CN109221895A - Feeding biodegradable mycotoxin preparation of one kind and its preparation method and application - Google Patents
Feeding biodegradable mycotoxin preparation of one kind and its preparation method and application Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/25—Removal of unwanted matter, e.g. deodorisation or detoxification using enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K20/189—Enzymes
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/27—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/28—Removal of unwanted matter, e.g. deodorisation or detoxification using microorganisms
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Abstract
The present invention provides a kind of feeding biodegradable mycotoxin preparation, it is made of fungus solids fermentation material, yeast cell wall polysaccharide and complex enzyme formulation, the weight ratio of the fungus solids fermentation material, yeast cell wall polysaccharide and complex enzyme formulation is (2-6): (1-3): (1-3), the fungus solids fermentation material is that compound bacteria is isolated from Divine Comedy, and compound bacteria is mixed into solid medium and is fermented.Feeding biodegrade mycotoxin preparation prepared by the present invention all has the multiple actions such as inhibition generation, degradation and absorption to common mycotoxins a variety of in feed, it can be effectively controlled and solve pollution of the mycotoxin to grain and feed, have very important meaning safely to improving breeding performonce fo animals and improving human food.
Description
Technical field
The present invention relates to animal feed additive fields, and in particular to a kind of feeding biodegradable mycotoxin preparation and its
Preparation method and application.
Background technique
Mycotoxin is produced by the mycetogenetic toxic secondary metabolism of the different types such as Aspergillus, Penicillium notatum and sickle-like bacteria
Object, current main mycotoxin includes aflatoxin (Aflatoxin, AFT), Trichothecenes toxin
(Tirchothecenes mainly has HT-2 toxin, NEO and DON), zearalenone (Zearalenone, ZEN), reddish brown song
Mould toxin (Ochratoxin A, OTA), fumarubicin (Fumonisins) and vomitoxin etc..Wherein recall rate in feed
It is relatively high and bigger mycotoxin is influenced mainly on animal have aflatoxin, zearalenone and vomitoxin.It is dynamic
Daily ration of the object feeding containing aflatoxin, can not only reduce animal feed conversion efficiency, reduce meat yield, egg production and milk production
Amount increases animal disease and the death rate, also causes huge economic loss to animal husbandry, can also remain in animal product,
It causes people to be poisoned, causes stomach, lesions of liver and kidney, or even can mutagenesis, carcinogenic and immunosupress etc..Therefore, effectively control reconciliation
Certainly pollution of the mycotoxin to grain and feed has safely improvement breeding performonce fo animals and raising human food very important
Meaning.
The method for studying more degradation mycotoxin at present has chemical method, physical method and biological degradation method.But it is chemical
Greatest problem existing for method is that chemical substance has corrosivity, can destroy the nutritional ingredient of feed, reduce feed palatability.This
Outside, chemical substance also has certain harm to animal body health, and therefore, operability is not strong in production for chemical method.
While physical absorbent absorbing toxin also can nutriment in adsorption feed, to cause economic loss.Mycotoxin
Biological degradation method, which refers to that the enzyme that the toxophore of mycotoxin is generated by microorganism or secondary metabolite decompose, to be destroyed, and is generated
The process of nontoxic product.Biological degradation method have removing toxic substances thoroughly, specificity it is strong, it is pollution-free on feed, do not influence feed nutrition
It is worth, avoids the advantages that regenerating of toxin, thus by the concern of researcher.
