CN109222956A - A kind of implanted light stimulus and the integrated flexible nerve electrode of electrographic recording - Google Patents
A kind of implanted light stimulus and the integrated flexible nerve electrode of electrographic recording Download PDFInfo
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- CN109222956A CN109222956A CN201811003971.6A CN201811003971A CN109222956A CN 109222956 A CN109222956 A CN 109222956A CN 201811003971 A CN201811003971 A CN 201811003971A CN 109222956 A CN109222956 A CN 109222956A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/24—Detecting, measuring or recording bioelectric or biomagnetic signals of the body or parts thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
- A61N5/00—Radiation therapy
- A61N5/06—Radiation therapy using light
- A61N5/0613—Apparatus adapted for a specific treatment
- A61N5/0622—Optical stimulation for exciting neural tissue
Abstract
The invention discloses the flexible nerve electrodes that a kind of light stimulus in the Technology of Microelectrodes field for belonging to neural engineering and brain science and electrographic recording are integrated.The electrode has package flexible, electrode outer layer has elasticity modulus similar with brain tissue, the mechanical damage to brain tissue, and the long-term availability since electrode cladding material has good biocompatibility, after promoting electrode implantation are reduced in electrode implantation process；PEDOT/PSS/MWCNT nerve electrode decorative material is added in electrode, there is lower impedance and good C-V characteristic, its electric property and biology performance can be significantly improved.
Description
Technical field
The invention belongs to the Technology of Microelectrodes fields of neural engineering and brain science, and in particular to a kind of light stimulus and electrographic recording
Integrated flexible nerve electrode and preparation method thereof.
Background technique
Light genetic technique provides a kind of research means for becoming individual character, it can be achieved that specific type neuron is living for Neuscience
Dynamic regulation.In light genetic research, optoelectronic pole plays the role of for light stimulus being transmitted to target area, and pushes away in research in recent years
Under dynamic, it is multi-functional the electric signal record for being provided simultaneously with light stimulus region, drug delivery etc. have been developed.Initially, the master of optoelectronic pole
Wanting form is that optical fiber and fibril electrode integration record neuron electric signal (Alexexai V while realizing light stimulus
Kiavite, Keita Tamura etc.), basic light genetics experiments demand can be met, but the function of electrode and spatial accuracy are equal
Generally.With Development of micro-fabrication technology, people start to be made neural photoelectricity of the technologies such as chemical meteorology deposition (CVD), photoetching
Pole (Euisik Yoon etc., Eric G.R, Fan Wu), these optoelectronic poles have more preferably on spatial positioning accuracy and resolution ratio
Performance.
In recent years, optoelectronic pole research focuses more on the multifunctionality, mechanical performance and biocompatibility of electrode.Optoelectronic pole
Multifunctionality be mainly reflected in light stimulus recorded with electric signal it is synchronous.2015, Joonhee Lee etc. was with transparent semiconductor
ZnO is material, is prepared for the photoelectrode array for having multi-channel light stimulation Yu electric signal writing function, and have very high space
Precision.2015, Andres Canales etc., which has studied one kind, can carry out high-resolution light stimulus, electrographic recording to movable mouse
With the fiber electrode of drug delivery, which still has good mechanical, electricity, light and micro-fluid measurement in bending deformation
Function.
Studies have shown that the mechanical performance of implanted electrode be cause the immune response of chronic implantation tissues following MCAO in rats it is important because
Element.For this purpose, more researchers are concerned with how to prepare optoelectronic pole flexible, to promote the long-term effectiveness of electrode.Implantation electricity
There are many method of pole flexibility, and can be used, itself there is material flexible to make electrode basement, and C. H. Chen et al. is used
Graphene optoelectronic pole of the SU-8 glue as substrate, can random 90-degree bent.Dimethyl silicone polymer (PDMS) is also common one
Micro- LED is precisely fitted in PDMS substrate by the base material of the flexible implant electrode of kind, J. W. Jeong et al., and in substrate
The channel of upper design heating administration, light stimulus is combined with administration and is prepared for a kind of flexible, good optoelectronic pole of biocompatibility.
