CN109279700A - Administer leavening, composite bacteria agent, the Preparation method and use of blue algae polluted water body - Google Patents
Administer leavening, composite bacteria agent, the Preparation method and use of blue algae polluted water body Download PDFInfo
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- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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Abstract
The application provides a kind of for administering the leavening of blue algae polluted water body, including the molten algae dominant strain freeze-dried powder screened from blue algae polluted water body and the calcium lime powder as medium component, brown sugar, sodium bicarbonate, edible salt, yeast powder, rice straw powder, plant ash and vanadic anhydride, it also provides a kind of for administering the composite bacteria agent of blue algae polluted water body, is protected from light and is fermented through anaerobism with rice straw powder, reed powder and rice husk powder by the leavening.The application also provides the preparation method of the leavening and composite bacteria agent, the purposes for administering blue algae polluted water body, and the method for administering blue algae polluted water body.Molten algae dominant strain in the composite bacteria agent can promote Cells of Blue-green Algae apoptosis, and a large amount of lactic acid bacterias in the composite bacteria agent can absorb the substances such as carbohydrate, the cellulose of dead Cells of Blue-green Algae release.The composite bacteria agent can direct plunge into blue algae polluted water body, and easy to use efficient, the rice straw powder, reed powder and rice husk powder raw material sources for preparing composite bacteria agent are wide, at low cost.
Description
Technical field
The present invention relates to water pollutions to administer field, and in particular to a kind of leavening for administering blue algae polluted water body and
Preparation method, a kind of composite bacteria agent for administering blue algae polluted water body and preparation method thereof, the leavening and the compound bacteria
Agent is used to administer the purposes of blue algae polluted water body, and the method for administering blue algae polluted water body.
Background technique
With the growth of human population and the development of industrial or agricultural, not plus administers or administer incomplete sanitary sewage, industry
Waste water, organic waste and domestic animals and fowls excrement and agriculture largely enter in surface water body to fertilize, cause ammonia nitrogen in water body, total
The nutriments such as phosphorus are excessive, eventually lead to water eutrophication.Water eutrophication causes some planktonic algaes in water body fast
Speed breeding, the decline of Dissolved Oxygen in Water amount, water quality deterioration, fish and other biological mortality.Mistake of the planktonic algae in water body
Amount breeding will form wawter bloom, be mostly cyanobacterial bloom in poisons in freshwater, then be red tide or red tide in ocean.
Currently, the pollution of the poisons in freshwater such as many rivers and lakes ponds in China causes cyanobacterial bloom phenomenon extremely serious, lake
The mortalities such as middle fishes and shrimps greatly destroy the water ecology and ambient enviroment in rivers and lakes pond, have severely impacted work
Agricultural production results in significant economic losses.There is also algae toxins in polluted-water, will affect tap water source quality, right
The life and health of the people constitutes potential threat.
The method for removing cyanobacterial bloom in the prior art has mechanical Refloatation method, chemicals to administer method and microbial treatment
Method.Mechanical Refloatation method salvages cyanobacteria by mechanical equipment, needs a large amount of manpower and material resources, can not eradicate water body indigo plant
Algae belongs to emergency, subsequent property governing measure.Chemicals administers method and kills cyanobacteria by applying chemicals into water body,
But other biological in lake can usually be damaged simultaneously, cause the secondary pollution of water body.Microbial treatment method is by water
Apply microbial bacterial agent in body, absorption, degradation, conversion fall the Eutrophication materials such as nitrogen phosphorus in polluted-water, reduce the battalion of cyanobacteria
Substance supply is supported, and secretes the bioactive substance for inhibiting cyanobacteria, kills cyanobacteria present in water body.
From the viewpoint of ecology, microbial treatment method is a kind of dirt of the reduction eutrophication water of ecological, environmental protective
Dye object and the method for inhibiting cyanobacteria.It finds more suitable, more effective cyanobacteria and administers microbial bacterial agent, be always those skilled in the art
The target that member constantly pursues.
Summary of the invention
The leavening and composite bacteria agent that the object of the present invention is to provide new for administering blue algae polluted water body and they
Preparation method and use, a kind of new method of improvement blue algae polluted water body is especially provided.
To achieve the above objectives, the present invention provides technical solutions below.
In a first aspect, the present invention provide it is a kind of for administering the leavening of blue algae polluted water body, the leavening include with
The following component of lower parts by weight: molten 1 part of algae dominant strain freeze-dried powder, the calcium lime powder 10-30 screened from blue algae polluted water body
Part, 20-40 parts of brown sugar, 20-40 parts of sodium bicarbonate, 40-60 parts of edible salt, 40-60 parts of yeast powder, 80-120 parts of rice straw powder, vegetation
It is 20-40 parts grey, 0.1-0.5 parts of vanadic anhydride.
Preferably, which includes the following component of following parts by weight: the molten algae advantage screened in blue algae polluted water body
1 part of bacterial strain freeze-dried powder, 15-25 parts of calcium lime powder, 25-35 parts of brown sugar, 25-35 parts of sodium bicarbonate, 45-55 parts of edible salt, yeast powder
45-55 parts, 90-110 parts of rice straw powder, 25-35 parts of plant ash, 0.2-0.4 parts of vanadic anhydride.
It is highly preferred that the leavening includes the following component of following parts by weight: the molten algae screened in blue algae polluted water body is excellent
1 part of gesture bacterial strain freeze-dried powder, 20 parts of calcium lime powder, 30 parts of brown sugar, 30 parts of sodium bicarbonate, 50 parts of edible salt, 50 parts of yeast powder, rice straw powder
100 parts, 30 parts of plant ash, 0.3 part of vanadic anhydride.
