised in that: in step (5), The film forming bacterium solution b of the addition of material particles c1 described in every 1000kg 5kg-10kg.
CN201811521042.4A 2018-12-12 2018-12-12 The preparation method of fertilizer is immunized in a kind of crop disease-resistant Pending CN109384596A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110063364A (en) * 2019-05-15 2019-07-30 福建省亚热带植物研究所 A kind of plant immune inducer and its application
CN112159283A (en) * 2020-10-18 2021-01-01 山东喜美农业科技有限公司 Preparation and use methods of chitosan oligosaccharide coated slow-release fertilizer
CN112175143A (en) * 2020-10-18 2021-01-05 山东喜美农业科技有限公司 Preparation method of chitosan oligosaccharide coated sustained release agent

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CN105132344A (en) * 2015-10-13 2015-12-09 黑龙江中农欣欣农业科技发展有限公司 Herbicide phytotoxicity biological antidote and preparing method and application thereof
CN106187498A (en) * 2016-07-17 2016-12-07 熊廷珍 Strengthen fertilizer and the production technology thereof of disease resistance of plant
CN107652041A (en) * 2017-10-16 2018-02-02 王欣 A kind of production method of the strong microbial manure of increasing both production and income, disease-resistant growth-promoting
CN108118000A (en) * 2017-11-29 2018-06-05 山东神农氏生物技术有限公司 A kind of high concentration disease-resistant compound microbial bacterial manure and production method

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CN101676242A (en) * 2008-09-17 2010-03-24 山东福田药业有限公司 Manufacturing method for producing anti-disease microbial fertilizer by utilizing corncob waste residue
CN102942420A (en) * 2012-11-30 2013-02-27 山东省林业科学研究院 Special microbial agent and bio-organic fertilizer for sweet cherry and preparation methods of special microbial agent
CN105036974A (en) * 2015-08-07 2015-11-11 沃达农业科技股份有限公司 Amino acid microbial ecological fertilizer and preparation and using method thereof
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Publication number Priority date Publication date Assignee Title
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CN112159283A (en) * 2020-10-18 2021-01-01 山东喜美农业科技有限公司 Preparation and use methods of chitosan oligosaccharide coated slow-release fertilizer
CN112175143A (en) * 2020-10-18 2021-01-05 山东喜美农业科技有限公司 Preparation method of chitosan oligosaccharide coated sustained release agent
CN112175143B (en) * 2020-10-18 2022-03-29 山东喜美农业科技有限公司 Preparation method of chitosan oligosaccharide coated sustained release agent
CN112159283B (en) * 2020-10-18 2022-05-13 史丹利化肥(平原)有限公司 Preparation and use methods of chitosan oligosaccharide coated slow-release fertilizer

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Application publication date: 20190226