CN112390381A - Device for quickly film-forming artificial aquatic weeds - Google Patents
Device for quickly film-forming artificial aquatic weeds Download PDFInfo
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- CN112390381A CN112390381A CN202011393058.9A CN202011393058A CN112390381A CN 112390381 A CN112390381 A CN 112390381A CN 202011393058 A CN202011393058 A CN 202011393058A CN 112390381 A CN112390381 A CN 112390381A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
- C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
- C12N11/084—Polymers containing vinyl alcohol units
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/007—Contaminated open waterways, rivers, lakes or ponds
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/004—Apparatus and plants for the biological treatment of water, waste water or sewage comprising a selector reactor for promoting floc-forming or other bacteria
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/006—Apparatus and plants for the biological treatment of water, waste water or sewage details of construction, e.g. specially adapted seals, modules, connections
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/15—N03-N
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/14—Maintenance of water treatment installations
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Abstract
The invention discloses a device for quickly hanging a membrane on artificial aquatic weeds, which relates to the technical field of river water purification and comprises an artificial aquatic weed support frame, wherein a biological rope I and a provenance biological rope are respectively hung on the artificial aquatic weed support frame, the provenance biological rope consists of a biological rope II and immobilized provenance small ball bags, the surfaces of the biological rope II and the immobilized provenance small ball bags are hung at intervals along the length extension direction, each immobilized provenance small ball bag comprises a net bag, immobilized small balls are arranged in the net bag, and the bag opening of the net bag is tied through a hanging rope and hung on the biological rope II. According to the invention, the immobilized pellet is prepared by arranging the provenance biological rope, the immobilized provenance pellet bag and the immobilized pellet and adopting a composite immobilization method of SA, SA and PVA, the prepared immobilized provenance can keep the stability of the strain activity for a long time, and the problems of low natural film-forming speed, low content of functional microorganisms and easy loss of exogenously added microbial agents in a river channel are solved.
Description
Technical Field
The invention relates to the technical field of riverway water quality purification, in particular to a device for quickly hanging a membrane on artificial aquatic plants.
Background
Biological denitrification and COD (chemical oxygen demand) degradation are the most efficient, economical, clean methods of treating wastewater. However, in practical application, the free bacteria have certain limitations, such as poor resistance to adverse environments, and unobvious denitrification effect when the biomass of the strain is low. The immobilized microorganism technology is to immobilize free microorganisms having specific physiological functions inside or on the surface of a carrier material by physical or chemical means, and to effectively utilize the immobilized microorganisms. The technology has the advantages of high microbial activity, high microbial density in unit space, good tolerance, strong impact load resistance, high treatment efficiency and the like, and is widely applied to the field of wastewater treatment at present.
The biomembrane process is a method for immobilizing microorganisms, and is characterized in that a large amount of bacteria, fungi, algae and other microorganisms attached to a carrier material are used for removing pollutants in a water body, and the method has the characteristics of impact load resistance, small sludge production amount and the like. The removal rate of the biological filler on suspended substances in the water body can reach 90 percent, and the removal rate of TN (total nitrogen) is also 46 percent. However, when the biological filler is applied to a river water body, the natural biofilm with the pollutant removing performance of the biological filler is mainly formed by the natural adhesion and growth of microorganisms in the water body, the required time is long, and about 50 days is usually required. In addition, in the natural biofilm formation process, the selectivity of microorganisms is poor, and the formed microbial membrane still needs to be domesticated for a long time to play a good denitrification function. River water has the characteristics of an open system and high fluidity, and can cause the exogenous addition of functional microbial bacteria liquid to be easily lost, so that the exogenous direct addition of the functional microbial bacteria liquid is not favorable for promoting the rapid formation of a functional microbial film.
Aiming at the problems that the natural biofilm formation speed is low, the content of functional microorganisms is low, and exogenously added microbial agents are easy to run off in a river channel, the device for the artificial aquatic plant rapid biofilm formation in the river channel is provided, and the device still has a good low-temperature denitrification function even in winter, so that the water quality of the river channel is rapidly improved.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a device for quickly biofilm culturing of artificial aquatic plants, and solves the problems of low natural biofilm culturing speed, low content of functional microorganisms and easy loss of exogenously added microbial agents in a river channel.
(II) technical scheme
In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a device that is used for quick biofilm culturing of artifical pasture and water, includes artifical pasture and water braced frame, hang biological rope one and the biological rope of provenance on the artifical pasture and water braced frame respectively, the biological rope of provenance comprises two and its surperficial immobilized provenance ball bags that hang along length extending direction interval of biological rope, immobilized provenance ball bag includes the pocket, the immobilized bobble is equipped with in the pocket, the sack of pocket is pricked mouthful and is hung on two biological ropes through hanging the rope.
