CN1195222C - Method for producing complex type microelectrode - Google Patents
Method for producing complex type microelectrode Download PDFInfo
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- CN1195222C CN1195222C CNB031374697A CN03137469A CN1195222C CN 1195222 C CN1195222 C CN 1195222C CN B031374697 A CNB031374697 A CN B031374697A CN 03137469 A CN03137469 A CN 03137469A CN 1195222 C CN1195222 C CN 1195222C
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- carbon fiber
- glass capillary
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Abstract
The present invention relates to a method for preparing a composite microelectrode. Silver wires, platinum wires and carbon fibers in a micron level are insulated and sealed in the inner cavity of a thin metal tube by a glass capillary, wherein both ends of the glass capillary of a carbon fiber electrode are melted and sealed by solid paraffin wax; a miniature silver or silver chloride (Ag/AgCl) reference electrode seals a segment of potassium chloride saturated solution of saturated silver chloride in the capillary by the capillary phenomenon of the glass capillary; both ends of the glass capillary of the electrode are sealed by melted solid paraffin wax by the platinum wires. The microelectrode can be used in an environment moving frequently. The present invention particularly relates to a new method for manufacturing a miniature reference electrode in the composite microelectrode. The miniature silver or silver chloride (Ag/AgCl) reference electrode can provide stable reference electric potential in cell measurement.
Description
Affiliated technical field
The present invention relates to the preparation method of the compound microelectrode of a kind of electro-chemical test, research usefulness, it has provided a kind of a kind of new preparation method that stable reference voltage, Trinitarian compound microelectrode can be provided.
Background technology
At present, known silver/silver chloride (Ag/AgCl) contrast electrode is to place silver/silver chloride the glass tube of a band ceramic core to form, owing to the reason on the method, it is conventional that silver/the silver chloride electrode volume is relatively large, be difficult to microminiaturization, and at present cell live body, original position, in the online study, conventional silver/silver chloride electrode is because volume is big, can't satisfy the requirement that stable potential is provided in the cell measurement, and directly adopt silver electrode the unsettled shortcoming of current potential to be arranged as contrast electrode, so that in using conventional electrochemical method such as cyclic voltammetry, can't accurately measure.And three electrodes disperse when measuring, and are not easy to operate.
Summary of the invention
Can not carry out the cell live body in order to overcome existing silver/silver chloride contrast electrode, original position, online electrochemical research, directly do the unstable and existing three electrode dispersion of contrast electrode current potential with filamentary silver, fragile and not easy-operating deficiency, the present invention proposes a kind of method of microminiaturized silver/silver chloride electrode, this method not only volume is little, can insert in the cell, and can provide stable reference potential, and, adopt glass capillary to make insulator and supporter three kinds of microelectrodes, be assembled in the thin metal pipe of a millimeter level internal diameter, be welded as a whole with the miniplug of standard, can provide a kind of simple, easy-to-use and non-damageable compound microelectrode.
The present invention solves the scheme that its technical matters adopts:
1. in having drawn the glass capillary of given shape, the silver that insertion was handled with electrochemical method/silver chloride filament, utilize the capillarity of glass capillary, in kapillary, seal the saturated potassium chloride solution of one section saturated silver chloride up for safekeeping, stable reference potential can be provided.
2. adopt glass capillary as the insulator between three electrodes and support each electrode, adopt the method for secondary copper coating and welding, reduce resistance and background noise that the traditional carbon fibres electrode adopts the conducting resinl sealing-in, improve resolution, and the way of employing and standard plug assembling welding, make it to become one, easily take easy-to-use.
The invention has the beneficial effects as follows that can provide stable on-the-spot reference potential when adopting the electrochemical method three-electrode system to carry out cell measurement, method is simply effective.And, three electrodes of traditional dispersion are carried out microminiaturization, and become one, be easy to use.
Description of drawings
The present invention is further described below in conjunction with accompanying drawing.
Fig. 1: compound microelectrode structural profile synoptic diagram
Among Fig. 1: 1. the miniature working electrode of carbon fiber, 2. silver/silver chloride (Ag/AgCl) miniature reference electrodes, 3. platinum (Pt) is miniature to electrode, 4. thin metal pipe, 5. electrode plug.
