CN106137448A - A kind of method inducing high yield cow timing to ovulate and improve conception rate - Google Patents
A kind of method inducing high yield cow timing to ovulate and improve conception rate Download PDFInfo
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- CN106137448A CN106137448A CN201510127894.5A CN201510127894A CN106137448A CN 106137448 A CN106137448 A CN 106137448A CN 201510127894 A CN201510127894 A CN 201510127894A CN 106137448 A CN106137448 A CN 106137448A
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Abstract
A kind of method inducing high yield cow timing to ovulate and improve conception rate, 1. after high yield cow calving 35-40 days, at milch cow intravaginal heeling-in progestogen device first, 2. heeling-in took out progestogen device after 14-16 days, 3. inject prostaglandin, 4. after injection prostaglandin 2 days, inject ovulation rate hormone, 5. injection ovulation rate hormone after 12-16 hour, carry out artificial insemination；6. the 6-8 days after artificial insemination, again heeling-in progestogen device, the heeling-in time is 14-16 days again.The growth of follicle and ovulation period in this method controllable milch cow ovary, and it is made without detection of oestrus, directly carry out artificial insemination, use anti-abortion medicine simultaneously, improve the survival rate of embryo.The present invention not only overcomes high yield cow and is difficult to observe the defect oestrused, and is conducive to improving cow oestrus rate and conception rate simultaneously, shortens the calving interval of high yield cow, improve the culture benefit of high yield cow, it is simple to the production management of cattle farm.
Description
Technical field
The present invention relates to a kind of livestock reproduction method, especially a kind of induction high yield cow timing is ovulated and improves
The method of conception rate.
Background technology
At present, China's cow reproduction mainly uses technology of artificial insemination, compared with natural mating, uses artificial
Insemination techniques reducibleness pathophoresis, reduce reproductive-cost, frozen semen can storage and transport for a long time,
Female animal breeds without geographical restrictions, and can carry out international exchange and trade, the introduction of vicariad male animal.
When carrying out artificial insemination, it is necessary to carry out detection of oestrus accurately.At present, detection of oestrus is mainly by seeing
The performance of oestrusing examining or monitoring cow judges.But, along with improving constantly of milk production of cow, it is oestrused
Symptom is not the most notable, i.e. quiet estrus.According to investigations, quiet estrus account for oestrusing milch cow 10%-20% (
Gui Lan etc., 2012, Heilungkiang animal reproduction), owing to can not promptly and accurately judge to oestrus, thus miss and oestrus
Phase, it is impossible to semen deposition is bred, and significantly impact milch cow is joined rate, adds feeding cost and generation inteval,
Reduce the economic benefit of milk cattle cultivating.Therefore, research one induction high yield cow timing ovulation and raising are subject to
The method of tire rate, is to be presently required to solve the technical problem that.
Summary of the invention
The technical problem to be solved is to provide a kind of induction high yield cow timing ovulation and raising is impregnated
The method of rate.
The present invention solves the technical scheme of its technical problem:
A kind of induce high yield cow timing ovulation and the method improving conception rate, 1. after high yield cow calving 35
-40 days, at milch cow intravaginal heeling-in progestogen device first, 2. progestogen dress was taken out in heeling-in after 14-16 days
Put, 3. inject prostaglandin, 4. after injection prostaglandin 2 days, inject ovulation rate hormone, 5. inject rush
Enter after egg laying hormone 12-16 hour, carry out artificial insemination；6. the 6-8 days after artificial insemination, then
Secondary heeling-in progestogen device, the heeling-in time is 14-16 days again.
One as the present invention selects scheme, described progestogen device to be progestogen spongy embolus or CIDR.
One as the present invention selects scheme, described progestogen to be LNG, progesterone, methyl
Acetic acid progesterone, chlormadinone acetate, megestrol or norethindrone.
