CN109231467A - A method of using gas lift circulation algae biofilm reactor for treatment black and odorous water - Google Patents
A method of using gas lift circulation algae biofilm reactor for treatment black and odorous water Download PDFInfo
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 81
- 241000195493 Cryptophyta Species 0.000 title claims abstract description 46
- 238000000034 method Methods 0.000 title claims abstract description 39
- 239000007789 gas Substances 0.000 claims abstract description 28
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 22
- 235000007091 Chlorella pyrenoidosa Nutrition 0.000 claims abstract description 20
- 238000005276 aerator Methods 0.000 claims abstract description 16
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 12
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 12
- 239000011574 phosphorus Substances 0.000 claims abstract description 12
- 239000003344 environmental pollutant Substances 0.000 claims abstract description 11
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 11
- 231100000719 pollutant Toxicity 0.000 claims abstract description 11
- 238000012545 processing Methods 0.000 claims abstract description 6
- 241000195652 Auxenochlorella pyrenoidosa Species 0.000 claims abstract 7
- 238000005273 aeration Methods 0.000 claims description 9
- 238000006243 chemical reaction Methods 0.000 claims description 7
- 238000005286 illumination Methods 0.000 claims description 7
- 239000012528 membrane Substances 0.000 claims description 7
- 230000005540 biological transmission Effects 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims description 3
- 238000004804 winding Methods 0.000 claims description 2
- 239000010865 sewage Substances 0.000 abstract description 14
- 238000003306 harvesting Methods 0.000 abstract description 12
- 230000008901 benefit Effects 0.000 abstract description 10
- 239000002028 Biomass Substances 0.000 description 13
- 244000249214 Chlorella pyrenoidosa Species 0.000 description 13
- 241000195649 Chlorella <Chlorellales> Species 0.000 description 9
- 229910052799 carbon Inorganic materials 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 238000012163 sequencing technique Methods 0.000 description 5
- 238000012546 transfer Methods 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 241000195651 Chlorella sp. Species 0.000 description 3
- YUWBVKYVJWNVLE-UHFFFAOYSA-N [N].[P] Chemical compound [N].[P] YUWBVKYVJWNVLE-UHFFFAOYSA-N 0.000 description 3
- 238000011953 bioanalysis Methods 0.000 description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
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- 230000003321 amplification Effects 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000012851 eutrophication Methods 0.000 description 2
- 239000000945 filler Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 230000000258 photobiological effect Effects 0.000 description 2
- 230000000243 photosynthetic effect Effects 0.000 description 2
- 238000003672 processing method Methods 0.000 description 2
- 239000010802 sludge Substances 0.000 description 2
- 230000001360 synchronised effect Effects 0.000 description 2
- 239000002351 wastewater Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 244000082204 Phyllostachys viridis Species 0.000 description 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000011425 bamboo Substances 0.000 description 1
- 238000006065 biodegradation reaction Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
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- 238000011161 development Methods 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
- C02F3/325—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/105—Phosphorus compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/02—Odour removal or prevention of malodour
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- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Water Supply & Treatment (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Botany (AREA)
- Ecology (AREA)
- Treating Waste Gases (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to technical field of sewage, disclose a kind of method using gas lift circulation algae biofilm reactor for treatment black and odorous water.Chlorella pyrenoidosa is seeded to biofilm culture on biological rope carrier, by black and odorous water out of, water inlet is directed into reactor cylinder, it is then turned on light source and aerator, gas rises through guide shell and reactor is divided into up-flow area and the area Jiang Liu, biological rope carrier is located in up-flow area, chlorella pyrenoidosa on biological rope carrier reacts the nitrogen and phosphorus pollutants in removal black and odorous water by mixotrophic growth, closes light source later and aerator is stood, finally will treated that water is discharged from water outlet.The device of the invention and method have many advantages, such as that operating cost is low, effluent quality is good, algae easily harvests, and have biggish application potential in terms of the processing of black and odorous water.
Description
Technical field
The invention belongs to technical field of sewage, and in particular to a kind of using at gas lift circulation algae bio membrane reactor
The method for managing black and odorous water.