Preferably, the immobilized pellet is synthesized by uniformly mixing the bacterial suspension and the immobilized carrier, then dropwise adding the mixture into a cross-linking agent solution, and carrying out cross-linking at the temperature of 4 ℃.
Preferably, the fixing carrier consists of 4% SA, and the cross-linking agent solution consists of 3% CaCl2The solution is prepared by uniformly dripping mixed solution of bacterial suspension and 4% SA into 3% CaCl2And placing the solution in the solution, wherein the immobilized beads synthesized by crosslinking at the temperature of 4 ℃ account for half of the immobilized beads filled in the mesh bag, and SA is sodium alginate.
Preferably, the fixing carrier consists of 10% PVA + 1% SA, and the cross-linking agent solution consists of 1% CaCl2The saturated boric acid solution is prepared by uniformly dripping mixed solution of bacterial suspension and 10% PVA + 1% SA into solution containing 1% CaCl2The immobilized beads synthesized by cross-linking at 4 ℃ in the saturated boric acid solution account for half of the immobilized beads filled in the mesh bag, and PVA (polyvinyl alcohol) is used.
Preferably, the immobilized bead has a diameter of 0.5 cm.
Preferably, the mesh bag is a 100-mesh nylon mesh.
Preferably, the mesh bag is filled with 1-2g of immobilized pellets.
Preferably, the immobilized seed pellet bags are uniformly fixed on the biological rope II of 50-100cm at intervals of 10-15 cm.
Preferably, the filler of the first biological rope and the second biological rope are annular in shape and made of fine fiber filaments.
(III) advantageous effects
The invention provides a device for quickly filming artificial aquatic weeds, which has the following beneficial effects:
(1) according to the invention, through arranging the provenance biological rope, the immobilized provenance small ball bag and the immobilized small ball, the immobilized small ball is prepared by adopting a composite immobilization method of SA, SA and PVA, and then through freeze drying, the prepared immobilized provenance can keep the stability of the activity of the strain for a long time, and the requirements of use and transportation are met; in use, the SA immobilized beads are gradually dissolved in a water body, and a large amount of bacteria are released into the water body for early rapid biofilm formation; the SA + PVA composite immobilized pellet can maintain stable form, continuously provides a seed source for film formation of artificial aquatic plants, has a good low-temperature denitrification function even in winter, and quickly improves the water quality of a river channel.
(2) The fixed bacteria liquid can be flexibly selected, and can be self-cultured bacteria liquid, purchased commercial bacteria culture liquid, commercial bacteria powder and the like; the prepared seed source biological rope can be combined with different seed source small balls to carry out combined film hanging according to requirements, so that the processing capacity is improved, and the seed source biological rope is convenient to install and replace.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a partial structure diagram of the provenance rope according to the present invention;
FIG. 3 is a schematic view of the structure of the immobilized seed-bag of the present invention;
FIG. 4 is a comparison line graph of denitrification capability of the seed source biological rope and the free bacteria as the artificial aquatic weed inoculation source under the condition of 20 ℃ in the invention;
FIG. 5 shows the seed biological rope as the artificial aquatic weed inoculation source NO at 10 deg.C3 --N and NO2 --a time-dependent profile of the N concentration.
In the figure: 1. an artificial aquatic plant support frame; 2. a biological rope I; 3. a provenance rope; 31. a second biological rope; 32. a fixed seed-source small ball bag; 321. a mesh bag; 322. immobilizing the beads; 323. and (7) hanging a rope.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1 to 5, the present invention provides a technical solution: a device for membrane is hung fast to artifical pasture and water, includes artifical pasture and water braced frame 1, has hung biological rope 2 and provenance biological rope 3 on artifical pasture and water braced frame 1 respectively, also can hang through the heavy object mode of weighing down, every 1m3The artificial aquatic weed is placed with 1 provenance biological rope 3 and is completely immersed in water for membrane hanging, the provenance biological rope 3 consists of a biological rope II 31 and immobilized provenance small ball bags 32, the surfaces of the biological rope II 31 and the immobilized provenance small ball bags 32 are hung at intervals along the length extension direction, each immobilized provenance small ball bag 32 comprises a net bag 321, an immobilized small ball 322 is installed in each net bag 321, and the opening of each net bag 321 is tied through a hanging rope 323 and hung on the biological rope II 31.