In second aspect, the present invention provides the preparation side of the leavening for administering blue algae polluted water body of first aspect
Method, method includes the following steps:
Molten algae dominant strain is screened from blue algae polluted water body, and is prepared into bacterial strain freeze-dried powder；
Calcium lime powder, brown sugar, sodium bicarbonate, edible salt, yeast powder, rice straw powder, vegetation are weighed by specified weight ratio
Ash, vanadic anhydride are uniformly mixed and obtain mixture；
It takes 1 parts by weight of bacterial strain freeze-dried powder to mix with the mixture, obtains the leavening.
Preferably, the leavening sealing preservation is spare in 4 DEG C of refrigerators.
In the third aspect, the present invention provides a kind of for administering the composite bacteria agent of blue algae polluted water body, the composite bacteria agent packet
Include the following component of following parts by weight: 15-25 parts of 60-70 parts of 1 part of leavening, rice straw powder, reed powder, the rice husk powder of first aspect
10-20 parts, by the way that the ingredient is fermented under the conditions of anaerobism is protected from light.
Preferably, which includes the following component of following parts by weight: 1 part of the leavening of first aspect, rice straw powder
65 parts, 20 parts of reed powder, 15 parts of rice husk powder, by the way that the ingredient is fermented under the conditions of anaerobism is protected from light.
In fourth aspect, the present invention provides the preparation side of the composite bacteria agent for administering blue algae polluted water body of the third aspect
Method, method includes the following steps:
Rice straw powder, reed powder and rice husk powder are weighed by specified weight ratio, is uniformly mixed and obtains fermentation base-material；
The leavening for taking 1 parts by weight is dispersed in water to obtain the aqueous dispersions of the leavening；
The aqueous dispersions are poured into the fermentation base-material, are stirred evenly, ferments 15-20 days, obtains under the conditions of anaerobism is protected from light
To the composite bacteria agent.
At the 5th aspect, the present invention provides the leavening of first aspect and the composite bacteria agent of the third aspect for administering cyanobacteria
The purposes of polluted-water.
At the 6th aspect, the present invention provides a kind of method for administering blue algae polluted water body, and this method includes by the third aspect
Composite bacteria agent put into blue algae polluted water body to be administered.
Preferably, based on 1 meter of mean depth, blue algae polluted water body puts into 15-20 kilograms of the composite bacteria agent per acre.
Due to using the technology described above, the beneficial effects of the present invention are:
1. the molten algae dominant strain in leavening and composite bacteria agent of the invention can grow and be secreted in water body inhibition, kill
Go out the active material of cyanobacteria, promotes Cells of Blue-green Algae apoptosis.The a large amount of lactic acid bacterias generated after the composite bacteria agent is fermented can be in water body
The substances such as middle growth and the carbohydrate, the cellulose that adsorb, degrade, converting, being discharged using dead Cells of Blue-green Algae, reduce these objects
The eutrophication pollution of confrontation water body, and these substances can also be in favor of the chlorella growth beneficial to aquatile.Water body
The lactic acid bacteria and chlorella of middle raised growth can by water body protozoan and fishes and shrimps ingest.Therefore, through the invention
Application of the composite bacteria agent in water body, is suppressed the cyanobacteria of blue algae polluted water body, maintains beneficial microbe and water in water body
Biological and ecological balance, it is long-term to keep the limpid pollution-free of water body.
It is pointedly prepared for specific blue algae polluted water body to be processed for administering cyanobacteria 2. the present invention is able to achieve
The leavening and composite bacteria agent of polluted-water.The present invention from blue algae polluted water body to be processed by screening the molten algae advantage of original inhabitants
Bacterial strain, the leavening and composite bacteria agent of preparation cost invention can targetedly be used for the blue algae polluted water to be processed very much
Body, the general microbial bacterial agent specific aim overcome in the market be not strong, it is not easy to the problem of adapting to specific body of water.
3. in leavening and composite bacteria agent of the invention, there are vanadic anhydrides.The inventors discovered that vanadic anhydride sheet
Although body has toxicity, dosage is controlled in a small amount of range, has the function of stable and activating microorganisms bacterial strain instead.Cause
This, vanadic anhydride can stablize molten algae dominant strain freeze-dried powder in leavening of the invention, be protected from light hair in subsequent anaerobism
Ferment can activate molten algae dominant strain and lactic acid bacteria, have the function of key to leavening and composite bacteria agent of the invention.
4. blue algae polluted water body administering method of the invention is very simple and convenient efficient, can be by composite bacteria agent of the invention
It directly launches into blue algae polluted water body, increases significantly the quantity of the molten algae dominant strain in blue algae polluted water body, rapidly comprehensively
It kills cyanobacteria and administers other pollutions, the minimizing technology for solving cyanobacterial bloom in the prior art consumes a large amount of financial resources manpower objects
Power, be easy to cause secondary pollution, limited efficacy, cyanobacteria be easy demutation the problems such as.
5. it is lower that rice straw powder used in the preparation method of composite bacteria agent of the invention, reed powder, rice husk powder belong to value
Raw material, whole nation major part rural area has flood tide output every year, is easy to obtain, at low cost, is greatly saved of the invention compound
The production cost of microbial inoculum.
Specific embodiment
Below by specific embodiment, invention is further described in detail.It should be pointed out that herein, term
" comprising " refers generally to open meaning, but in actual implementation of the invention, can also refer to exclusive sense.