Divine Comedy is widely used one kind in traditional zymotic class Chinese medicine on Chinese history, while being also in history earliest one
Kind fungus solids fermented product.Wherein main bacteria seed has light white latent ball yeast (Cryptococcus albidus), saccharomyces cerevisiae
In (Saccharomyces cerevisiae), pichia kudriavzevii (Pichiakudriavzevii), button capsule are quasi-
Spore yeast (Endomycesfibuliger) etc., aspergillus flavus (Aspergillusflavus), Rhizopus oryzae
(Rhizopusoryzae), aspergillus niger (A. niger), A. ambiguus, A. ivoriensis, absidia corymbifera
(Absidiacorymbifera) etc., bacterium has Klebsiella (Klebsiella sp.), Enterobacter
(Enterobacter), pseudomonad (Pseudomonas), acinetobacter calcoaceticus (Acinetobacter) and Bacillus acidi lactici etc..And incite somebody to action
Complicated microorganism in Divine Comedy is separated, and is applied in solid fermentation, using the various enzymes of its secretion come in feed of degrading
Mycotoxin also needs further research and development.
Summary of the invention
To solve the above problems, the present invention provides feeding biodegradable mycotoxin preparation of one kind and preparation method thereof and answers
With.
To achieve the above object, the present invention adopts the following technical scheme: a kind of feeding biodegradable mycotoxin preparation, by
Fungus solids fermentation material, yeast cell wall polysaccharide and complex enzyme formulation composition, the fungus solids fermentation material, yeast cell wall are more
The weight ratio of sugar and complex enzyme formulation is (2-6): (1-3): (1-3).
Preferably, the weight ratio of the fungus solids fermentation material, yeast cell wall polysaccharide and complex enzyme formulation is 2:1:
1。
Preferably, the fungus solids fermentation material is to isolate compound bacteria from Divine Comedy, compound bacteria is mixed into solid training
It supports and ferments in base.
Preferably, the complex enzyme formulation includes carboxypeptidase, lipase, neutral proteinase, lactone hydrolase, recombination Huang
The content of aspertoxin detoxication enzyme, various enzyme preparations is equal.
A method of preparing above-mentioned feeding biodegradable mycotoxin preparation, comprising the following steps: (1) compound bacteria liquid
Preparation: Divine Comedy is smashed it through into 60 meshes, smashed Medicated Leaven is divided into the nutrient meat that two equal portions are separately added into 200mL
In the potato culture of soup culture medium and 200mL, for 24 hours, the two is uniformly mixed sterile culture, in sterile item under the conditions of 35 DEG C
Culture solution is filtered in sterile beaker with sterile gauze under part, remaining solid object is repeated the above steps twice, finally by institute
Liquid mixing must be filtered to shake up, it is spare to be placed in preservation in 4 DEG C of low temperature refrigerators；(2) preparation of solid medium: by dregs of beans, wheat bran,
Cotton dregs, rde bean, corn flour, corn pulp, molasses, nutritive salt pour into conical flask after mixing, and addition distilled water reaches water content
To 60%, in 121 DEG C of sterilizing 20min after mixing, after cooling, solid medium is made；(3) preparation of fungus solids fermentation material:
Step (1) resulting compound bacteria liquid is added in solid medium by the additive amount of solid medium weight 10%, after mixing
35 DEG C are put in, 48h is cultivated, resulting solid culture natural air drying crushes, crosses 40-60 mesh, then uses pasteurization
Method carries out sterilizing, and fungus solids fermentation material is made；(4) it the preparation of biodegradable mycotoxin preparation: prepared by step (3)
Fungus solids fermentation material mixed with yeast cell wall polysaccharide and complex enzyme formulation, stir evenly to get biodegradable mould poison
Plain preparation.
Preferably, the solid medium by mass fraction be 20% dregs of beans, 40% wheat bran, 10% cotton dregs, 10% rde bean,
10% corn flour, 5% corn pulp, 3% molasses, 2% nutritive salt composition.
Preferably, the nutritive salt is NH4NO3、KH2PO4、Ca SO4、Mg SO4In any or combinations thereof object.
Application of the feeding biodegrade mycotoxin preparation in the cultivation of beast fowl, by the feeding biodegradable mycotoxin
Preparation is added in animal diets, including pig with, fowl with and ruminant daily ration.