As a technical optimization scheme of the invention, the immobilized pellet 322 is prepared by uniformly mixing the bacterial suspension and the immobilized carrier, then dropwise adding the mixture into a cross-linking agent solution, and carrying out cross-linking synthesis at the temperature of 4 ℃.
As a technical optimization scheme of the invention, the fixed carrier consists of 4 percent of SA, and the cross-linking agent solution consists of 3 percent of CaCl2The solution composition is that the bacterial suspension is evenly mixed with 4 percent SA, and the mixed solution is slowly dripped into 3 percent CaCl by a sterile syringe2Adding into the solution, cross-linking in a refrigerator at 4 deg.C for 24 hr, freeze drying the immobilized pellet for long term storage to maintain activity, dissolving the immobilized pellet 322 in water gradually, and adding the mixture of bacterial suspension and 4% SA to 3%CaCl2The immobilized beads 322, which are synthesized by crosslinking and placed in the solution at a temperature of 4 ℃, account for half of the immobilized beads 322 contained in the mesh bag 321.
As a technical optimization scheme of the invention, the fixed carrier consists of 10 percent of PVA and 1 percent of SA, and the cross-linking agent solution contains 1 percent of CaCl2The bacterial suspension is evenly mixed with 10 percent of PVA and 1 percent of SA, and the mixed solution is slowly dripped into the solution containing 1 percent of CaCl by a sterile syringe2The immobilized globules 322 prepared by the method for preparing the immobilized globules of the SA + PVA composite embedding material can maintain stable form, and simultaneously continuously provide seed sources for the film formation of the artificial aquatic weeds, and the mixed solution of the bacterial suspension and 10 percent of PVA +1 percent of SA is uniformly dripped into the mixed solution containing 1 percent of CaCl2And the immobilized beads 322 cross-linked and synthesized at a temperature of 4 c in the saturated boric acid solution of (a) account for half of the immobilized beads 322 contained in the mesh bag 321.
As a technical optimization scheme of the invention, the diameter of the immobilized bead 322 is 0.5 cm.
As a technical optimization scheme of the invention, the mesh bag 321 is a 100-mesh nylon mesh.
As a technical optimization scheme of the invention, 1-2g of immobilized pellets 322 are filled in the mesh bag 321.
As a technical optimization scheme of the invention, the immobilized seed-source small ball bags 32 are uniformly fixed on the biological rope II 31 of 50-100cm at intervals of 10-15 cm.
As a technical optimization scheme of the invention, the fillers of the first biological rope 2 and the second biological rope 31 are annular in shape and made of fine fiber yarns, the external diameter of the biological rope is 45mm, and the specific surface area is 1.6 square meters per m3The porosity is greater than 99%, and the thread mass is 29 g/m.
The river water body is used for simulating low-pollution water to perform sequencing batch type small test, so that the actual environment water body can be well simulated, and the method has representative and actual application values.
TABLE 1 basic river Water quality index
Wherein FIG. 4 shows NO after 12h of the provenance rope group at 20 ℃ compared with the artificial pasture and water group without the provenance rope3 -the-N has 87.5 percent of removal rate, and the removal rate in 24 hours is 98.7 percent; and the removal rate of the artificial aquatic weed group added with the free bacteria as the seed source is less than 35% in 12h, and only 44.1% in 24h can be removed.
FIG. 5 is a monitoring result of the device running for 54 days, wherein the temperature of the device does not exceed 13 ℃ in the whole running process, and the average air temperature is 9.8 ℃; about 50% of NO is present 3 days before start3 -The removal rate of-N is about 1mg/L of NO2 --N accumulation; after 9 days of biofilm formation, NO3 -The removal rate of-N is more than 95 percent, and almost NO NO is generated2 --N accumulation; NO after 15 days3 -The removal rate of-N was almost 100%, and almost NO NO was found2 --accumulation of N; after the device is operated for 48 days, the temperature suddenly drops by 4 ℃, but the removal rate of the nitrate nitrogen reaches 100% within 12 hours.
