CN109369266A - A kind of compound microorganism ferments fertilizer and preparation method thereof for planting fruit trees - Google Patents
A kind of compound microorganism ferments fertilizer and preparation method thereof for planting fruit trees Download PDFInfo
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- CN109369266A CN109369266A CN201811396409.4A CN201811396409A CN109369266A CN 109369266 A CN109369266 A CN 109369266A CN 201811396409 A CN201811396409 A CN 201811396409A CN 109369266 A CN109369266 A CN 109369266A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D1/00—Fertilisers containing potassium
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The compound microorganism ferments fertilizer and preparation method thereof that the invention discloses a kind of for planting fruit trees, the raw material including following parts by weight: 40-60 parts of black pig Metabolism Excretion object, 50-70 parts of seedling vine, 35-51 parts of sheep barnyard manure, 15-35 parts of wheat bran, 20-28 parts of rice straw, 38-46 parts of N P and K base fertilizer, 3-11 parts of trace-element fertilizer, 20-28 parts of trichoderma chlamydospore bacterium, 10-18 parts of Folithion missible oil, 13-21 parts of pantoea agglomerans, 15-27 parts of cellulolytic Mixed Microbes bacterium solution, 12-20 parts of fixing nitrogen, dissolving phosphor and dissolving potassium compound bacteria liquid.Compound microorganism ferments fertilizer prepared by the present invention improves the conversion ratio of fertilizer, reduces the loss of chemical fertilizer, improves the utilization rate of fertilizer.
Description
Technical field
The present invention relates to microbial fermentation fertilizer, specially a kind of compound microorganism ferments fertilizer for planting fruit trees and
Preparation method.
Background technique
Fruit tree is perennation plant, and fertilizer requirement is very big, all needs supplement nitrogen, phosphorus, potassium and a large amount of organic fertilizer every year, and
In order to guarantee that fruit should be properly added microelement, yield rate is improved.But it due to applying a large amount of inorganic nutrients every year, causes
The imbalance of nutrition, utilization rate is low, soil deterioration, and hardened serious, therefore, urgent problem, is reasonably to apply fertilizer at present, will
Superfluous nutrient uses in soil, reduces chemical fertilizer waste, is lost and pollutes.
The patent application of Publication No. CN104609930A discloses a kind of pear tree high yield dedicated fertilizer, composition
It is as follows: dry human excrement, dry duck excrement, rape cake, compound fertilizer, dry rabbit excrement, potassium dihydrogen phosphate fertilizer, calcareous fertilisers, carbendazim, plant protein powder,
DDVP, the spirit of fruit tree despeckle, soya bean stem meal, ichor of bearing fruit, vinasse powder.Dedicated fertilizer manure use prepared by the present invention with it is inorganic
The mixing of fertilizer meets the growth and development demand of pear tree, but the utilization rate of fertilizer of invention preparation is low, easily causes the stream of fertilizer
It loses.
The patent application of Publication No. CN103340055A discloses a kind of applying for edible mushroom waste mushroom substitution fruit tree fertilizer
Fertile method, comprising the following steps: excavation width and depth are 30-40 centimetres of fertilising slot at the tree crown drip of fruit tree；
Edible mushroom waste mushroom is added in above-mentioned fertilising slot；The fertilising slot for having the waste edible fungus bacterium material will be added to fill.The present invention
The fertilizing method for this edible mushroom waste mushroom substitution fruit tree fertilizer that embodiment provides, whole process is not in soil block
Phenomenon, the organic macromolecule that edible mushroom waste mushroom obtains after being degraded can be absorbed by root system of plant, and then enable to fruit tree
Growth well.But the present invention is used only edible mushroom and substitutes fruit tree fertilizer, cannot provide whole nutrition needed for fruit tree growth
Ingredient.
Summary of the invention
The compound microorganism ferments fertilizer and preparation method thereof that the purpose of the present invention is to provide a kind of for planting fruit trees,
To solve the problems mentioned in the above background technology.
A kind of compound microorganism ferments fertilizer for planting fruit trees, the raw material including following parts by weight: black pig metabolism row
Let out 40-60 parts of object, 50-70 parts of seedling vine, 35-51 parts of sheep barnyard manure, 15-35 parts of wheat bran, 20-28 parts of rice straw, N P and K basis
It is 38-46 parts of fertilizer, 3-11 parts of trace-element fertilizer, 20-28 parts of trichoderma chlamydospore bacterium, 10-18 parts of Folithion missible oil, agglomerating general
13-21 parts of bacterium, 15-27 parts of cellulolytic Mixed Microbes bacterium solution, 12-20 parts of fixing nitrogen, dissolving phosphor and dissolving potassium compound bacteria liquid.
Preferably, the N P and K base fertilizer includes nitrogenous fertilizer, phosphate fertilizer, potash fertilizer；Trace-element fertilizer include copper, iron, manganese,
Zinc, boron, molybdenum.
Preferably, the fixing nitrogen, dissolving phosphor and dissolving potassium compound bacteria liquid is the preparation method is as follows: by potassium solubilizing bacteria, phosphate solubilizing bacteria and withered grass bud
Spore bacillus is respectively placed in respective culture medium and carries out inclined-plane culture 3-5 days, and cultivation temperature is 30-38 DEG C, will after culture
Potassium solubilizing bacteria, phosphate solubilizing bacteria and bacillus subtilis carry out mixed bacteria liquid according to 3:2:5.
Preferably, the cellulolytic Mixed Microbes bacterium solution the preparation method is as follows: cellulolytic bacterium is set
Vibration culture 2-4 days, obtain cellulolytic bacterial solution in beef extract agar medium；By cellulolytic fungi
It is placed in potato sugar agar medium and carries out vibration culture 4-6 days, obtain cellulolytic fungi bacterium solution；By cellulose point
Proportionally 2:5 is mixed solution property bacterial solution with cellulolytic fungi bacterium solution, obtains cellulolytic Mixed Microbes
Bacterium solution.
