CN109680017A - A kind of solid medium for being conducive to improve flavones content in phellinus igniarius mycelium - Google Patents
A kind of solid medium for being conducive to improve flavones content in phellinus igniarius mycelium Download PDFInfo
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- CN109680017A CN109680017A CN201910055052.1A CN201910055052A CN109680017A CN 109680017 A CN109680017 A CN 109680017A CN 201910055052 A CN201910055052 A CN 201910055052A CN 109680017 A CN109680017 A CN 109680017A
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Abstract
The invention discloses a kind of solid mediums for being conducive to improve flavones content in phellinus igniarius mycelium; its raw material includes: 20-30 parts of glucose, 20-30 parts of potato, 10-20 parts of soybean powder, 10-20 parts of casein, 5-10 parts of peptone, 5-8 parts of magnesium sulfate, 1-5 parts of calcium chloride, 1-5 parts of sodium chloride, 4-9 parts of saccharomycete, 20-26 parts of weed tree sawdust and 5-10 parts of fresh pig dung by weight; the casein is using fresh milk degreasing, and the present invention relates to edible mushroom culture technique fields.This is conducive to the solid medium for improving flavones content in phellinus igniarius mycelium; achieve the purpose that carry out fermentation using animal waste and weed tree sawdust and prepare culture substrate; realization directlys adopt the culture substrate that glucose, soybean powder, dry ferment and magnesium sulfate make high nutrition composition; substantially increase Phellinus bacterium culture medium matter nutrient content; the growth demand of Phellinus bacterium is met well; flavones content is lower in the Phellinus bacterium that culture substrate can be made to turn out, to be advantageous to the culture of Phellinus bacterium.
Description
Technical field
The present invention relates to edible mushroom culture technique field, specially one kind is conducive to improve flavones content in phellinus igniarius mycelium
Solid medium.
Background technique
Phellinus category Basidiomycotina, Hymenomycetes, Aphyllophorales, Hymenochaetaceae, Phellinus be phelliuns igniarius and
P .linteus, Phellinus are the perennial precious medicinal fungis of one kind developed in recent years, there is the laudatory title of " forest gold ",
The gynecological diseases such as metrorrhagia, blood strangury, leukorrhagia, amenorrhoea can be controlled, modern medicine study shows that Phellinus has antitumor, antibacterial, anti-fiber
Change, is anti-oxidant and the remarkable results such as improve the immunity of the human body, being the rare of biological antitumous effect first internationally recognized at present
Medicinal fungi, is widely used in the industries such as medicine, food, daily-use chemical industry, health care product, and the disease that the world today is most difficult to capture is cancer
Disease, due to the side effect of chemotherapeutics, the research of natural anti-cancer drugs is the hot spot of drug worker concern always, a large amount of popular
Disease learns investigation, zoopery and experiment in vitro result of study and shows that flavonoids has extensive suppression cancer and protective effect on cancer risk, especially
Think that cancer related with human body estrogen secretion, such as breast cancer, oophoroma, prostate cancer have preferable effect to some,
What is played a major role in Phellinus is flavonoids, and flavonoids is one of main active in nature medicinal plant, by its structure
It is divided into flavones, flavonols, flavanones, dioxy flavonols, isoflavones etc., current identified bioflavonoid has more than 6400 kinds,
There is flavonoids physiological activity, the Phellinus bacterium such as adjusting blood lipid, elimination oxygen radical, anti-oxidant, antitumor, antiviral to cultivate
It needs to be cultivated using unique culture medium in journey.
Current Phellinus bacterium culture medium matter nutrient content is lower, is not able to satisfy the growth demand of Phellinus bacterium, and existing culture
Flavones content is lower in the Phellinus bacterium that matrix is turned out, and can not achieve and is improved to Phellinus culture substrate to realize to Phellinus bacterium
It is cultivated well, is unable to reach and the purpose that fermentation prepares culture substrate, Bu Nengshi are carried out using animal waste and weed tree sawdust
The culture substrate that glucose, soybean powder, dry ferment and magnesium sulfate make high nutrition composition is now directlyed adopt, to give Phellinus bacterium
Culture bring greatly it is unfavorable.
