CN109381411A - A kind of composition of anti-aging, preparation method and the cosmetics containing the composition - Google Patents
A kind of composition of anti-aging, preparation method and the cosmetics containing the composition Download PDFInfo
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Abstract
The invention belongs to cosmetic technical fields, disclose composition, preparation method and the cosmetics containing the composition of a kind of anti-aging.The composition of the anti-aging is Chinese medicine composition enzymolysis product, the Chinese medicine composition includes Radix Ophiopogonis, Fructus Corni, Radix Angelicae Sinensis and Wilson Begonia Rhizome, the Radix Ophiopogonis, Fructus Corni, Radix Angelicae Sinensis and Wilson Begonia Rhizome weight ratio be (4~8): (3~5): (0.5~1.5): (2~4).The advantage of the invention is that, it extracts using the Radix Ophiopogonis of special ratios, Fructus Corni, Radix Angelicae Sinensis and Wilson Begonia Rhizome mixing for the first time and obtains its enzymolysis product, amino acid and active peptide rich in the enzymolysis product, wherein active peptide has stronger resistance to oxidation, amino acid abundant can provide nutrition, and can improve the permeability of anti-apolexis composition, supplement moisture content, it is multi-faceted jointly to anti-aging, anti-aging effects are significant.
Description
Technical field
The invention belongs to cosmetic technical fields, and in particular to a kind of composition of anti-aging, preparation method and containing should
The cosmetics of composition.
Background technique
The skin aging of most people was begun to from 25 years old substantially, mainly the naturally-aged of gene control and many outer
Aging caused by the sexual factor of source.Currently, mainly having following measures to anti-aging: antiultraviolet, nutritional supplementation, resists moisturizing moisturizing
Microcirculation disorder, enhancing cell viability are eliminated in oxidation, anti-glycosylation, collagen synthesis and protection.Nowhere not due to ultraviolet light
Ultraviolet light can not be contacted completely in, people, therefore, for antiultraviolet outside other measures attention it is particularly important.
Moisture provides solvent medium for nutritional ingredient supply and metabolic waste exclusion, is divided between cell, greatly in skin
Lubricant and interleaving agent between son, water shortage directly cause dysbolism, and active force enhances between intermolecular, cell, skin
Skin is hardened, and contact probability increases between macromolecular, and cross-linking reaction is easier to occur between macromolecular, and sclerosis of the skin cannot reverse；Desmosome
Not exclusively, horn cell residual adhesion forms the scales of skin that peel off in skin surface for hydrolysis.Therefore, skin contains moisture appropriate and facilitates
It delays senescence and keeps smooth, facilitate to alleviate skin aging by moisturizing moisturizing.
The seven big nutritional ingredients that skin needs are mainly: amino acid and peptide, fatty acid, sugar, vitamin, micro and constant are first
Element, water, oxygen.Under normal circumstances, the circulatory system can carry out sufficient nutrition to skin tape, but with the aging and aging of human body
Bring sickness influence, causes microcirculation dysfunction, affects the supply or absorption of nutritional ingredient, and skin is once declining
Always, then more nutriments also can not preferably absorb, and can preferably absorb the nutrition in cosmetics is that cosmetics keep skin
The key of skin youth.
It is anti-oxidant in human body, the active oxygen of excessive free radicals and free radical mediated is mainly removed, human body has had itself
Antioxidant system contains numerous antioxidases and oxidation-resistant active ingredient in this system.Antioxidase is mainly: super oxygen
Object mutase, catalase, Selenium enrichment, selenium-independent-GPX and glutathione sulphur
Transferase, aldehyde ketone reductase；Antioxidant content is mainly: vitamin C, vitamin E, Co-Q10, glutathione, carotenoids
Element, bilirubin and some albumen.With the aging of human body, human body antioxidant system can also fail, and be mainly shown as antioxidase
Oxidation resistance caused by reducing with antioxidant content reduces.
As for anti-glycosylation, collagen synthesis and protection, elimination microcirculation disorder and enhancing cell viability, these are anti-
Aging measure by moisturizing moisturizing, nutritional supplementation and anti-oxidant can obtain biggish improvement.Therefore, moisturizing moisturizing, nutrition
Supplement and it is anti-oxidant be the key that research and development anti-aging cosmetics.However, current anti-aging cosmetics are there are biggish defect,
The good mostly hormone of using effect, and it is not added with hormone cosmetics, it is contemplated that the limitation of addition cost or preparation process, mostly
Only addition improves the antioxidative active constituent of cosmetics, and consumer's use feeling is poor, and Market Feedback is poor.Therefore, existingization
The even some substances for not allowing to add of some hormones or hormone analogs are added in the more or less meeting of cosmetic, to make consumer
Significant anti-aging effects, additional income can be seen in a short time.
