CN109248103A - A kind of dental care products of anti-helicobacter pylori - Google Patents
A kind of dental care products of anti-helicobacter pylori Download PDFInfo
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- CN109248103A CN109248103A CN201811278710.5A CN201811278710A CN109248103A CN 109248103 A CN109248103 A CN 109248103A CN 201811278710 A CN201811278710 A CN 201811278710A CN 109248103 A CN109248103 A CN 109248103A
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- Prior art keywords
- helicobacter pylori
- oleanolic acid
- care products
- dental care
- acid
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/63—Steroids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/347—Phenols
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
Abstract
The embodiment of the invention discloses a kind of dental care products of anti-helicobacter pylori, the dental care products contain oleanolic acid, active constituent of the oleanolic acid as anti-helicobacter pylori.The embodiment of the present invention has the advantages that the helicobacter pylori that can be killed or inhibit in oral cavity, helicobacter pylori so as to avoid (such as teeth space, calculus dentalis etc.) in oral cavity constantly enters alimentary canal with saliva, and leads to the relevant disease of digestive tract recurrent exerbation of helicobacter pylori；To overcome conventional therapy helicobacter pylori infections only to stomach and intestine medication, this helicobacter pylori of oral cavity aggregation ground is had ignored, so that the always continuous repeated infection of disease of digestive tract caused by helicobacter pylori, it is difficult to the problem of eradicating.
Description
Technical field
The present invention relates to oral cleaning technical fields, and in particular to a kind of dental care products of anti-helicobacter pylori.
Background technique
Helicobacter pylori is generally acknowledged pathogenic bacteria, is classified as the level-one carcinogenic substance (superlative degree, to the mankind by the World Health Organization
It is carcinogenic for determination).It is closely related with stomach trouble (gastritis, ulcer, stomach tumor, gastric cancer).After infecting helicobacter pylori, gastric cancer is obtained
Sick risk increases more times.
How helicobacter pylori is effectively treated or prevented, is of great significance to prevention and control gastric cancer.
Currently, the prevention and treatment of helicobacter pylori is a global problem, authoritative recommended therapy (antibiotic+bismuth preparation+suppression
Sour agent) also only have 70% or so eradication rate, and as Hp Drug Resistance rate rises year by year, helicobacter pylori eradication
Rate declines year by year；And antibiotic+bismuth preparation+acid inhibitor therapy side effect is big.
In view of global almost half population infection helicobacter pylori, the soaring of Hp Drug Resistance, helicobacter pylorus
The reality of the decline of bacterium eradication rate.Each state is all paying attention to and is researching and developing the drug of prevention and control helicobacter pylori (Main way is still
It is antibiotic direction).
Summary of the invention
Helicobacter pylori is present in digestion, is mainly gathered in oral cavity and stomachus pyloricus position, and conventional therapy is ignored
Oral cavity, the helicobacter pylori in oral cavity, which follows saliva etc. constantly to enter in stomach, leads to stomach trouble recurrent exerbation, it is difficult to eradicate.
A kind of dental care products for being designed to provide anti-helicobacter pylori of the embodiment of the present invention, to for kill
Or the helicobacter pylori in inhibition oral cavity, to overcome the problems, such as that stomach trouble caused by helicobacter pylori is difficult to eradicate.
To achieve the above object, first aspect of the embodiment of the present invention provides a kind of oral cleaning of anti-helicobacter pylori
Articles, the dental care products contain oleanolic acid, active constituent of the oleanolic acid as anti-helicobacter pylori.
In one possible implementation, in the dental care products, content >=16mg/ of oleanolic acid
100ml。
In one possible implementation, the oleanolic acid in the dental care products is that the first natural plants mention
Take object；The raw material for preparing of first natural plant extracts includes following any one or more:
Olive leaf, arched bottom, the fruit of glossy privet, mussot swertia herb；
First natural extract is prepared by the first oleanolic acid extracting method or the second oleanolic acid extracting method
It obtains:
First oleanolic acid extracting method includes: by one of olive leaf, arched bottom, the fruit of glossy privet, mussot swertia herb
Or it is a variety of broken, 20 meshes are crossed, medicinal powder is obtained；Medicinal powder is added in 70% (v/v) ethyl alcohol of 5 times of weight, refluxing extraction is extremely
3 times few, each used time 2.5-3.5 hours, alcohol extract merged；Distillation removal ethyl alcohol, is then added the concentrated sulfuric acid, until sulfuric acid is dense
Degree is that 15% (w/v) is stirred continuously in concentrated sulfuric acid adition process；Reflux 2 hours, is cooled to 5-10 DEG C, uses filter cake mistake
Filter, obtains crude extract；Crude extract is added in 85% (v/v) ethyl alcohol of 8 times of weight, and the activity of 0.04 times of weight is added
Charcoal flows back 40 minutes, and filtering obtains filtrate；The pH modulation 3-4 of filtrate is used into steaming using cake filtration using dilute hydrochloric acid
Distilled water filter wash cake removes oleanolic acid after filtration cakes torrefaction from filter cake；
Second oleanolic acid extracting method is the following steps are included: by olive leaf, arched bottom, the fruit of glossy privet, mussot swertia herb
One of or it is a variety of broken, cross 20 meshes, obtain medicinal powder；Medicinal powder is added in the water of 20 times of weight, decoction 3 times, every time
1 hour, merge decocting liquid；Decocting liquid is concentrated to dilute cream shape, the concentrated sulfuric acid is added, until the concentration of sulfuric acid is 20% (w/v), dense
In sulfuric acid adition process, it is stirred continuously；2-5 times of water dilution is added, uses cake filtration；Filter cake is washed, then uses 3%
(w/v) sodium hydroxide solution boils filter cake 2 times, and the liquid after boiling every time filters filter cake again, reuses boiling boiling filter cake, boils
The liquid of boiling filters filter cake again；Obtain crude extract；Crude extract is added in 85% (v/v) ethyl alcohol of 8 times of weight, and is added
The active carbon of 0.04 times of weight flows back 40 minutes, and filtering obtains filtrate；The pH of filtrate is modulated into 3-4 using dilute hydrochloric acid, is used
After filtration cakes torrefaction, oleanolic acid is removed from filter cake using distilled water filter wash cake for cake filtration.
In a possible implementation, the dental care products are to include but is not limited to any of the following:
Toothpaste, tooth powder, mouthwash, chewing gum, chewable tablets.
Second aspect of the embodiment of the present invention provides a kind of dental care products of anti-helicobacter pylori, and the oral cavity is clear
Clean articles contain oleanolic acid and Paeonol, and oleanolic acid and Paeonol combine the active constituent as anti-helicobacter pylori.
In one possible implementation, the Paeonol in the dental care products is the second natural plants
Extract；
The raw material for preparing of second natural plant extracts includes:
Moutan root bark and/or paniculate swallowwort；
Second natural extract is prepared by the following method to obtain:
Moutan root bark and/or paniculate swallowwort are crushed, 20 meshes is crossed, obtains medicinal powder；
Medicinal powder is placed in distillation extractor, 15 times of the water that quality is medicinal powder quality is added, decocts and extracts 10 hours,
Distillate is collected to filter using qualitative filter paper using vortice vortex oscillation 2 hours, and obtained filtrate；Wherein, in the vibration that is vortexed
When swinging and filtering, temperature is maintained at 60 DEG C or more；
Filtrate freeze-drying, obtains Paeonol.
