CN109223245A - A kind of artificial insemination method of the kind goose sperm suitable for refrigeration - Google Patents
A kind of artificial insemination method of the kind goose sperm suitable for refrigeration Download PDFInfo
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- CN109223245A CN109223245A CN201811122110.XA CN201811122110A CN109223245A CN 109223245 A CN109223245 A CN 109223245A CN 201811122110 A CN201811122110 A CN 201811122110A CN 109223245 A CN109223245 A CN 109223245A
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- 230000000694 effects Effects 0.000 claims abstract description 20
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- 238000012549 training Methods 0.000 claims abstract description 16
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- 241001133760 Acoelorraphe Species 0.000 claims description 9
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- 210000003746 feather Anatomy 0.000 claims description 6
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- 238000011161 development Methods 0.000 claims description 4
- 230000018109 developmental process Effects 0.000 claims description 4
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims description 3
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 claims description 3
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 3
- 230000001143 conditioned effect Effects 0.000 claims description 3
- 210000002969 egg yolk Anatomy 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
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- QEEAPRPFLLJWCF-UHFFFAOYSA-K potassium citrate (anhydrous) Chemical compound [K+].[K+].[K+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O QEEAPRPFLLJWCF-UHFFFAOYSA-K 0.000 claims description 3
- 235000011082 potassium citrates Nutrition 0.000 claims description 3
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- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 claims description 3
- 239000001632 sodium acetate Substances 0.000 claims description 3
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- 238000004519 manufacturing process Methods 0.000 abstract description 7
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- 239000003163 gonadal steroid hormone Substances 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
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- 239000000463 material Substances 0.000 description 1
- 235000021590 normal diet Nutrition 0.000 description 1
- 230000008775 paternal effect Effects 0.000 description 1
- 230000035946 sexual desire Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
- A61D19/021—Apparatus for collecting seminal fluids; Artificial vaginas
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
- A61D19/022—Containers for animal semen, e.g. pouches or vials ; Methods or apparatus for treating or handling animal semen containers, e.g. filling or closing
- A61D19/025—Containers for animal semen, e.g. pouches or vials ; Methods or apparatus for treating or handling animal semen containers, e.g. filling or closing with means for controlling the temperature, e.g. heating or cooling
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
- A61D19/027—Devices for injecting semen into animals, e.g. syringes, guns, probes
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Zoology (AREA)
- Animal Behavior & Ethology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Reproductive Health (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Animal Husbandry (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of artificial insemination methods of kind goose sperm suitable for refrigeration.The kind of eugenic kind of goose is obtained using the method for population comparison first, artificial semenpicking is carried out to common adult gander in this course, then sperm quantity and activity are compared under given conditions, the still very high goose of sperm fertilization rate is as kind of a goose after choosing refrigeration.Then induction type raising is carried out in feed lot.Herbal medicine of the addition rich in elements such as selenium, zinc, iron in feed, and it is aided with vitamin E；It mends and shines in advance, extend light application time, gander is made to enter breeding time in advance；Physical fitness training is carried out, the continuous number of ejaculations per of goose is extended.By above-mentioned method, egg production and laying rate are improved, especially solves the problems, such as that semen collection and semen deposition operation cannot carry out in time.
Description
Technical field
The present invention relates to a kind of artificial insemination methods, cannot carry out the kind of semen deposition operation at once especially after semen collection
Goose field variety selection and the preservation of sperm.
Technical background
It is got more and more attention with the industrialization of the fast development with aquatic bird industry, especially goose, large-scale cultivation.Goose conduct
A kind of aquatic bird has the habit to mate in water, although mating rate of fertilization ratio is high in land in water, uncontrollability is very
Greatly.Mainly there are three modes for existing natural mating, are big population breeding, small breeding and individual Dan Peisan kind respectively；In order to
It obtains paternal family and carries out progeny testing, also have by the way of shift system of mating.