Background technique
In recent years, black smellyization of urban water-body gradually aggravates, and it is extremely urgent to administer black and odorous water.It is formed for black and odorous water
Basic reason --- Water, phosphorus and organic pollutant excessive problem, currently used processing method have physical method, chemical method
And bioanalysis.Bioanalysis is due to having many advantages, such as environmental-friendly, ecological energy-saving, it has also become most potential black and odorous water is controlled
Reason method.Although traditional bioanalysis such as activated sludge process and the new process developed on this basis are to the removal rate energy of COD
Up to 90% or more, but denitrogenation dephosphorizing efficiency is generally lower, it is difficult to agree with the demand of sewage treatment " proposing mark upgrading ".Microalgae has life
Long rate is fast, economic value is high and it is good to nitrogen and phosphorus pollutants removal effect the advantages that, in the improvement field of eutrophication water
With significant advantage.
Domestic and international result of study shows that the chlorella (Chlorella sp.) for being widely used in sewage treatment has growth speed
The features such as rate is fast, strong environmental adaptability can not only utilize CO2Carry out photoautotrophy effect, moreover it is possible to while absorbing organic carbon
Source, inorganic carbon source and luminous energy carry out mixotrophic growth.It is the eucaryotic cell structure of chlorella, intracellular under different condition of culture
Material composition and physiological metabolism mechanism can change.A large number of studies show that with photosynthetic autotrophs Chlorella sp. phase
Than mixotrophism type Chlorella sp. has faster growth rate, and higher optical energy utilization efficiency and stronger nitrogen phosphorus are inhaled
Receipts ability can absorb carbon dioxide and luminous energy in atmosphere in the case where organic carbon source exhausts and persistently remove nitrogen phosphorus in sewage
Pollutant.Li et al. people (Li T., Zheng, Y., Yu, L., &Chen, S., Applied microbiology and
Biotechnology, 2014.66,204-213) et al. in suspension microalgae waste water treatment system, light is carried out to chlorella respectively
Close autotrophy and Combined hardening model, the results showed that under the Combined hardening model mode of addition 4g/L glucose, the ratio of chlorella
Growth rate and maximum biomass are 5.4 and 5.2 times of photoautotrophy mode respectively.Wang et al. (Wang, H., Xiong, H.,
Hui, Z., &Zeng, X., Bioresource Technology, 2012.104,215-220.) with after diluting in various degree
Pig raising wastewater to chlorella pyrenoidosa carry out Combined hardening model, the results showed that COD be 1000mgL-1When, obtain highest
Biomass concentration 0.29gL-1, to the removal rate of COD, TN, TP also highest, respectively 55.4%, 74.6%, 77.7%.Cause
This, mixotrophism type chlorella sewage disposal technology has higher pollutant removal rate and higher Biomass yield.
However, current mixotrophism type chlorella sewage disposal technology mostly uses suspended alga cultivating system, exist out
The problems such as aqueous suspension concentration is high, take up a large area, algae biomass difficulty harvest, most effective solution is algal immobilization skill
Art.On the basis of microalgae immobilization, algae biofilm develops into a kind of new sewage treatment means, which not only reduces
The cost of biofilm carrier, and it is more easy in links such as algae inoculation, culture and harvests.Feng et al. (Gao, F.,
Yang, Z.H., Li, C.et al, Bioresource Technology, 2015.179,8-12) using through chlorella biofilm
Algae photo-biological membrane reactor handles secondary sewage, NH4 +- N, PO4 3-The removal rate of-P respectively reaches 96% and 85.9%, algae
Biomass yield improves 44% compared with suspended alga reactor.
Typical algae bio film system has waterpower algae bed, biological rotating disk, tubulose and flat algae biofilm light anti-
Answer device etc., but it is generally existing take up a large area, the problems such as biomass is unstable, mass-transfer efficiency is low, application can be engineered by developing
High-efficient biological reactor the development of algae sewage disposal technology is had a very important significance.Column gas lift circulation photo-biological
Reactor not only has occupied area small, the high advantage of the efficiency of light energy utilization height and mass-transfer efficiency of algae, moreover it is possible to prevent in waste water
Dissolved oxygen supersaturation problem improves reactor contaminant removal efficiency.The Chinese invention of 203639230 U of Publication No. CN is special
Benefit discloses circulation composite filling bioreactor in a kind of gas-lifting type, which mentions biological potsherd filling and bamboo silk filler
It is filled in the up-flow area and the area Jiang Liu of gas lift circulation flow reactor respectively, there is good place to low concentration, low-carbon, high-ammonia-nitrogen sewage
Manage effect.But playing the main body of biodegradation in the gas-lifting type in circulation filler biological reactor is still activated sludge, instead
It answers device phosphor-removing effect poor, is not able to satisfy the demand of black and odorous water denitrogenation dephosphorizing.
