CN108330088A - Solid microbial and preparation method thereof - Google Patents
Solid microbial and preparation method thereof Download PDFInfo
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- CN108330088A CN108330088A CN201810167113.9A CN201810167113A CN108330088A CN 108330088 A CN108330088 A CN 108330088A CN 201810167113 A CN201810167113 A CN 201810167113A CN 108330088 A CN108330088 A CN 108330088A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
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Abstract
The invention belongs to agricultural biochemical arts, and in particular to solid microbial and preparation method thereof.The solid microbial includes the raw material of following parts by weight：100 parts of microbial fermentation solution, 150~300 parts of solid absorbent, 40~60 parts of coating agent；The coating agent is polyethylene glycol and polypropylene glycol according to weight ratio 60:40~80:20 mixture or polyethyleneglycol content is poly- (ethylene glycol propylene glycol) copolymer of 60~80wt.%；The coating agent, which is dissolved in glycol ether solvents, carries out film.The microbial bacterial agent provides in solid form, is readily transported, stores and uses, while having desired sustained release performance.The invention further relates to the preparation methods of the solid microbial.
Description
Technical field
The invention belongs to agricultural biochemical arts, and in particular to solid microbial and preparation method thereof.
Background technology
Microbial bacterial agent refers to say the beneficial microbe mass production breeding with specific function with thinking after, being made containing work
The product of predetermined substance in thalline, also commonly referred to as microorganism formulation or microbial inoculant (《Environmental pollution microbiology》, often
Learn elegant), not only in such as pollutant process field, microbial bacterial agent is same in many agricultural uses such as getting fat volume increase, sterilization sterilizings
Sample have extensively using (《Common Fertilizer application Manual Amendment version》, Tu Shihua).
In the prior art, strain selection, compounding and fermenting and producing etc. are concentrated on for the application of microbial bacterial agent
(CN107058167A, CN105400720A, CN107118998A), product generally contains fermented liquid, thus there are products
Storage, transport difficult, culture presevation requires high drawback.In this regard, being further disclosed in the prior art through various solids
Material load containing fermented liquid, to be prepared solid microbial technical solution (CN101861880A,
CN102174443A、CN104211449A).But above-mentioned technical proposal is only that liquid formulation load is merely obtained solid-state
Preparation, this preparation especially agrochemical field, once strain can be caused to be flowed with rain drop erosion rapidly in input soil
It loses, without any slow release effect.The microorganism particle microbial inoculum (CN102925426A) of coating, but its are also disclosed in the prior art
Purpose is to prevent sticking together between particle, and it uses the urea of molten state, waterglass etc. to be used as coated fertilizer, visitor
There is a problem of microorganism death in sight, and this coated fertilizer still cannot achieve the sustained release of microbial bacterial agent.
Invention content
To solve problems of the prior art, one aspect of the invention is related to solid microbial, micro- life
Object microbial inoculum provides in solid form, is readily transported, stores and uses, while having desired sustained release performance.
Specifically, the solid microbial includes the raw material of following parts by weight：
100 parts of microbial fermentation solution
150~300 parts of solid absorbent
40~60 parts of coating agent；
The coating agent is polyethylene glycol and polypropylene glycol according to weight ratio 60:40~80:20 mixture or poly- second
Glycol content is poly- (ethylene glycol and 1,2-propylene glycol) copolymer of 60~80wt.%；
The coating agent, which is dissolved in glycol ether solvents, carries out film.
The preparation of the microbial fermentation solution of the present invention and the microbial bacterial agent that particle is obtained by solid absorbent,
Method disclosed in the prior art can be used to realize.The type of microorganism is not particularly limited, can be according to for solid microbe
The desired effect of microbial inoculum is selected；Especially for the application of agriculture chemistry getting fat synergy, preferably be selected from bacillus subtilis,
Bacillus megaterium, bacillusmusilaginosiengineering, bacillus amyloliquefaciens, bacillus licheniformis, colloid bacillus cereus, phosphorus-solubilizing bacteria are huge
The combination of one or more of Bacterium anthracoides, saccharomycete.The solid absorbent can specifically use diatomite, height
The combination of one or more of ridge soil, vermiculite power, medical stone powder, crushed crude pearlite, zeolite powder, further preferred solid are inhaled
The attached dose of grain size with 100~200 μ ms, in order to the absorption and dispersion to microorganism.
