CN109239239A

CN109239239A - The Chinese medicine composition and its preparation method and purposes of clearing lung, eliminating phlegm, relieving cough & asthma

Info

Publication number: CN109239239A
Application number: CN201811402012.1A
Authority: CN
Inventors: 张海; 范晓波; 董秀
Original assignee: Guizhou Youkean Biotechnology Co Ltd
Current assignee: Guizhou Youkean Biotechnology Co Ltd
Priority date: 2018-11-22
Filing date: 2018-11-22
Publication date: 2019-01-18
Anticipated expiration: 2038-11-22
Also published as: CN109239239B; CN113559207A

Abstract

The present invention relates to Chinese medicine compositions of clearing lung, eliminating phlegm, relieving cough & asthma and its preparation method and application.Specifically, relate in one aspect to a kind of Chinese medicine composition, it is prepared by the Chinese medicine for including radix scutellariae and tabasheer etc. and optional pharmaceutic adjuvant, wherein each medicinal material is in the form of medicinal powder or to be in the form of its extract or be to extract extract obtained form together with other one or more medicinal materials with it to be added in the composition；The Chinese medicine composition is, for example, the forms such as external preparation, such as patch, ointment, cream；The Chinese medicine composition is, for example, oral preparation；Such as the forms such as granule, tablet, capsule, oral solution, electuary, pill, spray.The present invention with a kind of simple Chinese traditional medicine composition to treat cough with lung heat caused by a variety of causes, the illnesss such as phlegm is contained, wheeze, phlegm Huang, red tongue with yellowish fur, can especially be efficiently used for pediatric patient clearing lung, eliminating phlegm, relieving cough & asthma.

Description

The Chinese medicine composition and its preparation method and purposes of clearing lung, eliminating phlegm, relieving cough & asthma

Technical field

The invention belongs to technical field of traditional Chinese medicines, are related to a kind of Chinese medicine composition for clearing lung, eliminating phlegm, relieving cough & asthma, also relate to And prepare the method and these clearing lung, eliminating phlegm, relieving cough & asthma Chinese medicine compositions of the clearing lung, eliminating phlegm, relieving cough & asthma Chinese medicine composition Pharmaceutical applications.More particularly, the present invention a few herbs a small number of using radix scutellariae etc. realize that effective clearing lung and eliminating phlegm cough-relieving is flat Asthma, can especially be used with convenient administration route, such as child patient.

Background technique

Cough is respiratory disease one of the most common type symptom, is a kind of protectiveness defense function of human body.It, can by cough The secretion or the endotracheal foreign matter of intrusion of respiratory tract is discharged, only coughs and be known as dry cough without phlegm, it is seen that in a variety of diseases Disease.There are many reason of cough, for example, chronic pharyngitis: the pharyngeal drying of patient has scratchiness and sense of discomfort, and thus stimulation hair It enters the arena paroxysmal cough sound, oneself can find that pharynx rear wall mucomembranous surface is coarse against mirror lips, and there are many thin vessels expanded, seriously Shi Huiyou transparent little Bai bubble is common with pure.Chronic laryngitis: if patient's dry cough feels throat with hoarseness Drying is itched, smartly pain, it is possible to be laryngitis.Chronic bronchitis: person above middle age often coughs, and brings up or more Or few viscous sputum, aggravate after waking up in the morning, annual winter-spring season breaking-out, summer mitigates or alleviates, and the serious or time is longer Finding case thorax it is broadening, should suspect for chronic bronchitis.Bronchial asthma: if cough recurrent exerbation, larynx when breathing Between such as dragsaw, while patient feels uncomfortable in chest, expiratory dyspnea, every cold season or can induce cough when contacting certain allergin It coughs, should check whether with bronchial asthma.Pulmonary tuberculosis, lung cancer: patient's cough sound is humble, and cough sputum streaked with blood or hemoptysis are felt from head to foot Powerless, appetite is reduced, and body day is gradually thin, and afternoon and night body temperature increases, and perspiration increases during sleep, when have palpitation, while companion There are two cheekbones rubescent.Infectivity cough: respiratory disease such as upper respiratory tract infection or bacterium infection can also cause to cough.It is other Reason cough: the diseases such as foreign body in airpassage, pleurisy, pleural effusion and many physics, chemical factor can also cause to cough.Moistening lung Cough-relieving refers to a kind of therapeutic effect that cough due to yin deficiency and Chinese medicine are treated using Yin nourishing and lung moistening Chinese medicine.

Cough, asthma and sputum often have substantial connection on the interpretation of the cause, onset and process of an illness, and cough and asthma holds phlegm under the arm more, and abundant expectoration often causes cough and asthma.Cough is to exhale One of most common symptom of desorption system disease, it is a kind of protectiveness neural reflex, generates expiration percussion action by cough, Can by respiratory tract foreign matter or secretion excrete.Cough can be divided to dry, wet two kinds.Dry cough, have have dizzy spells, scorching hot sense Feel, and cough does not go out phlegm.It is common in flu, bronchitis and pneumonia initial stage.Long lasting for dry cough, it may be possible to pulmonary tuberculosis. The symptom of wet cough is that cough is not stopped, and throat is with phlegm.Chinese medicine thinks that cough is that lung, which loses, declares respectful, the unclear institute of lung qi because of evil objective lung system It causes.According to theory of traditional Chinese medical science, for the pathogenesis of cough, with clearing heat and detoxicating, moistening lung for removing phlegm, relieving cough and asthma for principle of reatment, into Row dialectical compatibility prescription, to achieve the purpose that treating both manifestation and root cause of disease.

Cough variant asthma is also known as cough variant asthma, refers to one kind with chronic cough for main or only clinical manifestation Specific type asthma.Cough is its solely or mainly clinical symptoms, and nothing is obviously wheezed, but has airway hyperreactivity, main to show For irritation dry cough, nocturnal cough is particularly acute.At present research think this disease be mainly by inflammation medium, cell factor, Caused by inflammatory cell interaction.It additionally include the multi-party factors such as neural factors, infective agent and inherent cause.Traditional medicine Think lung being a delicate viscus, if lung be exopathogen invasion, cannot normally declare hair and respectful drop, the circulation of vital energy in the wrong direction and on, clinical signs be cough, Lung Qi upward reversal, and wind contraction is anxious, feature causes air flue contraction anxious more, and the bronchial spasm for being equivalent to western medicine changes.This disease disease Machine surely belongs to exogenous disease mistake and controls, and heresy is strongly fragrant to lead to Lung Qi obstraction in lung, and lung pipe is unfavorable, and air flue contraction is anxious.Or at disease initial stage, cross with it is cold and cool, Or large dosage of antibiotic causes cold-evil is strongly fragrant to hold back in lung, without door, lung can only receive this dirty gas, cannot receive surname dissipate also intolerant to fever and chills External is courteous, the strongly fragrant lung of perverse trend, and a surname sends out drop functional disturbance respectful, and the tissue morphology of lung pipe also changes therewith, leads to tracheae Spasm.Cough variant asthma belongs to the scopes such as Chinese medicine " cough ", " card of roaring ", " dyspnea ", often in spring and alternating autumn and winter, morbidity time. Mostly because exogenous disease mistake is controlled, the dry lung of perverse trend, the pneumatic circulation of vital energy in the wrong direction, phlegm out of breath resistance and cause.This disease has recurrent exerbation, onset urgency, rapid onset etc. Feature, it is before this onste more there is sneeze, nasopharyngeal airways to itch, runny nose, the premonitory symptoms such as uncomfortable in chest, open and let out with wind being YANG-pathogen, its property The characteristics of be consistent.Wind is first of the six external factors which cause diseases, other exopathogens mostly invade human body with ailment said due to cold or exposure, and exogenous cough is often based on wind, or folder is cold, Or folder heat, or folder it is dry, or folder phlegm, wherein and folder hair patient it is in the majority.Know that ailment said due to cold or exposure is that this disease occurs, in development and evolution process One of main pathogenic, and and folder evil only one of triggering factors rather than internal factor.

In addition, the common disease and frequently-occurring disease of childhood that children's bronchus asthma is, are by eosinophil, mast cell The chronic ill reactive disorder of air flue participated in the inflammations cell such as T lymphocyte and cell factor.And bronchus is roared It can occur when asthma is regardless of season, especially fall ill at season in severe winter and climate temperature change dramatically more.Mostly because of body void It is weak, or touch following factor this disease can be induced to the infant that lung has volt phlegm: meet sense chill.Direct stimulation depression or environment are rapid Become, inhales people's dust and diet etc..Since development in children is not yet mature, premunition is low, belongs to the body of immature yin and yang, and internal organs are tender Tender, shape gas does not fill, and how empty lung spleen is, and easily empty easily real, and strongly fragrant with the passing of time the being easy to heat of phlegm is mostly cold-heat complication, actual situation in clinical manifestation And it deposits.Children's internal organs development also prematurity.It is responsible for due to human skin category lung, lungs are delicate, and it is poor that skin defends outer function, skin Space between skin and muscles is not fine and close, and chill, wind-heat invade human body, kidney deficiency by fur or mouth and nose, and spleen, lung, renal function imbalance cause phlegm retention to be formed, Perverse trend is taken advantage of a weak point, and perverse trend enters lung channel priming volt phlegm, phlegm rising following QI, and obstruction of QI due to retention of phlegm is fought mutually sharp, obstructing airway, ascending or descending movement of vital Qi It is unfavorable so that more suckings of exhalation are lacked, breath syndrome characterized by dyspnea, throat croup phlegm toot.Symptom are as follows: be short of breath, breaking-out is unexpected, sulks Slightly, larynx has asked wheezing sound, must not wheeze and lies down.It generally breaks out in paroxysmal, or agitation, perspiration, expectoration, Celadon purple, complexion occurs Pale, the heavy then symptoms such as obnubilation.

These coughs, asthma or the treatments wheezed such as are usually directed to cough-relieving, resolving sputum, relieving asthma at various symptomatic treatments.West Medicine is mostly treated with compound medicine, however when being used for child for treatment, the potential side effect of Western medicine is usually troubling, and usually examines Consider and is treated using the method for traditional Chinese medicine.

The prior art has the report of many traditional Chinese medicine technologies about clearing lung, eliminating phlegm, relieving cough & asthma.For example, CN107970332A (application number: 201610905181.1, Jiang Shunhua) discloses a kind of drug for treating bronchial asthma, group Be divided into: gekko, caryophyllaceous ginseng, bryozoatum, Fermented Soybean, wide dragon, aster, gleditsia sinensis, Rhizoma Arisaematis (processed), asarum process radix scutellariae, stalactite, mulberry White skin, Schisandra chinensis, agalloch eaglewood, fried mustard seed, almond, radix scutellariae, Os Draconis, Radix Glycyrrhizae；Component ratio are as follows: 6 grams of gekko (removing cephalopodium), caryophyllaceous ginseng 30 grams, 30 grams of bryozoatum (being decocted first), 10 grams of Fermented Soybean, 10 grams of wide dragon, 10 grams of aster, 3 grams of gleditsia sinensis, 6 grams of Rhizoma Arisaematis (processed), asarum 6 Gram, 6 grams of radix scutellariae of toast, 15 grams of stalactite (being decocted first), 10 grams of the root bark of white mulberry, 10 grams of Schisandra chinensis, 6 grams of agalloch eaglewood, 6 grams of fried mustard seed, almond 10 Gram, 10 grams of radix scutellariae, 20 grams of Os Draconis (being decocted first), 6 grams of Radix Glycyrrhizae.

CN107303340A (application number: 201610251388.1, Yang Congbing) provides a kind of Chinese traditional medicine composition for treating asthma Object is made of gingko, radix scutellariae, coltsfoot, the root bark of white mulberry, rhizoma pinellinae praeparata, perillaseed, Radix Glycyrrhizae, almond radix scutellariae, it is believed that the composition has suitable lung Heat-clearing, resolving sputum stop heavy breathing effect, and clinical cure rate is up to 91% or more.

CN108210703A (application number: 201611160874.9, Wu Hongxia) is disclosed and a kind of is treated children's bronchus asthma Chinese medicine composition, be prepared by the raw material of following parts by weight: 3-9 parts of folium artemisiae argyi, 5-10 parts of perilla leaf, 6-12 parts of the root bark of white mulberry, 3-9 parts of green peel, 1-6 parts of Schisandra chinensis, 3-10 parts of almond, 2-9 parts of radix scutellariae, 3-9 parts of ginseng, 9-15 parts of Poria cocos, 3-9 parts of campanulaceae, river 3-9 parts of shellfish, 3-9 parts of peppermint, 6-15 parts of longstalk condorvine herb, 3-9 parts of rhizoma arisaematis and 1-10 parts of Radix Glycyrrhizae.It is believed that the Chinese medicine composition has hair The effect of sweat cold dispelling, official's lung are relievingd asthma, expelling phlegm and arresting coughing, soothing the liver relieving stagnant Qi and clearing heat and moistening lung, also have preparation process simple, good effect, The advantages that side effect is low, convenient to take and application easy to spread.

CN108339046A (application number: 201710040144.3, sky is same) it is related to a kind of Chinese medicine composition for treating cough, Its Chinese medicine material and weight are as follows: 3-18 parts of Radix Angelicae Sinensis, toast radix scutellariae 1-10 parts, 5-25 parts of mustard seed, 3-18 parts of the root bark of white mulberry, tendril-leaved fritillary bulb 3-18 parts female, 3-18 parts of radix scutellariae, 3-18 parts of almond, 3-18 parts of bombyx batryticatus, 5-20 parts of the root of straight ladybell, 1-10 parts of Radix Glycyrrhizae.It is believed that the invention Chinese medicine composition has warming spleen and stomach for dispelling cold, clearing heat and detoxicating, moistening lung for removing phlegm, relieving cough and asthma effect, and treatment cough is significant in efficacy, makees With reliable；Its component medicine source is wide, it is easy to prepare, has no toxic side effect.

CN108452161A (application number: 201710094478.9, Kang Hui) being related to a kind of Chinese medicine composition for treating cough, Its Chinese medicine material and weight are as follows: 3-18 parts of Radix Angelicae Sinensis, toast radix scutellariae 1-10 parts, 5-25 parts of mustard seed, 3-18 parts of the root bark of white mulberry, tendril-leaved fritillary bulb 3-18 parts female, 3-18 parts of radix scutellariae, 3-18 parts of almond, 3-18 parts of bombyx batryticatus, 5-20 parts of the root of straight ladybell, 1-10 parts of Radix Glycyrrhizae.It is believed that the invention Chinese medicine composition has warming spleen and stomach for dispelling cold, clearing heat and detoxicating, moistening lung for removing phlegm, relieving cough and asthma effect, and treatment cough is significant in efficacy, makees With reliable；Its component medicine source is wide, it is easy to prepare, has no toxic side effect.