With the continuous progress of research, it has been found that rice active polypeptide, tea seed active peptides and sweet potato active peptides
Etc. vegetalitas polypeptide to health of people etc. have biggish benefit, these products can be applicable to health care product, food or
In cosmetics.It is no lack of the medicinal material for having larger benefit to body in Chinese medicine, however, current research is also only limitted to independent plant
The research of active peptide, and common food mostly in people's daily life, the research for the active peptide of Chinese medicine ancient prescription or medicinal material
It has not been reported.If can be developed using Chinese medicine it is a kind of can anti-oxidant extra-nutrition and moisture again cosmetics, in many ways
Aging is alleviated in position, reduces addition cost, simplifies preparation process, then can overcome in order to which effect obviously adds hormone or hormone analogs
Defect.
Summary of the invention
In order to solve the above problems existing in the present technology, it is an object of that present invention to provide one kind can it is anti-oxidant but also
The anti-apolexis composition to supplement the nutrients with moisture resists the present invention also provides the preparation method of anti-apolexis composition and containing this
The cosmetics of anti-aging composition.
In Chinese medicine, Radix Ophiopogonis, Fructus Corni, Radix Angelicae Sinensis and Wilson Begonia Rhizome are had the property that
Radix Ophiopogonis: sweet, slight bitter, it is slightly cold.Moistening lung and clearing away the heart-fire purges heat and promotes the production of body fluid, resolving sputum preventing or arresting vomiting, controls row water of coughing；
Fructus Corni: nature and flavor acid, puckery, tepor.Return liver, kidney.It tonifies the liver and kidney, arresting collape syndrome by astringents.For dizziness and tinnitus, waist and knee acid
Bitterly, impotence and seminal emission, enuresis frequent micturition, metrorrhagia and metrostaxis leukorrhagia, profuse sweating collapse, interior heat disappear；
Radix Angelicae Sinensis: sweet, acrid, warm.Return liver, the heart, the spleen channel.Replenishing and activating blood, regulating menstruation and relieving pain, moistening intestines and relaxing bowels.It is dizzy for blood deficiency chlorosis
Dizzy palpitaition, irregular menstruation, amenorrhea and algomenorrhea, asthenia cold abdominalgia, rheumatic arthralgia, injury from falling down, ulcer sores, dry constipation of intestines.Prepared RADIX ANGELICAE SINENSIS with yellow rice wine
Blood circulation.For amenorrhea and algomenorrhea, rheumatic arthralgia, injury from falling down；
Wilson Begonia Rhizome: cold nature, it is sweet in flavor, it is bitter.Return kidney channel, Liver Channel.Benefiting qi and nourishing blood, dissipating stasis and stanching bleeding.Classify under category hemostatic
Stasis-resolving hemostatic.
The technical scheme adopted by the invention is as follows:
The present invention provides a kind of composition of anti-aging, the composition be Chinese medicine composition enzymolysis product, it is described in
Drug composition includes Radix Ophiopogonis, Fructus Corni, Radix Angelicae Sinensis and Wilson Begonia Rhizome, the Radix Ophiopogonis, Fructus Corni, Radix Angelicae Sinensis and Wilson Begonia Rhizome weight ratio be (4
~8): (3~5): (0.5~1.5): (2~4).
Preferably, the composition of above-mentioned anti-aging, the Radix Ophiopogonis, Fructus Corni, Radix Angelicae Sinensis and Wilson Begonia Rhizome weight ratio be 6:4:
1:3。
The present invention also provides a kind of preparation methods of anti-apolexis composition above-mentioned, include the following steps:
(1) it crushes: weighing Radix Ophiopogonis, Fructus Corni, Radix Angelicae Sinensis and Wilson Begonia Rhizome according to quantity, pulverize and sieve, obtain Chinese medicine composition powder；
(2) it dissolves: the Chinese medicine composition powder is added to the water, dissolve, ultrasonic treatment obtains feed liquid；
(3) add alkali: NaOH solution being added in Xiang Suoshu feed liquid, supernatant is collected by centrifugation in stirring and leaching, obtains Chinese medicine albumen and mentions
Take liquid；
(4) acid adding: being added HCl solution in Xiang Suoshu Chinese medicine protein extract, centrifuging and taking precipitates after reaction, dry, obtains
Chinese medicine crude protein dry product；
(5) multistage enzymatic hydrolysis: the Chinese medicine crude protein dry product is dissolved, obtains Chinese medicine crude protein solution, Xiang Suoshu Chinese medicine is thick
Pepsin is added in protein solution, carries out primary enzymolysis, obtains reaction solution one, tryptose is added in Xiang Suoshu reaction solution one
Enzyme carries out secondary enzymolysis, obtains reaction solution two, papain is added in Xiang Suoshu reaction solution two, is digested three times, obtains
Reaction solution three will dry after three enzyme deactivation of reaction solution, obtain Chinese medicine composition active peptide；The pepsin, trypsase
Ratio with the mole of papain is 5:3:2.
