CN109287381A - A kind of Hericium erinaceus culture substrate and the method using the substrate fermentation culture Hericium erinaceus mycoplasma - Google Patents
A kind of Hericium erinaceus culture substrate and the method using the substrate fermentation culture Hericium erinaceus mycoplasma Download PDFInfo
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- CN109287381A CN109287381A CN201811452479.7A CN201811452479A CN109287381A CN 109287381 A CN109287381 A CN 109287381A CN 201811452479 A CN201811452479 A CN 201811452479A CN 109287381 A CN109287381 A CN 109287381A
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- hericium erinaceus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a kind of Hericium erinaceus culture substrate and use the method for the substrate fermentation culture Hericium erinaceus mycoplasma, for the Hericium erinaceus mycoplasma of solid ferment culture high quality, the Hericium erinaceus Polysaccharides of high-content, scheme selects edible fungi original seed to produce, and traditional materials-wheat, nutritive value is high and has effects that the quick-acting carbon source edible glucoses that the frying seed of jog's tears of invigorating the spleen to arrest diarrhea, full of nutrition and C/N ratio are well suited for the wheat bran of Hericium erinaceus nutrient growth and can quickly be utilized by Hericium erinaceus, weight ratio of constituents are as follows: the seed of jog's tears and or 76~88 parts of wheat, 10~20 parts of wheat bran, 2~4 parts of glucose.In the present invention, Hericium erinaceus mycelia grow faster, it is growing way Geng Wang, denseer white, the Hericium erinaceus mycoplasma nutrition of cultivation and functional component closer to fructification, energy conservation, drop this, pure natural 0 addition of product.
Description
Technical field
It is trained the present invention relates to a kind of matrix and using the method for the substrate fermentation culture mycoplasma more particularly to a kind of Hericium erinaceus
Support matrix and the method using the substrate fermentation culture Hericium erinaceus mycoplasma.
Background technique
Hericium erinaceus is the rare dual-purpose of drug and food bacterium of a kind of modern medicine and pharmacology confirmation.In the 1960s, state
Interior pharmacy science and technology worker uses for reference ancient times " yeast-making technology " use " solid culture " mode and cultivates hedgehog hydnum with broken bagasse, wheat bran etc.
Bacterium about 40d generates a large amount of mycelium, obtains " hedgehog fungus mycelium culture ", dug out bottle it is dry after as medicinal material, then through at
Divide and extract, medicinal extract is concentrated to give after dregs of a decoction filtering removal, Hericium erinaceus drug is made, but because it needs effective component extracting and discard to train
Supporting invalid " mushroom bran " that matrix generates could apply and endure dispute to the fullest extent.The seventies and eighties, through domestication, with sawdust, cotton seed hull
Cultivation and Hericium erinaceus liquid submerged fermentation succeed in succession.But artificial cultivation obtain fructification process it is various, the period is very long, at
This is higher, is also limited and is influenced by many natures and planting environment condition；Although liquid fermentation produces mycelium and cultivates the time
Short, mycelia can control with polysaccharide yield, and not influenced by outside environmental elements, but its working condition requirement of guaranteeing both quality and quantity is severe
It carves, is easy to appear pollution, investment is big, high production cost, and liquid environment causes many important physiological activators can not
It generates, mycelium and fructification is caused to form difference in terms of effect.Develop into solid fermentation production from the solid culture eighties
Since fungi-medicine, using solid fermentation production mycoplasma be healthy food, functional food or medical product provide raw material increasingly by
It is researched and developed to industry and payes attention to and apply.Open riverine wait paddy in the medicinal function of Hericium erinaceus and its making and research of mycelia medicinal granules of powder
The raw materials such as grain, millet, wheat, maize pulp are eluriated through impurity elimination, are impregnated, boiling, and sunning adds edible plaster, mixes thoroughly, pack (or specially
With seed bottle), sterilizing connects liquid spawn (or connect grain of rice strain and be compacted), temperature and humidity control shading bacteria, and purseful supports 7~10 again
It, is dried for standby.Test proves that the raw materials such as single grain, millet, wheat, maize pulp add edible plaster culture Hericium erinaceus mycelia raw
Long speed is slower, and density is sparse, growing way is weaker, and content mycoplasma is seldom.Trace it to its cause may be that culture medium impregnates, sterilizes
The subalimentations such as the carbon nitrogen quickly utilized for mycelia early growth period are oozed out, only after hyphal cell generation enzyme decomposition solid matrix
It can just absorb nourishment, growing mycelia, and smaller using the intergranular pore that single-matrix is formed, to affect Hericium erinaceus bacterium
Stand density, speed and the growing way of silk.The second is base starting material need to heat boiling, also to be dried after boiling, this not only trouble but also increase
The amount of labour used, which also consumes energy, is unfavorable for large-scale production.Thirdly being exactly that the energy consumption of temperature and humidity control bacteria is high, at high cost.Fourth is that purseful is supported again
Baking in 7~10 days is with whether optimal selection is still to be tested.
