CN109329057A - The tissue culture mating system that ground is harvested - Google Patents
The tissue culture mating system that ground is harvested Download PDFInfo
- Publication number
- CN109329057A CN109329057A CN201811280002.5A CN201811280002A CN109329057A CN 109329057 A CN109329057 A CN 109329057A CN 201811280002 A CN201811280002 A CN 201811280002A CN 109329057 A CN109329057 A CN 109329057A
- Authority
- CN
- China
- Prior art keywords
- culture
- harvested
- tissue culture
- days
- ground
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses the tissue culture mating systems that ground is harvested, comprising the following steps: (1) explant is handled, (2) callus tissue culture, (3) bud culture is lured, (4) shoot proliferation culture, (5) culture of rootage, (6) hardening.The tissue culture mating system harvested of the present inventionly, cultivation period is short, harvest that seedling is best in quality, high survival rate in obtained ground, be transplanted to adapt to after natural environment environment ability it is strong, pest and disease damage is few, the drought-resistant and cold performance that the earth is harvested is helped to improve, the yield harvested with guaranteeing, while the tissue cultivating technology to be further improved harvested provides reference.
Description
Technical field
The present invention relates to breed of crop technical field, the tissue culture mating system harvested with being specifically related to.
Background technique
Ground harvest (Latin literary fame:Melastoma dodecandrum Lour.), also known as: shop floor, mountainous region M.-dodecandrum, lower margin M.-dodecandrum,
It is the shape undershrub that crawls of Melastomataceae, Melastoma, it is 10-30 centimetres long；It is later hairless by strigose when young；Blade heavily fortified point paper
Matter, elliptical or oval shape；Cyme, basidixed, petal lilac red to aubergine, water chestnut shape obovate, the month at florescence 5-7, fruiting period
The 7-9 month.Ground harvest be it is a kind of integrate medicinal, ornamental and health care excellent ground cover plant, it is spherical berry that fruit is harvested on ground, directly
For diameter in 0.5-1.5cm, annual 7-9 month is in batches mature, mellow fruit crust darkviolet, and pulp is sour-sweet to take on a red color and good
Good natural red colouring matter raw material.Produce Guizhou, Hunan, Guangxi, Guangdong, Jiangxi, Zhejiang, Fujian, be born in 1250 meters of height above sea level it is below
It is the common plant of acid soil in the bottom grass clump of hillside.
Ground is harvested with promoting blood circulation and hemostasis, antipyretic and antidote functions, and fruit is also edible and makes wine.Harvest adaptable, growth speed in ground
Degree is fast, due to drought-enduring resistance to lean, can low level management, leaf, flower, fruit all have preferable ornamental value, by people in afforestation
Favor, can be used as pionner plantation in slope of highway, soil erosion region and the torrid zone and subtropical area spaciouslyly and woods
Lower greening.Ground is harvested research and is utilized in early days primarily as medicinal herbs by people, sweet in flavor, puckery, cool in nature, has promoting blood circulation and hemostasis, disappears
Swollen dissolving stasis, it is clearing heat and detoxicating the effect of.It is clinically used for treatment high fever, swelling and pain, abscess of throat, toothache, red white bloody flux disease, yellow subcutaneous ulcer, water
Swollen, dysmenorrhea, leukorrhagia, postpartum abdominal pain, carbuncle swells, treats the illnesss such as sore, hemorrhoid, venomous snake bite at metrorrhagia and metrostaxis.Modern clinical research report, can
Ground is harvested, preparation is made, treat hemorrhage of digestive tract, hemostasia effect is significant.According to another report, antitumor, anti-aging, drop have been harvested in ground
The effects of blood glucose, reducing blood lipid, and there is no toxic side effect to normal cell.