Preferably, the preparation method of above-mentioned anti-apolexis composition, Chinese medicine composition powder quality in the step (2) with
The ratio of the volume of water is 1:9-1:11.
Preferably, the preparation method of above-mentioned anti-apolexis composition, in the step (3) in NaOH solution solute mass body
Product percentage is 20%, and the temperature in the step (3) when stirring and leaching is 50~55 DEG C, stirring and leaching in the step (3)
When pH be 8.5~9, the time of stirring and leaching is 4~5h in the step (3).
Preferably, the preparation method of above-mentioned anti-apolexis composition, the concentration of HCl solution is 1mol/L in the step (4),
The pH of step (4) reaction is 4.5~5.
Preferably, the preparation method of above-mentioned anti-apolexis composition, primary enzymolysis, secondary enzymolysis and three in the step (5)
The temperature of secondary enzymatic hydrolysis is respectively 37 DEG C, 42 DEG C and 55 DEG C, primary enzymolysis in the step (5), secondary enzymolysis and is digested three times
Time is respectively 5h, 5h and 2h, and primary enzymolysis, secondary enzymolysis and the pH digested three times are respectively 2,8 and in the step (5)
6.5。
The present invention also provides a kind of anti-aging cosmetics, the anti-aging cosmetics include anti-aging combination above-mentioned
Object, the anti-aging cosmetics are any one in antisenility skin care water, anti-aging lotion or anti-aging creams.
Specifically, the anti-aging cosmetics are mainly by following when the anti-aging cosmetics are antisenility skin care water
The raw material of weight percent is prepared: anti-apolexis composition 8~12%, polyethylene glycol -4 5~8%, 2~5% and of butanediol
Preservative 0.1~0.4%, surplus are deionized water.
Specifically, the anti-aging cosmetics are mainly by following heavy when the anti-aging cosmetics are anti-aging lotion
The raw material of amount percentage is prepared: glycerol 2~4%, apricot kernel oil 4~6%, saualane 3~7%, emulsifier 3~6%, tristearin
Alcohol 2~4%, Gantrez AN-119 0.2~0.4%, preservative 0.2~3% and anti-apolexis composition above-mentioned 10~16%, it is remaining
Amount is deionized water；The emulsifier is the mixed of one or both of octadecyltrimethylammonium chloride and oleth -10
Close object.
The invention has the benefit that
Anti-apolexis composition provided by the invention, it is mixed using the Radix Ophiopogonis of special ratios, Fructus Corni, Radix Angelicae Sinensis and Wilson Begonia Rhizome for the first time
It closes to extract and obtains its enzymolysis product, since the amino acid content of Radix Ophiopogonis, Fructus Corni is very high, amino acid abundant can be obtained after hydrolysis
And active peptide, while mixed hydrolysis Radix Angelicae Sinensis and Wilson Begonia Rhizome, so that amino acid i.e. rich in the enzymolysis product finally contained
And active peptide, and contain temperature compensation active constituent, wherein active peptide has stronger resistance to oxidation, alleviates human body antioxidant system
Microgroove caused by failing, obscure etc., amino acid abundant can provide nutrition, and can improve the permeability of anti-apolexis composition,
Moisture content is supplemented, skin of face microcirculation is adjusted in temperature compensation active constituent, promotes the absorption of amino acid and active peptide, multi-faceted common
To anti-aging, anti-aging effects are significant.
Meanwhile during the extraction process, using the heavy condition of specific alkali carries acid, protein yield is higher, using different enzymes
Multistage enzymatic hydrolysis is carried out, the protein being fully hydrolyzed in Radix Ophiopogonis, Fructus Corni, Radix Angelicae Sinensis and Wilson Begonia Rhizome sufficiently exposes active peptide therein
Restriction enzyme site, multiple spot cutting so that Asp, Glu, Phe and Lys for containing in enzymolysis product are more, Asp, Glu, Phe and Lys
Content is high, and the inoxidizability of active peptide is stronger.Secondly, it is suitable to be equipped with by using anti-apolexis composition provided by the invention
Moisturizer, emulsifier etc., the anti-aging cosmetics made have excellent inoxidizability, permeability, it is full of nutrition, resist
Aging effects are more preferable.