In order to obtain better fertility rate of hatching egg, human assistance breeding, the mode of artificial insemination is gradually used by people.
But the kind of goose is numerous, there is lion-headed goose, white goose, wild goose goose etc., an outstanding kind goose kind how is obtained, for another example and at this
The but cultivation of puzzlement kind goose always of an optimal rate of fertilization is obtained in kind.It cannot be carried out immediately especially after semen collection
The farm of semen deposition operation, and gander semen collection Best Times are the morning, and the best Inseminated time of female goose is afternoon.
Summary of the invention:
The purpose of the present invention is to provide a kind of methods for obtaining outstanding kind of goose kind and optimal semen deposition operation.
The present invention obtains optimal kind goose artificial insemination method, a kind of kind goose that can be used for refrigerating using following method
The artificial insemination method of sperm:
Determine the kind of aristogenesis kind of gander:
A) it chooses medium-sized goose of the adult weight in 5-6.5kg and figure is chosen in each kind goose by observation as kind goose
Stalwartness, sound is loud and clear, and feather color is distinct, and reproductive development is normally grown up gander each 20, demarcated respectively；
B) artificial semenpicking carried out to each kind gander of selection, continuous 7 days, 1 time a day, using massage respectively to calibration at
Year gander progress semen collection；
C) sperm quality is compared, after using dilution mixing, refrigeration 24 hours, cold preservation time knot is carried out at 3 DEG C
Spermatozoon activity and quantity are compared after beam；Selection wherein has four times and its above sperm quantity is not less than 600,000,000/milli
It rises, kind of kind goose of the vigor not less than 0.6 as eugenic kind of gander.
Kind goose is fed:
A) in the kind of selected eugenic kind of gander, choose 40% genital development is normal, gander that posture is excellent as kind
Goose is raised, daily throw in feed regularly, and addition is rich in the herb ingredients of selenium, zinc, ferro element in feed, and is aided with vitamin E；
B) physical training is carried out, water surface swimming for 1 hours is respectively carried out in every morning and afternoon, can make during swimming
Driving for certain frequency is carried out with stock, so that goose is carried out physical fitness training, but this process cannot frighten gander；
C) artificial mend of 30 beginnings is shone before entering breeding time, and on the basis of original illumination, each goose house increases by one in 20 square metres
The incandescent lamp of a 30W, daily light application time extend 4 hours, continue 30 days, stimulate the genital development of gander；
D) 15 days stopping swimming exercises before semen collection prohibit water, carry out land and raise and train, continue physical fitness training, daily with stock
It carries out driving gander and run, gander cannot be arrived to scaring, start to simulate artificial semenpicking after 7 days, establish the conditioned reflex of massage,
The foundation of the condition launch time was completed at 7 days, and therefrom the gander of selection 50% being highly sexed uses as last kind goose.
Artificial semenpicking:
A) temperature is controlled before semen collection, and sufficient sound insulation is accomplished to semen collection room, the music releived can be played, with the mould of the Nature
Onomatopoeia sound is best, after kind of goose is sufficiently stable, starts semen collection；
B) kind of a goose is placed on knee, left hand thumb is separated with remaining four finger, and natural torsion, the centre of the palm is downward, is placed on goose back
It between two wings, is stroked repeatedly to root of the tail portion, starts to have an effect when the centre of the palm is close to root of the tail portion, squeeze the root of the tail portion excitement of goose,
When palm leaves root of the tail portion every time, thumb and remaining four finger clamp tail feathers and firmly clamp pause 0.5 second, simultaneously, right hand thumb
Refer to and remaining four is directed toward its postabdomen to cloaca ring repeated massage, until cloaca Surrounding muscles are congested, left hand and the right side at this time
Hand massages congested cloaca simultaneously, and penis releases, continues to massage, after penis sufficiently erects, utilize semen collector semen collection；
C) the semen collection time is set in daily morning 9:00-10:00, semen collection in 2 days 1 time, has three days off after continuous semen collection 5 times.
