CN109258930A - A kind of one-step fermentation of dregs of beans, the fermented bean dregs of this method preparation and application - Google Patents
A kind of one-step fermentation of dregs of beans, the fermented bean dregs of this method preparation and application Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
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- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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Abstract
The fermented bean dregs prepared the invention discloses a kind of one-step fermentation of dregs of beans, this method and application, belong to feed fermentation technical field.The method of one-step fermentation dregs of beans of the present invention is that the microorganism sustained release microbial inoculum of material, fermenting agent and zein coating is added at one time fermentor, is fermented.This method can not only shorten the fermentation time of dregs of beans, reduce fermentation process, avoid the pollution of miscellaneous bacteria, moreover it is possible to significantly reduce production cost.Dregs of beans after fermentation can significantly improve animal growth, strengthen immunity, improve digestion and absorption function.
Description
Technical field
The invention belongs to feed fermentation technical fields, and in particular to a kind of one-step fermentation of dregs of beans, this method preparation
Fermented bean dregs and application.
Background technique
Soybean cake dregs are vegetal protein raw material, and crude protein content is higher, reaches 40-50%, it is necessary to amino acid group
At balance, digestibility is high, and the price of deed is high.Wherein, lysine content occupies the umber one in meal expeller and mealsolvent, up to 2.5-3%, still
Methionine content is less, only 0.5-0.7%.In addition, also contain many anti-nutritional factors in soybean cake dregs, and such as: tryptose
Enzyme inhibition factor, goitrogen, the antivitamin factor, phytic acid, urase, soybean antigen, relies the third ammonia at hemagglutinin
Acid, saponin, estrogen, Flatulent factors etc..Their majorities are inactivated during the regular production of soybean cake dregs due to heating, from
And its illeffects is reduced or losed.But heating time is too short, the too low inactivation for being unfavorable for anti-nutritional factors of temperature, and when
Between it is too long, temperature is excessively high, the feeding value of protein can be reduced.
Microbe fermentation method remove dregs of beans in anti-nutritional factors action temperature and, it is highly-safe, removal anti-nutritional factors
While can also degrade macromolecular substances, be conducive to animal digestion absorption；New active material is generated, the growth of animal is promoted to send out
Educate, increase immunity etc.；However, for more thorough removal anti-nutritional factors mixed fungus fermentation can be used mostly, and carry out more
Secondary fermentation；It is not only time-consuming and laborious in this way, increase production cost, and between different fermentations process, bacterium solution or fermentation materials
Addition, it is careless slightly, it also easily causes fermentation materials by living contaminants, fermentation is caused to fail.
Summary of the invention
Aiming at the problems existing in the prior art, the purpose of the present invention is to provide a kind of method of one-step fermentation dregs of beans,
This method is time saving and energy saving, saves production cost, avoids the living contaminants in fermentation process.
In order to achieve the above object, the technical solution of the present invention is as follows:
A kind of microorganism sustained release microbial inoculum of zein coating, is prepared by following methods:
(1) it weighs zein and is mixed with carboxymethyl cellulose by the weight ratio of 5:1, the ethyl alcohol that 5 times of weight 40% are added is molten
In liquid, the glycerol of zein weight 12%, the Tween 80 of zein weight 5% are added；It is uniformly mixed up to coating agent；
(2) microbial bacterial agent is added into coating agent, the additive amount of microbial bacterial agent is the 12% of coating agent weight, closed
Be uniformly mixed rapidly in container, coating liquid made uniformly to be wrapped in the surface of complex micro organism fungicide, be dried to powder to get
The microorganism of zein coating is sustained microbial inoculum.
On the basis of above scheme, the microbial bacterial agent is the active suspend mode of microbial activity bacterium powder or microorganism
Body.
On the basis of above scheme, the microbial bacterial agent is by bacillus cereus, bacillus subtilis and synanthrin
The gemma of lactobacillus is mixed by the mass ratio of 1:1:1.
Application of the microorganism sustained release microbial inoculum of above-mentioned zein coating in animal and fowl fodder fermentation.