The beneficial effects of the present invention are: feeding biodegradable mycotoxin preparation is to more in feed prepared by the present invention
The common mycotoxin of kind all has the multiple actions such as inhibition generation, degradation and absorption, can be effectively controlled and solve mycotoxin pair
The pollution of grain and feed has safely very important meaning to improving breeding performonce fo animals and improving human food；The present invention
Complicated microorganism in Divine Comedy is separated, is applied in solid fermentation, using the various enzymes of its secretion come in feed of degrading
Mycotoxin, can for degradation mycotoxins in feed research and development on some helps and thinking are provided.
Specific embodiment
The present invention is further explained in the light of specific embodiments, but is not limited to following embodiment.
A kind of feeding biodegradable mycotoxin preparation, by fungus solids fermentation material, yeast cell wall polysaccharide and complex enzyme
The weight ratio of preparation composition, the fungus solids fermentation material, yeast cell wall polysaccharide and complex enzyme formulation is (2-6): (1-3):
(1-3).
Fungus solids fermentation material is that compound bacteria is isolated from Divine Comedy, compound bacteria is mixed into solid medium ferment and
At.
Complex enzyme formulation includes carboxypeptidase, lipase, neutral proteinase, lactone hydrolase, the removing toxic substances of recombined Aspergillus flavus toxin
The content of enzyme, various enzyme preparations is equal.
The preparation method of feeding biodegrade mycotoxin preparation the following steps are included: (1) compound bacteria liquid preparation: take
30-60g Divine Comedy smashes it through 40-60 mesh, and smashed Medicated Leaven is divided into the nutrient meat that two equal portions are separately added into 200mL
In the potato culture of soup culture medium and 200mL, sterile culture 18-30 h, the two is uniformly mixed under the conditions of 35 DEG C,
Culture solution is filtered in sterile beaker with sterile gauze under aseptic condition, remaining solid object repeats the above steps twice, finally
Gained filtering liquid is mixed and is shaken up, it is spare to be placed in preservation in 4 DEG C of low temperature refrigerators；(2) preparation of solid medium: by dregs of beans,
Wheat bran, cotton dregs, rde bean, corn flour, corn pulp, molasses, nutritive salt pour into conical flask after mixing, and addition distilled water makes aqueous
Amount reaches 50-70%, and in 121 DEG C of sterilizing 15-30 min after mixing, after cooling, solid medium is made；(3) fungus solids are sent out
The preparation of ferment object: step (1) resulting compound bacteria liquid is added to solid culture by the additive amount of solid medium weight 10%
It is put in 35 DEG C in base, after mixing, cultivates 36-60 h, resulting solid culture natural air drying crushes, crosses 40-60 mesh,
Then sterilizing is carried out using pasteurization, fungus solids fermentation material is made；(4) system of biodegradable mycotoxin preparation
It is standby: fungus solids fermentation material prepared by step (3) being mixed with yeast cell wall polysaccharide and complex enzyme formulation, is stirred evenly, i.e.,
Obtain biodegradable mycotoxin preparation.
Solid medium is 20% dregs of beans, 40% wheat bran, 10% cotton dregs, 10% rde bean, 10% corn flour, 5% by mass fraction
Corn pulp, 3% molasses, 2% nutritive salt composition.
Nutritive salt is NH4NO3、KH2PO4、Ca SO4、Mg SO4In any or combinations thereof object.
Feeding biodegradable mycotoxin preparation prepared by the present invention is added in animal diets, including pig is used, fowl to
And ruminant daily ration.