In one possible implementation, in the dental care products, the content of oleanolic acid, Paeonol
Content and >=16mg/100ml.
In one possible implementation, in the dental care products, the weight ratio of oleanolic acid and Paeonol
For 1:99-99:1.
In one possible implementation, in the dental care products, the weight ratio of oleanolic acid and Paeonol
For 50:50.
In a possible implementation, the dental care products are including but not limited to following any:
Toothpaste, tooth powder, mouthwash, chewing gum, chewable tablets.
The third aspect of the embodiment of the present invention provides oleanolic acid in the dental care products for preparing anti-helicobacter pylori
Purposes.
In a possible implementation, the dental care products are including but not limited to following any:
Toothpaste, tooth powder, mouthwash, chewing gum, chewable tablets.
Fourth aspect of the embodiment of the present invention provides oleanolic acid and Paeonol is combined in the mouth for preparing anti-helicobacter pylori
Purposes in chamber cleaning supplies.
In a possible implementation, the dental care products are comprising following any:
Toothpaste, tooth powder, mouthwash, chewing gum, chewable tablets.
The embodiment of the present invention has the advantages that the helicobacter pylori that can be killed or inhibit in oral cavity, so as to avoid
The helicobacter pylori of (such as teeth space, calculus dentalis etc.) constantly enters alimentary canal with saliva in oral cavity, and leads to helicobacter pylori
Related disease of digestive tract recurrent exerbation；To overcome conventional therapy helicobacter pylori infections only to stomach and intestine medication, mouth is had ignored
This helicobacter pylori of chamber aggregation ground, so that the always continuous repeated infection of disease of digestive tract caused by helicobacter pylori, it is difficult to
The problem of radical cure.
Specific embodiment
Embodiments of the present invention are illustrated by particular specific embodiment below, those skilled in the art can be by this theory
Content disclosed by bright book is understood other advantages and efficacy of the present invention easily.
Oleanolic acid (oleanolic acid) can also become oleanolic acid hydrate, oleanolic acid, caryophyllin, soil
Radix Angelicae Sinensis acid hydrate；Its molecular formula is C30H48O3, molecular structural formula is as follows:
Paeonol (Paeonol) is also referred to as peonol, paeonol, the entitled 2'-Hydroxy-4'- of chemistry
Methoxyacetopheone, Chinese chemical name are 2'- hydroxyl -4 '-methoxyacetophenone, chemical formula C9H10O3, molecule knot
Structure formula is as follows:
Dental care products provided in an embodiment of the present invention can be toothpaste, or mouthwash, or tooth
Powder, or chewing gum, or chewable tablets.
Next, in a particular embodiment, being carried out by taking toothpaste as an example to dental care products provided in an embodiment of the present invention
It illustrates.
Embodiment 1
A kind of toothpaste of anti-helicobacter pylori is present embodiments provided, the toothpaste contains oleanolic acid, the olive
Active constituent of the acid as anti-helicobacter pylori.
In the present embodiment, oleanolic acid is commercially available (the upper graceful Biotechnology Co., Ltd of Hypon, article No.: D0117).
In toothpaste, the content of oleanolic acid is 16mg/100ml, i.e., contains 16mg oleanolic acid in every 100ml toothpaste.
The other components of toothpaste are referred to formulation of tooth-paste realization in the prior art, and details are not described herein again.
The embodiment of the present invention has the advantages that the helicobacter pylori that can be killed or inhibit in oral cavity, so as to avoid
The helicobacter pylori of (such as teeth space, calculus dentalis etc.) constantly enters alimentary canal with saliva in oral cavity, and leads to helicobacter pylori
Relevant disease of digestive tract recurrent exerbation；To overcome conventional therapy helicobacter pylori infections only to stomach and intestine medication, have ignored
This helicobacter pylori of oral cavity aggregation ground, so that the always continuous repeated infection of disease of digestive tract caused by helicobacter pylori, difficult
The problem of to eradicate.
Embodiment 2
A kind of toothpaste of anti-helicobacter pylori is present embodiments provided, the toothpaste contains oleanolic acid, and oleanolic acid is made
For the active constituent of anti-helicobacter pylori.
In toothpaste, the content of oleanolic acid is 16mg/100ml, i.e., contains 16mg oleanolic acid in every 100ml toothpaste.
Oleanolic acid is the natural plant extracts extracted from arched bottom.Extracting method is as follows:
Arched bottom is crushed, 20 meshes is crossed, obtains medicinal powder；Medicinal powder is added in 70% (v/v) ethyl alcohol of 5 times of weight,
Refluxing extraction at least 3 times, each used time 2.5-3.5 hours, alcohol extract merged；Distillation removal ethyl alcohol, is then added the concentrated sulfuric acid,
Concentration to sulfuric acid is that 15% (w/v) is stirred continuously in concentrated sulfuric acid adition process；Reflux 2 hours, is cooled to 5-10 DEG C,
Using cake filtration, crude extract is obtained；Crude extract is added in 85% (v/v) ethyl alcohol of 8 times of weight, and is added 0.04 times
The active carbon of weight flows back 40 minutes, and filtering obtains filtrate；The pH of filtrate is modulated into 3-4 using dilute hydrochloric acid, uses filter cake mistake
Filter, using distilled water filter wash cake, after filtration cakes torrefaction, oleanolic acid is removed from filter cake, obtains oleanolic acid.
The other components of toothpaste are referred to formulation of tooth-paste realization in the prior art, and details are not described herein again.
The embodiment of the present invention has the advantages that the helicobacter pylori that can be killed or inhibit in oral cavity, so as to avoid
The helicobacter pylori of (such as teeth space, calculus dentalis etc.) constantly enters alimentary canal with saliva in oral cavity, and leads to helicobacter pylori
Relevant disease of digestive tract recurrent exerbation；To overcome conventional therapy helicobacter pylori infections only to stomach and intestine medication, have ignored
This helicobacter pylori of oral cavity aggregation ground, so that the always continuous repeated infection of disease of digestive tract caused by helicobacter pylori, difficult
The problem of to eradicate.
Embodiment 3
A kind of toothpaste of anti-helicobacter pylori is present embodiments provided, the toothpaste contains oleanolic acid, and oleanolic acid is made
For the active constituent of anti-helicobacter pylori.
In toothpaste, the content of oleanolic acid is 16mg/100ml, i.e., contains 16mg oleanolic acid in every 100ml toothpaste.
Oleanolic acid is to extract obtained natural plant extracts from the fruit of glossy privet (fruit for being specifically as follows the fruit of glossy privet).
Extracting method is as follows:
The fruit of glossy privet is crushed, 20 meshes is crossed, obtains medicinal powder；Medicinal powder is added in the water of 20 times of weight, decoction 3 times, often
Secondary 1 hour, merge decocting liquid；Decocting liquid is concentrated to dilute cream shape, the concentrated sulfuric acid is added, until the concentration of sulfuric acid is 20% (w/v),
In concentrated sulfuric acid adition process, it is stirred continuously；2-5 times of water dilution is added, uses cake filtration；Filter cake is washed, then uses 3%
(w/v) sodium hydroxide solution boils filter cake 2 times, and the liquid after boiling every time filters filter cake again, reuses boiling boiling filter cake, boils
The liquid of boiling filters filter cake again；Obtain crude extract；Crude extract is added in 85% (v/v) ethyl alcohol of 8 times of weight, and is added
The active carbon of 0.04 times of weight flows back 40 minutes, and filtering obtains filtrate；The pH of filtrate is modulated into 3-4 using dilute hydrochloric acid, is used
After filtration cakes torrefaction, oleanolic acid is removed from filter cake using distilled water filter wash cake for cake filtration.
