CN109223885B - Anti-fatigue medicinal liquor - Google Patents

Abstract

The invention relates to an anti-fatigue medicinal liquor, which consists of edible rice wine and effective components; the effective components in each liter of the medicinal liquor are prepared from the following raw material medicines in parts by weight: 45-55 g of mangosteen, 8-12 g of beautiful millettia root, 8-12 g of radix fici simplicissimae, 1.5-2.5 g of dried orange peel and 1.5-2.5 g of liquorice. The medicinal liquor provided by the invention is few in medicinal flavor, and has a remarkable anti-fatigue effect.

Description

Anti-fatigue medicinal liquor

Technical Field

The invention relates to a medical preparation containing unidentified structures of fruits of myrtle, a myrtaceae plant, in particular to medicinal liquor, and the medicinal liquor has an anti-fatigue effect.

Background

Fatigue, a common physical phenomenon in daily life, is not considered to cause serious physical diseases, and is therefore often overlooked by people. However, in ancient Chinese medical literature, there are a lot of discussions about fatigue, and traditional Chinese medicine considers that the occurrence of fatigue phenomena is closely related to the disorder of five internal organs, such as weakness of limbs is mostly related to spleen and stomach, soreness of waist and legs is mostly related to kidney, deficiency of strength is mostly related to lung, unclear brain is mostly related to heart, and intolerance of fatigue is mostly related to liver. Over time, the main manifestations of human behavior include attention-deficit, memory loss, decreased training performance, poor appetite, weight loss, sleepiness and insomnia, dizziness, and prolonged periods of time can cause neurasthenia, fast heart rate at rest, even enlarged heart, pathological noise, etc. Therefore, it is an objective need to find an anti-fatigue method which can exert the characteristics of traditional Chinese medicine and is popular. The traditional Chinese medicine needs to be administered by people, is bitter in taste and difficult to be taken, and the decoction needs to be decocted, so that the operation is complex, and time and labor are wasted. The medicinal liquor is an important health-care product in China, and has good medicinal and physical therapy values.

Disclosure of Invention

The invention aims to solve the technical problem of providing the anti-fatigue medicinal liquor which has less medicinal odor and obvious anti-fatigue effect.

The technical scheme for solving the problems is as follows:

an antifatigue medicated liquor comprises edible rice wine and effective components; the effective components in each liter of the medicinal liquor are prepared from the following raw material medicines in parts by weight: 45-55 g of mangosteen, 8-12 g of beautiful millettia root, 8-12 g of radix fici simplicissimae, 1.5-2.5 g of dried orange peel and 1.5-2.5 g of liquorice.

According to the scheme, the optimal mixture ratio of the raw material medicines is as follows: 50g of mangosteen, 10g of beautiful millettia root, 10g of radix fici simplicissimae, 2g of dried orange peel and 2g of liquorice.

In the scheme, the mangosteen is a fresh and mature mangosteen fruit.

In the scheme, the alcoholic strength of the edible rice wine is 30-50% (namely the volume concentration of the alcohol at 20 degrees is 30-50%).

The medicinal liquor is prepared by the following method:

(1) taking the raw materials, adding 8-10 times of edible rice wine, soaking for 15-60 days, filtering and removing residues to obtain a medicinal liquor stock solution;

(2) adding a proper amount of trehalose into the prepared medicinal liquor stock solution, uniformly stirring, and then adding edible rice wine to a constant volume of 1000ml to obtain the medicinal liquor.

In order to improve the clarity of the medicinal liquor, the alcohol content of the edible rice wine for soaking in the step (1) of the method is the same as that of the edible rice wine for volume determination in the step (2) (namely, the same manufacturer, the same variety and the same alcohol content).

In the formula of the medicinal liquor, the mangosteen is sweet and smooth, enters liver, spleen and lung channels, has the effects of tonifying qi and yin, astringing intestines to arrest spontaneous emission, and nourishing blood and stopping bleeding, and is a monarch drug; the radix millettiae speciosae is sweet and mild, enters lung and kidney channels, tonifies deficiency, moistens lung, strengthens tendons and activates collaterals, has mild nature and sweet and pungent taste, has the functions of tonifying spleen and lung, removing dampness and relaxing tendons, is used as a ministerial drug, and assists the mangosteen to tonify the essence of spleen and lung and benefit the essence of kidney channels; the dried orange peel is used as an adjuvant, the dried orange peel is bitter and warm, the qi is regulated, the spleen is strengthened, the dampness and phlegm are eliminated, and the excessive greasy taste of the medicines is prevented; the liquorice is sweet in nature and has no toxicity, and is used as a messenger drug for harmonizing the effects of the other drugs; the medicines are combined to supplement qi and nourish yin, nourish liver and kidney, invigorate spleen and reduce phlegm.

