CN109319945A - A kind of microbial treatment method and processor of oily waste water - Google Patents

A kind of microbial treatment method and processor of oily waste water Download PDF

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Publication number
CN109319945A
CN109319945A CN201811238054.6A CN201811238054A CN109319945A CN 109319945 A CN109319945 A CN 109319945A CN 201811238054 A CN201811238054 A CN 201811238054A CN 109319945 A CN109319945 A CN 109319945A
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microalgae
waste water
oily waste
reactor
treatment method
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陈庆国
李晶晶
鲍博
刘梅
穆军
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Zhejiang Ocean University ZJOU
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Zhejiang Ocean University ZJOU
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    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00Treatment of water, waste water, or sewage
    • C02F1/40Devices for separating or removing fatty or oily substances or similar floating material
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/32Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
    • C02F3/322Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00Nature of the contaminant
    • C02F2101/30Organic compounds
    • C02F2101/32Hydrocarbons, e.g. oil
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2203/00Apparatus and plants for the biological treatment of water, waste water or sewage
    • C02F2203/006Apparatus and plants for the biological treatment of water, waste water or sewage details of construction, e.g. specially adapted seals, modules, connections
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2305/00Use of specific compounds during water treatment
    • C02F2305/06Nutrients for stimulating the growth of microorganisms

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Water Supply & Treatment (AREA)
  • Hydrology & Water Resources (AREA)
  • Environmental & Geological Engineering (AREA)
  • Microbiology (AREA)
  • Organic Chemistry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a kind of microbial treatment method of oily waste water and processors, the following steps are included: A, preparing the microalgae reactors of several air hoisting types, including bottle body, vent plug, top cover, gas drainage tube, diffuser and injection port, bottle body is protected from light processing;B, microbial identification separation will be carried out from the water sample of oil spill accident collection in worksite, is screened out from it microalgae, the microalgae is carried out enrichment culture 12 ~ 20 days, algae solution is obtained;C, in microalgae reactor of the algae solution for obtaining step B by top injection air hoisting type, microalgae reactor is bisected into several groups, and each microalgae reactor taps into water pipe and aeration tube respectively, realizes sewage sample introduction, stirring diffusion, uniformly mixes with the microalgae algae solution in reactor;After aeration, increase contact of the microalgae with oily waste water, increases microalgae oxygen supply.Benefit of the invention is that the industrial oily waste water that can degrade, microorganism and the air delivery rate in sewage are high, microorganism and sewage mixing rate are fast, and oil removal efficiency is high.

Description

A kind of microbial treatment method and processor of oily waste water
Technical field
The present invention relates to oily waste water biologic treating technique field more particularly to a kind of microbiological treatment sides of oily waste water Method and processor.
Background technique
With the development of industry, the usage amount of oil product is increasing, but not due to the lagging in development of various technologies and management Reasons perfect etc. to the greatest extent make a large amount of oil products enter water body, form pollution.The source of oily waste water is very wide, in adopting for petroleum industry A large amount of oily waste waters can be all generated in oil, oil refining, store oil, transportational process and the production process of petro chemical industry.Oily waste water Pollution be mainly manifested in the following aspects: (1) influence drinking water sources and groundwater resources, endanger aquatic resources;(2) it endangers Evil human health;(3) atmosphere is polluted;(4) production estimation is influenced;(5) natural landscape, or even it is also possible to because coalescence are destroyed Oil product burning and generate safety problem.In view of the pollution of oily waste water, China's regulation oily waste water highest allows to discharge dense Degree is 10mg/L.Therefore oily waste water improvement is current field of environment engineering urgent problem.
For at present, the method for oil removing mainly has chemical method, physical method, biological method, compared with first two processing side Method, bioanalysis is less toxic, without secondary pollution with it, cleans and becomes research emphasis.Microalgae, a master as microorganism treatment Want part, it is widely distributed, small in size, the procreation period is short, the speed of growth is fast.Therefore, select chlorella vulgaris as oily waste water The filling liquid of processing unit.Chlorella vulgaris, as can heterotrophic microalgae, the metabolism that hydrocarbons carry out itself can be efficiently used Activity makes it be degraded to CO2 and H2O, to carry out depth degradation to oily waste water.