The other components of toothpaste are referred to formulation of tooth-paste realization in the prior art, and details are not described herein again.
The embodiment of the present invention has the advantages that the helicobacter pylori that can be killed or inhibit in oral cavity, so as to avoid
The helicobacter pylori of (such as teeth space, calculus dentalis etc.) constantly enters alimentary canal with saliva in oral cavity, and leads to helicobacter pylori
Relevant disease of digestive tract recurrent exerbation；To overcome conventional therapy helicobacter pylori infections only to stomach and intestine medication, have ignored
This helicobacter pylori of oral cavity aggregation ground, so that the always continuous repeated infection of disease of digestive tract caused by helicobacter pylori, difficult
The problem of to eradicate.
Embodiment 4
A kind of toothpaste of anti-helicobacter pylori is present embodiments provided, the toothpaste contains oleanolic acid, and oleanolic acid is made
For the active constituent of anti-helicobacter pylori.
In toothpaste, the content of oleanolic acid is 16mg/100ml, i.e., contains 16mg oleanolic acid in every 100ml toothpaste.
Oleanolic acid is to extract obtained natural plants from oleanolic acid leaf (leaf that oleanolic acid leaf is oleanolic acid)
Extract.Extracting method is as follows:
Oleanolic acid leaf is crushed, 20 meshes is crossed, obtains medicinal powder；Medicinal powder is added to 70% (v/v) second of 5 times of weight
In alcohol, refluxing extraction at least 3 times, each used time 2.5-3.5 hours, alcohol extract merged；Distillation removal ethyl alcohol, is then added dense
Sulfuric acid, until the concentration of sulfuric acid is that 15% (w/v) is stirred continuously in concentrated sulfuric acid adition process；Reflux 2 hours, is cooled to 5-
10 DEG C, using cake filtration, obtain crude extract；Crude extract is added in 85% (v/v) ethyl alcohol of 8 times of weight, and is added
The active carbon of 0.04 times of weight flows back 40 minutes, and filtering obtains filtrate；The pH of filtrate is modulated into 3-4 using dilute hydrochloric acid, is used
Cake filtration after filtration cakes torrefaction, oleanolic acid is removed from filter cake, obtains oleanolic acid using distilled water filter wash cake.
The other components of toothpaste are referred to formulation of tooth-paste realization in the prior art, and details are not described herein again.
The embodiment of the present invention has the advantages that the helicobacter pylori that can be killed or inhibit in oral cavity, so as to avoid
The helicobacter pylori of (such as teeth space, calculus dentalis etc.) constantly enters alimentary canal with saliva in oral cavity, and leads to helicobacter pylori
Relevant disease of digestive tract recurrent exerbation；To overcome conventional therapy helicobacter pylori infections only to stomach and intestine medication, have ignored
This helicobacter pylori of oral cavity aggregation ground, so that the always continuous repeated infection of disease of digestive tract caused by helicobacter pylori, difficult
The problem of to eradicate.
Embodiment 5
A kind of toothpaste of anti-helicobacter pylori is present embodiments provided, the toothpaste contains oleanolic acid, and oleanolic acid is made
For the active constituent of anti-helicobacter pylori.
In toothpaste, the content of oleanolic acid is 16mg/100ml, i.e., contains 16mg oleanolic acid in every 100ml toothpaste.
Oleanolic acid is to extract obtained natural plant extracts from the fruit of glossy privet (fruit of the fruit of glossy privet).Extracting method is such as
Under:
The fruit of glossy privet (fruit of the fruit of glossy privet) is broken, 20 meshes are crossed, medicinal powder is obtained；Medicinal powder is added to 5 times of weight
In 70% (v/v) ethyl alcohol, refluxing extraction at least 3 times, each used time 2.5-3.5 hours, alcohol extract merged；Distillation removal ethyl alcohol,
Then the concentrated sulfuric acid is added, until the concentration of sulfuric acid is that 15% (w/v) is stirred continuously in concentrated sulfuric acid adition process；It flows back 2 small
When, it is cooled to 5-10 DEG C, using cake filtration, obtains crude extract；Crude extract is added to 85% (v/v) second of 8 times of weight
In alcohol, and the active carbon of 0.04 times of weight is added, flows back 40 minutes, filtering obtains filtrate；Using dilute hydrochloric acid by the pH of filtrate
Modulation 3-4, using distilled water filter wash cake, after filtration cakes torrefaction, oleanolic acid is removed from filter cake, is obtained using cake filtration
To oleanolic acid.
The other components of toothpaste are referred to formulation of tooth-paste realization in the prior art, and details are not described herein again.
The embodiment of the present invention has the advantages that the helicobacter pylori that can be killed or inhibit in oral cavity, so as to avoid
The helicobacter pylori of (such as teeth space, calculus dentalis etc.) constantly enters alimentary canal with saliva in oral cavity, and leads to helicobacter pylori
Relevant disease of digestive tract recurrent exerbation；To overcome conventional therapy helicobacter pylori infections only to stomach and intestine medication, have ignored
This helicobacter pylori of oral cavity aggregation ground, so that the always continuous repeated infection of disease of digestive tract caused by helicobacter pylori, difficult
The problem of to eradicate.
Embodiment 6
A kind of toothpaste of anti-helicobacter pylori is present embodiments provided, the toothpaste contains oleanolic acid, and oleanolic acid is made
For the active constituent of anti-helicobacter pylori.
In toothpaste, the content of oleanolic acid is 16mg/100ml, i.e., contains 16mg oleanolic acid in every 100ml toothpaste.
Oleanolic acid is to extract to obtain natural plant extracts from mussot swertia herb.Extracting method is as follows:
Mussot swertia herb is crushed, 20 meshes is crossed, obtains medicinal powder；Medicinal powder is added in the water of 20 times of weight, decocts 3
It is secondary, 1 hour every time, merge decocting liquid；Decocting liquid is concentrated to dilute cream shape, the concentrated sulfuric acid is added, until the concentration of sulfuric acid is 20% (w/
V), it in concentrated sulfuric acid adition process, is stirred continuously；2-5 times of water dilution is added, uses cake filtration；Filter cake is washed, is then made
It is boiled filter cake 2 times with 3% (w/v) sodium hydroxide solution, the liquid after boiling every time filters filter cake again, reuses boiling boiling filter
Cake, the liquid boiled filter filter cake again；Obtain crude extract；Crude extract is added in 85% (v/v) ethyl alcohol of 8 times of weight, and
The active carbon of 0.04 times of weight is added, flows back 40 minutes, filtering obtains filtrate；The pH of filtrate is modulated into 3-4 using dilute hydrochloric acid,
Using cake filtration, using distilled water filter wash cake, after filtration cakes torrefaction, oleanolic acid is removed from filter cake.
The other components of toothpaste are referred to formulation of tooth-paste realization in the prior art, and details are not described herein again.
