CN109090025A - A kind of crow bone sheep rapid propagation method - Google Patents
A kind of crow bone sheep rapid propagation method Download PDFInfo
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- CN109090025A CN109090025A CN201810845158.7A CN201810845158A CN109090025A CN 109090025 A CN109090025 A CN 109090025A CN 201810845158 A CN201810845158 A CN 201810845158A CN 109090025 A CN109090025 A CN 109090025A
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- 102000002322 Egg Proteins Human genes 0.000 claims description 4
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- VJGGHXVGBSZVMZ-QIZQQNKQSA-N Cloprostenol Chemical compound C([C@H](O)\C=C\[C@@H]1[C@H]([C@@H](O)C[C@H]1O)C\C=C/CCCC(O)=O)OC1=CC=CC(Cl)=C1 VJGGHXVGBSZVMZ-QIZQQNKQSA-N 0.000 claims description 3
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- 239000003708 ampul Substances 0.000 claims description 3
- 229960004407 chorionic gonadotrophin Drugs 0.000 claims description 3
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- 210000004246 corpus luteum Anatomy 0.000 claims description 3
- 239000003085 diluting agent Substances 0.000 claims description 3
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- 229910052731 fluorine Inorganic materials 0.000 claims description 3
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- 238000010254 subcutaneous injection Methods 0.000 claims description 3
- 239000007929 subcutaneous injection Substances 0.000 claims description 3
- 210000001550 testis Anatomy 0.000 claims description 3
- 210000001177 vas deferen Anatomy 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 210000004907 gland Anatomy 0.000 claims description 2
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- 235000013601 eggs Nutrition 0.000 claims 1
- 239000006210 lotion Substances 0.000 claims 1
- 230000019100 sperm motility Effects 0.000 claims 1
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- 238000005516 engineering process Methods 0.000 abstract description 10
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- 210000000582 semen Anatomy 0.000 abstract 2
- 108010031480 Artificial Receptors Proteins 0.000 abstract 1
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 4
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- 241000287828 Gallus gallus Species 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
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- 238000001727 in vivo Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 210000003460 periosteum Anatomy 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Prostheses (AREA)
Abstract
The invention discloses a kind of black bone sheep rapid propagation methods, the following steps are included: choosing outstanding black bone sheep ram, polyembryony crow bone sheep ewe is as donor and sheep ewe as receptor, artificial semenpicking is carried out to the black bone sheep ram of selection, to the semen dilution and freezen protective of acquisition, 3-4 days before arriving to the black bone sheep ewe heat of selection, carry out superfecundation, artificial insemination is carried out to the black bone sheep ewe after superfecundation, embryo method is adopted by operation to the black bone sheep ewe after artificial insemination and carries out embryo collection, Estrus synchronization is carried out to the polyembryony sheep ewe of selection, embryo transfer is carried out to the sheep ewe after estrus synchronization, to the method for semen dilution and freezen protective, the method of superfecundation, the method of estrus synchronization and the method for embryo transfer carry out technical parameter optimization.A kind of black bone sheep rapid propagation method of the invention carries out technical optimization to the rapid propagation method of black bone sheep with new technology and a large amount of experimental data, establishes the distinctive fast numerous novel matched technology of black bone sheep.
Description
Technical field
The present invention relates to black bone sheep reproduction technique field more particularly to a kind of black bone sheep rapid propagation methods.
Background technique
Black bone sheep originates in the Yunnan Province town Lan Pingxian Tong Dian crossbow village, at present black its source of bone sheep of country each province and city cultivation
It is all to come from the village.It is the peerless local rareness species resource in the world.Black bone sheep is determined as by China scientist in addition to crow
Second of black-boned characteristics animal with hereditability energy other than bone chicken.Black-boned characteristics most directly performance be exactly black periosteum with
Black meat.From 2001 Lisu Autonomous Prefecture of Nujiang of Yunnan Province Bai-Pumi Autonomous County of Lanping find for the first time black bone sheep with
Come, measure by analysis, the black matter of black bone sheep is as caused by the melanin of high-content, and this black-boned characteristics tool is heritable
Property, it is very rare genetic resources.Black bone sheep be so far the mankind have found unique contain a large amount of melanin in vivo
Mammal, have high medicinal and health value.