Summary of the invention
Exempt to boil it is an object of the invention to overcome the deficiencies of the prior art and provide one kind, exempt from sunning, mycoplasma mycelium and secondary
Give birth to metabolite content height, the Hericium erinaceus culture substrate of low energy consumption and using the substrate fermentation culture Hericium erinaceus mycoplasma
Method.
The purpose of the present invention is achieved through the following technical solutions:
A kind of Hericium erinaceus culture substrate, the ingredient including following parts by weight:
The seed of jog's tears and or 76~88 parts of wheat；
10~20 parts of wheat bran；
2~4 parts of glucose.
Preferably, the seed of jog's tears is the frying seed of jog's tears.
Preferably, the glucose is edible glucose.
Preferably, Hericium erinaceus culture substrate further includes frying Chinese yam, frying Semen Lablab Album, Poria cocos and lotus seeds.
A method of using Hericium erinaceus culture substrate fermented and cultured Hericium erinaceus mycoplasma, comprising the following steps:
S1, Hericium erinaceus culture substrate preparation the following steps are included:
S11, immersion, using the seed of jog's tears and or wheat as major ingredient, and by weight for major ingredient: water=1.0: 1.5~2.0 put major ingredient
Enter and impregnated in 100 DEG C of water, stops impregnating when weight reaches 170~180 kilograms after the water suction of every double centner major ingredient, so
Afterwards by major ingredient cleaning, pull out and filter to water flow at pouring into spice container after drop；
S12, spice, bottling pour into the spice container for filling major ingredient after mixing wheat bran and glucose, and Hericium erinaceus are cultivated
The water content of matrix controls between 42 ~ 46%, is then charged into tissue culture bottle, lid band film breather hole cover；
S13, sterilizing carry out high pressure with Hericium erinaceus culture substrate of the 0.15~0.16MPa to water content 42 ~ 46% in tissue culture bottle and go out
Bacterium；
S2, Hericium erinaceus mycoplasma fermented and cultured the following steps are included:
S21, inoculation, when temperature in tissue culture bottle is lower than 30 DEG C, aseptically first by Hericium erinaceus culture substrate in tissue culture bottle
Shaking is loose, then accesses the wheat original seed through shaking flask culture；
Tissue culture bottle after inoculation, is moved in culturing room by S22, fermented and cultured, and shading stands incubated at room temperature, room temperature 6~30 DEG C it
It is interior, after Hericium erinaceus mycelia covers with matrix in tissue culture bottle, then use 2~3 weeks (rooms of dim light spontaneous fermentation culture of 50~400lx
Warm height determines incubation time length)；
S23, drying open tissue culture bottle and take out Hericium erinaceus mycoplasma obtained by fermented and cultured, mycoplasma is scattered and is dried for standby.
Preferably, step S11 further includes a major ingredient pre-treatment step: using the seed of jog's tears and or wheat as major ingredient be put into Sheng
There is the container of proper amount of clear water, stirs 3~5 minutes, pick up showy impurity, filter out major ingredient, the major ingredient filtered out is put into cool-bag
In.
Preferably, the tissue culture bottle is the plant tissue culture bottle of 380ml, is packed into 130 ± 1g monkey in each plant tissue culture bottle
Head bacterium culture medium matter.
Preferably, a length of 2h when sterilizing in step S13.
Preferably, it is dried in step S23 using baking oven, drying temperature is 50~60 DEG C.
Preferably, the wheat original seed grain number accessed in each tissue culture bottle in step S21 is identical.
The beneficial effects of the present invention are:
1) this Hericium erinaceus culture substrate is other than the nutriments such as the wheat selected with conventional medium, and also proportion joined C/N
Than the wheat bran and quick-acting carbon source edible glucoses that are well suited for Hericium erinaceus nutrient growth so that the growth of Hericium erinaceus mycelia faster, growing way
It is Geng Wang, denseer white, it is high and have effects that the frying seed of jog's tears of invigorating the spleen to arrest diarrhea, the Hericium erinaceus bacterium turned out to have additionally incorporated nutritive value
Matter quality is high, and polyoses content is high.
2) the Hericium erinaceus mycoplasma nutrition cultivated and functional component are closer to fructification: in Hericium erinaceus culture substrate, hedgehog hydnum
Bacterium is decomposed and is absorbed under the action of cell producing enzyme, to Hericium erinaceus its nutrition, grows itself thallus, while can be generated a variety of
Secondary metabolites with functional component.Moreover, because low light culture is conducive to promote Hericium erinaceus reproductive growth after mycelia expires bottle
" mycelium " for forming class fructification, to improve mycoplasma functional component content.