A kind of one-step fermentation of dregs of beans, steps are as follows:
(1) pretreatment of raw material: dregs of beans crushed 10 meshes, and 121 DEG C of 30min of high pressure steam sterilization are cooling after the completion of sterilizing
To room temperature；
(2) one-step fermentation: the moisture content for adjusting dregs of beans after sterilizing is 75~85%, and 7~10% meters are inoculated with into dregs of beans
The fermenting microbe of aspergillus and 3~5% aspergillus nigers and 5~8% above-mentioned zein coating complex micro organism fungicide, it is sufficiently mixed
It after conjunction, ferments, 28 DEG C~32 DEG C of fermentation temperature of control keeps moisture content 75~85%, stirring 30~36h of aerobic fermentation
Afterwards, the deionized water of 1 times of amount is added, stops being passed through for oxygen or air, 48~52h of closed stirring fermentation；
(3) it discharges: the hop residue of material gross weight 0.8~1.2% is added, discharges after mixing evenly.
On the basis of above scheme, the fermenting microbe of the aspergillus oryzae and aspergillus niger is prepared by following methods:
It crushes beans shell and crosses 10 meshes, sterilize through 121 DEG C, 15min；Water content 50~65% is adjusted, will be trained through fermented bean drink inclined-plane
Aspergillus oryzae and aspergillus niger strain after supporting base activation are inoculated in beans shell culture medium, 25-30 DEG C of culture 48 by 1% inoculum concentration respectively
Fermenting microbe, strain content >=10 are made in~60h9cfu·g-1Beans shell culture medium.
The dregs of beans of above method fermentation.
Above-mentioned fermented bean dregs improve animal growth, strengthen immunity, improvement digestion and absorption function feed in preparation
In application.
It is a kind of to improve animal growth, strengthen immunity, the feed for improving digestion and absorption function, it is characterised in that:
Above-mentioned fermented bean dregs are added in feed, additive amount is 0.1~0.25kg/kg feed.
The advantages of technical solution of the present invention
The present invention is prepared into coating agent using ingredients such as zein and carboxymethyl celluloses, carries out coating to microbial bacterial agent
Processing, can slow down the release of microbial bacterial agent during the fermentation；The present invention is slow using the microorganism of above-mentioned zein coating
Microbial inoculum is released, a kind of method of one-step fermentation dregs of beans is provided, this method can not only shorten the fermentation time of dregs of beans, reduce fermentation
Process avoids the pollution of miscellaneous bacteria, moreover it is possible to significantly reduce production cost.Dregs of beans after fermentation can significantly improve growth of animal hair
It educates, strengthen immunity, improve digestion and absorption function.
The coating that zein and carboxymethyl cellulose are prepared into the present invention, can wrap up well microbial bacterial agent,
The too fast release of microbial bacterial agent during the fermentation is prevented, the main component of the coating agent is protein component, is easy by albumen
Enzyme degradation, but to stabilized aqueous solution；The addition of glycerol can increase the smoothness of coating, prevent from peeling off.
The microorganism that the method for one-step fermentation dregs of beans of the present invention uses the zein coating of above-mentioned preparation is sustained microbial inoculum；?
Fermentation start when just by the microorganism of each fermenting agent and zein coating sustained release microbial inoculum be added fermentation material in, in fermentation material by
In the stability of coating microbial inoculum, what is carried out at first is the fermentation process of aspergillus niger and aspergillus oryzae, the two growth and breeding, in fermentation point
Larger molecular organics are solved, a variety of extracellular active materials and enzyme, especially protease are generated, it can be by the coating of coating microbial inoculum
It gradually degrades, at this point, the active hypopus of microbial activity bacterium powder or microorganism in coating releases, in suitable environment
Under the conditions of restoration ecosystem activity, stop being passed through for oxygen, aerobic bacteria by remaining oxygen it is depleted after, carry out anaerobic fermentation mistake
Journey；It is finally completed the different fermentations process of dregs of beans, material is added in a step in this method, avoids miscellaneous bacteria caused by repeatedly feeding dirty
Dye shortens the fermentation time of dregs of beans, reduces production cost.