Microorganism fungus kind currently on the market is many kinds of, is the strain as obtained by culture medium progress artificial culture mostly
Be from the market or other biological laboratory and store microorganism scientific research institutions buy.The present inventor in a large amount of research and
It is found in practice, is stored in the microbial population of each laboratory and scientific research institutions, in the prevalence of serious phenomenon of degenerating.It deposits
Put that the time is longer, and spawn degeneration is more serious, using such Spawn incubation and the microorganism fungus kind prepared, in the prevalence of
Preparation cost is high, service efficiency is low, to the regulation effect difference of blue algae polluted water body or unstable, the feelings such as can not be widely applied
Condition cannot tackle the problem at its root.
It is different from various microorganism fungus kinds in the market, leavening for administering blue algae polluted water body of the invention and compound
Molten algae dominant strain used in microbial inoculum is that fresh screening obtains from natural blue algae polluted water body.It, will for the ease of saving
The molten algae dominant strain that screening obtains carries out freeze-drying and is prepared into freeze-dried powder.The technology of strain freeze-drying is art technology
Well known to personnel.
It is summarized as follows in the present invention from the method that blue algae polluted water body screens molten algae dominant strain: acquisition blue algae polluted water body
Sample；First with 0.8 μm of Fibrous membrane filtration, gained filtrate uses 0.22 μm of Fibrous membrane filtration again, collects 0.22 μm of tunica fibrosa,
It is rinsed with deionized water, prepares the bacterium solution containing potential molten algae dominant strain；Prepare molten algae dominant strain culture medium, inverted plate, institute
It obtains bacterium solution spread plate to be cultivated, the suitable plate of selecting bacteria stand density supplies subsequent picking single colonie；By containing for acquisition
The water sample of cyanobacteria is sub-packed in triangular flask, from plate picking single colonie, is inoculated in a triangular flask respectively, triangular flask is put into
It is cultivated on rotary shaker；It selects cyanobacteria and one or several triangular flasks of obvious yellow, molten algae occurs, then use Fibrous membrane filtration
It handles algae solution and obtains potential molten algae dominant strain, then carry out plate culture again and obtain single colonie, as required molten algae is excellent
Gesture bacterial strain.The method program of molten algae dominant strain is specifically screened referring to embodiment 1.
It should be pointed out that China has a vast territory, the cyanobacteria type and quantity of the blue algae polluted water body of various regions are had differences, because
And there is also differences for molten algae dominant strain present in cyanobacteria sewage water body.Of the invention has the prominent advantages that, by to from
The molten algae dominant strain of original inhabitants, the hair for being used to administer blue algae polluted water body of preparation cost invention are screened in the blue algae polluted water body of reason
Ferment agent and composite bacteria agent can targetedly be used for the blue algae polluted water body to be processed, overcome in the market general very much
Microbial bacterial agent specific aim is not strong, it is not easy to the problem of adapting to specific body of water.
The first aspect of the present invention provides a kind of for administering the leavening of blue algae polluted water body, which includes from indigo plant
The molten algae dominant strain freeze-dried powder screened in algae pollution water body and the calcium lime powder as starter medium ingredient, brown sugar,
Sodium bicarbonate, edible salt, yeast powder, rice straw powder, plant ash and vanadic anhydride.The molten algae advantage screened from blue algae polluted water body
Bacterial strain can inhibit, kill the algae such as cyanobacteria in blue algae polluted water body.Molten algae dominant strain is lyophilized in starter medium ingredient
Powder plays stable effect, in a kind of compound bacteria prepared for administering blue algae polluted water body that the fourth aspect of the present invention provides
Play the role of the molten algae dominant strain of activation and lactic acid bacteria in the method for agent, this by below to the fourth aspect of the present invention into
It is described in detail in the description of one step.
The content of medium component in leavening for administering blue algae polluted water body of the invention can be in a wide range
It changes.Therefore, in some embodiments of the present invention, by the molten algae advantage screened in the slave blue algae polluted water body of 1 parts by weight
Bacterial strain freeze-dried powder meter, the leavening include the following medium component of following parts by weight: 10-30 parts of calcium lime powder, brown sugar 20-40
Part, 20-40 parts of sodium bicarbonate, 40-60 parts of edible salt, 40-60 parts of yeast powder, 80-120 parts of rice straw powder, 20-40 parts of plant ash, five
0.1-0.5 parts of V 2 O.
It is excellent by the molten algae screened in the slave blue algae polluted water body of 1 parts by weight in certain preferred embodiments of the invention
Gesture bacterial strain freeze-dried powder meter, the leavening include the following medium component of following parts by weight: 15-25 parts of calcium lime powder, brown sugar 25-
35 parts, 25-35 parts of sodium bicarbonate, 45-55 parts of edible salt, 45-55 parts of yeast powder, 90-110 parts of rice straw powder, 25-35 parts of plant ash,
0.2-0.4 parts of vanadic anhydride.
It is molten by being screened in the slave blue algae polluted water body of 1 parts by weight in a further preferred embodiment of the invention
Algae dominant strain freeze-dried powder meter, the leavening include the following medium component of following parts by weight: 20 parts of calcium lime powder, brown sugar 30
Part, 30 parts of sodium bicarbonate, 50 parts of edible salt, 50 parts of yeast powder, 100 parts of rice straw powder, 30 parts of plant ash, 0.3 part of vanadic anhydride.
Correspondingly, the second aspect of the present invention provides the system of the leavening for administering blue algae polluted water body of first aspect
Preparation Method method includes the following steps: screening molten algae dominant strain from blue algae polluted water body, and is prepared into bacterial strain freeze-drying
Powder；Medium component calcium lime powder, brown sugar, sodium bicarbonate, edible salt, yeast are weighed by the weight ratio that first aspect is specified
Powder, rice straw powder, plant ash, vanadic anhydride are uniformly mixed and obtain mixture；1 parts by weight of bacterial strain freeze-dried powder are taken to mix with this
Object mixing, obtains the leavening.