In order to better understand the present invention, the anti-fatigue and anti-oxidation effects of the medicated wine of the present invention are verified below.

1. Animal experiment method for anti-fatigue efficacy

1.1. Grouping animals

SPF-grade KM mice, 88, weighing 16-18g, male, were randomly divided into two major groups, the first group (used only to determine the time for exhaustive swimming) being divided into model group (Mod.), positive group (Spi.), experimental group 1, experimental group 2 and experimental group 3, for a total of 5 groups of 8 mice each; the second group (used to collect body weight data, anti-fatigue and anti-oxidant data) was divided into blank (Con.), model, positive, experimental groups 1, 2 and 3, for 6 groups, 8 per group. The experiment was performed after one week of acclimatization. Animals in each group were allowed free access to water and food during gavage.

1.2. Animal administration method

The blank group and the model group were given physiological saline, the positive group was given 800mg/kg spirulina, and the experimental group 1, the experimental group 2, and the experimental group 3 were given the medicated liquors of the following examples 1, 2, and 3, respectively. Each group of mice was gavaged 1 time a day with a gavage needle at a dose of 0.1mL/10g (mouse weight) for 20 consecutive days. The body weights of each mouse in the second group were also recorded at the first and last gavage.

1.3. Weight bearing swimming experiment

After the first group of mice was administered for 30min, a weight-bearing swimming test was performed, a lead wire whose weight was 5% of the weight of the mice was loaded on the tail root of the mice, the mice were placed in a swimming tank at a water temperature of (25.0 + -0.5) ° c to a water depth of 40cm, and the time from the start of swimming to exhaustion (the mice still did not float out of the water after sinking for 10 s) was recorded as the mouse exhausted swimming time using a stopwatch.

1.4. Biochemical index detection

The second group of mice was subjected to a weight swimming test 30min after the last administration. The blank group did not swim, and the other five groups were immediately taken out and wiped dry after swimming for 30min under the above-mentioned swimming condition of 1.3. for orbital bleeding, centrifuged at 3000 rpm for 15 min at 4 ℃ and supernatant serum was taken and stored at-80 ℃ for determination of the contents of blood glucose (Glu), urea nitrogen (Bun), lactic acid (LD), Lactate Dehydrogenase (LDH) and Creatine Kinase (CK); taking the blood of the mouse, dislocating and killing the vertebra, immediately taking out the hind leg muscle and the liver, using a part of the hind leg muscle and the liver for measuring Malondialdehyde (MDA), superoxide dismutase (SOD), Catalase (CAT) and glutathione enzyme (GSH), and storing the rest at-80 ℃ for detecting the content of liver glycogen and muscle glycogen.

1.5. Statistical analysis

The data of each group of experiments are expressed as mean plus or minus standard deviation (mean plus or minus SD), Graphpad Prism 7 software is used for drawing, SPSS21.0 software is used for statistical analysis, and a one-factor variance analysis method is adopted, wherein P <0.05 and P <0.01 show that the difference of the two groups has statistical significance.

2. Experimental results of anti-fatigue efficacy

2.1. Influence of the medicinal liquor on the weight of a mouse

After 20 days of administration, each group of mice was active, had good mental status, had smooth skin and hair, and had no adverse reaction. The effect on body weight of the second group of mice is shown in table 1 below. The weight difference of mice in each drug group from the beginning to the end of the experiment has no statistical significance (P is more than 0.05) compared with the blank group, which indicates that the medicinal liquor has no obvious influence on the weight growth of the mice, and indicates that the anti-fatigue effect is not achieved by the weight increasing mode.

TABLE 1 Effect of the medicated wine of the present invention on the body weight of mice

2.2. Influence of the medicinal liquor on exhaustive swimming time of mice

The weight swimming time is the degree of reflecting sports fatigue, in the experiment, the first group of KM mice are taken as research objects, the weight swimming test is carried out after the medicinal liquor disclosed by the invention is continuously perfused for 20 days, and the test result is shown in figure 1. The results show that the mice exhausted swimming time (P <0.01) can be prolonged after taking the positive medicine spirulina and the medicine wine of the invention compared with the model group, wherein the effect of the medicine wine experimental group 1 of the invention is the best. The medicinal liquor provided by the invention can obviously improve the exercise endurance of mice and has an anti-fatigue effect.