Semen routine:
A) sperm obtained needs to be saved using dilution full of nutrition, dilution full of nutrition because activity is vigorous
Liquid provides the energy for sperm, guarantees the balance of spermatid infiltration and ion, in the dilution that the present invention uses are as follows: steams at 90 milliliters
1 part of glucose, 0.2 part of inositol, 1.5 parts of glutamic acid, 1.5 parts of sodium chloride, 0.5 part of sodium acetate, potassium citrate 0.1 are added in distilled water
Part, 1 part of penicillin, 1 part of streptomysin, 1 part of yolk liquid, defatted milk 1, the above unit is gram；
B) the dilution proportion of dilution and sperm is 4:1, and pH value is adjusted to 7.1, by water-bath into refrigeration at 3 DEG C, after refrigeration
Semen deposition operation is carried out in 24 hours.
Semen deposition operation:
Inseminated time is scheduled on daily female goose in afternoon twice between feeding, it is ensured that the health of semen deposition instrument, most with disposable instrument
It is good.
Beneficial effects of the present invention:
Using above-mentioned technical solution, the purpose of the present invention that can preferably realize.
Firstly, the kind of eugenic kind of goose is obtained using the method for population comparison, in this course to common adult
Gander carries out artificial semenpicking, after being mixed by diluted, refrigerated by 24 hours, then carries out to sperm quantity and activity
Compare, the population for selecting sperm quantity most under the premise of spermatozoon activity is excellent after refrigeration is as eugenic kind of goose；In the present invention
The kind goose of selection still can achieve continuous four sperm quantities after without induction type raising refrigeration 24 hours and reach 600,000,000,
And vigor reaches 0.6.
When kind of feed lot cannot carry out semen deposition operation at once, sperm is diluted using nutrient solution, is refrigerated.The present invention
Middle dilution is full of nutrition, guarantees the generation that can inhibit lactic acid while sperm consumption, while enough in sperm quantity
In the case of can reach the dilution ratio of 4:1.
In subsequent breeding process, 40% posture is chosen in eugenic kind of goose kind and well-developed gander is induced
Formula raising.Herbal medicine of the addition rich in elements such as selenium, zinc, iron in feed, and it is aided with vitamin E, improve the generation rate of sperm；It mentions
Preceding benefit is shone, and extends light application time, gander is made to enter breeding time in advance；Physical fitness training is carried out, the continuous number of ejaculations per of goose is extended.Pass through
Above-mentioned method, further the gander in induction raising range chooses 50% gander being highly sexed again, is finally planted
Goose；The sperm quantity of this kind of goose improves 500,000,000/milliliter, for semen collection production essence age in days increase by 10 days, stablize 210 days with
On, by reasonably feeding and physical fitness training, the continuous number of ejaculations per of gander reach 6 times, then rest again, egg production and production
Egg rate is greatly improved.Method of the invention especially solves the problems, such as that semen collection cannot synchronous progress with semen deposition operation.
Specific embodiment
It further illustrates combined with specific embodiments below:
Firstly, determining the kind of aristogenesis kind of gander:
A) it chooses medium-sized goose of the adult weight in 5-6.5kg and figure is chosen in each kind goose by observation as kind goose
Stalwartness, sound is loud and clear, and feather color is distinct, and reproductive development is normally grown up gander each 20, demarcated respectively；
B) artificial semenpicking carried out to each kind gander of selection, continuous 7 days, 1 time a day, using massage respectively to calibration at
Year gander progress semen collection；
C) sperm quality is compared, after using dilution mixing, refrigeration 24 hours, cold preservation time knot is carried out at 3 DEG C
Spermatozoon activity and quantity are compared after beam；Selection wherein has four times and its above sperm quantity is not less than 600,000,000/milli
It rises, kind of kind goose of the activity not less than 6 one-tenth as eugenic kind of gander.