CN108465012A (application number: 201810663573.0, hundred swords) disclose a kind of treat in bronchial asthma Medicine, the Chinese medicine are formulated by following medicinal material, their quality proportioning are as follows: radix scutellariae 6~10, asarum 3~6, gypsum 20~ 26, ramulus cinnamomi 4~8, Exocarpium Citri Rubrum 10~12, mustard seed 10~12, Radix Astragali 20~30, the root bark of white mulberry 20~30, Fructus Aurantii 10~14, radix aucklandiae 4~ 7, radix bupleuri 10~14, mulberry leaf 10~14, honeysuckle 10~14, tussilago 8~12, loguat leaf 8~12；The radix scutellariae must be fried in advance System, the gypsum is raw to be used.Preparation method is by each taste Chinese medicine in formula for medicine, wherein radix scutellariae was processed in advance；Again by above-mentioned whole medicines Object drying, broken disinfection adds water to decoct repeatedly 3 times in flakes, filters and is made into medicinal extract；Fire drying in using again crushes, pulverizes End；Finally be pressed into tablet or be packed into capsule to get.It is believed that the Chinese Herbs of the invention are rapidly, reliably, use is safe, is suitable for Treat Bronchial Asthmas.

CN104352691A (application number: 201410749343.8, Jiang Chunhua) provides a kind of bronchial asthma for the treatment of Traditional Chinese herbal decoction, it is characterised in that it is made of the component of the material medicine of following weight proportion: it mustard seed 25g, processs radix scutellariae 9g, is white Pulp 9g, almond 9g, tuber of pinellia 9g, tussilago 10g, root bark of white mulberry 12g, radix scutellariae 9g, safflower 12g, Fructus Aurantii 12g, radix glycyrrhizae preparata 6g.

CN107823328A (application number: 201711035291.8, Jin Tanghai receive) roars according to Chinese traditional treatment infantile cough's property The understanding mechanism of asthma and a kind of endo-medicine for treating infantile cough's property asthma is provided, each component and its quality of the drug Part ratio is as follows: 30~120g of the root of purple-flowered peucedanum, 80~160g of Radix Angelicae Sinensis, 30~70g of Chinese gall, 30~100g of mustard seed, 30~120g of campanulaceae, party Join 150~300g, 150~350g of the root bark of Chinese wolf-berry, 60~150g of the root bark of white mulberry, 30~100g of donkey-hide gelatin, 100~160g of Poria cocos, Inula britannica chinensis 30~100g, 100~160g of clam shell, 50~120g of great burdock achene, 25~150g of radix scutellariae, 60~150g of dark plum, 25~70g of the coptis, 35~120g of dried orange peel, auxiliary material: superfine silica gel powder, microcrystalline cellulose and appropriate starch；It is believed that the invention provide drug be one very Good Chinese prescription.

CN108186832A (application number: 201810139225.3, Liuzhou women and children), which is disclosed, a kind of treats infantile cough's change The Chinese medicinal granule and preparation method thereof of anisotropic asthma.It is described treatment cough variant asthma in childhood: prednison comparing Chinese medicinal granule by Following raw material components are prepared: toast radix scutellariae, the root bark of white mulberry, radix scutellariae, ardisia japonica, mustard seed, aster, tussilago, perilla seed, pheretima, Campanulaceae, bombyx batryticatus, rhizoma pinellinae praeparata, semen armeniacae amarae, radix glycyrrhizae preparata, dried orange peel, cicada slough, lactose.It is believed that invention treatment infantile cough's variability is roared The Chinese medicinal granule of asthma according to traditional Chinese medical science prescription and preparation principle develop meticulously, according to Chinese medicine modern extraction technique extract effectively at Point, have the characteristics that with strong points, good effect, period are short, have no toxic side effect.

CN108653550A (application number: 201810749962.5, long hundred million sail in day) disclose a kind of numb a kind of reed mentioned in ancient books disappear cough drug and Preparation method and detection method.The fiber crops a kind of reed mentioned in ancient books cough drug that disappears is made of the flavour of a drug raw material of following parts by weight: sweet radix scutellariae 110~ 125g, 110~125g of the root bark of white mulberry, 225~240g of honeysuckle, 110~125g of radix scutellariae, 205~220g of Thesium chinese, gypsum 205~ 220g, 110~125g of fritillaria thunbergii, 110~125g of semen armeniacae amarae, 110~125g of perilla seed, 110~125g of tussilago, mustard seed 110 ~125g, 205~220g of pheretima, 75~90g of pinellia fry 110~125g of gingko benevolence, 75~90g of radix glycyrrhizae preparata.Invention provides Disappeared the method for cough active pharmaceutical ingredient content using hplc simultaneous determination fiber crops a kind of reed mentioned in ancient books, the present invention also provides numb a kind of reed mentioned in ancient books to disappear New application of the cough drug in preparation treatment Chronic Obstructive Pulmonary Disease.

CN104383182A (application number: 201410748990.7, Jiang Chunhua) discloses a kind of bronchial asthma for the treatment of Chinese medicine, it is characterised in that it is made of the component of the material medicine of following weight proportion: mustard seed 20-30g, toast radix scutellariae 6-9g, Gingko meat 6-9g, almond 6-9g, tuber of pinellia 6-9g, tussilago 9-12g, root bark of white mulberry 12-15g, radix scutellariae 9-12g, safflower 9-12g, trifoliate orange Shell 12-15g, radix glycyrrhizae preparata 6-9g.

In addition, there is document using Chinese medicine preparation made of the medicinal materials such as radix scutellariae, tabasheer, semen brassicae for treating related disease Disease, for example, CN104623359A (application number: 201510017375.3) discloses a kind of Chinese medicine for treating infantile pneumonia.By with The Chinese medicine of lower parts by weight is made: 0.9~1.5 gram of indigo naturalis, 9~15 grams of the root bark of Chinese wolf-berry, 3~9 grams of tabasheer, 5~9 grams of Caulis Perillae, Process 1.5~9 grams of radix scutellariae, 3~9 grams of semen brassicae, 3~9 grams of mustard seed, 3~9 grams of radix scutellariae, 3~10 grams of dried orange peel, 3~10 grams of the tuber of pinellia, water 15~30 grams of ox horn, 10~25 grams of fresh Radix Rehmanniae, 3~12 grams of uncaria, 6~15 grams of raw Fructus Aurantii, 3~6 grams of arisaema cum bile, it is black lead a cow 9~ 15 grams, 3~7.5 grams of borneol, 10~25 grams of Snakegourd Seed, 1.5~5 grams of the coptis, 6~12 grams of great burdock achene, 3~9 grams of polygoni cuspidati,radix, wild buckwheat 12~30 grams of wheat root, 1.5~9 grams of licorice, 12~25 grams of paris polyphylla.It is believed that simple, good absorption effect is prepared in the invention, Duration of efficacy is long, significant in efficacy, without side-effects, highly-safe, and production cost is low, is conducive to sufferer and mitigates symptom in time.

CN106039211A (application number: 201610504264.X) is related to a kind of relieving convulsion and calming the nerves particle and preparation method thereof, It is prepared by the raw material of following parts by weight: 5~15 parts of tabasheer, 5~15 parts of bulbus fritillariae cirrhosae, 6~15 parts of horse bezoar, antelope It is 3~9 parts of goat's horn, 5~15 parts of ambergris, 5~15 parts of bombyx batryticatus, 5~15 parts of rhizoma pinellinae praeparata, 3~9 parts of arisaema cum bile, 3~9 parts of centipede, complete 7~21 parts of scorpion, 5~15 parts of Rhizoma Gastrodiae, 3~15 parts of Radix Curcumae, 2~10 parts of alum, 3~15 parts of the coptis, 3~15 parts of magnetite, rheum officinale 5~ 20 parts, 5~15 parts of Exocarpium Citri Rubrum, 3~12 parts of cape jasmine, 3~9 parts of Medicated Leaven, 3~9 parts of agalloch eaglewood, green 3~12 parts of chlorite schist, Poria cocos 5~20 Part, 3~12 parts of Cortex Phellodendri, 3~9 parts of mustard seed, 3~12 parts of radix scutellariae, 3~9 parts of Radix Angelicae Sinensis, 5~15 parts of bezoar from monkeys, 2~6 parts of cow-bezoar, Moschus 2 ~6 parts, 5~20 parts of cornu bubali, 3~9 parts of amber and 3~9 parts of radix glycyrrhizae preparata.It is believed that the relieving convulsion particle significantly can cure or prevent Epilepsy clonus disease, has no toxic side effect, and has significant curative effect, and contained various traditional Chinese medicine ingredients mutually act synergistically, and not only has solidifying The effect of refreshing sleeping, while having effects that heat-clearing relieve heat, eliminating the phlegm resolving sputum and eliminating phlegm for resuscitation, be conducive to protect human health.

However, the Chinese prescription that existing document is recorded is complex in composition, the validity of some prescriptions also waits to investigate.Cause This, the method for having new is still expected in this field, and lung heat cough caused by a variety of causes is treated especially with the method for traditional Chinese medicine It coughs, the illnesss such as phlegm is contained, wheezed, phlegm Huang, red tongue with yellowish fur, especially expecting to provide one kind can be used in pediatric patient clearing lung and eliminating phlegm and stop The Chinese medicine composition of cloperastine asthma.

Summary of the invention

The purpose of the present invention is to provide a kind of simple Chinese traditional medicine compositions to treat cough with lung heat caused by a variety of causes, phlegm It contains, wheeze, the illnesss such as phlegm Huang, red tongue with yellowish fur, the present invention, which it is desirable to provide, can be used in pediatric patient clearing lung, eliminating phlegm, relieving cough & asthma Chinese medicine composition, the present invention it is desirable to provide it is a kind of treat cough with lung heat caused by a variety of causes, phlegm is contained, is wheezed, phlegm is yellow, tongue The method of the illnesss such as red yellow tongue fur, the present invention it is desirable to provide the method for preparing this kind of Chinese medicine composition.Unexpected hair Existing, the present invention is using relatively simple prescription can effectively treat cough with lung heat caused by a variety of causes, phlegm is contained, wheezed, phlegm The illnesss such as Huang, red tongue with yellowish fur are especially efficiently used for pediatric patient clearing lung, eliminating phlegm, relieving cough & asthma, and the present invention is based on such discoveries And it is accomplished.

For this purpose, first aspect present invention provides a kind of Chinese medicine composition, by including following Chinese medicine and optionally Pharmaceutic adjuvant be prepared: radix scutellariae, tabasheer, mustard seed.

Chinese medicine composition according to a first aspect of the present invention is prepared: radix scutellariae 1 by the Chinese medicine for including following ratio Parts by weight, 0.2~5 parts by weight of tabasheer, 0.05~2 parts by weight of mustard seed.

Chinese medicine composition according to a first aspect of the present invention is prepared: radix scutellariae 1 by the Chinese medicine for including following ratio Parts by weight, 0.25~2.5 parts by weight of tabasheer, 0.1~1 parts by weight of mustard seed.

Chinese medicine composition according to a first aspect of the present invention is prepared: radix scutellariae 1 by the Chinese medicine for including following ratio Parts by weight, 0.5~2 parts by weight of tabasheer, 0.1~0.5 parts by weight of mustard seed.

Chinese medicine composition according to a first aspect of the present invention is prepared: radix scutellariae 1 by the Chinese medicine for including following ratio Parts by weight, 1 parts by weight of tabasheer, 0.15 parts by weight of mustard seed.

Chinese medicine composition according to a first aspect of the present invention, wherein the radix scutellariae, SCUTELLARIAE RADIX, are lip shape The dry root of section plant radix scutellariae Scutellaria baicalensis Georgi.Spring, the excavation of two season of autumn, remove fibrous root and mud Sand hits rough bark after solarization, dries.Radix scutellariae is in cone, distortion, long 8~25cm, 1~3cm of diameter.Surface brown color is deep yellow Color has sparse excipuliform radicula trace, and top is rougher, has the vertical wrinkle of distortion or irregular reticulate pattern, lower part to have rift grain and thin Wrinkle.Matter is hard and crisp, frangibility, section yellow, center rufous；Old root center is in dry and decayed shape or hollow, burgundy or dark brown Color.Gas is micro-, bitter.Medicine materical crude slice scutellaria tablet is made in the following way: impurity removed, sets and is boiled in boiling water 10 minutes, is taken out, it is bored, Shave, it is dry；Or half an hour is steamed, it takes out, shave, dry (attention avoids being exposed to the sun)；For similar round or irregular shape thin slice, Exocuticle yellowish-brown or sepia, section yellowish-brown or yellow green, have radial texture.Medicine materical crude slice stir-baked RADIX SCUTELLARIAE with yellow rice wine is made in the following way At: scutellaria tablet is taken, processs method (four general rules 0213 of Chinese Pharmacopoeia version in 2015) fried dry according to wine；It is micro- shaped like scutellaria tablet, slightly focal spot There is wine aroma.Medicine materical crude slice stir-baked RADIX SCUTELLARIAE is made in the following way: taking scutellaria tablet with the micro- coke degree of being in surface is fried with slow fire, takes out, cool. Medicine materical crude slice charred Radix Scutellariae is made in the following way: taking scutellaria tablet to be fried with high heat to surface coke brown, marginal belt black is degree, but must be deposited Property, clear water is sprayed, is taken out, is dried.Radix scutellariae bitter, it is cold in nature；Return lung, gallbladder, spleen, large intestine, small intestinl channel；With heat-clearing and damp-drying drug, purging intense heat The effect of removing toxic substances, hemostasis, miscarriage prevention；For damp-warm syndrome, summer-heat and damp, chest tightness and emesis, damp and hot feeling of fullness, dysentery, jaundice, cough with lung heat, high fever Polydipsia, blood-head tells nosebleed, carbuncle sore tumefacting virus, threatened abortion.According to the present invention, the radix scutellariae can be radix scutellariae medicinal materials itself, can also It is medicine materical crude slice scutellaria tablet, stir-baked RADIX SCUTELLARIAE with yellow rice wine, stir-baked RADIX SCUTELLARIAE, charred Radix Scutellariae.Various radix scutellariaes and their various medicine materical crude slice can lead in the present invention With their chemical substance and physiological role substantially indistinction.It is used if not otherwise specified below in specific example Radix scutellariae is scutellaria tablet.

Chinese medicine composition according to a first aspect of the present invention, wherein the tabasheer, BAMBUSAE CONCRETIO SILICEA is gramineae plant bambusa texlitis Bambusa textilis McClure or schizostachyum chinense Rendle Schizostachyum The block after juice drying in the stalks such as chinense Rendle.Autumn, the harvesting of two season of winter.Tabasheer is irregular Block or particle, it is not of uniform size.Surface dusty blue, lark or canescence, the pure white color having is translucent, slightly gloss.Body is light, Matter is hard and crisp, easily broken, and hygroscopicity is strong.Gas is micro-, lightly seasoned.Tabasheer is sweet in flavor, cold in nature；Converge to heart and liver channels；With heat phlegm, cool The effect of heart is calmed the frightened；For pyreticosis coma, stroke and phlegm confusing heart, the hot frightened epilepsy of children's phlegm, twitch, the morbid night crying of babies.According to the present invention, the India Huang can be the tabasheer in various bamboo sources.The tabasheer in various sources in the present invention can be general, their chemical substance And physiological role substantially indistinction.Below in specific example, tabasheer used is that bambusa texlitis comes if not otherwise specified Source.