Preferably, the trichoderma chlamydospore bacterium the preparation method is as follows: trichoderma is seeded in PDA culture medium, in temperature
Degree washes lower spore, is formulated as Trichoderma spore suspension to cultivate 4-6 days under conditions of 30-38 DEG C, by Trichoderma spore suspension into
Row is diluted to clump count to be inoculated in maize powder medium, cultivation temperature 25-33 after 2 × 10^6-4 × 10^6cfu/ml
DEG C, mixing speed 200-260r/min, incubation time is 6-8 days, obtains trichoderma chlamydospore bacterium.
A kind of method for preparing compound microorganism ferments fertilizer for planting fruit trees, comprising the following steps:
(1), the raw material of above-mentioned parts by weight is weighed；Black pig Metabolism Excretion object is separated by solid-liquid separation, solid carries out after collecting
It dries, temperature is controlled at 35-43 DEG C, until water content is 20-32%, is then carried out UV treatment 60-80 minutes, at ultraviolet light
Sterilization processing is carried out using ozone after reason, in sterilization process, every 10-18 minutes progress turning and throwing onces, sterilizes 50-70min altogether；
Black pig Metabolism Excretion object after sterilization is mixed with sheep barnyard manure, mixing speed 300-400r/min, mixing time
For 100-120min；
(2), by the culture medium after pantoea agglomerans access sterilizing, under conditions of temperature is 30-38 DEG C, keynote will be cultivated
To pH value be 7.0-7.4, vacuum culture 44-60 hours；By the pantoea agglomerans and trichoderma chlamydospore bacterium, cellulose point after culture
Solution property Mixed Microbes bacterium solution, the mixing of fixing nitrogen, dissolving phosphor and dissolving potassium compound bacteria liquid, stir 32-40 minutes under conditions of 30-34 DEG C, obtain
Mixed bacteria liquid；
(3), it is put into after mixing seedling vine, wheat bran, rice straw and carries out being crushed to 40-52 mesh in pulverizer, then
The mixture obtained with N P and K base fertilizer, trace-element fertilizer, Folithion missible oil and step (1) be mixed
It is even, obtain mixture A；
(4), mixture A is spread in fermentor, the thickness of every 10-14 centimeters of paving, uniformly sprinkling step (2) obtains mixed
Combined bacteria liquid is simultaneously compacted, and repaves the second layer, and so on, until being paved with entire fermentor, fermentor is sealed anaerobic fermentation,
Obtain the compound microorganism ferments fertilizer for planting fruit trees.
Preferably, comprising the following steps:
(1), the raw material of above-mentioned parts by weight is weighed；Black pig Metabolism Excretion object is separated by solid-liquid separation, solid carries out after collecting
It dries, temperature is controlled at 37-41 DEG C, until water content is 23-29%, is then carried out UV treatment 65-75 minutes, at ultraviolet light
Sterilization processing is carried out using ozone after reason, in sterilization process, every 12-16 minutes progress turning and throwing onces, sterilizes 55-65min altogether；
Black pig Metabolism Excretion object after sterilization is mixed with sheep barnyard manure, mixing speed 325-375r/min, mixing time
For 105-115min；
(2), by the culture medium after pantoea agglomerans access sterilizing, under conditions of temperature is 32-36 DEG C, keynote will be cultivated
To pH value be 7.1-7.3, vacuum culture 48-56 hours；By the pantoea agglomerans and trichoderma chlamydospore bacterium, cellulose point after culture
Solution property Mixed Microbes bacterium solution, the mixing of fixing nitrogen, dissolving phosphor and dissolving potassium compound bacteria liquid, stir 34-38 minutes under conditions of 31-33 DEG C, obtain
Mixed bacteria liquid；
(3), it is put into after mixing seedling vine, wheat bran, rice straw and carries out being crushed to 43-49 mesh in pulverizer, then
The mixture obtained with N P and K base fertilizer, trace-element fertilizer, Folithion missible oil and step (1) be mixed
It is even, obtain mixture A；
(4), mixture A is spread in fermentor, the thickness of every 11-13 centimeters of paving, uniformly sprinkling step (2) obtains mixed
Combined bacteria liquid is simultaneously compacted, and repaves the second layer, and so on, until being paved with entire fermentor, fermentor is sealed anaerobic fermentation,
Obtain the compound microorganism ferments fertilizer for planting fruit trees.
Preferably, comprising the following steps:
(1), the raw material of above-mentioned parts by weight is weighed；Black pig Metabolism Excretion object is separated by solid-liquid separation, solid carries out after collecting
It dries, temperature is controlled at 39 DEG C, until water content is 26%, is then carried out UV treatment 70 minutes, is used after UV treatment
Ozone carries out sterilization processing, in sterilization process, every 14 minutes progress turning and throwing onces, sterilizes 60min altogether；By the black pig after sterilization
Metabolism Excretion object is mixed with sheep barnyard manure, mixing speed 350r/min, mixing time 110min；
(2), by the culture medium after pantoea agglomerans access sterilizing, under conditions of temperature is 34 DEG C, culture medium is adjusted to
PH value is 7.2, vacuum culture 52 hours；Pantoea agglomerans after culture is mixed with trichoderma chlamydospore bacterium, cellulolytic
Bacterium bacterium solution, the mixing of fixing nitrogen, dissolving phosphor and dissolving potassium compound bacteria liquid, stir 36 minutes under conditions of 32 DEG C, obtain mixed bacteria liquid；
(3), seedling vine, wheat bran, rice straw are put into after mixing and carry out being crushed to 46 meshes in pulverizer, then with nitrogen
The mixture that phosphorus potassium base fertilizer, trace-element fertilizer, Folithion missible oil and step (1) obtain is mixed evenly,
Obtain mixture A；
(4), mixture A is spread in fermentor, the thickness of every 12 centimeters of paving, uniformly sprays the mixing that step (2) obtain
Bacterium solution is simultaneously compacted, and repaves the second layer, and so on, until being paved with entire fermentor, fermentor is sealed anaerobic fermentation, i.e.,
Obtain the compound microorganism ferments fertilizer for planting fruit trees.
Preferably, culture medium contains soy meal 2-6%, corn flour 2-4%, glucose 0.5- in the step (2)
0.9%, yeast extract 0.2-0.6%, protein 0.3-0.7%, calcium carbonate 2-6%, surplus are water.