Detailed description of the invention
Fig. 1 is the albumen yield of anti-apolexis composition of the invention at different alkali carries pH；
Fig. 2 is the albumen yield of anti-apolexis composition of the invention at a temperature of different alkali carries；
Fig. 3 is the albumen yield of anti-apolexis composition of the invention at the heavy pH of different acid；
Fig. 4 is the albumen yield of anti-apolexis composition of the invention in different feed liquid ratio；
Fig. 5 is the amino acid accounting of anti-apolexis composition of the invention in the enzymolysis product under different enzymatic hydrolysis conditions；
Fig. 6 is the reducing power measurement of anti-apolexis composition of the invention；
Fig. 7 is the DDPH clearance rate of anti-apolexis composition of the invention；
Fig. 8 is the ultra-oxygen anion free radical clearance rate of anti-apolexis composition of the invention；
Fig. 9 is the hydroxyl radical free radical clearance rate of anti-apolexis composition of the invention；
Figure 10 is the resistance to oxidation measurement result of the anti-apolexis composition of different proportion of the invention；
Figure 11 is the resistance to oxidation measurement result of anti-aging cosmetics of the invention.
Specific embodiment
Test example does further explaination to the present invention with reference to the accompanying drawing and specifically.
Test example 1:
This test example is designed to provide a kind of anti-apolexis composition, and preparation method is as follows:
1. pulverizing and sieving
Weigh Chinese medicine composition: Radix Ophiopogonis, Fructus Corni, Radix Angelicae Sinensis and Wilson Begonia Rhizome.Chinese medicine composition is passed through into LABORATORY
After 3100 crusher of MILL crushes, 120-200 mesh is crossed, Chinese medicine composition powder is obtained.
2. alkali extraction and acid precipitation extracts Chinese medicine crude protein
The Chinese medicine composition powder for weighing 100g is made it dissolve under the conditions of certain solid-liquid ratio with ultrapure water, sufficiently molten
Xie Hou adjusts its alkali carries pH using 20%NaOH (w/v) solution, controls alkali carries temperature, and 4~5h of stirring and leaching obtains leaching liquor,
By leaching liquor with 5000r/min, the condition of 15min is centrifuged, and collects supernatant, as Chinese medicine protein extract.
The heavy pH of acid for adjusting Chinese medicine composition powder with 1mol/L HCl solution, after the appearance of a large amount of sediments, with
The condition of 5000r/min, 15min are centrifuged, and are abandoned liquid, are stayed precipitating, as Chinese medicine crude protein, gained Chinese medicine crude protein are used super
It is neutrality that pure water, which is washed repeatedly to its cleaning solution, dry with vacuum freeze drier, obtains Chinese medicine crude protein dry product, is placed in 4
It is saved in DEG C refrigerator.Design parameter see the table below:
Table 1
| Alkali carries pH | Alkali carries temperature (DEG C) | The heavy pH of acid | Solid-liquid ratio |
| 7.5 | 35 | 3.5 | 1:5 |
| 8. | 40 | 4 | 1:7 |
| 8.5 | 45 | 4.5 | 1:9 |
| 9 | 50 | 5 | 1:11 |
| 10 | 55 | 5.5 | 1:13 |
| 60 |
Protein content in Chinese medicine crude protein dry product, protein content and Chinese medicine in Chinese medicine crude protein dry product are measured using BCA method
The ratio of protein content is its recovery rate in composition powder.Recovery rate result under each parameter is as shown in Figs 1-4.By Fig. 1
It is found that being continuously increased with alkali carries pH, protein must take the lead in reducing after increasing, pH be more than after 9 protein yield be remarkably decreased,
Under the conditions of pH is 8.5, protein yield highest.As shown in Figure 2, being continuously increased with alkali carries temperature, protein must take the lead in
Reduced after raising, temperature be more than after 55 DEG C protein yield be remarkably decreased, under the conditions of temperature is 50 DEG C, protein yield is most
It is high.From the figure 3, it may be seen that being continuously increased with the heavy pH of acid, protein must take the lead in reducing after increasing, become pH be more than after 5 protein obtain
Rate is remarkably decreased, under the conditions of pH is 4.5, protein yield highest.As shown in Figure 4, solid-liquid ratio is in 1:9~1:11, to albumen
Matter yield amplitude is little, but solid-liquid ratio protein yield highest in 1:11.