The present invention has by the improvement to coating agent and its coating process to be provided for granular microbial bacterial agent
The coating of slow releasing function, and abandoned the work for needing to introduce water-insoluble coating in the prior art or needing high temperature film
Skill farthest reduces the inactivation of microorganism.Specifically, the present invention uses the combination of polyethylene glycol and polypropylene glycol
Object or poly- (ethylene glycol and 1,2-propylene glycol) copolymer are as coating agent, to realize balance of the coated fertilizer on parent, water repellency, from
And solid microbial is assigned with adjustable sustained release performance.Preferably, the polyethylene glycol has the number of 1000-1500
Average molecular weight, molecular weight is too low, then the mechanical property of coating is insufficient and water-soluble too strong；Its molecular weight is excessive, then influences to wrap
The release of the water-soluble and microbial cells of film.Matching, the further preferred polypropylene glycol is equal with 600~1000 number
Molecular weight.In addition, for same consideration, poly- (ethylene glycol and 1,2-propylene glycol) is preferably with 1200~2000 equal molecule of number
Amount.
The type of the glycol ether solvents is not particularly limited, as long as it effectively can dissolve coated fertilizer and unlikely
In making, the solid absorbent of loading microorganisms microbial inoculum bursts apart or loaded article falls off.Ethylene glycol, two can specifically be enumerated
The monoether and/or diether of glycol, and/or triethylene glycol；The ether has 1~5 carbon atom；It is examined from the angle for reducing solvent toxicity
Consider, preferably diethyleneglycol diether；From the point of view of improving dissolubility, further preferred diethylene glycol dimethyl ether.Solvent should not use
Water or other intensive polar solvents：Shipwreck is inclined to the stripping for leading to adsorb thalline effectively to be dissolved to polypropylene glycol or its segment
It falls；Other intensive polar solvents such as chloroform, ethyl alcohol etc. have bio-toxicity, easily cause the inactivation of microorganism.
Another aspect of the invention is related to the preparation method of solid microbial, includes the following steps：
(1) microbial fermentation solution and solid absorbent are mixed in proportion, obtains the solid absorbent of loading microorganisms；
(2) coating agent is dissolved in glycol ether solvents, obtains coating agent solution；
(3) solid absorbent of the loading microorganisms of step (1) is put into the coating agent solution of step (2), after stirring
Spray drying.
The coating agent solution preferably concentration with 25~45wt.%.Then coating agent is possible to dissolving not to excessive concentration
Fully and cause particle difficulties in dispersion；Concentration is too low, cannot achieve effective film, and remove excess of solvent also arrive energy consumption,
The waste of raw material.
The coating process of the present invention should not use traditional fountain coating, imperfect and cause it slow to prevent particle envelope
Releasing effect can not play.
The temperature of the spray drying is controlled at 60~80 DEG C, to prevent the excessively high injury to microorganism of temperature.
The beneficial effects of the present invention are：
(1) a kind of solid microbial is provided, the microbial inoculum has good flowing dispersibility, and in chemurgy
Etc. the desired slow release effect in fields.
(2) preparation method for providing the solid microbial does not specially require strain, can widen the micro- life of solid
The application range of object microbial inoculum；The method is simple and practicable, and low energy consumption, effect is good.
Specific implementation mode
Below in conjunction with the embodiment of the present invention, technical scheme of the present invention is clearly and completely described.Based on this
Embodiment in invention, the every other reality that those of ordinary skill in the art are obtained without creative efforts
Example is applied, shall fall within the protection scope of the present invention.
Embodiment 1
28gPEG (Mn=1000), 12gPPG (Mn=700) are added in 100g diethylene glycol dimethyl ethers, is sufficiently stirred and is allowed to
Dissolving, obtains coating agent solution.