The method of molten algae dominant strain is screened from blue algae polluted water body as described above, and the method for preparing bacterial strain freeze-dried powder
It is well known to those skilled in the art.It can be in department stores as the brown sugar of medium component, sodium bicarbonate, edible salt, yeast powder
In commercially available acquisition, calcium lime powder and vanadic anhydride can chemically reagent suppliers obtain, and dry rice straw can be used through powder in rice straw powder
Broken machine is ground into 1mm or the powder of partial size below obtains, and plant ash can be used dry faggot burning and ashes is taken to obtain.Each culture
Based component is ground in addition to saccharomycete using mortar, so that each ingredient crushes and be mixed to get homogeneous mixture, is then added again
Enter yeast gentle agitation uniformly to obtain a homogeneous mixture again, saccharomycete can be avoided damage in this way.Bacterial strain freeze-dried powder and gained are mixed
The mixed mode for closing object is to weigh the bacterial strain freeze-dried powders of 1 parts by weight to be added in the mixture, is softly mixed thoroughly, paid attention to spoon
It not exert oneself very much, in order to avoid damage strain cell.The leavening being prepared preferably sealing preservation is spare in 4 DEG C of refrigerators.
The third aspect of the present invention provides a kind of for administering the composite bacteria agent of blue algae polluted water body, which includes
Leavening and rice straw powder, the reed powder and rice husk powder of first aspect are fermented by the way that these ingredients progress anaerobism to be protected from light.
Dry rice straw, dry reed and gulch shell can be respectively adopted through pulverizer in used rice straw powder, reed powder and rice husk powder
The powder for being ground into 1mm or partial size below obtains.The content of rice straw powder, reed powder and rice husk powder can change in a wide range.
Therefore, in some embodiments of the present invention, the composite bacteria agent for administering blue algae polluted water body includes the first of 1 parts by weight
The leavening of aspect and rice straw powder, the reed powder of 15-25 parts by weight and the rice husk powder of 10-20 parts by weight of 60-70 parts by weight lead to
It crosses for these ingredients progress anaerobism to be protected from light and ferment.In a preferred embodiment in accordance with this invention, for administering cyanobacteria
The composite bacteria agent of polluted-water includes the leavening of the first aspect of 1 parts by weight and rice straw powder, 20 parts by weight of 65 parts by weight
The rice husk powder of reed powder and 15 parts by weight is fermented by the way that these ingredients progress anaerobism to be protected from light.
Correspondingly, the fourth aspect of the present invention provides the compound bacteria for being used to administer blue algae polluted water body of the preparation third aspect
The method of agent, method includes the following steps: weighing rice straw powder, reed powder and husk by the weight ratio that the third aspect is specified
Powder is uniformly mixed and obtains fermentation base-material；The leavening for taking 1 parts by weight is dispersed in water to obtain the aqueous dispersions of the leavening；
The aqueous dispersions are poured into the fermentation base-material, viscous paste is uniformly mixing to obtain, ferment 15-20 under the conditions of anaerobism is protected from light
It, obtains the composite bacteria agent.
The weight proportion of above-mentioned preparation method is but the system of the composite bacteria agent on the basis of the leavening of 1 parts by weight
It is standby to can be as small as bench scale (the lab scale managemental experiment for blue algae polluted water body), pilot-scale (for blue algae polluted water body
Pilot scale managemental experiment) (extensive administer blue algae polluted water body for being commercialized) until commercial scale, therefore, the leavening and
The weight of rice straw powder, reed powder and rice husk powder, which can according to need, to be scaling up.
The mixing of rice straw powder, reed powder and rice husk powder can carry out in any suitable container, can manually stir
Mixing, can also be stirred with machine, obtain fermentation base-material as long as being uniformly mixed.The leavening is dispersed in suitable weight
It measures in the water of ratio and forms aqueous dispersions.Preferably, with a large amount of water dispersion leavening, for example, the weight of water and the dispersing agent
It is (calcium lime powder, red so that each ingredient of the culture medium of the leavening than that can be 10:1,20:1,50:1 even 100:1 or higher
Sugar, sodium bicarbonate, edible salt, yeast powder, rice straw powder, plant ash and vanadic anhydride) concentration more dilute, reduce its concentration pair
The stress of molten algae dominant strain influences.On the other hand, the dosage of water will be controlled stirs in resulting aqueous dispersions and the fermentation base-material
Mix uniformly after obtain viscous paste, without apparent water exudation subject to.
Fermentation 15-20 days is carried out under the conditions of anaerobism is protected from light.Anaerobism is protected from light condition primarily to promoting rice straw powder, reed
Powder and rice husk powder per se with lactic acid bacteria proliferation, it is preferable that carried out in the sealed fermenting room that constant temperature and humidity anaerobism is protected from light
Fermentation, at 35-37 DEG C, relative humidity is controlled in 80-90% for temperature control.Closed fermenting cellar can be to avoid the pollution of miscellaneous bacteria.