It can be seen that Radix Ophiopogonis, Fructus Corni, Radix Angelicae Sinensis and Wilson Begonia Rhizome composition optimal extraction conditions are as follows: alkali carries pH 8.5,
Alkali carries temperature 50 C, the heavy pH of acid is 4.5, solid-liquid ratio 1:11.
3. the preparation of Chinese medicine composition enzymolysis product
Above-mentioned Chinese medicine crude protein dry product is formulated as Chinese medicine crude protein solution, stomach is added in Xiang Suoshu Chinese medicine crude protein solution
Protease obtains reaction solution one, trypsase is added in Xiang Suoshu reaction solution one in 37 DEG C or so progress primary enzymolysis, carries out
Secondary enzymolysis obtains reaction solution two, papain is added in Xiang Suoshu reaction solution two, is digested three times, reaction solution is obtained
Three, it will be dried after the three water-bath enzyme deactivation of reaction solution, obtain Chinese medicine composition enzymolysis product dry product；The trypsase, stomach egg
The ratio of the mole of white enzyme and papain is 5:3:2, and the mass ratio of single enzyme and Chinese medicine crude protein dry product is 4:100.
Chinese medicine crude protein dry product is formulated as Chinese medicine crude protein solution, first Chinese medicine crude protein solution is carried out in advance with ultrasonic wave
Processing avoids albumen adhesion, then Chinese medicine crude protein solution water-bath is heated to hydrolysis temperature appropriate, enzyme needed for reaction is added
Amount, stirring, insulation reaction for a period of time, obtain enzymolysis liquid.During the reaction with 0.2mol/L NaOH or 0.5mol/L HCL
Maintain pH value constant.Enzymolysis liquid is placed in enzyme deactivation in boiling water bath after reaction, enzymolysis liquid value is adjusted to 4.4 after cooling, and
It is centrifuged 5min under the conditions of 4000r/min, removes supernatant, gained supernatant tune pH to 7.0, vacuum concentration is after freeze-drying
Chinese medicine composition enzymolysis product dry product.Complex enzyme are as follows: pepsin, trypsase and papain.Complex enzyme zymohydrolysis includes
Stepwise discretization three times, primary enzymolysis, secondary enzymolysis and the temperature digested three times are respectively 37 DEG C, 40 DEG C and 56 DEG C, primary enzymolysis,
Secondary enzymolysis and the time digested three times are respectively 5h, 5h and 2h, and primary enzymolysis, secondary enzymolysis and the pH digested three times are respectively
2,8 and 6.5.
Since pepsin, trypsase and papain individually digest above-mentioned composition, cleavage site is single, hydrolysis
Obtained polypeptide wants content low, and different surely hydrolysis, which is arrived, contains antioxidative active peptide, therefore inoxidizability is inadequate.Different
Test objective can also take enzymatic hydrolysis using different enzymes, therefore general at present using single enzyme enzymatic hydrolysis.Sublevel is carried out using different enzymes
Section enzymatic hydrolysis can obtain the peptide of more classifications, but due to the reactive conditions of various enzymes difference, and different plants needs difference
Enzyme go to digest, table 2 show complex enzyme optimal conditions, it is as follows:
Table 2
| Grouping | Classification | Temperature (DEG C) | pH | Time (h) |
| A | Trypsase | 40 | 8 | 10 |
| B | Pepsin | 37 | 2 | 10 |
| C | Papain | 56 | 6.5 | 10 |
| D | Complex enzyme | 37→40→56 | 2→8→6.5 | 5→5→2 |
4. the measurement of amino acid in enzymolysis product dry product
20-30mg Chinese medicine composition enzymolysis product dry product (difference group enzyme enzymolysis product, amount to four groups) is accurately weighed, is added
10mL 6mol/L HCl, leads to high pure nitrogen 2min, and sealing is put in 110 DEG C of baking ovens and hydrolyzes 22h, after the completion of hydrolysis, taking-up is put
It sets to room temperature, filtering takes 1mL filtrate to be placed in a beaker, and in 60 DEG C of water bath methods, 1mL ultrapure water is then added, continues to be evaporated,
1mL ultrapure water is added again, after being evaporated, is put in be measured in 4 DEG C of refrigerators.When measuring sample, machine on 4mL is added into each beaker
Solvent takes out 1.5mL sample with abundant sample dissolution, 0.22 μm of membrane filtration, mixes solvent on 1mL, and upper machine measurement is owned
Measurement is completed in 2 days.Sampling volume 50 μ L, column model LCA K06/Na, mobile phase sodium citrate A=0.12N, pH
3.45；B=0.2N, pH 10.5.Elution flow rate pump is set as 0.4mL/min, and derivative flow rate pump is set as 0.25mL/min.Temperature
Degree gradient is set as 60-75 DEG C, and pressure controls between 30-40bar.Since the maximum absorption wavelength of proline is 440nm, and its
The maximum absorption wavelength of its amino acid is 570nm, therefore, while acquiring the signal under two wavelength of 570nm and 440nm, respectively
To its accurate quantitative analysis.