3) energy conservation, drop this: since fermentation bacteria is self-supporting using humidity in bottle, as long as and fermentation bacteria temperature is nature room
Temperature does not just have to control temperature and humidity, therefore save electric energy, reduces labor management expense, to reduce within 6~30 DEG C
Production cost.
4) pure natural 0 addition of product: the raw material for selection of fermenting is all edible food materials, does not add any food
Additive and inorganic compound, fermentation bacteria, which is also in the plant tissue culture bottle of relative closure, to carry out, so production Product Green
Naturally, clean hygiene can be used as the raw materials for production of healthy food, functional food or drug.
Specific embodiment
Below in conjunction with embodiment, technical solution of the present invention is clearly and completely described, it is clear that described
Embodiment is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field
Technical staff's every other embodiment obtained under the premise of not making the creative labor belongs to what the present invention protected
Range.
The present invention provides six kinds of technical solutions:
Embodiment one:
A kind of Hericium erinaceus culture substrate, the ingredient including following parts by weight:
76 parts of the frying seed of jog's tears；
20 parts of wheat bran；
4 parts of edible glucose.
A method of using the Hericium erinaceus culture substrate fermented and cultured Hericium erinaceus mycoplasma, comprising the following steps:
S1, Hericium erinaceus culture substrate preparation the following steps are included:
S11, immersion are put into cool-bag using the high-quality frying seed of jog's tears as major ingredient, and are by weight major ingredient: water=1.0: 2.0
It is impregnated being added in cool-bag in 100 DEG C of water, when major ingredient is steeped to abundant water suction, every double centner major ingredient is heavy after absorbing water
Stop impregnating when amount reaches 178 kilograms, then by major ingredient cleaning, pull out and filter to water flow at pouring into spice container after drop；
S12, spice, bottling are poured into the spice container for filling major ingredient, are converted by weighing after mixing wheat bran and glucose, will
The water content of Hericium erinaceus culture substrate is mixed thoroughly after being adjusted to 44%, is then charged into tissue culture bottle, lid band film breather hole cover, thus
It can promote gas exchanges inside and outside bottle, meet aerobic Hericium erinaceus mycelia growth, the method not only can control water content of substrate, but also can subtract
The boiling of few process and the big raw material of the amount of avoiding is inconvenient；
S13, sterilizing carry out high pressure sterilization 2h with Hericium erinaceus culture substrate of the 0.15MPa to water content 44% in tissue culture bottle；
S2, Hericium erinaceus mycoplasma fermented and cultured the following steps are included:
S21, inoculation aseptically first will be in tissue culture bottles when Hericium erinaceus culture substrate temperature in tissue culture bottle is lower than 30 DEG C
The shaking of Hericium erinaceus culture substrate is loose, then accesses the wheat original seed through shaking flask culture, this operation is sought to aerobic Hericium erinaceus mycelia
Good growth gap has been made, has been conducive to mycelia and grows inside the base, has increased mycoplasma hyphae content；
Tissue culture bottle after inoculation, is moved in culturing room by S22, fermented and cultured, and shading stands incubated at room temperature, and room temperature is 6 DEG C, works as hedgehog hydnum
Bacterium mycelia is covered in tissue culture bottle after matrix, then uses dim light spontaneous fermentation culture 3 weeks of 100lx, this bacteria mode neither shadow
Ring the growth of Hericium erinaceus mycelia, moreover it is possible to energy consumption be greatly lowered, it may be more desirable to which later period low light culture can also obtain class
The Hericium erinaceus " mycelium " of entity；
S23, drying open tissue culture bottle and take out culture gained Hericium erinaceus mycoplasma after fermentation, mycoplasma is scattered and baking oven is used to carry out
Drying, drying temperature are 50 DEG C.
Further, step S11 further includes a major ingredient pre-treatment step: poured into using the frying seed of jog's tears as major ingredient fill it is suitable
The container of clear water is measured, is stirred 3 minutes, is picked up showy impurity, filter out major ingredient, the major ingredient filtered out is put into cool-bag.
Further, the tissue culture bottle is the plant tissue culture bottle of 380ml, is packed into 131g hedgehog hydnum in each plant tissue culture bottle
Bacterium culture medium matter.
Further, the wheat original seed grain number accessed in each tissue culture bottle in step S21 is identical, to strive for that each bottle mycelia is raw
Length is as consistent as possible, facilitates management.
Embodiment two:
A kind of Hericium erinaceus culture substrate, the ingredient including following parts by weight:
88 parts of wheat；
10 parts of wheat bran；
2 parts of edible glucose.