The present inventor has developed medium component calcium lime powder, brown sugar, sodium bicarbonate, food in leavening of the invention meticulously
With salt, yeast powder, rice straw powder, plant ash and vanadic anhydride.They are played in the preservation of leavening stablizes molten algae dominant bacteria
The effect of strain freeze-dried powder；Play the role of the molten algae dominant strain of activation when leavening is configured to aqueous dispersions with water；And
It is molten algae dominant strain when progress anaerobism is protected from light fermentation after mixing the aqueous dispersions with rice straw powder, reed powder and rice husk powder
With lactobacter growth breeding provide necessary inorganic salts (such as the calcium ion from calcium lime powder, the sodium ion from edible salt,
The different kinds of ions such as the potassium ion from plant ash), vitamin (such as from yeast powder), alkaline environment (such as carry out consisting of quicklime
Powder, sodium bicarbonate, plant ash).
It is worth mentioning that this ingredient of vanadic anhydride.Vanadic anhydride itself has toxicity, but the present inventor is in length
It is found in the scientific research and practice of phase, the dosage of vanadic anhydride controls in a small amount of range, has stablize and activate micro- life instead
The effect of object bacterial strain.Therefore, vanadic anhydride can stablize molten algae dominant strain freeze-dried powder in leavening of the invention, rear
Continuous anaerobism, which is protected from light fermentation, can activate molten algae dominant strain and lactic acid bacteria, have to leavening and composite bacteria agent of the invention and close
The effect of key.Since vanadic anhydride has toxic effect to microorganism, so the amount of vanadic anhydride cannot in leavening
Excessively, molten algae dominant strain excessively can be then poisoned, can not be very little, do not have the stable and molten algae dominant strain of activation energy then very little
With the effect of lactic acid bacteria.In leavening of the invention, based on the cyanobacteria dominant strain freeze-dried powder of 1 parts by weight, vanadic anhydride
Amount be 0.1-0.5 parts by weight, preferably 0.2-0.4 parts by weight, most preferably 0.3 parts by weight.According to above for composite bacteria agent
The description of the weight ratio and the water about aqueous dispersions of each ingredient (leavening, rice straw powder, reed powder and rice husk powder), in reality
Border fermentation in, the amount of vanadic anhydride be it is very low, be avoided that the toxic effect of vanadic anhydride, and can activate and promote molten algae
The growth of dominant strain and lactic acid bacteria.
Furthermore it is preferred that being then added to rice straw powder, reed powder and paddy after standing 5-10 minutes after preparing aqueous dispersions
In shell powder, so that molten algae dominant strain freeze-dried powder rehydration, resurrection in leavening, and pass through higher concentration in aqueous dispersions
Vanadic anhydride (relative to aqueous dispersions and rice straw powder, reed powder and the mixed concentration of rice husk powder) molten algae advantage of initial activation
Bacterial strain.
Rice straw powder used in the fermentation of mix bacterium agent of the invention, reed powder and rice husk powder are the present inventor from a large amount of
The optimal raw material that science is detected, compared, filtering out in raw material.Rice straw powder, reed powder and rice husk powder sugar rich in, naturally
Containing lactic acid bacteria culturers, and they belong to the lower raw material of value, and whole nation major part rural area has flood tide output every year, is easy
It obtains, it is at low cost.Be protected from light in fermentation process in anaerobism, lactic acid bacteria culturers leavening of the invention medium component especially
Activity is improved under the activation of vanadic anhydride rapidly, the sugar of Rice Straw Fermented powder, reed powder and rice husk powder, mass propagation becomes
Advantage lactobacillus.Meanwhile being protected from light in fermentation process in anaerobism, rice straw powder, reed powder and rice husk powder also promote molten algae dominant bacteria
The fermentation proliferation of strain.
The mix bacterium agent of the invention obtained after fermentation is a kind of using rice straw powder, reed powder and rice husk powder as the molten of substrate
Algae dominant bacteria and lactic acid bacteria mix bacterium agent.Moist microbial inoculum product is vacuum-packed into, can be saved at normal temperature, is saved for a long time
Then preferably saved in freezer.The product can be applied to administer blue algae polluted water body.
Therefore, the fifth aspect of the present invention provides the compound bacteria of the leavening especially third aspect of first aspect present invention
Agent is used to administer the purposes of blue algae polluted water body.Molten algae dominant strain in the leavening or the composite bacteria agent can be in water body
The active material for growing and secreting inhibition, kill cyanobacteria, promotes Cells of Blue-green Algae apoptosis.A large amount of lactic acid bacteria energy in the composite bacteria agent
The organic substances such as carbohydrate, the cellulose grow and adsorb in water body, degrade, converting, being discharged using dead Cells of Blue-green Algae,
These substances are reduced to the eutrophication pollution of water body.And the lactic acid bacteria of raised growth can be by primary dynamic in water body in water body
Object and fishes and shrimps are ingested.Therefore, application of the composite bacteria agent through the invention in water body, make the cyanobacteria of blue algae polluted water body by
Inhibit, maintains beneficial microbe and the aquatic organism ecological balance in water body, it is long-term to keep the limpid pollution-free of water body.
Correspondingly, the sixth aspect of the present invention provides a kind of method for administering blue algae polluted water body, and this method includes that incite somebody to action this
The composite bacteria agent of the third aspect of invention puts into blue algae polluted water body to be administered.In general, based on 1 meter of mean depth, it is blue per acre
Described 15-20 kilograms of composite bacteria agent of algae pollution water body investment.Generally, it chlorophyll occurs pollutes to be formed but do not generate indigo plant in water body
When algae wawter bloom phenomenon, in order to inhibit blue algae growth, based on 1 meter of mean depth, blue algae polluted water body puts into the compound bacteria per acre
10-15 kilograms of agent；After generating cyanobacterial bloom phenomenon, based on 1 meter of mean depth, blue algae polluted water body investment is described compound per acre
20-30 kilograms of microbial inoculum.
