CN110151354A - One kind making the oestrus increased amount of method of Sheep Ovary advantage ovarian follicle - Google Patents
One kind making the oestrus increased amount of method of Sheep Ovary advantage ovarian follicle Download PDFInfo
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- CN110151354A CN110151354A CN201810029809.5A CN201810029809A CN110151354A CN 110151354 A CN110151354 A CN 110151354A CN 201810029809 A CN201810029809 A CN 201810029809A CN 110151354 A CN110151354 A CN 110151354A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
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Abstract
The invention discloses one kind to make the oestrus increased amount of method of Sheep Ovary advantage ovarian follicle, comprising the following steps: (1) selection of sheep；(2) prepare reagent；(3) vagina CIDR bolt is handled with sterile saline, after being then placed in 30-45 DEG C of water-bath warm bath for a period of time, carries out the operation of heeling-in vagina CIDR bolt to sheep；(4) FSH 60-80IU//times are respectively injected the 11st day morning, afternoon in heeling-in；Respectively inject FSH 30-40IU//times the 12nd day morning, afternoon；The 13rd day injection FSH morning, afternoon respectively inject FSH 10-20IU//times, and inject chlorine gas system PG 0.2mg while injection hypophysis luteotropin LH 100-150IU, the 13rd day P.M. injection FSH while injecting FSH in the 13rd day morning.Method of the invention can effectively increase oestrus Sheep Ovary advantage ovarian follicle quantity, and operating method is simple, be conducive to allow more collected, the ovarian follicle that selects grows into advantage ovarian follicle.
Description
Technical field
It is specifically a kind of to keep oestrus Sheep Ovary advantage ovarian follicle increased amount of the present invention relates to field of medical technology
Method.
Background technique
Sheep is monotocous animal, and reproductive capacity greatly limits the performance of productivity.Under natural conditions, ewe
Contain about 300,000 or so ovarian follicles in ovary, but only have 1% in natural conditions to reach maturity and ovulate, about 99% ovum
Generation locking of steeping oneself-meeting is degenerated.With the development of science and technology, allow more collected, the ovarian follicle that selects grows into advantage ovarian follicle,
This problem can be broken off relations to a certain extent, induced in ewe ovary using exogenous gonadotrophin and collected, selected
Ovarian follicle advantage, and the method finally reached maturity and the ovum with fertility is discharged.
Summary of the invention
The purpose of the present invention is to provide one kind to make the oestrus increased amount of method of Sheep Ovary advantage ovarian follicle, with solution
Certainly the problems mentioned above in the background art.
To achieve the above object, the invention provides the following technical scheme:
One kind making the oestrus increased amount of method of Sheep Ovary advantage ovarian follicle, comprising the following steps:
(1) selection of sheep: choose it is healthy, disease-free, without pregnant sheep；
(2) prepare reagent: vagina CIDR bolt, hypophysis follicle-stimulating hormone FSH, hypophysis luteotropin LH and chlorine gas system PG；
(3) vagina CIDR bolt is handled with sterile saline, right after being then placed in 30-45 DEG C of water-bath warm bath for a period of time
Sheep carries out the operation of heeling-in vagina CIDR bolt；
(4) FSH 60-80IU//times are respectively injected the 11st day morning, afternoon in heeling-in, the midfeather 10-15 of double injection is small
When；Respectively inject FSH 30-40IU//times the 12nd day morning, afternoon, the midfeather of double injection 10-15 hours；13rd day
Injection the FSH morning, afternoon respectively inject FSH 10-20IU/ only/time, the midfeather of double injection 10-15 hours, and the 13rd
Chlorine is injected while injection hypophysis luteotropin LH 100-150IU, the 13rd day P.M. injection FSH while its morning injects FSH
Forefront dilute alcohol PG 0.2mg.
As a further solution of the present invention: step (3) vagina CIDR bolt is handled with sterile saline, is then placed within
In 38 DEG C of water-baths warm bath for a period of time after, to sheep carry out heeling-in vagina CIDR bolt operation.