The embodiment of the present invention has the advantages that the helicobacter pylori that can be killed or inhibit in oral cavity, so as to avoid
The helicobacter pylori of (such as teeth space, calculus dentalis etc.) constantly enters alimentary canal with saliva in oral cavity, and leads to helicobacter pylori
Relevant disease of digestive tract recurrent exerbation；To overcome conventional therapy helicobacter pylori infections only to stomach and intestine medication, have ignored
This helicobacter pylori of oral cavity aggregation ground, so that the always continuous repeated infection of disease of digestive tract caused by helicobacter pylori, difficult
The problem of to eradicate.
Embodiment 7
A kind of toothpaste of anti-helicobacter pylori is present embodiments provided, the toothpaste contains oleanolic acid, and oleanolic acid is made
For the active constituent of anti-helicobacter pylori.
In toothpaste, the content of oleanolic acid is 16mg/100ml, i.e., contains 16mg oleanolic acid in every 100ml toothpaste.
Oleanolic acid is to extract to obtain natural plant extracts from arched bottom.Extracting method is as follows:
Arched bottom is crushed, 20 meshes is crossed, obtains medicinal powder；Medicinal powder is added in the water of 20 times of weight, decoction 3 times, often
Secondary 1 hour, merge decocting liquid；Decocting liquid is concentrated to dilute cream shape, the concentrated sulfuric acid is added, until the concentration of sulfuric acid is 20% (w/v),
In concentrated sulfuric acid adition process, it is stirred continuously；2-5 times of water dilution is added, uses cake filtration；Filter cake is washed, then uses 3%
(w/v) sodium hydroxide solution boils filter cake 2 times, and the liquid after boiling every time filters filter cake again, reuses boiling boiling filter cake, boils
The liquid of boiling filters filter cake again；Obtain crude extract；Crude extract is added in 85% (v/v) ethyl alcohol of 8 times of weight, and is added
The active carbon of 0.04 times of weight flows back 40 minutes, and filtering obtains filtrate；The pH of filtrate is modulated into 3-4 using dilute hydrochloric acid, is used
After filtration cakes torrefaction, oleanolic acid is removed from filter cake using distilled water filter wash cake for cake filtration.
The other components of toothpaste are referred to formulation of tooth-paste realization in the prior art, and details are not described herein again.
The embodiment of the present invention has the advantages that the helicobacter pylori that can be killed or inhibit in oral cavity, so as to avoid
The helicobacter pylori of (such as teeth space, calculus dentalis etc.) constantly enters alimentary canal with saliva in oral cavity, and leads to helicobacter pylori
Relevant disease of digestive tract recurrent exerbation；To overcome conventional therapy helicobacter pylori infections only to stomach and intestine medication, have ignored
This helicobacter pylori of oral cavity aggregation ground, so that the always continuous repeated infection of disease of digestive tract caused by helicobacter pylori, difficult
The problem of to eradicate.
Embodiment 8
A kind of toothpaste of anti-helicobacter pylori is present embodiments provided, the toothpaste contains oleanolic acid, and oleanolic acid is made
For the active constituent of anti-helicobacter pylori.
In toothpaste, the content of oleanolic acid is 16mg/100ml, i.e., contains 16mg oleanolic acid in every 100ml toothpaste.
Oleanolic acid is the natural plant extracts extracted from mussot swertia herb.Extracting method is as follows:
Mussot swertia herb is crushed, 20 meshes is crossed, obtains medicinal powder；Medicinal powder is added in the water of 20 times of weight, decocts 3
It is secondary, 1 hour every time, merge decocting liquid；Decocting liquid is concentrated to dilute cream shape, the concentrated sulfuric acid is added, until the concentration of sulfuric acid is 20% (w/
V), it in concentrated sulfuric acid adition process, is stirred continuously；2-5 times of water dilution is added, uses cake filtration；Filter cake is washed, is then made
It is boiled filter cake 2 times with 3% (w/v) sodium hydroxide solution, the liquid after boiling every time filters filter cake again, reuses boiling boiling filter
Cake, the liquid boiled filter filter cake again；Obtain crude extract；Crude extract is added in 85% (v/v) ethyl alcohol of 8 times of weight, and
The active carbon of 0.04 times of weight is added, flows back 40 minutes, filtering obtains filtrate；The pH of filtrate is modulated into 3-4 using dilute hydrochloric acid,
Using cake filtration, using distilled water filter wash cake, after filtration cakes torrefaction, oleanolic acid is removed from filter cake.
The other components of toothpaste are referred to formulation of tooth-paste realization in the prior art, and details are not described herein again.
The embodiment of the present invention has the advantages that the helicobacter pylori that can be killed or inhibit in oral cavity, so as to avoid
The helicobacter pylori of (such as teeth space, calculus dentalis etc.) constantly enters alimentary canal with saliva in oral cavity, and leads to helicobacter pylori
Relevant disease of digestive tract recurrent exerbation；To overcome conventional therapy helicobacter pylori infections only to stomach and intestine medication, have ignored
This helicobacter pylori of oral cavity aggregation ground, so that the always continuous repeated infection of disease of digestive tract caused by helicobacter pylori, difficult
The problem of to eradicate.
Embodiment 9
A kind of toothpaste of anti-helicobacter pylori is present embodiments provided, the toothpaste contains oleanolic acid and Paeonol, institute
State the active constituent of oleanolic acid and Paeonol joint as anti-helicobacter pylori.
Content, the adduction of the content of oleanolic acid of Paeonol are 16mg/100ml.Specifically, containing in every 100ml toothpaste
There are 12mg oleanolic acid, 4mg Paeonol.
Oleanolic acid in the present embodiment is oleanolic acid prepared by embodiment 2.
Paeonol in the present embodiment is the natural plant extracts extracted from moutan root bark.Preparation method will be male
Red root skin crosses 20 meshes, obtains medicinal powder；Medicinal powder is placed in distillation extractor, 15 times that quality is material quality are added
Water is decocted and is extracted 10 hours, is collected distillate and was filtered using vortice vortex oscillation 2 hours, and obtained using qualitative filter paper
Filtrate；Wherein, in vortex oscillation and filtering, temperature is maintained at 60 DEG C or more；Filtrate freeze-drying, obtains Paeonol.
The other components of toothpaste are referred to formulation of tooth-paste realization in the prior art, and details are not described herein again.
The embodiment of the present invention has the advantages that the helicobacter pylori that can be killed or inhibit in oral cavity, so as to avoid
The helicobacter pylori of (such as teeth space, calculus dentalis etc.) constantly enters alimentary canal with saliva in oral cavity, and leads to helicobacter pylori
Relevant disease of digestive tract recurrent exerbation；To overcome conventional therapy helicobacter pylori infections only to stomach and intestine medication, have ignored
This helicobacter pylori of oral cavity aggregation ground, so that the always continuous repeated infection of disease of digestive tract caused by helicobacter pylori, difficult
The problem of to eradicate.
Embodiment 10
A kind of toothpaste of anti-helicobacter pylori is present embodiments provided, the toothpaste contains oleanolic acid and Paeonol, institute
State the active constituent of oleanolic acid and Paeonol joint as anti-helicobacter pylori.
Content, the adduction of the content of oleanolic acid of Paeonol are 16mg/100ml.Specifically, containing in every 100ml toothpaste
There are 8mg oleanolic acid, 8mg Paeonol.