People harvest the comprehensive utilization of fruit with increasingly paying close attention to and study in recent years.It is a kind of succulence berry that fruit is harvested on ground,
Subsphaeroidal, 7~11mm of diameter, reduced sugar accounts for the 96.34% of total reducing sugar in mellow fruit；Ascorbic content and tradition in fruit
Fruit pears, banana etc. are suitable, and the content of its anthocyanin is all higher than most of fruit, reaches 300mg/kg.Harvest fruit in ground
In at least contain 16 kinds of amino acid, including threonine, valine, methionine, leucine, isoleucine, phenylalanine, lysine
It is 2 times or so of rice and wheat content Deng 7 kinds of human body institute essential amino acids.Glutamic acid content with higher, and required ammonia
Base acid accounts for total amino acid content 32.80%.It harvests in fruit containing a variety of mineral members such as potassium, calcium, phosphorus, magnesium, sodium, iron, manganese, copper, zinc on ground
Element, and the ratio of each mineral element is appropriate, fruit has the characteristics that hyperkalemia hyponatremia, is conducive to the acid-base balance for maintaining body.Together
When, the content of macroelement calcium is higher, reaches 7328.4mg/kg, can be used as natural supplementary calcium food.Fruit is harvested on ground not only can be direct
It is edible, natural pigment can also be extracted for food processing, the pigment that fruit is harvested on ground has no toxic side effect, and color value is up to 190, has dense
Strongly fragrant fruit flavor, bright-colored in acid condition, property is more stable, can be used for beverage, cold drink, fruit wine, candy and dessert
Coloring.Its pigment water or ethyl alcohol extract, simple process and low cost, are a kind of excellent natural food colours.
In its natural state, ground is harvested mainly by seminal propagation, but setting percentage is not high, and seed collection is difficult, sprouts from seed
It is dealt into the time that plant blossom generally requires 2-4.There are also the artificial cultivation methods harvested about ground in recent years, at present
Harvesting main mating system has: seminal propagation, cutting propagation, piecemeal breeding and tissue culture propagation, but the tissue culture breeding harvested about ground
Side is rarely found relevant document, the main reason is that the reality that the tissue culture mating system that ground is harvested is magnified not yet is answered
With.It is original fewer with wildly harvesting, and the idea for the comprehensive utilization that people harvest ground is more and more stronger, especially harvests fruit
Development and utilization it is more and more welcomed by the people, the artificial cultivation harvested for ground is imperative, and sufficient product are provided for it
It is also extremely important that seedling is harvested on good ground of fine quality.
Summary of the invention
In view of the deficiencies of the prior art, it is an object of the present invention to provide the tissue culture mating system harvested a kind ofly, harvest
Extension artificial cultivation provides sufficient ground best in quality and harvests seedling.
The invention is realized by the following technical scheme:
The tissue culture mating system that ground is harvested, including with step:
(1) explant is handled: when in 3~April harvesting rudiment, axillary bud is harvested as explant, with nothing in the ground for acquiring no disease and pests harm
Bacterium water was rinsed well, then with alcohol disinfecting 20~30 seconds of 70~75%, was then used aseptic water washing 2~3 times, spare after dry；
(2) callus tissue culture: in an aseptic environment, axillary bud being put into callus tissue culture base and is cultivated, in temperature (25 ± 2)
DEG C, cultivate under conditions of 2600~3000lux of intensity of illumination, light application time is 8~12h/d, is cultivated 7~10 days, and callus is obtained
Tissue；
(3) it lures bud culture: in an aseptic environment, continuing culture in callus tissue culture base 10~13 days, cultivation temperature (25 ±
2) DEG C, 2800~3100lux of intensity of illumination, to the end of differentiating clump bud；
(4) shoot proliferation culture: being transferred to subculture multiplication medium and continue to cultivate, culture environment: 25~28 DEG C of temperature, illumination is strong
Spend 2800~3100lux, light application time 12~14h/ days, incubation time 3~7 days；
(5) culture of rootage: going to and carry out culture of rootage on root media, condition of culture: 25 ± 2 DEG C of temperature, intensity of illumination
3000~3200lux；Culture 8~10 days, the root long to most of seedling reaches 1~3cm, and radical 3~5 can terminate；
(6) hardening: use greenhouse seedbed hardening, medium of seedling bed peat soil in mass ratio: river sand is 1:0.3~0.5, transplanting
It is placed on shady place to seedbed, and pours appropriate root water, is transferred at sunlight after Miao Dinggen, continues culture 25 under natural conditions
~30 days, hardening was completed.
The callus tissue culture based formulas described in step (2) and (3) are as follows: MS+0.15~0.50mg/L 6-BA+
The agar of the sucrose+0.6% of 0.1~0.15mg/L NAA+2%.
Subculture multiplication medium formula described in step (4) are as follows: MS+0.15~0.50mg/L 6-BA+0.1~
The agar of the seaweeds+0.10% of the sucrose+0.12% of 0.15mg/L NAA+2.5%.
Step (5) described prescription of rooting medium are as follows: 1/2MS+0.1~+0.08~0.13mg/L of 0.15mg/L NAA
The agar of the seaweeds+1.2% of the sucrose+0.15% of IBA+3%.
