CN109276485B - Acne removing patch and preparation method thereof - Google Patents

Abstract

The invention discloses an acne removing patch and a preparation method thereof, belonging to the technical field of cosmetics, wherein the acne removing patch consists of a patch and an inclusion solidified on the patch, wherein the inclusion consists of the following components in percentage by mass: 5-90% of pichia pastoris fermentation lysate filtrate, 5-90% of glycerol, 0.5-1% of p-hydroxyacetophenone, 0.5-1% of 1,2 hexanediol, 0-1% of hydroxyethyl fiber and the balance of water. The formula and the preparation method of the acne removing patch supplement each other, and the acne removing patch can remarkably improve the efficacy of acne removal.

Description

Acne removing patch and preparation method thereof

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to an acne removing patch and a preparation method thereof.

Background

The acne removing patch is a new patch developed by research personnel, and has the main advantages of safety, effectiveness and convenience for local use. The patch shapes of the acne removing patch are different, so that the acne removing patch is very attractive to consumers. The traditional acne removal patch mainly comprises traditional Chinese medicine composite components and an antiseptic bactericide as main components, wherein a part of acne removal patches are mainly developed by using a patch material, and the material can prevent infection.

However, the existing acne removal patches are to be further improved in the aspect of acne removal efficacy. Therefore, there is a need to design a new acne removing patch to improve the efficacy of acne removal.

Disclosure of Invention

The invention aims to overcome the defects in the prior art and provides the acne removing patch and the preparation method thereof.

In order to achieve the purpose, the invention adopts the technical scheme that: the acne removing paste consists of a patch and an inclusion solidified on the patch, wherein the inclusion consists of the following components in percentage by mass: 5-90% of pichia pastoris fermentation lysate filtrate, 5-90% of glycerol, 0.5-1% of p-hydroxyacetophenone, 0.5-1% of 1,2 hexanediol, 0-1% of hydroxyethyl fiber and the balance of water.

The pichia pastoris fermentation lysate filtrate is a lysate filtrate extracted after fermentation by pichia pastoris under specific conditions, and has strong microbial inhibitory ability, such as acnes; it also has effects in promoting wound healing, preventing scar, promoting hyaluronic acid synthesis, repairing skin barrier, and enhancing immunity. Currently, pichia pastoris fermentation lysate filtrate is used only for whitening in cosmetics. Glycerol is colorless, odorless, sweet, clear, viscous liquid, and has effects of keeping moisture, resisting oxidation, and promoting alcoholization. P-hydroxyacetophenone is one of novel cosmetic preservatives, has good preservative effect and high safety, is not in the limited preservative catalogue of the technical Specification for cosmetic safety 2015 edition per se, and is very preferred by consumers and manufacturers. 1, 2-hexanediol is one of novel cosmetic preservatives, has good preservative effect and high safety, is not in a limited preservative catalogue of cosmetic safety technical specification 2015 edition per se, and is very preferred by consumers and manufacturers. Hydroxyethyl cellulose (HEC) is a white or light yellow, tasteless and nontoxic fibrous or powdery solid, which can be prepared by etherification of alkaline cellulose and ethylene oxide (or chloroethanol), and has the characteristics of good thickening, suspension, dispersion, emulsification, adhesion, film formation, moisture protection, protective colloid and the like.

The inclusion of the acne-removing patch consists of the pichia pastoris fermentation lysate filtrate, glycerol, p-hydroxyacetophenone, 1, 2-hexanedioic acid and hydroxyethyl fiber (or no hydroxyethyl fiber exists), and the pichia pastoris fermentation lysate filtrate is unexpectedly found to be capable of promoting wound healing, preventing scars, repairing skin barriers and improving immunity, has different effects of whitening and the like compared with the leavening known by consumers, and is very suitable for the acne-removing patch; in addition, the prepared acne removing patch can be conveniently used locally, and the retention time is prolonged so as to exert good efficacy.

As an improvement of the technical scheme, the inclusion comprises the following components in percentage by mass: pichia pastoris fermentation lysate filtrate 10%, glycerol 5%, p-hydroxyacetophenone 0.5%, 1,2 hexanediol 0.5%, and hydroxyethyl fiber 0.1%, with the balance being water.