Preferably, the condition being mixed evenly in the step (3) are as follows: under conditions of temperature is 29-41 DEG C, stirring
Speed is 400-500r/min, and mixing time is 15-23 minutes.
Beneficial effects of the present invention:
(1), compound microorganism ferments fertilizer prepared by the present invention, be suitable for a variety of fruit trees, the present invention using pantoea agglomerans,
The addition of cellulolytic Mixed Microbes bacterium solution, fixing nitrogen, dissolving phosphor and dissolving potassium compound bacteria liquid, it is effective to decompose N P and K base fertilizer
And organic matter and degraded cellulose in trace-element fertilizer, it improves the conversion ratio of fertilizer, reduce the loss of chemical fertilizer, improve
The utilization rate of fertilizer.
(2), compound microorganism ferments fertilizer prepared by the present invention, black pig Metabolism Excretion object and sheep barnyard manure be added to for
The growth of fruit tree provides protein and nutriment, and the addition of black pig Metabolism Excretion object and sheep barnyard manure realizes waste benefit
With the addition of organic fertilizer and inorganic fertilizer in reduction fruit tree fertilizer reduces environmental pollution.
(3), in compound microorganism ferments fertilizer prepared by the present invention, Folithion missible oil is to have phosphorus pesticide, and agglomerating general
The pest and disease damage that bacterium collective effect, killing soil and fruit tree contain, the effect for being provided simultaneously with resistance, inhibiting pathogenic bacteria.
Specific embodiment
Below with reference to embodiment, further description of the specific embodiments of the present invention, and following embodiment is only used for more
Technical solution of the present invention is clearly demonstrated, and not intended to limit the protection scope of the present invention.
Embodiment 1
A kind of compound microorganism ferments fertilizer for planting fruit trees, the raw material including following parts by weight: black pig metabolism row
Let out 40 parts of object, 50 parts of seedling vine, 35 parts of sheep barnyard manure, 15 parts of wheat bran, 20 parts of rice straw, 38 parts of N P and K base fertilizer, micro member
3 parts of fertilizer, 20 parts of trichoderma chlamydospore bacterium, 10 parts of Folithion missible oil, 13 parts of pantoea agglomerans, cellulolytic Mixed Microbes bacterium
15 parts of liquid, 12 parts of fixing nitrogen, dissolving phosphor and dissolving potassium compound bacteria liquid.
N P and K base fertilizer includes nitrogenous fertilizer, phosphate fertilizer, potash fertilizer；Trace-element fertilizer includes copper, iron, manganese, zinc, boron, molybdenum.
Fixing nitrogen, dissolving phosphor and dissolving potassium compound bacteria liquid is the preparation method is as follows: potassium solubilizing bacteria, phosphate solubilizing bacteria and bacillus subtilis are distinguished
Be placed in respective culture medium and carry out inclined-plane culture 3 days, cultivation temperature be 30 DEG C, after culture by potassium solubilizing bacteria, phosphate solubilizing bacteria with
And bacillus subtilis carries out mixed bacteria liquid according to 3:2:5.
Cellulolytic Mixed Microbes bacterium solution the preparation method is as follows: cellulolytic bacterium is placed in beef extract agar
Vibration culture 2 days, obtain cellulolytic bacterial solution in culture medium；Cellulolytic fungi is placed in potato sugar fine jade
Vibration culture 4 days is carried out in rouge culture medium, obtains cellulolytic fungi bacterium solution；By cellulolytic bacterial solution and fibre
Tieing up plain decomposability fungi bacterium solution, proportionally 2:5 is mixed, and obtains cellulolytic Mixed Microbes bacterium solution.
Trichoderma chlamydospore bacterium the preparation method is as follows: trichoderma is seeded in PDA culture medium, the item for being 30 DEG C in temperature
It is cultivated 4 days under part, washes lower spore, be formulated as Trichoderma spore suspension, it is 2 that Trichoderma spore suspension, which is diluted to clump count,
It after × 10^6cfu/ml, is inoculated in maize powder medium, cultivation temperature is 25 DEG C, mixing speed 200r/min, when culture
Between be 6 days, obtain trichoderma chlamydospore bacterium.
A kind of method for preparing compound microorganism ferments fertilizer for planting fruit trees, comprising the following steps:
(1), the raw material of above-mentioned parts by weight is weighed；Black pig Metabolism Excretion object is separated by solid-liquid separation, solid carries out after collecting
It dries, temperature is controlled at 35 DEG C, until water content is 20%, is then carried out UV treatment 60 minutes, is used after UV treatment
Ozone carries out sterilization processing, in sterilization process, every 10 minutes progress turning and throwing onces, sterilizes 50min altogether；By the black pig after sterilization
Metabolism Excretion object is mixed with sheep barnyard manure, mixing speed 300r/min, mixing time 100min；
(2), by the culture medium after pantoea agglomerans access sterilizing, under conditions of temperature is 30 DEG C, culture medium is adjusted to
PH value is 7.0, vacuum culture 44 hours；Pantoea agglomerans after culture is mixed with trichoderma chlamydospore bacterium, cellulolytic
Bacterium bacterium solution, the mixing of fixing nitrogen, dissolving phosphor and dissolving potassium compound bacteria liquid, stir 32 minutes under conditions of 30 DEG C, obtain mixed bacteria liquid；
(3), seedling vine, wheat bran, rice straw are put into after mixing and carry out being crushed to 40 meshes in pulverizer, then with nitrogen
The mixture that phosphorus potassium base fertilizer, trace-element fertilizer, Folithion missible oil and step (1) obtain is mixed evenly,
Obtain mixture A；
(4), mixture A is spread in fermentor, the thickness of every 10 centimeters of paving, uniformly sprays the mixing that step (2) obtain
Bacterium solution is simultaneously compacted, and repaves the second layer, and so on, until being paved with entire fermentor, fermentor is sealed anaerobic fermentation, i.e.,
Obtain the compound microorganism ferments fertilizer for planting fruit trees.
Culture medium contains soy meal 2%, corn flour 2%, glucose 0.5%, yeast extract 0.2%, protein in step (2)
0.3%, calcium carbonate 2%, surplus is water.