Black bone sheep is the extraordinary sheep variety resource of discovery, and artificial insemination, superfecundation, the hair phase same period, embryo transfer are
Excellent drove is fast rapid-result to expand numerous main breeding new technology, but its breeding physiology feature of the sheep of different cultivars is there are difference, because
This needs to optimize associated technical parameters by experiment, establishes the distinctive fast numerous novel matched technology of black bone sheep.
Summary of the invention
The object of the present invention is to provide a kind of distinctive quick breeding technology methods of black bone sheep.
In order to solve the above technical problems, the technical scheme is that
A kind of crow bone sheep rapid propagation method, comprising the following steps:
(1) age is chosen at 2-5 years old, and constitution is strong, and reproductive development is complete, full of vitality, sperm when in use
For vigor 0.7 or more, normal ejaculation amount is 0.8ml-1.2ml, and the preferable outstanding black bone sheep ram of density chooses the age in 2-5
In year, constitution is strong, and polyembryony crow bone sheep ewe chooses the age at 2-5 years old as donor, and figure is big, and the strong polyembryony sheep of reproductive capacity is female
Sheep is as receptor；
(2) feeding management is reinforced to the black bone sheep ram that step (1) is chosen, carries out artificial semenpicking, crow is massaged in daily timing
Bone sheep ram testis 10-15 minutes, the next day every 12 milliliters of third cortisol of injection, daily artificial semenpicking 1-2 times, to the essence of acquisition
Liquid dilution and freezen protective are diluted formula of liquid first and prepare, and 3 grams of glucose, 3 grams of sodium citrate add distilled water to 100
Milliliter after dissolution, water-bath scalding 20 minutes, adds 100,000 unit of penicillin, 0.1 gram of streptomysin, takes 80 milliliters to add after cooling
20 milliliters of yolk, A liquid is made, takes 44 milliliters of A liquid, then 6 milliliters of glycerol adding obtained B liquid check motility rate in 0.6 or more person
Freezing carries out 1: 1.5 times of dilution with A liquid (30 DEG C) at 30 DEG C, wraps 8 layers of gauze and is placed on pre-cooling cooling 2~3 in 4 DEG C of refrigerators
Hour, the glycerinated B liquid at 4 DEG C plus with A liquid equivalent shakes up, and final dilution ratio is 1: 3 times of dilution, immediately in fluorine plate
Upper drop is frozen into 0.1 milliliter of particle, takes 94 milliliters of this diluent A liquid, 6 milliliters of glycerol adding, synthesizes a liquid, so that it may srozen tube
Sperm；
(3) 3-4 days before arriving to the black bone sheep ewe heat that step (1) is chosen, muscle or subcutaneous injection pregnant mare serum promote
Gonadal hormone 600-1100 international unit is bred after there is heat, and in same day muscle or intravenous injection human chorionic gonadotrophin
Hormone 500-750 international unit, can superfecundation；
(4) artificial insemination is carried out to the black bone sheep ewe after step (3) superfecundation, cleans black bone sheep ewe vaginal orifice, uses
Prolapsus appliance insertion intravaginal is simultaneously rotated by 90 °, and opens prolapsus appliance, and vas deferens is inserted into cervical orifice 1 centimetre, is then slightly moved back a little
Sperm can be pushed into ostium of uterus neck, the sperm 1ml after input dilution, can again defeated second after inputting 8 hours for the first time, with
Just guarantee quasi- tire rate；
(5) embryo method is adopted by operation to the black bone sheep ewe after step (4) artificial insemination and carries out embryo collection；
(6) the polyembryony sheep ewe that step (1) is chosen is carried out at the estrus synchronization that progestational hormone is combined with prostaglandin
Progestational hormone vaginal plug, in any one day in sheep ewe oestrous cycle, is placed on the vagina deep of sheep processed, taken within 14 days by reason
Out, bolt intramuscular injection pregnant mare serum gonadotrop(h)in (PMSG) simultaneously being taken, sheep concentrates heat after 1-3 days, after sheep ewe heat, number
Day to intrauterine infusion intramuscular injection prostaglandin living or Cloprostenol, heat height synchronization can be made, inject 1 time, about
Make the ewe estrus synchronization of 60%-70%, the 2nd injection is separated by 8-9 days, is handled 7-9 days, is being ended processing with progestin preparation
First 1 day, 1 prostaglandin of intramuscular injection；
(7) by step (5) collect embryo to after step (6) estrus synchronization sheep ewe carry out embryo transfer, by
Smart ovum and mulberry body are transplanted to fallopian tubal, do a kerf in sheep ewe abdomen, find the ovary of ovulation side and observe corpus luteum
Developmental state, embryo, which is injected into ipsilateral cornua uteri upper end or ampulla of uterine tube, with syringe or grafts need to only take on a small quantity
Flushing liquor connection embryo inject together.