Chinese medicine composition according to a first aspect of the present invention, wherein the mustard seed, SINAPIS SEMEN, are Cruciferae The dry mature seed of plant mustard Sinapis alba L. or mustard Brassica juncea (L.) Czern.et Coss..The former It practises and claims " semen brassicae ", the latter, which practises, claims " SEMEN SINAPIS ALBAE ".Plant is tapped when fruit maturation at the beginning of autumn late summer, is dried, seed is laid, removes impurity elimination Matter.Semen brassicae is spherical in shape, 1.5~2.5mm of diameter.Surface canescence has subtle reticulate pattern to faint yellow, has dotted kind apparent Navel.Kind of thin skin and it is crisp, break the cotyledon for thering is white to fold in rear, there is oiliness.Gas is micro-, and acrid flavour is peppery.SEMEN SINAPIS ALBAE is smaller, and diameter 1~ 2mm.For surface yellow to brown color, minority is in reddish dark brown.Add water-soaked after grinding, then generates pungent strong special foul smell.Medicine materical crude slice Mustard seed is made in the following way: removing impurity, the used time smashs to pieces.Medicine materical crude slice fried mustard seed is made in the following way: taking net mustard seed, shines Method (four general rules 0213 of Chinese Pharmacopoeia version in 2015) are fried to fry to faint yellow to buff (stir-baked SINAPIS ALBA) or buff to palm fibre Brown (stir-bake to yellowish mustard seed), there is spicy gas；Used time smashs to pieces；For fried mustard seed shaped like mustard seed, surface is faint yellow to buff (stir-baked SINAPIS ALBA) Or buff occasionally has focal spot to sepia (stir-bake to yellowish mustard seed).There is spicy gas.It is mustard seed acrid flavour, warm-natured, it is distributed in lung channel；It slits with warm lung The effect of phlegm benefit gas, dissipating bind is removed obstruction in channels to relieve pain；It coughs for productive cough of trembling with fear, distending pain in the chest and hypochondrium, the stagnant channels and collaterals of phlegm, joint numbness, pain, phlegm wet stream Note, negative subcutaneous ulcer pyogenic infections.According to the present invention, the mustard seed can be the mustard seed in various sources, it can be semen brassicae can also be yellow Mustard seed.According to the present invention, the mustard seed can be mustard seed medicinal material itself, can also be medicine materical crude slice mustard seed or medicine materical crude slice fried mustard seed.Respectively The mustard seed and their various medicine materical crude slice in kind source in the present invention can be general, their chemical substance and physiological role Substantially indistinction.Below in specific example, mustard seed used is semen brassicae if not otherwise specified.

Chinese medicine composition according to a first aspect of the present invention, wherein the radix scutellariae is in the form of medicinal powder or is It in the form of its extract or is to extract extract obtained form together with other one or more medicinal materials with it to be added to In the composition.

Chinese medicine composition according to a first aspect of the present invention, wherein the tabasheer be in the form of medicinal powder or It is in the form of its extract or is to extract extract obtained form addition together with other one or more medicinal materials with it Into the composition.

Chinese medicine composition according to a first aspect of the present invention, wherein the mustard seed is in the form of medicinal powder or is It in the form of its extract or is to extract extract obtained form together with other one or more medicinal materials with it to be added to In the composition.

Chinese medicine composition according to a first aspect of the present invention, wherein the medicinal powder is at least can be by 80 meshes Powder, or can at least lead to the powder sieved with 100 mesh sieve, or can at least pass through the powder of 120 meshes.Many institute's weeks Know, medicinal material can for example, by but be not limited to crush, grinding and combinations thereof etc. modes be processed into powder.

Chinese medicine composition according to a first aspect of the present invention, wherein the extract, the whether extract of single medicinal material Still a variety of medicinal materials extract extract obtained together, and the solvent of extraction can be selected from: water, ethyl alcohol, ethanol water such as concentration For 10~90% ethyl alcohol.

Chinese medicine composition according to a first aspect of the present invention, wherein the extraction can be any well known to pharmaceutical field mention Mode or combinations thereof is taken, such as, but not limited to: dipping, decoction, diacolation etc. preferably remove solvent after extraction.

Chinese medicine composition according to a first aspect of the present invention, wherein the powder can be optionally auxiliary with liquid or semisolid Material combination, such as combine with water, ethyl alcohol such as yellow rice wine, acetic acid such as vinegar and its aqueous solution etc. and to form paste and be for external application.

Chinese medicine composition according to a first aspect of the present invention is external preparation.In one embodiment, the external application system Agent is the forms such as patch, ointment, cream.In one embodiment, the external preparation be make the powder of the medicinal material with Liquid or semisolid auxiliary material are tuned into paste (such as the weight ratio of medicinal powder and Auxiliary Liquid Material is 1~5:1).Made with this paste-like preparation With smearing or stick on the skin, and then play its drug effect.

Chinese medicine composition according to a first aspect of the present invention is oral preparation.In one embodiment, the oral system Agent is in forms such as granule, tablet, capsule, oral solution, electuary, pill, sprays.These dosage forms are convenient to To administration for oral use.

Chinese medicine composition according to a first aspect of the present invention is external preparation or oral preparation, is according to including such as Under step be prepared: make each pulverizing medicinal materials at fine powder, or make each medicinal material individually or any combination is extracted Object, then external preparation or oral preparation is prepared with preparation process in gained fine powder or extract, optionally add in preparation Add pharmaceutic adjuvant.

Further, second aspect of the present invention provides the method for preparing Chinese medicine composition, is external preparation or mouth Formulation shines and includes the following steps, namely to be prepared: making each pulverizing medicinal materials of radix scutellariae, tabasheer, mustard seed at fine powder, or Make each medicinal material individually or any combination is extracted to obtain extract, then gained fine powder or extract are prepared outside with preparation process With preparation or oral preparation, pharmaceutic adjuvant is optionally added in preparation.

Method according to a second aspect of the present invention, the ratio of the Chinese medicine are as follows: 1 parts by weight of radix scutellariae, tabasheer 0.2~5 Parts by weight, 0.05~2 parts by weight of mustard seed.

Method according to a second aspect of the present invention, the ratio of the Chinese medicine are as follows: 1 parts by weight of radix scutellariae, tabasheer 0.25~ 2.5 parts by weight, 0.1~1 parts by weight of mustard seed.

Method according to a second aspect of the present invention, the ratio of the Chinese medicine are as follows: 1 parts by weight of radix scutellariae, tabasheer 0.5~2 Parts by weight, 0.1~0.5 parts by weight of mustard seed.

Method according to a second aspect of the present invention, the ratio of the Chinese medicine are as follows: 1 parts by weight of radix scutellariae, 1 weight of tabasheer Part, 0.15 parts by weight of mustard seed.

Method according to a second aspect of the present invention, wherein the radix scutellariae, SCUTELLARIAE RADIX, are Lamiaceae plant The dry root of radix scutellariae Scutellaria baicalensis Georgi.Spring, the excavation of two season of autumn, fibrous root and silt are removed, after solarization Rough bark is hit, is dried.Radix scutellariae is in cone, distortion, long 8~25cm, 1~3cm of diameter.Surface brown color or buff have dilute Thin excipuliform radicula trace, top is rougher, has the vertical wrinkle of distortion or irregular reticulate pattern, lower part to have rift grain and fine wrinkle.Matter It is hard and crisp, frangibility, section yellow, center rufous；Old root center is in dry and decayed shape or hollow, burgundy or brownish black.Gas is micro-, Bitter.Medicine materical crude slice scutellaria tablet is made in the following way: removing impurity, sets and boil in boiling water 10 minutes, take out, bored, shave, does It is dry；Or half an hour is steamed, it takes out, shave, dry (attention avoids being exposed to the sun)；For similar round or irregular shape thin slice, appearance Beijing opera Brown or sepia, section yellowish-brown or yellow green, have radial texture.Medicine materical crude slice stir-baked RADIX SCUTELLARIAE with yellow rice wine is made in the following way: taking Huang A kind of reed mentioned in ancient books piece processs method (four general rules 0213 of Chinese Pharmacopoeia version in 2015) fried dry according to wine；It is micro- to have aroma shaped like scutellaria tablet, slightly focal spot Gas.Radix scutellariae bitter, it is cold in nature；Return lung, gallbladder, spleen, large intestine, small intestinl channel；With heat-clearing and damp-drying drug, purging fire for removing toxin, hemostasis, the function of miscarriage prevention Effect；For damp-warm syndrome, summer-heat and damp, chest tightness and emesis, damp and hot feeling of fullness, dysentery, jaundice, cough with lung heat, hyperpyrexia polydipsia, blood-head tells nosebleed, carbuncle swells Sore, threatened abortion.According to the present invention, the radix scutellariae can be radix scutellariae medicinal materials itself, can also be medicine materical crude slice scutellaria tablet or wine Radix scutellariae.Various radix scutellariaes and their various medicine materical crude slice in the present invention can be general, their chemical substance and physiology is made With substantially indistinction.

Method according to a second aspect of the present invention, wherein the tabasheer, BAMBUSAE CONCRETIO SILICEA, are Gramineae plant bambusa texlitis Bambusa textilis McClure or schizostachyum chinense Rendle Schizostachyum chinense The block after juice drying in the stalks such as Rendle.Autumn, the harvesting of two season of winter.Tabasheer be irregular tile or particle, It is not of uniform size.Surface dusty blue, lark or canescence, the pure white color having is translucent, slightly gloss.Body is light, and matter is hard and crisp, Easily broken, hygroscopicity is strong.Gas is micro-, lightly seasoned.Tabasheer is sweet in flavor, cold in nature；Converge to heart and liver channels；Function with heat phlegm, cool heart arresting convulsion Effect；For pyreticosis coma, stroke and phlegm confusing heart, the hot frightened epilepsy of children's phlegm, twitch, the morbid night crying of babies.According to the present invention, the tabasheer can be respectively The tabasheer in kind bamboo source.The tabasheer in various sources in the present invention can be general, their chemical substance and physiology Effect substantially indistinction.

Method according to a second aspect of the present invention, wherein the mustard seed, SINAPIS SEMEN, are crucifer mustard The dry mature seed of Sinapis alba L. or mustard Brassica juncea (L.) Czern.et Coss..The former practises title " semen brassicae ", the latter, which practises, claims " SEMEN SINAPIS ALBAE ".Plant is tapped when fruit maturation at the beginning of autumn late summer, is dried, seed is laid, removes impurity. Semen brassicae is spherical in shape, 1.5~2.5mm of diameter.Surface canescence has subtle reticulate pattern to faint yellow, there is apparent dotted hilum. Kind of thin skin and it is crisp, break the cotyledon for thering is white to fold in rear, there is oiliness.Gas is micro-, and acrid flavour is peppery.SEMEN SINAPIS ALBAE is smaller, 1~2mm of diameter. For surface yellow to brown color, minority is in reddish dark brown.Add water-soaked after grinding, then generates pungent strong special foul smell.Medicine materical crude slice mustard seed It is made in the following way: removing impurity, the used time smashs to pieces.Medicine materical crude slice fried mustard seed is made in the following way: net mustard seed is taken, according to frying Method (four general rules 0213 of Chinese Pharmacopoeia version in 2015) is fried to faint yellow to buff (stir-baked SINAPIS ALBA) or buff to sepia (stir-bake to yellowish mustard seed), there is spicy gas；Used time smashs to pieces；For fried mustard seed shaped like mustard seed, surface is faint yellow to buff (stir-baked SINAPIS ALBA) or deep Yellow occasionally has focal spot to sepia (stir-bake to yellowish mustard seed).There is spicy gas.It is mustard seed acrid flavour, warm-natured, it is distributed in lung channel；With warm lung slit phlegm benefit The effect of gas, dissipating bind is removed obstruction in channels to relieve pain；Cough for productive cough of trembling with fear, distending pain in the chest and hypochondrium, the stagnant channels and collaterals of phlegm, joint numbness, pain, phlegm-damp oozing in the body, Negative subcutaneous ulcer pyogenic infections.According to the present invention, the mustard seed can be the mustard seed in various sources, it can be semen brassicae can also be yellow mustard Son.According to the present invention, the mustard seed can be mustard seed medicinal material itself, can also be medicine materical crude slice mustard seed or medicine materical crude slice fried mustard seed.It is various The mustard seed in source and their various medicine materical crude slice in the present invention can be general, their chemical substance and physiological role sheet Indistinction in matter.

Method according to a second aspect of the present invention, wherein the radix scutellariae is in the form of medicinal powder or mentioned with it It takes the form of object or is to extract extract obtained form together with other one or more medicinal materials with it to be added to described group It closes in object.

Method according to a second aspect of the present invention, wherein the tabasheer is in the form of medicinal powder or is with it The form of extract or be extracted together with other one or more medicinal materials with it extract obtained form be added to it is described In composition.

Method according to a second aspect of the present invention, wherein the mustard seed is in the form of medicinal powder or mentioned with it It takes the form of object or is to extract extract obtained form together with other one or more medicinal materials with it to be added to described group It closes in object.

Method according to a second aspect of the present invention, wherein the medicinal powder be at least can by the powder of 80 meshes, It either can at least lead to the powder sieved with 100 mesh sieve, or can at least pass through the powder of 120 meshes.It is well known that medicinal material Can for example, by but be not limited to crush, grinding and combinations thereof etc. modes be processed into powder.

Method according to a second aspect of the present invention, wherein the extract, the whether extract of single medicinal material or more Kind of medicinal material extracts extract obtained together, and the solvent of extraction can be selected from: water, ethyl alcohol, ethanol water such as concentration is 10~ 90% ethyl alcohol.

Method according to a second aspect of the present invention, wherein the extraction can be any extracting mode well known to pharmaceutical field Or combinations thereof, such as, but not limited to: dipping, decoction, diacolation etc. preferably remove solvent after extraction.

Method according to a second aspect of the present invention, wherein the powder can optionally with liquid or semisolid auxiliary material group It closes, such as combines with water, ethyl alcohol such as yellow rice wine, acetic acid such as vinegar and its aqueous solution etc. and to form paste and be for external application.

Method according to a second aspect of the present invention, wherein the Chinese medicine composition is external preparation.In an embodiment In, which is the forms such as patch, ointment, cream.In one embodiment, which is to make the medicine The powder of material and liquid or semisolid auxiliary material are tuned into paste (such as the weight ratio of medicinal powder and Auxiliary Liquid Material is 1~5:1).It is pasted with this Shape preparation uses, and smears or sticks on the skin, and then plays its drug effect.

Method according to a second aspect of the present invention, wherein the Chinese medicine composition is oral preparation.In an embodiment In, which is in forms such as granule, tablet, capsule, oral solution, electuary, pill, sprays.These preparation shapes Formula is convenient to administration for oral use.

Method according to a second aspect of the present invention, wherein the Chinese medicine composition be external preparation or oral preparation, According to including the following steps, namely to be prepared: making each pulverizing medicinal materials at fine powder, or make each medicinal material individually or any combination mentions Extract is obtained, then external preparation or oral preparation, preparation is prepared with preparation process in gained fine powder or extract In optionally add pharmaceutic adjuvant.