A, B, C and D respectively represent the amino acid composition for the enzymolysis product being respectively grouped in table 2, as shown in Figure 5, D group in Fig. 5
Asp, Glu, Phe and Lys content are significantly larger than other three groups in complex enzyme zymohydrolysis product, show that this group of enzymolysis product has more
The active peptide of amino acid abundant and oxidation resistance.The Combined Treatment of pepsin and trypsase, so that in enzymolysis product
Protein is cut in different loci, to obtain active peptide more containing Asp, Glu, Phe and Lys, while again with without special
Property cutting papain cut 2h, amino acid more abundant can be obtained.
5. enzymolysis product dry product antioxidant activity is evaluated
The measurement of 5.1 DPPH free radical scavenging activities
150 μm of ol/L DPPH stock solutions are prepared with 80% ethyl alcohol, before test, are diluted to required concentration with 80% ethyl alcohol.It adopts
With the DPPH free radical scavenging activity of 96 orifice plates measurement sample.20 μ L ultrapure waters and 180 μ L DPPH solution are added, carry out all-wave
Section scanning, a length of 522nm of maximum absorption wave (microplate reader program: oscillation 30s measures wave-length coverage 300-700nm).Test sample
When with the free radical scavenging activity of positive control Vc, regulation system absorbance value to 0.70 ± 0.02 obtains working solution.Sample
Measurement: setting wavelength as 522nm, and tetra- groups of enzymolysis products of A, B, C and D of 20 μ L various concentrations are added, and concentration unit is μ g/mL.
The measurement of 5.2 ultra-oxygen anion free radical Scavenging activities
Autoxidation occurs under the conditions of weak base for pyrogallol, generates O2。·O2Scavenger can be such that mouse thymus cells produce
Absorption peak of the object at 325nm weakens, and by the change rate of light absorption value A325, calculates to O2Scavenging activity.Take 4.5mL's
50mmol/L phosphate buffer and 4.2mL distilled water keep the temperature 20min in 25 DEG C of water-baths after mixing, are added immediately after taking-up
0.2mmol/L the pyrogallol 0.3mL, total volume 9mL of 25 DEG C of preheatings；After shaking up rapidly, measures inhale every 30s at 325 nm
Light value calculates the value added of absorbance per minute in the linear range.Sample is that tetra- groups of enzymatic hydrolysis of A, B, C and D of various concentration produce
Object, concentration unit are μ g/mL.It is the same to the taken reagent of test sample in addition to replacing distilled water with sample liquid, it calculates according to the above method
The value added of absorbance per minute in the range of linearity.The ratio of the difference of the difference and blank absorbency of blank and sample absorbance
As O2 clearance rate.
The measurement of 5.3 hydroxyl radical free radical Scavenging activities
6mmol/L FeSO is sequentially added in 20mL colorimetric cylinder42mL, 6mmol/L salicylic acid 2mL, test sample antioxygen
Change peptide aqueous solution 2mL, 6mmol/L H is added after shaking up2O22mL starting reaction, adds distilled water to be settled to 8mL, shakes up at room temperature
Stand 30min.Measurement is not added the absorbance A s of bigcatkin willow acid system, the absorbance A y for being loaded product system, sample liquid system is not added
Absorbance A0, sample may be expressed as: the Scavenging activity of OH
M is clearance rate of each sample to OH.
The measurement of 5.4 reducing powers
Substance with stronger reducing power can be Fe3+It is reduced into Fe3+, the degree of reduction is judged by chromogenic reaction, instead
Absorbance is bigger after answering, and illustrates that the reducing power of the substance is stronger.Concrete operations: add in 6.6 phosphate buffer of 2.5mL pH
Enter test sample, 1% potassium ferricyanide 1mL, by mixed liquor in 50 DEG C of constant temperature 20min, adds three chloroethenes that 1mL concentration is 10%
Acid, then 3000r/min is centrifugated 10min, and supernatant liquor is taken to add distilled water 2.5mL and 0.1%FeCl3, it is uniformly mixed, it is quiet
After setting 10min, light absorption value at 700nm is measured, all measured values are average value three times.Light absorption value is bigger, indicates reducing power
It is stronger.