The goose various types of artificial domestication can be currently carried out, but not all goose is suitable as kind of goose and uses, especially
It is that some kind geese are not appropriate for refrigerating in sperm, or its activity substantially reduces after refrigeration, it would be desirable to first in these geese
Eugenic goose kind is sifted out in kind.
We have selected 3 kinds of domestic geese and a kind of introducing goose in the determination of the kind of eugenic kind of goose, and experiment gained is as follows,
Wherein sperm concentration is hundred million/milliliter, activity for line activities in all sperms 30 minutes ratio, up to 10 one-tenth:
Wherein, kind B, C, D has reached 600,000,000/milliliter and 6 Viability standards, but D kind sperm quantity is substantially better than preceding two
Kind, therefore, we select D kind as the kind of eugenic kind of goose.
Kind goose is fed:
A) in the kind of selected eugenic kind of gander, choose 40% genital development is normal, gander that posture is excellent as kind
Goose is raised, daily throw in feed regularly, and addition is rich in the herb ingredients of selenium, zinc, ferro element in feed, and is aided with vitamin E；
B) physical training is carried out, respectively carry out primary water surface swimming for 1 hours in every morning and afternoon, it can during swimming
To use stock to carry out driving for certain frequency, goose is set to carry out physical fitness training, but this process cannot frighten gander；
C) artificial mend of 30 beginnings is shone before entering breeding time, and on the basis of original illumination, each goose house increases by one in 20 square metres
The incandescent lamp of a 30W, daily light application time extend 4 hours, continue 30 days, stimulate the genital development of gander；
D) 15 days stopping swimming exercises before semen collection prohibit water, carry out land and raise and train, continue physical fitness training, daily with stock
It carries out driving gander and run, gander cannot be frightened, start to simulate artificial semenpicking after 7 days, establish the conditioned reflex of massage, it should
The foundation of condition launch time was completed at 7 days, and therefrom the gander of selection 50% being highly sexed uses as last kind goose.
After obtaining eugenic kind of gander, we will further screen in the group, and carry out to the gander screened
Induction type is fed, and using the genital development of long light stimulation gander, increases the constituent of male sex hormone synthesis in feed,
In order to obtain continuous semen collection, physical training is carried out to gander, improves the sexual desire of these ganders as far as possible.
It is the difference of the sperm using gander method for breeding in the present invention and common raising gander below:
| Increase the medicinal material feed rich in elements such as selenium, zinc, iron in feed, and is aided with vitamin E | Each semen collection sperm quantity increases 300,000,000/milliliter, and can stablize in 3,300,000,000/milliliter |
| Normal diet is fed | 2500000000/milliliter |
| Carry out benefit photograph within 50 days in advance | The age in days of laying eggs of goose increases by 10 days, and can stablize at 210 days or more |
| Normal illumination | The age in days of laying eggs of goose is 200-210 |
| Carry out lasting physical fitness training | Then continuous number of ejaculations per has three days off up to 6 times |
| Naturally stocking | It is only capable of maintaining continuous 4 semen collections |
Artificial semenpicking:
A) temperature is controlled before semen collection, and sufficient sound insulation is accomplished to semen collection room, the music releived can be played, with the mould of the Nature
Onomatopoeia sound is best, after kind of goose is sufficiently stable, starts semen collection；
B) kind of a goose is placed on knee, left hand thumb is separated with remaining four finger, and natural torsion, the centre of the palm is downward, is placed on goose back
It between two wings, is stroked repeatedly to root of the tail portion, starts to have an effect when the centre of the palm is close to root of the tail portion, squeeze the root of the tail portion excitement of goose,
When palm leaves root of the tail portion every time, thumb and remaining four finger clamp tail feathers and firmly clamp pause 0.5 second, simultaneously, right hand thumb
Refer to and remaining four is directed toward its postabdomen to cloaca ring repeated massage, until cloaca Surrounding muscles are congested, left hand and the right side at this time
Hand massages congested cloaca simultaneously, and penis releases, continues to massage, after penis sufficiently erects, utilize semen collector semen collection；
C) the semen collection time is set in daily morning 9:00-10:00, semen collection in 2 days 1 time, has three days off after continuous semen collection 5 times.