Also research wraps up the flexibility that one layer of flexible material increases electrode entirety in surrounding them, Szu-Te Lin in 2011 et al. uses V
The optoelectronic pole that the method for slot capillary assembly is prepared for a deep brain light stimulus and gathers, by wrapping up flexibility outside Nitinol
PDMS has prepared light stimulus electrode flexible, and can not crack in tissue insertion and for a long time research.2015
Year, Sung Il Park et al. wraps up LED and radio-circuit by polyimides flexible (PI) and PDMS material, has developed soft
Property wireless implanted photoelectricity electrode systems, the light stimulus for realizing mouse periphery and spinal nerve is adjusted, for light genetic technique
Chronic clinical application be of great significance.
The biocompatibility of optoelectronic pole is another important indicator.2015, Joonhee Lee etc. was with transparent semiconductor
ZnO is material, is prepared for the photoelectrode array for having multi-channel light stimulation Yu electric signal writing function, is carried out at the tip of ZnO
The modification of ITO material, improves the biocompatibility at signal acquisition interface.In addition, Signa Gel is as a kind of with good soft
Property, biocompatibility, electric conductivity material have the advantages of highly significant in terms of modifying nerve electrode.Rylie A. Green etc.
People is combined using polyvinyl alcohol hydrogel and conductive polymer poly 3,4-rthylene dioxythiophene (PEDOT), using the side of electropolymerization
Method is prepared for a kind of and close electrode flexibility modifier of brain tissue pattern modulus, has important meaning in flexible electrode modification field
Justice.
Optoelectronic pole research is reported based on the fibril electrode integrated with optical fiber mostly at present, organizes invasive larger, optical fiber
Rigidity be more such that in be chronically implanted during electrode be easy biggish damage is caused to tissue, it is anti-so as to cause immune inflammation
The aggravation answered finally makes electrode failure.Existing research have developed cortical surface flexible material electrode for photoelectric synchronous stimulation and
Load, but not yet studies have reported that optoelectronic pole in a kind of cortex flexible, to realize the chronic smooth genetics research in cortex.It is soft
Property intrusive mood electrode can largely reduce the mechanical damage to brain tissue, to promote long-term, the stabilization signal of electrode
Acquisition capacity, it is extremely important for the validity of optoelectronic pole.Meanwhile the signal of electrode can be effectively promoted to the modification of electrode surface
Acquisition capacity.Research shows that the implant electrode on fluff material surface can reduce the immunological rejection of brain tissue, polyvinyl alcohol/
Multi-walled carbon nanotube (PVA/MWCNT) gel is since its good biocompatibility and mechanical performance are in terms of nerve electrode modification
With huge application potential, but its have as a kind of application performance of nerve electrode decorative material flexible it is to be excavated.
Summary of the invention
The purpose of the present invention is to provide a kind of flexible nerve electrode for being provided simultaneously with light stimulus and electrographic recording function and its
Preparation method, the electrode have package flexible, help to reduce after electrode is implanted into the mechanical damage of brain tissue to be promoted
The biocompatibility of electrode and long-term availability；Poly- 3,4- ethene dioxythiophene/polystyrolsulfon acid/multi wall carbon is added in electrode
Nanotube (PEDOT/PSS/MWCNT) nerve electrode decorative material, can significantly improve its electric property and biology performance.
To achieve the above object, the present invention is achieved through the following technical solutions:
The flexible nerve electrode that the implanted light stimulus and electrographic recording integrate is by electrode body, electric signal interface and optical fiber
Lock pin is constituted.
Wherein electrode body has transparent light guide substrate, hybrid conductive gel, electrode microfilament and support tungsten wire.Guide-lighting base
Signa Gel, support tungsten wire are wrapped in interior, one section of acicular electrode shape of formation by bottom；The hybrid conductive is gel-filled in electricity
In gap between atomic silk and guide-lighting substrate；The electrode microfilament extends a distance in electrode body rear end, and with electricity
The stitch of signaling interface welds conducting.The outer diameter of electrode body matches just with the internal diameter of fiber stub.