One as the present invention selects scheme, containing LNG in the progestogen spongy embolus of heeling-in first
50-100mg or containing the suitable progesterone of biological activity, methyl acetic acid progesterone (abbreviation medroxyprogesterone), acetic acid chlorine ground
Progesterone (abbreviation chlormadinone), megestrol or norethindrone；The CIDR of heeling-in first is containing progesterone 1.38g
Or contain the suitable LNG of biological activity, methyl acetic acid progesterone, chlormadinone acetate, megestrol
Or norethindrone.
One as the present invention selects scheme, again containing LNG in the progestogen spongy embolus of heeling-in
25-50mg or pregnant containing the suitable progesterone of biological activity, methyl acetic acid progesterone (abbreviation medroxyprogesterone), acetic acid chlorine ground
Ketone (abbreviation chlormadinone), megestrol or norethindrone；Again the CIDR of heeling-in be containing progesterone 0.69g or
Containing the suitable LNG of biological activity, methyl acetic acid progesterone, chlormadinone acetate, megestrol or
Norethindrone.
One as the present invention selects scheme, described injection prostaglandin to be intramuscular injection or intrauterine filling
Note.
As the one of the present invention select scheme, described prostaglandin be Cloprostenol, fluorine prostatitis enol,
PGF2 α, methyl PGF2 α or PGF1 α methyl ester.
One as the present invention selects scheme, and intramuscular injection Cloprostenol 0.4mg or biological activity are suitable
PGF2 α, fluorine prostatitis enol, methyl PGF2 α or PGF1 α methyl ester；Intrauterine infusion Cloprostenol
0.2mg or PGF2 α, fluorine prostatitis enol, methyl PGF2 α or PGF1 α methyl ester.
As the one of the present invention select scheme, described ovulation rate hormone be decorporation 3, decorporation 2,
Lutropin, human chorionic gonadotropin, gonadotropin releasing hormone.
One as the present invention selects scheme, injection decorporation 3 or No. 2 25-50 μ g of decorporation, rush Huang
Voxel 50-100IU, human chorionic gonadotropin 1000-2000IU or gonadotropin releasing hormone
100μg。
Compared with prior art, the inventive method after high yield cow calving 35-40 days, swashs by heeling-in is pregnant
Element spongy embolus or CIDR, spongy embolus or CIDR, injection prostaglandin and rush property are taken out in heeling-in after 14-16 days
Glandular hormone releasing hormone or the efficient analog of gonadotropin releasing hormone, make milch cow within the predetermined time
Oestrus, and some reproductive diseases of milch cow is also had certain therapeutical effect, promote oestrusing, no of milch cow
Need to carry out ' Oestrus Observation and monitoring, save man power and material.Instant invention overcomes current high yield cow to send out
Feelings performance is inconspicuous, and ovulation period individual variation is big, and milch cow First Trimester embryo loses the problems such as serious,
It is that a kind of new induction milch cow timing is oestrused and ovulates, then carries out artificial insemination, use maintaining pregnant measure simultaneously,
Reduce the integrated approach that embryo loses.
The principle of the present invention:
After progestogen spongy embolus or CIDR are placed into female animal vagina, contained progestogen slowly release, through the moon
Mucous membrane enters internal, makes progestogen in blood plasma keep valid density, as playing with endogenous progesterone
Effect, by negative feedback inhibition pituitary gonadotropic hormone and the secretion of hypothalamus gonadotropin releasing hormone,
Thus suppress estrus of cow and ovulation.Once taking-up progestogen spongy embolus or CIDR, the event resolves of progestogen,
The secretion of hypothalamus of animals gonadotropin releasing hormone increases, and pituitary gonadotropic hormone secretion increases simultaneously, promotees
Enter the growth of follicle and animals into heat (Li Yuhua and Wang Zhiqiang, 2009, herding and veterinary).The present invention utilizes
This mechanism of action, makes cow oestrus within the predetermined time.
