CN109234195A - A kind of rice field complex microbial inoculum - Google Patents
A kind of rice field complex microbial inoculum Download PDFInfo
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- CN109234195A CN109234195A CN201811141484.6A CN201811141484A CN109234195A CN 109234195 A CN109234195 A CN 109234195A CN 201811141484 A CN201811141484 A CN 201811141484A CN 109234195 A CN109234195 A CN 109234195A
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- bacillus
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
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Abstract
A kind of rice field complex microbial inoculum includes three kinds of photosynthetic bacteria, bacillus subtilis, three kinds of strains of bacillus licheniformis and amino acid, zinc sulfate, calcium chloride compounds；Above-mentioned strain, compound are added dilution pure water, and composite bacteria agent are made according to following mass ratio: photosynthetic bacteria 10 ~ 20%, bacillus subtilis 10 ~ 15%, bacillus licheniformis 5 ~ 8%, amino acid 6 ~ 8%, zinc sulfate 0.5 ~ 1%, calcium chloride 1 ~ 3%, pure water 50 ~ 55%；Three kinds of photosynthetic bacteria, bacillus subtilis, bacillus licheniformis strain living bacteria counts contained in composite bacteria agent are greater than 200,000,000/liter；The best proportion of strain, compound and dilution pure water used in composite bacteria agent are as follows: photosynthetic bacteria 15%, bacillus subtilis 15%, bacillus licheniformis 7%, amino acid 6%, zinc sulfate 1%, calcium chloride 1%, pure water 55%；Photosynthetic bacteria used can be capsula Rhodopseudomonas, Rhodopseudomonas palustris.
Description
Technical field
The invention belongs to soil improving environment fields, and in particular to a kind of rice field complex microbial inoculum.
Background technique
As large agricultural country, the crop products of production also carry out a large amount of export sales in addition to self-sustaining in China, so agriculture
Crop product occupies very important status in China economic field；As the demand of crop products in recent years increases, state
Family advocates agricultural production energetically, causes people in order to improve crop yield, largely achievees the purpose that volume increase using chemical fertilizer；Together
When, the unreasonable use of chemical fertilizer in rural activity, the unreasonable exploitation of agricultural resource are also resulted in, the structure for causing soil is broken
Bad, Trace Element Imbalance, fertility decline, soil texture decline；
Currently, the soil remediation method that microorganism formulation has been used as one kind novel is gradually instead of chemical elements such as chemical fertilizer to soil
The improvement of earth；Microorganism formulation ameliorative way is promoted and enhancement microbiological using reacting to each other between microorganism fungus kind
Metabolic function generates a large amount of beneficial bacterium, and the activity of the toxin in soil, harmful bacteria is made to reduce or be degraded to harmless components；It is micro-
Bacteria agent can secret out of the organic principles such as the degrading enzyme for decomposition and inversion harmful substance, accelerate difficulty or ease degradation in soil
Fertilizer residue promotes oxygen cycle by the ecological environment of balanced soil, improves production estimation environment, integrates to soil
It administers, improves crop, yield；Improve soil texture, synergy volume increase with microorganism formulation, it has also become modern agriculture hair
The inexorable trend of exhibition；
Existing common microorganism inoculant on the market is mostly that strain is single, though implementable soil in a wide variety of different scenarios
In, also have the effect of certain improvement soil, but effect is difficult to reach expected, can only be handled for single problem；
Second is that the survival period in soil is shorter, effect is obvious in a short time, improvement soil environment that can not be permanently effective, needs to carry out anti-
Multiple use come maintain reinforce administer effect.
Summary of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of rice field complex microbial inoculum, Neng Gouchang
Phase effectively improves soil texture, solves soil issues from root.
The technical scheme is that a kind of rice field complex microbial inoculum, includes photosynthetic bacteria, bacillus subtilis
Three kinds of bacterium, three kinds of strains of bacillus licheniformis and amino acid, zinc sulfate, calcium chloride compounds；Above-mentioned strain, compound are added
Enter dilution pure water, and composite bacteria agent be made according to following mass ratio:
Photosynthetic bacteria 10 ~ 20%,
Bacillus subtilis 10 ~ 15%,
Bacillus licheniformis 5 ~ 8%,
Amino acid 6 ~ 8%,
Zinc sulfate 0.5 ~ 1%,
Calcium chloride 1 ~ 3%,
Pure water 50 ~ 55%；
Wherein, three kinds of photosynthetic bacteria, bacillus subtilis, bacillus licheniformis strains have contained in the composite bacteria agent
Imitate viable count be greater than 200,000,000/liter.
The best proportion of strain, compound and dilution pure water used in composite bacteria agent are as follows: photosynthetic bacteria 15%,
Bacillus subtilis 15%, bacillus licheniformis 7%, amino acid 6%, zinc sulfate 1%, calcium chloride 1%, pure water 55%.
Photosynthetic bacteria used can be capsula Rhodopseudomonas, Rhodopseudomonas palustris.
Bacillus licheniformis used can be bacillus megaterium, side bacillus.
