CN109220778A - The breeding method of radish - Google Patents
The breeding method of radish Download PDFInfo
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- CN109220778A CN109220778A CN201811147435.3A CN201811147435A CN109220778A CN 109220778 A CN109220778 A CN 109220778A CN 201811147435 A CN201811147435 A CN 201811147435A CN 109220778 A CN109220778 A CN 109220778A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
- A01H1/08—Methods for producing changes in chromosome number
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Abstract
The present invention relates to crop breeding fields, the in particular to breeding method of radish.The present invention formulates a series of evening a kind of sedge radish DH systems using radish haploid breeding technology；The measurement of male sterility retentivity, then using maintenance DH system as recurrent parent, transformation DH male sterile line are carried out to the DH system of acquisition；A collection of candidate material is picked out from the above-mentioned initiative material obtained by breeding objective, and is female parent with DH male sterile line, and DH system is male parent, is combined power measurement, filters out advantage cross combination.The situation of spring white radishes seed heavy dependence import has been effectively relieved in breeding method provided by the invention.The successful breeding and commercial applications of middle trailing plants No.1 as domestic spring white radishes replacement kind, further enrich the kind deposit of domestic spring white radishes, the whole synthesis competitiveness of domestic import spring white radishes substitute species is enhanced, spring white radishes seed import substitution process is completed for domestic radish breeding team and enhances technical strength.
Description
Technical field
The present invention relates to crop breeding fields, the in particular to breeding method of radish.
Background technique
Radish is the second largest vegetable crop in China, 18,000,000 mu of sown area of year, area and the China yield Jun Zhan vegetables total amount
10% or so.Radish originates in China, and the radish germ plasm resource of China various regions is very rich, before the miscellaneous excellent seed of radish is planted extensively,
It is at least planted extensively there are four types of above local varieties in each county.But in terms of late bolting germ plasm resource, China extremely lacks,
So that precocious type evening a kind of sedge radish one introduces at the beginning of centralized production, the production model in the transportation and sales whole nation start, it is formed and sweeps across entirely
The agitation of state, and height monopolizes China's Seed Market in the period of recent two decades.
Under the situation of spring white radishes seed heavy dependence import, domestic more than 30 a radish breeding units feel more pressure, but state
Interior original germ plasm resource not can solve problem, can only be hybridized with the first-filial generation introduced with domestic original excellent autumn and winter radish
The radish parent material that we have by oneself is formulated afterwards.
Since import kind is also being kept updating, regular pedigree breeding is difficult the step regenerated with upper inlet Cultivar replacing
It cuts down.Therefore it provides the breeding method of new varieties and new varieties that spring white radishes seed heavy dependence import situation is effectively relieved has
Important realistic meaning.
It is newly sheerly currently, carrying out breeding radish in the method that radish breeding field mainly uses strain to screen.Strain screening method is
The complementary type breeding material for meeting breeding objective is subjected to artificial stripping flower bud emasculation hybridization, obtains F1 generation, that is, first-filial generation, then to this
F1 generation carry out mostly for individual plant selfing, and to the single plant based material in per generation carry out economic characters comparison, filter out excellent single plant into
The follow-on individual plant selfing of row is newly sheerly to select the radish for substantially conforming to require.Although strain screening method can select
The preferable radish routine new varieties of integral economic trait and cross combination, but there are still following defects for this method: first, due to radish
Chromosome number be 9 pairs, not considering to exchange and in the case where transposition recombination factor, every chromosome can be regarded as to a something lost
Hormogon is passed, the homozygous rate of self progeny is only 2-9 i.e. 1/512, and the overwhelming majority is miscellaneous in its any one group of hill in this way
Zoarium, and homozygote is very rare, and is difficult to discriminate between homozygote and heterozygote, to be added significantly to the difficulty of radish varieties breeding
Degree；Second, the most of Heterozygosities of the excellent single plant of variable rate technology type are high, and most of pure in the Breeding Process of radish varieties
The high single plant variable rate technology type of conjunction degree is poor, and the high plant of degree homozygous in this way is easy to be eliminated instead, leads to merit
It loses, it is difficult to obtain the pure line cultivar of optimal set mould assembly；Third, the inbreeding depression phenomenon of radish filial generation is extremely serious, make it
The process for being bred as pure line cultivar is very long, and breeding work efficiency is very low.