The dregs of beans of one-step fermentation of the present invention, aspergillus oryzae and aspergillus niger have powerful ectoenzyme system, in dregs of beans of degrading
Macromolecular substances alleviate the stomach and intestine burden of feeding animals, improve the bioavailability of feed, furthermore can also effectively degrade big
Antigen protein in beans reduces its harm to feeding animals；Bacillus cereus, bacillus subtilis and synanthrin gemma cream bar
The gemma of bacterium cooperates, and further anti-nutritional factors is decomposed in fermentation, and increases the content of organic acid in fermentation material.In hops
Contain lupulones, humulone, alkaloid, organic acid and polyphenolic substance；With antibacterial, antitumor you, a variety of function such as anti-oxidant
Energy；Cooperate, complement each other between each component, the dregs of beans after fermentation can significantly improve animal growth, strengthen immunity,
Improve digestion and absorption function.
Specific embodiment
Term as used in the present invention generally has those of ordinary skill in the art usual unless otherwise specified
The meaning of understanding.
Combined with specific embodiments below, and referring to the data further detailed description present invention.Following embodiment only be
It illustrates the present invention, rather than limits the scope of the invention in any way.
Embodiment 1
A kind of microorganism sustained release microbial inoculum of zein coating, is prepared by following methods:
(1) it weighs zein and is mixed with carboxymethyl cellulose by the weight ratio of 5:1, the ethyl alcohol that 5 times of weight 40% are added is molten
In liquid, the glycerol of zein weight 12%, the Tween 80 of zein weight 5% are added；It is uniformly mixed up to coating agent；
(2) microbial bacterial agent is added into coating agent, the additive amount of microbial bacterial agent is the 12% of coating agent weight, closed
Be uniformly mixed rapidly in container, coating liquid made uniformly to be wrapped in the surface of complex micro organism fungicide, be dried to powder to get
The microorganism of zein coating is sustained microbial inoculum.
The microbial bacterial agent presses 1 by the gemma of bacillus cereus, bacillus subtilis and synanthrin lactobacillus:
The mass ratio of 1:1 mixes.
The bacillus cereus (Bacillus cereus) is bacillus cereus J19, deposit number CGMCC
No.10052；
The bacillus subtilis (Bacillus subtilis) is bacillus subtilis ACCC 11025, Chinese agriculture
Microbiological Culture Collection administrative center；
Synanthrin lactobacillus (Sporolactobacillus inulinus) AS1.3758, Bei Na wound connection biology
Science and Technology Ltd..
Embodiment 2
The activation of aspergillus oryzae and aspergillus niger and the preparation of fermenting microbe
Aspergillus oryzae BNCC195382, Bei Na Chuan Lian Bioisystech Co., Ltd；
Aspergillus niger CICC2377, Bei Na Chuan Lian Bioisystech Co., Ltd；
It crushes beans shell and crosses 10 meshes, sterilize through 121 DEG C, 15min；Water content 50~65% is adjusted, will be trained through fermented bean drink inclined-plane
Aspergillus oryzae and aspergillus niger strain after supporting base activation are inoculated in beans shell culture medium, 25-30 DEG C of culture 48 by 1% inoculum concentration respectively
Fermenting microbe, strain content >=10 are made in~60h9cfu·g-1。
Embodiment 3
A kind of one-step fermentation of dregs of beans, steps are as follows:
(1) pretreatment of raw material: dregs of beans crushed 10 meshes, and 121 DEG C of 30min of high pressure steam sterilization are cooling after the completion of sterilizing
To room temperature；
(2) one-step fermentation: the moisture content for adjusting dregs of beans after sterilizing is 75~85%, and 8% aspergillus oryzae is inoculated with into dregs of beans
With the complex micro organism fungicide of the fermenting microbe of 4% aspergillus niger and 6% 1 zein coating of embodiment, after being sufficiently mixed, into
Row fermentation, 28 DEG C~32 DEG C of fermentation temperature of control after keeping moisture content 75~85%, stirring 30~36h of aerobic fermentation, are added
The deionized water of 1 times of amount, stopping oxygen or air are passed through, 48~52h of closed stirring fermentation；
(3) it discharges: the hop residue of material gross weight 1.2% is added, discharges after mixing evenly.
