CN109312379B - 多功能重组核苷酸依赖性糖基转移酶蛋白及其糖基化的方法 - Google Patents

多功能重组核苷酸依赖性糖基转移酶蛋白及其糖基化的方法 Download PDF

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CN109312379B
CN109312379B CN201780031562.4A CN201780031562A CN109312379B CN 109312379 B CN109312379 B CN 109312379B CN 201780031562 A CN201780031562 A CN 201780031562A CN 109312379 B CN109312379 B CN 109312379B
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seq
sugar
glycosylation
product
diglycosylated
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CN109312379A (zh
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A·饶
R·纳加尔
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Council of Scientific and Industrial Research CSIR
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)

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  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201780031562.4A 2016-04-05 2017-03-30 多功能重组核苷酸依赖性糖基转移酶蛋白及其糖基化的方法 Expired - Fee Related CN109312379B (zh)

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IN201611011974 2016-04-05
IN201611011974A IN201611011974A (https=) 2016-04-05 2017-03-30
PCT/IN2017/050116 WO2017175239A1 (en) 2016-04-05 2017-03-30 A multifunctional recombinant nucleotide dependent glycosyltransferase protein and its method of glycosylation thereof

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CN109312379B true CN109312379B (zh) 2022-04-12

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US (1) US11485991B2 (https=)
EP (1) EP3423587B1 (https=)
CN (1) CN109312379B (https=)
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WO (1) WO2017175239A1 (https=)

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EP3844271A4 (en) * 2018-08-29 2022-06-01 Council of Scientific and Industrial Research Recombinant microbial system for directed evolution of glycocins and a method of preparation thereof
US20220228186A1 (en) * 2019-05-23 2022-07-21 Arzeda Corp. Compositions and methods for producing steviol glycosides

Citations (3)

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CN101151367A (zh) * 2004-02-04 2008-03-26 尼澳斯技术股份有限公司 哺乳动物糖基转移酶的重折叠方法
CN103328630A (zh) * 2011-01-20 2013-09-25 詹尼温生物技术有限责任公司 新型岩藻糖基转移酶及其应用
US9333227B2 (en) * 2013-08-19 2016-05-10 Syngulon Sa. Controlled growth of microorganisms

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US6127153A (en) 1995-06-07 2000-10-03 Neose Technologies, Inc. Method of transferring at least two saccharide units with a polyglycosyltransferase, a polyglycosyltransferase and gene encoding a polyglycosyltransferase
EP1066388A2 (de) 1998-03-25 2001-01-10 GVS Gesellschaft für Erwerb und Verwertung Landwirtschaftlicher Pflanzensorten MBH Prozessive glycosyltransferase
AU6059699A (en) 1998-09-23 2000-04-10 Regents Of The University Of California, The Synthetic peptides, conjugation reagents and methods
WO2002000851A2 (en) 2000-06-28 2002-01-03 National Research Council Of Canada Helicobacter dd-heptosyltransferase
ATE506450T1 (de) 2002-07-23 2011-05-15 Biogenerix Ag Synthese von oligosacchariden, glykolipiden und glykoproteinen unter verwendung bakterieller glykosyltransferasen
NZ548123A (en) 2004-01-08 2010-05-28 Novo Nordisk As O-linked glycosylation of peptides
EP1981977B1 (en) 2006-01-31 2015-12-23 National Research Council Of Canada Production of polysialic acid containing glyconjugates using a self-priming polysialyltransferase
EP2049144B8 (en) 2006-07-21 2015-02-18 ratiopharm GmbH Glycosylation of peptides via o-linked glycosylation sequences
WO2008151258A2 (en) 2007-06-04 2008-12-11 Neose Technologies, Inc. O-linked glycosylation using n-acetylglucosaminyl transferases
CN101983070B (zh) 2008-02-20 2016-03-30 格林考瓦因有限公司 由来源于原核细胞的重组n-糖基化蛋白制备生物共轭物
FR2954138B1 (fr) 2009-12-17 2012-03-02 Oreal Procede cosmetique de traitement des odeurs corporelles utilisant une composition a base de bacteriocines
JP5936553B2 (ja) 2011-01-14 2016-06-22 サントリーホールディングス株式会社 新規糖転移酵素遺伝子及びその使用
CN102389030B (zh) 2011-09-16 2013-12-25 李叶青 枯草杆菌糖肽素168的用途
RU2620070C2 (ru) 2011-12-13 2017-05-22 Сантр Насьональ Де Ля Решерш Сьянтифик Модифицированные пептиды и их применение для лечения аутоиммунных заболеваний

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Publication number Priority date Publication date Assignee Title
CN101151367A (zh) * 2004-02-04 2008-03-26 尼澳斯技术股份有限公司 哺乳动物糖基转移酶的重折叠方法
CN103328630A (zh) * 2011-01-20 2013-09-25 詹尼温生物技术有限责任公司 新型岩藻糖基转移酶及其应用
US9333227B2 (en) * 2013-08-19 2016-05-10 Syngulon Sa. Controlled growth of microorganisms

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
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Enterococcus faecalis V583 plasmid pTEF2, complete sequence;Paulsen I.等;《DATABASE EMBL》;20030329;第32页Sequence部分 *
Features and applications of bacterial glycosyltransferases: current state and prospects;Andriy Luzhetskyy等;《Appl Microbiol Biotechnol》;20080906;第80卷(第6期);全文 *
glycosyltransferase family 2 protein [Enterococcus faecalis];佚名;《GenBank》;20130526;第1页ORIGIN部分 *

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Publication number Publication date
WO2017175239A1 (en) 2017-10-12
EP3423587A1 (en) 2019-01-09
CN109312379A (zh) 2019-02-05
IN201611011974A (https=) 2018-02-02
US11485991B2 (en) 2022-11-01
EP3423587B1 (en) 2020-06-24
WO2017175239A4 (en) 2017-12-28
US20210071221A1 (en) 2021-03-11

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