Further, third aspect present invention provides the combination including following Chinese medicine and stops in preparation for clearing lung and eliminating phlegm Purposes in the drug of cloperastine asthma, or preparation for treating cough with lung heat, phlegm is contained, wheeze, the drug of phlegm Huang, red tongue with yellowish fur In purposes: radix scutellariae, tabasheer, mustard seed.

Purposes according to a third aspect of the present invention, the drug is for pediatric patient clearing lung, eliminating phlegm, relieving cough & asthma or is used for Pediatric patient cough with lung heat, phlegm contain, wheeze, phlegm is yellow, red tongue with yellowish fur.

Purposes according to a third aspect of the present invention, wherein the drug is by including that the Chinese medicine of following ratio is prepared: 1 parts by weight of radix scutellariae, 0.2~5 parts by weight of tabasheer, 0.05~2 parts by weight of mustard seed.

Purposes according to a third aspect of the present invention, wherein the drug is by including that the Chinese medicine of following ratio is prepared: 1 parts by weight of radix scutellariae, 0.25~2.5 parts by weight of tabasheer, 0.1~1 parts by weight of mustard seed.

Purposes according to a third aspect of the present invention, wherein the drug is by including that the Chinese medicine of following ratio is prepared: 1 parts by weight of radix scutellariae, 0.5~2 parts by weight of tabasheer, 0.1~0.5 parts by weight of mustard seed.

Purposes according to a third aspect of the present invention, wherein the drug is by including that the Chinese medicine of following ratio is prepared: 1 parts by weight of radix scutellariae, 1 parts by weight of tabasheer, 0.15 parts by weight of mustard seed.

Purposes according to a third aspect of the present invention, wherein the radix scutellariae, SCUTELLARIAE RADIX, are Lamiaceae plant The dry root of radix scutellariae Scutellaria baicalensis Georgi.Spring, the excavation of two season of autumn, fibrous root and silt are removed, after solarization Rough bark is hit, is dried.Radix scutellariae is in cone, distortion, long 8~25cm, 1~3cm of diameter.Surface brown color or buff have dilute Thin excipuliform radicula trace, top is rougher, has the vertical wrinkle of distortion or irregular reticulate pattern, lower part to have rift grain and fine wrinkle.Matter It is hard and crisp, frangibility, section yellow, center rufous；Old root center is in dry and decayed shape or hollow, burgundy or brownish black.Gas is micro-, Bitter.Medicine materical crude slice scutellaria tablet is made in the following way: removing impurity, sets and boil in boiling water 10 minutes, take out, bored, shave, does It is dry；Or half an hour is steamed, it takes out, shave, dry (attention avoids being exposed to the sun)；For similar round or irregular shape thin slice, appearance Beijing opera Brown or sepia, section yellowish-brown or yellow green, have radial texture.Medicine materical crude slice stir-baked RADIX SCUTELLARIAE with yellow rice wine is made in the following way: taking Huang A kind of reed mentioned in ancient books piece processs method (four general rules 0213 of Chinese Pharmacopoeia version in 2015) fried dry according to wine；It is micro- to have aroma shaped like scutellaria tablet, slightly focal spot Gas.Radix scutellariae bitter, it is cold in nature；Return lung, gallbladder, spleen, large intestine, small intestinl channel；With heat-clearing and damp-drying drug, purging fire for removing toxin, hemostasis, the function of miscarriage prevention Effect；For damp-warm syndrome, summer-heat and damp, chest tightness and emesis, damp and hot feeling of fullness, dysentery, jaundice, cough with lung heat, hyperpyrexia polydipsia, blood-head tells nosebleed, carbuncle swells Sore, threatened abortion.According to the present invention, the radix scutellariae can be radix scutellariae medicinal materials itself, can also be medicine materical crude slice scutellaria tablet or wine Radix scutellariae.Various radix scutellariaes and their various medicine materical crude slice in the present invention can be general, their chemical substance and physiology is made With substantially indistinction.

Purposes according to a third aspect of the present invention, wherein the tabasheer, BAMBUSAE CONCRETIO SILICEA, are Gramineae plant bambusa texlitis Bambusa textilis McClure or schizostachyum chinense Rendle Schizostachyum chinense The block after juice drying in the stalks such as Rendle.Autumn, the harvesting of two season of winter.Tabasheer be irregular tile or particle, It is not of uniform size.Surface dusty blue, lark or canescence, the pure white color having is translucent, slightly gloss.Body is light, and matter is hard and crisp, Easily broken, hygroscopicity is strong.Gas is micro-, lightly seasoned.Tabasheer is sweet in flavor, cold in nature；Converge to heart and liver channels；Function with heat phlegm, cool heart arresting convulsion Effect；For pyreticosis coma, stroke and phlegm confusing heart, the hot frightened epilepsy of children's phlegm, twitch, the morbid night crying of babies.According to the present invention, the tabasheer can be respectively The tabasheer in kind bamboo source.The tabasheer in various sources in the present invention can be general, their chemical substance and physiology Effect substantially indistinction.

Purposes according to a third aspect of the present invention, wherein the mustard seed, SINAPIS SEMEN, are crucifer mustard The dry mature seed of Sinapis alba L. or mustard Brassica juncea (L.) Czern.et Coss..The former practises title " semen brassicae ", the latter, which practises, claims " SEMEN SINAPIS ALBAE ".Plant is tapped when fruit maturation at the beginning of autumn late summer, is dried, seed is laid, removes impurity. Semen brassicae is spherical in shape, 1.5~2.5mm of diameter.Surface canescence has subtle reticulate pattern to faint yellow, there is apparent dotted hilum. Kind of thin skin and it is crisp, break the cotyledon for thering is white to fold in rear, there is oiliness.Gas is micro-, and acrid flavour is peppery.SEMEN SINAPIS ALBAE is smaller, 1~2mm of diameter. For surface yellow to brown color, minority is in reddish dark brown.Add water-soaked after grinding, then generates pungent strong special foul smell.Medicine materical crude slice mustard seed It is made in the following way: removing impurity, the used time smashs to pieces.Medicine materical crude slice fried mustard seed is made in the following way: net mustard seed is taken, according to frying Method (four general rules 0213 of Chinese Pharmacopoeia version in 2015) is fried to faint yellow to buff (stir-baked SINAPIS ALBA) or buff to sepia (stir-bake to yellowish mustard seed), there is spicy gas；Used time smashs to pieces；For fried mustard seed shaped like mustard seed, surface is faint yellow to buff (stir-baked SINAPIS ALBA) or deep Yellow occasionally has focal spot to sepia (stir-bake to yellowish mustard seed).There is spicy gas.It is mustard seed acrid flavour, warm-natured, it is distributed in lung channel；With warm lung slit phlegm benefit The effect of gas, dissipating bind is removed obstruction in channels to relieve pain；Cough for productive cough of trembling with fear, distending pain in the chest and hypochondrium, the stagnant channels and collaterals of phlegm, joint numbness, pain, phlegm-damp oozing in the body, Negative subcutaneous ulcer pyogenic infections.According to the present invention, the mustard seed can be the mustard seed in various sources, it can be semen brassicae can also be yellow mustard Son.According to the present invention, the mustard seed can be mustard seed medicinal material itself, can also be medicine materical crude slice mustard seed or medicine materical crude slice fried mustard seed.It is various The mustard seed in source and their various medicine materical crude slice in the present invention can be general, their chemical substance and physiological role sheet Indistinction in matter.

Purposes according to a third aspect of the present invention, wherein the radix scutellariae is in the form of medicinal powder or mentioned with it It takes the form of object or is to extract extract obtained form together with other one or more medicinal materials with it to be added to described group It closes in object.

Purposes according to a third aspect of the present invention, wherein the tabasheer is in the form of medicinal powder or is with it The form of extract or be extracted together with other one or more medicinal materials with it extract obtained form be added to it is described In composition.

Purposes according to a third aspect of the present invention, wherein the mustard seed is in the form of medicinal powder or mentioned with it It takes the form of object or is to extract extract obtained form together with other one or more medicinal materials with it to be added to described group It closes in object.

Purposes according to a third aspect of the present invention, wherein the medicinal powder be at least can by the powder of 80 meshes, It either can at least lead to the powder sieved with 100 mesh sieve, or can at least pass through the powder of 120 meshes.It is well known that medicinal material Can for example, by but be not limited to crush, grinding and combinations thereof etc. modes be processed into powder.

Purposes according to a third aspect of the present invention, wherein the extract, the whether extract of single medicinal material or more Kind of medicinal material extracts extract obtained together, and the solvent of extraction can be selected from: water, ethyl alcohol, ethanol water such as concentration is 10~ 90% ethyl alcohol.

Purposes according to a third aspect of the present invention, wherein the extraction can be any extracting mode well known to pharmaceutical field Or combinations thereof, such as, but not limited to: dipping, decoction, diacolation etc. preferably remove solvent after extraction.

Purposes according to a third aspect of the present invention, wherein the powder can optionally with liquid or semisolid auxiliary material group It closes, such as combines with water, ethyl alcohol such as yellow rice wine, acetic acid such as vinegar and its aqueous solution etc. and to form paste and be for external application.

Purposes according to a third aspect of the present invention, wherein the drug is external preparation.In one embodiment, this is outer It is the forms such as patch, ointment, cream with preparation.In one embodiment, which is the powder for making the medicinal material End and liquid or semisolid auxiliary material are tuned into paste (such as the weight ratio of medicinal powder and Auxiliary Liquid Material is 1~5:1).With this paste-like preparation It uses, smears or stick on the skin, and then play its drug effect.

Purposes according to a third aspect of the present invention, wherein the drug is oral preparation.In one embodiment, the mouth Formulation is in forms such as granule, tablet, capsule, oral solution, electuary, pill, sprays.These dosage forms can be square Just administration for oral use is given.

Purposes according to a third aspect of the present invention, wherein the drug is external preparation or oral preparation, it is according to packet Include what following step was prepared: making each pulverizing medicinal materials at fine powder, or make each medicinal material individually or any combination extract to obtain Extract, then external preparation or oral preparation is prepared with preparation process in gained fine powder or extract, in preparation optionally Add pharmaceutic adjuvant in ground.

Further, fourth aspect present invention provides any one composition to the first aspect of the present invention and carries out quality The method of detection comprising assay, packet are carried out to the Sinapine Thiocyanate in composition using high performance liquid chromatography Include following operation:

According to the rule of Section of 0512 contained high performance liquid chromatography of four general rules of the version Pharmacopoeia of the People's Republic of China in 2015 Model measurement；

Chromatographic condition and system suitability: using octadecylsilane chemically bonded silica as filler；With acetonitrile- 0.08mol/L potassium dihydrogen phosphate is using the mixed liquor of volume ratio 10:90 as mobile phase；Detection wavelength is 326mn；Number of theoretical plate It is calculated by sinapine peak and is not less than 3000；

The preparation of reference substance solution: taking Sinapine Thiocyanate reference substance appropriate, accurately weighed, and mobile phase is added to be made often Solution of the 1ml containing 0.2mg to get；

The preparation of test solution: taking the test sample for being equivalent to mustard seed medicinal material 1g, accurately weighed, sets in stuffed conical flask, Methanol 50ml is added, with power 250W, frequency 20kHz ultrasonic treatment 20 minutes, filtration, filter residue again with methanol extracted three with method Secondary, filtrate merges；Solvent is recovered under reduced pressure to doing, residue adds flowing phased soln, is transferred in 50ml measuring bottle, is diluted to mobile phase Scale shakes up, filtration, take subsequent filtrate to get；

Measuring method: accurate absorption reference substance solution and each 10 μ l of test solution respectively, injection liquid chromatograph, measurement, By external standard method with Sinapine Thiocyanate, that is, C16H24NO5SCN content in calculated by peak area test sample.

Method according to a fourth aspect of the present invention, wherein the preparation of test solution is taken under type such as and is carried out: taking picture It is accurately weighed when in the test sample of mustard seed medicinal material 1g, it sets in stuffed conical flask, methanol 50ml, calcium chloride 100mg, ice second is added 20 μ l of acid, with power 250W, frequency 20kHz ultrasonic treatment 20 minutes, filtration, filter residue again with methanol was extracted three times with method, filtrate Merge；Solvent is recovered under reduced pressure to doing, residue adds flowing phased soln, is transferred in 50ml measuring bottle, is diluted to scale with mobile phase, shakes It is even, filtration, take subsequent filtrate to get.It has been found that, it may be possible to since there are radix scutellariaes in prescription, use conventional method measurement group When closing the Sinapine Thiocyanate in object, content is significantly lower than theoretical amount；It was unexpectedly determined that by prepare test sample molten When liquid, adding a small amount of calcium chloride and glacial acetic acid can be efficiently against the above problem.

In the above-mentioned preparation method of the invention the step of, although the specific steps of its description are in certain details or language In description with the preparation example of following detailed description part described in step different from, however, those skilled in the art The detailed disclosure of member's full text according to the present invention can summarize approach described above step completely.

Any embodiment in either present invention face can be combined with other embodiments, as long as they are not It will appear contradiction.In addition, any technical characteristic can be adapted for other realities in any embodiment of either side of the present invention The technical characteristic in scheme is applied, as long as they are not in contradiction.The invention will be further described below.

All documents recited in the present invention, their full content are incorporated herein by reference, and if these are literary When offering expressed meaning and the inconsistent present invention, it is subject to statement of the invention.In addition, the various terms that use of the present invention and Phrase has that well known to a person skilled in the art general senses, nonetheless, the present invention remain desirable at this to these terms and Phrase is described in more detail and explains, the term and phrase referred to is if any inconsistent with common art-recognized meanings, with institute's table of the present invention Subject to the meaning stated.

Radix scutellariae is the herbal medicine that " Chinese Pharmacopoeia " is included, radix scutellariae real name " a kind of reed mentioned in ancient books ", is to have popular name " yellow because careless color is yellow for a kind of reed mentioned in ancient books grass A kind of reed mentioned in ancient books "." a kind of reed mentioned in ancient books " word literal sense is " Folium Callicarpae Formosanae ".Alias Radix Scutellariae, tsuchikane tea root.Radix scutellariae is used as medicine with root.Originate in Hebei, Liaoning, Shaanxi, The ground such as Shandong, the Inner Mongol, Heilungkiang.It is radix scutellariae bitter, cold in nature；Distributed in lung channel, the heart channel of Hang-Shaoyin, Liver Channel, gallbladder channel, large intestine channel.Radix scutellariae, which has, to rush down The effect of fiery removing toxic substances, hemostasis, heat-clearing and damp-drying drug, miscarriage prevention；For damp-warm syndrome, jaundice, cough with lung heat, hyperpyrexia polydipsia, blood-head tells nosebleed, heat temperature It is chest tightness and emesis, damp and hot feeling of fullness, dysentery, carbuncle sore tumefacting virus, threatened abortion, high fever and coma due to pyreticosis, irascibility headache, red eye, swell pain, damp and hot Jaundice, dysentery, heat gonorrhea, hematemesis blood, metrorrhagia and metrostaxis, fetal movement with fever, carbuncle swells furunculosis.