The working principle is as follows: when the culture medium is used, firstly, culture of denitrifying strains is carried out, a strain N3 stored at the temperature of minus 80 ℃ is taken and streaked on a denitrifying solid culture medium, a single colony is selected and transferred into 3ml of denitrifying liquid culture medium, constant temperature shaking culture is carried out for 12h at the temperature of 30 ℃ and the speed of 160rpm, 1 percent of inoculum concentration is inoculated into 100ml of denitrifying liquid culture medium, culture is carried out for 12h at the temperature of 30 ℃ and the speed of 160rpm, 100ml of culture medium is centrifuged for 5min at the temperature of 4 ℃ and the speed of 10000rpm, supernatant is discarded, physiological saline is used for washing for 3 times, then 10ml of physiological saline is used for resuspending thalli, and then freeze-dried strain immobilized spheres are prepared, and the preparation method of the SA immobilized spheres comprises the following: mixing the bacterial suspension with 4% SA, and slowly adding the mixture into 3% CaCl with sterile syringe2Putting the solution in a refrigerator at 4 ℃ for crosslinking for 24 hours; the preparation method of the SA + PVA composite embedding material immobilized pellet comprises the following steps: uniformly mixing the bacterial suspension with 10% PVA + 1% SA, and slowly dripping the mixed solution into the solution containing 1% CaCl by using a sterile syringe2Then placing the mixture in a refrigerator at 4 ℃ for crosslinking for 24 hours; two groups are mixedImmobilized beads 322 prepared by the method were as follows 1: 1, putting the mixture into a 100-mesh nylon net, putting 1-2g of immobilized small balls 322 into each net bag 321, tying the mixture by a hanging rope 323, hanging the mixture on a second biological rope 31, and then uniformly fixing the immobilized small seed bags 32 on the second biological rope 31 of 50-100cm at intervals of 10-15cm, wherein the biological rope can be used as a biological seed rope 3, each biological seed rope 3 weighs down from top to bottom, or is arranged on an artificial aquatic plant supporting frame 1, and each 1m of the biological seed bags is3The artificial aquatic weed is placed with 1 seed biological rope 3 of 100cm, and is completely immersed in water for biofilm formation.
In conclusion, the invention solves the problems of slow natural membrane hanging speed, low content of functional microorganisms and easy loss of exogenously added microbial agents in the river by arranging the provenance biological rope 3, the immobilized provenance small ball bag 32 and the immobilized small ball 322.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (9)
1. The utility model provides a device that is used for quick biofilm culturing of artifical pasture and water, includes artifical pasture and water braced frame (1), its characterized in that: hang biological rope (2) and biological rope of provenance (3) on artifical pasture and water braced frame (1) respectively, biological rope of provenance (3) comprises biological two (31) of restricting and its surface along the immobilized provenance ball bag (32) that length extending direction interval hung, immobilized provenance ball bag (32) include pocket (321), immobilized ball (322) are equipped with in pocket (321), the sack of pocket (321) is pricked mouthful and is hung on biological two (31) of restricting through string (323).
2. The device for the artificial aquatic weed to quickly form a film according to claim 1, wherein: the immobilized pellet (322) is synthesized by uniformly mixing a bacterial suspension and a fixed carrier, then dripping the mixture into a cross-linking agent solution, and placing the mixture at the temperature of 4 ℃ for cross-linking.
3. The device for the artificial aquatic weed fast film hanging according to the claim 2, characterized in that: the fixed carrier is composed of 4% of SA, and the cross-linking agent solution is composed of CaCl containing 3%2The solution is prepared by uniformly dripping mixed solution of bacterial suspension and 4% SA into 3% CaCl2The immobilized beads (322) which are synthesized by crosslinking in the solution at the temperature of 4 ℃ account for half of the immobilized beads (322) filled in the mesh bag (321).
4. The device for the artificial aquatic weed fast film hanging according to the claim 2, characterized in that: the fixed carrier consists of 10% PVA + 1% SA, and the cross-linking agent solution consists of 1% CaCl2The saturated boric acid solution is prepared by uniformly dripping mixed solution of bacterial suspension and 10% PVA + 1% SA into solution containing 1% CaCl2And the immobilized beads (322) cross-linked and synthesized at the temperature of 4 ℃ in the saturated boric acid solution account for half of the immobilized beads (322) filled in the mesh bag (321).
5. The device for the artificial aquatic weed to quickly form a film according to claim 1, wherein: the diameter of the immobilized bead (322) is 0.5 cm.
6. The device for the artificial aquatic weed to quickly form a film according to claim 1, wherein: the mesh bag (321) is a nylon mesh with 100 meshes.
7. The device for the artificial aquatic weed to quickly form a film according to claim 1, wherein: the mesh bag (321) is internally provided with 1-2g of immobilized pellets (322).
8. The device for the artificial aquatic weed to quickly form a film according to claim 1, wherein: the fixed seed-source small ball bags (32) are uniformly fixed on the biological rope II (31) of 50-100cm at intervals of 10-15 cm.
9. The device for the artificial aquatic weed to quickly form a film according to claim 1, wherein: the filler of the biological rope I (2) and the biological rope II (31) are annular in shape and made of fine fiber filaments.
Priority Applications (1)
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