A method of using the Hericium erinaceus culture substrate fermented and cultured Hericium erinaceus mycoplasma, comprising the following steps:
S1, Hericium erinaceus culture substrate preparation the following steps are included:
S11, immersion, are put into wheat as major ingredient in cool-bag, and by weight for major ingredient: water=1.0: 1.5 by 100 DEG C
Water in be added in cool-bag and impregnated, when major ingredient is steeped to abundant water suction, weight reaches after every double centner major ingredient water suction
Stop impregnating at 170 kilograms, then by major ingredient cleaning, pull out and filter to water flow at pouring into spice container after drop；
S12, spice, bottling are poured into the spice container for filling major ingredient, are converted by weighing after mixing wheat bran and glucose, will
The water content of Hericium erinaceus culture substrate is mixed thoroughly after being adjusted to 42%, is then charged into tissue culture bottle, lid band film breather hole cover, thus
It can promote gas exchanges inside and outside bottle, meet aerobic Hericium erinaceus mycelia growth, the method not only can control water content of substrate, but also can subtract
The boiling of few process and the big raw material of the amount of avoiding is inconvenient；
S13, sterilizing carry out high pressure sterilization 2h with Hericium erinaceus culture substrate of the 0.15MPa to water content 42% in tissue culture bottle；
S2, Hericium erinaceus mycoplasma fermented and cultured the following steps are included:
S21, inoculation aseptically first will be in tissue culture bottles when Hericium erinaceus culture substrate temperature in tissue culture bottle is lower than 30 DEG C
The shaking of Hericium erinaceus culture substrate is loose, then accesses the wheat original seed through shaking flask culture, this operation is sought to aerobic Hericium erinaceus mycelia
Good growth gap has been made, has been conducive to mycelia and grows inside the base, has increased mycoplasma hyphae content；
Tissue culture bottle after inoculation, is moved in culturing room by S22, fermented and cultured, and shading stands incubated at room temperature, and room temperature is 18 DEG C, works as hedgehog hydnum
Bacterium mycelia is covered in tissue culture bottle after matrix, then uses dim light spontaneous fermentation culture 18 days of 150lx, this bacteria mode neither shadow
Ring the growth of Hericium erinaceus mycelia, moreover it is possible to energy consumption be greatly lowered, it may be more desirable to which later period low light culture can also obtain class
The Hericium erinaceus " mycelium " of entity；
S23, drying open tissue culture bottle and take out culture gained Hericium erinaceus mycoplasma after fermentation, mycoplasma is scattered and baking oven is used to carry out
Drying, drying temperature are 60 DEG C.
Further, step S11 further includes a major ingredient pre-treatment step: pouring into and is filled in right amount clearly using wheat as major ingredient
The container of water stirs 5 minutes, picks up showy impurity, filter out major ingredient, the major ingredient filtered out is put into cool-bag.
Further, the tissue culture bottle is the plant tissue culture bottle of 380ml, is packed into 129g hedgehog hydnum in each plant tissue culture bottle
Bacterium culture medium matter.
Further, the wheat original seed grain number accessed in each tissue culture bottle in step S21 is identical, to strive for that each bottle mycelia is raw
Length is as consistent as possible, facilitates management.
Embodiment three:
A kind of Hericium erinaceus culture substrate, the ingredient including following parts by weight:
The frying seed of jog's tears and 84 parts of wheat；
13 parts of wheat bran；
3 parts of edible glucose.
A method of using the Hericium erinaceus culture substrate fermented and cultured Hericium erinaceus mycoplasma, comprising the following steps:
S1, Hericium erinaceus culture substrate preparation the following steps are included:
S11, immersion are put into cool-bag using the high-quality frying seed of jog's tears and wheat as major ingredient, and are by weight major ingredient: water=
To in 100 DEG C of water in addition cool-bag impregnate at 1.0: 1.8, and when major ingredient is steeped to abundant water suction, every double centner major ingredient is inhaled
Stop impregnating when weight reaches 176 kilograms after water, then major ingredient is cleaned, pulled out and filtered to water flow and is held at spice is poured into after drop
Device；
S12, spice, bottling are poured into the spice container for filling major ingredient, are converted by weighing after mixing wheat bran and glucose, will
The water content of Hericium erinaceus culture substrate is mixed thoroughly after being adjusted to 43%, is then charged into tissue culture bottle, lid band film breather hole cover, thus
It can promote gas exchanges inside and outside bottle, meet aerobic Hericium erinaceus mycelia growth, the method not only can control water content of substrate, but also can subtract
The boiling of few process and the big raw material of the amount of avoiding is inconvenient；
S13, sterilizing carry out high pressure sterilization 2h with Hericium erinaceus culture substrate of the 0.16MPa to water content 43% in tissue culture bottle；
S2, Hericium erinaceus mycoplasma fermented and cultured the following steps are included:
S21, inoculation aseptically first will be in tissue culture bottles when Hericium erinaceus culture substrate temperature in tissue culture bottle is lower than 30 DEG C
The shaking of Hericium erinaceus culture substrate is loose, then accesses the wheat original seed through shaking flask culture, this operation is sought to aerobic Hericium erinaceus mycelia
Good growth gap has been made, has been conducive to mycelia and grows inside the base, has increased mycoplasma hyphae content；
Tissue culture bottle after inoculation, is moved in culturing room by S22, fermented and cultured, and shading stands incubated at room temperature, and room temperature is 23 DEG C, works as hedgehog hydnum
Bacterium mycelia is covered in tissue culture bottle after matrix, then uses dim light spontaneous fermentation culture 16 days of 200lx, this bacteria mode neither shadow
Ring the growth of Hericium erinaceus mycelia, moreover it is possible to energy consumption be greatly lowered, it may be more desirable to which later period low light culture can also obtain class
The Hericium erinaceus " mycelium " of entity；
S23, drying open tissue culture bottle and take out culture gained Hericium erinaceus mycoplasma after fermentation, mycoplasma is scattered and baking oven is used to carry out
Drying, drying temperature are 55 DEG C.