Amino acid used can be glutamic acid, glycine, threonine, phenylalanine.
Beneficial effect caused by this programme is:
Complex microbial inoculum environmental protection provided by the present invention for rice field is easy to get, and permanently effective can improve to soil,
And significant effect；Include photosynthetic bacteria, bacillus subtilis, bacillus licheniformis, amino acid, zinc sulfate, calcium chloride, utilizes
Strain adds agricultural crop straw, crude fibre and the production in soil with the substance that agricultural film etc. is difficult to degrade in complex microorganism
The decomposed degradation of speed simultaneously utilizes；Wherein the heat-resisting resistance of bacillus subtilis, bacillus licheniformis is strong, to person poultry harmless, does not pollute
Environment, the speed of growth is fast, is easy to survive in soil surface, can secrete a variety of, a large amount of degrading enzyme and antibiotic, bacteriolyze object
Matter, and it is effectively improved the residual of the nitrate nitrogen in soil, heavy metal and residual old pesticide, purification and rehabilitating soil reduce in soil
The growth of harmful bacteria；Meanwhile there is apparent stimulation to the respiratory intensity in soil；
Further, the use of photosynthetic bacteria can promote the photosynthesis of various strains in composite bacteria agent, provide a large amount of oxygen amount and add
Fast beneficial bacterium breeding；Coarse-fibred photosynthesis in soil is also accelerated simultaneously accelerates decomposed, degradation；
Further, amino acid can change that saliferous in soil is excessively high, alkaline too strong, grogs high degree of dispersion, the reason of soil texture difference
Change character, be decreased obviously the soil weight, soil total void and water-holding capacity increase, and facilitate soil fertilizer water conservation, effective to promote
Into plant root system development；
The content of Trace Elements in Soil can be improved in further zinc sulfate, the ratio of various microelements in balanced soil, in advance
Anti- Trace Elements in Soil it is unbalance；Reduce depauperation caused by crop heavy metal is poisoned；
Further calcium chloride can improve soil property, make the soil restoration vigor that acidification, salinization, base fertilizer are excessive, improve soil fertilizer
Power, the harm of pre- preventing disease and pest, germ improve disease-resistant crops, antibacterial, anti-adversity ability；Pesticide Residue in Soil residual is decomposed, is improved
Adsorption by soil；Improve soil acidity or alkalinity and gas permeability；
Microbial inoculum can improve land structures in this composite bacteria agent, and bulk density reduces, and porosity increases, and enhance soil penetrability, effectively
Improve soil erosion；PH value in balanced soil reduces soil alkaline evil；The microelements such as slow phosphorus, potassium in soil are activated,
Beneficial bacterium content is supplemented, the breeding of harmful bacteria is inhibited, promotes photosynthesis, protects crop root system, promotes well developed root system, enhancing
Resistance；Existing plant disease is eliminated, the generation of plant disease is reduced.
Specific embodiment:
Embodiment below does not limit the present invention convenient for better understanding the present invention.
A kind of rice field complex microbial inoculum includes three kinds of photosynthetic bacteria, bacillus subtilis, bacillus licheniformis bacterium
Kind and three kinds of amino acid, zinc sulfate, calcium chloride compounds；By above-mentioned strain, compound addition dilution pure water, and according to
Composite bacteria agent is made in following mass ratio:
Photosynthetic bacteria 10 ~ 20%,
Bacillus subtilis 10 ~ 15%,
Bacillus licheniformis 5 ~ 8%,
Amino acid 6 ~ 8%,
Zinc sulfate 0.5 ~ 1%,
Calcium chloride 1 ~ 3%,
Pure water 50 ~ 55%；
Wherein, three kinds of photosynthetic bacteria, bacillus subtilis, bacillus licheniformis strains have contained in the composite bacteria agent
Imitate viable count be greater than 200,000,000/liter.
The best proportion of strain, compound and dilution pure water used in composite bacteria agent are as follows: photosynthetic bacteria 15%,
Bacillus subtilis 15%, bacillus licheniformis 7%, amino acid 6%, zinc sulfate 1%, calcium chloride 1%, pure water 55%.
Bacillus licheniformis used is bacillus megaterium, side bacillus mixture.
Amino acid used is glycine, phenylalanine mixture.
In order to make the objectives, technical solutions, and advantages of the present invention clearer, below in conjunction with specific embodiment, to this
Invention is further elaborated.
