CN1172634A - Silver fox and arctic fox cross-breeding method and semen deposition needle - Google Patents
Silver fox and arctic fox cross-breeding method and semen deposition needle Download PDFInfo
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- CN1172634A CN1172634A CN 96115906 CN96115906A CN1172634A CN 1172634 A CN1172634 A CN 1172634A CN 96115906 CN96115906 CN 96115906 CN 96115906 A CN96115906 A CN 96115906A CN 1172634 A CN1172634 A CN 1172634A
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- fox
- vulpes
- ereota
- cephalanthera
- blume
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Abstract
A hybridization method for silver fox and arctic fox includes such steps as collecting the sperm of male silver fox by artificial massage during its oestrum, constant-temp treatment, addition of diluting liquid as protector, freezing, refrigerating and infusing the thawed sperm of silver fox into the uterus of femal arctic fox during its oestrum by special sperm-infusing needle, so solving the asynchronous problem of their oestrums. The hybridized silver-arctic fox features high disease resistance, high quality of fur, and high farrowing and survival rates.
Description
The invention belongs to artificial insemination method and the device of animal, specifically is a kind of Cephalanthera ereota (Thunb.) Blume. Vulpes hybridization production method and insemination needle.
Now, to the artificial insemination of animal, just be tending towards ripe as the technology of cattle, sheep etc., and Vulpes is a wild animal, its semen collection, cold preservation, insemination technique and cattle, sheep differ widely, and particularly silver fox and blue fox are oestrused asynchronous, choosing spouse property of blue fox is strong, they are difficult to reach the natural mating reproduction, and the genitals of female blue fox is comparatively special, so also will adopt special-purpose spermatic device, Chinese patent database by retrieval, to the artificial insemination of silver fox and blue fox without literary records.
The object of the present invention is to provide a kind of artificial insemination that utilizes, make oestrus nonsynchronous silver fox and blue fox hybridization, cultivate strong, the easy artificial breeding of premunition, fur pin hair is short, Cephalanthera ereota (Thunb.) Blume. Vulpes hybridization production method and the insemination needle of the Cephalanthera ereota (Thunb.) Blume. Vulpes that under hair is flat.
The present invention is achieved by the following scheme:
It is Cephalanthera ereota (Thunb.) Blume. Vulpes hybridization production method; comprise semen collection, cold preservation; semen deposition three parts; gather the seminal fluid of male silver fox in the mode that silver fox is massaged oestrus by hand; handle the back through constant temperature and add the diluent protective agent-80 ℃～closely freezing, cold preservation under-120 ℃ of temperature, the silver fox seminal fluid that will thaw during to oestrus when blue fox is input in the body of female blue fox.
The collection of above-mentioned silver fox seminal fluid is to select strong shape, fur light, male silver fox that testicualr development is good, put it in the fender bracket, flick earlier under the testis number, massage by the three fast one slow rhythm and pace of moving things at its penis sponge body region then, rapidly penis is pulled under the hip when treating corpus cavernosum, the stripping foreskin stimulates glans penis with finger to spongy body, male silver fox is promptly penetrated seminal fluid after several minutes; The cold preservation of seminal fluid is that the seminal fluid that collects is added the diluent protective agent, adopt falling temperature method gradually, when spending 1～1.5 hour temperature dropping to 4～5 ℃, in with temperature refrigerator, preserved 4 hours again, under-80 ℃～-120 ℃ temperature, be refrigerated to graininess then, the graininess seminal fluid is packed in the gauze bag, put into liquid nitrogen container and preserve; The diluent that adds is made up of glycine, sodium citrate, glycerol and distilled water, its proportioning is: glycine: 1.34～1.39 gram glycerol: 3.5～6ml sodium citrate: 0.72～0.78 gram distilled water: 73～78ml, and with glycine: 1.37 gram glycerol: 5mL sodium citrate: 0.75 gram distilled water: 75mL is best proportioning; The female blue fox that to oestrus during semen deposition is put into fender bracket, earlier the vagina conduit is inserted the blue fox vagina, fixes its cervix uteri with left hand under abdomen, the reuse right hand is held insemination needle and is inserted into cervix uteri by the vagina conduit, after exploring cervical orifice gently, put in intrauterine, be input into the good seminal fluid that thaws; The hybridization of Cephalanthera ereota (Thunb.) Blume. Vulpes comprises needle body with insemination needle, the needle body point diameter is thin than the rear end diameter, the needle body leading section is spherical in shape, the long 5.5～7.5cm of its front end, diameter 0.5～1.5cm, long 12～the 14.5cm in rear end, diameter is 1～2.5cm, and needle body the best is the long 6.5cm of front end, diameter 1cm, the long 13cm in rear end, diameter are 2cm.
Describe in further detail below in conjunction with embodiment:
Fig. 1: insemination needle structural representation.