As an improvement of the technical scheme, the inclusion comprises the following components in percentage by mass: pichia pastoris fermentation lysate filtrate 15%, glycerol 10%, p-hydroxyacetophenone 1%, 1,2 hexanediol 1%, and hydroxyethyl fiber 0, with the balance being water.

As an improvement of the technical scheme, the inclusion comprises the following components in percentage by mass: the fermentation lysate filtrate of pichia pastoris is 20%, glycerol is 20%, p-hydroxyacetophenone is 1%, 1,2 hexanediol is 1%, hydroxyethyl fiber is 0.5%, and the balance is water.

The content of the acne paste is limited, and the acne paste prepared by the 3 formulas has better acne removing effect.

In addition, the invention also provides a preparation method of the acne removing paste, which comprises the following steps:

s1) adding water into a reaction kettle, adding glycerol, hydroxyethyl cellulose and p-hydroxyacetophenone in sequence under homogenization, stirring and heating to 80-85 ℃, and keeping the temperature for 30 minutes;

s2), stirring and cooling to 35 ℃, then adding 1, 2-hexanediol and Pichia pastoris fermentation lysate filtrate, stirring uniformly, then sieving with a 200-mesh sieve, and discharging to obtain inclusion;

s3) selecting a proper patch, filling the patch and the content into an aluminum film bag, and sterilizing for later use; and curing the sterilized contents on the sterilized patch, and then sequentially sealing, spraying codes and packaging.

The invention has the beneficial effects that: the invention provides an acne removing patch and a preparation method thereof, the inclusion of the acne removing patch consists of a Pichia pastoris fermentation lysate filtrate, glycerol, p-hydroxyacetophenone, 1, 2-hexanedi and hydroxyethyl fiber, and the Pichia pastoris fermentation lysate filtrate is unexpectedly found to promote wound healing, prevent scars, repair skin barriers and improve immunity; in the preparation process of the acne removing patch, the adding sequence and temperature control of materials are strictly limited, so that the active ingredients of the Pichia pastoris fermentation lysate filtrate are ensured to have activity, and all the components are fully and uniformly mixed.

Detailed Description

To better illustrate the objects, aspects and advantages of the present invention, the present invention will be further described with reference to specific examples.

Example 1

The embodiment provides an acne removal patch, which consists of a patch and an inclusion solidified on the patch, wherein the inclusion consists of the following components in percentage by mass: pichia pastoris fermentation lysate filtrate 10%, glycerol 5%, p-hydroxyacetophenone 0.5%, 1,2 hexanediol 0.5%, and hydroxyethyl fiber 0.1%, with the balance being water.

In addition, the preparation method of the acne removing patch comprises the following steps:

s1) adding water into a reaction kettle, adding glycerol, hydroxyethyl cellulose and p-hydroxyacetophenone in sequence under homogenization, stirring and heating to 80-85 ℃, and keeping the temperature for 30 minutes;

s2), stirring and cooling to 35 ℃, then adding 1, 2-hexanediol and Pichia pastoris fermentation lysate filtrate, stirring uniformly, then sieving with a 200-mesh sieve, and discharging to obtain inclusion;

s3) selecting a proper patch, filling the patch and the content into an aluminum film bag, and sterilizing for later use; and curing the sterilized contents on the sterilized patch, and then sequentially sealing, spraying codes and packaging.

Example 2

The embodiment provides an acne removal patch, which consists of a patch and an inclusion solidified on the patch, wherein the inclusion consists of the following components in percentage by mass: pichia pastoris fermentation lysate filtrate 15%, glycerol 10%, p-hydroxyacetophenone 1%, 1,2 hexanediol 1%, and hydroxyethyl fiber 0, with the balance being water.

In addition, the preparation method of the acne removing patch comprises the following steps:

s1) adding water into a reaction kettle, adding glycerol, hydroxyethyl cellulose and p-hydroxyacetophenone in sequence under homogenization, stirring and heating to 80-85 ℃, and keeping the temperature for 30 minutes;

s2), stirring and cooling to 35 ℃, then adding 1, 2-hexanediol and Pichia pastoris fermentation lysate filtrate, stirring uniformly, then sieving with a 200-mesh sieve, and discharging to obtain inclusion;

s3) selecting a proper patch, filling the patch and the content into an aluminum film bag, and sterilizing for later use; and curing the sterilized contents on the sterilized patch, and then sequentially sealing, spraying codes and packaging.