Embodiment 1
A kind of feeding biodegradable mycotoxin preparation, preparation method the following steps are included: (1) compound bacteria liquid preparation: take
40g Divine Comedy smashes it through 60 meshes, and smashed Medicated Leaven is divided into two equal portions and is separately added into 200ml nutrient broth medium
In 200mL potato culture, under the conditions of 35 DEG C sterile culture for 24 hours after, the two mixing shake up, aseptically use nothing
Bacterium gauze filters culture solution in sterile beaker, remaining solid object is repeated the above steps twice, finally by gained filtered fluid
Body mixing shakes up, and it is spare to be placed in preservation in 4 DEG C of low temperature refrigerators；(2) preparation of solid medium: by mass fraction be 20% dregs of beans,
40% wheat bran, 10% cotton dregs, 10% rde bean, 10% corn flour, 5% corn pulp, 3% molasses, 2% NH4NO3Conical flask is poured into after mixing
In, addition distilled water makes water content reach 60%, and in 121 DEG C of 20 min of sterilizing after mixing, after cooling, solid medium is made；
(3) step (1) resulting compound bacteria liquid the preparation of fungus solids fermentation material: is pressed to the additive amount of solid medium weight 10%
It is added in solid medium, 35 DEG C is put in after mixing, cultivate 48 h, resulting solid culture natural air drying crushes, mistake
Then 60 meshes carry out sterilizing using pasteurization, to retain part microorganism and protease activity, fungus solids are made
Fermentation material；(4) preparation of biodegradable mycotoxin preparation: fungus solids fermentation material and yeast cells prepared by step (3)
Wall polysaccharide and complex enzyme formulation carry out compounding mixing according to 2:1:1, stir evenly to get biodegradable mycotoxin preparation,
Middle complex enzyme formulation is carboxypeptidase, the lipase, neutral proteinase, lactone hydrolase, recombined Aspergillus flavus toxin solution of impartial mixing
Toxenzyme.
Embodiment 2
A kind of feeding biodegradable mycotoxin preparation, preparation method the following steps are included: (1) compound bacteria liquid preparation: take
30g Divine Comedy smashes it through 40 meshes, and smashed Medicated Leaven is divided into two equal portions and is separately added into 200ml nutrient broth medium
In 200mL potato culture, under the conditions of 35 DEG C after sterile culture 18h, the two mixing is shaken up, and aseptically uses nothing
Bacterium gauze filters culture solution in sterile beaker, remaining solid object is repeated the above steps twice, finally by gained filtered fluid
Body mixing shakes up, and it is spare to be placed in preservation in 4 DEG C of low temperature refrigerators；(2) preparation of solid medium: by mass fraction be 20% dregs of beans,
40% wheat bran, 10% cotton dregs, 10% rde bean, 10% corn flour, 5% corn pulp, 3% molasses, 2% MgSO4Conical flask is poured into after mixing
In, addition distilled water makes water content reach 50%, and in 121 DEG C of 15 min of sterilizing after mixing, after cooling, solid medium is made；
(3) step (1) resulting compound bacteria liquid the preparation of fungus solids fermentation material: is pressed to the additive amount of solid medium weight 10%
It is added in solid medium, 35 DEG C is put in after mixing, cultivate 36 h, resulting solid culture natural air drying crushes, mistake
Then 40 meshes carry out sterilizing using pasteurization, to retain part microorganism and protease activity, fungus solids are made
Fermentation material；(4) preparation of biodegradable mycotoxin preparation: fungus solids fermentation material and yeast cells prepared by step (3)
Wall polysaccharide and complex enzyme formulation carry out compounding mixing according to 5:3:2, stir evenly to get biodegradable mycotoxin preparation,
Middle complex enzyme formulation is carboxypeptidase, the lipase, neutral proteinase, lactone hydrolase, recombined Aspergillus flavus toxin solution of impartial mixing
Toxenzyme.