The condition being mixed evenly in step (3) are as follows: under conditions of temperature is 29 DEG C, mixing speed 400r/
Min, mixing time are 15 minutes.
Embodiment 2
A kind of compound microorganism ferments fertilizer for planting fruit trees, the raw material including following parts by weight: black pig metabolism row
Let out 45 parts of object, 55 parts of seedling vine, 39 parts of sheep barnyard manure, 20 parts of wheat bran, 22 parts of rice straw, 40 parts of N P and K base fertilizer, micro member
5 parts of fertilizer, 22 parts of trichoderma chlamydospore bacterium, 12 parts of Folithion missible oil, 15 parts of pantoea agglomerans, cellulolytic Mixed Microbes bacterium
18 parts of liquid, 14 parts of fixing nitrogen, dissolving phosphor and dissolving potassium compound bacteria liquid.
N P and K base fertilizer includes nitrogenous fertilizer, phosphate fertilizer, potash fertilizer；Trace-element fertilizer includes copper, iron, manganese, zinc, boron, molybdenum.
Fixing nitrogen, dissolving phosphor and dissolving potassium compound bacteria liquid is the preparation method is as follows: potassium solubilizing bacteria, phosphate solubilizing bacteria and bacillus subtilis are distinguished
It is placed in respective culture medium and carries out inclined-plane culture 3.5 days, cultivation temperature is 32 DEG C, by potassium solubilizing bacteria, phosphate solubilizing bacteria after culture
And bacillus subtilis carries out mixed bacteria liquid according to 3:2:5.
Cellulolytic Mixed Microbes bacterium solution the preparation method is as follows: cellulolytic bacterium is placed in beef extract agar
Vibration culture 2.5 days, obtain cellulolytic bacterial solution in culture medium；Cellulolytic fungi is placed in potato sugar
Vibration culture 4.5 days is carried out in agar medium, obtains cellulolytic fungi bacterium solution；By cellulolytic bacterial solution
Proportionally 2:5 is mixed with cellulolytic fungi bacterium solution, obtains cellulolytic Mixed Microbes bacterium solution.
Trichoderma chlamydospore bacterium the preparation method is as follows: trichoderma is seeded in PDA culture medium, the item for being 32 DEG C in temperature
It is cultivated 4.5 days under part, washes lower spore, be formulated as Trichoderma spore suspension, Trichoderma spore suspension is diluted to clump count
It after 2.5 × 10^6cfu/ml, is inoculated in maize powder medium, cultivation temperature is 27 DEG C, mixing speed 215r/min, training
Supporting the time is 6.5 days, obtains trichoderma chlamydospore bacterium.
A kind of method for preparing compound microorganism ferments fertilizer for planting fruit trees, comprising the following steps:
(1), the raw material of above-mentioned parts by weight is weighed；Black pig Metabolism Excretion object is separated by solid-liquid separation, solid carries out after collecting
It dries, temperature is controlled at 37 DEG C, until water content is 23%, is then carried out UV treatment 65 minutes, is used after UV treatment
Ozone carries out sterilization processing, in sterilization process, every 12 minutes progress turning and throwing onces, sterilizes 55min altogether；By the black pig after sterilization
Metabolism Excretion object is mixed with sheep barnyard manure, mixing speed 325r/min, mixing time 105min；
(2), by the culture medium after pantoea agglomerans access sterilizing, under conditions of temperature is 32 DEG C, culture medium is adjusted to
PH value is 7.1, vacuum culture 48 hours；Pantoea agglomerans after culture is mixed with trichoderma chlamydospore bacterium, cellulolytic
Bacterium bacterium solution, the mixing of fixing nitrogen, dissolving phosphor and dissolving potassium compound bacteria liquid, stir 34 minutes under conditions of 31 DEG C, obtain mixed bacteria liquid；
(3), seedling vine, wheat bran, rice straw are put into after mixing and carry out being crushed to 43 meshes in pulverizer, then with nitrogen
The mixture that phosphorus potassium base fertilizer, trace-element fertilizer, Folithion missible oil and step (1) obtain is mixed evenly,
Obtain mixture A；
(4), mixture A is spread in fermentor, the thickness of every 11 centimeters of paving, uniformly sprays the mixing that step (2) obtain
Bacterium solution is simultaneously compacted, and repaves the second layer, and so on, until being paved with entire fermentor, fermentor is sealed anaerobic fermentation, i.e.,
Obtain the compound microorganism ferments fertilizer for planting fruit trees.
Culture medium contains soy meal 3%, corn flour 2.5%, glucose 0.6%, yeast extract 0.3%, albumen in step (2)
Matter 0.4%, calcium carbonate 3%, surplus are water.
The condition being mixed evenly in step (3) are as follows: under conditions of temperature is 32 DEG C, mixing speed 425r/
Min, mixing time are 17 minutes.
Embodiment 3
A kind of compound microorganism ferments fertilizer for planting fruit trees, the raw material including following parts by weight: black pig metabolism row
Let out 50 parts of object, 60 parts of seedling vine, 43 parts of sheep barnyard manure, 25 parts of wheat bran, 24 parts of rice straw, 42 parts of N P and K base fertilizer, micro member
7 parts of fertilizer, 24 parts of trichoderma chlamydospore bacterium, 14 parts of Folithion missible oil, 17 parts of pantoea agglomerans, cellulolytic Mixed Microbes bacterium
21 parts of liquid, 16 parts of fixing nitrogen, dissolving phosphor and dissolving potassium compound bacteria liquid.
N P and K base fertilizer includes nitrogenous fertilizer, phosphate fertilizer, potash fertilizer；Trace-element fertilizer includes copper, iron, manganese, zinc, boron, molybdenum.
Fixing nitrogen, dissolving phosphor and dissolving potassium compound bacteria liquid is the preparation method is as follows: potassium solubilizing bacteria, phosphate solubilizing bacteria and bacillus subtilis are distinguished
Be placed in respective culture medium and carry out inclined-plane culture 4 days, cultivation temperature be 34 DEG C, after culture by potassium solubilizing bacteria, phosphate solubilizing bacteria with
And bacillus subtilis carries out mixed bacteria liquid according to 3:2:5.