Among the figure: 1 bulb, 2 syringe needles, 3 needle bodies, 4 conjuncted
Select healthy and strong; the fur light; the good male silver fox of testicualr development is in silver fox oestrus; i.e. two Marches; be placed in the fender bracket; slight bullet testis number down; testis is loosened; massage by the three fast one slow rhythm and pace of moving things at penis; then cavernous body of penis expands rapidly; be in the ejaculation state; at this moment immediately penis is pulled under the hip; the strip off foreskin; flick to stimulate glans penis with finger; male silver fox is penetrated seminal fluid in the time of slightly; because of blue fox than silver fox evening in oestrus; so palpus elder generation is with the seminal fluid cold preservation of male silver fox; in order to blue fox during oestrus semen deposition use; the seminal fluid that collects is added the diluent protective agent; when spending 1～5 hour approximately and being cooled to 4～5 ℃ naturally; in with temperature refrigerator, preserved 4 hours; so that detect the sperm survival rate; under-80 ℃～-120 ℃ temperature, be refrigerated to graininess then; after the vigor of checking sperm once more and density, be encased in the gauze bag, put into liquid nitrogen container and preserve, when three～April blue fox during to oestrus; the female blue fox of oestrusing is put in the fender bracket; the vagina conduit is inserted the vixen vagina, fix its cervix uteri with left hand under abdomen, the right hand is held insemination needle and is passed through the vagina conduit to cervix uteri; this moment, bulb was near cervical orifice; syringe needle 2 and needle body 3 are held needle body and are explored cervical orifice with bulb 1 gently in the vagina conduit, and bulb 1 and syringe needle 2 are put in intrauterine; then by conjuncted 4 and needle body 3 the good silver fox seminal fluid that thaws is input to the blue fox intrauterine, the vixen after the semen deposition gets final product by the pregnancy period feeding and management method.
Embodiment 1: just freeze temperature-85 ℃, diluent proportioning:
Glycine: 1.34 grams, sodium citrate: 0.74 gram, glycerol: 4.2ml, distilled water: 74ml, the secondary of inseminating continuously.
Embodiment 2: just freeze temperature-90 ℃, diluent proportioning:
Glycine: 1.35 grams, sodium citrate: 0.75 gram, glycerol: 4.75ml, distilled water: 76ml, the secondary of inseminating continuously.
Embodiment 3: just freeze temperature-120 ℃, diluent proportioning:
Glycine: 1.37 grams, sodium citrate: 0.75 gram, glycerol: 5ml, distilled water: 75ml, inseminate three times continuously.
Embodiment 4: just freeze temperature-118 ℃, diluent proportioning:
Glycine: 1.38 grams, sodium citrate: 0.765 gram, glycerol: 5.5ml, distilled water: 75.5ml, inseminate four times continuously.
Embodiment 5: just freeze temperature-115 ℃, diluent proportioning:
Glycine: 1.39 grams, sodium citrate: 0.78 gram, glycerol: 6ml, distilled water: 78ml, inseminate four times continuously.
Preserve male silver fox seminal fluid with said method, step, give female blue fox artificial insemination, the silver-colored blue fox of hybridization is had the following advantages:
1, premunition is strong, is easy to artificial feeding.
2, fur light, pin hair weak point, under hair are put down.
3, young beastly survival rate height, the incidence of disease is low, and farrowing is many.
Silver-colored blue fox after silver fox and the hybridization of blue fox and the contrast of silver fox and blue fox see the following form:
| Moral character kind energy | The blue fox of the blue fox silver of silver fox |
| The group is long (cm) lint characteristics of long (cm) skin of the young beastly survival rate of litter size (only) animal fodder ratio (%) (%) incidence of disease (%) bodily form all | 4-6 10-12 10-12 5 60 45 98 80 95 2 10 2 75-85 65-70 75-85 85-95 80-85 85-95 pin staple lengths, loose suede is short, close thick pin hair is short, concordant line hypotrichosis black and white suede is thick moderate, grey |
Claims (10)
1, a kind of Cephalanthera ereota (Thunb.) Blume. Vulpes hybridization production method; comprise semen collection, cold preservation, semen deposition three parts; it is characterized in that gathering the seminal fluid of male silver fox in the mode that silver fox is massaged oestrus by hand; handle freezing, the cold preservation under-80 ℃～-120 ℃ temperature of back adding diluent protective agent through constant temperature, the silver fox seminal fluid that will thaw during to oestrus when blue fox is input in the body of female blue fox.
2, Cephalanthera ereota (Thunb.) Blume. Vulpes hybridization production method according to claim 1; the collection that it is characterized in that the silver fox seminal fluid is to select the male silver fox healthy and strong, that fur is bright, testicualr development is good; put it in the fender bracket; flick earlier under the testis number; at its penis sponge body region rhythm and pace of moving things massage is arranged then, rapidly penis is pulled under the hip when treating corpus cavernosum, the stripping foreskin is to spongy body; stimulate glans penis with finger, male silver fox is promptly penetrated seminal fluid after several minutes.