Embodiment 3
A kind of feeding biodegradable mycotoxin preparation, preparation method the following steps are included: (1) compound bacteria liquid preparation: take
60g Divine Comedy smashes it through 50 meshes, and smashed Medicated Leaven is divided into two equal portions and is separately added into 200ml nutrient broth medium
In 200mL potato culture, under the conditions of 35 DEG C after sterile culture 30h, the two mixing is shaken up, and aseptically uses nothing
Bacterium gauze filters culture solution in sterile beaker, remaining solid object is repeated the above steps twice, finally by gained filtered fluid
Body mixing shakes up, and it is spare to be placed in preservation in 4 DEG C of low temperature refrigerators；(2) preparation of solid medium: by mass fraction be 20% dregs of beans,
40% wheat bran, 10% cotton dregs, 10% rde bean, 10% corn flour, 5% corn pulp, 3% molasses, 2% KH2PO4With Ca SO4Mixture is mixed
It being poured into conical flask after even, addition distilled water makes water content reach 70%, in 121 DEG C of 30 min of sterilizing after mixing, after cooling,
Solid medium is made；(3) step (1) resulting compound bacteria liquid the preparation of fungus solids fermentation material: is pressed into solid medium
The additive amount of weight 10% is added in solid medium, and 35 DEG C are put in after mixing, 60 h is cultivated, by resulting solid culture
Natural air drying crushes, and crosses 60 meshes, then sterilizing is carried out using pasteurization, to retain part microorganism and albumen
Fungus solids fermentation material is made in enzyme activity；(4) preparation of biodegradable mycotoxin preparation: fungus solids prepared by step (3)
Fermentation material carries out compounding mixing according to 4:2:1 with yeast cell wall polysaccharide and complex enzyme formulation, stirs evenly to get biodegrade
Mycotoxin preparation, wherein complex enzyme formulation is the carboxypeptidase of impartial mixing, lipase, neutral proteinase, lactone hydrolase, again
Group Aflatoxin-detofizyme.
Embodiment 4
A kind of feeding biodegradable mycotoxin preparation, preparation method the following steps are included: (1) compound bacteria liquid preparation: take
50g Divine Comedy smashes it through 30 meshes, and smashed Medicated Leaven is divided into two equal portions and is separately added into 200ml nutrient broth medium
In 200mL potato culture, under the conditions of 35 DEG C after sterile culture 27h, the two mixing is shaken up, and aseptically uses nothing
Bacterium gauze filters culture solution in sterile beaker, remaining solid object is repeated the above steps twice, finally by gained filtered fluid
Body mixing shakes up, and it is spare to be placed in preservation in 4 DEG C of low temperature refrigerators；(2) preparation of solid medium: by mass fraction be 20% dregs of beans,
40% wheat bran, 10% cotton dregs, 10% rde bean, 10% corn flour, 5% corn pulp, 3% molasses, 2% NH4NO4And MgSO4Mixture mixes
After pour into conical flask, addition distilled water so that water content is reached 55%, in 121 DEG C of 25 min of sterilizing after mixing, after cooling, make
Obtain solid medium；(3) step (1) resulting compound bacteria liquid the preparation of fungus solids fermentation material: is pressed into solid culture base weight
The additive amount of amount 10% is added in solid medium, and 35 DEG C are put in after mixing, cultivates 50 h, certainly by resulting solid culture
It so air-dries, crushes, cross 50 meshes, sterilizing is then carried out using pasteurization, to retain part microorganism and protease
It is living, fungus solids fermentation material is made；(4) preparation of biodegradable mycotoxin preparation: the fungus solids of step (3) preparation are sent out
Ferment object carries out compounding mixing according to 6:2:3 with yeast cell wall polysaccharide and complex enzyme formulation, stirs evenly mould to get biodegrade
Verticillium toxin preparation, wherein complex enzyme formulation is carboxypeptidase, the lipase, neutral proteinase, lactone hydrolase, recombination of impartial mixing
Aflatoxin-detofizyme.
Embodiment 5
One, degradation mycotoxin imitates the directly degradation of vomitoxin DON, aflatoxin AFB1 and zearalenone ZEN
Fruit detection.
(1) preparation of vomitoxin DON
Vomitoxin DON powder is dissolved in ultrapure water, 1mg/mL is configured to, liquid-transfering gun draws 1mL, is diluted to 50 μ g/mL
The mother liquor of concentration is filtered with syringe needle filter and is dispensed, is stored in 4 DEG C.