Preferably, the preparation of the electrode includes the following steps:
S1: production electrode leaded light substrate
More tungsten wires are inserted in parallel into a glass capillary, make tungsten wire both ends be exposed on the outside of capillary glass pipe end one section away from
From being affixed on glass pipe outer wall for equally distributed after this distance whole outward bending grown；By silica gel and curing agent (health
Peaceful sylgard184 model) mixing of 10:1 in mass ratio, removal air bubble is vacuumized after mixing evenly, is matched using syringe extraction
The PDMS completed is made, syringe needle tube is inserted into the bottom end for securing the glass capillary of tungsten wire and seals the two gap, slowly
Injector push-rod is pushed, in PDMS implantation glass pipe, will guarantee that PDMS is full of pipe internal pore；Glass capillary is sealed using AB glue
Lower end is to prevent PDMS from flowing out；Glass capillary is disposed vertically into 60 ° of ovens and is solidified, it is close to wipe out capillary after the completion of solidification
The bottom end of envelope stays a tungsten wire to keep the rigidity of electrode, extracts extra tungsten wire out and forms microchannel, forms the guide-lighting base of electrode
Bottom.
S2: preparation PEDOT/PSS/MWCNT electroplate liquid
MWCNT(0.2 mg/ml is added in pure water) through ultrasonic vibration to mixing well；It is successively added in mixed liquor later
EDOT(ethylene dibenzo thiophene, 0.1 wt%), PSS(polystyrolsulfon acid, 0.2 wt%), through ultrasonic vibration until mix well.
S3: electrode microfilament is prepared
A segment electrode microfilament is taken, is heated by alcolhol burner after removing its microfilament both ends surface insulation layer, one end is welded on electrode
On plug, the other end immerses in PEDOT/PSS/MWCNT electroplate liquid described in S2.By electrochemical workstation three-electrode system into
Row plating modification, using constant current anodizing process, polymerization electric current is 100nA, electroplating time 100s.Electrode microfilament is removed after plating.
S4: assembly electrode microfilament
By step described in S3, the preparation of more electrode microfilaments is completed, under microscopic visualization, the electrode microfilament that preparation is completed
In the microchannel for the guide-lighting substrate that insertion S1 completes one by one, guarantee to be inserted into a microfilament in each microchannel, microfilament plating
One end it is facing forward, microchannel front end reserves the about gap 5mm, and microfilament extends a segment length in microchannel rear end.
S5: preparation PVA/MWCNT Signa Gel
After the mixed liquor ultrasonic vibration of 0.5 mg/ml concentration MWCNT and pure water are mixed well, it is added with the weight proportion of 1:10
PVA and at 80 DEG C heating stirring until sufficiently dissolution mixing.
S6: perfusion Signa Gel
The made PVA/MWCNT Signa Gel got ready of S5 is extracted with syringe, the guide-lighting base of electrode microfilament will be assembled described in S4
Bottom front end protrudes into syringe needle tube and seals the two gap.Injector push-rod is slowly pushed, Signa Gel is filled into guide-lighting substrate
With the gap between electrode microfilament, guide-lighting substrate is unloaded after the completion and clears up sealing station, tentatively complete electrode body.
S7: freeze thawing is crosslinked Signa Gel
After the S6 electrode body completed is congealed at -20 DEG C, taking-up is melted at room temperature, is so alternately placed repeatedly, until
Signa Gel in electrode body is fully crosslinked.
S8: assembly electrode main body
The electrode body rear end that S7 is completed is inserted into fiber stub together with the more electrode microfilaments to extend out, electrode microfilament is worn
It is welded on electric signal interface after crossing fiber stub, the corresponding signal path of every microfilament；Separately take a naked layer not
The steel wire weld that becomes rusty is used as signal ground on electric signal interface.Electrode body is fixedly bonded on fiber stub using AB glue；With
Specific glue sealing electrode body both ends.
S9: etching removal electrode outer layer glass capillary
The electrode body leading portion that S8 is assembled is dipped vertically into hydrofluoric acid solution, until outer layer glass capillary is etched completely
It falls off, take out electrode and uses pure water rinsing.