Summary of the invention
In place of the above shortcoming and defect of the existing technology, gas lift is used the purpose of the present invention is to provide a kind of
The method of circulation algae biofilm reactor for treatment black and odorous water.The method of the present invention combination mixotrophism type algae bio membrane technology
It constructs circulation in novel sequential air lifting under the conditions of open culturing with the dual characteristics of column gas lift circulation flow reactor and mixes
Auxotype alga-bacteria biofilm denitrogenation dephosphorizing reaction system carries out depth removal to nitrogen and phosphorus pollutants in black and odorous water, to reach black
The management goal of smelly water body, while harvesting high-biomass microalgae.
The object of the invention is achieved through the following technical solutions:
A method of using gas lift circulation algae biofilm reactor for treatment black and odorous water, the gas lift circulation algae is raw
Object membrane reactor includes light transmission cylinder, and light transmission guide shell is arranged in cylinder, biological rope carrier is arranged in guide shell；Cylinder lower end is set
Cone bottom is set, aerator is arranged in cone bottom center；Simultaneously inlet and outlet are arranged in cylinder outer wall winding white LED lamp band light source；
The method of the processing black and odorous water includes the following steps:
Chlorella pyrenoidosa (Chlorella pyrenoidosa) is seeded to biofilm culture on biological rope carrier, it will be black
Smelly water body is then turned on light source and aerator out of water inlet is directed into reactor cylinder, and gas rises through guide shell
Reactor is divided into up-flow area and the area Jiang Liu, biological rope carrier is located in up-flow area, the chlorella pyrenoidosa on biological rope carrier
The nitrogen and phosphorus pollutants in removal black and odorous water are reacted by mixotrophic growth, light source is closed later and aerator is stood, most
Treated water is discharged from water outlet afterwards.
Further, the chlorella pyrenoidosa being seeded on biological rope carrier passes through additional 10gL-1Glucose
BG11 culture medium carries out sterile preculture and obtains, and the inoculum concentration of chlorella pyrenoidosa is the 20% of reactor effective volume.
Further, the COD concentration of the black and odorous water is 300~400mgL-1, NH4 +- N concentration is 17~20mg
L-1, PO4 3-- P concentration is 8~10mgL-1。
Further, the cylinder, cone bottom and guide shell are made of organic glass, cylinder high 90cm, internal diameter 9cm；It leads
The high 80cm of flow cartridge, diameter 6cm, bottom center 2cm high is bored apart from reactor in guide shell lower end.
Further, the filling rate of the biological rope carrier is the 2/3 of reactor effective volume.Biological rope carrier is organic
With inorganic compounding material, there is close microbes and hydrophily, large specific surface area, biofilm are rapid, the material service life is long, low cost
It is honest and clean.
Further, the column intracorporal time that black and odorous water is directed into reactor from water inlet is 1h, opens light
The time of source and aerator is 16h, time of repose 6h, by treated time that water be discharged from water outlet is 1h, one
The sequencing batch operation period is Light To Dark Ratio 16h:8h for 24 hours, and the aeration quantity of aerator is 0.06Lmin-1, hydraulic detention time
For 4d.
Further, white LED lamp band light source overall length is 10m, power 5.8W, intensity of illumination is 8000~
10000lux。
Further, the temperature of the mixotrophic growth reaction is 25~28 DEG C.
Further, the chlorella pyrenoidosa of biofilm culture was carried out manually every 7 days using sluicing method in reactor
Harvest.
Further, temperature controller is also set up on the reactor and microcomputer control system is used to control the temperature of reactor
Degree, intensity of illumination, Light To Dark Ratio, Inlet and outlet water time, aeration rate and hydraulic detention time parameter.
Method of the invention have the following advantages that and the utility model has the advantages that
(1) mixotrophism type chlorella pyrenoidosa used by the method for the present invention can absorb organic carbon source, inorganic carbon simultaneously
Source and luminous energy are increased, compared with photosynthetic autotrophs chlorella, with faster growth rate, the higher efficiency of light energy utilization
With stronger adaptive capacity to environment, it can continue to absorb carbon dioxide and luminous energy in atmosphere in the case where organic carbon source exhausts and go
Except the nitrogen and phosphorus pollutants in sewage, using mixotrophism type chlorella pyrenoidosa as algae, what is formed under open condition of culture is mixed
Closing auxotype alga-bacteria biofilm has higher pollutant removal rate and higher Biomass yield.