Embodiment 4
A kind of one-step fermentation of dregs of beans, steps are as follows:
(1) pretreatment of raw material: dregs of beans crushed 10 meshes, and 121 DEG C of 30min of high pressure steam sterilization are cooling after the completion of sterilizing
To room temperature；
(2) one-step fermentation: the moisture content for adjusting dregs of beans after sterilizing is 75~85%, and 10% aspergillus oryzae is inoculated with into dregs of beans
With the complex micro organism fungicide of the fermenting microbe of 5% aspergillus niger and 8% 1 zein coating of embodiment, after being sufficiently mixed, into
Row fermentation, 28 DEG C~32 DEG C of fermentation temperature of control after keeping moisture content 75~85%, stirring 30~36h of aerobic fermentation, are added
The deionized water of 1 times of amount, stopping oxygen or air are passed through, 48~52h of closed stirring fermentation；
(3) it discharges: the hop residue of material gross weight 1.0% is added, discharges after mixing evenly.
Embodiment 5
A kind of one-step fermentation of dregs of beans, steps are as follows:
(1) pretreatment of raw material: dregs of beans crushed 10 meshes, and 121 DEG C of 30min of high pressure steam sterilization are cooling after the completion of sterilizing
To room temperature；
(2) one-step fermentation: the moisture content for adjusting dregs of beans after sterilizing is 75~85%, and 7% aspergillus oryzae is inoculated with into dregs of beans
With the complex micro organism fungicide of the fermenting microbe of 3% aspergillus niger and 5% 1 zein coating of embodiment, after being sufficiently mixed, into
Row fermentation, 28 DEG C~32 DEG C of fermentation temperature of control after keeping moisture content 75~85%, stirring 30~36h of aerobic fermentation, are added
The deionized water of 1 times of amount, stopping oxygen or air are passed through, 48~52h of closed stirring fermentation；
(3) it discharges: the hop residue of material gross weight 0.8% is added, discharges after mixing evenly.
Comparative example 1
A kind of one-step fermentation of dregs of beans, steps are as follows:
(1) pretreatment of raw material: dregs of beans crushed 10 meshes, and 121 DEG C of 30min of high pressure steam sterilization are cooling after the completion of sterilizing
To room temperature；
(2) one-step fermentation: the moisture content for adjusting dregs of beans after sterilizing is 75~85%, and 8% aspergillus oryzae is inoculated with into dregs of beans
The compound micro- of the zein coating of carboxymethyl cellulose preparation is not added with the fermenting microbe of 4% aspergillus niger and 6% embodiment 1
Bacteria agent after being sufficiently mixed, ferments, and 28 DEG C~32 DEG C of fermentation temperature of control keeps moisture content 75~85%, stirring
After 30~36h of aerobic fermentation, the deionized water of 1 times of amount is added, stops being passed through for oxygen or air, closed stirring ferments 48~
52h；
(3) it discharges: the hop residue of material gross weight 1.2% is added, discharges after mixing evenly.
Comparative example 2
A kind of one-step fermentation of dregs of beans, steps are as follows:
(1) pretreatment of raw material: dregs of beans crushed 10 meshes, and 121 DEG C of 30min of high pressure steam sterilization are cooling after the completion of sterilizing
To room temperature；
(2) one-step fermentation: the moisture content for adjusting dregs of beans after sterilizing is 75~85%, and 8% aspergillus oryzae is inoculated with into dregs of beans
The compound of the zein coating of synanthrin lactobacillus preparation is not added with the fermenting microbe of 4% aspergillus niger and 6% embodiment 1
Microbial bacterial agent after being sufficiently mixed, ferments, and 28 DEG C~32 DEG C of fermentation temperature of control keeps moisture content 75~85%, stirs
After mixing 30~36h of aerobic fermentation, the deionized water of 1 times of amount is added, stops being passed through for oxygen or air, closed stirring ferments 48~
52h；
(3) it discharges: the hop residue of material gross weight 1.2% is added, discharges after mixing evenly.