Hardening process choosing bog soil and river sand of the present invention by appropriate proportion mixing as the medium of seedling bed harvested, native soil
Charcoal contains a large amount of organic matters, and loose porous, permeability is good, and retain water and nutrients power is again strong, harvests the growth of seedling with being conducive to, and can subtract
The use of few fertilizer adds a small amount of river sand mixing, is more advantageous to the hydrophobic, air-permeability in seedbed, can promote plant respiration, be conducive to
The root system development that ground is harvested, the basic no disease and pests harm of the seedling cultivated, high survival rate, the survival rate after also improving transplanting.
The seaweeds being added in culture medium contain various plants somatomedin and mineral element, chelated metal ions and
Marine biomaterial, such as cytokinin, algal polysaccharides, rich in the growth of the various plants such as calcium, magnesium, iron, zinc, copper, manganese
Necessary middle microelement integrates nutritional ingredient necessary to plant, marine bioactivity ingredient, seaweed organic matter.
Seaweeds can promote plant cell quickly to divide, fast-growth, enhancing is metabolic, raising plant biological is drought-resistant and winter resistance
Can, also have the function of inhibiting harmful organism, alleviate disease pest harm.
Beneficial effects of the present invention:
1, the tissue culture mating system harvested of the present inventionly, cultivation period is short, and obtained ground harvests that seedling is best in quality, survival rate
Height, be transplanted to adapt to after natural environment environment ability it is strong, pest and disease damage is few, the yield harvested with helping to ensure that, while for into one
It walks the tissue cultivating technology harvested with improvement and reference is provided.
2, the tissue culture mating system harvested of the present inventionly strictly optimizes by reasonable disposition culture medium and controls cultivation
Training condition, up to 82% or more, 87% or more sprout-induction rate, 92% or more root induction rate survives callus formation rate after transplanting
Rate is up to 92%.
3, the tissue culture mating system harvested to present invention research can carry out high-volume by special technical staff or mechanism
Cultivation harvests for high-volume milpa and provides enough seedling sources.
4, the seaweeds added in culture medium of the present invention can promote plant cell quickly to divide, fast-growth, enhance newly
Old metabolism, the drought-resistant and cold performance harvested with improving.
Specific embodiment
In order to keep technical solution of the present invention and advantage clearer, below with reference to the embodiment of the present invention, to the present invention
Technical solution be clearly and completely described.
Embodiment 1
The tissue culture mating system that ground is harvested, including with step:
(1) explant is handled: when in April harvesting rudiment, axillary bud is harvested as explant in the ground for acquiring no disease and pests harm, uses sterile water
It rinses well, then with alcohol disinfecting 30 seconds of 70%, then uses aseptic water washing 2 times, it is spare after dry；
(2) callus tissue culture: in an aseptic environment, axillary bud being put into callus tissue culture base and is cultivated, in temperature (25 ± 2)
DEG C, cultivate under conditions of intensity of illumination 3000lux, light application time 10h/d is cultivated 8 days, obtains callus；
(3) it lures bud culture: in an aseptic environment, continuing culture 11 days, cultivation temperature (25 ± 2) in callus tissue culture base
DEG C, intensity of illumination 2800lux, to the end of differentiating clump bud；
(4) shoot proliferation culture: being transferred to subculture multiplication medium and continue to cultivate, culture environment: 25 DEG C of temperature, intensity of illumination
2800lux, light application time 14h/ days, incubation time 3 days；
(5) culture of rootage: going to and carry out culture of rootage on root media, condition of culture: 25 ± 2 DEG C of temperature, intensity of illumination
3000lux；Culture 10 days, the root long to most of seedling reaches 1~3cm, and radical 3~5 can terminate；
(6) hardening: greenhouse seedbed hardening is used, medium of seedling bed peat soil in mass ratio: river sand 1:0.4, transplanting to seedling
Bed is placed on shady place, and pours appropriate root water, is transferred at sunlight after Miao Dinggen, continues culture 28 days under natural conditions,
Hardening is completed.
The callus tissue culture based formulas described in step (2) and (3) are as follows: MS+0.18mg/L 6-BA+0.12mg/
The agar of the sucrose+0.6% of L NAA+2%.
Subculture multiplication medium formula described in step (4) are as follows: MS+0.18mg/L 6-BA+0.1mg/L NAA+
The agar of the seaweeds+0.10% of 2.5% sucrose+0.12%.