After above-mentioned technical proposal, the beneficial effects of the present invention are:
The way of superfecundation processing is to inject promoting sexual gland hormone to donor, makes delivery ratio nature situation of a dam
Under mostly several times to more than ten times of ovum, for breeding or artificial insemination and body early embryo culture.
Embryo transfer, also known as zygote transplation refer to the body early embryo of jenny, or by vitro fertilization and its
The embryo that his mode obtains transplants in jenny body, is allowed to continue to the technology that development is new individual.
Estrus synchronization refers to the process that a group dam oestrous cycle is artificially controlled and adjusted using certain hormone preparations, makes
Concentrate heat in the given time.
A kind of black bone sheep rapid propagation method of the invention, it is with new technology and largely real by the step of science
It tests data and technical optimization is carried out to the rapid propagation method of black bone sheep, improve black bone sheep ewe individual reproduction speed and utilization
Efficiency.The distinctive fast numerous novel matched technology of black bone sheep is established, provides technology guarantor for the development and utilization of black bone sheep variety resource
Barrier.
Detailed description of the invention
The following drawings are only intended to schematically illustrate and explain the present invention, not delimit the scope of the invention.Wherein:
Fig. 1 is a kind of black bone sheep rapid propagation method flow chart of steps of the invention.
Specific embodiment
Specific embodiments of the present invention are described below, illustrated embodiment is served only for explaining the present invention, is not intended to
It limits the scope of the invention.
Referring to Fig.1, a kind of black bone sheep rapid propagation method of the present embodiment, comprising the following steps:
(1) age is chosen at 2-5 years old, and constitution is strong, and reproductive development is complete, full of vitality, sperm when in use
For vigor 0.7 or more, normal ejaculation amount is 0.8ml-1.2ml, and the preferable outstanding black bone sheep ram of density chooses the age in 2-5
In year, constitution is strong, and polyembryony crow bone sheep ewe chooses the age at 2-5 years old as donor, and figure is big, and the strong polyembryony sheep of reproductive capacity is female
Sheep is as receptor；
(2) feeding management is reinforced to the black bone sheep ram that step (1) is chosen, carries out artificial semenpicking, crow is massaged in daily timing
Bone sheep ram testis 10-15 minutes, the next day every 12 milliliters of third cortisol of injection, daily artificial semenpicking 1-2 times, to the essence of acquisition
Liquid dilution and freezen protective are diluted formula of liquid first and prepare, and 3 grams of glucose, 3 grams of sodium citrate add distilled water to 100
Milliliter after dissolution, water-bath scalding 20 minutes, adds 100,000 unit of penicillin, 0.1 gram of streptomysin, takes 80 milliliters to add after cooling
20 milliliters of yolk, A liquid is made, takes 44 milliliters of A liquid, then 6 milliliters of glycerol adding obtained B liquid check motility rate in 0.6 or more person
Freezing carries out 1: 1.5 times of dilution with A liquid (30 DEG C) at 30 DEG C, wraps 8 layers of gauze and is placed on pre-cooling cooling 2~3 in 4 DEG C of refrigerators
Hour, the glycerinated B liquid at 4 DEG C plus with A liquid equivalent shakes up, and final dilution ratio is 1: 3 times of dilution, immediately in fluorine plate
Upper drop is frozen into 0.1 milliliter of particle, takes 94 milliliters of this diluent A liquid, 6 milliliters of glycerol adding, synthesizes a liquid, so that it may srozen tube
Sperm；
(3) 3-4 days before arriving to the black bone sheep ewe heat that step (1) is chosen, muscle or subcutaneous injection pregnant mare serum promote
Gonadal hormone 600-1100 international unit is bred after there is heat, and in same day muscle or intravenous injection human chorionic gonadotrophin
Hormone 500-750 international unit, can superfecundation；