The Main Function of gonadotropin releasing hormone is to promote the synthesis of anterior pituitary gonadotropin and release
Put, wherein main to promote that lutropin is released to, also there are the effect promoting follicle stimulating hormone release, lutropin
With the preovulatory Follicular growth of selective induction, and ovulation can be triggered.Human chorionic gonadotropin is raw
Thing effect is similar to lutropin, can promote follicle maturity and ovulation.Decorporation 3, decorporation 2 are to promote
The efficient analog of gonadotropin releasing hormone, its physiological function and gonadotropin releasing hormone are essentially identical.
Due to the difference that milch cow is individual, from there is the time interval showed ovulation of oestrusing, different milch cows are not
With, by injection gonadotropin releasing hormone or its efficient analog or lutropin or human chorionic
Film promoting sexual gland hormone, can strengthen the ovulation function of ovary, makes ovulation carry out within the predetermined time, then
Carry out artificial insemination within the predetermined time, add the probability of sperm ovum binding, and then improve conception rate.
The inventive method after taking out spongy embolus or CIDR the 2nd day, injection gonadotropin releasing hormone or it is efficient
Analog or lutropin or human chorionic gonadotropin, promote milch cow follicle maturity and ovulation.And
After 12-16 hour, carry out artificial insemination.
According to statistics, the average conception rate of cattle is 90%, but calving rate only has 55%, and embryo or foetal death account for
To 35%, wherein 70-80% embryo lose occur after breeding the 8-16 days (Spencer, 2013,
Animal Frontiers).Owing to embryo loses peak period precisely maternal gestational identification and the attached pass planted of embryo
The key phase, therefore embryo is by the immunologic rejection of parent, is the main cause causing embryonic death.Interferon tau
Being the primigravida signal that sends to parent of ruminant embryo, Bov IFN-tau is with paracrine form effect
In endometrium, make the biosynthesis block of estrogen receptor, cause the synthesis of ocytocin receptor to reduce, then make
The release of dinoprost is suppressed, and plays the effect of Antiluteolysis, makes the corpus luteum can be pregnant with continuous release
Ketone.It is reported, vaginal suppository supplements progesterone, can improve the pregnancy rate of human oocyte monosperm microinjection
(Manno etc., 2005, Fertility Sterility).The present invention, the 6-8 days after breeding,
Heeling-in progestogen spongy embolus or CIDR, supplemented with exogenous progestogen, reach to improve the purpose of milch cow pregnancy rate.
Detailed description of the invention
A kind of method inducing high yield cow timing to ovulate and improve conception rate, specifically comprises the following steps that
1. after high yield cow calving 35-40 days, heeling-in progestogen spongy embolus (LNG 50
-100mg, or the suitable progesterone of biological activity, methyl acetic acid progesterone (abbreviation medroxyprogesterone), acetic acid chlorine ground is pregnant
Ketone (abbreviation chlormadinone), megestrol or norethindrone) or CIDR (progesterone 1.38g, or containing biological activity
Suitable LNG, methyl acetic acid progesterone, chlormadinone acetate, megestrol or norethindrone).
2. heeling-in took out spongy embolus or CIDR after 14-16 days, then injected prostaglandin (intramuscular injection state
Produce Cloprostenol 0.4mg or the suitable PGF2 α of biological activity, fluorine prostatitis enol, methyl PGF2 α or
PGF1 α methyl ester；Or intrauterine infusion domestic Cloprostenol 0.2mg, or the suitable PGF2 α of biological activity,
Fluorine prostatitis enol, methyl PGF2 α or PGF1 α methyl ester)；Injection prostaglandin, after 2 days, injects decorporation 3
Number or No. 2 25-50 μ g or lutropin 50-100IU of decorporation or human chorionic gonadotropin 1000
-2000IU or gonadotropin releasing hormone 100 μ about g；After 12-16 hour, carry out artificial
Insemination.