Summary of the invention
(1) the technical issues of solving
In view of the deficiencies of the prior art, be conducive to improve flavones content in phellinus igniarius mycelium the present invention provides a kind of and consolidate
Body culture medium, it is lower to solve existing Phellinus bacterium culture medium matter nutrient content, is not able to satisfy the growth demand of Phellinus bacterium, and existing
Flavones content is lower in the Phellinus bacterium for having culture substrate to turn out, and can not achieve and is improved to Phellinus culture substrate to realize pair
Phellinus bacterium is cultivated well, is unable to reach and carries out the purpose that fermentation prepares culture substrate using animal waste and weed tree sawdust,
It can not achieve and directly adopt the problem of glucose, soybean powder, dry ferment and magnesium sulfate make high nutrition composition culture substrate.
(2) technical solution
In order to achieve the above object, the present invention is achieved by the following technical programs: one kind is conducive to improve phellinus liteus
The solid medium of flavones content in body, raw material include: 20-30 parts of glucose, 20-30 parts of potato, Huang by weight
10-20 parts of bean powder, 10-20 parts of casein, 5-10 parts of peptone, 5-8 parts of magnesium sulfate, 1-5 parts of calcium chloride, 1-5 parts of sodium chloride, ferment
Female bacterium 4-9 parts, 20-26 parts of weed tree sawdust and 5-10 parts of fresh pig dung.
Preferably, raw material includes following component: 25 parts of glucose, 25 parts of potato, 15 parts of soybean powder, casein 15
Part, 7 parts of peptone, 6 parts of magnesium sulfate, 3 parts of calcium chloride, 3 parts of sodium chloride, 6 parts of saccharomycete, 23 parts of weed tree sawdust and 7 parts of fresh pig dung.
Preferably, raw material includes following component: 20 parts of glucose, 20 parts of potato, 10 parts of soybean powder, casein 10
Part, 5 parts of peptone, 5 parts of magnesium sulfate, 1 part of calcium chloride, 1 part of sodium chloride, 4 parts of saccharomycete, 20 parts of weed tree sawdust and 5 parts of fresh pig dung.
Preferably, raw material includes following component: 30 parts of glucose, 30 parts of potato, 20 parts of soybean powder, casein 20
Part, 10 parts of peptone, 8 parts of magnesium sulfate, 5 parts of calcium chloride, 5 parts of sodium chloride, 9 parts of saccharomycete, 26 parts of weed tree sawdust and 10 parts of fresh pig dung.
Preferably, the casein is to be added lactic acid using fresh milk degreasing, pH is adjusted to 4.6, made casein protomere
It loses charge and solidifies precipitating.
Preferably, the glucose be select take off dry corn by smashing and grinding equipment obtain corn flour, then to corn
Water is added in powder and amylase stirs 2-3h, filters filter residue, can be obtained required glucose.
Preferably, the solid medium preparation method specifically includes the following steps:
S1, the weed tree sawdust of selection and fresh pig dung successively poured into pulverizer first, is sufficiently crushed, then will crushed
Material afterwards pours into mixing and blending machine, stirs 1-2h with the revolving speed of 400-600r/min, fills weed tree sawdust with fresh pig dung
Divide mixing, obtain culture material, the saccharomycete of selection is inoculated in culture material later, is stirred with the revolving speed of 500-600r/min
Mixing 20-30min is mixed, 20-25d is sealed by fermentation, can be obtained Nutrious fermented matrix；
S2, the potato of selection is first subjected to peeling processing, then potato is put into boiler plus water submerged, heating are boiled
Boiling, persistently boils 10-20min, later smashs cooked potato in boiler to pieces, and 20-25min is boiled in heating again, then
Air-cooled 30-40min is stood, potato nutritional suspension can be obtained；
S3, the nutrition suspension that S2 is obtained is poured into the Nutrious fermented Medium Culture in S1, is stirred with revolving speed for 400-600r/min
30-40min is mixed, then by the glucose of selection, soybean powder, casein, peptone, magnesium sulfate, calcium chloride and sodium chloride are successively
It is added in mixing and blending machine, 1-2h is stirred with the revolving speed of 700-900r/min, can be obtained culture medium；
S4, the culture medium raw material for obtaining S3 thermal dehydration to the moisture content at 37-48 DEG C are the 50- of total weight
66%, it is then wrapped up using aseptic thin-film, 10-25min is handled using 88-100 DEG C of vapor high pressure sterilization later, can be obtained
Solid medium.