Be added into 100g bacillus subtilises (Bacillus subtilis) zymotic fluid 200g diatomite (d=150~
180 μm), after being sufficiently stirred, adsorbing, obtain the solid absorbent of muddy loading microorganisms.
The solid absorbent of loading microorganisms is added in aforementioned coating agent solution, is sufficiently stirred after keeping film abundant,
It is spray-dried at 65 DEG C, obtains solid microbial.
Embodiment 2
By poly- (ethylene glycol and 1,2-propylene glycol-ethylene glycol) block copolymers of 60g (ethylene glycol segment accounts for 50wt.%, Mn=1800)
It puts into 200g diethylene glycol dimethyl ethers, is sufficiently stirred and is allowed to dissolve, obtain coating agent solution.
By 50g bacillus megateriums (Bacillusmegaterium) zymotic fluid, 30g bacillusmusilaginosiengineerings
(Bacillus mucilagimosuskrassilm) zymotic fluid and 20g bacillus amyloliquefaciens
(bacillusamgloliquefaciens) zymotic fluid and 300g solid absorbent (diatomite：Kaolin：Zeolite powder=10:
10：80, weight ratio) mixing obtains the solid absorbent of muddy loading microorganisms after being sufficiently stirred, adsorbing.
The solid absorbent of loading microorganisms is added in aforementioned coating agent solution, is sufficiently stirred after keeping film abundant,
It is spray-dried at 75 DEG C, obtains solid microbial.
Comparative example 1
On the basis of embodiment 1, diethylene glycol dimethyl ether is substituted using ethyl alcohol, remaining is identical.
Comparative example 2
On the basis of embodiment 1, diethylene glycol dimethyl ether is replaced using deionized water, remaining is identical.
Comparative example 3
On the basis of embodiment 2, diethylene glycol dimethyl ether is replaced using deionized water, remaining is identical.
According to《GB 20287-2006 agricultural microbial agents》, the living bacteria count (hundred million/g) of before-mentioned products is surveyed
It is fixed, as a result it is listed in table 1." after preparation " refers to that sample is the sample after the completion of being prepared according to aforementioned each example；" after sustained release " refers to
It is that the sample after the completion of aforementioned each example preparation is positioned in non-woven bag, according to 10g/1m2The amount in soil is launched, and is returned after 30 days
Receive the testing result of sample.
Table 1
| Sample | Embodiment 1 | Embodiment 2 | Comparative example 1 | Comparative example 2 | Comparative example 3 |
| After preparation | 3.5 | 3.7 | 0.3 | 2.2 | 2.3 |
| After sustained release | 1.6 | 1.8 | 0.1 | 0.3 | 1.2 |
Comparative example 1, as coating agent solvent, leads to microorganism mortality using the ethyl alcohol with bio-toxicity；Comparative example
2 use water as solvent, and PPG, which can be observed, in preparing coating agent solution processes effectively to dissolve, and lead to the slow of product
The property released, which reduces, microorganism is rapid during sustained release is lost in；The use water of comparative example 2 and comparative example 3 is also another as solvent
A problem undesirably elutes microorganism during coating from solid absorbent, lead to effective viable bacteria of its product
Number is relatively low.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, and should all contain
Lid is within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (10)
1. solid microbial, which is characterized in that include the raw material of following parts by weight：
100 parts of microbial fermentation solution
150~300 parts of solid absorbent
40~60 parts of coating agent；
The coating agent is polyethylene glycol and polypropylene glycol according to weight ratio 60:40~80:20 mixture or polyethylene glycol
Content is poly- (ethylene glycol and 1,2-propylene glycol) copolymer of 60~80wt.%；
The coating agent, which is dissolved in glycol ether solvents, carries out film.
2. solid microbial according to claim 1, which is characterized in that the microorganism of the microbial fermentation solution,
Selected from bacillus subtilis, bacillus megaterium, bacillusmusilaginosiengineering, bacillus amyloliquefaciens, bacillus licheniformis, colloid
The combination of one or more of bacillus, phosphorus-solubilizing bacteria bacillus megaterium, saccharomycete.
3. solid microbial according to claim 1, which is characterized in that the grain size of the solid absorbent is 100
~200 μm.