Blue algae polluted water body is usually poisons in freshwater, it is also possible to including salt water water body (such as ocean water body).Use of the invention
Poisons in freshwater is particularly suitable in the composite bacteria agent for administering blue algae polluted water body and the method for administering blue algae polluted water body, such as by
River, lake, pond, reservoir, the landscape water body etc. of cyanobacteria pollution.
For the broad applicability for improving the method for administering blue algae polluted water body of the invention, usually use of the invention is being prepared
When administering the leavening of blue algae polluted water body, blue algae polluted water body environment (such as river, lake, the pond different from least three kinds
Pool environment) in screening obtain at least three batches of molten algae dominant strains and be prepared into three batches of leavenings, then using the four directions of the present invention
Method described in face is prepared at least three batches for administering the compound strain of blue algae polluted water body.In practical application, according to not
The environment of same blue algae polluted water body, at least three batches of compound strains can be used alone for this, it is also possible to make by different proportion combination
With.
Below by way of specific embodiment, the present invention will be described in detail.It should be appreciated that these embodiments are only exemplary
, it does not constitute a limitation on the scope of protection of the present invention.
Embodiment 1: for administering the preparation of the leavening of blue algae polluted water body
The leavening for administering blue algae polluted water body is prepared according to the following steps.
Step 1. screens molten algae dominant strain
Acquire blue algae polluted water body sample respectively in the river water, water body in lake and pond waters polluted by cyanobacteria,
Screening obtains three batches of molten algae dominant strains as follows, and is prepared into three batches of molten algae dominant strain freeze-dried powders.
Screen the screening technique of molten algae dominant strain:
(1) blue algae polluted water body sample acquires: selecting typical blue algae polluted water body, acquisition water sample, which is fitted into plastic bottle, to be protected
It deposits.
(2) water sample pre-processes: taking a small amount of water sample, first with 0.8 μm of Fibrous membrane filtration, gained filtrate uses 0.22 μm again
Fibrous membrane filtration is collected 0.22 μm of tunica fibrosa, is rinsed with deionized water, prepares the bacterium solution containing potential molten algae dominant strain, sets
It is saved in triangular flask.
(3) potential molten algae dominant strain culture: molten algae dominant strain culture medium (NH is prepared4H2PO4 1g,MgSO4·7H2O
0.2g, trisodium citrate, 0.5g, K2HPO41g, agar 20g, distilled water 1000mL), after autoclave sterilization, inverted plate.
The bacterium solution being prepared is diluted to the bacterium solution for being prepared into 5 dilutions by 10 times, is respectively coated plate, is inverted in 30 DEG C of biochemical trainings
Support case culture for 24 hours.The suitable plate of selecting bacteria stand density supplies subsequent picking single colonie.
(4) potential molten algae dominant strain screening: the water sample containing cyanobacteria of acquisition is sub-packed in 250ml triangular flask, every bottle
100ml.From plate picking single colonie, it is inoculated in a triangular flask respectively.Triangular flask is put on rotary shaker, 30 DEG C,
It is cultivated 1 day under 60rpm.One or several triangular flasks that obvious yellow, molten algae occur for cyanobacteria are selected, by above-mentioned " water sample pretreatment "
Method prepare the bacterium solution containing potential molten algae dominant strain, then obtained by above-mentioned " potential molten algae dominant strain culture " method single
Bacterium colony, as required molten algae dominant strain.It should be noted that molten algae dominant strain single colonie obtained may be endless
Pure bacterial strain entirely or several single colonie distributions obtained belong to different molten algae dominant strains, but they all have it is molten
The effect of algae is all the molten algae dominant strain for meeting the present invention and needing.
Step 2. prepares starter medium mixture
Raw material preparation: brown sugar, sodium bicarbonate, edible salt, yeast powder are bought from department stores；Chemically reagent shop purchase life
Pulverized limestone and vanadic anhydride；The powder of 1mm or partial size below are ground into through pulverizer using dry rice straw, obtains rice straw powder；
Ashes are taken using dry faggot burning, obtain plant ash.
Ingredient: weigh 10 parts by weight of calcium lime powder, 20 parts by weight of brown sugar, 20 parts by weight of sodium bicarbonate, 40 parts by weight of edible salt,
40 parts by weight of yeast powder, 80 parts by weight of rice straw powder, 20 parts by weight of plant ash, 0.1 parts by weight of vanadic anhydride；In addition to saccharomycete,
Each ingredient is ground using mortar, so that each ingredient crushes and is mixed to get homogeneous mixture；Then it is slight to add yeast
It stirs evenly to avoid damage saccharomycete, obtains culture medium mixture A.
In addition weigh 20 parts by weight of calcium lime powder, 30 parts by weight of brown sugar, 30 parts by weight of sodium bicarbonate, 50 parts by weight of edible salt,
50 parts by weight of yeast powder, 100 parts by weight of rice straw powder, 30 parts by weight of plant ash, 0.3 parts by weight of vanadic anhydride；In addition to saccharomycete,
Each ingredient is ground using mortar, so that each ingredient crushes and is mixed to get homogeneous mixture；Then it is slight to add yeast
It stirs evenly to avoid damage saccharomycete, obtains culture medium mixture B.
Still further weigh 30 parts by weight of calcium lime powder, 40 parts by weight of brown sugar, 40 parts by weight of sodium bicarbonate, 60 weight of edible salt
Part, 60 parts by weight of yeast powder, 120 parts by weight of rice straw powder, 40 parts by weight of plant ash, 0.5 parts by weight of vanadic anhydride；Except saccharomycete
Outside, each ingredient is ground using mortar, so that each ingredient crushes and is mixed to get homogeneous mixture；Then it is light to add yeast
It is micro- to stir evenly to avoid damage saccharomycete, obtain culture medium mixture C.