(3) beneficial effect
The present invention provides a kind of solid mediums for being conducive to improve flavones content in phellinus igniarius mycelium.With the prior art
Compared to have it is following the utility model has the advantages that this be conducive to improve phellinus igniarius mycelium in flavones content solid medium, by its original
Material includes: 20-30 parts of glucose, 20-30 parts of potato, 10-20 parts of soybean powder, 10-20 parts of casein, albumen by weight
5-10 parts of peptone, 5-8 parts of magnesium sulfate, 1-5 parts of calcium chloride, 1-5 parts of sodium chloride, 4-9 parts of saccharomycete, 20-26 parts of weed tree sawdust and fresh pig
5-10 parts of excrement, casein is added lactic acid, pH is adjusted to 4.6 using fresh milk degreasing, make casein protomere lose charge and
Solidification precipitating, glucose be select take off dry corn by smashing and grinding equipment obtain corn flour, water is then added into corn flour
2-3h is stirred with amylase, filter residue is filtered, can be obtained required glucose, the preparation method of solid medium specifically includes following
Step: S1, successively pouring into the weed tree sawdust of selection and fresh pig dung in pulverizer first, sufficiently crushed, after then crushing
Material pour into mixing and blending machine, 1-2h is stirred with the revolving speed of 400-600r/min, carries out weed tree sawdust and fresh pig dung abundant
Mixing obtains culture material, the potato of selection first S2, is carried out peeling processing, then potato is put into boiler and adds water
It floods, heating is boiled, and 10-20min is persistently boiled, and later smashs cooked potato in boiler to pieces, and 20- is boiled in heating again
25min is then allowed to stand air-cooled 30-40min, and potato nutritional suspension can be obtained, the nutrition suspension that S2 is obtained S3, is poured into S1
In Nutrious fermented Medium Culture, with revolving speed be 400-600r/min stir 30-40min, then by the glucose of selection, soya bean
Powder, casein, peptone, magnesium sulfate, calcium chloride and sodium chloride are added sequentially in mixing and blending machine, with 700-900r/min's
Revolving speed stirs 1-2h, can be obtained culture medium, S4, the culture medium raw material for obtaining S3 at 37-48 DEG C thermal dehydration to moisture
Content is the 50-66% of total weight, is then wrapped up using aseptic thin-film, later using 88-100 DEG C of vapor high pressure sterilization processing
Solid medium can be obtained, it can be achieved that being improved to Phellinus culture substrate to realize and carry out very to Phellinus bacterium in 10-25min
Good culture achievees the purpose that fermentation is carried out using animal waste and weed tree sawdust prepares culture substrate, and realization directlys adopt grape
Sugar, soybean powder, dry ferment and magnesium sulfate make the culture substrate of high nutrition composition, substantially increase Phellinus bacterium culture medium matter battalion
Content to be supported, meets the growth demand of Phellinus bacterium well, flavones content is lower in the Phellinus bacterium that culture substrate can be made to turn out,
To be advantageous to the culture of Phellinus bacterium.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
Technical staff's every other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.
