CN109328229A - 基因测序芯片及其安装框 - Google Patents

基因测序芯片及其安装框 Download PDF

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Publication number
CN109328229A
CN109328229A CN201680086902.9A CN201680086902A CN109328229A CN 109328229 A CN109328229 A CN 109328229A CN 201680086902 A CN201680086902 A CN 201680086902A CN 109328229 A CN109328229 A CN 109328229A
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gene sequencing
sequencing chip
chip
runner
gene
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CN109328229B (zh
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徐洪
张松振
代冲冲
郑欢
甘勇
王景
倪鸣
谭大喜
张文宇
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MGI Tech Co Ltd
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
    • B01L9/527—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips for microfluidic devices, e.g. used for lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
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  • Microbiology (AREA)
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  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

本发明属于基因测序耗材技术领域，公开了一种基因测序芯片，其包括硅片和透光盖片，硅片上设有接头区域，接头区域设有可捕获待测序基因分子的接头，透光盖片贴合于硅片上，透光盖片与硅片贴合的一面设有流道槽和密封胶槽，流道槽与硅片表面围合成流道，流道可供液体流过，接头区域位于流道内，硅片设有由外部连通至流道内的液体入口和液体出口；密封胶槽与硅片表面围合成密封胶道，密封胶道内填充有密封胶，密封胶道围绕流道。该芯片的流道四周接合面少，密封效果好，密封胶与流道分离，液体流速均匀、压力稳定，可以有效提高测序结果的准确度。本发明还提供了上述基因测序芯片的安装框，可以用于保护基因测序芯片，也可以用于辅助定位。

Description

PCT国内申请，说明书已公开。

Claims (4)

  1. PCT国内申请，权利要求书已公开。
CN201680086902.9A 2016-11-01 2016-11-01 基因测序芯片及其安装框 Active CN109328229B (zh)

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PCT/CN2016/104215 WO2018081920A1 (zh) 2016-11-01 2016-11-01 基因测序芯片及其安装框

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109985679A (zh) * 2019-04-24 2019-07-09 浙江警察学院 一种spr检测仪用多通道微流控芯片夹持系统
CN112280663A (zh) * 2020-10-29 2021-01-29 王晓冬 一种液滴单层平铺式核酸检测芯片封装件及芯片封装方法

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110387321B (zh) * 2018-04-23 2023-03-17 深圳华大智造科技股份有限公司 基因测序芯片及基因测序装置
WO2019218262A1 (zh) * 2018-05-16 2019-11-21 深圳华大智造科技有限公司 基因测序仪
WO2020034143A1 (zh) * 2018-08-16 2020-02-20 深圳华大智造科技有限公司 手柄装置、定位装置、加载装置及基因测序仪
CN108913590A (zh) * 2018-09-07 2018-11-30 崔化先 一种基因测序芯片及制作方法
US11583856B2 (en) * 2019-04-02 2023-02-21 Beijing Boe Optoelectronics Technology Co., Ltd. Bio-information detection substrate and gene chip
CN110004028A (zh) * 2019-04-16 2019-07-12 北京龙基高科生物科技有限公司 一种测序仪用芯片夹持台

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US20100323350A1 (en) * 2007-02-05 2010-12-23 Intelligent Biosystems, Inc. Methods And Devices For Sequencing Nucleic Acids In Smaller Batches
US20140024126A1 (en) * 2012-07-18 2014-01-23 Canon Kabushiki Kaisha Flow-channel device for detecting light emission
CN105013550A (zh) * 2015-07-09 2015-11-04 清华大学深圳研究生院 微流控芯片夹具和微流控芯片
CN105316224A (zh) * 2015-12-07 2016-02-10 中国科学院苏州生物医学工程技术研究所 全自动核酸提取与pcr扩增微流控芯片及其应用方法
CN205368331U (zh) * 2016-01-08 2016-07-06 深圳华大基因研究院 核酸测序芯片
CN105772125A (zh) * 2016-04-23 2016-07-20 北京化工大学 基于3d打印的微流控芯片夹具实验平台

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CN105624020B (zh) * 2014-11-07 2017-11-03 深圳华大基因研究院 用于检测dna片段的碱基序列的微流控芯片

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100323350A1 (en) * 2007-02-05 2010-12-23 Intelligent Biosystems, Inc. Methods And Devices For Sequencing Nucleic Acids In Smaller Batches
US20140024126A1 (en) * 2012-07-18 2014-01-23 Canon Kabushiki Kaisha Flow-channel device for detecting light emission
CN105013550A (zh) * 2015-07-09 2015-11-04 清华大学深圳研究生院 微流控芯片夹具和微流控芯片
CN105316224A (zh) * 2015-12-07 2016-02-10 中国科学院苏州生物医学工程技术研究所 全自动核酸提取与pcr扩增微流控芯片及其应用方法
CN205368331U (zh) * 2016-01-08 2016-07-06 深圳华大基因研究院 核酸测序芯片
CN105772125A (zh) * 2016-04-23 2016-07-20 北京化工大学 基于3d打印的微流控芯片夹具实验平台

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109985679A (zh) * 2019-04-24 2019-07-09 浙江警察学院 一种spr检测仪用多通道微流控芯片夹持系统
CN112280663A (zh) * 2020-10-29 2021-01-29 王晓冬 一种液滴单层平铺式核酸检测芯片封装件及芯片封装方法

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EP3536774B1 (en) 2021-06-16
EP3536774A1 (en) 2019-09-11
CN109328229B (zh) 2021-07-27
EP3536774A4 (en) 2020-08-12
WO2018081920A1 (zh) 2018-05-11

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