2.3. Influence of the medicinal liquor on blood sugar (Glu) of swimming mice

The influence of the medicinal liquor on Glu in mouse serum is shown in figure 2, and compared with a blank group, the medicinal liquor shows that mice in each group are reduced to different degrees after swimming. The difference between the model group and the blank group is significant (P <0.01), and the swimming is shown to reduce the blood sugar content of the mice. Compared with the model group, the spirulina in the positive group obviously improves Glu in the swimming mouse, and the content of Glu in the medicinal liquor dosage group is obviously higher than that in the model group, so that the medicinal liquor dosage group has difference (P is less than 0.01), which shows that the medicinal liquor can improve and slow the consumption of blood sugar in the swimming process and increase the adaptability to organisms.

2.4. The influence of the medicinal liquor on the urinary nitrogen (Bun), lactic acid (LD), Lactate Dehydrogenase (LDH) and Creatine Kinase (CK) in serum

As shown in FIG. 3, the serum levels of Bun, LD, LDH and CK in the model group mice were all significantly increased after swimming (P <0.01) compared with those in the blank group. Compared with a model group, the content of the above 4 indexes (P <0.01) of the positive control group fed with spirulina can be obviously reduced, and different experimental groups of the medicinal liquor can also reduce the content of the indexes, and the medicinal liquor has difference (P <0.05), and particularly the experimental group 1 is optimal. The medicinal liquor can reduce the contents of urea nitrogen, lactic acid, lactate dehydrogenase and creatine kinase in the exercise process and increase the adaptability to the body.

2.5. The medicinal liquor has an antioxidant effect on swimming mice in vivo

In order to evaluate the anti-oxidation effect of the medicinal liquor on mice, the activities of liver SOD, CAT and GSH and the MDA content are measured, the measurement result is shown in figure 4, after the mice in the model group swim for 30min, compared with the mice in the blank group, the MDA content is obviously increased (P <0.01), and the contents of SOD, CAT and GSH are obviously reduced (P < 0.01). However, after the spirulina and the medicinal liquor are orally taken and subjected to swimming experiments, compared with a model group, the spirulina positive group can obviously reduce the content of MDA (P <0.01) and improve the content of SOD, CAT and GSH (P <0.01), the medicinal liquor disclosed by the invention can obviously reduce the content of MDA in the liver of a mouse after being perfused with stomach, and can also obviously improve the content of SOD, CAT and GSH (P <0.05)

2.6. Influence of the medicinal liquor on glycogen concentration of mice

Liver and muscle are the main sites for energy storage in the body, and glycogen is a direct energy source. Experimental data show (figure 5) that liver glycogen and muscle glycogen levels of mice were significantly reduced after swimming (P <0.01) compared to the blank group. Compared with a model group, the spirulina positive group can obviously improve the liver glycogen and the myoglycogen level of mice (P <0.01), and the liver glycogen and the myoglycogen of the mice of each experimental group of the medicinal liquor disclosed by the invention are obviously increased (P <0.05) and are positively correlated with the dosage. The medicinal liquor can reduce the consumption of muscle glycogen and liver glycogen.

Drawings

Fig. 1 is a bar graph of the effect of the medicated wine of the present invention on the time of weight-bearing swimming of mice (n-8), wherein P is <0.01 compared to mod.

Fig. 2 is a graph showing the results of the effect of the medicated wine of the present invention on the blood glucose level of mice (n 8), wherein, # # is P <0.01 compared to con. group and x is P <0.01 compared to mod. group.

Fig. 3 is a graph showing the results of the effect (n 8) of the medicated wine on the contents of mouse urea nitrogen (Bun), Lactate (LD), Lactate Dehydrogenase (LDH) and Creatine Kinase (CK) of the medicated wine of the present invention, wherein a is a bar graph of the mouse Bun content, B is a bar graph of the mouse LD content, C is a bar graph of the mouse LDH content, and D is a bar graph of the mouse CK content. In the figure, # # is P <0.01 compared to con. group, P <0.05 compared to mod. group, and P <0.01 compared to mod. group.

Fig. 4 is a graph showing the results of the effect of the medicated wine of the present invention on the antioxidant effect of mice (n ═ 8), wherein a is a bar graph of the Malondialdehyde (MDA) content of mice, B is a bar graph of the superoxide dismutase (SOD) content of mice, C is a bar graph of the CAT content of mice, and D is a bar graph of the GSH content of mice, in which # is P <0.01 as compared with the con.group, # is P <0.05 as compared with the mod.group, and P <0.01 as compared with the mod.group.