(4) artificial insemination is carried out to the black bone sheep ewe after step (3) superfecundation, cleans black bone sheep ewe vaginal orifice, uses
Prolapsus appliance insertion intravaginal is simultaneously rotated by 90 °, and opens prolapsus appliance, and vas deferens is inserted into cervical orifice 1 centimetre, is then slightly moved back a little
Sperm can be pushed into ostium of uterus neck, the sperm 1ml after input dilution, can again defeated second after inputting 8 hours for the first time, with
Just guarantee quasi- tire rate；
(5) embryo method is adopted by operation to the black bone sheep ewe after step (4) artificial insemination and carries out embryo collection；
(6) the polyembryony sheep ewe that step (1) is chosen is carried out at the estrus synchronization that progestational hormone is combined with prostaglandin
Progestational hormone vaginal plug, in any one day in sheep ewe oestrous cycle, is placed on the vagina deep of sheep processed, taken within 14 days by reason
Out, bolt intramuscular injection pregnant mare serum gonadotrop(h)in (PMSG) simultaneously being taken, sheep concentrates heat after 1-3 days, after sheep ewe heat, number
Day to intrauterine infusion intramuscular injection prostaglandin living or Cloprostenol, heat height synchronization can be made, inject 1 time, about
Make the ewe estrus synchronization of 60%-70%, the 2nd injection is separated by 8-9 days, is handled 7-9 days, is being ended processing with progestin preparation
First 1 day, 1 prostaglandin of intramuscular injection；
(7) by step (5) collect embryo to after step (6) estrus synchronization sheep ewe carry out embryo transfer, by
Smart ovum and mulberry body are transplanted to fallopian tubal, do a kerf in sheep ewe abdomen, find the ovary of ovulation side and observe corpus luteum
Developmental state, embryo, which is injected into ipsilateral cornua uteri upper end or ampulla of uterine tube, with syringe or grafts need to only take on a small quantity
Flushing liquor connection embryo inject together.
Black bone sheep ram 2 is selected, black bone sheep ewe 30, sheep ewe 30, crow bone sheep according to the invention is quick
Propagation method, experimental data are as follows:
A. black bone sheep artificial insemination pregnancy rate >=65%；
B. embryo number >=6.5 piece can be used by surpassing row；
C. embryo transplantation pregnancy rate >=75%.
Indices are superior, effectively increase black bone sheep ewe individual reproduction speed and utilization efficiency.
The above is the citing of best mode for carrying out the invention, wherein the part that do not address in detail is that this field is common
The common knowledge of technical staff.Protection scope of the present invention is based on the contents of the claims, any based on technology of the invention
The equivalent transformation of enlightenment and progress, also within protection scope of the present invention.
Claims (1)
1. a kind of crow bone sheep rapid propagation method, which comprises the following steps:
(1) age is chosen at 2-5 years old, and constitution is strong, and reproductive development is complete, full of vitality, sperm motility when in use
0.7 or more, normal ejaculation amount is 0.8ml-1.2ml, the preferable outstanding black bone sheep ram of density, chooses the age at 2-5 years old, body
Matter is strong, and polyembryony crow bone sheep ewe chooses the age at 2-5 years old, figure is big, the strong polyembryony sheep ewe conduct of reproductive capacity as donor
Receptor；
(2) feeding management is reinforced to the black bone sheep ram that step (1) is chosen, carries out artificial semenpicking, black bone sheep is massaged in daily timing
Ram testis 10-15 minutes, the next day every 12 milliliters of third cortisol of injection, daily artificial semenpicking 1-2 times is dilute to the sperm of acquisition
It releases and freezen protective, be diluted formula of liquid first and prepare, 3 grams of glucose, 3 grams of sodium citrate, add distilled water to 100 millis
It rises, after dissolution, water-bath scalding 20 minutes, 100,000 unit of penicillin is added after cooling, 0.1 gram of streptomysin, takes 80 milliliters plus ovum