Step 3. prepares the leavening for administering blue algae polluted water body
First molten 1 parts by weight of algae dominant strain freeze-dried powder prepared in step 1 are weighed, the training of step 2 preparation is added to
It supports in based mixtures A, is softly mixed thoroughly with spoon in order to avoid damaging strain cell, leavening A is made.
In addition molten 1 parts by weight of algae dominant strain freeze-dried powder of the second batch prepared in step 1 are weighed, step 2 preparation is added to
Culture medium mixture B in, softly mixed thoroughly with spoon in order to avoid damage strain cell, leavening B be made.
Molten 1 parts by weight of algae dominant strain freeze-dried powder of third batch prepared in step 1 are still further weighed, step 2 system is added to
In standby culture medium mixture C, is softly mixed thoroughly with spoon in order to avoid damaging strain cell, leavening C is made.
Leavening A, B and C sealing preservation obtained is spare in 4 DEG C of refrigerators.
Embodiment 2: for administering the preparation of the mix bacterium agent of blue algae polluted water body
The mix bacterium agent for administering blue algae polluted water body is prepared according to the following steps.
Step 1: prepare fermentation base-material:
Raw material preparation: acquiring straw, reed, husk in field farmland, is dried to moisture content 10% hereinafter, with crushing
Machine is ground into the powder of 1mm or partial size below.
Ingredient: weighing 60 parts by weight of grass meal, 15 parts by weight of reed powder, 10 parts by weight of rice husk powder, is uniformly mixed and is fermented
Base material A.
In addition 65 parts by weight of grass meal, 20 parts by weight of reed powder, 15 parts by weight of rice husk powder are weighed, is uniformly mixed and obtains fermentation base
Expect B.
70 parts by weight of grass meal, 25 parts by weight of reed powder, 20 parts by weight of rice husk powder are still further weighed, is uniformly mixed and is fermented
Base-material C.
Step 2: preparing leavening aqueous dispersions
Weigh embodiment 1 preparation each 1 parts by weight of leavening A, B and C, be scattered in respectively 20 parts by weight, 50 parts by weight and
In the tap water of 100 parts by weight, it is configured to leavening aqueous dispersions A, B and C.
Step 3: preparing the mix bacterium agent for administering blue algae polluted water body
Step 2 is prepared into obtained leavening aqueous dispersions A, B and C and pours into the fermentation base-material that step 1 preparation obtains respectively
A, B and C, stirs evenly, the fermentation being protected from light respectively in the constant temperature and humidity anaerobism that temperature is 37-38 DEG C, relative humidity is 80-90%
Indoor fermentation 15-20 days respectively obtains mix bacterium agent A, B and C for administering blue algae polluted water body.
Embodiment 3: landscape pond cyanobacteria sewage water body treating test
It chooses the landscape pond that chlorophyll is seriously polluted at Guangdong one and carries out managemental experiment.About 3 mu of the pond water surface, the depth of water
0.6-1.3 meters, 1 meter of mean depth.Due to the inflow of around sanitary sewage, pond eutrophication is caused, existing cyanobacteria grows, leaf
Green element reaches 1000mg/L, has sign to generate cyanobacterial bloom phenomenon.
Cyanobacteria breeds growth in order to prevent and the water surface further pollutes, and shuts off first to sanitary sewage, avoids flowing again
Enter the pond.Then, chlorophyll is substantially eliminated after totally 50 kilograms, 1 day of mix bacterium agent A of the preparation of embodiment 2 are launched to the pond
Phenomenon, and other pernicious bacterias are killed, water body becomes clear.Hereafter range estimation water body is always maintained at limpid weekly, and monthly sample detection is hard
Half a year is held, which remains the advantage of profitable strain, has no other pollutions, and pond aquatic microbio-ecology is made to reach balance
State, and keep for a long time.
Embodiment 4: cultivating pool cyanobacteria sewage water body treating test
It chooses the cultivating pool polluted at Anhui one by cyanobacteria and carries out managemental experiment.About 2 mu of the cultivating pool water surface, it is average
1 meter of the depth of water, due to high-density breeding fish, feed causes water eutrophication plus the excreta of fish, and cyanobacteria grows.
In order to inhibit the growth of cyanobacteria, prepared by embodiment 2 totally 40 kilograms of mix bacterium agent B are launched to the pond, after a few days
Cyanobacteria is subsided, and cyanobacteria disappears substantially after a week, and water transparency improves.Hereafter the situation of change of observation water body, one month
Find that the excreta that feed and fish are constantly put into due to cultured fishes is constantly accumulated afterwards, water body greening, and have generation cyanobacterial bloom
Sign.Put into totally 30 kilograms of mix bacterium agent A of the preparation of embodiment 2 again in time, water body becomes clear again after a few days.Hereafter there is hair again
It is equally handled when the sign of raw cyanobacterial bloom, breeding water body totally keeps the state that cyanobacterial bloom does not occur substantially.
Embodiment 5: lake blue algae sewage water body treating test
It chooses the park lake polluted at Jiangxi one by cyanobacteria and carries out reason test.About 10 mu of the lake, mean depth
About 1.5 meters.Due to the inflow of surrounding resident and restaurant sewage, serious blue algae bloom has occurred in summer.