Further, step S11 further includes a major ingredient pre-treatment step: being poured into using the frying seed of jog's tears and wheat as major ingredient
The container of proper amount of clear water is filled, is stirred 4 minutes, is picked up showy impurity, filter out major ingredient, the major ingredient filtered out is put into cool-bag
In.
Further, the tissue culture bottle is the plant tissue culture bottle of 380ml, is packed into 130g hedgehog hydnum in each plant tissue culture bottle
Bacterium culture medium matter.
Further, the wheat original seed grain number accessed in each tissue culture bottle in step S21 is identical, to strive for that each bottle mycelia is raw
Length is as consistent as possible, facilitates management.
Example IV:
A kind of Hericium erinaceus culture substrate, the ingredient including following parts by weight:
Frying seed of jog's tears 760g；
Wheat bran 200g；
Edible glucose 40g.
A method of using the Hericium erinaceus culture substrate fermented and cultured Hericium erinaceus mycoplasma, comprising the following steps:
S1, Hericium erinaceus culture substrate preparation the following steps are included:
S11, immersion are put into cool-bag using the high-quality frying seed of jog's tears as major ingredient, and are by weight major ingredient: water=1.0: 2.0
It is impregnated being added in cool-bag in 100 DEG C of water, when major ingredient is steeped to abundant water suction, every double centner major ingredient is heavy after absorbing water
Stop impregnating when amount reaches 178 kilograms, then by major ingredient cleaning, pull out and filter to water flow at pouring into spice container after drop；
S12, spice, bottling are poured into the spice container for filling major ingredient, are converted by weighing after mixing wheat bran and glucose, will
The water content of Hericium erinaceus culture substrate is mixed thoroughly after being adjusted to 44%, is then charged into tissue culture bottle, lid band film breather hole cover, thus
It can promote gas exchanges inside and outside bottle, meet aerobic Hericium erinaceus mycelia growth, the method not only can control water content of substrate, but also can subtract
The boiling of few process and the big raw material of the amount of avoiding is inconvenient；
S13, sterilizing carry out high pressure sterilization 2h with Hericium erinaceus culture substrate of the 0.15MPa to water content 44% in tissue culture bottle；
S2, Hericium erinaceus mycoplasma fermented and cultured the following steps are included:
S21, inoculation aseptically first will be in tissue culture bottles when Hericium erinaceus culture substrate temperature in tissue culture bottle is lower than 30 DEG C
The shaking of Hericium erinaceus culture substrate is loose, then accesses the wheat original seed through shaking flask culture, this operation is sought to aerobic Hericium erinaceus mycelia
Good growth gap has been made, has been conducive to mycelia and grows inside the base, has increased mycoplasma hyphae content；
Tissue culture bottle after inoculation, is moved in culturing room by S22, fermented and cultured, and shading stands incubated at room temperature, and room temperature is 6 DEG C, works as hedgehog hydnum
Bacterium mycelia is covered in tissue culture bottle after matrix, then uses dim light spontaneous fermentation culture 3 weeks of 100lx, this bacteria mode neither shadow
Ring the growth of Hericium erinaceus mycelia, moreover it is possible to energy consumption be greatly lowered, it may be more desirable to which later period low light culture can also obtain class
The Hericium erinaceus " mycelium " of entity；
S23, drying open tissue culture bottle and take out culture gained Hericium erinaceus mycoplasma after fermentation, mycoplasma is scattered and baking oven is used to carry out
Drying, drying temperature are 50 DEG C.
Further, step S11 further includes a major ingredient pre-treatment step: poured into using the frying seed of jog's tears as major ingredient fill it is suitable
The container of clear water is measured, is stirred 3 minutes, is picked up showy impurity, filter out major ingredient, the major ingredient filtered out is put into cool-bag.
Further, the tissue culture bottle is the plant tissue culture bottle of 380ml, is packed into 131g hedgehog hydnum in each plant tissue culture bottle
Bacterium culture medium matter.