S10: modified electrode tip
Shearing body front end is inclined-plane, makes electrode microfilament about 0.5mm at a distance from inclined-plane.It is carried out later by electrochemical workstation
Plating modification, electroplate liquid is the PEDOT/PSS/MWCNT electroplate liquid of preparation described in S2, using constant current anodizing process, three-electrode system,
Polymerization electric current is 100nA, electroplating time 100s.
S11: encapsulated electrode
With glue package microfilament and electric signal interface peripheral position, and by electric signal interface and fiber stub fixed bonding.Use PEG
The tip of the electrode of completion is electroplated described in encapsulation S10 and properly places.
Preferably, the diameter range of the electrode body is 200 ~ 360 μm, and length is 1 ~ 2cm.
Preferably, the electric signal interface, fiber stub are all made of standard component.
The utility model has the advantages that compared with existing implanted electrode, it is prepared by the present invention to be provided simultaneously with light stimulus and electrographic recording function
Can flexible nerve electrode, have lower impedance and good C-V characteristic, due to electrode outer layer have it is similar to brain tissue
Elasticity modulus, can reduce the mechanical damage to brain tissue in electrode implantation process, but due to electrode cladding material have it is good
Good biocompatibility, the long-term availability after promoting electrode implantation.
Detailed description of the invention
Fig. 1 is that the overall structure of the flexible nerve electrode of the present invention for having light stimulus and electrographic recording function is illustrated
Figure；
Fig. 2 is the electrode body longitudinal profile schematic diagram of the embodiment of the present invention；
Wherein 1- electrode body, 2- electric signal interface, 3- fiber stub, the bottom 21- contact pin, 131- tail end, 11- leaded light substrate,
12- hybrid conductive gel, 13- electrode microfilament, 14- support tungsten wire；
Fig. 3 is the preparation process schematic diagram of electrode body of embodiment of the present invention front end；
Fig. 4 is the impedance characteristic of the electrical performance testing of the embodiment of the present invention；
Fig. 5 is the electrical performance testing VA characteristic curve of the embodiment of the present invention.
Specific embodiment
The present invention is described in detail with reference to the accompanying drawings and examples, but implementation of the invention is not limited only to this.
As shown in Figure 1, the flexible nerve electrode that a kind of light stimulus and electrographic recording integrate, by electrode body 1, electric signal interface
2 and fiber stub 3 constitute.Wherein a segment electrode microfilament is extended in tail portion after electrode body, and the end 131 of electrode microfilament is logical
It crosses and welds the conducting that is connected with electric signal interface bottom contact pin 21；Internal diameter lucky of the outer diameter of electrode body 1 and fiber stub 3
Match, the back segment of electrode body 1 can be inserted into fiber stub, in the present embodiment, three is encapsulated as one by epoxide-resin glue
Body.
It is illustrated in figure 21 longitudinal profile schematic diagram of electrode body of the present embodiment, constituting includes transparent light guide substrate
11, hybrid conductive gel 12, electrode microfilament 13 and support tungsten wire 14.Signa Gel, support tungsten wire are wrapped in by guide-lighting substrate
It is interior, one section of acicular electrode shape is formed, between hybrid conductive gel 12 is filled between electrode microfilament 13 and guide-lighting substrate 11
In gap, electrode microfilament 13 extends a distance 131 for being connected with electric signal interface in body tail section.In the present embodiment
An electrode microfilament is contained, i.e. electrical signal paths all the way, but in different materials and technique preparation process, can be not limited to
This, may be comprising more parallel electrode microfilaments to form multi-channel electrode.