For example, application No. is CN201410058175.8 in Chinese patent literature, publication date is open on June 4th, 2014 Entitled " a kind of bioremediation and equipment of oil extraction-generated waste water " patent of invention, this application discloses a kind of oil extraction-generated waste waters Biological treatment, including air-dissolving air-float, the oil removing of microorganism grease removal tank, biological aerated filter filtering, sinterable polymer filter core Filter filters four steps: 1, water enters dissolved air flotation device, removes most of crude oil and suspended matter;2, step 1 go out Water enters microorganism oil removing unit removal crude oil;3, the water outlet of step 2 is promoted to biological aerated filter, and remaining crude oil exists in water It degrades under the action of microorganism, while removing most suspended substances;4, it is fine to be promoted to sinterable polymer filter core for the water outlet of step 3 Filter element, further removes the suspended matter in water removal, and water outlet reaches " oil content≤1mg/L, suspended matter≤1mg/L, median particle size ≤1μm".Disadvantage is that: 1, need to be aerated 20-24 hours for restoring the activity of microbial flora, it is subsequent in order to drop Low aeration rate will also continue bored aeration 40 to 48 hours, long the time required to oil removal process, inefficient;2, oily waste water was aerated Journey, fluid is almost static, and gas exchanges are slow, and the wastewater treatment speed positioned at the corner of biological aerated filter falls behind other positions Waste water needs to increase aeration time to handle the waste water in corner, and waste of resource reduces purification efficiency.
Therefore a kind of design method of the microbiological treatment device of oily waste water is designed to improve oily waste water in oil removal process In transmission efficiency and mixing rate, improve oily waste water degradation rate just it is necessary to.
Summary of the invention
The present invention will overcome the shortcomings of that existing oily waste water oil removal process mixing rate is slow, oil removal efficiency is poor, provide A kind of microbial treatment method and processor of oily waste water, can degrade industrial oily waste water, and microorganism and air are in sewage Middle delivery rate is high, and microorganism and sewage mixing rate are fast, and oil removal efficiency is high.
To achieve the goals above, the present invention adopts the following technical scheme that.
A kind of microbial treatment method of oily waste water, comprising the following steps:
A, prepare the microalgae reactor of several air hoisting types, including bottle body, vent plug, top cover, gas drainage tube, diffuser with Injection port, bottle body are protected from light processing;
B, microbial identification separation will be carried out from the water sample of oil spill accident collection in worksite, microalgae is screened out from it, to the microalgae It carries out enrichment culture 12 ~ 20 days, obtains algae solution;
C, in microalgae reactor of the algae solution for obtaining step B by top injection air hoisting type, if microalgae reactor is bisected into Dry group, each microalgae reactor tap into water pipe and aeration tube respectively, realize sewage sample introduction, stirring diffusion, uniformly and in reactor Microalgae algae solution mixing;After aeration, increase contact of the microalgae with oily waste water, increases microalgae oxygen supply.
While taming microalgae, can efficient degradation hydrocarbons, it is equal in fields such as industrial oily waste water, ship ballast waters It can be used;Step A and step B is preamble work, can be carried out simultaneously with multiple groups, time saving and energy saving, in step C, industrial oil-containing Waste water will enter from the injection port of bottle body bottom, and air-flow is injected into microalgae reactor by the diffuser for being located at bottle body bottom, works as gas When stream enters gas drainage tube, water will be moving upward and be formed upper up-flow, since the gas in gas drainage tube is more anti-than microalgae Answer gas in device more, it is lower in the density of the fluid of microalgae reactor center, because the density contrast generates pressure difference, drive fluid It is recycled in microalgae reactor, low energy consumption, improves the delivery rate of microalgae in sewage, and sewage internal shear power is uniform, mixing speed Rate is fast, improves the efficiency of biological oil removing.
Preferably, microalgae is chlorella in the step B.Chlorella has easily training as the microorganism being widely present It supports, breed the features such as very fast;After domestication, there is efficient petroleum hydrocarbon degradation rate, it can be effectively to the hydrocarbons in water body Carry out degradation chlorella as can heterotrophic microalgae, the metabolic activity that hydrocarbons carry out itself can be efficiently used, make its degradation For CO2 and H2O, to carry out depth degradation to oily waste water.