As a further solution of the present invention: step (4) is in the 11st day morning of heeling-in, the midfeather of double injection in afternoon
12 hours；The 12nd day morning, midfeather 12 hours of double injection in afternoon；The 13rd day injection FSH morning, double injection in afternoon
Midfeather 12 hours.
Compared with prior art, the beneficial effects of the present invention are:
Method of the invention can effectively increase oestrus Sheep Ovary advantage ovarian follicle quantity, and operating method is simple, is conducive to allow
The ovarian follicle more collected, selected grows into advantage ovarian follicle, is a kind of application exogenous gonadotrophin induction ewe ovary
The middle ovarian follicle advantage collected, selected, and the method finally reached maturity and the ovum with fertility is discharged.
Specific embodiment
The technical solution of the patent is explained in further detail With reference to embodiment.
Embodiment 1
One kind making the oestrus increased amount of method of Sheep Ovary advantage ovarian follicle, comprising the following steps: (1) selection of sheep:
Choose it is healthy, disease-free, without pregnant 2 years old sheep；(2) prepare reagent: vagina CIDR bolt, hypophysis follicle-stimulating hormone FSH, hypophysis luteotropin
LH and chlorine gas system PG；(3) vagina CIDR bolt is handled with sterile saline, is then placed in 30 DEG C of water-baths one section of warm bath
After time, the operation of heeling-in vagina CIDR bolt is carried out to sheep；(4) FSH 60IU/ is respectively injected the 11st day morning, afternoon in heeling-in
Only/time, the midfeather of double injection 10 hours；Respectively inject FSH 30IU//times the 12nd day morning, afternoon, double injection
Midfeather 10 hours；The 13rd day injection FSH morning, afternoon respectively inject FSH 10IU//times, the midfeather of double injection
10 hours, and hypophysis luteotropin LH 100IU, the 13rd day P.M. injection FSH are injected while injecting FSH in the 13rd day morning
While inject chlorine gas system PG 0.2mg.
Embodiment 2
One kind making the oestrus increased amount of method of Sheep Ovary advantage ovarian follicle, comprising the following steps: (1) selection of sheep:
Choose it is healthy, disease-free, without pregnant 3 years old sheep；(2) prepare reagent: vagina CIDR bolt, hypophysis follicle-stimulating hormone FSH, hypophysis luteotropin
LH and chlorine gas system PG；(3) vagina CIDR bolt is handled with sterile saline, is then placed in 45 DEG C of water-baths one section of warm bath
After time, the operation of heeling-in vagina CIDR bolt is carried out to sheep；(4) FSH 80IU/ is respectively injected the 11st day morning, afternoon in heeling-in
Only/time, the midfeather of double injection 15 hours；Respectively inject FSH 40IU//times the 12nd day morning, afternoon, double injection
Midfeather 15 hours；The 13rd day injection FSH morning, afternoon respectively inject FSH 20IU//times, the midfeather of double injection
15 hours, and hypophysis luteotropin LH 150IU, the 13rd day P.M. injection FSH are injected while injecting FSH in the 13rd day morning
While inject chlorine gas system PG 0.2mg.
Embodiment 3
One kind making the oestrus increased amount of method of Sheep Ovary advantage ovarian follicle, comprising the following steps: (1) selection of sheep:
Choose it is healthy, disease-free, without pregnant 2 years old sheep；(2) prepare reagent: vagina CIDR bolt, hypophysis follicle-stimulating hormone FSH, hypophysis luteotropin
LH and chlorine gas system PG；(3) vagina CIDR bolt is handled with sterile saline, is then placed in 38 DEG C of water-baths one section of warm bath
After time, the operation of heeling-in vagina CIDR bolt is carried out to sheep；(4) FSH 70IU/ is respectively injected the 11st day morning, afternoon in heeling-in
Only/time, the midfeather of double injection 12 hours；Respectively inject FSH 35IU//times the 12nd day morning, afternoon, double injection
Midfeather 12 hours；The 13rd day injection FSH morning, afternoon respectively inject FSH 15IU//times, the midfeather of double injection
12 hours, and hypophysis luteotropin LH 120IU, the 13rd day P.M. injection FSH are injected while injecting FSH in the 13rd day morning
While inject chlorine gas system PG 0.2mg.