In conjunction with Fig. 3 electrode body front end preparation process schematic diagram, integrated soft of a kind of light stimulus of the present embodiment and electrographic recording
Property electrode preparation include the following steps:
S1: production electrode leaded light substrate
It is inserted in parallel into more tungsten wires (in Fig. 3 shown in a) in a glass capillary, tungsten wire both ends is made to be exposed to glass capillary
End outside a distance, is affixed on glass pipe outer wall for equally distributed after this distance whole outward bending grown；It will
PDMS two-component is mixed by 10:1, vacuumizes removal air bubble after mixing evenly, and the PDMS for preparing and completing is extracted using syringe,
Syringe needle tube is inserted into the bottom end for securing the glass capillary of tungsten wire and seals the two gap, slowly syringe is pushed to push away
Bar in PDMS implantation glass pipe, will guarantee that PDMS is full of pipe internal pore (in Fig. 3 shown in b)；Glass capillary is sealed using AB glue
Lower end is to prevent PDMS from flowing out；Glass capillary is disposed vertically into 60 ° of ovens and is solidified, it is close to wipe out capillary after the completion of solidification
The bottom end of envelope stays a tungsten wire to keep the rigidity of electrode, extracts extra tungsten wire out and forms microchannel (in Fig. 3 shown in c), forms electricity
The guide-lighting substrate of pole.
S2: poly- 3,4- ethene dioxythiophene/polystyrolsulfon acid/multi-walled carbon nanotube (PEDOT/PSS/MWCNT) is prepared
Electroplate liquid
MWCNT(0.2 mg/ml is added in pure water) through ultrasonic vibration to mixing well；It is successively added in mixed liquor later
EDOT(0.1 wt%), PSS(0.2 wt%), through ultrasonic vibration until mix well.
S3: electrode microfilament is prepared
A segment electrode microfilament is taken, is heated by alcolhol burner after removing its microfilament both ends surface insulation layer, one end is welded on electrode
On plug, the other end immerses in PEDOT/PSS/MWCNT electroplate liquid described in S2.By electrochemical workstation three-electrode system into
Row plating modification, using constant current anodizing process, polymerization electric current is 100nA, electroplating time 100s.Electrode microfilament is removed after plating.
S4: assembly electrode microfilament
By step described in S3, the preparation of more electrode microfilaments is completed, under microscopic visualization, the electrode microfilament that preparation is completed
In the microchannel for the guide-lighting substrate that insertion S1 completes one by one, guarantee to be inserted into a microfilament in each microchannel, microfilament plating
One end it is facing forward, microchannel front end reserves the gap about 5mm (in Fig. 3 shown in d), and microfilament extends one section long in microchannel rear end
Degree.
S5: preparation PVA/MWCNT Signa Gel
After the mixed liquor ultrasonic vibration of 0.5 mg/ml concentration MWCNT and pure water are mixed well, it is added with the weight proportion of 1:10
PVA and at 80 DEG C heating stirring until sufficiently dissolution mixing.
S6: perfusion Signa Gel
The made PVA/MWCNT Signa Gel got ready of S5 is extracted with syringe, the guide-lighting base of electrode microfilament will be assembled described in S4
Bottom front end protrudes into syringe needle tube and seals the two gap.Injector push-rod is slowly pushed, Signa Gel is filled into guide-lighting substrate
Gap (in Fig. 3 shown in e) between electrode microfilament, unloads guide-lighting substrate after the completion and clears up sealing station, tentatively made
At electrode body.
S7: freeze thawing is crosslinked Signa Gel
After the S6 electrode body completed is congealed at -20 DEG C, taking-up is melted at room temperature, is so alternately placed repeatedly, until
Signa Gel in electrode body is fully crosslinked.
S8: assembly electrode main body
The electrode body rear end that S7 is completed is inserted into fiber stub together with the more electrode microfilaments to extend out, electrode microfilament is worn
It is welded on electric signal interface after crossing fiber stub, the corresponding signal path of every microfilament；Separately take a naked layer not
The steel wire weld that becomes rusty is used as signal ground on electric signal interface.Electrode body is fixedly bonded on fiber stub using AB glue；With
Specific glue sealing electrode body both ends.
S9: etching removal electrode outer layer glass capillary (in Fig. 3 shown in f)
The electrode body leading portion that S8 is assembled is dipped vertically into hydrofluoric acid solution, until outer layer glass capillary is etched completely
It falls off, take out electrode and uses pure water rinsing.