Antioxidant activity result is as shown in figs. 7-9.In Fig. 7~9, A, B, C and D respectively represent the enzymatic hydrolysis being respectively grouped in table 2
The clearance rate of the DPPH free radical of product, ultra-oxygen anion free radical and hydroxyl radical free radical, under various concentration, the DPPH of D group
Free radical, ultra-oxygen anion free radical and hydroxyl radical free radical clearance rate are above other groups；Meanwhile it will be appreciated from fig. 6 that A, B, C and D
The reducing power measurement for respectively representing the enzymolysis product being respectively grouped in table 2, with the raising of enzymolysis product concentration, reducing power also by
Edge up height, but the reducing power of D group is apparently higher than other three groups, and it is preferable to show that the product after being digested by several times using complex enzyme is had
Inoxidizability.
Test example 2
Based on Radix Ophiopogonis, Fructus Corni, Radix Angelicae Sinensis and the existing drug effect of Wilson Begonia Rhizome, Radix Ophiopogonis, Fructus Corni, Radix Angelicae Sinensis and Wilson Begonia Rhizome are individually led to
Method provided by overtesting example 1 is verified, although containing certain amino acid after finding individual Radix Ophiopogonis or fruit of medicinal cornel enzymatic hydrolysis,
With certain oxidation resistance, but its oxidation resistance is not so good as plus the oxidation resistance after Radix Angelicae Sinensis and Wilson Begonia Rhizome, and Radix Angelicae Sinensis
Or then amino acid content is lower for the product that individually digests of Wilson Begonia Rhizome, oxidation resistance is relatively low.Therefore, it is mixed into using four traditional Chinese medicine
Row enzymatic hydrolysis.
Weigh Chinese medicine composition: three groups are respectively set in Radix Ophiopogonis, Fructus Corni, Radix Angelicae Sinensis and Wilson Begonia Rhizome, specific as follows:
Ratio 1: Radix Ophiopogonis: Fructus Corni: Radix Angelicae Sinensis: the mass ratio of Wilson Begonia Rhizome is 4:3:1.5:4；
Ratio 2: Radix Ophiopogonis: Fructus Corni: Radix Angelicae Sinensis: the mass ratio of Wilson Begonia Rhizome is 8:5:1.5:4；
Ratio 3: Radix Ophiopogonis, Fructus Corni, Radix Angelicae Sinensis and Wilson Begonia Rhizome weight ratio be 6:4:1:3；
The other composition of above three group is slightly mentioned and digested according to method shown in test example 1, and verifies three groups not
With the inoxidizability of the enzymolysis product of composition, the results are shown in Figure 10, as shown in Figure 10, suitable proportion, Fructus Corni, Radix Angelicae Sinensis
And Wilson Begonia Rhizome, the resistance to oxidation of the final enzymolysis product of composition are higher than Radix Ophiopogonis and the excessively high group (ratio 2) of Fructus Corni and work as
Return, the group (ratio 1) that Wilson Begonia Rhizome accounting is excessively high, optimal ratio is 6:4:1:3.
Test example 3
This test example is designed to provide a kind of anti-aging cosmetics.
Antisenility skin care water is mainly prepared by the raw material of following parts by weight: (the ratio 3 of anti-apolexis composition 8~12%
D group enzymolysis product), polyethylene glycol -4 5~8%, butanediol 2~5% and preservative 0.1~0.4%, surplus be deionization
Water.
When anti-aging lotion, mainly it is prepared by the raw material of following weight percent: glycerol 2~4%, apricot kernel oil 4~
6%, saualane 3~7%, emulsifier 3~6%, stearyl alcohol 2~4%, Gantrez AN-119 0.2~0.4%, preservative 0.2~
3% and test example 1 anti-apolexis composition 10~16% (the D group enzymolysis product of ratio 3), surplus is deionized water；The cream
Agent is the mixture of one or both of octadecyltrimethylammonium chloride and oleth -10.
The free radical scavenging activity of anti-aging skin care product (only shows that antisenility skin care water number evidence is not shown) as shown in figure 11.By
It is found that as anti-apolexis composition adds the increase of concentration, the clearance rate of each free radical gradually increases Figure 10.10~16%
(w/w) under addition concentration, free radical scavenging activity can reach 60% or more, it can be seen that, the anti-aging combination under the concentration
Object effect is optimal.
The present invention is not limited to above-mentioned optional embodiment, anyone can show that other are each under the inspiration of the present invention
The product of kind form.Above-mentioned specific embodiment should not be understood the limitation of pairs of protection scope of the present invention, protection of the invention
Range should be subject to be defined in claims, and specification can be used for interpreting the claims.