Step (5) described prescription of rooting medium are as follows: the sucrose of 1/2MS+0.1mg/L NAA+0.1mg/L IBA+3%
The agar of+0.15% seaweeds+1.2%.
Embodiment 2
The tissue culture mating system that ground is harvested, including with step:
(1) explant is handled: when in April harvesting rudiment, axillary bud is harvested as explant in the ground for acquiring no disease and pests harm, uses sterile water
It rinses well, then with alcohol disinfecting 20 seconds of 75%, then uses aseptic water washing 3 times, it is spare after dry；
(2) callus tissue culture: in an aseptic environment, axillary bud being put into callus tissue culture base and is cultivated, in temperature (25 ± 2)
DEG C, cultivate under conditions of intensity of illumination 2600lux, light application time 12h/d is cultivated 7 days, obtains callus；
(3) it lures bud culture: in an aseptic environment, continuing culture 10 days, cultivation temperature (25 ± 2) in callus tissue culture base
DEG C, intensity of illumination 3100lux, to the end of differentiating clump bud；
(4) shoot proliferation culture: being transferred to subculture multiplication medium and continue to cultivate, culture environment: 26 DEG C of temperature, intensity of illumination
3100lux, light application time 12h/ days, incubation time 7 days；
(5) culture of rootage: going to and carry out culture of rootage on root media, condition of culture: 25 ± 2 DEG C of temperature, intensity of illumination
3200lux；Culture 8 days, the root long to most of seedling reaches 1~3cm, and radical 3~5 can terminate；
(6) hardening: greenhouse seedbed hardening is used, medium of seedling bed peat soil in mass ratio: river sand 1:0.35, transplanting to seedling
Bed is placed on shady place, and pours appropriate root water, is transferred at sunlight after Miao Dinggen, continues culture 29 days under natural conditions,
Hardening is completed.
The callus tissue culture based formulas described in step (2) and (3) are as follows: MS+0.16mg/L 6-BA+0.12mg/
The agar of the sucrose+0.6% of L NAA+2%.
Subculture multiplication medium formula described in step (4) are as follows: MS+0.16mg/L 6-BA+0.1mg/L NAA+
The agar of the seaweeds+0.10% of 2.5% sucrose+0.12%.
Step (5) described prescription of rooting medium are as follows: the sucrose of 1/2MS+0.1mg/L NAA+0.1mg/L IBA+3%
The agar of+0.15% seaweeds+1.2%.
Embodiment 3
The tissue culture mating system that ground is harvested, including with step:
(1) explant is handled: when in April harvesting rudiment, axillary bud is harvested as explant in the ground for acquiring no disease and pests harm, uses sterile water
It rinses well, then with alcohol disinfecting 30 seconds of 70%, then uses aseptic water washing 2 times, it is spare after dry；
(2) callus tissue culture: in an aseptic environment, axillary bud being put into callus tissue culture base and is cultivated, in temperature (25 ± 2)
DEG C, cultivate under conditions of intensity of illumination 2800lux, light application time 8h/d is cultivated 10 days, obtains callus；
(3) it lures bud culture: in an aseptic environment, continuing culture 13 days, cultivation temperature (25 ± 2) in callus tissue culture base
DEG C, intensity of illumination 3000lux, to the end of differentiating clump bud；
(4) shoot proliferation culture: being transferred to subculture multiplication medium and continue to cultivate, culture environment: 26 DEG C of temperature, intensity of illumination
3000lux, light application time 13h/ days, incubation time 5 days；
(5) culture of rootage: going to and carry out culture of rootage on root media, condition of culture: 25 ± 2 DEG C of temperature, intensity of illumination
3100lux；Culture 10 days, the root long to most of seedling reaches 1~3cm, and radical 3~5 can terminate；
(6) hardening: greenhouse seedbed hardening is used, medium of seedling bed peat soil in mass ratio: river sand 1:0.4, transplanting to seedling
Bed is placed on shady place, and pours appropriate root water, is transferred at sunlight after Miao Dinggen, continues culture 28 days under natural conditions,
Hardening is completed.
The callus tissue culture based formulas described in step (2) and (3) are as follows: MS+0.20mg/L 6-BA+0.12mg/
The agar of the sucrose+0.6% of L NAA+2%.
Subculture multiplication medium formula described in step (4) are as follows: MS+0.20mg/L 6-BA+0.12mg/L NAA+
The agar of the seaweeds+0.10% of 2.5% sucrose+0.12%.