The pharmacological action of radix scutellariae mainly has following aspect.1, antibacterial action: 100% concentration of radix scutellariae decoction, flat band method test, To shigella dysenteriae, typhoid bacillus, paratyphosum Bacterium, comma bacillus, Escherichia coli, proteus, Pseudomonas aeruginosa, staphylococcus, Hemolytic vessel used to hold grain at the imperial sacrifice coccus (a, B), Diplococcus pneumopniae, corynebacterium diphtheriae etc. have inhibiting effect.It is dense that radix scutellariae decoction test tube method tests 1:1280 Degree can inhibit typhoid bacillus, hemolytic streptococcus a, 1:640 concentration inhibits hemolytic streptococcus B, Diplococcus pneumopniae and Fu Shi dysentery Shigella, bacillus tuberculosis typus humanus's H37,1:320 concentration can inhibit comma bacillus, Shigella shigae；Sonnei when 1:80 concentration Shigella dysenteriae has inhibiting effect.Radix scutellariae alcohol immersion liquid 0.5g/ml or 0.05g/ml, with agar slant culture, medical fluid and culture medium 1:1 Mixing, has inhibiting effect to Pseudomonas aeruginosa.Radix scutellariae alcohol immersion liquid 2g/ml concentration, Plating test to Escherichia coli, tangerine straw bacterium, Staphylococcus aureus has inhibiting effect.Radix scutellariae decoction, alcohol extracting agent 1g/ml concentration, to meningococcus when being tested with flat band method There is suppression effect.2, antifungic action: radix scutellariae decocting liquid, test tube slant method test 4% concentration and inhibit dog denticle born of the same parents bacterium and violet hair Tinea bacterium, 8% concentration inhibit oidium schoenleinii, and 10% concentration inhibits the mutation of oidium schoenleinii Mongolia, and 15% concentration inhibits altogether Disposition trichophyta and rust trichophyta.Radix scutellariae flooding agent 1:3 concentration in vitro to trichophyton, Trichophyton concentricum, Oidium schoenleinii, Microsporon audouini, wool sample microsporum, epidermophyton rubrum, K, W, family name Epidermophyton, Star nocardia etc. has different degrees of bacteriostasis.3, antivirus action: radix scutellariae decoction 25-100% concentration, in vitro test pair Hepatitis B virus DNA is replicated with inhibiting effect.4, anti-inflammatory antiallergic action: 70% ethanol extract 200,500mg/kg of radix scutellariae Stomach-filling baicalein, baicalin, wogonin 50,100mg/kg stomach-filling can inhibit the exudation of mouse peritoneal caused by acetic acid and increase, right Rat is swollen enough caused by 48/80 (a kind of compound name, Sigma production) also inhibiting effect, 70% ethanol extract of radix scutellariae 500mg/kg stomach-filling, baicalein, baicalin and wogonin 100mg/kg stomach-filling have inhibiting effect to rat assist agent arthritis. Root of large-flowered skullcap water extract 100,200mg/kg stomach-filling have inhibiting effect to models of passive skin irritability of rats reaction (PCA), but draw to chloropicrin Mouse contact dermatitis (ear swelling) risen has no significant effect.Radix scutellariae inhibit passive cutaneous anaphylaxis (PCA) effective component be Baicalin and baicalein.Effectively to experimental asthma, baicalin 10-4g/ml concentration can inhibit guinea pig trachea for baicalin, baicalein Schultz-Dale reaction 38%, and have antihistamine, cholinolytic and the effect of papaverine sample.Baicalein is not soluble in water, two water Soluble derivatives baicalein -6- disodium hydrogen phosphate (BPS) and baicalein -6- disodium sulfate (BSS) 5mg/kg before antigen is attacked are quiet Arteries and veins injection, has inhibiting effect to P of Rats CA；10mg/kg intravenous injection is attacked first 10 minutes in antigen and is administered, to cavy anaphylaxis Asthma has inhibiting effect；BPS and BSS has isolated guinea pig intestinal tube and tracheae chultz-Dale reaction in 10-4g/ml concentration Inhibiting effect；BPS and BSS5mg/kg intravenous injection, has inhibiting effect to rat Reversed cutaneous anaphylaxis (RCA)；5-10mg/ Kg intravenous injection also inhibits Forssman cutaneous vasculitis, but has no significant effect to Arthus reaction.On baicalein and BPS structure It is similar with antiallergic disodium chromoglycate (DSCG), but DSCG is double-colored ketone, and baicalein and BPS are monochromatic ketone, DSCG The allergic reaction antibody-mediated to reagin (Reagin) has a high degree of specificity, and the mistake antibody-mediated to non-reagin (IgG) There are few the allergic reactions that effect, PBS then not only inhibit reaginic antibody to mediate, such as PBS10-6-10-4g/ml to inhibit anti-for quick reaction The release of monkey lung medium caused by original, PBS 200mg/kg intraperitoneal injection inhibit allotransplantation in rats PCA, 10-4g/ml to inhibit reagin anti- The rat hypertrophy cell degranulation etc. that body mediates, the reaction for also inhibiting non-reaginic antibody to mediate, as BPS10-4g/ml can inhibit People's lung caused by egg albumen sensitization Guinea pig lung dissolution medium and inhibition anti-IgE antibodies discharges Anaphylactic mediator, it is believed that DSCG molecule In two chromones are internuclear certain distance, can be combined in reaginic antibody functional location, and be not suitable for non-reaginic antibody, But the baicalein or BPS of monochromatic ketone can two molecules be incorporated on both antibody.Baicalein is to rat platelet peanut four Olefin(e) acid metabolism cyclooxygenase and lipoxygenase have inhibiting effect, more selective to lipoxygenase, and IC50 is 0.12 μm, and to ring The IC50 of oxygen is 6917 times of lipoxygenase.Baicalein can inhibit arachidonic acid metabolic to rat leukocyte, inhibit lipoxygenase generation Thank to the life of product 5- hydroxyeicosatetraenoic acid (5-HETE) and epoxidase metabolite 12- hydroxy heptadecatrienoic acid (HHT) At IC50 is respectively 7.13 ± 0.767 μm and 5.53 ± 16.9 μm；Baicalin also inhibits leucocyte 5-HETE formation, but The formation of HHT is not inhibited；Wogonin inhibits the formation of HHT, and IC50 is 14.6 ± 3.51 μm of ingredients of Scutellaria to compound 48/80 Rat peritoneal mast cells are stimulated to discharge the inhibiting effect of histamine, the IC50 of baicalein is 52.1 μm, and baicalin IC50 is > 200 μm, the IC50 of wogonin is 40.0 μm, and the IC50 of wogonin -7-O-D glucuronic acid is 140.0mol/l, (25) 2', The IC50 of tetra- hydroxyl flavanones of 5,6', 7- is 17.7 μm, and the IC50 of (2R, 3R) -2', 3,5,6', 7- pentahydroxy- flavanones is 15.5 μm, The IC50 of 200 μm of the IC50 > of Chrysin (Chrysin), neobaicalein II (Skullcapflavon II) are 15.0 μm.Radix scutellariae Glucoside 10,100mg/ml, generating PGE2 and TXA2 to the rat peritoneal macrophages of calcium ion carrier A 23187 induction has obviously Inhibiting effect.5, the effect of Central nervous system: radix scutellariae decoction 4g/kg intraperitoneal injection, it can to mouse defensive conditionedreflex Keep positive Reflection delay long, and radix scutellariae, which can reinforce cortex process of inhibition, to be illustrated without influence on unconditioned reflex and differentiation.Radix scutellariae is decocted Agent 2g/kg has refrigeration function to typhoid fever polyvalent vaccine pyrogenicity rabbit.But also have been reported that radix scutellariae water decoction or Liquor infusion 5-9g/ only Stomach-filling or 2g/ im not can prove that radix scutellariae has refrigeration function to antityphoid vaccine pyrogenicity rabbit.6, to cardiovascular effect: yellow A kind of reed mentioned in ancient books alcohol extract 1g/kg intravenous injection, can be such that anaesthetized dog blood pressure declines.Radix scutellariae decoction 0.06g/kg intravenous injection, there is anesthetized dog Obvious antihypertensive effect.Baicalein 20mg/kg intravenous injection, can make anaesthetized dog blood pressure decline 40-50%.Radix scutellariae preserved material stomach-filling/kg Stomach-filling, continuous 4 weeks, declines chronic renal hypertension dog blood pressure, heart rate is slack-off by 3 times/day.Baicalin 2 × 10 (- 4) mol/l, In the competitive antagonism adrenaline in guinea pig in vitro aortic article, pulmonary artery item, tracheal strip and atrium dextrum, noradrenaline Contraction caused by element and dopamine, also antagonism isoprel diastole tracheae and increase atrium dextrum spontaneous frequency effect, Prompt baicalin has blocking effect to a, B1, B2 receptor.7, platelet aggregation-against and anticoagulant: baicalein, wogonin, thousand layers It is poly- to can inhibit collagen-induced rat platelet when concentration 1.0mM for paper factor A, skullcapflavone II and Chrysin (chrysin) Collection, Chrysin also have inhibiting effect to the ADP platelet aggregation induced, and baicalein and wogonin are to arachidonic acid-induction Platelet aggregation also has inhibiting effect, baicalein and baicalin to be converted into fibrin to the fibrinogen of thrombin induction Inhibit；Baicalein and baicalin 20,50mg/kg stomach-filling, the rat disseminated intravascular coagulation of induced by endotoxin induction can prevent The reduction of blood platelet and fibrinogen content.8, effect for reducing blood fat: the 10%2ml/ stomach-filling of radix scutellariae infusion, successive administration 7 Week, the rabbit anteserum cholesterol level decline that cholesterol can be made to be fed with.Baicalein, baicalin 100mg/kg stomach-filling, can reduce reality The property tested hyperlipemia rat corn oil-cholesterol-cholic acid is fed with containing for serum free fatty acid, triglycerides and liver triglycerides Amount, skullcapflavone II100mg/kg stomach-filling can reduce the content of serum total cholesterol and liver triglycerides, increase serum high-density The content of lipoproteins-C (HDL-ch), wogonin 100mg/kg stomach-filling, can prevent the deposition of liver triglycerides and increase The content of serum hdl-ch.It is total can to reduce liver to hyperlipemia rat caused by ethyl alcohol for baicalein, baicalin 100mg/kg stomach-filling Cholesterol, free cholesterol and content of triglyceride, wogonin can reduce the level of serum triglyceride, and baicalein can increase Serum hdl-ch content.9, liver protection, cholagogue, anti-oxidant: radix scutellariae methanolic extract 1000mg/kg intraperitoneal injection, to isothiocyanic acid Rat hepatic lesion caused by naphthalene ester (ANIT) has inhibiting effect, can inhibit the increase of serum bilirubin.Radix scutellariae alcohol extract 50, 100mg/kg, baicalin 50,100mg/kg stomach-filling have choleretic effect to rabbit.Wogonin 10 (- 4) -10 (- 6) mol/l is dense In vitro test is spent, has inhibiting effect to rat liver microsomal lipid peroxidation, declines malonaldehyde (MDA) content.Baicalein and Baicalin 2.5 × 10 (- 4) mol/l, 1.0 × 10 (- 4) mol/l, wogonin 2.5 × 10 (- 4) mol/l, skullcapflavone II2.5 × 10 (- 4) mol/l, wogonin -7-O-D glucuronic acid 2.5 × 10 (- 4) mol/l, 1.0 × 10 (- 4) mol/l, There is inhibiting effect to the rat liver homogenate lipid peroxidation of FeC12- vitamin C-ADP mixture induction, keeps the formation of liver MDA aobvious Decline is write, lipid peroxidation caused by mono- ADP of NADPH is also inhibited.10, antitumaous effect: radix scutellariae ether extract is to mouse leukemia L1210 cell has cytotoxicity, effective dose 50 10.4mg/ml, and skullcapflavone II has the half of mouse L1210 cell Effect amount is 1.5 μ g/ml, and baicalin, baicalein and wogonin act on L1210 not significant.11, other effects: baicalein 10, 20mg/kg intravenous injection, has diuresis to anesthetized dog.Radix scutellariae decoction 4g/kg stomach-filling, has rat galactose-cataract anti- Effect is controlled, the formation of cataract can be delayed.Baicalin has an inhibiting effect to Rat Lens aldose reductase, and ID50 is 1.81 × 10(-3)mg/ml.Baicalin 150mg/kg stomach-filling, to blood glucose in diabetic rats level caused by broneomycin without being decreased obviously, But Red Blood Cell Sorbitol Content significantly reduces after treatment, prompts also to play the role of inhibiting aldose enzyme in animal body, it is possible to Prevention and treatment for diabetic complication.Baicalin, baicalein and wogonin 50-125 μ g/ml have Mouse Liver sialidase Inhibiting effect.It is dead that baicalin 100mg/kg, glucuronic acid 43mg/kg subcutaneous injection can fight mouse caused by strychnine It dies, and glucoside member baicalein is invalid, it is believed that the detoxication that the glucuronic acid after baicalin hydrolysis rises.Metabolism of the radix scutellariae to PGs There is wide influence, water extract has inhibiting effect to the biosynthesis of PGs.

In terms of the toxicological action of radix scutellariae, radix scutellariae toxicity is extremely low, and decoction gives rabbit stomach-filling, and alcohol extract intravenous injection is only in Activity weakens, and radix scutellariae preserved material 4g/kg also had no any toxic reaction, and such as infused yellow mustard preserved material 2g/kg vein to dog stomach-filling 8 weeks It penetrates in Healthy Rabbits, first behaves with composure, it is later dead, it is seen that intravenous injection is more much bigger than oral toxicity.Baical Skullcap root P.E muscle and Intravenous injection can make to significantly reduce in the normal rabbits total white blood cells short time.Baicalein, which is injected intraperitoneally, causes the half of mouse Dead amount is 3081mg/kg.Radix scutellariae preserved material 15g/kg/ stomach-filling has vomiting phenomenon to dog, 5g/kg stomach-filling continuous 8 weeks, makes dog loose and watery stool It is soft.