Summary of the invention
In view of this, the present invention provides a kind of breeding method of radish.The radish haploid breeding technology of offer is being commercialized
Using upper success, the situation of spring white radishes seed heavy dependence import has been effectively relieved.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides a kind of breeding methods, include the following steps:
Step 1: being maternal, " white jade spring radish " for male parent with " plum blossom spring radish ", DH system is obtained by haploid breeding；
Step 2: the excellent DH system of selection Agronomic characteristic overall merit measures male sterility retentivity, obtains maintenance
DH system obtains DH male sterile line using maintenance DH system as recurrent parent；
Step 3: being maternal, " day letter spring radish " for male parent with " Guangdong spring not old radish ", DH is obtained by haploid breeding
System；The DH pure lines for selecting Agronomic characteristic overall merit excellent are as male parent, the DH male sterile line obtained with step 2
As female parent, diallel cross is carried out, combined power measurement obtains advantage cross combination.
Specifically, breeding method provided by the invention are as follows:
(1) " plum blossom spring radish " is female parent, " white jade spring radish " is male parent, prepares complementary combinations, utilizes radish haploid breeding
Technology formulates 928, DH system, then obtains 9 excellent DH systems through field character overall merit, carries out male to this 9 excellent DH systems
Sterile retentivity measurement, obtains 4, maintenance DH system (ED0108, ED0126, ED0152, ED0198), then is protected with this four DH
Hold is (to refer to the parent in backcrossing for recurrent parent.Backcrossing refer to hybrid that parents generate again with one of parent into
Row hybridization.Generally when hybridizing first time, that there is the kind of good characteristic to make is maternal for choosing, and afterwards each backcrossing when make male parent,
This parent backcrossing when be recurrent parent) obtain DH male sterile line four (ED0108A, ED0126A, ED0152A,
ED0198A)。
(2) it is maternal, " day letter spring radish " for male parent with " Guangdong spring not old radish ", prepares complementary combinations, utilize single times of radish
Body breeding technique formulates 836, DH system, then through field character overall merit obtain 10 excellent DH systems (ED5168, ED5236,
ED5238, ED5265, ED5279, ED5398, ED5628, ED5789, ED5796, ED5863), use these DH systems as male parent material
Material.
(3) take above-mentioned two coordinate force stronger evening bolting male sterile line ED0108A, ED0198A to make maternal, with above-mentioned
The acquisition of acquisition 10 parts of DH pure lines ED5168, ED5236, ED5238, ED5265, ED5279, ED5398, ED5628,
ED5789, ED5796, ED5863 make male parent system, and carrying out diallel cross, (diallel cross design includes total fertilization failure and endless
All diallel crossing.Total fertilization failure carries out all possible hybridization between referring to one group of parent)
To the combination of above-mentioned 20 trials carry out take turns variety comparative tests, the optimum combination filtered out be ED0108A ×
ED5168。
(5) ED0108A × ED5168 has carried out Regional suitability test in multiple radish main producing regions, be significantly better than it is existing into
Mouth main breed, registers through crops validation board of Hunan Province, names as " middle trailing plants No.1 ".
In some specific embodiments of the invention, the haploid breeding specifically: be inoculated in after obtaining explant
Induced medium, until culture to monoploid embryoid occurs；The monoploid embryoid is transferred to after optical culture is afforested
It sprouts culture medium, culture obtains the monoploid regrowth for differentiating bud eye, and the monoploid regrowth is transferred to root media
It takes root, then moves into field production, its blade microscopy is taken when growing 3~5 true leaves, determines its ploidy, to not occurring
The single plant of spontaneously doubled haploid carries out artificial chromosome and doubles；After the monoploid regrowth bolting, by its single plant bagging
Selfing, single plant acquire seed；Breeding objective season is taken to sow, the excellent pure lines of screening integral economic trait.