Comparative example 3
A kind of one-step fermentation of dregs of beans, steps are as follows:
(1) pretreatment of raw material: dregs of beans crushed 10 meshes, and 121 DEG C of 30min of high pressure steam sterilization are cooling after the completion of sterilizing
To room temperature；
(2) two-step fermentation: the moisture content for adjusting dregs of beans after sterilizing is 75~85%, and 8% aspergillus oryzae is inoculated with into dregs of beans
It after being sufficiently mixed with the fermenting microbe of 4% aspergillus niger, ferments, 28 DEG C~32 DEG C of fermentation temperature of control keeps moisture content
75~85%, after stirring 30~36h of aerobic fermentation, 6% microbial bacterial agent and the deionized water of 1 times of amount is added, stops oxygen
Or air is passed through, closed stirring is fermented 48~52h；
(3) it discharges: the hop residue of material gross weight 1.2% is added, discharges after mixing evenly.
The microbial bacterial agent presses 1:1 by the gemma of bacillus cereus, bacillus subtilis and synanthrin lactobacillus:
1 mass ratio mixes.
One, fermented bean dregs effect test
1.1 test groupings
The fermented bean dregs of embodiment 3~5 and the preparation of 1~3 method of comparative example；
1.2 test method
Selective body heavy phase is close, development is normal, " Australia's lake hybrid sheep " 105 at 3 monthly ages of health, is randomly divided into 7 groups, every group
3 repetitions, 5 sheep of each repetition；Wherein, the basal diet for adding 3~5 fermented bean dregs of embodiment, note are fed respectively for 1~3 group
It is 3~5 groups of embodiment, 4~6 groups feed the basal diet for adding 1~3 fermented bean dregs of comparative example respectively, are denoted as comparative example 1~3
Group, the additive amount of fermented bean dregs are 0.2kg/kg.The composition of basal diet is shown in Table 1.Experimental period is 75 days, wherein preliminary trial period 15
It, the positive examination phase 60 days, normal expelling parasite and epidemic prevention during test, 6 points of every morning and at night 6 points of feedings twice, are freely eaten, drink
Water, records feed intake and weight, each group experimental condition are consistent.
1 basal diet of table and trophic level (dry matter basis)
| It forms (%) | Trophic level2) | ||
| Peanut vine | 30.00 | Digestible energy (MJ/kg) | 11.79 |
| Corn | 24.00 | Digestible energy (MJ/kg) | 9.67 |
| Dregs of beans | 15.00 | Dry matter | 90.84 |
| Wheat-middlings | 14.40 | Crude protein | 14.33 |
| Sheep's hay | 13.00 | Crude fat | 2.10 |
| Wheat bran | 2.00 | Neutral detergent fiber | 32.44 |
| Calcium bicarbonate | 0.44 | Acid detergent fiber | 19.94 |
| Mountain flour | 0.40 | Coarse ash | 9.64 |
| Salt | 0.40 | Calcium | 0.61 |
| Premix1) | 0.36 | Total phosphorus | 0.30 |
| It is total | 100.00 |
1) premix provides for every kilogram of diet: VA 1367IU, VD 194IU, VE 15IU, Fe (FeSO4·7H2O)
74mg, Zn (ZnSO4·7H2O) 46.3mg, Mn (MnSO4·5H2O) 36.5mg, Cu (CuSO4·5H2O) 17.0mg, I (KI)
1.5mg, Co (CoCl2·6H2O) 0.3mg, Se (Na2SeO3)0.3mg。
2) digestible energy, metabolic energy are calculated value, remaining is measured value.
The measurement of 1.3 test indexes and interpretation of result
1.3.1 growth performance
Measurement formal test start, at the end of, the empty body weight of sheep, calculate average daily gain；According to the feed intake of statistics,
Calculate feed-weight ratio.The results are shown in Table 2.