In terms of the chemical component of radix scutellariae, scutellariae,radix contains flavone compound: baicalein (baicalein), neobaicalein (neobaicalein), i.e. skullcapflavone II (skullcapflavoneII), baicalin (baicalin), wogonin (wogonin), wogonoside (wogono-side), oroxylin A (oroxylin, oroxylin A), 7- methoxyl group baicalein (7-methoxbaic alein), skullcapflavone (skullcapflavone) I, dihydro oroxylin A (dihydrooroxylin A), Chrysin (chrysin), 2,5,8- trihydroxy -7- methoxy flavones (2,5,8-trihydroxy-7-methoxy- Flavone), 2,5,8- trihydroxy -6,7- dimethoxy flavone (2,5,8-trihydroxy-6,7- Dimethoxyflavone), 4,5,7- trihydroxy -6- methoxy flavanones (4,5,7-trhydroxy-6- Methoxyflavanone) [5], 2,3,5,6,7- penta hydroxy group flavanones (2,3,5,6,7-pentahydroxyflavanone) Wogonin -5- β-D-Glucose glucoside (wogonin-5- β-D-glucoside), 2- (3- hydroxyl -4- methoxyphenyl)-second Base -1-O- α-L- rhamnopyranosyl (1 → 3)-β-D (4- asafoetide acyl group)-glucose [2- (3-hydroxy-4- Methoxyphenyl) ethyl-1-O- α-L-rhamnosyl (1 → 3)-β-D- (4-feruloyl) glucoside], white poplar Element；- 6-C- α-L-arabinose glucoside (chrysin-6-C- β-D-glucoside-8-C- β-L-arabinoside), Chrysin- 6-C- α-L-arabinose glucoside -8-C- β-D-Glucose glucoside (chrysin-6-C- α-L-arabi-noside-8-C- β-D- Glucoside), (2S) -2,5,6,7- tetrahydroxy flavanones [(2S) -25,6,7-terahydroxyflavanone], 5,7,2, 7- kaempferol (5,7,2,7-tetrahydroxyflavone), 5,8- dihydroxy -6,7- dimethoxy flavones (5,8- Dihydroxy-6,7-drimethoxyflavone), 5,7,4- trihydroxy -8- methoxy flavones (5,7,4-trihydroxy- 8-methoxyflavone), oroxylin A-7-O- glucuronide (oroxylinA-7-O-glucuronide), 5,7, 2- trihydroxy methoxy flavone (5,7,2-trihyroxy-6-methoxyflavone), 5,2- dihydroxy -6,7,8- trimethoxy Base flavones (5,2-dihydroxy-6,7,8-trimethoxyfla-vone), 5- gate of a lane base -7,8- dimethoxy flavone (5- Hydroxy-7,8-dimethoxy-flavone), Norwogonin (norwogonin), dihydro baicalein (dihydro- Baicalin) [11], 5,7,2- trihydroxyflavones (5,7,2-trihydroxyflavone), 5,7,2- trihydroxy -8,6- diformazans Oxygroup flavones (5,7,2-trihydroxy-8,6-dimethoxyflavone), 5,7,2,5- tetrahydroxys -8.6- dimethoxy is yellow Ketone, that is, viscidulin III (5,7,2,5-tetrahydroxy-8,6-dimethoxyflavone, vis-ciduiin III) 5,25- trihydroxy trimethoxy flavones (5,2,5-trihy-droxy-6,7,8-trimethoxyflavone), baicalein -7- O- β-D- glucopyanoside (baicalein-7-O- β-D-glucopyranoside)；5,7,2- trihydroxy -8- methoxyl group is yellow Ketone (5,7,2-trhiyroxy-8-methoxyflavone) i.e. tenaxin II (tenax-in II), 5,2,6- trihydroxy -7, 8- dimethoxy flavone (5,2,6- trihydroxies -6-methoxy-2-phenyl-4H-chromen-4-one (5,7,2-trihydroxy-6-methoxyflavone), 5,7,2,3- kaempferols (5,7,2-trihydroxy-6-methoxyflavone), 5,7,2,3- tetra- gate of a lane base flavones (5,7, 2,3-tetrahydroxyflavone), 3,5,7,2,5,6- pentahydroxyflavones (3,5,7,2,6- Pentahydroxyflavone) i.e. viscidulin I (viscidulin I), (2S) -7,2,6- trihydroxy -5- methoxyl groups are yellow Alkanone [(2S) -7,2,6-trihydroxy-5-methoxyflavanone], 2,6,2,4- tetrahydroxy -6- methoxyl group chalcones (2,6,2,4-tetrahydroxy-6-methoxychalcone) [13], 5,7,2,5- kaempferols (5,7,2,5- Tetrahydroxyflavone), left-handed eriodictyol (eriodictyol), Sculellaria barbata kind plain (rivularin) and Sutellaria viscidula Plain III-2-O- β-D- glucopyanoside (viscidulin III-2-O- β-D-glucoplyranoside) etc..In addition also Containing cupreol (β-sitosterol), campesterol (campesterol) and stigmasterol (stigmasterol) [3].2. Yunnan Scutellariae,radix baicalein containing river rising in Ningxia and flowing into central Shaanxi, baicalein, baicalin, wogonoside, 5,7,2- trihydroxies -6-methoxy-2-phenyl-4H-chromen-4-one, (2S) -5,7,8- Trihydroxy flavanones [(2S) -5,7,8-trihydroxyflavanone]；(2S) -2,5,6,7- tetrahydroxy flavane baicaleins, wood Butterfly element A, scuteamoenoside (scuteamoenoside), (2R, 3R) -2,3,5,7- tetrahydroxy flavanones [(2R, 3R) -2,3, 5,7-tetrahydrox-yflavanone], scuteamoenin (scuteamoenin), (2R, 3R) -3,5,7- trihydroxy flavane Ketone-(2R, 3R) -3,5,7-trihydroxyflavanone], skullcapflavone II, 2,3,5,6,7- pentahydroxyflavones, Chrysin, Cupreol.3. Sutellaria viscidula root contain baicalin, wogonoside, baicalein, wogonin, oroxylin A, neobaicalein, 5, 2- dihydroxy dimethoxy flavone (panicolin) i.e. skullcapflavone I, viscidulin I, II, III.Lijing scutellariae,radix is containing Huang A kind of reed mentioned in ancient books element, wogonin, Chrysin, oroxylin A, tenaxin II glue kilocycle baicalein I.Congener scutellaria rehderiana Diels root contains Rehderianin (rehderianin) I, viscidulin III, baicalin, wogonin, baicalein, oroxylin A, Gansu A kind of reed mentioned in ancient books glucoside is first (ganhuangenin).Scutellaria hypericifolia root contains baicalin, wogonoside, baicalein, viscidulin I, II, oroxylin A and wogonin.

Tabasheer be gramineae plant bambusa texlitis etc. because stinging hole by parasitic India wasp after, and the wound stored up between ring Flow liquid, through cake mass made of dry condensation.Winter harvesting, cuts and takes bamboo stalk, take tabasheer, dry.This product nature output Person is seldom, mostly uses the method for burning bamboo grove greatly, makes bamboo after by sudden-heat, bamboo juice is overflow to be solidified in internode, is then taken and is dried. The ground such as main product Yunnan, Guangdong, Guangxi.With dispelling wind and eliminating dampness, the effect of the warp that relaxes of invigorating blood circulation, cough-relieving.For rheumatic arthralgia, four limbs fiber crops Wood, infantile pertussis, leukorrhea.There is meaning still to control stomach trouble.Tabasheer is sweet in flavor, cold in nature, converge to heart and liver channels.With heat phlegm, The effect of cool heart is calmed the frightened, it is insane for pyreticosis coma, stroke and phlegm confusing heart, the hot frightened epilepsy of children's phlegm, twitch, the morbid night crying of babies, child convulsion, apoplexy Epilepsy, pyreticosis coma.The hot phlegm in Qinghua of tabasheer, the effect of the calming the frightened that clear away heart-fire are similar to bamboo juice and without cold sliding disadvantage.Tabasheer is controlled small The hot infantile convulsion of youngster's phlegm such as embraces imperial ball (key to Therapeutics of Children's Diseases), it is insane to control heap soil or fertilizer over and around the roots of apoplexy phlegm, phlegm heat often with Moschus, arisaema cum bile, cinnabar etc. Epilepsy etc. controls pyreticosis coma and delirium often with the coptis, calamus, Radix Curcumae etc., can be with cow-bezoar, Fructus Forsythiae, Folium Bambosae etc..

In terms of the pharmacological action of tabasheer, to cardiovascular system, Fungus Zhuhuang water decoction extract can make isolated frog heart shrink Power weakens, and heart rate is slack-off.There is direct dilating effect to isolated rabbit ear blood vessel, shows as perfusion flow increase, especially blood vessel is in This effect becomes apparent from when contracture state.Mouse injects Fungus Zhuhuang water decoction extract 3.0g/kg by dorsal sc, to caused by histamine Capillary of skin permeability increase has very significant inhibiting effect.The intravenous 0.5g/kg extract can reduce anesthetized rabbit blood Pressure, mechanism may with influence cardiac output and make arteriolar dilatation, peripheral resistance attenuating is related.Calcium is answered to cardiovascular and blood plasma The influence of time can dramatically and extend blood plasma recalcification time, and in blood coagulation tests, which can extend the clotting time, may be with multiple calcium Time extends related.Analgesic and anti-inflammatory effects, Fungus Zhuhuang water decoction extract 2-3.1g/kg subcutaneous injection, stimulate mouse acetic acid Property pain has preferable analgesic activity.The crystal of extract III (bamboo fungus beetle element) from tabasheer, with 100mg/kg stomach-filling, The significant raising hot plate method in mice threshold of pain of energy, effect is better than Indomethacin (indocin), similar to Sauteralgyl (10mg/kg).Energy The number of writhing response caused by significant reduction acetic acid, also can the swollen degree of vola pedis caused by significant reduction egg white.In addition, fungi bamboo Yellow polysaccharide SB1 and SB2 has certain curative effect through pharmacology preliminary test, to hepatitis.In terms of the clinical application of tabasheer, it is chiefly used in small The hot twitch frightened epilepsy (or even opisthotonos) of youngster, it is clouded in mind, and have the cough thick person of shortness of breath phlegm.With tabasheer heat phlegm, town Static epilepsy, the effect often with reinforcement Qinghua hot phlegm and relieving spasm for resuscitation such as fritillaria, arisaema cum bile, pollen, bombyx batryticatus, uncaria, calamus, and with Demonstrate,prove compatibility antipyretic, side such as Zhu Huangtang.Adult pyreticosis coma, it is also possible to which Zhu's Huang assists heat-clearing.For apoplexy coma due to blocking of the respiratory system (cerebrovascular meaning Outside), performance faints suddenly, is senseless, two fists are held, sound snoring sleep, the not well, phlegm of breathing go out difficulty, with tabasheer slit phlegm, and with Chang Wind side is controlled in Pu, Radix Salviae Miltiorrhizae, Radix Notoginseng etc., side's such as dissolving phlegm.

Mustard seed is dried to heating seed for crucifer mustard.It is cultivation.Main product is in Anhui, Henan, Hebei, mountain The ground such as west, Shandong, Sichuan.Former plant warm and moist weather, it is more drought-enduring, sunlight is liked, is most preferably given birth to fertile wet sand loam It is long, avoid barren, low-lying and ponding environment.It is acrid flavour, warm-natured；It is distributed in lung channel；The function removed obstruction in channels to relieve pain with warm lung slit phlegm benefit gas, dissipating bind Effect.For phlegm asthma cough of trembling with fear, distending pain in the chest and hypochondrium, the stagnant channels and collaterals of phlegm, joint numbness, pain, phlegm-damp oozing in the body, negative subcutaneous ulcer pyogenic infections.Effect warm lung is slitted Phlegm benefit gas, dissipating bind are removed obstruction in channels to relieve pain.Clinic name has (white) mustard seed, fried mustard seed.

In terms of the chemical component of mustard seed, seed containing potassium myronate, myrosase, sinapic acid, sinapine, fat oil, protein, Mucilaginous substance.Gained volatile oil name mustard oil after enzymatic hydrolysis, methyl esters, isopropyl ester, allyl ester, butyl ester, secondary butyl ester containing isothiocyanic acid, Butylene -3- ester, amylene -4- ester, phenyl ester, benzyl ester, phenethyl ester and 3- methyl mercapto propyl ester.Fat oil is the glycerol of a variety of fatty acid Ester, fatty acid are erucic acid, twenty carbene -11- acid, oleic acid, linoleic acid, linolenic acid, palmitinic acid, arachidic acid, stearic acid, behenyl Acid.

In terms of the pharmacological action of mustard seed, stimulation: mustard seed contains potassium myronate, and glucoside itself has no stimulation, but after chance water Effect through myrosase generates volatile oil, and main component is allyl isothiocyanate, there is pungent acid and stimulation.Using In skin, there is warm feeling and be allowed to rubescent, or even causes bubble, warts.It is made into after mustard flour is usually removed fat oil Emplastra sinapis uses, be used as counter-stimulus (dripping of irritant drugs is used in local skin, and effect is not limited only to agents area, and Involve other positions, when generating therapeutic effect, referred to as anti-irritant effect), treatment neuralgia, rheumatalgia, pleurisy and strain Deng.First moistened with the water-wet of tepor using preceding, to reinforce the effect (effect that boiling water then inhibits myrosase) of myrosase.Application time No more than 15-30 minutes, skin sensitivity person can only apply 5-10 minutes.Mustard flour is used as flavoring agent, makes salivary secretion and starch Property increases after enzyme, reduces heart size and heart rate.Gastric mucosa can be stimulated to increase the secretion of gastric juice and pancreatic juice in a small amount, can be delayed sometimes Solve intractable hiccup.It is for oral administration largely to cause vomiting rapidly, it can be used for the treatment of narcotic drugs poisoning.Other effects: cavy Raising can make the effect of thyroid uptake suppressed and SCN- raising in serum with mustard.But SCN- in food is not found What relationship the inhibition of content and intake has.Also there is report that can make its Thyroid Gland Swell to Long term Animal feeding mustard, this can It can be due to having secreted caused by excessive thyrotropic hormone.Rabbit vein injects mustard seed physiological saline leachate, and blood pressure first has gently Degree rise, after then decline, tachypnea.It is for oral administration to make stimulant expectorant, and abdominal pain is treated, it can excessively cause gastrointestinal tract inflammation.

The present invention is by simple Chinese prescription, or even passes through simple application method, it has been unexpectedly discovered that institute It obtains Chinese medicine composition and excellent physiologic effect is presented, can be particularly used for treatment cough with lung heat, phlegm is contained, wheezed, phlegm is yellow, tongue Red yellow tongue fur is used for clearing lung, eliminating phlegm, relieving cough & asthma；More particularly into can be used for pediatric patient or child patient cough with lung heat, Phlegm is contained, is wheezed, phlegm is yellow, red tongue with yellowish fur, is used for clearing lung, eliminating phlegm, relieving cough & asthma.

Specific embodiment

The present invention can be further described by the following examples, however, the scope of the present invention and unlimited In following embodiments.One of skill in the art, can be with it is understood that under the premise of without departing substantially from the spirit and scope of the present invention Various change and modification are carried out to the present invention.The present invention carries out the material and test method arrived used in test general And/or specific description.Although to realize the present invention many materials and operating method used in purpose be it is known in the art that But the present invention is still described in this detail as much as possible.In the present invention, as not specified, medicinal material used is medicinal material Dry product, and meet States Pharmacopoeia specifications.In the example below the present invention, the inventory of various materials is in parts by weight, and When actually feeding intake, whole material total weights of every batch of are no less than 5 kilograms.

Embodiment 1: Chinese medicine composition is prepared

Formula: 1 parts by weight of radix scutellariae, 1 parts by weight of tabasheer, 0.15 parts by weight of mustard seed.