Oleanolic acid in the present embodiment is oleanolic acid prepared by embodiment 3.
Paeonol in the present embodiment is the natural plant extracts extracted from paniculate swallowwort.Preparation method is long by Xu
Minister in ancient times crosses 20 meshes, obtains medicinal powder；Medicinal powder is placed in distillation extractor, 15 times of the water that quality is material quality is added, decocts
Extraction 10 hours is boiled, distillate is collected and was filtered using qualitative filter paper using vortice vortex oscillation 2 hours, and obtained filtrate；
Wherein, in vortex oscillation and filtering, temperature is maintained at 60 DEG C or more；Filtrate freeze-drying, obtains Paeonol.
The other components of toothpaste are referred to formulation of tooth-paste realization in the prior art, and details are not described herein again.
The embodiment of the present invention has the advantages that the helicobacter pylori that can be killed or inhibit in oral cavity, so as to avoid
The helicobacter pylori of (such as teeth space, calculus dentalis etc.) constantly enters alimentary canal with saliva in oral cavity, and leads to helicobacter pylori
Relevant disease of digestive tract recurrent exerbation；To overcome conventional therapy helicobacter pylori infections only to stomach and intestine medication, have ignored
This helicobacter pylori of oral cavity aggregation ground, so that the always continuous repeated infection of disease of digestive tract caused by helicobacter pylori, difficult
The problem of to eradicate.
Embodiment 11
A kind of toothpaste of anti-helicobacter pylori is present embodiments provided, the toothpaste contains oleanolic acid and Paeonol, institute
State the active constituent of oleanolic acid and Paeonol joint as anti-helicobacter pylori.
Content, the adduction of the content of oleanolic acid of Paeonol are 16mg/100ml.Specifically, containing in every 100ml toothpaste
There are 4mg oleanolic acid, 12mg Paeonol.
Oleanolic acid in the present embodiment is oleanolic acid prepared by embodiment 4.
Paeonol in the present embodiment is the natural plant extracts extracted from moutan root bark.Preparation method will be male
Red root skin crosses 20 meshes, obtains medicinal powder；Medicinal powder is placed in distillation extractor, 15 times that quality is material quality are added
Water is decocted and is extracted 10 hours, is collected distillate and was filtered using vortice vortex oscillation 2 hours, and obtained using qualitative filter paper
Filtrate；Wherein, in vortex oscillation and filtering, temperature is maintained at 60 DEG C or more；Filtrate freeze-drying, obtains Paeonol.
The other components of toothpaste are referred to formulation of tooth-paste realization in the prior art, and details are not described herein again.
The embodiment of the present invention has the advantages that the helicobacter pylori that can be killed or inhibit in oral cavity, so as to avoid
The helicobacter pylori of (such as teeth space, calculus dentalis etc.) constantly enters alimentary canal with saliva in oral cavity, and leads to helicobacter pylori
Relevant disease of digestive tract recurrent exerbation；To overcome conventional therapy helicobacter pylori infections only to stomach and intestine medication, have ignored
This helicobacter pylori of oral cavity aggregation ground, so that the always continuous repeated infection of disease of digestive tract caused by helicobacter pylori, difficult
The problem of to eradicate.
Embodiment 12
A kind of toothpaste of anti-helicobacter pylori is present embodiments provided, the toothpaste contains oleanolic acid and Paeonol, institute
State the active constituent of oleanolic acid and Paeonol joint as anti-helicobacter pylori.
Content, the adduction of the content of oleanolic acid of Paeonol are 16mg/100ml.Specifically, containing in every 100ml toothpaste
There are 8mg oleanolic acid, 8mg Paeonol.
Oleanolic acid in the present embodiment is oleanolic acid prepared by embodiment 5.
Paeonol in the present embodiment is the natural plant extracts extracted from paniculate swallowwort.Preparation method is long by Xu
Minister in ancient times crosses 20 meshes, obtains medicinal powder；Medicinal powder is placed in distillation extractor, 15 times of the water that quality is material quality is added, decocts
Extraction 10 hours is boiled, distillate is collected and was filtered using qualitative filter paper using vortice vortex oscillation 2 hours, and obtained filtrate；
Wherein, in vortex oscillation and filtering, temperature is maintained at 60 DEG C or more；Filtrate freeze-drying, obtains Paeonol.
The other components of toothpaste are referred to formulation of tooth-paste realization in the prior art, and details are not described herein again.
The embodiment of the present invention has the advantages that the helicobacter pylori that can be killed or inhibit in oral cavity, so as to avoid
The helicobacter pylori of (such as teeth space, calculus dentalis etc.) constantly enters alimentary canal with saliva in oral cavity, and leads to helicobacter pylori
Relevant disease of digestive tract recurrent exerbation；To overcome conventional therapy helicobacter pylori infections only to stomach and intestine medication, have ignored
This helicobacter pylori of oral cavity aggregation ground, so that the always continuous repeated infection of disease of digestive tract caused by helicobacter pylori, difficult
The problem of to eradicate.
Embodiment 13
A kind of toothpaste of anti-helicobacter pylori is present embodiments provided, the toothpaste contains oleanolic acid and Paeonol, institute
State the active constituent of oleanolic acid and Paeonol joint as anti-helicobacter pylori.
Content, the adduction of the content of oleanolic acid of Paeonol are 8mg/100ml.Specifically, containing in every 100ml toothpaste
There are 4mg oleanolic acid, 4mg Paeonol.
Oleanolic acid in the present embodiment is oleanolic acid prepared by embodiment 2.
Paeonol in the present embodiment is the natural plant extracts extracted from paniculate swallowwort.Preparation method is long by Xu
Minister in ancient times crosses 20 meshes, obtains medicinal powder；Medicinal powder is placed in distillation extractor, 15 times of the water that quality is material quality is added, decocts
Extraction 10 hours is boiled, distillate is collected and was filtered using qualitative filter paper using vortice vortex oscillation 2 hours, and obtained filtrate；
Wherein, in vortex oscillation and filtering, temperature is maintained at 60 DEG C or more；Filtrate freeze-drying, obtains Paeonol.
The other components of toothpaste are referred to formulation of tooth-paste realization in the prior art, and details are not described herein again.
The embodiment of the present invention has the advantages that the helicobacter pylori that can be killed or inhibit in oral cavity, so as to avoid
The helicobacter pylori of (such as teeth space, calculus dentalis etc.) constantly enters alimentary canal with saliva in oral cavity, and leads to helicobacter pylori
Relevant disease of digestive tract recurrent exerbation；To overcome conventional therapy helicobacter pylori infections only to stomach and intestine medication, have ignored
This helicobacter pylori of oral cavity aggregation ground, so that the always continuous repeated infection of disease of digestive tract caused by helicobacter pylori, difficult
The problem of to eradicate.
Embodiment 14
The antibacterial experiment in vitro of oleanolic acid and the In Vitro Bacteriostasis of oleanolic acid joint Paeonol are carried out in the present embodiment
Experiment.
Oleanolic acid is weighed in 1.5ml centrifuge tube, is dissolved to 2mg/ml using dimethyl sulfoxide (DMSO), is used
Membrane filtration degerming obtains oleanolic acid mother liquor.