Example 3

The embodiment provides an acne removal patch, which consists of a patch and an inclusion solidified on the patch, wherein the inclusion consists of the following components in percentage by mass: the fermentation lysate filtrate of pichia pastoris is 20%, glycerol is 20%, p-hydroxyacetophenone is 1%, 1,2 hexanediol is 1%, hydroxyethyl fiber is 0.5%, and the balance is water.

In addition, the preparation method of the acne removing patch comprises the following steps:

s1) adding water into a reaction kettle, adding glycerol, hydroxyethyl cellulose and p-hydroxyacetophenone in sequence under homogenization, stirring and heating to 80-85 ℃, and keeping the temperature for 30 minutes;

s2), stirring and cooling to 35 ℃, then adding 1, 2-hexanediol and Pichia pastoris fermentation lysate filtrate, stirring uniformly, then sieving with a 200-mesh sieve, and discharging to obtain inclusion;

s3) selecting a proper patch, filling the patch and the content into an aluminum film bag, and sterilizing for later use; and curing the sterilized contents on the sterilized patch, and then sequentially sealing, spraying codes and packaging.

Example 4

The embodiment provides an acne removal patch, which consists of a patch and an inclusion solidified on the patch, wherein the inclusion consists of the following components in percentage by mass: pichia pastoris fermentation lysate filtrate 5%, glycerol 90%, p-hydroxyacetophenone 0.7%, 1,2 hexanediol 0.8%, and hydroxyethyl fiber 1%, with the balance being water.

In addition, the preparation method of the acne removing patch comprises the following steps:

s1) adding water into a reaction kettle, adding glycerol, hydroxyethyl cellulose and p-hydroxyacetophenone in sequence under homogenization, stirring and heating to 80-85 ℃, and keeping the temperature for 30 minutes;

s2), stirring and cooling to 35 ℃, then adding 1, 2-hexanediol and Pichia pastoris fermentation lysate filtrate, stirring uniformly, then sieving with a 200-mesh sieve, and discharging to obtain inclusion;

s3) selecting a proper patch, filling the patch and the content into an aluminum film bag, and sterilizing for later use; and curing the sterilized contents on the sterilized patch, and then sequentially sealing, spraying codes and packaging.

Example 5

The embodiment provides an acne removal patch, which consists of a patch and an inclusion solidified on the patch, wherein the inclusion consists of the following components in percentage by mass: pichia pastoris fermentation lysate filtrate 90%, glycerol 6%, p-hydroxyacetophenone 0.5%, 1,2 hexanediol 0.5%, and hydroxyethyl fiber 0.5%, the balance being water.

In addition, the preparation method of the acne removing patch comprises the following steps:

s1) adding water into a reaction kettle, adding glycerol, hydroxyethyl cellulose and p-hydroxyacetophenone in sequence under homogenization, stirring and heating to 80-85 ℃, and keeping the temperature for 30 minutes;

s2), stirring and cooling to 35 ℃, then adding 1, 2-hexanediol and Pichia pastoris fermentation lysate filtrate, stirring uniformly, then sieving with a 200-mesh sieve, and discharging to obtain inclusion;

s3) selecting a proper patch, filling the patch and the content into an aluminum film bag, and sterilizing for later use; and curing the sterilized contents on the sterilized patch, and then sequentially sealing, spraying codes and packaging.

Comparative example 1

The comparison example provides an acne removal patch, which is different from the acne removal patch in example 1 in that: the contents were free of pichia pastoris fermentation lysate filtrate.

Comparative example 2

The comparison example provides an acne removal patch, which is different from the acne removal patch in example 2 in that: the contents were free of pichia pastoris fermentation lysate filtrate.

Comparative example 3

The comparative example provides an acne removal patch, which is different from the acne removal patch in example 3 in that: the contents were free of pichia pastoris fermentation lysate filtrate.

Effect example 1

The pichia pastoris fermentation lysate filtrate of the present invention was subjected to an antibacterial experiment, and the strain was propionibacterium acnes, and the results are shown in table 1.

TABLE 1

As shown in Table 1, the Pichia pastoris fermentation lysate filtrate added into the acne removing patch has good bacteriostatic effect and can inhibit the growth of bacteria in acne.