It is 20 milliliters yellow, A liquid is made, takes 44 milliliters of A liquid, then 6 milliliters of glycerol adding obtained B liquid check that motility rate can be cold in 0.6 or more person
Freeze, carry out 1: 1.5 times of dilution with A liquid (30 DEG C) at 30 DEG C, wrap 8 layers of gauze be placed in 4 DEG C of refrigerators be pre-chilled cool down it is 2~3 small
When, the glycerinated B liquid at 4 DEG C plus with A liquid equivalent shakes up, and final dilution ratio is 1: 3 times of dilution, immediately on fluorine plate
Drop is frozen into 0.1 milliliter of particle, takes 94 milliliters of this diluent A liquid, 6 milliliters of glycerol adding, synthesizes a liquid, so that it may srozen tube essence
Liquid；
(3) 3-4 days before arriving to the black bone sheep ewe heat that step (1) is chosen, muscle or subcutaneous injection pregnant mare serum promote sexual gland
Hormone 600-1100 international unit is bred after there is heat, and in same day muscle or intravenous injection human chorionic gonadotrophin
500-750 international unit, can superfecundation；
(4) artificial insemination is carried out to the black bone sheep ewe after step (3) superfecundation, cleans black bone sheep ewe vaginal orifice, with opening vagina
Device insertion intravaginal is simultaneously rotated by 90 °, and opens prolapsus appliance, and vas deferens is inserted into cervical orifice 1 centimetre, is then slightly moved back a little
Sperm is pushed into ostium of uterus neck, the sperm 1ml after input dilution, can again defeated second after inputting 8 hours for the first time, to protect
Demonstrate,prove quasi- tire rate；
(5) embryo method is adopted by operation to the black bone sheep ewe after step (4) artificial insemination and carries out embryo collection；
(6) Estrus synchronization that progestational hormone is combined with prostaglandin is carried out to the polyembryony sheep ewe that step (1) is chosen,
Progestational hormone vaginal plug is placed on the vagina deep of sheep processed by any one day in sheep ewe oestrous cycle, is taken out within 14 days,
Take bolt simultaneously intramuscular injection pregnant mare serum gonadotrop(h)in (PMSG), after 1-3 days sheep concentration heat, after sheep ewe heat, a few days to
Intrauterine infusion intramuscular injection prostaglandin living or Cloprostenol, can make heat height synchronization, inject 1 time, about make
The ewe estrus synchronization of 60%-70%, the 2nd injection are separated by 8-9 days, are handled 7-9 days with progestin preparation, before ending processing
1 day, 1 prostaglandin of intramuscular injection；
(7) embryo for collecting step (5) carries out embryo transfer to the sheep ewe after step (6) estrus synchronization, fertilized eggs
It is transplanted to fallopian tubal with mulberry body, does a kerf in sheep ewe abdomen, find the ovary of ovulation side and observes corpus luteum development
Situation, embryo, which is injected into ipsilateral cornua uteri upper end or ampulla of uterine tube, with syringe or grafts need to only take a small amount of rush
Washing lotion connection embryo injects together.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109589184A (en) * | 2019-01-18 | 2019-04-09 | 海南职业技术学院 | A kind of sequencing semen deposition method of black goat |
| CN110214744A (en) * | 2019-05-28 | 2019-09-10 | 临沂市农业科学院 | A kind of Yimeng black goat purification and rejuvenation method |
| CN113180878A (en) * | 2021-04-23 | 2021-07-30 | 广西壮族自治区畜牧研究所 | Method for improving double-birth rate of cows |
-
2018
- 2018-07-27 CN CN201810845158.7A patent/CN109090025A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109589184A (en) * | 2019-01-18 | 2019-04-09 | 海南职业技术学院 | A kind of sequencing semen deposition method of black goat |
| CN110214744A (en) * | 2019-05-28 | 2019-09-10 | 临沂市农业科学院 | A kind of Yimeng black goat purification and rejuvenation method |
| CN113180878A (en) * | 2021-04-23 | 2021-07-30 | 广西壮族自治区畜牧研究所 | Method for improving double-birth rate of cows |
| CN113180878B (en) * | 2021-04-23 | 2024-05-07 | 广西壮族自治区畜牧研究所 | Method for improving double-embryo rate of cows |
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