Further, the wheat original seed grain number accessed in each tissue culture bottle in step S21 is identical, to strive for that each bottle mycelia is raw
Length is as consistent as possible, facilitates management.
Embodiment five:
A kind of Hericium erinaceus culture substrate, the ingredient including following parts by weight:
Wheat 880g；
Wheat bran 100g；
Edible glucose 20g.
A method of using the Hericium erinaceus culture substrate fermented and cultured Hericium erinaceus mycoplasma, comprising the following steps:
S1, Hericium erinaceus culture substrate preparation the following steps are included:
S11, immersion, are put into wheat as major ingredient in cool-bag, and by weight for major ingredient: water=1.0: 1.5 by 100 DEG C
Water in be added in cool-bag and impregnated, when major ingredient is steeped to abundant water suction, weight reaches after every double centner major ingredient water suction
Stop impregnating at 170 kilograms, then by major ingredient cleaning, pull out and filter to water flow at pouring into spice container after drop；
S12, spice, bottling are poured into the spice container for filling major ingredient, are converted by weighing after mixing wheat bran and glucose, will
The water content of Hericium erinaceus culture substrate is mixed thoroughly after being adjusted to 42%, is then charged into tissue culture bottle, lid band film breather hole cover, thus
It can promote gas exchanges inside and outside bottle, meet aerobic Hericium erinaceus mycelia growth, the method not only can control water content of substrate, but also can subtract
The boiling of few process and the big raw material of the amount of avoiding is inconvenient；
S13, sterilizing carry out high pressure sterilization 2h with Hericium erinaceus culture substrate of the 0.15MPa to water content 42% in tissue culture bottle；
S2, Hericium erinaceus mycoplasma fermented and cultured the following steps are included:
S21, inoculation aseptically first will be in tissue culture bottles when Hericium erinaceus culture substrate temperature in tissue culture bottle is lower than 30 DEG C
The shaking of Hericium erinaceus culture substrate is loose, then accesses the wheat original seed through shaking flask culture, this operation is sought to aerobic Hericium erinaceus mycelia
Good growth gap has been made, has been conducive to mycelia and grows inside the base, has increased mycoplasma hyphae content；
Tissue culture bottle after inoculation, is moved in culturing room by S22, fermented and cultured, and shading stands incubated at room temperature, and room temperature is 18 DEG C, works as hedgehog hydnum
Bacterium mycelia is covered in tissue culture bottle after matrix, then uses dim light spontaneous fermentation culture 18 days of 150lx, this bacteria mode neither shadow
Ring the growth of Hericium erinaceus mycelia, moreover it is possible to energy consumption be greatly lowered, it may be more desirable to which later period low light culture can also obtain class
The Hericium erinaceus " mycelium " of entity；
S23, drying open tissue culture bottle and take out culture gained Hericium erinaceus mycoplasma after fermentation, mycoplasma is scattered and baking oven is used to carry out
Drying, drying temperature are 60 DEG C.
Further, step S11 further includes a major ingredient pre-treatment step: pouring into and is filled in right amount clearly using wheat as major ingredient
The container of water stirs 5 minutes, picks up showy impurity, filter out major ingredient, the major ingredient filtered out is put into cool-bag.
Further, the tissue culture bottle is the plant tissue culture bottle of 380ml, is packed into 129g hedgehog hydnum in each plant tissue culture bottle
Bacterium culture medium matter.
Further, the wheat original seed grain number accessed in each tissue culture bottle in step S21 is identical, to strive for that each bottle mycelia is raw
Length is as consistent as possible, facilitates management.
Embodiment six:
A kind of Hericium erinaceus culture substrate, the ingredient including following parts by weight:
The frying seed of jog's tears and wheat 800g；
Wheat bran 150g；
Edible glucose 35g.