The embodiment of the present invention provides three kinds of technical solutions: a kind of solid for being conducive to improve flavones content in phellinus igniarius mycelium
The preparation method of culture medium, specifically includes following embodiment:
Embodiment 1
S1,23 parts of weed tree sawdusts of selection and 7 parts of fresh pig dungs are successively poured into pulverizer first, is sufficiently crushed, so
Smashed material is poured into mixing and blending machine afterwards, 1.5h is stirred with the revolving speed of 500r/min, make weed tree sawdust and fresh pig dung into
Row is sufficiently mixed, and obtains culture material, and 6 parts of saccharomycete of selection are inoculated in culture material later, with turning for 550r/min
Speed is stirred 25min, is sealed by fermentation 23d, can be obtained Nutrious fermented matrix；
S2,25 portions of potatos of selection are first subjected to peeling processing, then potato is put into boiler and adds water submerged, adds
Heat is boiled, and 15min is persistently boiled, and later smashs cooked potato in boiler to pieces, and 23min is boiled in heating again, then quiet
Cold 35min is emptied, potato nutritional suspension can be obtained；
S3, the nutrition suspension that S2 is obtained is poured into the Nutrious fermented Medium Culture in S1, is 500r/min stirring with revolving speed
35min, then by 25 parts of glucose of selection, 15 parts of soybean powders, 15 parts of casein, 7 parts of peptones, 6 parts of magnesium sulfate, 3 parts of chlorine
Change calcium and 3 parts of sodium chloride are added sequentially in mixing and blending machine, 1.5h is stirred with the revolving speed of 800r/min, can be obtained culture
Base；
S4, the culture medium raw material for obtaining S3 thermal dehydration to the moisture content at 43 DEG C are the 60% of total weight, then
It is wrapped up using aseptic thin-film, handles 18min using 93 DEG C of vapor high pressure sterilizations later, solid medium can be obtained.
Embodiment 2
S1,20 parts of weed tree sawdusts of selection and 5 parts of fresh pig dungs are successively poured into pulverizer first, is sufficiently crushed, so
Smashed material is poured into mixing and blending machine afterwards, 1h is stirred with the revolving speed of 400r/min, carries out weed tree sawdust and fresh pig dung
It is sufficiently mixed, obtains culture material, the saccharomycete of selection is inoculated in culture material later, is stirred with the revolving speed of 500r/min
20min is mixed, 20d is sealed by fermentation, can be obtained Nutrious fermented matrix；
S2,20 portions of potatos of selection are first subjected to peeling processing, then potato is put into boiler and adds water submerged, adds
Heat is boiled, and 10min is persistently boiled, and later smashs cooked potato in boiler to pieces, and 20min is boiled in heating again, then quiet
Cold 30min is emptied, potato nutritional suspension can be obtained；
S3, the nutrition suspension that S2 is obtained is poured into the Nutrious fermented Medium Culture in S1, is 400r/min stirring with revolving speed
30min, then by 20 parts of glucose of selection, 10 parts of soybean powders, 10 parts of casein, 5 parts of peptones, 5 parts of magnesium sulfate, 1 part of chlorine
Change calcium and 1 part of sodium chloride is added sequentially in mixing and blending machine, 1h is stirred with the revolving speed of 700r/min, can be obtained culture medium；
S4, the culture medium raw material for obtaining S3 thermal dehydration to the moisture content at 37 DEG C are the 50% of total weight, then
It is wrapped up using aseptic thin-film, handles 10min using 88 DEG C of vapor high pressure sterilizations later, solid medium can be obtained.
Embodiment 3
S1,26 parts of weed tree sawdusts of selection and 10 parts of fresh pig dungs are successively poured into pulverizer first, is sufficiently crushed, so
Smashed material is poured into mixing and blending machine afterwards, 2h is stirred with the revolving speed of 600r/min, carries out weed tree sawdust and fresh pig dung
It is sufficiently mixed, obtains culture material, the saccharomycete of selection is inoculated in culture material later, is stirred with the revolving speed of 600r/min
30min is mixed, 25d is sealed by fermentation, can be obtained Nutrious fermented matrix；
S2,30 portions of potatos of selection are first subjected to peeling processing, then potato is put into boiler and adds water submerged, adds
Heat is boiled, and 20min is persistently boiled, and later smashs cooked potato in boiler to pieces, and 25min is boiled in heating again, then quiet
Cold 40min is emptied, potato nutritional suspension can be obtained；
S3, the nutrition suspension that S2 is obtained is poured into the Nutrious fermented Medium Culture in S1, is 600r/min stirring with revolving speed
40min, then by 30 parts of glucose of selection, 20 parts of soybean powders, 20 parts of casein, 10 parts of peptones, 8 parts of magnesium sulfate, 5 parts of chlorine
Change calcium and 5 parts of sodium chloride are added sequentially in mixing and blending machine, 2h is stirred with the revolving speed of 900r/min, can be obtained culture medium；
S4, the culture medium raw material for obtaining S3 thermal dehydration to the moisture content at 48 DEG C are the 66% of total weight, then
It is wrapped up using aseptic thin-film, handles 25min using 100 DEG C of vapor high pressure sterilizations later, solid medium can be obtained.