Influence of the fermented bean dregs of 2 the method for the present invention of table preparation to sheep growth performance
| Group | Average daily gain (g) | Average daily gain (g) | Feed-weight ratio | Diarrhea rate (%) |
| Embodiment 3 | 310.83 | 1309.67 | 4.21 | 1.21 |
| Embodiment 4 | 292.83 | 1311.67 | 4.48 | 1.88 |
| Embodiment 5 | 280.83 | 1304.33 | 4.64 | 2.01 |
| Comparative example 1 | 261.17 | 1292.67 | 4.95 | 8.37 |
| Comparative example 2 | 275.67 | 1354.33 | 4.91 | 5.64 |
| Comparative example 3 | 273.33 | 1316.33 | 4.82 | 3.55 |
| Blank control group | 238.67 | 1252.00 | 5.25 | 10.23 |
As shown in Table 2, the fermented bean dregs of 3~5 method of embodiment of the present invention preparation are added in basal diet relative to right
Average daily gain can be dramatically increased for ratio 1~3 and blank control group, reduce feed-weight ratio and diarrhea rate；Wherein embodiment 3
Effect it is best.Fermented bean dregs of the invention can dramatically increase the growth performance of sheep.
1.3.2 enteric microorganism
At the 60th day of test, every group randomly selected 3 sheep, acquired rectum fresh excreta；Each excrement sample takes 1g in sterile
In test tube, sterile saline 9mL is added, after oscillator vibrates, in the dilution for carrying out 10 times of gradients, until dilution reaches
10-7.Suitable dilution is chosen in trial test, carries out plate count.Wherein, lactic acid bacteria uses lactic acid bacteria selective medium
(MRS), Escherichia coli use EMB agar medium, and total aerobic bacteria selects nutrient agar.And calculate every gram of excrement
The viable count (lgCFU/g) of sample.The results are shown in Table 3:
Influence (lgCFU/g) of 3 fermented bean dregs of table to sheep enteric microorganism
| Total aerobic bacteria | Escherichia coli | Lactic acid bacteria | |
| Embodiment 3 | 8.65 | 7.03 | 8.11 |
| Embodiment 4 | 8.79 | 7.14 | 8.06 |
| Embodiment 5 | 8.72 | 7.23 | 7.98 |
| Comparative example 1 | 9.03 | 7.66 | 7.71 |
| Comparative example 2 | 8.91 | 7.43 | 7.78 |
| Comparative example 3 | 8.86 | 7.37 | 7.87 |
| Blank control group | 9.13 | 7.85 | 7.64 |
As shown in Table 3, the fermented bean dregs of the preparation of the embodiment of the present invention 3~5 are added in basal diet relative to comparative example 1
~3 and blank control group the quantity of total aerobic bacteria and Escherichia coli in sheep enteron aisle can be significantly reduced, significantly improve enteron aisle lactic acid
The content of bacterium, this illustrates the adjustable sheep intestinal microflora balance of fermented bean dregs of the invention, significantly improves enteron aisle micro-loop
Border promotes digestion and absorption function, and wherein the effect of embodiment 3 is best.
1.3.3 Biochemical Indices In Serum
On the day before off-test, 3 sheep are randomly selected from each group, on an empty stomach, jugular vein blood collection, blood sample is centrifuged system after standing
Standby serum is for measuring biochemical indicator.
Using the content of ELISA method measurement sheep blood serum Immunoglobulin IgA, IgG, IgM and cytokine TNF-α, by examination
Agent box step is detected.The results are shown in Table 4.
Influence of 4 fermented bean dregs of table to sheep blood serum biochemical indicator
| IgA(mg/L) | IgG(mg/L) | IgM(mg/L) | TNF-α(mg/L) | |
| Embodiment 3 | 2.66 | 38.55 | 2.48 | 483.45 |
| Embodiment 4 | 2.62 | 37.82 | 2.41 | 475.63 |
| Embodiment 5 | 2.57 | 37.61 | 2.35 | 470.18 |
| Comparative example 1 | 2.03 | 30.18 | 1.86 | 395.74 |
| Comparative example 2 | 2.24 | 32.36 | 2.03 | 421.86 |
| Comparative example 3 | 2.51 | 35.15 | 2.18 | 458.37 |
| Blank control group | 1.75 | 26.47 | 1.54 | 376.71 |
As shown in Table 4, the fermented bean dregs of the preparation of the embodiment of the present invention 3~5 are added in basal diet relative to comparative example 1
~3 and blank control group can significantly improve the content of sheep blood serum Immunoglobulin IgA, IgG, IgM and cytokine TNF-α,
It can be seen that fermented bean dregs of the invention can significantly increase the immunity of sheep, wherein the effect of embodiment 3 is best.