Cellulolytic Mixed Microbes bacterium solution the preparation method is as follows: cellulolytic bacterium is placed in beef extract agar
Vibration culture 3 days, obtain cellulolytic bacterial solution in culture medium；Cellulolytic fungi is placed in potato sugar fine jade
Vibration culture 5 days is carried out in rouge culture medium, obtains cellulolytic fungi bacterium solution；By cellulolytic bacterial solution and fibre
Tieing up plain decomposability fungi bacterium solution, proportionally 2:5 is mixed, and obtains cellulolytic Mixed Microbes bacterium solution.
Trichoderma chlamydospore bacterium the preparation method is as follows: trichoderma is seeded in PDA culture medium, the item for being 34 DEG C in temperature
It is cultivated 5 days under part, washes lower spore, be formulated as Trichoderma spore suspension, it is 3 that Trichoderma spore suspension, which is diluted to clump count,
It after × 10^6cfu/ml, is inoculated in maize powder medium, cultivation temperature is 29 DEG C, mixing speed 230r/min, when culture
Between be 7 days, obtain trichoderma chlamydospore bacterium.
A kind of method for preparing compound microorganism ferments fertilizer for planting fruit trees, comprising the following steps:
(1), the raw material of above-mentioned parts by weight is weighed；Black pig Metabolism Excretion object is separated by solid-liquid separation, solid carries out after collecting
It dries, temperature is controlled at 39 DEG C, until water content is 26%, is then carried out UV treatment 70 minutes, is used after UV treatment
Ozone carries out sterilization processing, in sterilization process, every 14 minutes progress turning and throwing onces, sterilizes 60min altogether；By the black pig after sterilization
Metabolism Excretion object is mixed with sheep barnyard manure, mixing speed 350r/min, mixing time 110min；
(2), by the culture medium after pantoea agglomerans access sterilizing, under conditions of temperature is 34 DEG C, culture medium is adjusted to
PH value is 7.2, vacuum culture 52 hours；Pantoea agglomerans after culture is mixed with trichoderma chlamydospore bacterium, cellulolytic
Bacterium bacterium solution, the mixing of fixing nitrogen, dissolving phosphor and dissolving potassium compound bacteria liquid, stir 36 minutes under conditions of 32 DEG C, obtain mixed bacteria liquid；
(3), seedling vine, wheat bran, rice straw are put into after mixing and carry out being crushed to 46 meshes in pulverizer, then with nitrogen
The mixture that phosphorus potassium base fertilizer, trace-element fertilizer, Folithion missible oil and step (1) obtain is mixed evenly,
Obtain mixture A；
(4), mixture A is spread in fermentor, the thickness of every 12 centimeters of paving, uniformly sprays the mixing that step (2) obtain
Bacterium solution is simultaneously compacted, and repaves the second layer, and so on, until being paved with entire fermentor, fermentor is sealed anaerobic fermentation, i.e.,
Obtain the compound microorganism ferments fertilizer for planting fruit trees.
Culture medium contains soy meal 4%, corn flour 3%, glucose 0.7%, yeast extract 0.4%, protein in step (2)
0.5%, calcium carbonate 4%, surplus is water.
The condition being mixed evenly in step (3) are as follows: under conditions of temperature is 35 DEG C, mixing speed 450r/
Min, mixing time are 19 minutes.
Embodiment 4
A kind of compound microorganism ferments fertilizer for planting fruit trees, the raw material including following parts by weight: black pig metabolism row
Let out 55 parts of object, 65 parts of seedling vine, 47 parts of sheep barnyard manure, 30 parts of wheat bran, 26 parts of rice straw, 44 parts of N P and K base fertilizer, micro member
9 parts of fertilizer, 26 parts of trichoderma chlamydospore bacterium, 16 parts of Folithion missible oil, 19 parts of pantoea agglomerans, cellulolytic Mixed Microbes bacterium
24 parts of liquid, 18 parts of fixing nitrogen, dissolving phosphor and dissolving potassium compound bacteria liquid.
N P and K base fertilizer includes nitrogenous fertilizer, phosphate fertilizer, potash fertilizer；Trace-element fertilizer includes copper, iron, manganese, zinc, boron, molybdenum.
Fixing nitrogen, dissolving phosphor and dissolving potassium compound bacteria liquid is the preparation method is as follows: potassium solubilizing bacteria, phosphate solubilizing bacteria and bacillus subtilis are distinguished
It is placed in respective culture medium and carries out inclined-plane culture 4.5 days, cultivation temperature is 36 DEG C, by potassium solubilizing bacteria, phosphate solubilizing bacteria after culture
And bacillus subtilis carries out mixed bacteria liquid according to 3:2:5.
Cellulolytic Mixed Microbes bacterium solution the preparation method is as follows: cellulolytic bacterium is placed in beef extract agar
Vibration culture 3.5 days, obtain cellulolytic bacterial solution in culture medium；Cellulolytic fungi is placed in potato sugar
Vibration culture 5.5 days is carried out in agar medium, obtains cellulolytic fungi bacterium solution；By cellulolytic bacterial solution
Proportionally 2:5 is mixed with cellulolytic fungi bacterium solution, obtains cellulolytic Mixed Microbes bacterium solution.
Trichoderma chlamydospore bacterium the preparation method is as follows: trichoderma is seeded in PDA culture medium, the item for being 36 DEG C in temperature
It is cultivated 5.5 days under part, washes lower spore, be formulated as Trichoderma spore suspension, Trichoderma spore suspension is diluted to clump count
It after 3.5 × 10^6cfu/ml, is inoculated in maize powder medium, cultivation temperature is 31 DEG C, mixing speed 245r/min, training
Supporting the time is 7.5 days, obtains trichoderma chlamydospore bacterium.