Experimental example
One, experimental animal: health, more unrestrained sheep ewe 30 without disease are randomly divided into control group and (every group each 15 of processing group
Only)；
Two, reagent: vagina CIDR bolt, hypophysis follicle-stimulating hormone FSH, hypophysis luteotropin LH and chlorine gas system PG are from Ningbo three
Raw pharmaceutcal corporation, Ltd's purchase；
Three, control group (CIDR+PG) and processing group (CIDR+FSH+LH+PG) processing test method: are carried out.Wherein:
Control group method: vagina CIDR bolt is sealed after being processed with sterile saline and is placed, and 38 DEG C of water-baths are placed on before use
Middle warm bath carries out the operation of heeling-in vagina CIDR bolt for a period of time, to sheep；Sheep unrestrained more than 15 is used in experimental period any one day
The disposable heeling-in of New Zealand's vagina CIDR bolt, 13 days recession bolts of heeling-in, and intramuscular injection chlorine gas system PG0.2mg；From heeling-in
Every afternoon is butchered observation folliculus ovarii developmental condition in beginning in 10th day；
Processing group method: vagina CIDR bolt is handled with sterile saline, is then placed in 38 DEG C of water-baths warm bath for a period of time
Afterwards, the operation of heeling-in vagina CIDR bolt is carried out to sheep；Heeling-in respectively inject the 11st day morning, afternoon FSH 70IU/ only/time, two
The midfeather of secondary injection 12 hours；Respectively inject FSH 35IU//times the 12nd day morning, afternoon, the midfeather of double injection
12 hours；The 13rd day injection FSH morning, afternoon respectively inject FSH 15IU/ only/time, the midfeather of double injection 12 hours, and
It is injected while injection hypophysis luteotropin LH 120IU, the 13rd day P.M. injection FSH while injecting FSH in the 13rd day morning
Chlorine gas system PG 0.2mg；And it is the same with control group, ovary is excellent after afternoon is butchered observation HORMONE TREATMENT in heeling-in beginning in the 10th day
Change ovarian follicle quantity；
Four, data are analyzed, and test the data obtained uses mean+SD SD；Ovarian follicle quantity is shown in Table 1 and table 2；
TableProcessing group handles different number of days folliculus ovarii quantity
2 control group of table handles different number of days folliculus ovarii quantity
It is obvious to can be seen that processing group and the control group difference in number from table 1-2.The 11st day morning of processing group injects the first needle
Promoting sexual gland hormone, interval are butchered for 10 hours, and quantity processing group of the follicular diameter greater than 3mm 3 left sides more than control group can be observed
It is right.Processing group is under foreign aid's hormone FSH effect, and Ovarian surface ovarian follicle quantity progressively increases to be declined slightly after 13d, this is perhaps
Competitive growth between ovarian follicle is related, especially 15d, after having follicular rupture ovulation, under progestational hormone can feed back to signal
Thalamus, the amount of the FSH and LH that synthesize hypothalamus are opposite to be reduced, and the ovarian follicle in some growth, which is suppressed, to be even latched.
And pass through the morphological analysis to ovary tissue, it is known that: since 12d, the growth of processing group Ovarian surface ovarian follicle
Quickly, ovarian follicle wall is thinning for speed, and the prominent surface of ovarian follicle quantity hemispherical obviously increases, and handles 13d, 14d, Ovarian surface
There is transparent round ovulation points amount to obviously increase than control group；15d advantageousization follicular rupture ovulation, ovulation
Afterwards, and it is visible in the corpus hemorrhagicum of volcanic crater sample in breach.
The preferred embodiment of the patent is described in detail above, but this patent is not limited to above-mentioned embodiment party
Formula within the knowledge of one of ordinary skill in the art can also be under the premise of not departing from this patent objective
Various changes can be made.