Preparation method: each medicinal material is respectively crushed into the fine powder that can pass through 120 meshes, is uniformly mixed by formula rate；

Half mixed powder is taken, the honey of 1/2 amount is added, is made for oral honeyed bolus, sealing, every based on radix scutellariae 0.2g；

The other half mixed powder is taken, the vinegar of 1/2 amount is added, is sufficiently mixed, is modulated into paste, paste is placed in drug paste, is made At the patch that can be for external application, sealing, every patch 0.5g based on radix scutellariae.

Embodiment 2: Chinese medicine composition is prepared

Formula: 1 parts by weight of radix scutellariae, 5 parts by weight of tabasheer, 0.05 parts by weight of mustard seed.

Preparation method: each medicinal material, which is respectively crushed into, can lead to the fine powder sieved with 100 mesh sieve, be uniformly mixed by formula rate；

Half mixed powder is taken, the honey of 1/5 amount is added, is made for oral honeyed bolus, sealing, every based on radix scutellariae 0.2g；

The other half mixed powder is taken, the vinegar of 1/2 amount is added, is sufficiently mixed, is modulated into paste, paste is placed in drug paste, is made At the patch that can be for external application, sealing, every patch 0.25g based on radix scutellariae.

Embodiment 3: Chinese medicine composition is prepared

Formula: 1 parts by weight of radix scutellariae, 0.2 parts by weight of tabasheer, 2 parts by weight of mustard seed.

Preparation method: each medicinal material is respectively crushed into the fine powder that can pass through 80 meshes, is uniformly mixed by formula rate；

Half mixed powder is taken, the honey of 1/3 amount is added, is made for oral honeyed bolus, sealing, every based on radix scutellariae 0.2g；

The other half mixed powder is taken, the water of 1/1.5 amount is added, is sufficiently mixed, is modulated into paste, paste is placed in drug paste, is made At the patch that can be for external application, sealing, every patch 0.25g based on radix scutellariae.

Embodiment 4: Chinese medicine composition is prepared

Formula: 1 parts by weight of radix scutellariae, 2.5 parts by weight of tabasheer, 0.1 parts by weight of mustard seed.

Half mixed powder is taken, is directly loadable into hard capsule, is made for oral capsule, sealing, every based on radix scutellariae 0.2g；

The other half mixed powder is taken, the yellow rice wine of 1/1.5 amount is added, is sufficiently mixed, is modulated into paste, paste is placed in drug paste, The patch that can be for external application is made, seals, every patch 0.25g based on radix scutellariae.

Embodiment 5: Chinese medicine composition is prepared

Formula: 1 parts by weight of radix scutellariae, 0.25 parts by weight of tabasheer, 1 parts by weight of mustard seed.

Half mixed powder is taken, the honey of 1/1.2 amount is added, is made for oral honeyed bolus, sealing, every based on radix scutellariae 0.2g；

Embodiment 6: Chinese medicine composition is prepared

Preparation method: taking the medicinal material of recipe quantity, adds water boiling and extraction 2 hours of 6 times of amounts, takes decocting liquid, and the dregs of a decoction add 5 times of amount water to decoct 1.5 hours, decocting liquid is taken, collecting decoction filters, and is concentrated under reduced pressure, and spray drying obtains extract, sealed package, every bag is equivalent to Radix scutellariae 1g, as electuary (granule), for oral.

Embodiment 7: Chinese medicine composition is prepared

Formula: 1 parts by weight of radix scutellariae (wine), 2.5 parts by weight of tabasheer, mustard seed (yellow, to fry) 0.1 parts by weight.

Preparation method: taking the medicinal material of recipe quantity, adds 65% alcohol reflux of 6 times of amounts to extract 2 hours, takes extracting solution, the dregs of a decoction add 5 times It measures 65% alcohol reflux to extract 1.5 hours, takes extracting solution, combined extract, filtering is concentrated under reduced pressure and removes ethyl alcohol, spray drying, Weight sucrose is doubled, softwood processed is pelletized, and dry, sealed package, every bag is equivalent to radix scutellariae 1g, can as electuary (granule) For oral.

Embodiment 8: Chinese medicine composition is prepared

Formula: 1 parts by weight of radix scutellariae (stir-fry), 0.25 parts by weight of tabasheer (schizostachyum chinense Rendle), 1 parts by weight of mustard seed (Huang).

Preparation method: taking the medicinal material of recipe quantity, adds 75% alcohol reflux of 6 times of amounts to extract 2 hours, takes extracting solution liquid, the dregs of a decoction add 5 75% alcohol reflux of amount extracts 2 hours again, takes extracting solution, combined extract, and filtering is concentrated under reduced pressure removing ethyl alcohol, obtains clear cream, often 100 grams are equivalent to 10 grams of radix scutellariae, add clear cream amount 25%PEG400 and clear cream amount 20%PEG4000, are uniformly mixed, ointment is made It is for external application.

Embodiment 9: Chinese medicine composition is prepared

Formula: 1 parts by weight of radix scutellariae (charcoal), 2 parts by weight of tabasheer, 0.1 parts by weight of mustard seed.

Preparation method: using the water of 6 times of amounts to extract 2 times each medicinal material respectively, combined extract, and filtrate is concentrated in filtering, spraying dry It is dry, 4 kinds of extract powders are obtained, then add water that oral solution is made extract, every 5ml is equivalent to 0.25 gram of radix scutellariae.

Embodiment 10: Chinese medicine composition is prepared

Formula: 1 parts by weight of radix scutellariae, 0.5 parts by weight of tabasheer, 0.5 parts by weight of mustard seed.

Preparation method: the water that radix scutellariae is measured with 6 times extracts 2 times, combined extract；The water for separately measuring tabasheer and mustard seed with 6 times It extracts 2 times, combined extract；Merge two kinds of extracting solutions, filter, filtrate is concentrated, spray drying obtains extract powder, then Oral solution is made in extract plus water, every 5ml is equivalent to 0.25 gram of radix scutellariae.

Embodiment 11: Chinese medicine composition is prepared: referring to the formula and preparation method of embodiment 6, the difference is that in the composition not Radix scutellariae, tabasheer or mustard seed are added, obtains three kinds of compositions, they lack a kind of Chinese medicine respectively.

Embodiment 12: the Sinapine Thiocyanate in HPLC method measurement composition is used

A, measuring method:

The preparation of test solution: taking the test sample for being equivalent to mustard seed medicinal material 1g, accurately weighed, sets in stuffed conical flask, Methanol 50ml, calcium chloride 100mg, 20 μ l of glacial acetic acid is added, with power 250W, frequency 20kHz ultrasonic treatment 20 minutes, filters, Filter residue again with methanol is extracted three times with method, and filtrate merges；Solvent is recovered under reduced pressure to doing, residue adds flowing phased soln, is transferred to In 50ml measuring bottle, be diluted to scale with mobile phase, shake up, filter, take subsequent filtrate to get；

B: measurement result

Using the above HPLC method, mustard seed medicinal material and various compositions prepared by the present invention are measured, as the result is shown:

(1) in variety classes/batch mustard seed medicinal material the content of Sinapine Thiocyanate in 0.84~1.13% range It is interior, it is all satisfied pharmacopeic limits requirement；

(2) 1~embodiment of embodiment, 10 resulting composition is measured respectively, is calculated wherein Sinapine Thiocyanate actual measurement and is contained Amount；Determination of Sinapine Thiocyanate in mustard seed medicinal material inventory and mustard seed medicinal material is discussed further accordance with each combination physics, calculates the group Close Sinapine Thiocyanate theoretical content in object；To survey content divided by theoretical content multiplied by 100% gained percentage, as The rate of recovery of Sinapine Thiocyanate；1~embodiment of embodiment, 10 resulting composition all compositions Sinapine Thiocyanate For the rate of recovery in 96~101% ranges, display methods has excellent results；

(3) 11 gained radix scutellariae of embodiment+two kinds of compositions of mustard seed and tabasheer+mustard seed are measured respectively according to above (2) method, For the rate of recovery of two kinds of composition Sinapine Thiocyanates in 97~99% ranges, display methods has excellent results；

In the other complementary testing of the present invention, referring to the HPLC method of above-described embodiment 12, different is only test sample (a) does not add calcium chloride and glacial acetic acid, (b) do not add calcium chloride, (c) does not add glacial acetic acid when the preparation of solution, measures mustard seed Medicinal material and various compositions, as the result is shown:

(1) (a), (b) and (c) three kinds of conditions are used, Sinapine Thiocyanate in variety classes/batch mustard seed medicinal material Content is all satisfied pharmacopeic limits requirement, and data are substantially the same manner as Example 12 in 0.81~1.11% range；

(2) (a), (b) and (c) three kinds of conditions are used, measure 1~embodiment of embodiment, 10 resulting composition respectively, are implemented The rate of recovery of 1~embodiment of example, 10 all compositions Sinapine Thiocyanate is shown a large amount of in 43~58% ranges Target substance undetermined arrives；

(3) (a), (b) and (c) three kinds of conditions are used, measure 11 gained radix scutellariae of embodiment+mustard seed and tabasheer+mustard respectively Sub two kinds of compositions, the rate of recovery comprising radix scutellariae+mustard seed composition Sinapine Thiocyanate is in 48~53% ranges, packet In 98~102% ranges, display methods may be the rate of recovery of the composition Sinapine Thiocyanate containing tabasheer+mustard seed The presence of radix scutellariae and to purpose object measurement have an impact.

(4) referring to example 1 above~5 and the formula and preparation method of embodiment 7~9, different is only not add radix scutellariae, Obtain the composition for being not added with radix scutellariae；(a), (b) and (c) three kinds of conditions are used, measure sinapine sulphur in resulting composition respectively The rate of recovery of cyanate, as a result in 97~102% ranges, it may be the presence of radix scutellariae and to purpose that this, which has further been confirmed, Object measurement has an impact, however this influence can be by adding calcium chloride and glacial acetic acid during preparing test solution Method overcomes.

Experimental example 1: curative effect of the present composition to infantile cough's asthma

1, case selection: doctor trained in Western medicine diagnosis meets the diagnostic criteria of 2002 " practical paidonosology " cough, asthmas in the 7th edition, in Doctor's diagnosis meets examining for the Standards for Diagnosis and Curative Effect of Chinese Medical Symptom syncope due to pathogenic cold drink volt lung card of State Administration of Traditional Chinese Medicine's publication in 1994 Disconnected standard.Clinical manifestation is cough and asthma, wheezing sound in the throat, and phlegm is white and glutinous or thin more foams, to the cold what for, pale white complexion, tongue nature Light red white,slippery and moist fur, floating-rolling pulse.Patient 180 of the age 1.5~8 years old are chosen, are randomly divided into 2 treatment groups, every group 90, sample Without significant difference.

2, treatment method: two groups of therapeutic component, first group of 1 posts agent of application embodiment is applied to Tiantu cave, and one time a day 1 Patch, 7 are 1 course for the treatment of；Second group of 6 preparation of application embodiment is twice daily equivalent to 0.25 gram of radix scutellariae every time, and 7 are 1 course for the treatment of.

3, efficacy determination: referring to " tcm internal medicine common disease practice guidelines Chinese medical disease part ".It cures: occasionally having and tussicula, But being not required to administration can be relieved, or occasionally has and tussicula, and clinical symptoms disappear；Effective: clinical symptoms are substantially reduced, and cough still has timing to send out Make, but number substantially reduces；Effective: cough timing breaks out, but the opposite reduction of number；It is invalid: coughing fit number or symptom without Improve and even aggravates.

4, result: treatment one course for the treatment of after, according to above-mentioned curative effect determinate standard count each group therapeutic effect, statistical result and Total effective rate is as follows:

Embodiment 1: 56,22 effective, 9 effective, 3 invalid, total effective rate 96.7% are cured；Embodiment 6: it cures 53,27 effective, 8 effective, 2 invalid, total effective rate 97.8%.

As can be seen from the above results, embodiment 1,6 two kind of composition are administered through different approaches to infantile cough's asthma Significant effect, effective percentage reach 96.0% or more.

Experimental example 2: the pharmacodynamic study of present composition clearing lung, eliminating phlegm, relieving cough & asthma

This test mainly to the present composition carried out cough-relieving, resolving sputum and relieving asthma aspect pharmacodynamic study, to for Clinical application present composition clearing lung, eliminating phlegm, relieving cough & asthma provides tentative theory foundation.

1, experimental material

(1) drug and reagent: 6,9,10 composition of the embodiment of the present invention, GUILONG KECHUANNING JIAONANG (Anhui osmanthus dragon medicine company)； Dextromethorphan hydrobromide capsule, Carbocisteine Tablets, aminophylline tablets are commercial preparation.Ammonium hydroxide, phenol red, sodium bicarbonate are analysis Pure reagent.

(2) animal: ICR mouse, cavy are provided by Jilin University's preclinical medicine institute Experimental Animal Center, animal licensing Number: SCXK (Ji) 2007-0003.

(3) instrument and equipment: the multi-functional instrument to induce to cough and asthma of YLS-8A, Shandong Academy of Medical Sciences's equipment station；DT-100 points Analyse balance, Beijing Optical Instrument Factory；721 spectrophotometers, Shandong Gaomi Caihong Analytical Instrument Co., Ltd.；TD5A-WS low speed Desk centrifuge, Changsha Xiang Yi centrifuge Instrument Ltd.；Binocular biological microscope, Japanese OLYMPUS.

(4) statistical method: each group result indicates that data are with the analysis of DAS2.1.1 software statistics, comparison among groups with (x ± s) It is examined with t.

2, method and result

(1) cause the influence of mouse cough to concentrated ammonia liquor spray-on process:

Taking 18~22g ICR mouse 60, half male and half female is randomly divided into 6 groups by weight, and every group 10, i.e. blank control Group, 6 composition of embodiment (being equivalent to 0.5g radix scutellariae/kg the weight of animals), 9 composition of embodiment (it is dynamic to be equivalent to 0.5g radix scutellariae/kg Object weight), 10 composition of embodiment (being equivalent to 0.5g radix scutellariae/kg the weight of animals), GUILONG KECHUANNING JIAONANG (0.6g/kg) group and Dextromethorphan hydrobromide (0.015g/kg) group.Daily stomach-filling (ig) is administered once, and continuous 5 days, blank control group gave isometric(al) Distilled water.Each group mouse fasting 16h before testing, 30min, mouse is put into and lures cough to draw asthma added with concentrated ammonia liquor after the last administration In instrument, it is atomized 15s, is observed and recorded in the cough latent period (time coughed occur to animal since spraying) and 2min of mouse It coughs number [.2 editions Shanghai Scientific & Technical Publishers of Li Yi Kui herbal pharmacology experimental methodology [M], 2006:428,771,734].Group Close object to concentrated ammonia liquor spray-on process cause mouse cough influence (x ± s) as a result, each group incubation period (s) respectively it is as follows: blank Control group 22.2 ± 5.3,6 36.4 ± 11.2* of composition of embodiment, 9 34.2 ± 9.1* of composition of embodiment, embodiment 10 combine 35.7 ± 14.1* of object, GUILONG KECHUANNING JIAONANG 33.4 ± 7.3* of group, 36.3 ± 8.6* of dextromethorphan hydrobromide group；The cough of each group Number of coughing is (secondary) as follows respectively: blank control group 52.3 ± 11.3,6 32.3 ± 10.6** of composition of embodiment, embodiment 9 combine 31.1 ± 9.7** of object, embodiment 10 33.6 ± 8.4** of composition, GUILONG KECHUANNING JIAONANG 41.7 ± 12.3* of group, hydrobromic acid are right 32.4 ± 11.7** of dextromethorphan group；Wherein, compared with blank control group, P < 0.01 * P < 0.05, * *.