3. the 6-8 days after breeding, and heeling-in progestogen spongy embolus (LNG 25-50mg, or raw
The active suitable progesterone of thing, methyl acetic acid progesterone (abbreviation medroxyprogesterone), chlormadinone acetate (are called for short chlorine ground pregnant
Ketone), megestrol or norethindrone) or CIDR (progesterone 0.69g, or or containing the suitable 18-first of biological activity
Base norethindrone, methyl acetic acid progesterone, chlormadinone acetate, megestrol or norethindrone), the heeling-in time is 14
-16 days.
Claims (10)
1. induce high yield cow timing ovulation and the method improving conception rate, 1. after high yield cow calving
35-40 days, at milch cow intravaginal heeling-in progestogen device first, 2. heeling-in took out progestogen after 14-16 days
Device, 3. injects prostaglandin, 4. after injection prostaglandin 2 days, injects ovulation rate hormone, 5. injects rush
Enter after egg laying hormone 12-16 hour, carry out artificial insemination；6. the 6-8 days after artificial insemination, again
Heeling-in progestogen device, the heeling-in time is 14-16 days again.
A kind of method inducing high yield cow timing to ovulate and improve conception rate the most according to claim 1,
It is characterized in that, described progestogen device is progestogen spongy embolus or CIDR.
A kind of side inducing high yield cow timing ovulation and improving conception rate the most according to claim 2
Method, it is characterised in that described progestogen are LNG, progesterone, methyl acetic acid progesterone, acetic acid
Chlormadinone, megestrol or norethindrone.
A kind of method inducing high yield cow timing to ovulate and improve conception rate the most according to claim 3,
It is characterized in that, containing LNG 50-100mg or containing biology in the progestogen spongy embolus of heeling-in first
The suitable progesterone of activity, methyl acetic acid progesterone (abbreviation medroxyprogesterone), chlormadinone acetate (abbreviation chlormadinone),
Megestrol or norethindrone；The CIDR of heeling-in first is containing progesterone 1.38g or to contain the 18-that biological activity is suitable
Methylnorethindron, methyl acetic acid progesterone, chlormadinone acetate, megestrol or norethindrone.
A kind of method inducing high yield cow timing to ovulate and improve conception rate the most according to claim 3,
It is characterized in that, again containing LNG 25-50mg or containing biology in the progestogen spongy embolus of heeling-in
The suitable progesterone of activity, methyl acetic acid progesterone (abbreviation medroxyprogesterone), chlormadinone acetate (abbreviation chlormadinone),
Megestrol or norethindrone；The CIDR of heeling-in is containing progesterone 0.69g or to contain the 18-that biological activity is suitable again
Methylnorethindron, methyl acetic acid progesterone, chlormadinone acetate, megestrol or norethindrone.
A kind of method inducing high yield cow timing to ovulate and improve conception rate the most according to claim 1,
It is characterized in that, described injection prostaglandin is intramuscular injection or intrauterine infusion.
A kind of method inducing high yield cow timing to ovulate and improve conception rate the most according to claim 1,
It is characterized in that, described prostaglandin is Cloprostenol, fluorine prostatitis enol, PGF2 α, methyl PGF2
α or PGF1 α methyl ester.
A kind of method inducing high yield cow timing to ovulate and improve conception rate the most according to claim 7,
It is characterized in that, intramuscular injection Cloprostenol 0.4mg or the suitable PGF2 α of biological activity, fluorine prostalene
Alcohol, methyl PGF2 α or PGF1 α methyl ester；Intrauterine infusion Cloprostenol 0.2mg or PGF2 α, fluorine
Prostatitis enol, methyl PGF2 α or PGF1 α methyl ester.
A kind of method inducing high yield cow timing to ovulate and improve conception rate the most according to claim 1,
It is characterized in that, described ovulation rate hormone is decorporation 3, decorporation 2, lutropin, human chorionic
Promoting sexual gland hormone, gonadotropin releasing hormone.