A kind of method for preparing compound microorganism ferments fertilizer for planting fruit trees, comprising the following steps:
(1), the raw material of above-mentioned parts by weight is weighed；Black pig Metabolism Excretion object is separated by solid-liquid separation, solid carries out after collecting
It dries, temperature is controlled at 41 DEG C, until water content is 29%, is then carried out UV treatment 75 minutes, is used after UV treatment
Ozone carries out sterilization processing, in sterilization process, every 16 minutes progress turning and throwing onces, sterilizes 65min altogether；By the black pig after sterilization
Metabolism Excretion object is mixed with sheep barnyard manure, mixing speed 375r/min, mixing time 115min；
(2), by the culture medium after pantoea agglomerans access sterilizing, under conditions of temperature is 36 DEG C, culture medium is adjusted to
PH value is 7.3, vacuum culture 56 hours；Pantoea agglomerans after culture is mixed with trichoderma chlamydospore bacterium, cellulolytic
Bacterium bacterium solution, the mixing of fixing nitrogen, dissolving phosphor and dissolving potassium compound bacteria liquid, stir 38 minutes under conditions of 33 DEG C, obtain mixed bacteria liquid；
(3), seedling vine, wheat bran, rice straw are put into after mixing and carry out being crushed to 49 meshes in pulverizer, then with nitrogen
The mixture that phosphorus potassium base fertilizer, trace-element fertilizer, Folithion missible oil and step (1) obtain is mixed evenly,
Obtain mixture A；
(4), mixture A is spread in fermentor, the thickness of every 13 centimeters of paving, uniformly sprays the mixing that step (2) obtain
Bacterium solution is simultaneously compacted, and repaves the second layer, and so on, until being paved with entire fermentor, fermentor is sealed anaerobic fermentation, i.e.,
Obtain the compound microorganism ferments fertilizer for planting fruit trees.
Culture medium contains soy meal 5%, corn flour 3.5%, glucose 0.8%, yeast extract 0.5%, albumen in step (2)
Matter 0.6%, calcium carbonate 5%, surplus are water.
The condition being mixed evenly in step (3) are as follows: under conditions of temperature is 38 DEG C, mixing speed 475r/
Min, mixing time are 21 minutes.
Embodiment 5
A kind of compound microorganism ferments fertilizer for planting fruit trees, the raw material including following parts by weight: black pig metabolism row
Let out 60 parts of object, 70 parts of seedling vine, 51 parts of sheep barnyard manure, 35 parts of wheat bran, 28 parts of rice straw, 46 parts of N P and K base fertilizer, micro member
11 parts of fertilizer, 28 parts of trichoderma chlamydospore bacterium, 18 parts of Folithion missible oil, 21 parts of pantoea agglomerans, cellulolytic Mixed Microbes
27 parts of bacterium solution, 20 parts of fixing nitrogen, dissolving phosphor and dissolving potassium compound bacteria liquid.
N P and K base fertilizer includes nitrogenous fertilizer, phosphate fertilizer, potash fertilizer；Trace-element fertilizer includes copper, iron, manganese, zinc, boron, molybdenum.
Fixing nitrogen, dissolving phosphor and dissolving potassium compound bacteria liquid is the preparation method is as follows: potassium solubilizing bacteria, phosphate solubilizing bacteria and bacillus subtilis are distinguished
Be placed in respective culture medium and carry out inclined-plane culture 5 days, cultivation temperature be 38 DEG C, after culture by potassium solubilizing bacteria, phosphate solubilizing bacteria with
And bacillus subtilis carries out mixed bacteria liquid according to 3:2:5.
Cellulolytic Mixed Microbes bacterium solution the preparation method is as follows: cellulolytic bacterium is placed in beef extract agar
Vibration culture 4 days, obtain cellulolytic bacterial solution in culture medium；Cellulolytic fungi is placed in potato sugar fine jade
Vibration culture 6 days is carried out in rouge culture medium, obtains cellulolytic fungi bacterium solution；By cellulolytic bacterial solution and fibre
Tieing up plain decomposability fungi bacterium solution, proportionally 2:5 is mixed, and obtains cellulolytic Mixed Microbes bacterium solution.
Trichoderma chlamydospore bacterium the preparation method is as follows: trichoderma is seeded in PDA culture medium, the item for being 38 DEG C in temperature
It is cultivated 6 days under part, washes lower spore, be formulated as Trichoderma spore suspension, it is 4 that Trichoderma spore suspension, which is diluted to clump count,
It after × 10^6cfu/ml, is inoculated in maize powder medium, cultivation temperature is 33 DEG C, mixing speed 260r/min, when culture
Between be 8 days, obtain trichoderma chlamydospore bacterium.
A kind of method for preparing compound microorganism ferments fertilizer for planting fruit trees, comprising the following steps:
(1), the raw material of above-mentioned parts by weight is weighed；Black pig Metabolism Excretion object is separated by solid-liquid separation, solid carries out after collecting
It dries, temperature is controlled at 43 DEG C, until water content is 32%, is then carried out UV treatment 80 minutes, is used after UV treatment
Ozone carries out sterilization processing, in sterilization process, every 18 minutes progress turning and throwing onces, sterilizes 70min altogether；By the black pig after sterilization
Metabolism Excretion object is mixed with sheep barnyard manure, mixing speed 400r/min, mixing time 120min；
(2), by the culture medium after pantoea agglomerans access sterilizing, under conditions of temperature is 38 DEG C, culture medium is adjusted to
PH value is 7.4, vacuum culture 60 hours；Pantoea agglomerans after culture is mixed with trichoderma chlamydospore bacterium, cellulolytic
Bacterium bacterium solution, the mixing of fixing nitrogen, dissolving phosphor and dissolving potassium compound bacteria liquid, stir 40 minutes under conditions of 34 DEG C, obtain mixed bacteria liquid；
(3), seedling vine, wheat bran, rice straw are put into after mixing and carry out being crushed to 52 meshes in pulverizer, then with nitrogen
The mixture that phosphorus potassium base fertilizer, trace-element fertilizer, Folithion missible oil and step (1) obtain is mixed evenly,
Obtain mixture A；
(4), mixture A is spread in fermentor, the thickness of every 14 centimeters of paving, uniformly sprays the mixing that step (2) obtain
Bacterium solution is simultaneously compacted, and repaves the second layer, and so on, until being paved with entire fermentor, fermentor is sealed anaerobic fermentation, i.e.,
Obtain the compound microorganism ferments fertilizer for planting fruit trees.
Culture medium contains soy meal 6%, corn flour 4%, glucose 0.9%, yeast extract 0.6%, protein in step (2)
0.7%, calcium carbonate 6%, surplus is water.