To administer lake blue algae, prepared by embodiment 2 totally 450 kilograms of mix bacterium agent C, week more than one are launched to the lake
Cyanobacteria has the sign of recession afterwards, and cyanobacteria is largely subsided after one month, but due to the continuous inflow of sanitary sewage, cyanobacteria still no
To elimination.Hereafter the 1:1:1 for launching mix bacterium agent A, B and C prepared by embodiment 2 every month blends totally 300 kilograms of microbial inoculum, lake
Water body cyanobacteria greatly inhibited, be not evident that cyanobacteria, but lake water still have it is a little green tinged, this be sanitary sewage not
It stops caused by caused a small amount of blue algae growth.The present inventor suggests that park seeks government department and carries out interception project, from source
Head eradicates cyanobacteria pollution, can save the spending using mix bacterium agent.
Embodiment 6: river cyanobacteria sewage water body treating test
It is to have that above embodiments 3-5, which demonstrates pond, the lake that composite bacteria agent of the invention is polluted improvement by cyanobacteria,
Effect.The present embodiment is tested in the river polluted by cyanobacteria.Due to the flowing of river water, test effect is not so good as in water flow phase
It is so obvious to static pond or lake.But it can be according to flow velocity and the flow estimation composite bacteria agent of the present invention of river water
Injected volume, the appropriate input amount for increasing composite bacteria agent, composite bacteria agent inhibits the cyanobacteria in downstream with water body flow to downstream.Such as
Having on fruit still, which has cyanobacteria to flow through, comes, then periodically launches composite bacteria agent as needed.The present embodiment is shown for cyanobacteria of harnessing a river
It is effective.
Use above specific example is expounded the present invention, is merely used to help understand the present invention, not to
The limitation present invention.The design of those skilled in the art according to the present invention can also be made and several simply push away
It drills, deform or replaces.These are deduced, deformation or alternative are also fallen into scope of the presently claimed invention.
Claims (10)
1. a kind of for administering the leavening of blue algae polluted water body, which is characterized in that the following component including following parts by weight: from
Molten 1 part of algae dominant strain freeze-dried powder, 10-30 parts of calcium lime powder, 20-40 parts of the brown sugar, sodium bicarbonate screened in blue algae polluted water body
20-40 parts, 40-60 parts of edible salt, 40-60 parts of yeast powder, 80-120 parts of rice straw powder, 20-40 parts of plant ash, vanadic anhydride
0.1-0.5 parts.
2. leavening according to claim 1, which is characterized in that the following component including following parts by weight: cyanobacteria pollution
Molten 1 part of the algae dominant strain freeze-dried powder that is screened in water body, 15-25 parts of calcium lime powder, 25-35 parts of brown sugar, 25-35 parts of sodium bicarbonate,
45-55 parts of edible salt, 45-55 parts of yeast powder, 90-110 parts of rice straw powder, 25-35 parts of plant ash, 0.2-0.4 parts of vanadic anhydride.
3. leavening according to claim 2, which is characterized in that the following component including following parts by weight: cyanobacteria pollution
Molten 1 part of algae dominant strain freeze-dried powder, 20 parts of calcium lime powder, 30 parts of brown sugar, 30 parts of the sodium bicarbonate, edible salt 50 screened in water body
Part, 50 parts of yeast powder, 100 parts of rice straw powder, 30 parts of plant ash, 0.3 part of vanadic anhydride.
4. a kind of method for preparing leavening according to any one of claim 1-3, which is characterized in that including following step
It is rapid:
Molten algae dominant strain is screened from blue algae polluted water body, and is prepared into bacterial strain freeze-dried powder；
Calcium lime powder, brown sugar, sodium bicarbonate, edible salt, yeast powder, rice straw powder, plant ash, five oxygen are weighed by the weight ratio
Change two vanadium, is uniformly mixed and obtains mixture；
It takes 1 parts by weight of bacterial strain freeze-dried powder to mix with the mixture, obtains the leavening.
5. a kind of for administering the composite bacteria agent of blue algae polluted water body, which is characterized in that the following component including following parts by weight:
1 part of leavening according to any one of claim 1-3,60-70 parts of rice straw powder, 15-25 parts of reed powder, rice husk powder 10-
20 parts, by the way that the ingredient is fermented under the conditions of anaerobism is protected from light.
6. composite bacteria agent according to claim 5, which is characterized in that the following component including following parts by weight: according to power
Benefit requires 1 part of leavening, 65 parts of rice straw powder described in any one of 1-3,20 parts of reed powder, 15 parts of rice husk powder, by will be described
Ingredient ferments under the conditions of anaerobism is protected from light.
7. a kind of method for preparing composite bacteria agent according to claim 5 or 6, which comprises the following steps:
Rice straw powder, reed powder and rice husk powder are weighed by the weight ratio, is uniformly mixed and obtains fermentation base-material；
The leavening for taking 1 parts by weight is dispersed in water to obtain the aqueous dispersions of the leavening；
The aqueous dispersions are poured into the fermentation base-material, are stirred evenly, ferments 15-20 days, obtains under the conditions of anaerobism is protected from light
To the composite bacteria agent.
8. leavening according to any one of claim 1-3 is answered according to any one of claim 5-6
Combined bacteria agent is used to administer the purposes of blue algae polluted water body.
9. a kind of method for administering blue algae polluted water body, which is characterized in that will be answered according to any one of claim 5-6
Combined bacteria agent puts into blue algae polluted water body to be administered.
10. according to the method described in claim 9, it is characterized in that, blue algae polluted water body is thrown per acre based on 1 meter of mean depth
Enter 15-20 kilograms of the composite bacteria agent.
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| CN112410267A (en) * | 2020-12-07 | 2021-02-26 | 武汉市鄂正农科技发展有限公司 | Microbial inoculum for treating cyanobacterial bloom and preparation method thereof |
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