4. solid microbial according to claim 3, which is characterized in that the solid absorbent be selected from diatomite,
The combination of one or more of kaolin, vermiculite power, medical stone powder, crushed crude pearlite, zeolite powder
5. solid microbial according to claim 1, which is characterized in that the polyethylene glycol has 1000-1500
Number-average molecular weight, the polypropylene glycol have 600~1000 number-average molecular weight.
6. solid microbial according to claim 1, which is characterized in that poly- (ethylene glycol and 1,2-propylene glycol) has
1200~2000 number-average molecular weight.
7. solid microbial according to claim 1, which is characterized in that the glycol ether solvents are selected from second two
The monoether and/or diether of alcohol, diethylene glycol (DEG), and/or triethylene glycol.
8. solid microbial according to claim 1, which is characterized in that the glycol ether solvents are selected from diethylene glycol (DEG)
Diether, preferably diethylene glycol dimethyl ether.
9. according to the preparation method of claim 1~8 any one of them solid microbial, which is characterized in that including with
Lower step：
(1) microbial fermentation solution and solid absorbent are mixed in proportion, obtains the solid absorbent of loading microorganisms；
(2) coating agent is dissolved in glycol ether solvents, obtains coating agent solution；
(3) it by the coating agent solution of the solid absorbent of the loading microorganisms of step (1) input step (2), sprays after stirring
It is dry.
10. preparation method according to claim 9, which is characterized in that the coating agent solution has 25~45wt.%'s
Concentration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810167113.9A CN108330088A (en) | 2018-02-28 | 2018-02-28 | Solid microbial and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810167113.9A CN108330088A (en) | 2018-02-28 | 2018-02-28 | Solid microbial and preparation method thereof |
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| Publication Number | Publication Date |
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| CN108330088A true CN108330088A (en) | 2018-07-27 |
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| CN201810167113.9A Pending CN108330088A (en) | 2018-02-28 | 2018-02-28 | Solid microbial and preparation method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109258930A (en) * | 2018-11-01 | 2019-01-25 | 青岛农业大学 | A kind of one-step fermentation of dregs of beans, the fermented bean dregs of this method preparation and application |
| CN110468063A (en) * | 2019-04-17 | 2019-11-19 | 浙江亲水园生物科技有限公司 | A kind of microbial solid preparation, preparation method and application |
| CN111436349A (en) * | 2020-03-13 | 2020-07-24 | 河海大学 | Seedling raising substrate for cultivating salt-resistant plant seedlings and preparation method and application thereof |
| CN118084569A (en) * | 2024-03-01 | 2024-05-28 | 吉林农业大学 | Rice slow-release fertilizer and application thereof |
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| CN102925426A (en) * | 2012-11-23 | 2013-02-13 | 北京世纪阿姆斯生物技术股份有限公司 | Coated granular microbial agent and production method thereof |
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| CN1910217A (en) * | 2004-01-15 | 2007-02-07 | 伊诺科雷技术有限公司 | Biogradable multi-block co-polymers |
| CN102925426A (en) * | 2012-11-23 | 2013-02-13 | 北京世纪阿姆斯生物技术股份有限公司 | Coated granular microbial agent and production method thereof |
| WO2018009549A1 (en) * | 2016-07-06 | 2018-01-11 | Crop Enhancement, Inc. | Nontoxic coating concentrates for agricultural uses |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109258930A (en) * | 2018-11-01 | 2019-01-25 | 青岛农业大学 | A kind of one-step fermentation of dregs of beans, the fermented bean dregs of this method preparation and application |
| CN110468063A (en) * | 2019-04-17 | 2019-11-19 | 浙江亲水园生物科技有限公司 | A kind of microbial solid preparation, preparation method and application |
| CN111436349A (en) * | 2020-03-13 | 2020-07-24 | 河海大学 | Seedling raising substrate for cultivating salt-resistant plant seedlings and preparation method and application thereof |
| CN118084569A (en) * | 2024-03-01 | 2024-05-28 | 吉林农业大学 | Rice slow-release fertilizer and application thereof |
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