A method of using the Hericium erinaceus culture substrate fermented and cultured Hericium erinaceus mycoplasma, comprising the following steps:
S1, Hericium erinaceus culture substrate preparation the following steps are included:
S11, immersion are put into cool-bag using the high-quality frying seed of jog's tears and wheat as major ingredient, and are by weight major ingredient: water=
To in 100 DEG C of water in addition cool-bag impregnate at 1.0: 1.6, and when major ingredient is steeped to abundant water suction, every double centner major ingredient is inhaled
Stop impregnating when weight reaches 180 kilograms after water, then major ingredient is cleaned, pulled out and filtered to water flow and is held at spice is poured into after drop
Device；
S12, spice, bottling are poured into the spice container for filling major ingredient, are converted by weighing after mixing wheat bran and glucose, will
The water content of Hericium erinaceus culture substrate is mixed thoroughly after being adjusted to 46%, is then charged into tissue culture bottle, lid band film breather hole cover, thus
It can promote gas exchanges inside and outside bottle, meet aerobic Hericium erinaceus mycelia growth, the method not only can control water content of substrate, but also can subtract
The boiling of few process and the big raw material of the amount of avoiding is inconvenient；
S13, sterilizing carry out high pressure sterilization 2h with Hericium erinaceus culture substrate of the 0.15MPa to water content 46% in tissue culture bottle；
S2, Hericium erinaceus mycoplasma fermented and cultured the following steps are included:
S21, inoculation aseptically first will be in tissue culture bottles when Hericium erinaceus culture substrate temperature in tissue culture bottle is lower than 30 DEG C
The shaking of Hericium erinaceus culture substrate is loose, then accesses the wheat original seed through shaking flask culture, this operation is sought to aerobic Hericium erinaceus mycelia
Good growth gap has been made, has been conducive to mycelia and grows inside the base, has increased mycoplasma hyphae content；
Tissue culture bottle after inoculation, is moved in culturing room by S22, fermented and cultured, and shading stands incubated at room temperature, and room temperature is 30 DEG C, works as hedgehog hydnum
Bacterium mycelia is covered in tissue culture bottle after matrix, then uses dim light spontaneous fermentation culture 2 weeks of 400lx, this bacteria mode neither shadow
Ring the growth of Hericium erinaceus mycelia, moreover it is possible to energy consumption be greatly lowered, it may be more desirable to which later period low light culture can also obtain class
The Hericium erinaceus " mycelium " of entity；
S23, drying open tissue culture bottle and take out culture gained Hericium erinaceus mycoplasma after fermentation, mycoplasma is scattered and baking oven is used to carry out
Drying, drying temperature are 58 DEG C.
Further, step S11 further includes a major ingredient pre-treatment step: being poured into using the frying seed of jog's tears and wheat as major ingredient
The container of proper amount of clear water is filled, is stirred 3 minutes, is picked up showy impurity, filter out major ingredient, the major ingredient filtered out is put into cool-bag
In.
Further, the tissue culture bottle is the plant tissue culture bottle of 380ml, is packed into 129g hedgehog hydnum in each plant tissue culture bottle
Bacterium culture medium matter.
Further, the wheat original seed grain number accessed in each tissue culture bottle in step S21 is identical, to strive for that each bottle mycelia is raw
Length is as consistent as possible, facilitates management.
In above embodiments, major ingredient is cleaned and is pulled out after soaking, and filter pours into spice in 3 minutes or so after becoming water droplet to water flow
Container is poured into after mixing wheat bran and glucose, is weighed, conversion, is mixed thoroughly after adjusting water content to 45% or so.Using exempting to boil, exempt from
Drying not only can control water content of substrate, but also can reduce the boiling inconvenience of process and the big raw material of the amount of avoiding.
Preferably, Hericium erinaceus culture substrate further includes 94g frying Chinese yam, 94g frying Semen Lablab Album, 94g Fu in embodiment six
Siberian cocklebur, 94g lotus seeds.
Chinese yam is also known as Chinese yam, native potato, sweet potato Chinese yam, RHIIZOMA DIOSCOREAE from Henan of China, Chinese yam, white Chinese yam, is the herbal medicine that " China's book on Chinese herbal medicine " records, medicine
It is Dioscoreaceae plant Chinese yam dry rhizome with source.Chinese yam have nourish it is strong, it is aid digestion, hold back abnormal sweating, the effect of antidiarrheal, cure mainly
Insufficiency of the spleen diarrhoea, cough due to deficiency of the lung, indigestion.Frying Chinese yam strengthens the functions such as the invigorating spleen and reinforcing stomach of Chinese yam.
Semen Lablab Album is sweet in flavor, slightly warm in nature, there is an invigorating the spleen for eliminating dampness, inducing diuresis to remove edema, clear liver and improve vision and other effects.Pharmacopoeia Commission of the Ministry of Public Health
" Chinese Pharmacopoeia " compiled also is said: Semen Lablab Album " strengthening the spleen and stomach, clear summer-heat and damp ".Frying Semen Lablab Album enhances the invigorating the spleen to arrest diarrhea function of Semen Lablab Album
Effect.
Poria cocos is the dry sclerotia of polyporaceae fungus.For edema and little urine, phlegm retention anti-dazzle nervous, spleen eating less, loose stool
Diarrhea, confused and worried, insomnia of palpitating with fear.
Lotus seeds are the dry mature seed of nymphaeaceae plant lotus.With tonifying spleen and stopping diarrhea, the effect of mental-tranquilization.
The seed of jog's tears is the dry mature kernal of gramineae, there is clearing damp and promoting diuresis, invigorating the spleen to arrest diarrhea, eliminating impediment, apocenosis, solution
The effect of malicious dissipating bind.The frying seed of jog's tears helps the effect of seed of jog's tears plays invigorating the spleen to arrest diarrhea, reduces the irritation of the seed of jog's tears.