Specifically, the method for haploid breeding are as follows:
1) prepared by explant: for conventional new varieties or the breeding of hybrid new breed male parent system, selection meets breeding objective
Complementary type breeding material stripping flower bud emasculation hybridization, obtain the filial generation of radish, sow its filial generation, taken to bolting Post flowering
Its anther is as explant；For male sterile line, that is, hybrid new breed female parent system breeding, selection meets showing for breeding objective
There are male sterility maintainer line and the stripping flower bud emasculation hybridization of corresponding complementary type breeding material, obtains the filial generation of radish, it is miscellaneous to sow its
Offspring is handed over, takes its anther as explant to bolting Post flowering；
2) haploid induction: the anther as explant is inoculated on radish haploid induction culture medium, is 33 in temperature
Then dark culture 1~2 day under conditions of~38 DEG C is transferred under conditions of temperature is 20~28 DEG C dark culture to haploid embryo
Until shape body occurs；
3) regeneration culture: being placed in optical culture under conditions of temperature is 20~28 DEG C for the monoploid embryoid turned out,
It is transferred to radish after the greening of monoploid embryoid to sprout on culture medium, hereafter every 25~30 days subcultures are primary, the sliding samming when five days
The differentiated monoploid regrowth of bud eye out is transferred in radish root media when degree is lower than 28 DEG C and continues to develop；
4) crop field transplanting and chromosome doubling: the good monoploid regrowth of root system development is moved into field production, works as length
Its blade lower epidermis microscopy is taken when 3~5 true leaves out, its ploidy is determined, to the list that spontaneously doubled haploid does not occur
Strain carries out artificial chromosome and doubles；
5) isolation pollination: after the monoploid regrowth bolting in big field, divided single plant bagging to be selfed, single plant is divided to acquire
Seed；
6) economic characters are screened: seed obtained is selfed to single plant bagging, respectively takes and is sowed on a small quantity in breeding objective season,
It observes and filters out the excellent pure lines of integral economic trait；
7) the later period procedure of breeding: for conventional breeding of new variety, the recovery-type for taking above-mentioned integral economic trait excellent is pure
System, expanding propagation can be obtained the new quality product kind of radish；For hybrid new breed breeding, take above-mentioned integral economic trait excellent
Maintenance and male parent type pure lines, artificial to shell the combination of flower bud emasculation preparing hybrid, obtained filial generation is after planting combined power measurement,
The combination strong for specific combining ability takes maintenance therein to be sheerly edge sterile line close with its and carries out mostly generation backcrossing, select phase
The sterile line answered, the above-mentioned excellent combined sterile line of mass propagation and male parent system, can be obtained radish newly excellent Hybrid.
In some specific embodiments of the invention, the pH value of the induced medium is 5.8~6.0, component with
Mass parts meter includes:
In some specific embodiments of the invention, the component of the induced medium includes: in terms of mass parts
In some specific embodiments of the invention, the pH value of the culture medium of sprouting is 5.5~5.6, component with
Mass parts meter includes:
In some specific embodiments of the invention, the pH value of the root media is 5.5~5.6, component with
Mass parts meter includes:
In some specific embodiments of the invention, condition that the condition of culture of the induced medium is 33~38 DEG C
Lower dark culture 1~2 day is transferred under conditions of temperature is 20~28 DEG C until dark culture to monoploid embryoid appearance.
In some specific embodiments of the invention, the temperature of the optical culture is 20~28 DEG C.
In some specific embodiments of the invention, the culture obtains the monoploid regrowth for differentiating bud eye, will
The monoploid regrowth is transferred to that root media specially every 25~30 days subcultures of taking root are primary, and sliding equal temperature was lower than when five days
The differentiated monoploid regrowth of bud eye out, which is transferred in radish root media, at 28 DEG C continues to develop.
The present invention formulates a series of evening a kind of sedge radish DH systems using radish haploid breeding technology；Male is carried out to the DH system of acquisition
Sterile retentivity measurement, is then to take turns the parent that wanders, transformation DH male sterile line with maintenance DH system；It is obtained by breeding objective from above-mentioned
A collection of candidate material is picked out in the initiative material obtained, and is female parent with DH male sterile line, and DH system is male parent, is combined power
Measurement, filters out advantage cross combination.Success of the radish haploid breeding technology provided by the invention in commercial applications, has
Effect alleviates the situation of spring white radishes seed heavy dependence import.
Import spring white radishes kind carries out monoploid culture after hybridizing with domestic spring white radishes local varieties, chooses evening therein
A kind of sedge white radishes DH system is combined power measurement, obtains excellent combination.Such polymerization mix excellent base of domestic and international spring white radishes
Cause shows the excellent production advantage.
White jade spring, day great spring are that South Korea produces spring white radishes kind, and bolting is extremely late, the long cylindricality of fleshy root, white skin plain boiled pork, radish
It is lightly seasoned.The plum blossom spring is the local spring white radishes kind in Guangdong, and in Guangdong Province's January, earlier bolting phenomenon, endoplasm will not occur for sowing
Root jar shape, white skin plain boiled pork, the flavor with Chinese radish.The Guangdong spring is not always the local spring white radishes kind in Guangdong Province, in Guangdong
Earlier bolting phenomenon will not be occurred by saving January sowing, endoplasm root long cylindricality and cherry mouthful smaller (like bottleneck), white skin plain boiled pork have
The flavor of Chinese radish.
Male parent ED5168 has polymerize the advantages of not old Guangdong spring and day letter spring, and bolting resistant property is close with the day great spring, radish flavor
Uneducated person is not close with the Guangdong spring, and the fleshy root length spring greater than day is slightly short.
Maternal ED0108A has polymerize the advantages of plum blossom spring and white jade spring, and bolting resistant property is close with the white jade spring, radish flavor with
The plum blossom spring is close, and fleshy root shape and length are close with the white jade spring.