Effect example
Certain cultivating edible family can be marked respectively using the solid culture matrix of 1-3 of embodiment of the present invention preparation Phellinus bacterium
For No.1 culture substrate, No. two culture substrates and No. three culture substrates, then respectively to No.1 culture substrate, No. two culture substrates
Phellinus bacterium are inoculated with No. three culture substrates, culture is then carried out and respectively obtains No.1 Phellinus strain, No. two Phellinus strains and No. three
Phellinus strain carries out flavones detection, detection knot to No.1 Phellinus strain, No. two Phellinus strains and No. three Phellinus strains respectively later
Fruit shows that the content of flavones in No.1 Phellinus strain is higher than the content of flavones in No. two Phellinus strains and No. three Phellinus strains, therefore
Phellinus bacterium culture medium matter prepared by embodiment 1 is best.
It should be noted that, in this document, relational terms such as first and second and the like are used merely to a reality
Body or operation are distinguished with another entity or operation, are deposited without necessarily requiring or implying between these entities or operation
In any actual relationship or order or sequence.Moreover, the terms "include", "comprise" or its any other variant are intended to
Non-exclusive inclusion, so that the process, method, article or equipment including a series of elements is not only wanted including those
Element, but also including other elements that are not explicitly listed, or further include for this process, method, article or equipment
Intrinsic element.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (7)
1. a kind of solid medium for being conducive to improve flavones content in phellinus igniarius mycelium, it is characterised in that: its raw material is by weight
Part ratio includes: 20-30 parts of glucose, 20-30 parts of potato, 10-20 parts of soybean powder, 10-20 parts of casein, peptone 5-10
Part, 5-8 parts of magnesium sulfate, 1-5 parts of calcium chloride, 1-5 parts of sodium chloride, 4-9 parts of saccharomycete, 20-26 parts of weed tree sawdust and fresh pig dung 5-10
Part.
2. a kind of solid medium for being conducive to improve flavones content in phellinus igniarius mycelium according to claim 1, special
Sign is: its raw material includes following component: 25 parts of glucose, 25 parts of potato, 15 parts of soybean powder, 15 parts of casein, peptone 7
Part, 6 parts of magnesium sulfate, 3 parts of calcium chloride, 3 parts of sodium chloride, 6 parts of saccharomycete, 23 parts of weed tree sawdust and 7 parts of fresh pig dung.
3. a kind of solid medium for being conducive to improve flavones content in phellinus igniarius mycelium according to claim 1, special
Sign is: its raw material includes following component: 20 parts of glucose, 20 parts of potato, 10 parts of soybean powder, 10 parts of casein, peptone 5
Part, 5 parts of magnesium sulfate, 1 part of calcium chloride, 1 part of sodium chloride, 4 parts of saccharomycete, 20 parts of weed tree sawdust and 5 parts of fresh pig dung.
4. a kind of solid medium for being conducive to improve flavones content in phellinus igniarius mycelium according to claim 1, special
Sign is: its raw material includes following component: 30 parts of glucose, 30 parts of potato, 20 parts of soybean powder, 20 parts of casein, peptone
10 parts, 8 parts of magnesium sulfate, 5 parts of calcium chloride, 5 parts of sodium chloride, 9 parts of saccharomycete, 26 parts of weed tree sawdust and 10 parts of fresh pig dung.
5. a kind of solid training for being conducive to improve flavones content in phellinus igniarius mycelium according to any one of claims 1-4
Support base, it is characterised in that: the casein is to be added lactic acid using fresh milk degreasing, pH is adjusted to 4.6, made the micro- glue of casein
Grain loses charge and solidifies precipitating.