Artificial semenpicking process will guarantee that goose is stable, and lather can have an impact the quality of sperm, especially excrement meeting
Pollution is generated to sperm.It infects in order to prevent, semen collection person will carry out disinfection, and semen collection equipment will equally perform hygiene guarantee work,
Preferably with disposable instrument.
Semen routine:
A) sperm obtained needs to be saved using dilution full of nutrition, dilution full of nutrition because activity is vigorous
Liquid provides the energy for sperm, guarantees the balance of spermatid infiltration and ion, in the dilution that the present invention uses are as follows: steams at 90 milliliters
1 part of glucose, 0.2 part of inositol, 1.5 parts of glutamic acid, 1.5 parts of sodium chloride, 0.5 part of sodium acetate, potassium citrate 0.1 are added in distilled water
Part, 1 part of penicillin, 1 part of streptomysin, 1 part of yolk liquid, defatted milk 1, the above unit is gram；
B) the dilution proportion of dilution and sperm is 4:1, and pH value is adjusted to 7.1, by water-bath into refrigeration at 3 DEG C, after refrigeration
Semen deposition operation is carried out in 24 hours.
Semen deposition operation:
Inseminated time is scheduled on daily female goose in afternoon twice between feeding, it is ensured that the health of semen deposition instrument, most with disposable instrument
It is good.
The kind of eugenic kind of goose is obtained using the method for population comparison, in this course to common adult gander into
Row artificial semenpicking after being mixed by diluted, was refrigerated by 24 hours, is then compared to sperm quantity and activity,
The population for selecting sperm quantity most under the premise of spermatozoon activity is excellent after refrigeration is as eugenic kind of goose；It is chosen in the present invention
Kind goose still can achieve continuous four sperm quantities after without induction type raising refrigeration 24 hours and reach 600,000,000, and activity
Reach 6 one-tenth.
When kind of feed lot cannot carry out semen deposition operation at once, sperm is diluted using nutrient solution, is refrigerated.The present invention
Middle dilution is full of nutrition, guarantees the generation that can inhibit lactic acid while sperm consumption, while enough in sperm quantity
In the case of can reach the dilution ratio of 4:1.
In subsequent breeding process, 40% posture is chosen in eugenic kind of goose kind and well-developed gander is induced
Formula raising.Herbal medicine of the addition rich in elements such as selenium, zinc, iron in feed, and it is aided with vitamin E, improve the generation rate of sperm；It mentions
Preceding benefit is shone, and extends light application time, gander is made to enter breeding time in advance；Physical fitness training is carried out, the continuous number of ejaculations per of goose is extended.Pass through
Above-mentioned method, further the gander in induction raising range chooses 50% gander being highly sexed again, is finally planted
Goose；The sperm quantity of this kind of goose improves 500,000,000/milliliter, for semen collection production essence age in days increase by 10 days, stablize 210 days with
On, by reasonably feeding and physical fitness training, the continuous number of ejaculations per of gander reach 6 times, then rest again, egg production and production
Egg rate is greatly improved.
For those skilled in the art, without departing from the structure of the invention, several changes can also be made
Shape and improvement, these should also be as being considered as protection scope of the present invention, these all will not influence the effect and patent that the present invention is implemented
Practicability.