Embodiment:
1. the assembling of the miniature working electrode of carbon fiber (1):
Carbon fiber supports with glass capillary insulation and realizes being connected of carbon fiber and copper wire through the secondary copper plating treatment, and the glass capillary two ends are melted with solid paraffin and sealed;
2. the assembling of silver/silver chloride (Ag/AgCl) miniature reference electrodes (3):
In the glass capillary that draws good given shape, the silver that insertion was handled with electrochemical method/silver chloride filament, utilize the capillarity of glass capillary, in kapillary, seal the saturated potassium chloride solution of one section saturated silver chloride up for safekeeping, stable reference potential can be provided, measure the tip carefully to micron order;
3. the miniature assembling of platinum (Pt) to electrode (2):
Thin platinum filament seals with the solid paraffin fusing with glass capillary insulation, support, glass capillary two ends, and it is miniature to electrode (2) to make platinum (Pt);
4. the assembling of compound microelectrode:
Three microelectrodes: in thin metal pipe (4) inner chamber, the contact conductor soldering is in three terminals of electrode plug (5) to electrode (2) mutual encapsulation for carbon fiber working electrode (1), silver/silver chloride miniature reference electrodes (3) and platinum filament.
Claims (4)
1. compound microelectrode, by the miniature working electrode of carbon fiber (1), miniature silver/silver chloride contrast electrode (2) and platinum filament electrode (3) is formed, it is characterized in that: micron-sized filamentary silver, platinum filament and carbon fiber are respectively charged into as after in the glass capillary of insulator and stilt, reinstall in a thin metal pipe (4) inner chamber, three electrode lead-in wire is welded in respectively on the corresponding binding post of electrode plug.
2. compound microelectrode according to claim 1 is characterized in that: miniature silver/silver chloride contrast electrode (2) is a saturated potassium chloride solution of sealing one section saturated silver chloride in the glass capillary after inserting silver/silver chloride filament up for safekeeping;
3. compound microelectrode according to claim 1 is characterized in that: the carbon fiber in the miniature working electrode of carbon fiber (1) is realized being connected of carbon fiber and copper wire through the secondary copper plating treatment.
4. compound microelectrode according to claim 1 is characterized in that: carbon fiber working electrode (1) and platinum filament are housed the glass capillary two ends of electrode (3) are sealed with the solid paraffin fusing;
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031374697A CN1195222C (en) | 2003-06-25 | 2003-06-25 | Method for producing complex type microelectrode |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031374697A CN1195222C (en) | 2003-06-25 | 2003-06-25 | Method for producing complex type microelectrode |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1462882A CN1462882A (en) | 2003-12-24 |
| CN1195222C true CN1195222C (en) | 2005-03-30 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB031374697A Expired - Fee Related CN1195222C (en) | 2003-06-25 | 2003-06-25 | Method for producing complex type microelectrode |
Country Status (1)
| Country | Link |
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| CN (1) | CN1195222C (en) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100342049C (en) * | 2005-09-09 | 2007-10-10 | 浙江大学 | Cubic nanometer ZnMgO wire material and its prepn |
| CN100392388C (en) * | 2005-09-23 | 2008-06-04 | 中国科学院长春应用化学研究所 | Preparation method of electrochemical combined electrode |
| CN100386622C (en) * | 2006-03-20 | 2008-05-07 | 西安交通大学 | Super micro conic electrode and its preparing method |
| CN100431487C (en) * | 2007-01-25 | 2008-11-12 | 中国科学院上海微系统与信息技术研究所 | Processing method of three-dimensional implantable microelectrode array |
| CN101059477B (en) * | 2007-05-11 | 2013-10-16 | 浙江大学 | Cell growth and configuration change checking device based on oxidation/reduction probe, and the checking method |
| CN101408526B (en) * | 2008-11-14 | 2012-07-04 | 西安建筑科技大学 | Dissolved oxygen micro electrode and preparing method thereof |
| CN101430322B (en) * | 2008-12-12 | 2012-07-18 | 国家纳米技术与工程研究院 | Operation method for non-destructive estimation of nano-dimension glass microprobe performance |
| CN102359985B (en) * | 2011-08-01 | 2013-11-13 | 南京大学 | Coaxial microelectrode for microfluidic chip and preparation method thereof |
| CN105203607B (en) * | 2015-10-29 | 2018-11-20 | 东南大学 | A kind of preparation method of carbon fibre ultramicro-electrode |
| CN108490052A (en) * | 2018-02-08 | 2018-09-04 | 重庆文理学院 | A kind of novel carbon fiber microelectrode and preparation method thereof |
| CN111505071B (en) * | 2020-04-13 | 2022-06-10 | 商丘师范学院 | Integrated microelectrode sensor for simultaneous detection of pH and AA and preparation method and application thereof |
| CN112345616B (en) * | 2020-09-14 | 2022-03-11 | 中国科学院生物物理研究所 | High-precision electrochemical detection method suitable for detecting biological active substances at single cell and sub-cell level |
| CN114001797A (en) * | 2021-10-25 | 2022-02-01 | 中航通飞华南飞机工业有限公司 | Water tank water amount measuring system and method based on large fire-fighting aircraft |
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2003
- 2003-06-25 CN CNB031374697A patent/CN1195222C/en not_active Expired - Fee Related
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| Publication number | Publication date |
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| CN1462882A (en) | 2003-12-24 |
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