(2) preparation of aflatoxins AFB1 solution
Aflatoxins AFB1 is dissolved in CH2Cl2In, 100 μ g/ml are finally obtained as mother liquor, are filtered and are dispensed with syringe needle filter, protected
It is stored in 4 DEG C.
(3) preparation of zearalenone ZEN solution
Zearalenone ZEN is dissolved in CH2Cl2In, 100 μ g/ml are finally obtained as mother liquor, with syringe needle filter filtering point
Dress, is stored in 4 DEG C.
(4) detection toxin degradation
The preparation prepared in 10g embodiment 1 is taken, is placed in 100mL distilled water, shaking flask shakes 12h, then in 8000rpm/min,
Be centrifuged 20min under the conditions of 4 DEG C, take supernatant 10mL be placed in 4 DEG C it is spare.Then respectively that configured DON, AFB1, ZEN is molten
Liquid is added in spare supernatant, is made into corresponding detectable concentration respectively, is put under the conditions of 37 DEG C after shaking up, and avoid light place is for 24 hours
It is spare afterwards.
(5) 3 kinds of content of toxins are detected using direct competive ELISA detection method
The experimental results showed that invention formulation to DON, AFB1 degradation rate up to 80% or so, to ZEN degradation rate up to 85%.
Two, to the measurement of tri- kinds of content of toxins of DON, AFB1, ZEN in moldy feed.
(1) sample treatment
It takes the preparation prepared in 100g embodiment 1 to be mixed with 500g feed, places after mixing spare for 24 hours.
(2) content of toxins detects
Three kinds of content of toxins are measured using liquid chromatography according to National Standard Method.
The experimental results showed that invention formulation has significant decrease effect to AFB1, ZEN content, there is reduction to imitate DON content
Fruit, but difference is not significant.
Embodiment 6
Influence of the biological agent to Production Performance of Weaning Pigs
It selects the ternary hybrid weanling pig that health similar in 60 35 age in days weight is grown up and is randomly divided into 3 groups, blank control group
Basic drawing is fed, positive controls add 300 mg/t of aflatoxin B1 in Basic drawing, and test group is in positive control
1% degrading mold toxin agent is added in group diet, using average daily gain, average daily gain, feed-weight ratio, diarrhea rate as index, is ground
Study carefully influence of the degrading mold toxin agent to Growth Performance of Weaning Piglets.
One each group weanling pig growing state of table compares
The experimental results showed that 1% degrading mold toxin agent, compared with aflatoxin positive controls, average daily gain is averaged
Daily gain is significantly increased (P < 0.05), and feed-weight ratio, diarrhea rate significantly reduce (P < 0.05), and compared with blank control group
Difference is not significant (P > 0.05).
Embodiment 7
Influence of the biological agent to Broiler chicks production performance and immune function
It chooses 90 plumage, 1 age in days AA Broiler chicks and is randomly divided into 3 groups, blank control group feeds Basic drawing, and positive controls are on basis
200 mg/t of aflatoxin B1 is added in diet, test group adds 1% degrading mold toxin agent in positive controls diet,
Using average daily gain, average daily gain, feed-weight ratio, immune organ, serum biochemistry as index, degrading mold toxin agent pair is studied
The influence of broiler growth performance and immune function.
Two each group broiler growth situation of table compares
Three each group broiler growth situation of table compares
Four each group Broiler chicks immune function of table compares
The experimental results showed that 1% degrading mold toxin agent, compared with aflatoxin positive controls, average daily gain is averaged
Daily gain is significantly increased (P < 0.05), and feed-weight ratio significantly reduces (P < 0.05), and difference is not compared with blank control group
Significantly (P > 0.05).Test group thymus index, index and spleen index, bursal index significantly improve (P < relative to positive controls
0.05) difference is not significant (P > 0.05), and compared with blank control group.Test group urea nitrogen content is significant compared with positive controls
Reduce (P < 0.05), total protein, albumin content significantly increase (P < 0.05), and total cholesterol, content of triglyceride without
Significant changes (P > 0.05).