The result shows that present composition group, GUILONG KECHUANNING JIAONANG group and dextromethorphan hydrobromide group can extend it is small Mouse cough latent period, reducing cough number has significant difference (P < 0.05 or P < 0.01) compared with blank control group.In addition The antitussive action of 11 three kinds of compositions of gained of embodiment is investigated referring to this test method, as the result is shown the incubation period of three kinds of compositions Mean value is within the scope of 19~23 seconds, and number mean value of coughing is in 50~53 underranges, without statistics compared with blank control group Difference shows that four kinds of medicinal material combinations lack any one and cannot obtain ideal cough suppressing effect.

(2) to the influence of Respiratory Tract of Mice phenols contents

Taking ICR mouse 60 of 18~22g, half male and half female is randomly divided into 6 groups by weight, and every group 10, i.e. blank control Group, 6 composition of embodiment (being equivalent to 0.5g radix scutellariae/kg the weight of animals), 9 composition of embodiment (it is dynamic to be equivalent to 0.5g radix scutellariae/kg Object weight), 10 composition of embodiment (being equivalent to 0.5g radix scutellariae/kg the weight of animals), GUILONG KECHUANNING JIAONANG (0.6g/kg) group and Carbocisteine (0.2g/kg) group, daily ig are administered once, and continuous 7 days, blank control group gave the distilled water of isometric(al).Before test Each group mouse fasting 16h, 30min, is injected intraperitoneally 5% phenol red solution 0.2mL/10g weight, after injection is phenol red after the last administration 30min, air embolism put to death animal, remove tracheae surrounding tissue, cut one section of gas from thyroid cartilage down toward tracheae bifurcation Pipe, is put into the test tube for filling 3mL physiological saline, 5% sodium bicarbonate 0.1mL tune pH value is added, with 721 type spectrophotometrics Meter, wavelength 546nm survey OD value, do a standard curve with phenol red, and calculating phenol red content according to curve, [Qi Chen's herbal pharmacology is studied Beijing methodology [M]: People's Health Publisher, 1993:642].Phenol red standard curve: Y=0.0595X+0.0053, wherein R =0.9999, Y are OD value, and X is phenol red content (μ g/mL).Influence (x of the various compositions to Respiratory Tract of Mice phenols contents ± s) it is indicated with phenol red concentration (μ g/mL), as a result distinguish as follows: blank control group 2.08 ± 0.74,6 composition 3.87 of embodiment ± 1.51*, 9 3.98 ± 1.24* of composition of embodiment, 10 3.81 ± 1.66* of composition of embodiment, GUILONG KECHUANNING JIAONANG group 3.34 ± 0.86*, 3.27 ± 1.17* of carbocisteine group.Wherein, compared with blank control group, P < 0.05 *.The result shows that this hair Bright composition group, high dose group, GUILONG KECHUANNING JIAONANG group and carbocisteine group can increase Respiratory Tract of Mice phenols contents, Compared with blank control group, there is significant difference (P < 0.05).11 three kinds of gained of embodiment is investigated referring additionally to this test method The resolve phlegm effect of composition, phenol red concentration (μ g/mL) mean value of three kinds of compositions is in 2.1~2.2 ranges as the result is shown, with sky The white more equal no difference of science of statistics of control group shows that four kinds of medicinal material combinations lack any one and cannot obtain ideal resolving sputum effect Fruit.

(3) influence of cavy allergic asthma is induced egg protein

60 cavys are taken, 200~350g of weight is randomly divided into 6 groups by weight, and every group 10, i.e. model control group implement 6 composition of example (being equivalent to 0.3g radix scutellariae/kg the weight of animals), 9 composition of embodiment (are equivalent to 0.3g radix scutellariae/kg animal body Again), 10 composition of embodiment (being equivalent to 0.3g radix scutellariae/kg the weight of animals), GUILONG KECHUANNING JIAONANG (0.35g/kg) group and ammonia Theophylline piece (0.045g/kg) group.Reference literature [it is healthy and strong, it waits to disappear and breathes heavily the deposited mountain influence [J] to Airway in Asthmatic Guinea Pigs inflammation of patch Eastern university of TCM's journal, 2003,27 (1): 66-67] modeling method and slightly improve: 10% ovum is injected intraperitoneally in groups of animals Albumen normal saline solution 1mL/ is only.Start ig administration, 1 time a day, continuous 7 days after 2 weeks.Since the 17th day with draw asthma instrument spray 1% egg protein normal saline solution of mist makes animal sucking 15min excitation, draws asthma 1 time, totally 3 times every other day.It is excited in last time Its asthmatic latent period is recorded, i.e., (shows as cough to asthma attack since spraying, expiratory dyspnea, irritated, twitch, fall very To death) time, do not occur calculating with 6min for asthma attack more than 6min.

After last excitation for 24 hours, with yellow Jackets anaesthetized guinea pig, pars cervicalis tracheae is isolated, gastric perfusion needle is inserted into tracheae, into Row bronchoalveolar lavage extracts 6ml physiological saline, is slowly injected into every time, retains pumpback after 20s, is repeated 3 times, takes a small amount of filling Washing lotion (BALF) is added 1% hydrochloric acid of equivalent and makees white blood cell count(WBC).Composition to cavy egg protein asthmatic latent period influence (x ± S) as a result, the incubation period (s) of each group it is as follows respectively: model control group 32.5 ± 14.4,6 composition 59.3 of embodiment ± 18.1*, 9 63.1 ± 10.4* of composition of embodiment, 10 66.2 ± 14.7* of composition of embodiment, GUILONG KECHUANNING JIAONANG group 57.5 ± 16.4*, 63.4 ± 21.3* of aminophylline group；The white blood cell count(WBC) (× 10 of each group⁹/ L) as follows respectively: model control group 11.33 ± 4.21,6 2.02 ± 0.74** of composition of embodiment, 9 2.21 ± 0.67** of composition of embodiment, 10 composition 1.93 of embodiment ± 0.84**, GUILONG KECHUANNING JIAONANG 2.96 ± 0.73** of group, 3.83 ± 1.18** of aminophylline group；Wherein, with model control group Compare, P < 0.01 * P < 0.05, * *.The result shows that present composition group, GUILONG KECHUANNING JIAONANG group and the equal energy of aminophylline group Prolonged guinea pig asthmatic latent period, reduce cavy BALF in quantity of leucocyte, compared with model control group, have significant difference (P < 0.05 or P < 0.01).The antiasthmatic effect of 11 three kinds of compositions of gained of embodiment is investigated referring additionally to this test method, as the result is shown The incubation period mean value of three kinds of compositions is within the scope of 33~38 seconds, white blood cell count(WBC) (× 10⁹/ L) mean value is in 9.2~9.7 ranges Interior, the equal no difference of science of statistics compared with blank control group shows that four kinds of medicinal material combinations lack any one and cannot obtain ideal Antiasthmatic effect.

It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie In the case where without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power Benefit requires rather than above description limits, it is intended that all by what is fallen within the meaning and scope of the equivalent elements of the claims Variation is included within the present invention.

Claims

1. the method that pair composition carries out quality testing comprising using high performance liquid chromatography to the sinapine sulphur in composition Cyanate carries out assay, including operates as follows:

It is surveyed according to the specification of Section of 0512 contained high performance liquid chromatography of four general rules of the version Pharmacopoeia of the People's Republic of China in 2015 It is fixed；

Chromatographic condition and system suitability: using octadecylsilane chemically bonded silica as filler；With acetonitrile -0.08mol/L Potassium dihydrogen phosphate is using the mixed liquor of volume ratio 10:90 as mobile phase；Detection wavelength is 326mn；Number of theoretical plate presses sinapine Peak, which calculates, is not less than 3000；

The preparation of reference substance solution: taking Sinapine Thiocyanate reference substance appropriate, accurately weighed, adds mobile phase that every 1ml is made and contains The solution of 0.2mg to get；

The preparation of test solution: taking the test sample for being equivalent to mustard seed medicinal material 1g, accurately weighed, sets in stuffed conical flask, is added Methanol 50ml, with power 250W, frequency 20kHz ultrasonic treatment 20 minutes, filtration, filter residue again with methanol extracted filter three times with method Liquid merges；Solvent is recovered under reduced pressure to doing, residue adds flowing phased soln, is transferred in 50ml measuring bottle, it is diluted to scale with mobile phase, Shake up, filter, take subsequent filtrate to get；Alternatively, for example, the preparation of test solution is carried out according to such as under type: taking and be equivalent to The test sample of mustard seed medicinal material 1g, it is accurately weighed, it sets in stuffed conical flask, methanol 50ml, calcium chloride 100mg, 20 μ of glacial acetic acid is added L, with power 250W, frequency 20kHz ultrasonic treatment 20 minutes, filtration, filter residue again with methanol was extracted three times with method, and filtrate merges； Solvent is recovered under reduced pressure to doing, residue adds flowing phased soln, is transferred in 50ml measuring bottle, is diluted to scale with mobile phase, shakes up, and filters Cross, take subsequent filtrate to get；

Measuring method: it is accurate respectively to draw reference substance solution and each 10 μ l of test solution, inject liquid chromatograph, measurement, by outer Mark method is with Sinapine Thiocyanate, that is, C16H24NO5SCN content in calculated by peak area test sample.

2. a kind of Chinese medicine composition, by including that following Chinese medicine and optional pharmaceutic adjuvant are prepared: radix scutellariae, India Yellow, mustard seed.

3. Chinese medicine composition according to claim 2, it is characterised in that:

It is by including that the Chinese medicine of following ratio is prepared: 1 parts by weight of radix scutellariae, 0.2~5 parts by weight of tabasheer, mustard seed 0.05 ~2 parts by weight；

It is by including that the Chinese medicine of following ratio is prepared: 1 parts by weight of radix scutellariae, 0.25~2.5 parts by weight of tabasheer, mustard seed 0.1~1 parts by weight；

It is by including that the Chinese medicine of following ratio is prepared: 1 parts by weight of radix scutellariae, 0.5~2 parts by weight of tabasheer, mustard seed 0.1~ 0.5 parts by weight；And/or

It is by including that the Chinese medicine of following ratio is prepared: 1 parts by weight of radix scutellariae, 1 parts by weight of tabasheer, 1 parts by weight of mustard seed.

4. Chinese medicine composition according to claim 2, it is characterised in that:

The radix scutellariae be in the form of medicinal powder or be in the form of its extract or be with its with it is other a kind of or A variety of medicinal materials extract extract obtained form together and are added in the composition；

The tabasheer is in the form of medicinal powder or to be in the form of its extract or be with itself and other one kind Or a variety of medicinal materials extract extract obtained form together and are added in the composition；And/or

The mustard seed be in the form of medicinal powder or be in the form of its extract or be with its with it is other a kind of or A variety of medicinal materials extract extract obtained form together and are added in the composition.

5. Chinese medicine composition according to claim 2, it is characterised in that:

The medicinal powder is at least can be by the powder of 80 meshes, or can at least lead to the powder sieved with 100 mesh sieve, or Person is at least can be by the powder of 120 meshes；

The extract, whether the extract of single medicinal material or a variety of medicinal materials extract extract obtained together, extraction it is molten Agent can be selected from: the ethyl alcohol that water, ethyl alcohol, ethanol water such as concentration are 10~90%；

The extraction can be any extracting mode or combinations thereof well known to pharmaceutical field, such as, but not limited to: impregnate, decoct, Diacolation etc., preferably removes solvent after extraction；

The powder such as can for example be eaten with water, ethyl alcohol such as yellow rice wine, acetic acid optionally with liquid or semisolid auxiliary material combination Vinegar and its aqueous solution etc. combination form paste and are for external application；

It is external preparation；For example, the external preparation is the forms such as patch, ointment, cream；For example, the external preparation is Make the powder of the medicinal material and liquid or semisolid auxiliary material be tuned into paste (such as the weight ratio 1~5 of medicinal powder and Auxiliary Liquid Material: 1)；And/or

It is oral preparation；For example, the oral preparation is in granule, tablet, capsule, oral solution, electuary, pill, is sprayed The forms such as agent.

6. Chinese medicine composition according to claim 2 is external preparation or oral preparation, is to shine to include the following steps, namely It is prepared: making each pulverizing medicinal materials at fine powder, or make each medicinal material individually or any combination extracts to obtain extract, then by institute It obtains fine powder or extract and external preparation or oral preparation is prepared with preparation process, optionally added in preparation medicinal auxiliary Material.

It is external preparation or oral preparation 7. the method for preparing any one of claim 2~6 Chinese medicine composition, It shines and includes the following steps, namely to be prepared: making each pulverizing medicinal materials of radix scutellariae, tabasheer, mustard seed at fine powder, or make each medicinal material Individually or any combination extracts to obtain extract, then by gained fine powder or extract with preparation process be prepared external preparation or Person's oral preparation optionally adds pharmaceutic adjuvant in preparation.

8. method according to claim 7, it is characterised in that:

The Chinese medicine composition is external preparation；For example, the external preparation is the forms such as patch, ointment, cream；For example, The external preparation is that the powder of the medicinal material and liquid or semisolid auxiliary material is made to be tuned into paste (such as the weight of medicinal powder and Auxiliary Liquid Material Amount is than being 1~5:1)；

The Chinese medicine composition is oral preparation；For example, the oral preparation is in granule, tablet, capsule, oral solution, punching The forms such as agent, pill, spray；And/or

The Chinese medicine composition is external preparation or oral preparation, shines and includes the following steps, namely to be prepared: being made each Pulverizing medicinal materials are at fine powder, or make each medicinal material individually or any combination extracts to obtain extract, then by gained fine powder or extract External preparation or oral preparation is prepared with preparation process, optionally adds pharmaceutic adjuvant in preparation.

9. including purposes of the combination of following Chinese medicine in the drug that preparation is used for clearing lung, eliminating phlegm, relieving cough & asthma, or preparing For treating cough with lung heat, phlegm is contained, wheeze, phlegm is yellow, the purposes in red tongue with yellowish fur drug: radix scutellariae, tabasheer, mustard seed.

10. purposes according to claim 9, the drug is suffered from for pediatric patient clearing lung, eliminating phlegm, relieving cough & asthma or for children Person cough with lung heat, phlegm contain, wheeze, phlegm is yellow, red tongue with yellowish fur；Alternatively, the drug is any one of claim 1~6 Chinese medicine Composition；Alternatively, the drug is the Chinese medicine composition that claim 7~8 the method is prepared.