Effect example 2

The acne removal patches of examples 1-3 are used as research objects; selecting 60 subjects with normal skin in half of male and female; the groups were divided into 3 groups of 10 men and women. Covering the anti-acne paste on the part of the arm of the testee, removing the anti-acne paste for 24 hours, observing the skin reaction after removing for 0.5, 12, 24 and 48 hours respectively, and recording the result according to the skin grading standard in the technical Specification for cosmetic safety 2015 edition.

The result of the human body skin patch experiment shows that: the acne removal patches of examples 1-3 are applied to each group of subjects, and no adverse skin reaction occurs after 0.5, 12, 24 and 48 hours, which indicates that the acne removal patches are safe to use.

Effect example 3

The acne removal patches of examples 1-3 and comparative examples 1-3 are used as research objects; 120 subjects are selected, the age of each subject is 20-40, the face of each subject has obvious acne and pox, no allergic history of the skin disease, and the subject accords with the volunteer selection standard. The test method comprises the following steps: selecting the region with acne, and applying the acne-removing paste on the cleaned affected part for 1 time in the morning and evening every day for 2 weeks; the subjects kept good daily rest time and dietary habits during the trial period; counting the feedback condition of the subjects after 2 weeks; the results are shown in Table 2.

TABLE 2

As shown in table 2, the acne removing patch of the present invention can well remove acne, repair skin and repair acne marks, i.e., improve redness and swelling of skin, exuberance of oil secretion and smooth skin.

The above lists only the effect examples of the acne removal patches in the embodiments 1-3 of the invention, and the acne removal patches in other embodiments of the invention can also achieve similar acne removal effects. If the content of the acne removing paste consists of the following components in percentage by mass: 5-90% of pichia pastoris fermentation lysate filtrate, 5-90% of glycerol, 0.5-1% of p-hydroxyacetophenone, 0.5-1% of 1,2 hexanediol, 0-1% of hydroxyethyl fiber and the balance of water; the acne removal patch can well remove acnes and repair acnes.

Finally, it should be noted that the above embodiments are intended to illustrate the technical solutions of the present invention and not to limit the scope of the present invention, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications and equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.

Claims (5)

1. The acne removing patch is composed of a patch and an inclusion solidified on the patch, and is characterized in that the inclusion is composed of the following components in percentage by mass: 5-90% of pichia pastoris fermentation lysate filtrate, 5-90% of glycerol, 0.5-1% of p-hydroxyacetophenone, 0.5-1% of 1,2 hexanediol, 0-1% of hydroxyethyl fiber and the balance of water.

2. The acne removal patch according to claim 1, wherein the inclusions consist of the following components in percentage by mass: pichia pastoris fermentation lysate filtrate 10%, glycerol 5%, p-hydroxyacetophenone 0.5%, 1,2 hexanediol 0.5%, and hydroxyethyl fiber 0.1%, with the balance being water.

3. The acne removal patch according to claim 1, wherein the inclusions consist of the following components in percentage by mass: pichia pastoris fermentation lysate filtrate 15%, glycerol 10%, p-hydroxyacetophenone 1%, 1,2 hexanediol 1%, and hydroxyethyl fiber 0, with the balance being water.

4. The acne removal patch according to claim 1, wherein the inclusions consist of the following components in percentage by mass: the fermentation lysate filtrate of pichia pastoris is 20%, glycerol is 20%, p-hydroxyacetophenone is 1%, 1,2 hexanediol is 1%, hydroxyethyl fiber is 0.5%, and the balance is water.

5. The preparation method of the acne removal patch according to any one of claims 1 to 4, characterized by comprising the following steps:

s1) adding water into a reaction kettle, adding glycerol, hydroxyethyl cellulose and p-hydroxyacetophenone in sequence under homogenization, stirring and heating to 80-85 ℃, and keeping the temperature for 30 minutes;

s2), stirring and cooling to 35 ℃, then adding 1, 2-hexanediol and Pichia pastoris fermentation lysate filtrate, stirring uniformly, then sieving with a 200-mesh sieve, and discharging to obtain inclusion;

s3) selecting a proper patch, filling the patch and the content into an aluminum film bag, and sterilizing for later use; and curing the sterilized contents on the sterilized patch, and then sequentially sealing, spraying codes and packaging.