The condition being mixed evenly in step (3) are as follows: under conditions of temperature is 41 DEG C, mixing speed 500r/
Min, mixing time are 23 minutes.
Comparative example group
Comparative example 1
The compound microorganism ferments fertilizer and preparation method thereof that the present invention relates to a kind of for planting fruit trees, with embodiment 1
It compares, removes the step (1) in black pig Metabolism Excretion object, sheep barnyard manure and the preparation method in ingredient, remaining component and step
It is same as Example 1.
Comparative example 2
The compound microorganism ferments fertilizer and preparation method thereof that the present invention relates to a kind of for planting fruit trees, with embodiment 1
It compares, removes pantoea agglomerans in component, cellulolytic Mixed Microbes bacterium solution, fixing nitrogen, dissolving phosphor and dissolving potassium compound bacteria liquid, remaining
Component is same as Example 1 with step.
Comparative example 3
The present invention is the fertilizer of Chinese patent (patent application of Publication No. CN104609930A) preparation.
Product is carried out according to the requirement of " composite microbiological fertilizer " professional standard (NY/T798-2004) and detection method
It examines；Selection is by pesticide and the more serious soil of heavy metal pollution before plantation, and remaining pesticide averagely exceeds standard after measured
60 times, heavy metal pollution exceeds 25 times of standard, plants apple tree, on soil with the fertilizer of embodiment 1-5 and comparative example 1-3
It applies fertilizer, conventional method field management includes that the processes such as top dressing, weeding, laxative are all identical.
Below table is experimental result:
It is greater than having in comparative example 1-3 from can be seen that the living bacteria count detected in embodiment 1-5 in above table
Viable count is imitated, the total nutrient N+P205+K20 in embodiment 1-5 reaches 22.1, and miscellaneous bacteria rate is lower than 5%, and moisture is lower than 9%, compares
Parameter in comparative example 1-3 is high.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, although referring to aforementioned reality
Applying example, invention is explained in detail, for those skilled in the art, still can be to aforementioned each implementation
Technical solution documented by example is modified or equivalent replacement of some of the technical features.It is all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of compound microorganism ferments fertilizer for planting fruit trees, it is characterised in that: the raw material including following parts by weight: black
40-60 parts of pig Metabolism Excretion object, 50-70 parts of seedling vine, 35-51 parts of sheep barnyard manure, 15-35 parts of wheat bran, 20-28 parts of rice straw, nitrogen
38-46 parts of phosphorus potassium base fertilizer, 3-11 parts of trace-element fertilizer, 20-28 parts of trichoderma chlamydospore bacterium, Folithion missible oil 10-18
Part, 13-21 parts of pantoea agglomerans, 15-27 parts of cellulolytic Mixed Microbes bacterium solution, fixing nitrogen, dissolving phosphor and dissolving potassium compound bacteria liquid 12-20
Part.
2. a kind of compound microorganism ferments fertilizer for planting fruit trees according to claim 1, it is characterised in that: described
N P and K base fertilizer includes nitrogenous fertilizer, phosphate fertilizer, potash fertilizer；Trace-element fertilizer includes copper, iron, manganese, zinc, boron, molybdenum.
3. a kind of compound microorganism ferments fertilizer for planting fruit trees according to claim 1, it is characterised in that: described
Fixing nitrogen, dissolving phosphor and dissolving potassium compound bacteria liquid is the preparation method is as follows: potassium solubilizing bacteria, phosphate solubilizing bacteria and bacillus subtilis are respectively placed in respectively
Culture medium in carry out inclined-plane culture 3-5 days, cultivation temperature is 30-38 DEG C, by potassium solubilizing bacteria, phosphate solubilizing bacteria and withered after culture
Careless bacillus carries out mixed bacteria liquid according to 3:2:5.
4. a kind of compound microorganism ferments fertilizer for planting fruit trees according to claim 1, it is characterised in that: described
Cellulolytic Mixed Microbes bacterium solution the preparation method is as follows: cellulolytic bacterium is placed in beef extract agar medium
Vibration culture 2-4 days, obtains cellulolytic bacterial solution；Cellulolytic fungi is placed in potato sugar agar culture
Vibration culture 4-6 days is carried out in base, obtains cellulolytic fungi bacterium solution；By cellulolytic bacterial solution and cellulose
Proportionally 2:5 is mixed decomposability fungi bacterium solution, obtains cellulolytic Mixed Microbes bacterium solution.
5. a kind of compound microorganism ferments fertilizer for planting fruit trees according to claim 1, it is characterised in that: described
Trichoderma chlamydospore bacterium the preparation method is as follows: trichoderma is seeded in PDA culture medium, under conditions of temperature is 30-38 DEG C
Culture 4-6 days, washes lower spore, is formulated as Trichoderma spore suspension, Trichoderma spore suspension is diluted to clump count as 2 ×
It after 10^6-4 × 10^6cfu/ml, is inoculated in maize powder medium, cultivation temperature is 25-33 DEG C, mixing speed 200-
260r/min, incubation time are 6-8 days, obtain trichoderma chlamydospore bacterium.
6. preparing the side of any compound microorganism ferments fertilizer for planting fruit trees described in -5 according to claim 1
Method, it is characterised in that: the following steps are included:
(1), the raw material of above-mentioned parts by weight is weighed；Black pig Metabolism Excretion object is separated by solid-liquid separation, solid is dried after collecting,
Temperature is controlled at 35-43 DEG C, until water content is 20-32%, is then carried out UV treatment 60-80 minutes, after UV treatment
Sterilization processing is carried out using ozone, in sterilization process, every 10-18 minutes progress turning and throwing onces, sterilizes 50-70min altogether；It will kill
Black pig Metabolism Excretion object after bacterium is mixed with sheep barnyard manure, mixing speed 300-400r/min, and mixing time is
100-120min；
(2), by the culture medium after pantoea agglomerans access sterilizing, under conditions of temperature is 30-38 DEG C, culture medium is adjusted to pH
Being worth is 7.0-7.4, vacuum culture 44-60 hours；By after culture pantoea agglomerans and trichoderma chlamydospore bacterium, cellulolytic
Mixed Microbes bacterium solution, the mixing of fixing nitrogen, dissolving phosphor and dissolving potassium compound bacteria liquid, stir 32-40 minutes under conditions of 30-34 DEG C, are mixed
Bacterium solution；
(3), seedling vine, wheat bran, rice straw are put into after mixing and carry out being crushed to 40-52 mesh in pulverizer, then with nitrogen
The mixture that phosphorus potassium base fertilizer, trace-element fertilizer, Folithion missible oil and step (1) obtain is mixed evenly,
Obtain mixture A；
(4), mixture A is spread in fermentor, the thickness of every 10-14 centimeters of paving, uniformly sprays the Mixed Microbes that step (2) obtain
Liquid is simultaneously compacted, and repaves the second layer, and so on, until be paved with entire fermentor, by fermentor be sealed anaerobic fermentation to get
To the compound microorganism ferments fertilizer for planting fruit trees.