The above described is only a preferred embodiment of the present invention, being not that the invention has other forms of limitations, appoint
What those skilled in the art changed or be modified as possibly also with the technology contents of the disclosure above equivalent variations etc.
Imitate embodiment.But without departing from the technical solutions of the present invention, according to the technical essence of the invention to above embodiments institute
Any simple modification, equivalent variations and the remodeling made, still fall within the protection scope of technical solution of the present invention.
Claims (10)
1. a kind of microorganism of zein coating is sustained microbial inoculum, it is characterised in that: be prepared by following methods:
(1) it weighs zein and is mixed with carboxymethyl cellulose by the weight ratio of 5:1, is added in the ethanol solution of 5 times of weight 40%,
Add the glycerol of zein weight 12%, the Tween 80 of zein weight 5%；It is uniformly mixed up to coating agent；
(2) microbial bacterial agent is added into coating agent, the additive amount of microbial bacterial agent is the 12% of coating agent weight, closed container
In be uniformly mixed rapidly, so that coating liquid is uniformly wrapped in the surface of complex micro organism fungicide, be dried to powder to get corn
The microorganism of protein coating is sustained microbial inoculum.
2. the microorganism of zein coating according to claim 1 is sustained microbial inoculum, it is characterised in that: the microbial bacteria
Agent is the active hypopus of microbial activity bacterium powder or microorganism.
3. the microorganism of zein coating according to claim 1 or 2 is sustained microbial inoculum, it is characterised in that: micro- life
Object microbial inoculum by bacillus cereus, bacillus subtilis and synanthrin lactobacillus gemma by 1:1:1 mass ratio mixing and
At.
4. microorganism sustained release microbial inoculum the answering in animal and fowl fodder fermentation of the described in any item zein coatings of claims 1 to 3
With.
5. a kind of one-step fermentation of dregs of beans, it is characterised in that: steps are as follows:
(1) pretreatment of raw material: dregs of beans crushed 10 meshes, and 121 DEG C of 30min of high pressure steam sterilization after the completion of sterilizing, are cooled to room
Temperature；
(2) one-step fermentation: the moisture content for adjusting dregs of beans after sterilizing is 75~85%, and aspergillus oryzae and aspergillus niger are inoculated with into dregs of beans
Fermenting microbe and claim 3 zein coating complex micro organism fungicide, after being sufficiently mixed, ferment, control
28 DEG C~32 DEG C of fermentation temperature, keep moisture content 75~85%, stirring 30~36h of aerobic fermentation after, be added 1 times amount go from
Sub- water, stopping oxygen or air are passed through, 48~52h of closed stirring fermentation；
(3) it discharges: the hop residue of material gross weight 0.8~1.2% is added, discharges after mixing evenly.
6. the one-step fermentation of dregs of beans according to claim 5, it is characterised in that:
The inoculum concentration of the aspergillus oryzae fermenting microbe is 7~10%, and the inoculum concentration of the fermentation of Aspergillus niger strain is 3~5%；
The additive amount of the complex micro organism fungicide of the zein coating is the 5~8% of dregs of beans weight.
7. the one-step fermentation of dregs of beans according to claim 5, it is characterised in that: the zymophyte of the aspergillus oryzae and aspergillus niger
Kind is prepared by following methods:
It crushes beans shell and crosses 10 meshes, sterilize through 121 DEG C, 15min；Water content 50~65% is adjusted, it will be through fermented bean drink slant medium
Aspergillus oryzae and aspergillus niger strain after activation are inoculated in beans shell culture medium by 1% inoculum concentration respectively, and 25-30 DEG C of culture 48~
Fermenting microbe, strain content >=10 are made in 60h9cfu·g-1Beans shell culture medium.
8. the dregs of beans of any one of claim 5~7 the method fermentation.
9. fermented bean dregs according to any one of claims 8 improve animal growth, strengthen immunity, improvement digestion and absorption function in preparation
Application in energy feed.
10. a kind of improve animal growth, strengthen immunity, the feed for improving digestion and absorption function, it is characterised in that: raising
Fermented bean dregs according to any one of claims 8 are added in material, additive amount is 0.1~0.25kg/kg feed.
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