Oleanolic acid and Paeonol are mixed according to weight ratio 1:1, obtain the first combination.First combination is weighed
In 1.5ml centrifuge tube, dimethyl sulfoxide (DMSO) is dissolved to 2mg/ml, using membrane filtration degerming, obtains the first combination
Mother liquor.
Oleanolic acid and Paeonol are mixed according to weight ratio 3:1, obtain the second combination.First combination is weighed
In 1.5ml centrifuge tube, dimethyl sulfoxide (DMSO) is dissolved to 2mg/ml, using membrane filtration degerming, obtains the second combination
Mother liquor.
Oleanolic acid and Paeonol are mixed according to weight ratio 1:3, obtain third combination.Third combination is weighed
In 1.5ml centrifuge tube, dimethyl sulfoxide (DMSO) is dissolved to 2mg/ml, using membrane filtration degerming, obtains third combination
Mother liquor.
On 96- microwell plate using dimethyl sulfoxide (DMSO) to oleanolic acid mother liquor, compound joint mother liquor respectively into
The gradient dilution that 2 times of row successively obtains the work of 20,1,0.5,0.25,0.125,0.0625,0.03125,0.0156mg/ml
Liquid.
A kind of 100 microlitres of above-mentioned working solutions (centrifuge tube adds working solution) are added in 50ml centrifuge tube, are then added
50-55 DEG C of the MHA (Mueller Hinton Agar) that 25ml not yet condenses heals culture medium, falls to make immediately after quickly mixing
Plate, be made the plate of the 8 μ g/ml containing oleanolic acid, the plate of the 4 μ g/ml containing oleanolic acid, the 2 μ g/ml containing oleanolic acid it is flat
Plate, the plate of the 1 μ g/ml containing oleanolic acid, the plate of the 0.5 μ g/ml containing oleanolic acid, the 0.25 μ g/ml containing oleanolic acid it is flat
Plate, the plate of the 0.125 μ g/ml containing oleanolic acid, the 0.0625 μ g/ml containing oleanolic acid plate, the 8 μ g/ containing the first combination
The plate of ml, the plate of the 4 μ g/ml containing the first combination, the plate of the 2 μ g/ml containing the first combination, the 1 μ g/ containing the first combination
The plate of ml, the plate of the 0.5 μ g/ml containing the first combination, the 0.25 μ g/ml containing the first combination plate, contain the first combination
The plate of the plate of 0.125 μ g/ml, the 0.0625 μ g/ml containing the first combination, the plate of the 8 μ g/ml containing the second combination, containing the
The plate of two combinations, 4 μ g/ml, the plate of the 2 μ g/ml containing the second combination, the plate of the 1 μ g/ml containing the second combination, containing the
The plate of two combinations, 0.5 μ g/ml, the plate of the 0.25 μ g/ml containing the second combination, the 0.125 μ g/ml containing the second combination
The plate of plate, the 0.0625 μ g/ml containing the second combination, plate, the 4 μ g/ of combination containing third of 8 μ g/ml of combination containing third
The plate of ml, the plate of 2 μ g/ml of combination containing third, the plate of 1 μ g/ml of combination containing third, 0.5 μ of combination containing third
The plate of g/ml, the plate of 0.25 μ g/ml of combination containing third, 0.125 μ g/ml of combination containing third plate, containing third join
Close the plate of 0.0625 μ g/ml of object.
1-3 block processed contains only the MHA blood plate of solvent (dimethyl sulfoxide) simultaneously.
Hereafter for sake of convenience, by the above-mentioned plate containing oleanolic acid, the plate containing combination and solvent is contained only
The MHA blood plate of (dimethyl sulfoxide) is referred to as test plate.
Helicobacter pylori reference culture in -80 DEG C of cryopreservation tubes (three kinds of reference cultures have been used into, respectively altogether
ATCC43504, ATCC700392, ATCC700824) being coated on MHA blood plate, (the MHA blood plate made that falls herein is not above
Test plate, but conventional use of MHA blood plate).The MHA blood plate for being coated with helicobacter pylori reference culture is set
In closing culture box, and placing matched micro- oxygen consumption oxygen uptake packet makes it generate stable micro- oxygen-depleted environment, and culture box is put in
It is cultivated 5 days at a temperature of 35 ± 2 DEG C.
After 5 days, the bacterium on MHA blood plate is collected, and is suspended in the physiological saline of pH buffering, turbidity is adjusted to 0.45-
0.6, at this point, bacterium solution contains 1.0 × 107-1.0×108CFU/ml。
Take the 3 microlitres of bacterium solution prepared points on the test plate of above-mentioned preparation, by low concentration (or without compound
Plate) starting point is to high concentration.Compound herein refers to above-mentioned combination or oleanolic acid.
After drop air-dries slightly, test plate is inverted in micro- oxygen consumption culture box, mating oxygen uptake packet is put into, in 35
It is cultivated 72 hours at a temperature of ± 2 DEG C.
It should be noted that experiment can be using Amoxicillin or clarithromycin as positive control.
It after 72 hours, reads minimal inhibitory concentration (MIC), MIC complete inhibition helicobacter pylori grows on plate
Minimum compound concentration.If MIC is less than or equal to the minimum compound concentration (0.0625 μ g/ml) of preparation, which must
Growth must be shown on the plate of not drug containing, and failure otherwise is judged as the test of the strain, MIC result not uses,
It need to repeat to test.
In the present embodiment, respectively there is bacterium growth on test plate, do not read MIC.
Embodiment 15
The antibacterial experiment in vitro and oleanolic acid for carrying out oleanolic acid again in the present embodiment combine the external of Paeonol
Bacteriostatic experiment.
Oleanolic acid is weighed in 1.5ml centrifuge tube, is dissolved to 2mg/ml using dimethyl sulfoxide (DMSO), is used
Membrane filtration degerming obtains oleanolic acid mother liquor.
Oleanolic acid and Paeonol are mixed according to weight ratio 1:1, obtain the first combination.First combination is weighed
In 1.5ml centrifuge tube, dimethyl sulfoxide (DMSO) is dissolved to 400mg/ml, using membrane filtration degerming, obtains the first joint
Object mother liquor.
Oleanolic acid and Paeonol are mixed according to weight ratio 3:1, obtain the second combination.First combination is weighed
In 1.5ml centrifuge tube, dimethyl sulfoxide (DMSO) is dissolved to 400mg/ml, using membrane filtration degerming, obtains the second joint
Object mother liquor.
Oleanolic acid and Paeonol are mixed according to weight ratio 1:3, obtain third combination.Third combination is weighed
In 1.5ml centrifuge tube, dimethyl sulfoxide (DMSO) is dissolved to 400mg/ml, using membrane filtration degerming, obtains third joint
Object mother liquor.
On 96- microwell plate using dimethyl sulfoxide (DMSO) to oleanolic acid mother liquor, compound joint mother liquor respectively into
The gradient dilution that 2 times of row successively obtains the working solution of 400,200,100,50,25,12.5,6.25,3.15mg/ml.