Preferably, in the step B, enrichment culture are as follows: culture medium is boiled into 10 ~ 20min with seawater, is added after suction filtration Nutritive salt, the concentration of each component is 0.1 ~ 0.3g/L of NH4Cl, 0.05 ~ 0.1g/L of K2HPO4 and citric acid in the nutritive salt 0.005 ~ 0.02g/L of iron.The chlorella crude enzyme liquid for completing the later period extracts experiment and algae passage.
Preferably, in the step B, enrichment culture are as follows: culture medium is boiled into 12 ~ 18min with seawater, is to mend after suction filtration The necessary nutriment of chlorella growth is filled, corresponding nutritive salt is added, addition NH4Cl is supplementing for supplement nitrogen source, K2HPO4 The addition of Element Potassium, ironic citrate of can supplementing the nutrients while phosphorus source can then meet chlorella during the growth process to ferro element Needs, the concentration of each component is respectively NH4Cl 0.2g/L, K2HPO4 0.05g/L and ironic citrate in three kinds of nutritive salt 0.01g/L.The chlorella crude enzyme liquid for completing the later period extracts experiment and algae passage.
Preferably, chlorella incubation time is 18 days in the step B.Obtain enough chlorellas.
Preferably, microalgae reactor volume is 3L in the step C.
Preferably, the microalgae algae solution volume of injection is 1L in the step C.
Preferably, sewage sampling volume is 1L in the step C.
Preferably, in the step C, aeration time 12h.For the gas drainage tube in microalgae reactor when aeration Caused by draught head can promote contact of the microalgae with oily waste water, increase microalgae oxygen supply, oil removal efficiency improved, so only needing 12 Hour, it is time-consuming short.
A kind of microbiological treatment device applied to the above method, including bottle body, bottle body upper end are equipped with top cover, and bottle body lower end is set There are the injection port into sewage and the diffuser into air, bottle body upper end is fixed with gas drainage tube, the connection of gas drainage tube Inside and outside bottle body, the upper end of gas drainage rolling is equipped with vent plug, and gas drainage tube is opposite with diffuser.
Therefore, the invention has the following beneficial effects:
1, chlorella is as the microorganism being widely present, have the characteristics that easily to cultivate, breed it is very fast, in the present invention chlorella be from Oil spill accident collection in worksite, separation, culture and obtain, by domestication after, have efficient petroleum hydrocarbon degradation rate, can be effectively to water body In hydrocarbons degrade;
2, it is mostly physical treatment method and chemical treatment method currently used for the processing of industrial oily waste water, exists secondary to environment The potential risk of pollution selects the algae solution of chlorella vulgaris to carry out advanced treating, more cleans, is environmentally friendly;
3, microalgae and oily waste water can be promoted for draught head caused by the gas drainage tube in microalgae reactor when being aerated Contact, increases microalgae oxygen supply, and oil removal efficiency is high;
4, the microalgae in the design tames system, can carry out factor adjusting, strong flexibility, and the body according to different actual conditions Be it is small in size, occupied area is small, can be applied to the subsequent processing of ship ballast water etc., has wide range of applications, and has preferable reality Application prospect.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of microalgae reactor in the present invention.
Fig. 2 is a kind of structural schematic diagram of preferred microalgae domestication system in the present invention.
In figure: lower 12 vent plug of sealing plate, 2 top cover, 3 gas drainage tube, 4 diffuser 5 of 1 upper sealing plate 11 of bottle body 6 air blower of injection port, 7 fluid stirrer 8.
Specific embodiment
The present invention is described further in the following with reference to the drawings and specific embodiments.