3, Cephalanthera ereota (Thunb.) Blume. Vulpes hybridization production method according to claim 2, it is characterized in that can be by the three fast one slow rhythm and pace of moving things to the massage of cavernous body of penis.
4, Cephalanthera ereota (Thunb.) Blume. Vulpes hybridization production method according to claim 1; the cold preservation that it is characterized in that seminal fluid is that the seminal fluid that collects is added the diluent protective agent; adopt falling temperature method gradually; when spending 1～1.5 hour temperature dropping to 4～5 ℃; in with temperature refrigerator, preserved 4 hours again; under-80 ℃～120 ℃ temperature, be refrigerated to graininess then, the graininess seminal fluid is packed in the gauze bag, put into liquid nitrogen container and preserve.
5, according to claim 1,3 described Cephalanthera ereota (Thunb.) Blume. Vulpes hybridization production methods, it is characterized in that diluent is made up of glycine, sodium citrate, glycerol and distilled water, its proportioning is:
Glycine: 1.34～1.39 gram glycerol: 3.5～6ml
Sodium citrate: 0.72～0.78 gram distilled water: 73～78ml
Its best proportioning is:
Glycine: 1.37 gram glycerol: 5mL
Sodium citrate: 0.75 gram distilled water: 75mL
6, Cephalanthera ereota (Thunb.) Blume. Vulpes hybridization production method according to claim 1; it is characterized in that semen deposition is that female female blue fox of oestrusing is put into fender bracket; earlier the vagina conduit is inserted the blue fox vagina; under abdomen, fix its cervix uteri with left hand; the reuse right hand is held insemination needle and is inserted into cervix uteri by the vagina conduit; after exploring cervical orifice gently, put in intrauterine, be input into the good seminal fluid that thaws.
7, according to claim 1,6 described Cephalanthera ereota (Thunb.) Blume. Vulpes hybridization production methods, it is characterized in that semen deposition in 2-5 days continuously, semen deposition every day once, the smart back vixen of being totally lost is by the pregnancy period feeding and management.
8, insemination needle is used in a kind of Cephalanthera ereota (Thunb.) Blume. Vulpes hybridization, comprises needle body, it is characterized in that the needle body point diameter is thin than the rear end diameter, and the needle body leading section is spherical in shape.
9, insemination needle is used in Cephalanthera ereota (Thunb.) Blume. Vulpes hybridization according to claim 8, it is characterized in that the long 5.5～7.5cm of needle body front end, diameter 0.5～1.5cm, and the long 12～14.5cm in rear end, diameter is 1～2.5cm.
10, insemination needle is used in Cephalanthera ereota (Thunb.) Blume. Vulpes hybridization according to claim 8, it is characterized in that needle body the best is the long 6.5cm of front end, diameter 1cm, and the long 13cm in rear end, diameter are 2cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 96115906 CN1172634A (en) | 1996-08-01 | 1996-08-01 | Silver fox and arctic fox cross-breeding method and semen deposition needle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 96115906 CN1172634A (en) | 1996-08-01 | 1996-08-01 | Silver fox and arctic fox cross-breeding method and semen deposition needle |
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| Publication Number | Publication Date |
|---|---|
| CN1172634A true CN1172634A (en) | 1998-02-11 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 96115906 Pending CN1172634A (en) | 1996-08-01 | 1996-08-01 | Silver fox and arctic fox cross-breeding method and semen deposition needle |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105078611A (en) * | 2015-09-21 | 2015-11-25 | 广西参皇养殖集团有限公司 | Poultry insemination apparatus |
| CN109223245A (en) * | 2018-09-26 | 2019-01-18 | 江苏农牧科技职业学院 | A kind of artificial insemination method of the kind goose sperm suitable for refrigeration |
| CN109843217A (en) * | 2016-10-18 | 2019-06-04 | 伺马般有限公司 | Device for mammalian artificial's insemination |
-
1996
- 1996-08-01 CN CN 96115906 patent/CN1172634A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105078611A (en) * | 2015-09-21 | 2015-11-25 | 广西参皇养殖集团有限公司 | Poultry insemination apparatus |
| CN109843217A (en) * | 2016-10-18 | 2019-06-04 | 伺马般有限公司 | Device for mammalian artificial's insemination |
| CN109843217B (en) * | 2016-10-18 | 2021-03-12 | 伺马般有限公司 | Device for artificial insemination of mammals |
| US11529222B2 (en) | 2016-10-18 | 2022-12-20 | Smartbow Gmbh | Device for artificially inseminating a mammal |
| CN109223245A (en) * | 2018-09-26 | 2019-01-18 | 江苏农牧科技职业学院 | A kind of artificial insemination method of the kind goose sperm suitable for refrigeration |
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