A kind of 100 microlitres of above-mentioned working solutions (centrifuge tube adds working solution) are added in 50ml centrifuge tube, are then added
50-55 DEG C of the MHA (Mueller Hinton Agar) that 25ml not yet condenses heals culture medium, falls to make immediately after quickly mixing
The plate of the 160 μ g/ml containing oleanolic acid, the plate of the 80 μ g/ml containing oleanolic acid, the 40 μ g/ml containing oleanolic acid is made in plate
Plate, the plate of the 20 μ g/ml containing oleanolic acid, the plate of the 10 μ g/ml containing oleanolic acid, the 5 μ g/ml containing oleanolic acid it is flat
Plate, the plate of the 2.5 μ g/ml containing oleanolic acid, the 1.25 μ g/ml containing oleanolic acid plate, the 160 μ g/ml containing the first combination
Plate, the plate of the 80 μ g/ml containing the first combination, the plate of the 40 μ g/ml containing the first combination, the 20 μ g/ containing the first combination
The plate of ml, the plate of the 10 μ g/ml containing the first combination, the plate of the 5 μ g/ml containing the first combination, 2.5 μ containing the first combination
The plate of the plate of g/ml, 1.25 μ g/ml of the first combination, the plate of the 160 μ g/ml containing the second combination contain the second combination
The plate of 80 μ g/ml, the plate of the 40 μ g/ml containing the second combination, the 20 μ g/ml containing the second combination plate, contain second
Close the plate of 10 μ g/ml of object, the plate of the 5 μ g/ml containing the second combination, the plate of the 2.5 μ g/ml containing the second combination, second
Close the plate of 1.25 μ g/ml of object, the plate of 160 μ g/ml of combination containing third, 80 μ g/ml of combination containing third plate, contain
The plate of 40 μ g/ml of third combination, the plate of 20 μ g/ml of combination containing third, 10 μ g/ml of combination containing third plate,
The plate of 5 μ g/ml of combination containing third, the plate of 2.5 μ g/ml of combination containing third, 1.25 μ g/ml of third combination it is flat
Plate.
1-3 block processed contains only the MHA blood plate of solvent (dimethyl sulfoxide) simultaneously.
Hereafter for sake of convenience, by the above-mentioned plate containing oleanolic acid, the plate containing combination and solvent is contained only
The MHA blood plate of (dimethyl sulfoxide) is referred to as test plate.
Helicobacter pylori reference culture in -80 DEG C of cryopreservation tubes (three kinds of reference cultures have been used into, respectively altogether
ATCC43504, ATCC700392, ATCC700824) being coated on MHA blood plate, (the MHA blood plate made that falls herein is not above
Test plate, but conventional use of MHA blood plate).The MHA blood plate for being coated with helicobacter pylori reference culture is set
In closing culture box, and placing matched micro- oxygen consumption oxygen uptake packet makes it generate stable micro- oxygen-depleted environment, and culture box is put in
It is cultivated 5 days at a temperature of 35 ± 2 DEG C.
After 5 days, the bacterium on MHA blood plate is collected, and is suspended in the physiological saline of pH buffering, turbidity is adjusted to 0.45-
0.6, at this point, bacterium solution contains 1.0 × 107-1.0×108CFU/ml。
Take the 3 microlitres of bacterium solution prepared points on the test plate of above-mentioned preparation, by low concentration (or without compound
Plate) starting point is to high concentration.Compound herein refers to above-mentioned combination or oleanolic acid.
After drop air-dries slightly, test plate is inverted in micro- oxygen consumption culture box, mating oxygen uptake packet is put into, in 35
It is cultivated 72 hours at a temperature of ± 2 DEG C.
It should be noted that experiment can be using Amoxicillin or clarithromycin as positive control.
It after 72 hours, reads minimal inhibitory concentration (MIC), MIC complete inhibition helicobacter pylori grows on plate
Minimum compound concentration.If MIC is less than or equal to the minimum compound concentration (0.0625 μ g/ml) of preparation, which must
Growth must be shown on the plate of not drug containing, and failure otherwise is judged as the test of the strain, MIC result not uses,
It need to repeat to test.
In the present embodiment, the plate of the 160 μ g/ml containing oleanolic acid, the 160 μ g/ml containing the first combination plate, contain
Plate, the plate containing the 3rd 160 μ g/ml of second combination, 160 μ g/ml does not have bacterium growth, it is known that, oleanolic acid, first
Close object, the second combination, third combination MIC be 16mg/100ml.
Embodiment 16
In the present embodiment, the antibiotic effect of the anti-helicobacter pylori toothpaste of the offer of embodiment 1-13 is studied.
140 patients w ith peptic ulcer diseases are chosen as reagent volunteer, which is to assert to suffer from pylorus spiral shell through clinic
The patient of gastric ulcer caused by bacillus.Each patients w ith peptic ulcer disease13C urease breath test is positive, and has following one or more
Symptom: upper abdomen is glutted after food, upper abdomen pain, abdominal distension, sour regurgitation, belch, nausea, burning after epigastric discomfort, food after food
The heart, pectoralgia, spitting blood, appetite stimulator, melena, a small amount of bleeding of upper digestive tract etc..
And the non-following situations of each patient:
1, severe cardiac, Liver and kidney function impairer；
2, gestation or breast feeding women；
3, there are bleeding, perforation, pyloric stenosis, cancer complications；
4, there are oesophagus, gastrointestinal procedures history person；
5, patient cannot correctly express the main suit of oneself, such as mental disease, serious neurological functional disease person；
6, patient takes non-steroidal anti-inflammatory drugs or excessive drinking simultaneously；
7, to penicillin drug allergy person.
14 groups are randomly divided into, successively first group, second group, third group, the 4th group, the 4th group, the 5th group, the 6th group,
Seven groups, the 8th group, the 9th group, the tenth group, the 11st group, the 12nd group, the 13rd group, control group, every group of 10 people.
The above each group is carried out according to tetrad (bismuth agent+proton pump inhibitor+tetracycline+metronidazole) 14 days treatment methods
Treatment.While using quadruple therapy, first group of toothpaste provided using embodiment 1 is brushed teeth each primary sooner or later；Second group makes
The toothpaste provided with embodiment 2 is brushed teeth each primary sooner or later；The toothpaste that third group is provided using embodiment 3 is brushed teeth each primary sooner or later；
The 4th group of toothpaste provided using embodiment 4 is brushed teeth each primary sooner or later；The 5th group of toothpaste provided using embodiment 5 is brushed sooner or later
Tooth is each primary；The 6th group of toothpaste provided using embodiment 6 is brushed teeth each primary sooner or later；The 7th group of tooth provided using embodiment 7
Cream is brushed teeth each primary sooner or later；The 8th group of toothpaste provided using embodiment 8 is brushed teeth each primary sooner or later；9th group uses embodiment 9
The toothpaste of offer is brushed teeth each primary sooner or later；Tenth group of toothpaste provided using embodiment 10 brush teeth sooner or later it is each it is primary, the 11st group
The toothpaste that is there is provided using embodiment 11 is brushed teeth each primary sooner or later, and the 12nd group of toothpaste provided using embodiment 12 is brushed teeth sooner or later
Each primary, the 13rd group of toothpaste provided using embodiment 13 is brushed teeth each primary sooner or later.
The irritable foods such as smoking ban, wine, tea, pungent and injury of gastrointestinal tract drug during treatment.After drug withdrawal 4th week and
Patient is carried out twice within 8th week13Effect is eradicated in the confirmation of C urease breath test.The results are shown in Table 1.