S10: the modification of eletrode tip
Shearing body front end is inclined-plane, makes electrode microfilament about 0.5mm at a distance from inclined-plane.It is carried out later by electrochemical workstation
Plating modification (in Fig. 3 shown in g), electroplate liquid is the PEDOT/PSS/MWCNT electroplate liquid of preparation described in S2, using constant current
Method, three-electrode system, polymerization electric current are 100nA, electroplating time 100s.
S11: the encapsulation of electrode
With specific glue package microfilament and electric signal interface peripheral position, and by electric signal interface and fiber stub fixed bonding.
The tip of the electrode of completion is electroplated described in PEG encapsulation S10 and properly places, obtains the flexible nerve electrode.
Different according to the modification of eletrode tip, there is also difference for the electric property of electrode after the completion.The present embodiment passes through
Electrode preparation has been carried out using two kinds of CHI 660D type electrochemical workstation, PVA/MWCNT, PEDOT/PSS/MWCNT electroplate liquids,
And change the spacing between electrode microfilament and tip, electrical performance testing is carried out to the electrode of completion, impedance characteristic is aobvious
It is smaller apart from shorter impedance to show that eletrode tip retains, by the minimum (figure of electrode impedance after PEDOT/PSS/MWCNT plating modification
4)；Its VA characteristic curve is shown, most strong (Fig. 5) by the electrode storage charge capability after PEDOT/PSS/MWCNT plating modification.
The preferred embodiment of the present invention has been described in detail above, but the invention be not limited to it is described
Embodiment, those skilled in the art can also make various equivalent on the premise of not violating the inventive spirit of the present invention
Variation or replacement, these equivalent variation or replacement are all included in the scope defined by the claims of the present application.
Claims (7)
1. the flexible nerve electrode that a kind of implanted light stimulus and electrographic recording integrate, which is characterized in that the electrode is by electrode master
Body (1), electric signal interface (2) and fiber stub (3) are constituted.
2. the flexible nerve electrode that a kind of light stimulus according to claim 1 and electrographic recording integrate, which is characterized in that described
Electrode body (1) has guide-lighting substrate (11), hybrid conductive gel (12), electrode microfilament (13), support tungsten wire (14)；Guide-lighting base
Hybrid conductive gel (12), support tungsten wire (14) are wrapped in interior, one section of acicular electrode shape of formation by bottom (11)；The mixing
Signa Gel (12) is filled in the gap between electrode microfilament (13) and guide-lighting substrate (11)；The tail of the electrode microfilament (13)
(131) are held to extend a distance in electrode body (1) rear end.
3. the flexible nerve electrode that a kind of light stimulus according to claim 1 and electrographic recording integrate, which is characterized in that described
The tail end (131) of electrode microfilament (13) and the bottom contact pin (21) of electric signal interface (2) are welded and are connected.
4. the flexible nerve electrode that a kind of light stimulus according to claim 1 and electrographic recording integrate, which is characterized in that described
The outer diameter of electrode body (1) is matched with the internal diameter of fiber stub (3).
5. the flexible nerve electrode that a kind of light stimulus according to claim 2 and electrographic recording integrate, which is characterized in that described
The material of guide-lighting substrate (11) is dimethyl silicone polymer, and the hybrid conductive gel (12) is polyvinyl alcohol and multi-wall carbon nano-tube
The mixed gel of pipe, the electrode microfilament (13) are nichrome or platinumiridio microfilament with insulating layer.