(2) biological rope carrier is set in the up-flow area of reactor by the method for the present invention, is conducive to periphtic algae and is formed algae
Biomembrane solves the problems, such as that algae harvests.
(3) in the method for the present invention treatment process, sewage is tortuous between biological rope carrier under the action of the aerator of bottom
Rise, reach guide shell at the top of when again flow downward from the area Jiang Liu, bottom by again promoted and again with mixotrophism type phycomycete
Biofilm contact reaction, forms inner recirculation flow state.This fluidised form is conducive to strengthen mass transport process, improves reactor mass-transfer efficiency.
(4) the method for the present invention further can control sequencing batch operation mode using temperature controller and microcomputer control system, lead to
The parameters such as temperature, intensity of illumination, Light To Dark Ratio, Inlet and outlet water time, aeration rate and the hydraulic detention time of control reactor are crossed,
Higher micro algae biomass is kept in reactor, realizes black and odorous water synchronous denitrification dephosphorizing, and the microalgae of harvest can carry out recycling
It utilizes.
(5) processing method of the invention has mass-transfer efficiency height, effluent quality good, and microalgae yield is high, and algae easily harvests
Advantage, in terms of the nitrogen and phosphorus pollutants removal of eutrophication water with good application prospect.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of gas lift circulation algae bio membrane reactor in the embodiment of the present invention.
Fig. 2 is the surface scan electron microscope of biomembrane obtained by biofilm culture on biological rope carrier in the embodiment of the present invention.
Fig. 3 is micro- sem observation (2600 times of amplification) figure of the biomass harvested in the embodiment of the present invention.
Fig. 4 and Fig. 5 is respectively nitrogen (NH in the present embodiment black and odorous water4 +- N) and phosphorus (PO4 3-- P) disengaging water concentration and go
Except rate is with days running variation diagram.
Specific embodiment
Present invention will now be described in further detail with reference to the embodiments and the accompanying drawings, but embodiments of the present invention are unlimited
In this.
Embodiment
A kind of method using gas lift circulation algae biofilm reactor for treatment black and odorous water of the present embodiment, the gas lift
The structural schematic diagram of circulation algae bio membrane reactor is as shown in Figure 1.Including light transmission cylinder 1, light transmission guide shell is set in cylinder
2, guide shell is interior to be arranged biological rope carrier 3；Aerator 5 is arranged in cylinder lower end setting cone bottom 4, cone bottom center；Cylinder outer wall twines
Around white LED lamp band light source 6 and water inlet 7 and water outlet 8 are set；The aerator 5 passes through spinner flowmeter 9 and air compressor machine
10 are connected；Also set up on the reactor temperature controller 11 and microcomputer control system 12 control the temperature of reactor, intensity of illumination,
The parameters such as Light To Dark Ratio, Inlet and outlet water time, aeration rate and hydraulic detention time.The cylinder, cone bottom and guide shell are by organic
Glass is made, cylinder high 90cm, internal diameter 9cm；The high 80cm of guide shell, diameter 6cm, bottom center is bored apart from reactor in guide shell lower end
2cm high.The filling rate of the biological rope carrier is the 2/3 of reactor effective volume.White LED lamp band light source overall length is
10m, power 5.8W, intensity of illumination are 8000~10000lux.
The method of the processing black and odorous water includes the following steps:
To pass through additional 10gL-1The mixotrophism type chlorella pyrenoidosa of the sterile preculture of BG11 culture medium of glucose
(Chlorella pyrenoidosa) is algae, and be seeded to biofilm culture, inoculum concentration on biological rope carrier has for reactor
Imitate the 20% of volume.By black and odorous water, (COD concentration is 300~400mgL-1, NH4 +- N concentration is 17~20mgL-1,
PO4 3-- P concentration is 8~10mgL-1) out of, water inlet is directed into reactor cylinder, it is then turned on light source and aerator,
Gas rises through guide shell and reactor is divided into up-flow area and the area Jiang Liu, and biological rope carrier is located in up-flow area, and biological rope carries
Chlorella pyrenoidosa on body reacts the nitrogen and phosphorus pollutants in removal black and odorous water by mixotrophic growth, closes light source later
It stands with aerator, finally treated water is discharged from water outlet.It is anti-using temperature controller and microcomputer control system control
It answers device with sequencing batch operation mode operation, is water inlet 1h the step of sequencing batch operation mode, micro- aeration 16h, stands 6h, be discharged
1h, a batch-type period are for 24 hours.Light source is opened when aeration reaction, closes light source, aeration quantity in water inlet, standing and water outlet
Control is in 0.06Lmin-1Left and right, hydraulic detention time 4d, reaction temperature are controlled at 25~28 DEG C.Utilize mixotrophism type
The features such as alga-bacteria biofilm growth rate is fast, strong environmental adaptability, sequencing batch operation mode are able to maintain higher algae biomass
The characteristic and high advantage of internal airlift loop reactor mass-transfer efficiency is synchronous improves black and odorous water denitrogenation dephosphorizing efficiency, and testing
It carries out 7d manually to harvest alga-bacteria biofilm, the microalgae of harvest can realize resource utilization.