When in use:
S1, huge gemma bar, side bacillus are obtained respectively, sprinkle liquid medium on its surface, after static 30min, be put into and stir
It mixes and stirs 10min in machine, be uniformly mixed it and intermixture A is made；
In S2, respectively acquirement glycine, the same dosage container of phenylalanine, it is uniformly mixed and intermixture B is made；
S3, by intermixture B made of intermixture A, S2 manufactured in S1, be added photosynthetic bacteria, bacillus subtilis, amino acid,
Zinc sulfate, calcium chloride；Then it puts in a kettle, temperature control is 27 DEG C, and interval 5min is once stirred, and is taken after 25min
Out, mixture is obtained；
S4, room temperature is down in the mixture produced in S3 taking-up, pure water dilution dissolution is added, complex microbial inoculum is made；
Complex microbial inoculum is uniformly sprayed and is sprinkling upon on soil, it is primary to carry out scarifying to soil two days later；
Microbial inoculum and improvement land structures, bulk density reduce in this composite bacteria agent, and porosity increases, and enhances soil penetrability, effectively
Improve soil erosion；PH value in balanced soil reduces soil alkaline evil；The microelements such as slow phosphorus, potassium in soil are activated,
Beneficial bacterium content is supplemented, the breeding of harmful bacteria is inhibited, protects crop root system, eliminates existing plant disease, reduces phytopathy
Harmful generation promotes crop root system absorbability, protects root system, enhances anti-adversity ability.
Claims (5)
1. a kind of rice field complex microbial inoculum, it is characterised in that: include photosynthetic bacteria, bacillus subtilis, lichens gemma
Three kinds of strains of bacillus and three kinds of amino acid, zinc sulfate, calcium chloride compounds；Above-mentioned strain, compound are added and diluted with pure
Water purification, and composite bacteria agent is made according to following mass ratio:
Photosynthetic bacteria 10 ~ 20%,
Bacillus subtilis 10 ~ 15%,
Bacillus licheniformis 5 ~ 8%,
Amino acid 6 ~ 8%,
Zinc sulfate 0.5 ~ 1%,
Calcium chloride 1 ~ 3%,
Pure water 50 ~ 55%；
Wherein, three kinds of photosynthetic bacteria, bacillus subtilis, bacillus licheniformis strains have contained in the composite bacteria agent
Imitate viable count be greater than 200,000,000/liter.
2. a kind of rice field complex microbial inoculum according to claim 1, it is characterised in that: strain used, compound
And best proportion of the dilution pure water in composite bacteria agent are as follows: photosynthetic bacteria 15%, bacillus subtilis 15%, lichens gemma
Bacillus 7%, amino acid 6%, zinc sulfate 1%, calcium chloride 1%, pure water 55%.
3. a kind of rice field complex microbial inoculum according to claim 1, it is characterised in that: photosynthetic bacteria used can be with
It is capsula Rhodopseudomonas, Rhodopseudomonas palustris.
4. a kind of rice field complex microbial inoculum according to claim 1, it is characterised in that: bacillus licheniformis used
It can be bacillus megaterium, side bacillus.
5. a kind of rice field complex microbial inoculum according to claim 1, it is characterised in that: amino acid used can be
Glutamic acid, glycine, threonine, phenylalanine.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811141484.6A CN109234195A (en) | 2018-09-28 | 2018-09-28 | A kind of rice field complex microbial inoculum |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811141484.6A CN109234195A (en) | 2018-09-28 | 2018-09-28 | A kind of rice field complex microbial inoculum |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104591888A (en) * | 2014-12-31 | 2015-05-06 | 新疆光合元生物科技有限公司 | Method for preventing and treating fragrant pear rot, preparation thereof and production method of preparation |
| CN105819943A (en) * | 2016-03-01 | 2016-08-03 | 深圳市芭田生态工程股份有限公司 | Microbial organic fertilizer and preparation method thereof |
| US20160243172A1 (en) * | 2013-02-04 | 2016-08-25 | Seres Therapeutics, Inc. | Compositions and Methods for Inhibition of Pathogenic Bacterial Growth |
| CN107056535A (en) * | 2017-03-31 | 2017-08-18 | 山东宝源生物科技股份有限公司 | A kind of composite microbiological fertilizer and its application |
-
2018
- 2018-09-28 CN CN201811141484.6A patent/CN109234195A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20160243172A1 (en) * | 2013-02-04 | 2016-08-25 | Seres Therapeutics, Inc. | Compositions and Methods for Inhibition of Pathogenic Bacterial Growth |
| CN104591888A (en) * | 2014-12-31 | 2015-05-06 | 新疆光合元生物科技有限公司 | Method for preventing and treating fragrant pear rot, preparation thereof and production method of preparation |
| CN105819943A (en) * | 2016-03-01 | 2016-08-03 | 深圳市芭田生态工程股份有限公司 | Microbial organic fertilizer and preparation method thereof |
| CN107056535A (en) * | 2017-03-31 | 2017-08-18 | 山东宝源生物科技股份有限公司 | A kind of composite microbiological fertilizer and its application |
Non-Patent Citations (4)
| Title |
|---|
| 何若天: "《农用益生菌生产与应用手册》", 29 February 2016, 金盾出版社 * |
| 罗勇胜等: ""光合细菌与芽孢杆菌协同净化养殖水体的研究"", 《农业环境科学学报》 * |
| 肖晶晶等: ""生物强化技术优势及环保菌剂研究应用现状"", 《环境科学导刊》 * |
| 褚天铎等: "《简明施肥技术手册》", 31 January 2014, 金盾出版社 * |
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Application publication date: 20190118 |