Fig. 1 is the structure chart of microalgae reactor.Microalgae reactor be closing or semi-enclosed biomass production system, can To provide stable and reliable environment for algal grown, while reducing external contamination.Microalgae reactor includes cylindrical bottle body 1,1 upper end of bottle body is equipped with top cover 3, and 1 lower end of bottle body is equipped with the injection port 6 into sewage and the diffuser 5 into air, bottle body 1 Upper end is fixed with gas drainage tube 4, and gas drainage tube 4 is connected to inside and outside bottle body 1, and the upper end of gas drainage rolling is equipped with vent plug 2, gas Drainage tube 4 and diffuser 5 are opposite, and the upper end of bottle body 1 is equipped with upper sealing plate 11, and the lower end of bottle body 1 is equipped with lower sealing plate 12, gas drainage Pipe 4 and top cover 3 are fixed on upper sealing plate 11, and injection port 6 and diffuser 5 are located on lower sealing plate 12, upper sealing plate 11 and lower sealing plate 12 It can be screwed and connect with bottle body 1, facilitate the assembly and replacement of each component of microalgae reactor.In view of the oily waste water of high concentration Adhesiveness, the super-hydrophobic nano-coating material of bottle body 1 inner-wall spraying, one layer of physics of microalgae reactor, and in 1 outer wall of bottle body into The shading treatment of row black spraying, is in the microalgae in reactor under the conditions of heterotrophism.The structure of microalgae reactor is simple, cost It is cheap, it can also realize very tiny gas diffusion and mixing, consume energy low.Microalgae reactor be low energy consumption, delivery rate is high, The biomass production system that shearing force is uniform, mixing rate is fast and Land Demand is few.
In Fig. 1, industrial oily waste water will enter from the injection port 6 of 1 bottom of bottle body, and air-flow is by the expansion positioned at 1 bottom of bottle body It dissipates device 5 and is injected into microalgae reactor, when air-flow enters gas drainage tube 4, water will be moving upward and be formed upper up-flow, diffusion Device 5 is located at the middle position of 1 lower part of bottle body, and gas drainage tube 4 also is located at microalgae inside reactor centered position, due to gas Gas in drainage tube 4 is more than gas in microalgae reactor, lower in the density of the fluid of microalgae reactor center, because this is close Degree difference generates pressure difference, and fluid is driven to recycle in microalgae reactor.
Fig. 2 is a kind of preferred microalgae domestication system schematic.In Fig. 2, the culture systems of domestication are induced by 10 microvesicles Microalgae reactor group tap into water pipe and aeration tube respectively at, each microalgae reactor, water inlet pipe connects a fluid stirrer 8, Aeration tube connect an air blower 7, be shown in Fig. 2 one it is small-scale integrate microalgae domestication, oil spilling handle microorganism it is anti- Device is answered, microbubble technique can realize higher air mass transfer rate, mix gas-liquid preferably, to reach higher micro algae growth Rate, and then improve the efficiency of domestication system.
A kind of microbial treatment method of oily waste water, comprising the following steps:
A, prepare the microalgae reactor of several air hoisting types, microalgae reactor volume is 3L, including bottle body 1, vent plug 2, top Lid 3, gas drainage tube 4, diffuser 5 and injection port 6, bottle body 1 are protected from light processing, realize the no light conditions of micro algae growth, and confession is micro- Algae carries out the depth degradation of heterotrophism to the petroleum hydrocarbon in waste water;
B, microbial identification separation will be carried out from the water sample of oil spill accident collection in worksite, is screened out from it microalgae, microalgae is bead Algae.The microalgae is carried out enrichment culture 12 ~ 20 days, preferably 18 days;Enrichment culture are as follows: culture medium is boiled 10 with seawater ~ 20min adds nutritive salt after suction filtration, and the concentration of each component is 0.1 ~ 0.3g/L of NH4Cl, K2HPO4 0.05 in the nutritive salt 0.005 ~ 0.02g/L of ~ 0.1g/L and ironic citrate.Obtain algae solution;
C, the algae solution for obtaining step B is promoted in microalgae reactor by top injection air, and the microalgae algae solution volume of injection is 1L; Every 5 reactors are one group, totally two groups.Each microalgae reactor taps into water pipe and aeration tube respectively, realizes sewage sample introduction, sewage Sampling volume is 1L, and stirring diffusion is uniformly mixed with the microalgae algae solution in reactor.Aeration time is 12h, after aeration, is increased Contact of the microalgae with oily waste water increases microalgae oxygen supply.
Chlorella crude enzyme liquid to carry out the later period extracts experiment and algae passage, carries out the enrichment culture of chlorella.It is described Enrichment culture are as follows: culture medium is boiled into 12 ~ 18min with seawater, is the supplement necessary nutriment of chlorella growth after suction filtration, adds Add corresponding nutritive salt, addition NH4Cl be supplement nitrogen source, K2HPO4 can supplement the nutrients while supplementing phosphorus source Element Potassium, The addition of ironic citrate can then meet chlorella during the growth process to the needs of ferro element, therefore each group in these three nutritive salt The concentration divided is respectively NH4Cl 0.2g/L, K2HPO4 0.05g/L and ironic citrate 0.01g/L.