Fig. 5 is a graph showing the results of the effect of the medicated wine of the present invention on the liver glycogen and muscle glycogen contents of mice (n 8), wherein a is a bar graph of liver glycogen contents of mice, B is a bar graph of muscle glycogen contents of mice, and in the graphs, # # is P <0.01 as compared with con.

In fig. 1-5, con represents blank group, mod represents model group, spi represents positive group, and GRZ1, 2, and 3 represent experimental groups 1, 2, and 3, respectively.

Detailed Description

Example 1

1. Prescription: 50g of mangosteen, 10g of beautiful millettia root, 10g of radix fici simplicissimae, 2g of dried orange peel and 2g of liquorice.

2. The preparation method comprises the following steps:

(1) taking raw material medicines of fructus rhodomyrti, radix millettiae speciosae, radix fici simplicissimae, pericarpium citri reticulatae and liquorice, adding 9 times of 40-degree edible rice wine produced by Guangdong Chenghai wine plant Gmbycis, soaking for 38 days, and filtering to remove residues to obtain a medicinal liquor stock solution;

(2) adding 50g of trehalose into the prepared medicinal liquor stock solution, stirring uniformly, and adding 40-degree edible rice wine produced by Guangdong Chenghai wine plant GmbH to a constant volume of 1000ml to obtain the medicinal liquor.

Example 2

1. Prescription: 54g of mangosteen, 8g of beautiful millettia root, 8g of radix fici simplicissimae, 1.5g of dried orange peel and 2.5g of liquorice.

2. The preparation method comprises the following steps:

(1) taking raw material medicines of fructus rhodomyrti, radix millettiae speciosae, radix fici simplicissimae, pericarpium citri reticulatae and liquorice, adding 12 times of 30-degree edible rice wine produced by Guangdong cis-De wine plant Limited, soaking for 12 days, and filtering to remove residues to obtain a medicinal liquor stock solution;

(2) adding 50g of trehalose into the prepared medicinal liquor stock solution, stirring uniformly, and adding 30-degree edible rice wine produced by Guangdong Shunde wine plant Limited to reach a constant volume of 1000ml to obtain the medicinal liquor.

Example 3

1. Prescription: 46g of mangosteen, 12g of beautiful millettia root, 12g of radix fici simplicissimae, 2.5g of tangerine peel and 1.5g of liquorice.

2. The preparation method comprises the following steps:

(1) taking raw material medicines of fructus rhodomyrti, radix millettiae speciosae, radix fici simplicissimae, pericarpium citri reticulatae and liquorice, adding 8 times of 50-degree edible rice wine produced by Jiujiang wine plant Gmbycis in Guangdong province, soaking for 60 days, and filtering to remove residues to obtain a medicinal liquor stock solution;

(2) adding 50g of trehalose into the prepared medicinal liquor stock solution, stirring uniformly, and adding 50-degree edible rice wine produced by Jiujiang wine works Limited in Guangdong province to a constant volume of 1000ml to obtain the medicinal liquor.

Claims (4)

1. An antifatigue medicated liquor comprises edible rice wine and effective components; the effective components in each liter of the medicinal liquor are prepared from the following raw material medicines in parts by weight: 45-55 g of mangosteen, 8-12 g of beautiful millettia root, 8-12 g of radix fici simplicissimae, 1.5-2.5 g of dried orange peel and 1.5-2.5 g of liquorice.

2. The anti-fatigue medicinal liquor according to claim 1, wherein the effective components in each liter of the medicinal liquor are prepared from the following raw material medicines in parts by weight: 50g of mangosteen, 10g of beautiful millettia root, 10g of radix fici simplicissimae, 2g of dried orange peel and 2g of liquorice.

3. The anti-fatigue medicinal liquor according to claim 1 or 2, which is prepared by the following method:

(1) taking the raw materials, adding 8-10 times of edible rice wine, soaking for 15-60 days, filtering and removing residues to obtain a medicinal liquor stock solution;

(2) adding a proper amount of trehalose into the prepared medicinal liquor stock solution, uniformly stirring, and then adding edible rice wine to a constant volume of 1000ml to obtain the medicinal liquor.

4. The anti-fatigue medicinal liquor according to claim 3, wherein the alcoholic strength of the edible rice wine for soaking in the step (1) of the method is the same as that of the edible rice wine for volume determination in the step (2).

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