In six Hericium erinaceus culture substrate of embodiment be added frying Chinese yam, frying Semen Lablab Album, Poria cocos, lotus seeds, the seed of jog's tears and or
Wheat, wheat bran, glucose, the Hericium erinaceus mycoplasma that fermented and cultured comes out have the health-care efficacies such as certain strengthening the spleen and stomach, dampness removing antidiarrheal.
The above is only a preferred embodiment of the present invention, it should be understood that the present invention is not limited to described herein
Form should not be regarded as an exclusion of other examples, and can be used for other combinations, modifications, and environments, and can be at this
In the text contemplated scope, modifications can be made through the above teachings or related fields of technology or knowledge.And those skilled in the art institute into
Capable modifications and changes do not depart from the spirit and scope of the present invention, then all should be in the protection scope of appended claims of the present invention
It is interior.
Claims (10)
1. a kind of Hericium erinaceus culture substrate, which is characterized in that the ingredient including following parts by weight:
The seed of jog's tears and or 76~88 parts of wheat；
10~20 parts of wheat bran；
2~4 parts of glucose.
2. a kind of Hericium erinaceus culture substrate according to claim 1, it is characterised in that: the seed of jog's tears is the frying seed of jog's tears.
3. a kind of Hericium erinaceus culture substrate according to claim 1, it is characterised in that: the glucose is table grapes
Sugar.
4. a kind of Hericium erinaceus culture substrate according to claim 1, it is characterised in that: further include that frying Chinese yam, frying are white
Hyacinth bean, Poria cocos and lotus seeds.
5. a kind of side using the Hericium erinaceus culture substrate fermented and cultured Hericium erinaceus mycoplasma of any of claims 1-3
Method, which comprises the following steps:
S1, Hericium erinaceus culture substrate preparation the following steps are included:
S11, immersion, using the seed of jog's tears and or wheat as major ingredient, and by weight for major ingredient: water=1.0: 1.5~2.0 put major ingredient
Enter and impregnated in 100 DEG C of water, stops impregnating when weight reaches 170~180 kilograms after the water suction of every double centner major ingredient, so
Afterwards by major ingredient cleaning, pull out and filter to water flow at pouring into spice container after drop；
S12, spice, bottling pour into the spice container for filling major ingredient after mixing wheat bran and glucose, and Hericium erinaceus are cultivated
The water content of matrix controls between 42 ~ 46%, is then charged into tissue culture bottle, lid band film breather hole cover；
S13, sterilizing carry out high pressure with Hericium erinaceus culture substrate of the 0.15~0.16MPa to water content 42 ~ 46% in tissue culture bottle and go out
Bacterium；
S2, Hericium erinaceus mycoplasma fermented and cultured the following steps are included:
S21, inoculation, when temperature in tissue culture bottle is lower than 30 DEG C, aseptically first by Hericium erinaceus culture substrate in tissue culture bottle
Shaking is loose, then accesses the wheat original seed through shaking flask culture；
Tissue culture bottle after inoculation, is moved in culturing room by S22, fermented and cultured, and shading stands incubated at room temperature, room temperature 6~30 DEG C it
It is interior, after Hericium erinaceus mycelia covers with matrix in tissue culture bottle, then using dim light spontaneous fermentation culture 2~3 weeks of 50~400lx；
S23, drying open tissue culture bottle and take out Hericium erinaceus mycoplasma obtained by fermented and cultured, mycoplasma is scattered and is dried for standby.
6. the method for fermented and cultured Hericium erinaceus mycoplasma according to claim 5, which is characterized in that step S11 further includes one
A major ingredient pre-treatment step: using the seed of jog's tears and or wheat be put into as major ingredient and fill the container of proper amount of clear water, stir 3~5 minutes,
Showy impurity is picked up, major ingredient is filtered out, the major ingredient filtered out is put into cool-bag.
7. the method for fermented and cultured Hericium erinaceus mycoplasma according to claim 5, it is characterised in that: the tissue culture bottle is
The plant tissue culture bottle of 380ml, each plant tissue culture bottle is interior to be packed into 130 ± 1g Hericium erinaceus culture substrate.
8. the method for fermented and cultured Hericium erinaceus mycoplasma according to claim 5, it is characterised in that: when sterilizing in step S13
A length of 2h.
9. the method for fermented and cultured Hericium erinaceus mycoplasma according to claim 5, it is characterised in that: using baking in step S23
Case is dried, and drying temperature is 50~60 DEG C.
10. the method for fermented and cultured Hericium erinaceus mycoplasma according to claim 5, it is characterised in that: each group in step S21
The wheat original seed grain number accessed in training bottle is identical.
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| CN202210214395.XA CN114532151A (en) | 2018-11-30 | 2018-11-30 | Method for fermenting and culturing hericium erinaceus mycoplasm and application of hericium erinaceus mycoplasm |
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