6. the preparation side for the flexible nerve electrode that a kind of implanted light stimulus according to claim 1 and electrographic recording integrate
Method, which is characterized in that carry out in accordance with the following steps:
S1: production electrode leaded light substrate
More tungsten wires are inserted in parallel into a glass capillary, make tungsten wire both ends be exposed on the outside of capillary glass pipe end one section away from
From being affixed on glass pipe outer wall for equally distributed after this distance whole outward bending grown；Silica gel and curing agent are pressed
Mass ratio 10:1 mixing, vacuumizes removal air bubble after mixing evenly, and the poly dimethyl silicon prepared and completed is extracted using syringe
The bottom end for securing the glass capillary of tungsten wire is inserted into syringe needle tube and seals the two gap, slowly pushes injection by oxygen alkane
Device push rod in dimethyl silicone polymer implantation glass pipe, will guarantee that dimethyl silicone polymer is full of pipe internal pore；It is sealed using AB glue
Firmly glass capillary lower end is to prevent dimethyl silicone polymer from flowing out；Glass capillary is disposed vertically into 60 ° of ovens and is solidified,
The bottom end that capillary seal is wiped out after the completion of solidifying stays a tungsten wire to keep the rigidity of electrode, extract out extra tungsten wire formed it is micro-
Channel forms the guide-lighting substrate of electrode；
S2: poly- 3,4- ethene dioxythiophene, polystyrolsulfon acid, multi-walled carbon nanotube electroplate liquid are prepared
Multi-walled carbon nanotube that concentration is 0.2 mg/ml is added in pure water through ultrasonic vibration to mixing well；It is mixing later
Poly- 3,4-rthylene dioxythiophene, the 0.2 wt% polystyrolsulfon acid of 0.1 wt% are successively added in liquid, through ultrasonic vibration until filling
Divide and mixes；
S3: electrode microfilament is prepared
A segment electrode microfilament is taken, is heated by alcolhol burner after removing its microfilament both ends surface insulation layer, one end is welded on electrode
On plug, the other end immerses poly- 3,4-rthylene dioxythiophene, polystyrolsulfon acid, multi-walled carbon nanotube electroplate liquid described in S2
In；Plating modification is carried out by electrochemical workstation three-electrode system, using constant current anodizing process, polymerization electric current is 100nA, plating
Time is 100s, and electrode microfilament is removed after plating；
S4: assembly electrode microfilament
By step described in S3, the preparation of more electrode microfilaments is completed, under microscopic visualization, the electrode microfilament that preparation is completed
In the microchannel for the guide-lighting substrate that insertion S1 completes one by one, guarantee to be inserted into a microfilament in each microchannel, microfilament plating
One end it is facing forward, microchannel front end reserves the gap 5mm, and microfilament extends a segment length in microchannel rear end；
S5: preparing polyvinyl alcohol, multi-walled carbon nanotube Signa Gel for concentration is 0.5 mg/ml multi-walled carbon nanotube and pure water
After mixed liquor ultrasonic vibration mixes well, with the weight proportion of 1:10 be added polyvinyl alcohol and at 80 DEG C heating stirring until filling
Divide dissolution mixing；
S6: perfusion Signa Gel
The made Signa Gel got ready of S5 is extracted with syringe, the guide-lighting substrate front end that electrode microfilament is assembled described in S4 is protruded into
Syringe needle tube simultaneously seals the two gap, slowly pushes injector push-rod, Signa Gel is filled guide-lighting substrate and electrode microfilament
Between gap, unload guide-lighting substrate after the completion and clear up sealing station, tentatively complete electrode body；
S7: freeze thawing is crosslinked Signa Gel
After the S6 electrode body completed is congealed at -20 DEG C, taking-up is melted at room temperature, is so alternately placed repeatedly, until
Signa Gel in electrode body is fully crosslinked；
S8: assembly electrode main body
The electrode body rear end that S7 is completed is inserted into fiber stub together with the more electrode microfilaments to extend out, electrode microfilament is worn
It is welded on electric signal interface after crossing fiber stub, the corresponding signal path of every microfilament；Separately take a naked layer not
The steel wire weld that becomes rusty is used as signal ground on electric signal interface；Electrode body is fixedly bonded on fiber stub using AB glue；With
Glue sealing electrode body both ends；
S9: etching removal electrode outer layer glass capillary
The electrode body leading portion that S8 is assembled is dipped vertically into hydrofluoric acid solution, until outer layer glass capillary is etched completely
It falls off, take out electrode and uses pure water rinsing；
S10: the modification of eletrode tip
Shearing body front end is inclined-plane, makes electrode microfilament about 0.5mm at a distance from inclined-plane, is carried out later by electrochemical workstation
Plating modification, electroplate liquid are poly- 3,4-rthylene dioxythiophene, polystyrolsulfon acid, the multi-walled carbon nanotube plating of preparation described in S2
Liquid, using constant current anodizing process, three-electrode system, polymerization electric current is 100nA, electroplating time 100s；
S11: the encapsulation of electrode
With glue package microfilament and electric signal interface peripheral position, and by electric signal interface and fiber stub fixed bonding, with poly-
The tip of the electrode of completion is electroplated described in ethylene glycol encapsulation S10 and properly places, obtains the flexible nerve electrode.