Step (5) described prescription of rooting medium are as follows: the sucrose of 1/2MS+0.1mg/L NAA+0.1mg/L IBA+3%
The agar of+0.15% seaweeds+1.2%.
The following are the ground in the embodiment of the present invention to harvest seedling tissue culture test result:
As can be seen from the above table: the tissue culture mating system harvested of the inventionly has good rooting rate and transplanting survival rate,
Wherein field-transplanting survival rate illustrates that it has the preferable ability for resisting external environment variation 92% or more.Present invention training
It is shorter to educate the period, and seedling quality is high, can for the extension artificial cultivation harvested the ground best in quality of abundance be provided harvest seedling.
Claims (4)
1. the tissue culture mating system that ground is harvested, which is characterized in that including with step:
(1) explant is handled: when in 3~April harvesting rudiment, axillary bud is harvested as explant, with nothing in the ground for acquiring no disease and pests harm
Bacterium water was rinsed well, then with alcohol disinfecting 20~30 seconds of 70~75%, was then used aseptic water washing 2~3 times, spare after dry；
(2) callus tissue culture: in an aseptic environment, axillary bud is put into callus tissue culture base and is cultivated, in temperature 25 ± 2
DEG C, cultivate under conditions of 2600~3000lux of intensity of illumination, light application time is 8~12h/d, is cultivated 7~10 days, and callus is obtained
Tissue；
(3) it lures bud culture: in an aseptic environment, continuing culture in callus tissue culture base 10~13 days, cultivation temperature 25 ± 2
DEG C, 2800~3100lux of intensity of illumination, to the end of differentiating clump bud；
(4) shoot proliferation culture: being transferred to subculture multiplication medium and continue to cultivate, culture environment: 25~28 DEG C of temperature, illumination is strong
Spend 2800~3100lux, light application time 12~14h/ days, incubation time 3~7 days；
(5) culture of rootage: going to and carry out culture of rootage on root media, condition of culture: 25 ± 2 DEG C of temperature, intensity of illumination
3000~3200lux；Culture 8~10 days, the root long to most of seedling reaches 1~3cm, and radical 3~5 can terminate；
(6) hardening: use greenhouse seedbed hardening, medium of seedling bed peat soil in mass ratio: river sand is 1:0.3~0.5, transplanting
It is placed on shady place to seedbed, and pours appropriate root water, is transferred at sunlight after Miao Dinggen, continues culture 25 under natural conditions
~30 days, hardening was completed.
2. the tissue culture mating system harvested according to claim 1, which is characterized in that callus group described in step (2) and (3)
Knit culture medium prescription are as follows: the fine jade of MS+0.15~0.50mg/L+0.1~0.15mg/L of 6-BA NAA+2% sucrose+0.6%
Rouge.
3. the tissue culture mating system harvested according to claim 1, which is characterized in that the training of shoot proliferation described in step (4)
Support based formulas are as follows: the seaweed of MS+0.15~0.50mg/L+0.1~0.15mg/L of 6-BA NAA+2.5% sucrose+0.12%
The agar of element+0.10%.
4. the tissue culture mating system harvested according to claim 1, which is characterized in that root media described in step (5)
Formula are as follows: the seaweeds of 1/2MS+0.1~0.15mg/L+0.08~0.13mg/L of NAA IBA+3% sucrose+0.15%+
1.2% agar.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811280002.5A CN109329057A (en) | 2018-10-30 | 2018-10-30 | The tissue culture mating system that ground is harvested |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811280002.5A CN109329057A (en) | 2018-10-30 | 2018-10-30 | The tissue culture mating system that ground is harvested |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109329057A true CN109329057A (en) | 2019-02-15 |
Family
ID=65312667
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811280002.5A Pending CN109329057A (en) | 2018-10-30 | 2018-10-30 | The tissue culture mating system that ground is harvested |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109329057A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115968783A (en) * | 2023-01-03 | 2023-04-18 | 北京艾比蒂生物科技有限公司 | Rapid propagation method of euphorbia pekinensis |