Claims (1)
1. a kind of artificial insemination method of the kind goose sperm suitable for refrigeration, it is characterised in that:
1) kind of eugenic kind of gander is determined:
A) it chooses medium-sized goose of the adult weight in 5-6.5kg and figure is chosen in each kind goose by observation as kind goose
Stalwartness, sound is loud and clear, and feather color is distinct, and reproductive development is normally grown up gander each 20, demarcated respectively；
B) artificial semenpicking carried out to each kind gander of selection, continuous 7 days, 1 time a day, using massage respectively to calibration at
Year gander progress semen collection；
C) sperm quality is compared, after mixing using dilution, refrigeration 24 hours is carried out at 3 DEG C, cold preservation time terminates
The comparison of spermatozoon activity and quantity is carried out afterwards；Selection wherein has four times and its above sperm quantity is not less than 600,000,000/milliliter,
Kind of kind goose of the vigor not less than 0.6 as eugenic kind of gander；
2) kind goose is fed:
A) in the kind of selected eugenic kind of gander, choose 40% genital development is normal, gander that posture is excellent as kind
Goose is raised, daily throw in feed regularly, herb ingredients of the addition rich in elements such as selenium, zinc, iron in feed, and is aided with vitamin
E；
B) physical training is carried out, primary water surface swimming for 1 hours is respectively carried out in every morning and afternoon, makes during swimming
It was once driven with stock every 5 minutes, allows gander to carry out physical fitness training, but this process cannot frighten gander；
C) artificial mend of 30 beginnings is shone before entering breeding time, and on the basis of original illumination, each goose house increases by one in 20 square metres
The incandescent lamp of a 30W, daily light application time extend 4 hours, continue 30 days, stimulate the genital development of gander；
D) 15 days stopping swimming exercises before semen collection prohibit water, carry out land and raise and train, continue physical fitness training, daily with stock
It carries out driving gander and run, gander cannot be frightened, start to simulate artificial semenpicking after 7 days, establish the conditioned reflex of massage, it should
The foundation of condition launch time was completed at 7 days, and therefrom the gander of selection 50% being highly sexed uses as last kind goose；
3) artificial semenpicking:
A) temperature is controlled before semen collection, and sufficient sound insulation is accomplished to semen collection room, the music releived can be played, with the mould of the Nature
Onomatopoeia sound is best, after kind of goose is sufficiently stable, starts semen collection；
B) kind of a goose is placed on knee, left hand thumb is separated with remaining four finger, and natural torsion, the centre of the palm is downward, is placed on goose back
It between two wings, is stroked repeatedly to root of the tail portion, starts to have an effect when the centre of the palm is close to root of the tail portion, squeeze the root of the tail portion excitement of goose,
When palm leaves root of the tail portion every time, thumb and remaining four finger clamp tail feathers and firmly clamp pause 0.5 second, simultaneously, right hand thumb
Refer to and remaining four is directed toward its postabdomen to cloaca ring repeated massage, until cloaca Surrounding muscles are congested, left hand and the right side at this time
Hand massages congested cloaca simultaneously, and penis releases, continues to massage, after penis sufficiently erects, utilize semen collector semen collection；
C) the semen collection time is set in the 9:00-10:00 in the morning, semen collection in 2 days 1 time, has three days off after continuous semen collection 5 times；
4) Semen routine:
A) sperm obtained is saved because activity is vigorous using dilution full of nutrition, is added in 90 milliliters of distilled water
Enter 1 part of glucose, 0.2 part of inositol, 1.5 parts of glutamic acid, 1.5 parts of sodium chloride, 0.5 part of sodium acetate, 0.1 part of potassium citrate, mould
Plain 1 part, 1 part of streptomysin, 1 part of yolk liquid, 1 part of defatted milk be fabricated to dilution, the above unit is gram；
B) the dilution proportion of dilution and sperm is 4:1, and pH value is adjusted to 7.1, by water-bath into refrigeration at 3 DEG C, after refrigeration
Semen deposition operation is carried out in 24 hours；
4) semen deposition operation:
Inseminated time is scheduled on female goose in afternoon twice between feeding, it is ensured that the health of semen deposition instrument, it is best with disposable instrument.
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| CN201811122110.XA CN109223245B (en) | 2018-09-26 | 2018-09-26 | Artificial insemination method of breeding goose sperms suitable for refrigeration |
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