6. a kind of solid training for being conducive to improve flavones content in phellinus igniarius mycelium according to any one of claims 1-4
Support base, it is characterised in that: the glucose be select take off dry corn by smashing and grinding equipment obtain corn flour, then to corn
Water is added in powder and amylase stirs 2-3h, filters filter residue, can be obtained required glucose.
7. a kind of solid training for being conducive to improve flavones content in phellinus igniarius mycelium described in -6 any one according to claim 1
Support base, it is characterised in that: the preparation method of the solid medium specifically includes the following steps:
S1, the weed tree sawdust of selection and fresh pig dung successively poured into pulverizer first, is sufficiently crushed, it then will be smashed
Material pours into mixing and blending machine, stirs 1-2h with the revolving speed of 400-600r/min, carries out weed tree sawdust and fresh pig dung sufficiently mixed
It closes, obtains culture material, the saccharomycete of selection is inoculated in culture material later, it is mixed with the revolving speed stirring of 500-600r/min
20-30min is closed, 20-25d is sealed by fermentation, can be obtained Nutrious fermented matrix；
S2, the potato of selection is first subjected to peeling processing, then potato is put into boiler plus water submerged, heating are boiled,
10-20min is persistently boiled, later smashs cooked potato in boiler to pieces, 20-25min is boiled in heating again, is then allowed to stand
Potato nutritional suspension can be obtained in air-cooled 30-40min；
S3, the nutrition suspension that S2 is obtained is poured into the Nutrious fermented Medium Culture in S1, is that 400-600r/min stirs 30- with revolving speed
40min, then by the glucose of selection, soybean powder, casein, peptone, magnesium sulfate, calcium chloride and sodium chloride are added sequentially to
In mixing and blending machine, 1-2h is stirred with the revolving speed of 700-900r/min, can be obtained culture medium；
S4, the culture medium raw material for obtaining S3 thermal dehydration to the moisture content at 37-48 DEG C are the 50-66% of total weight, so
It is wrapped up afterwards using aseptic thin-film, 10-25min is handled using 88-100 DEG C of vapor high pressure sterilization later, solid training can be obtained
Support base.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910055052.1A CN109680017A (en) | 2019-01-21 | 2019-01-21 | A kind of solid medium for being conducive to improve flavones content in phellinus igniarius mycelium |
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| CN201910055052.1A CN109680017A (en) | 2019-01-21 | 2019-01-21 | A kind of solid medium for being conducive to improve flavones content in phellinus igniarius mycelium |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112195104A (en) * | 2020-07-17 | 2021-01-08 | 江苏科技大学 | Culture medium for identifying phellinus igniarius of mulberry tree and preparation method and application thereof |
| CN114369539A (en) * | 2022-01-11 | 2022-04-19 | 吉林大学 | Preparation method of culture medium taking agricultural wastes as raw materials, culture medium and application |
| TWI766394B (en) * | 2020-10-19 | 2022-06-01 | 葡萄王生技股份有限公司 | Use of phellinus linteus for preparing composition of improving sarcopenia |
-
2019
- 2019-01-21 CN CN201910055052.1A patent/CN109680017A/en not_active Withdrawn
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112195104A (en) * | 2020-07-17 | 2021-01-08 | 江苏科技大学 | Culture medium for identifying phellinus igniarius of mulberry tree and preparation method and application thereof |
| CN112195104B (en) * | 2020-07-17 | 2021-08-31 | 江苏科技大学 | Culture medium for identifying phellinus igniarius of mulberry tree and preparation method and application thereof |
| TWI766394B (en) * | 2020-10-19 | 2022-06-01 | 葡萄王生技股份有限公司 | Use of phellinus linteus for preparing composition of improving sarcopenia |
| US11944659B2 (en) | 2020-10-19 | 2024-04-02 | Grape King Bio Ltd | Method for improving sarcopenia by using Phellinus linteus |
| CN114369539A (en) * | 2022-01-11 | 2022-04-19 | 吉林大学 | Preparation method of culture medium taking agricultural wastes as raw materials, culture medium and application |
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