Claims (3)
1. one kind makes the oestrus increased amount of method of Sheep Ovary advantage ovarian follicle, which comprises the following steps:
(1) selection of sheep: choose it is healthy, disease-free, without pregnant sheep；
(2) prepare reagent: vagina CIDR bolt, hypophysis follicle-stimulating hormone FSH, hypophysis luteotropin LH and chlorine gas system PG；
(3) vagina CIDR bolt is handled with sterile saline, right after being then placed in 30-45 DEG C of water-bath warm bath for a period of time
Sheep carries out the operation of heeling-in vagina CIDR bolt；
(4) FSH 60-80IU//times are respectively injected the 11st day morning, afternoon in heeling-in, the midfeather 10-15 of double injection is small
When；Respectively inject FSH 30-40IU//times the 12nd day morning, afternoon, the midfeather of double injection 10-15 hours；13rd day
Injection the FSH morning, afternoon respectively inject FSH 10-20IU/ only/time, the midfeather of double injection 10-15 hours, and the 13rd
Chlorine is injected while injection hypophysis luteotropin LH 100-150IU, the 13rd day P.M. injection FSH while its morning injects FSH
Forefront dilute alcohol PG 0.2mg.
2. according to claim 1 make the oestrus increased amount of method of Sheep Ovary advantage ovarian follicle, which is characterized in that
Step (3) vagina CIDR bolt is handled with sterile saline, after being then placed in 38 DEG C of water-baths warm bath for a period of time, to sheep
Carry out the operation of heeling-in vagina CIDR bolt.
3. according to claim 1 make the oestrus increased amount of method of Sheep Ovary advantage ovarian follicle, which is characterized in that
Step (4) was in the 11st day morning of heeling-in, midfeather 12 hours of double injection in afternoon；12nd day morning, double injection in afternoon
Midfeather 12 hours；The 13rd day injection FSH morning, midfeather 12 hours of double injection in afternoon.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810029809.5A CN110151354A (en) | 2018-01-12 | 2018-01-12 | One kind making the oestrus increased amount of method of Sheep Ovary advantage ovarian follicle |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810029809.5A CN110151354A (en) | 2018-01-12 | 2018-01-12 | One kind making the oestrus increased amount of method of Sheep Ovary advantage ovarian follicle |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1919158A (en) * | 2006-09-13 | 2007-02-28 | 北京锦绣大地农业股份有限公司 | Cattle continuous processes method |
| CN102068322A (en) * | 2011-02-22 | 2011-05-25 | 安徽农业大学 | Superovulation method for goats |
| CN103610517A (en) * | 2013-12-04 | 2014-03-05 | 内蒙古农业大学 | Alxa bactrian camel superovulation method |
| CN106137448A (en) * | 2015-03-19 | 2016-11-23 | 河北工程大学 | A kind of method inducing high yield cow timing to ovulate and improve conception rate |
| US20170095536A1 (en) * | 2015-10-05 | 2017-04-06 | Ostara Biomedical Ltd | Methods and compositions for managing reproduction |
-
2018
- 2018-01-12 CN CN201810029809.5A patent/CN110151354A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1919158A (en) * | 2006-09-13 | 2007-02-28 | 北京锦绣大地农业股份有限公司 | Cattle continuous processes method |
| CN102068322A (en) * | 2011-02-22 | 2011-05-25 | 安徽农业大学 | Superovulation method for goats |
| CN103610517A (en) * | 2013-12-04 | 2014-03-05 | 内蒙古农业大学 | Alxa bactrian camel superovulation method |
| CN106137448A (en) * | 2015-03-19 | 2016-11-23 | 河北工程大学 | A kind of method inducing high yield cow timing to ovulate and improve conception rate |
| US20170095536A1 (en) * | 2015-10-05 | 2017-04-06 | Ostara Biomedical Ltd | Methods and compositions for managing reproduction |
Non-Patent Citations (3)
| Title |
|---|
| 密国辉 等: "贵州黑山羊超数排卵技术方案研究", 《广东农业科学》 * |
| 张金山 等: "不同处理方法对新疆褐牛同期发情效果的影响", 《现代农业科技》 * |
| 王惠娥 等: "春季卡拉库尔羊和多浪羊的超数排卵研究", 《江苏农业科学》 * |
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Application publication date: 20190823 |