| CN117178888A (en) * | 2023-09-03 | 2023-12-08 | 华南农业大学 | Efficient in-vitro regeneration method for pedicel and oyster leaves of Pink flower |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106518278A (en) * | 2016-09-27 | 2017-03-22 | 日照益康有机农业科技发展有限公司 | Selenium enriched algae bioorganic fertilizer and preparation method thereof |
| CN106747918A (en) * | 2016-11-22 | 2017-05-31 | 山东康德源农业科技有限公司 | Leaf vegetables nutrient solution and its application containing three kinds of form nitrogens and seaweeds |
-
2018
- 2018-10-30 CN CN201811280002.5A patent/CN109329057A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106518278A (en) * | 2016-09-27 | 2017-03-22 | 日照益康有机农业科技发展有限公司 | Selenium enriched algae bioorganic fertilizer and preparation method thereof |
| CN106747918A (en) * | 2016-11-22 | 2017-05-31 | 山东康德源农业科技有限公司 | Leaf vegetables nutrient solution and its application containing three kinds of form nitrogens and seaweeds |
Non-Patent Citations (6)
| Title |
|---|
| S. VINOTH等: "Erratum to: Effect of seaweed extracts and plant growth regulators on high-frequency in vitro mass propagation of Lycopersicon esculentum L (tomato) through double cotyledonary nodal explant", 《JOURNAL OF APPLIED PHYCOLOGY 》 * |
| 全国农技高师教育研究会《农业实用新技术大全》编委会: "《农业实用新技术大全》", 31 August 1994, 中国农业出版社 * |
| 戴小英等: "铺地锦组培快速繁殖研究", 《江西林业科技》 * |
| 杨利平等: "《园林花卉学（全彩版）》", 31 July 2017, 中国农业大学出版社 * |
| 蒋道松等: "地菍离体培养植株再生及其栽培试验", 《园艺学报》 * |
| 马国华等: "野牡丹和地稔的组织培养及植株再生", 《植物生理学通讯》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115968783A (en) * | 2023-01-03 | 2023-04-18 | 北京艾比蒂生物科技有限公司 | Rapid propagation method of euphorbia pekinensis |
| CN117178888A (en) * | 2023-09-03 | 2023-12-08 | 华南农业大学 | Efficient in-vitro regeneration method for pedicel and oyster leaves of Pink flower |
| CN117178888B (en) * | 2023-09-03 | 2024-06-04 | 华南农业大学 | Efficient in-vitro regeneration method for pedicel and oyster leaves of Pink flower |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101897309A (en) | Environmentally friendly breeding method of ricefield eel and loach | |
| CN105340510A (en) | Quinoa high-yield close-planting cultivation method and quinoa tea preparation method | |
| CN105684805A (en) | Tea tree plantation method | |
| CN105524856A (en) | Production method of bio-transformed selenium preparation and production method of selenium-enriched agricultural product | |
| CN107494345A (en) | A kind of ecological cultivation method of swamp eel | |
| KR20170079322A (en) | Cultivation method of termite mushroom of using selenium | |
| CN102498857B (en) | Okra planting method for improving active polysaccharide content though induction | |
| CN112075296A (en) | Planting method of selenium-rich proteinaria | |
| CN105532234A (en) | Method for cultivating red-pulp hylocereus polyrhizus | |
| CN109329057A (en) | The tissue culture mating system that ground is harvested | |
| CN108901858B (en) | Rapid propagation method for selenium-rich protospecies of zingiber officinale roscoe | |
| CN106258375A (en) | A kind of breeding method of potted plant Passifolra edulis | |
| CN103229659A (en) | Artificial cultivation method for oldham gypsophila and application thereof | |
| CN1176571C (en) | Cultivation of new variety of santengzhumiguo fruit and its natural colouring matter extraction method | |
| CN103636506B (en) | method for performing plant culture by utilizing shepherdia argentea caulicle regenerated plant induction culture medium and | |
| CN108293589A (en) | A kind of cultural method of collybia albuminosa | |
| CN103621405A (en) | Artificial corychophramus violaceua seed making method | |
| CN1168381C (en) | Ecological breeding and ecological culturing method for Chinese fine hair chela crab | |
| CN109258276A (en) | A kind of harvesting fruit yield artificial cultivation method with improving | |
| CN106258282A (en) | The cultural method of Radix hemerocalis plicatae is interplanted under apricot | |
| CN104488712B (en) | A kind of tissue culture and rapid propagation method of tender shoots Camellia nitidissima Chi | |
| CN1284442C (en) | Method for cultivating three-prism candied fruit novel variety by nuclear gene substitution | |
| CN104871976A (en) | Tissue culture and propagation method for spring bamboo shoots | |
| CN108651253A (en) | Sprout cultivating oxygen enrichment technology | |
| CN113079969B (en) | Method for improving survival rate of separated seedlings of water chestnut shoots planted in forests |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190215 |
|
| WD01 | Invention patent application deemed withdrawn after publication |