7. a kind of method according to claim 6 for preparing compound microorganism ferments fertilizer for planting fruit trees, special
Sign is: the following steps are included:
(1), the raw material of above-mentioned parts by weight is weighed；Black pig Metabolism Excretion object is separated by solid-liquid separation, solid is dried after collecting,
Temperature is controlled at 37-41 DEG C, until water content is 23-29%, is then carried out UV treatment 65-75 minutes, after UV treatment
Sterilization processing is carried out using ozone, in sterilization process, every 12-16 minutes progress turning and throwing onces, sterilizes 55-65min altogether；It will kill
Black pig Metabolism Excretion object after bacterium is mixed with sheep barnyard manure, mixing speed 325-375r/min, and mixing time is
105-115min；
(2), by the culture medium after pantoea agglomerans access sterilizing, under conditions of temperature is 32-36 DEG C, culture medium is adjusted to pH
Being worth is 7.1-7.3, vacuum culture 48-56 hours；By after culture pantoea agglomerans and trichoderma chlamydospore bacterium, cellulolytic
Mixed Microbes bacterium solution, the mixing of fixing nitrogen, dissolving phosphor and dissolving potassium compound bacteria liquid, stir 34-38 minutes under conditions of 31-33 DEG C, are mixed
Bacterium solution；
(3), seedling vine, wheat bran, rice straw are put into after mixing and carry out being crushed to 43-49 mesh in pulverizer, then with nitrogen
The mixture that phosphorus potassium base fertilizer, trace-element fertilizer, Folithion missible oil and step (1) obtain is mixed evenly,
Obtain mixture A；
(4), mixture A is spread in fermentor, the thickness of every 11-13 centimeters of paving, uniformly sprays the Mixed Microbes that step (2) obtain
Liquid is simultaneously compacted, and repaves the second layer, and so on, until be paved with entire fermentor, by fermentor be sealed anaerobic fermentation to get
To the compound microorganism ferments fertilizer for planting fruit trees.
8. a kind of method according to claim 6 for preparing compound microorganism ferments fertilizer for planting fruit trees, special
Sign is: the following steps are included:
(1), the raw material of above-mentioned parts by weight is weighed；Black pig Metabolism Excretion object is separated by solid-liquid separation, solid is dried after collecting,
Temperature control at 39 DEG C, until water content be 26%, then carry out UV treatment 70 minutes, after UV treatment using ozone into
Row sterilization processing in sterilization process, every 14 minutes progress turning and throwing onces, sterilizes 60min altogether；Black pig after sterilization is metabolized row
It lets out object to be mixed with sheep barnyard manure, mixing speed 350r/min, mixing time 110min；
(2), by the culture medium after pantoea agglomerans access sterilizing, under conditions of temperature is 34 DEG C, culture medium is adjusted to pH value
It is 7.2, vacuum culture 52 hours；By after culture pantoea agglomerans and trichoderma chlamydospore bacterium, cellulolytic Mixed Microbes bacterium
Liquid, the mixing of fixing nitrogen, dissolving phosphor and dissolving potassium compound bacteria liquid, stir 36 minutes under conditions of 32 DEG C, obtain mixed bacteria liquid；
(3), seedling vine, wheat bran, rice straw are put into after mixing and carry out being crushed to 46 meshes in pulverizer, then with N P and K
The mixture that base fertilizer, trace-element fertilizer, Folithion missible oil and step (1) obtain is mixed evenly, and is obtained
Mixture A；
(4), mixture A is spread in fermentor, the thickness of every 12 centimeters of paving, uniformly sprays the mixed bacteria liquid that step (2) obtain
And be compacted, the second layer is repaved, and so on, until being paved with entire fermentor, fermentor is sealed anaerobic fermentation to get arriving
Compound microorganism ferments fertilizer for planting fruit trees.
9. a kind of method according to claim 6 for preparing compound microorganism ferments fertilizer for planting fruit trees, special
Sign is: culture medium contains soy meal 2-6%, corn flour 2-4%, glucose 0.5-0.9%, yeast extract in the step (2)
0.2-0.6%, protein 0.3-0.7%, calcium carbonate 2-6%, surplus are water.
10. a kind of method according to claim 6 for preparing compound microorganism ferments fertilizer for planting fruit trees, special
Sign is: the condition being mixed evenly in the step (3) are as follows: under conditions of temperature is 29-41 DEG C, mixing speed is
400-500r/min, mixing time are 15-23 minutes.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109734531A (en) * | 2019-03-16 | 2019-05-10 | 刘超 | It is a kind of for prevent and treat ginkgo leaf yellow leaf it is withered medicine fertilizer and its fertilizing method |
| WO2022031845A1 (en) * | 2020-08-04 | 2022-02-10 | Cytozyme Laboratories, Inc. | Plant fertilizer compositions and related methods for enhancing phosphorus solubility |
-
2018
- 2018-11-22 CN CN201811396409.4A patent/CN109369266A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109734531A (en) * | 2019-03-16 | 2019-05-10 | 刘超 | It is a kind of for prevent and treat ginkgo leaf yellow leaf it is withered medicine fertilizer and its fertilizing method |
| WO2022031845A1 (en) * | 2020-08-04 | 2022-02-10 | Cytozyme Laboratories, Inc. | Plant fertilizer compositions and related methods for enhancing phosphorus solubility |
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