Claims (10)
1. a kind of composition of anti-aging, it is characterised in that: the composition is Chinese medicine composition enzymolysis product, the Chinese medicine group
Closing object includes Radix Ophiopogonis, Fructus Corni, Radix Angelicae Sinensis and Wilson Begonia Rhizome, the Radix Ophiopogonis, Fructus Corni, Radix Angelicae Sinensis and Wilson Begonia Rhizome weight ratio be (4~
8): (3~5): (0.5~1.5): (2~4).
2. the composition of anti-aging according to claim 1, it is characterised in that: the Radix Ophiopogonis, Fructus Corni, Radix Angelicae Sinensis and a bit
The weight ratio of blood is 6:4:1:3.
3. a kind of preparation method of anti-apolexis composition of any of claims 1 or 2, which comprises the steps of:
(1) it crushes: weighing Radix Ophiopogonis, Fructus Corni, Radix Angelicae Sinensis and Wilson Begonia Rhizome according to quantity, pulverize and sieve, obtain Chinese medicine composition powder；
(2) it dissolves: the Chinese medicine composition powder is added to the water, dissolve, ultrasonic treatment obtains feed liquid；
(3) add alkali: NaOH solution being added in Xiang Suoshu feed liquid, supernatant is collected by centrifugation in stirring and leaching, obtains Chinese medicine protein extraction
Liquid；
(4) acid adding: being added HCl solution in Xiang Suoshu Chinese medicine protein extract, centrifuging and taking precipitates after reaction, dry, obtains Chinese medicine
Crude protein dry product；
(5) multistage enzymatic hydrolysis: the Chinese medicine crude protein dry product is dissolved, Chinese medicine crude protein solution, Xiang Suoshu Chinese medicine crude protein are obtained
Pepsin is added in solution, carries out primary enzymolysis, obtains reaction solution one, trypsase is added in Xiang Suoshu reaction solution one, into
Row secondary enzymolysis obtains reaction solution two, papain is added in Xiang Suoshu reaction solution two, is digested three times, is reacted
Liquid three will dry after three enzyme deactivation of reaction solution, obtain Chinese medicine composition active peptide；The pepsin, trypsase and wood
The ratio of the mole of melon protease is 5:3:2.
4. the preparation method of anti-apolexis composition according to claim 3, it is characterised in that: in the step (2)
The ratio of the volume of drug composition powder quality and water is 1:9-1:11.
5. the preparation method of anti-apolexis composition according to claim 4, it is characterised in that: NaOH in the step (3)
The quality percentage by volume of solute is 20% in solution, and the temperature in the step (3) when stirring and leaching is 50~55 DEG C, described
PH in step (3) when stirring and leaching is 8.5~9, and the time of stirring and leaching is 4~5h in the step (3).
6. the preparation method of anti-apolexis composition according to claim 5, it is characterised in that: HCl is molten in the step (4)
The concentration of liquid is 1mol/L, and the pH of step (4) reaction is 4.5~5.
7. the preparation method of anti-apolexis composition according to claim 6, it is characterised in that: primary in the step (5)
Enzymatic hydrolysis, secondary enzymolysis and the temperature digested three times are respectively 37 DEG C, 42 DEG C and 55 DEG C, primary enzymolysis in the step (5), secondary
Enzymatic hydrolysis and time for digesting three times are respectively 5h, 5h and 2h, primary enzymolysis, secondary enzymolysis and are digested three times in the step (5)
PH be respectively 2,8 and 6.5.
8. a kind of anti-aging cosmetics, it is characterised in that: the anti-aging cosmetics include of any of claims 1 or 2 anti-ageing
Old composition, the anti-aging cosmetics are any one in antisenility skin care water, anti-aging lotion or anti-aging creams.
9. anti-aging cosmetics according to claim 8, which is characterized in that when the anti-aging cosmetics are anti-aging shield
When skin water, the anti-aging cosmetics are mainly prepared by the raw material of following weight percent: anti-apolexis composition 8~
12%, polyethylene glycol -4 5~8%, butanediol 2~5% and preservative 0.1~0.4%, surplus are deionized water.
10. anti-aging cosmetics according to claim 8, which is characterized in that when the anti-aging cosmetics are anti-aging
When lotion, the anti-aging cosmetics are mainly prepared by the raw material of following weight percent: glycerol 2~4%, apricot kernel oil 4
~6%, saualane 3~7%, emulsifier 3~6%, stearyl alcohol 2~4%, Gantrez AN-119 0.2~0.4%, preservative 0.2
~3% and anti-apolexis composition of any of claims 1 or 2 10~16%, surplus be deionized water；The emulsifier is 18
The mixture of one or both of alkyl trimethyl ammonium chloride and oleth -10.
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