7. the flexible nerve electrode and preparation method thereof that a kind of light stimulus according to claim 4 and electrographic recording integrate,
It is characterized in that, the diameter range of the electrode body is 200-360 μm, length 1-2cm；The electric signal interface, optical fiber are inserted
Core is standard component.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113047045A (en) * | 2021-03-29 | 2021-06-29 | 中国科学院深圳先进技术研究院 | Flexible optical fiber and preparation method and application thereof |
| CN113197580A (en) * | 2021-04-08 | 2021-08-03 | 中国科学院深圳先进技术研究院 | Method and device for preparing electrode assembly, photoelectrode device and implanting method thereof |
| CN113616212A (en) * | 2021-08-26 | 2021-11-09 | 杭州电子科技大学温州研究院有限公司 | Neural microelectrode array with electrophysiological recording and multi-modal stimulation functions |
| CN113907761A (en) * | 2021-10-09 | 2022-01-11 | 中国人民解放军军事科学院军事医学研究院 | Bipolar implanted myoelectricity electrode assembly for stimulation and recording and application method thereof |
| CN116077829A (en) * | 2023-01-18 | 2023-05-09 | 微智医疗器械有限公司 | Implant device, electro-stimulator, and electrode plating method |
| CN114947868B (en) * | 2021-02-25 | 2024-05-17 | 哈尔滨工业大学 | Flexible neural electrode implantation system and method with force sensing capability |
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| CN114947868B (en) * | 2021-02-25 | 2024-05-17 | 哈尔滨工业大学 | Flexible neural electrode implantation system and method with force sensing capability |
| CN113047045A (en) * | 2021-03-29 | 2021-06-29 | 中国科学院深圳先进技术研究院 | Flexible optical fiber and preparation method and application thereof |
| CN113047045B (en) * | 2021-03-29 | 2022-05-10 | 中国科学院深圳先进技术研究院 | Flexible optical fiber and preparation method and application thereof |
| CN113197580A (en) * | 2021-04-08 | 2021-08-03 | 中国科学院深圳先进技术研究院 | Method and device for preparing electrode assembly, photoelectrode device and implanting method thereof |
| WO2022213655A1 (en) * | 2021-04-08 | 2022-10-13 | 中国科学院深圳先进技术研究院 | Method and device for preparing electrode assembly, photoelectrode device and implantation method therefor |
| CN113197580B (en) * | 2021-04-08 | 2023-10-24 | 中国科学院深圳先进技术研究院 | Electrode assembly preparation method and device, photoelectrode device and implantation method thereof |
| CN113616212A (en) * | 2021-08-26 | 2021-11-09 | 杭州电子科技大学温州研究院有限公司 | Neural microelectrode array with electrophysiological recording and multi-modal stimulation functions |
| CN113907761A (en) * | 2021-10-09 | 2022-01-11 | 中国人民解放军军事科学院军事医学研究院 | Bipolar implanted myoelectricity electrode assembly for stimulation and recording and application method thereof |
| CN113907761B (en) * | 2021-10-09 | 2024-05-07 | 中国人民解放军军事科学院军事医学研究院 | Bipolar implantable myoelectric electrode assembly for stimulation and recording and application method thereof |
| CN116077829A (en) * | 2023-01-18 | 2023-05-09 | 微智医疗器械有限公司 | Implant device, electro-stimulator, and electrode plating method |
| CN116077829B (en) * | 2023-01-18 | 2023-12-15 | 微智医疗器械有限公司 | Implant device, electro-stimulator, and electrode plating method |
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