A kind of side inducing high yield cow timing ovulation and improving conception rate the most according to claim 9
Method, it is characterised in that injection decorporation 3 or No. 2 25-50 μ g of decorporation, lutropin 50-100IU,
Human chorionic gonadotropin 1000-2000IU or gonadotropin releasing hormone 100 μ g.
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| CN107099501A (en) * | 2017-05-27 | 2017-08-29 | 华南农业大学 | Method for inducing young primates to obtain gonad gametes and application |
| CN107648593A (en) * | 2017-09-30 | 2018-02-02 | 新疆豪子畜牧业有限公司 | A kind of preparation for being used to induce cow ovum chromosomal variation |
| CN107723272A (en) * | 2017-09-19 | 2018-02-23 | 武汉轻工大学 | A kind of preparation method of cattle early embryo Trophoblast tissue |
| CN109419565A (en) * | 2017-08-30 | 2019-03-05 | 周建标 | A kind of cow artificial insemination method |
| CN109431647A (en) * | 2018-09-20 | 2019-03-08 | 华中农业大学 | A kind of processing method of hybridization buffalo estrus synchronization-timing semen deposition |
| CN109589184A (en) * | 2019-01-18 | 2019-04-09 | 海南职业技术学院 | A kind of sequencing semen deposition method of black goat |
| CN110151354A (en) * | 2018-01-12 | 2019-08-23 | 塔里木大学 | One kind making the oestrus increased amount of method of Sheep Ovary advantage ovarian follicle |
| CN111329990A (en) * | 2020-02-28 | 2020-06-26 | 北京市农林科学院 | Method for improving conception rate of folliculofloxacin cows |
| CN114948325A (en) * | 2021-12-31 | 2022-08-30 | 江苏省农业科学院 | Follicle development treatment method for improving cow mating conception rate |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107099501B (en) * | 2017-05-27 | 2022-04-05 | 广东华农大资产经营有限公司 | Method for inducing young primates to obtain gonad gametes and application |
| CN107099501A (en) * | 2017-05-27 | 2017-08-29 | 华南农业大学 | Method for inducing young primates to obtain gonad gametes and application |
| CN109419565A (en) * | 2017-08-30 | 2019-03-05 | 周建标 | A kind of cow artificial insemination method |
| CN107723272B (en) * | 2017-09-19 | 2021-05-18 | 武汉轻工大学 | Method for obtaining cattle early embryo trophoblast tissue |
| CN107723272A (en) * | 2017-09-19 | 2018-02-23 | 武汉轻工大学 | A kind of preparation method of cattle early embryo Trophoblast tissue |
| CN107648593A (en) * | 2017-09-30 | 2018-02-02 | 新疆豪子畜牧业有限公司 | A kind of preparation for being used to induce cow ovum chromosomal variation |
| CN110151354A (en) * | 2018-01-12 | 2019-08-23 | 塔里木大学 | One kind making the oestrus increased amount of method of Sheep Ovary advantage ovarian follicle |
| CN109431647A (en) * | 2018-09-20 | 2019-03-08 | 华中农业大学 | A kind of processing method of hybridization buffalo estrus synchronization-timing semen deposition |
| CN109431647B (en) * | 2018-09-20 | 2020-10-30 | 华中农业大学 | Processing method for simultaneous estrus and timing semen deposition of hybrid buffalo |
| CN109589184A (en) * | 2019-01-18 | 2019-04-09 | 海南职业技术学院 | A kind of sequencing semen deposition method of black goat |
| CN111329990A (en) * | 2020-02-28 | 2020-06-26 | 北京市农林科学院 | Method for improving conception rate of folliculofloxacin cows |
| CN111329990B (en) * | 2020-02-28 | 2022-05-24 | 北京市农林科学院 | Method for improving conception rate of folliculofloxacin cows |
| CN114948325A (en) * | 2021-12-31 | 2022-08-30 | 江苏省农业科学院 | Follicle development treatment method for improving cow mating conception rate |
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