The mixotrophism type chlorella pyrenoidosa of preculture is seeded to biofilm culture institute on biological rope carrier by the present embodiment
The surface scan electron microscope for obtaining biomembrane is as shown in Figure 2.From Figure 2 it can be seen that microorganism is attached in large quantities on biological rope carrier, hang
Film effect is preferable, and biofilm surface is uneven, and cell is directly connected to each other, and forms thicker mixotrophism type phycomycete biology
Film advantageously accounts for algae harvest problem.
When reactor is run to 7d, when there is biomembrane aging phenomenon, using sluicing method in reactor and
Cell on carrier is manually harvested, and micro- sem observation (2600 times of amplification) is carried out to the biomass of harvest, as a result such as Fig. 3 institute
Show.As seen from Figure 3, chlorella is the main bacteria seed in this reactor.It is raw that the algae harvested in reactor is measured by dry weight method
Object amount, algae biomass concentration reach 0.67gL-1, yield 0.096gL-1·d-1.Should the result shows that, using above-mentioned processing work
Higher microalgae productivity can be obtained in skill, realizes that algae resourceization utilizes while improving and polluting removal efficiency.
Nitrogen (NH in the present embodiment black and odorous water4 +- N) and phosphorus (PO4 3-- P) disengaging water concentration and removal rate with days running
Variation is as shown in Figure 4 and Figure 5 respectively.By Fig. 4 and Fig. 5 result it is found that mixotrophism type alga-bacteria biofilm has black and odorous water
Good adaptability reaches moderately good Nitrogen/Phosphorus Removal, NH during stable operation in the 2d of reactor operation4 +-N
And PO4 3-The average removal rate of-P respectively reaches 90.35% and 97.63%, is discharged NH4 +- N and PO4 3-- P concentration can be down to respectively
2.0mg·L-1And 0.2mgL-1Hereinafter, meeting " water environment quality standard " (GB3838-2002) V class water quality standard.Thus
It is found that circulation algae biofilm reactor and its operation method in a kind of gas lift disclosed by the invention, have sewage good
Nitrogen phosphorus ligands effect has biggish application potential in terms of black and odorous water processing.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (10)
1. a kind of method using gas lift circulation algae biofilm reactor for treatment black and odorous water, it is characterised in that: the gas lift
Circulation algae bio membrane reactor includes light transmission cylinder, and light transmission guide shell is arranged in cylinder, biological rope carrier is arranged in guide shell；
Aerator is arranged in cylinder lower end setting cone bottom, cone bottom center；Simultaneously water inlet is arranged in cylinder outer wall winding white LED lamp band light source
And water outlet；The method of the processing black and odorous water includes the following steps:
Chlorella pyrenoidosa is seeded to biofilm culture on biological rope carrier, black and odorous water is directed into reactor from water inlet
In cylinder, it is then turned on light source and aerator, gas rises through guide shell and reactor is divided into up-flow area and the area Jiang Liu, raw
Object rope carrier is located in up-flow area, and the chlorella pyrenoidosa on biological rope carrier removes black smelly water by mixotrophic growth reaction
Nitrogen and phosphorus pollutants in body are closed light source and aerator later and are stood, finally will treated that water is discharged from water outlet.
2. a kind of method using gas lift circulation algae biofilm reactor for treatment black and odorous water according to claim 1,
It is characterized by: the chlorella pyrenoidosa being seeded on biological rope carrier passes through additional 10gL-1The BG11 of glucose is trained
It supports the sterile preculture of base progress to obtain, the inoculum concentration of chlorella pyrenoidosa is the 20% of reactor effective volume.