In embodiment 1: A, preparing the microalgae reactor of 10 air hoisting types, microalgae reactor volume is 3L, including bottle Body 1, vent plug 2, top cover 3, gas drainage tube 4, diffuser 5 and injection port 6.Bottle body 1 is protected from light processing, realizes the nothing of micro algae growth Luminous environment carries out the depth degradation of heterotrophism for microalgae to the petroleum hydrocarbon in waste water.
B, microbial identification separation will be carried out from the water sample of oil spill accident collection in worksite, is screened out from it microalgae, microalgae is Chlorella.The microalgae is carried out enrichment culture 15 days, culture medium is boiled into 12 ~ 18min with seawater, is added after suction filtration corresponding Nutritive salt, the concentration of each component is respectively NH4Cl 0.2g/L, K2HPO4 0.05g/L and ironic citrate in three kinds of nutritive salt 0.01g/L obtains algae solution.
The algae solution that C obtains step B is promoted in microalgae reactor by top injection air, and the microalgae algae solution volume of injection is 1L;Every 5 reactors are one group, totally two groups.Each microalgae reactor taps into water pipe and aeration tube respectively, realizes sewage sample introduction, Sewage sampling volume is 1L, and stirring diffusion is uniformly mixed with the microalgae algae solution in reactor.Aeration time is 12h, after aeration, Increase contact of the microalgae with oily waste water, increases microalgae oxygen supply.
In embodiment 2:
A, prepare 6 air hoisting types microalgae reactor, microalgae reactor volume be 3L, including bottle body 1, vent plug 2, top cover 3, Gas drainage tube 4, diffuser 5 and injection port 6.Bottle body 1 is protected from light processing, the no light conditions of micro algae growth is realized, for microalgae pair Petroleum hydrocarbon in waste water carries out the depth degradation of heterotrophism.
B, microbial identification separation will be carried out from the water sample of oil spill accident collection in worksite, microalgae is screened out from it, to described Microalgae carries out enrichment culture 18 days, and culture medium is boiled 12 ~ 18min with seawater, and corresponding nutritive salt, three kinds of battalion are added after suction filtration The concentration for supporting each component in salt is respectively NH4Cl 0.2g/L, K2HPO4 0.05g/L and ironic citrate 0.01g/L, obtains algae Liquid.
C, the algae solution for obtaining step B is promoted in microalgae reactor by top injection air, the microalgae algae solution volume of injection For 1L;Every 3 reactors are one group, totally two groups;Each microalgae reactor taps into water pipe and aeration tube respectively, realize sewage into Sample, sewage sampling volume are 1L, and stirring diffusion is uniformly mixed with the microalgae algae solution in reactor;Aeration time is 12h, aeration Afterwards, increase contact of the microalgae with oily waste water, increase microalgae oxygen supply.
In embodiment 3: A, preparing the microalgae reactor of 10 air hoisting types, microalgae reactor volume is 3L, including bottle Body 1, vent plug 2, top cover 3, gas drainage tube 4, diffuser 5 and injection port 6;Bottle body 1 is protected from light processing, realizes the nothing of micro algae growth Luminous environment carries out the depth degradation of heterotrophism for microalgae to the petroleum hydrocarbon in waste water.
B, microbial identification separation will be carried out from the water sample of oil spill accident collection in worksite, microalgae is screened out from it, to described Microalgae carries out enrichment culture 18 days, and culture medium is boiled 12 ~ 18min with seawater, and corresponding nutritive salt, three kinds of battalion are added after suction filtration The concentration for supporting each component in salt is respectively NH4Cl 0.2g/L, K2HPO4 0.05g/L and ironic citrate 0.01g/L, obtains algae Liquid.
C, the algae solution for obtaining step B is promoted in microalgae reactor by top injection air, the microalgae algae solution volume of injection For 1L;Every 5 reactors are one group, totally 2 groups;Each microalgae reactor taps into water pipe and aeration tube respectively, realizes sewage sample introduction, Sewage sampling volume is 1L, and stirring diffusion is uniformly mixed with the microalgae algae solution in reactor;Aeration time is 12h, after aeration, Increase contact of the microalgae with oily waste water, increases microalgae oxygen supply.