As known from Table 1, when content >=16mg/100ml of the oleanolic acid in toothpaste or oleanolic acid and Paeonol are total
When content >=16mg/100ml, cooperates 14 days therapeutic schemes of tetrad, the very elimination of helicobacter pylori may be implemented；When
When content is 8mg/100ml, 90 percent elimination can achieve；And only use tetrad 14 days, eradication rate then only has percentage
70.
Therefore, toothpaste provided in an embodiment of the present invention cooperates existing Eradication Therapy of Helicobacter pylori Infection scheme, can significantly improve
The eradication rate of helicobacter pylori.
Although above having used general explanation and specific embodiment, the present invention is described in detail, at this
On the basis of invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore,
These modifications or improvements without departing from theon the basis of the spirit of the present invention are fallen within the scope of the claimed invention.
Claims (10)
1. a kind of dental care products of anti-helicobacter pylori, which is characterized in that the dental care products contain oleanolic acid,
Active constituent of the oleanolic acid as anti-helicobacter pylori.
2. dental care products according to claim 1, which is characterized in that in the dental care products, olive
Content >=16mg/100ml of acid.
3. dental care products according to claim 1, which is characterized in that the oleanolic acid in the dental care products
For the first natural plant extracts；The raw material for preparing of first natural plant extracts includes following any one or more:
Olive leaf, arched bottom, the fruit of glossy privet, mussot swertia herb；
First natural extract is prepared by the first oleanolic acid extracting method or the second oleanolic acid extracting method:
First oleanolic acid extracting method includes: by one of olive leaf, arched bottom, the fruit of glossy privet, mussot swertia herb or more
Kind is broken, crosses 20 meshes, obtains medicinal powder；Medicinal powder is added in 70% (v/v) ethyl alcohol of 5 times of weight, refluxing extraction at least 3
Secondary, each used time 2.5-3.5 hours, alcohol extract merged；Distillation removal ethyl alcohol, is then added the concentrated sulfuric acid, until the concentration of sulfuric acid is
15% (w/v) is stirred continuously in concentrated sulfuric acid adition process；Reflux 2 hours, is cooled to 5-10 DEG C, using cake filtration, obtains
To crude extract；Crude extract is added in 85% (v/v) ethyl alcohol of 8 times of weight, and the active carbon of 0.04 times of weight is added, reflux
40 minutes, filtering obtained filtrate；The pH modulation 3-4 of filtrate is used into distilled water filter wash using cake filtration using dilute hydrochloric acid
Cake removes oleanolic acid after filtration cakes torrefaction from filter cake；
Second oleanolic acid extracting method is the following steps are included: by olive leaf, arched bottom, the fruit of glossy privet, mussot swertia herb
It is one or more broken, 20 meshes are crossed, medicinal powder is obtained；Medicinal powder is added in the water of 20 times of weight, is decocted 3 times, 1 is small every time
When, merge decocting liquid；Decocting liquid is concentrated to dilute cream shape, the concentrated sulfuric acid is added, until the concentration of sulfuric acid is 20% (w/v), in the concentrated sulfuric acid
In adition process, it is stirred continuously；2-5 times of water dilution is added, uses cake filtration；Filter cake is washed, 3% (w/v) hydrogen is then used
Sodium hydroxide solution is boiled filter cake 2 times, and the liquid after boiling every time filters filter cake again, reuses boiling boiling filter cake, the liquid boiled
Filter cake is filtered again；Obtain crude extract；Crude extract is added in 85% (v/v) ethyl alcohol of 8 times of weight, and 0.04 times of weight is added
Active carbon, flow back 40 minutes, filtering, obtain filtrate；The pH modulation 3-4 of filtrate is made using cake filtration using dilute hydrochloric acid
Oleanolic acid is removed from filter cake after filtration cakes torrefaction with distilled water filter wash cake.
4. a kind of dental care products of anti-helicobacter pylori, which is characterized in that the dental care products contain oleanolic acid
And Paeonol, oleanolic acid and Paeonol combine the active constituent as anti-helicobacter pylori.
5. dental care products according to claim 4, which is characterized in that the root bark of tree peony in the dental care products
Phenol is the second natural plant extracts；
The raw material for preparing of second natural plant extracts includes:
Moutan root bark and/or paniculate swallowwort；
Second natural extract is prepared by the following method to obtain:
Moutan root bark and/or paniculate swallowwort are crushed, 20 meshes is crossed, obtains medicinal powder；
Medicinal powder is placed in distillation extractor, 15 times of the water that quality is medicinal powder quality is added, decocts and extracts 10 hours, collection evaporates
Liquid out was filtered using qualitative filter paper using vortice vortex oscillation 2 hours, and was obtained filtrate；Wherein, in vortex oscillation and mistake
When filter, temperature is maintained at 60 DEG C or more；
Filtrate freeze-drying, obtains Paeonol.
6. dental care products according to claim 4, which is characterized in that in the dental care products, olive
Acid content, the content of Paeonol and >=16mg/100ml.
7. dental care products according to claim 6, which is characterized in that in the dental care products, olive
The weight ratio of acid and Paeonol is 1:99-99:1.
8. dental care products according to claim 7, which is characterized in that in the dental care products, olive
The weight ratio of acid and Paeonol is 50:50.
9. purposes of the oleanolic acid in the dental care products for preparing anti-helicobacter pylori.
10. oleanolic acid and Paeonol combine the purposes in the dental care products for preparing anti-helicobacter pylori.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1045585A (en) * | 1990-04-03 | 1990-09-26 | 邵雅旭 | A kind of method of from plant, extracting Oleanolic Acid |
| CN1124653A (en) * | 1995-04-05 | 1996-06-19 | 雷学军 | Natural medicine for resisting pyloric Helicobacterium |
| US20050238740A1 (en) * | 2002-05-01 | 2005-10-27 | Spiros Fotinos | Use of mastic and its components for the control of microbial infections |
| CN102659899A (en) * | 2012-04-24 | 2012-09-12 | 劲牌有限公司 | Method for extracting purified oleanolic acid from glossy privet fruits |
| CN105147650A (en) * | 2015-10-14 | 2015-12-16 | 上海中医药大学 | Medical application of paeonol |
| CN105801392A (en) * | 2016-04-14 | 2016-07-27 | 重庆大学 | Extracting method of 2'-hydroxy-4'-methoxyacetopheone |
-
2018
- 2018-10-30 CN CN201811278710.5A patent/CN109248103A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1045585A (en) * | 1990-04-03 | 1990-09-26 | 邵雅旭 | A kind of method of from plant, extracting Oleanolic Acid |
| CN1124653A (en) * | 1995-04-05 | 1996-06-19 | 雷学军 | Natural medicine for resisting pyloric Helicobacterium |
| US20050238740A1 (en) * | 2002-05-01 | 2005-10-27 | Spiros Fotinos | Use of mastic and its components for the control of microbial infections |
| CN102659899A (en) * | 2012-04-24 | 2012-09-12 | 劲牌有限公司 | Method for extracting purified oleanolic acid from glossy privet fruits |
| CN105147650A (en) * | 2015-10-14 | 2015-12-16 | 上海中医药大学 | Medical application of paeonol |
| CN105801392A (en) * | 2016-04-14 | 2016-07-27 | 重庆大学 | Extracting method of 2'-hydroxy-4'-methoxyacetopheone |
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Application publication date: 20190122 |
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