3. a kind of method using gas lift circulation algae biofilm reactor for treatment black and odorous water according to claim 1,
It is characterized by: the COD concentration of the black and odorous water is 300~400mgL-1, NH4 +- N concentration is 17~20mgL-1,
PO4 3-- P concentration is 8~10mgL-1。
4. a kind of method using gas lift circulation algae biofilm reactor for treatment black and odorous water according to claim 1,
It is characterized by: the cylinder, cone bottom and guide shell are made of organic glass, cylinder high 90cm, internal diameter 9cm；Guide shell is high
Bottom center 2cm high is bored apart from reactor in 80cm, diameter 6cm, guide shell lower end.
5. a kind of method using gas lift circulation algae biofilm reactor for treatment black and odorous water according to claim 1,
It is characterized by: the filling rate of the biological rope carrier is the 2/3 of reactor effective volume.
6. a kind of method using gas lift circulation algae biofilm reactor for treatment black and odorous water according to claim 1,
It is characterized by: the column intracorporal time that black and odorous water is directed into reactor from water inlet is 1h, light source and exposure are opened
The time of device of air is 16h, time of repose 6h, and by treated, time that water be discharged from water outlet is 1h, a batch-type
The cycle of operation is Light To Dark Ratio 16h:8h for 24 hours, and the aeration quantity of aerator is 0.06Lmin-1, hydraulic detention time 4d.
7. a kind of method using gas lift circulation algae biofilm reactor for treatment black and odorous water according to claim 1,
It is characterized by: the white LED lamp band light source overall length is 10m, power 5.8W, intensity of illumination is 8000~10000lux.
8. a kind of method using gas lift circulation algae biofilm reactor for treatment black and odorous water according to claim 1,
It is characterized by: the temperature of the mixotrophic growth reaction is 25~28 DEG C.
9. a kind of method using gas lift circulation algae biofilm reactor for treatment black and odorous water according to claim 1,
It is characterized by: the chlorella pyrenoidosa of biofilm culture was manually harvested every 7 days using sluicing method in reactor.
10. a kind of method using gas lift circulation algae biofilm reactor for treatment black and odorous water according to claim 1,
It is characterized by: also setting up temperature controller and microcomputer control system on the reactor for controlling temperature, the illumination of reactor
Intensity, Light To Dark Ratio, Inlet and outlet water time, aeration rate and hydraulic detention time parameter.
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| CN110395848A (en) * | 2019-08-05 | 2019-11-01 | 西北农林科技大学 | A method of black and odorous water is administered using microalgae |
| CN110723823A (en) * | 2019-10-25 | 2020-01-24 | 山东理工大学 | Device for jointly treating black and odorous water body by utilizing contact oxidation and microalgae |
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| CN112624527A (en) * | 2020-12-31 | 2021-04-09 | 哈尔滨工程大学烟台研究院 | Algae-membrane biological composite sewage treatment device |
| CN112919641A (en) * | 2021-03-23 | 2021-06-08 | 南京科盛环保技术有限公司 | Method for removing nitrogen and phosphorus by using microalgae and treatment device |
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| CN110723871A (en) * | 2019-10-29 | 2020-01-24 | 山东理工大学 | Method for treating black and odorous water by combining indigenous microbial film and microalgae |
| CN110723871B (en) * | 2019-10-29 | 2022-04-22 | 山东理工大学 | Method for treating black and odorous water by combining indigenous microbial film and microalgae |
| CN111533263A (en) * | 2020-05-12 | 2020-08-14 | 浙江伊诺环保科技股份有限公司 | Movable microecological treatment device and method for nitrogen and phosphorus removal of wastewater |
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| CN112624527A (en) * | 2020-12-31 | 2021-04-09 | 哈尔滨工程大学烟台研究院 | Algae-membrane biological composite sewage treatment device |
| CN112919641A (en) * | 2021-03-23 | 2021-06-08 | 南京科盛环保技术有限公司 | Method for removing nitrogen and phosphorus by using microalgae and treatment device |
| CN112919641B (en) * | 2021-03-23 | 2024-01-16 | 南京科盛环保技术有限公司 | Method and treatment device for denitrification and dephosphorization by utilizing microalgae |
| CN113201439A (en) * | 2021-05-17 | 2021-08-03 | 江苏科技大学 | Chlorella culture device coupled with silk reeling wastewater treatment and culture method thereof |
| CN113248021A (en) * | 2021-05-17 | 2021-08-13 | 哈尔滨工业大学 | Method for treating rural domestic sewage by using chlorella pyrenoidosa |
| CN115784419A (en) * | 2022-12-13 | 2023-03-14 | 山东大学 | Organic carbon source supply method and system based on microalgae culture |
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