Embodiment 1-3 is surveyed through the microalgae domestication processed oily waste water of system (3g/L) using spectrophotometry Fixed: measurement result is as shown in table 1.
1. measurement result of table
Performance indicator Embodiment 1 Embodiment 2 Embodiment 3
Oil degradation rate (%) 88.63 90.52 98.99
As can be seen from Table 1, the present invention a kind of the microbiological treatment device and processing method of oily waste water, can be effectively to oily waste water Depth degradation is carried out, and degradation efficiency is relatively high.This shows that in actual application the reaction system can be effective It degraded, removed to hydrocarbons.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the present invention in any form, and is not surpassing There are also other variations and modifications under the premise of technical solution documented by claim out.

Claims (10)

1. a kind of microbial treatment method of oily waste water, characterized in that the following steps are included:
A, prepare the microalgae reactor of several air hoisting types, including bottle body, vent plug, top cover, gas drainage tube, diffuser with Injection port, bottle body are protected from light processing;
B, microbial identification separation will be carried out from the water sample of oil spill accident collection in worksite, microalgae is screened out from it, to the microalgae It carries out enrichment culture 12 ~ 20 days, obtains algae solution;
C, in microalgae reactor of the algae solution for obtaining step B by top injection air hoisting type, if microalgae reactor is bisected into Dry group, each microalgae reactor tap into water pipe and aeration tube respectively, realize sewage sample introduction, stirring diffusion, uniformly and in reactor Microalgae algae solution mixing;After aeration, increase contact of the microalgae with oily waste water, increases microalgae oxygen supply.
2. a kind of microbial treatment method of oily waste water according to claim 1, characterized in that micro- in the step B Algae is chlorella.
3. a kind of microbial treatment method of oily waste water according to claim 1 or 2, characterized in that the step B In, the enrichment culture are as follows: culture medium is boiled into 10 ~ 20min with seawater, adds nutritive salt after suction filtration, it is each in the nutritive salt The concentration of component is 0.1 ~ 0.3g/L of NH4Cl, 0.005 ~ 0.02g/L of 0.05 ~ 0.1g/L of K2HPO4 and ironic citrate.
4. a kind of microbial treatment method of oily waste water according to claim 3, characterized in that in the step B, institute State enrichment culture are as follows: culture medium is boiled into 12 ~ 18min with seawater, is the supplement necessary nutriment of chlorella growth after suction filtration, Add corresponding nutritive salt, addition NH4Cl is that supplement nitrogen source, K2HPO4 can supplement the nutrients element while supplementing phosphorus source The addition of potassium, ironic citrate can then meet chlorella during the growth process to the needs of ferro element, each component in three kinds of nutritive salt Concentration be respectively NH4Cl 0.2g/L, K2HPO4 0.05g/L and ironic citrate 0.01g/L.
5. a kind of microbial treatment method of oily waste water according to claim 2, characterized in that small in the step B Ball algae incubation time is 18 days.
6. a kind of microbial treatment method of oily waste water according to claim 2, characterized in that micro- in the step C Algae reactor volume is 3L.
7. a kind of microbial treatment method of oily waste water according to claim 6, characterized in that in the step C, note The microalgae algae solution volume entered is 1L.
8. a kind of microbial treatment method of oily waste water according to claim 6 or 7, characterized in that the step C In, sewage sampling volume is 1L.
9. a kind of microbial treatment method of oily waste water described according to claim 1 or 2 or 6, characterized in that the step In C, aeration time 12h.
10. a kind of processor of the microbial treatment method applied to the oily waste water described in claim 1 to 9, characterized in that Including bottle body, bottle body upper end is equipped with top cover, and bottle body lower end is equipped with the injection port into sewage and the diffuser into air, bottle body Upper end is fixed with gas drainage tube, gas drainage tube air expansion bottle inside and outside, and the upper end of gas drainage rolling is equipped with vent plug, gas drainage It manages opposite with diffuser.
CN201811238054.6A 2018-10-23 2018-10-23 A kind of microbial treatment method and processor of oily waste water Pending CN109319945A (en)

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Application publication date: 20190212