The above is the preferred embodiment of the present invention, it is noted that for those skilled in the art, the present invention
It is not restricted to the described embodiments, under the premise of not departing from general idea of the present invention, several changes and improvements can also be made,
These also should be considered as protection scope of the present invention.
Claims (8)
1. a kind of feeding biodegradable mycotoxin preparation, it is characterised in that: by fungus solids fermentation material, yeast cell wall polysaccharide
It is formed with complex enzyme formulation, the weight ratio of the fungus solids fermentation material, yeast cell wall polysaccharide and complex enzyme formulation is (2-
6): (1-3): (1-3).
2. feeding biodegradable mycotoxin preparation as described in claim 1, it is characterised in that: the fungus solids fermentation
The weight ratio of object, yeast cell wall polysaccharide and complex enzyme formulation is 2:1:1.
3. feeding biodegradable mycotoxin preparation as described in claim 1, it is characterised in that: the fungus solids fermentation material
It is to isolate compound bacteria from Divine Comedy, compound bacteria is mixed into solid medium and is fermented.
4. feeding biodegradable mycotoxin preparation as described in claim 1, it is characterised in that: the complex enzyme formulation includes
Carboxypeptidase, lipase, neutral proteinase, lactone hydrolase, recombined Aspergillus flavus toxin detoxication enzyme, the content phase of various enzyme preparations
Deng.
5. a kind of method for preparing feeding biodegradable mycotoxin preparation as described in claim 1, it is characterised in that: including
Following steps: (1) preparation of compound bacteria liquid: taking 30-60g Divine Comedy to smash it through 40-60 mesh, by smashed Medicated Leaven
It is divided into two equal portions to be separately added into the nutrient broth medium of 200mL and the potato culture of 200mL, the nothing under the conditions of 35 DEG C
Bacterium cultivates 18-30 h, and the two is uniformly mixed, and is aseptically filtered culture solution in sterile beaker with sterile gauze,
Remaining solid object repeats the above steps twice, finally mixes gained filtering liquid and shakes up, it is standby to be placed in preservation in 4 DEG C of low temperature refrigerators
With；(2) preparation of solid medium: dregs of beans, wheat bran, cotton dregs, rde bean, corn flour, corn pulp, molasses, nutritive salt are mixed
After pour into conical flask, addition distilled water so that water content is reached 50-70%, in 121 DEG C of sterilizing 15-30 min after mixing, wait cool
Afterwards, solid medium is made；(3) step (1) resulting compound bacteria liquid the preparation of fungus solids fermentation material: is pressed into solid culture
The additive amount of base weight amount 10% is added in solid medium, and 35 DEG C are put in after mixing, cultivates 36-60 h, resulting solid is trained
Object natural air drying is supported, is crushed, 40-60 mesh is crossed, sterilizing is then carried out using pasteurization, fungus solids fermentation is made
Object；(4) preparation of biodegradable mycotoxin preparation: the fungus solids fermentation material of step (3) preparation and yeast cell wall is more
Sugar and complex enzyme formulation mixing, stir evenly to get biodegradable mycotoxin preparation.
6. the preparation method of feeding biodegradable mycotoxin preparation as claimed in claim 5, it is characterised in that: the solid
Culture medium is 20% dregs of beans, 40% wheat bran, 10% cotton dregs, 10% rde bean, 10% corn flour, 5% corn pulp, 3% sugar by mass fraction
Honey, 2% nutritive salt composition.
7. the preparation method of feeding biodegradable mycotoxin preparation as claimed in claim 6, it is characterised in that: the nutrition
Salt is NH4NO3、KH2PO4、Ca SO4、Mg SO4In any or combinations thereof object.
8. a kind of application of feeding biodegradable mycotoxin preparation as described in claim 1 in the cultivation of beast fowl, will be described
Feeding biodegrade mycotoxin preparation is added in animal diets, including pig with, fowl with and ruminant daily ration.
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