The successful breeding and commercial applications of middle trailing plants No.1 as domestic spring white radishes replacement kind, further enrich
The kind deposit of domestic spring white radishes enhances the whole synthesis competitiveness of domestic import spring white radishes substitute species, for domestic radish
Breeding team completes spring white radishes seed import substitution process and enhances technical strength.
Specific embodiment
The invention discloses a kind of breeding method of radish, those skilled in the art can use for reference present disclosure, be suitably modified
Realization of process parameters.In particular, it should be pointed out that all similar substitutions and modifications be for a person skilled in the art it is aobvious and
It is clear to, they are considered as being included in the present invention.Method and application of the invention is retouched by preferred embodiment
It states, related personnel can obviously not depart from the content of present invention, change in spirit and scope to method described herein and application
Dynamic or appropriate changes and combinations, carry out implementation and application the technology of the present invention.
Raw materials used, auxiliary material and reagent are available on the market in the breeding method of radish provided by the invention.
Below with reference to embodiment, the present invention is further explained:
1 haploid breeding of embodiment
1) prepared by explant: for conventional new varieties or the breeding of hybrid new breed male parent system, selection meets breeding objective
Complementary type breeding material stripping flower bud emasculation hybridization, obtain the filial generation of radish, sow its filial generation, taken to bolting Post flowering
Its anther is as explant；For male sterile line, that is, hybrid new breed female parent system breeding, selection meets showing for breeding objective
There are male sterility maintainer line and the stripping flower bud emasculation hybridization of corresponding complementary type breeding material, obtains the filial generation of radish, it is miscellaneous to sow its
Offspring is handed over, takes its anther as explant to bolting Post flowering；
2) haploid induction: the anther as explant is inoculated on radish haploid induction culture medium, is 33 in temperature
Then dark culture 1~2 day under conditions of~38 DEG C is transferred under conditions of temperature is 20~28 DEG C dark culture to haploid embryo
Until shape body occurs；
3) regeneration culture: being placed in optical culture under conditions of temperature is 20~28 DEG C for the monoploid embryoid turned out,
It is transferred to radish after the greening of monoploid embryoid to sprout on culture medium, hereafter every 25~30 days subcultures are primary, the sliding samming when five days
The differentiated monoploid regrowth of bud eye out is transferred in radish root media when degree is lower than 28 DEG C and continues to develop；
4) crop field transplanting and chromosome doubling: the good monoploid regrowth of root system development is moved into field production, works as length
Its blade lower epidermis microscopy is taken when 3~5 true leaves out, its ploidy is determined, to the list that spontaneously doubled haploid does not occur
Strain carries out artificial chromosome and doubles；
5) isolation pollination: after the monoploid regrowth bolting in big field, divided single plant bagging to be selfed, single plant is divided to acquire
Seed；
6) economic characters are screened: seed obtained is selfed to single plant bagging, respectively takes and is sowed on a small quantity in breeding objective season,
It observes and filters out the excellent pure lines of integral economic trait；
7) the later period procedure of breeding: for conventional breeding of new variety, the recovery-type for taking above-mentioned integral economic trait excellent is pure
System, expanding propagation can be obtained the new quality product kind of radish；For hybrid new breed breeding, take above-mentioned integral economic trait excellent
Maintenance and male parent type pure lines, artificial to shell the combination of flower bud emasculation preparing hybrid, obtained filial generation is after planting combined power measurement,
The combination strong for specific combining ability takes maintenance therein to be sheerly edge sterile line close with its and carries out mostly generation backcrossing, select phase
The sterile line answered, the above-mentioned excellent combined sterile line of mass propagation and male parent system, can be obtained radish newly excellent Hybrid.
The pH value of induced medium is 5.8~6.0 comprising (mg/L):
Sprout culture medium pH value be 5.5~5.6 comprising (mg/L):
The pH value of root media is 5.5~5.6 comprising (mg/L):
2 haploid breeding of embodiment
1) prepared by explant: for conventional new varieties or the breeding of hybrid new breed male parent system, selection meets breeding objective
Complementary type breeding material stripping flower bud emasculation hybridization, obtain the filial generation of radish, sow its filial generation, taken to bolting Post flowering
Its anther is as explant；For male sterile line, that is, hybrid new breed female parent system breeding, selection meets showing for breeding objective
There are male sterility maintainer line and the stripping flower bud emasculation hybridization of corresponding complementary type breeding material, obtains the filial generation of radish, it is miscellaneous to sow its
Offspring is handed over, takes its anther as explant to bolting Post flowering；
2) haploid induction: the anther as explant is inoculated on radish haploid induction culture medium, is 33 in temperature
Then dark culture 1~2 day under conditions of~38 DEG C is transferred under conditions of temperature is 20~28 DEG C dark culture to haploid embryo
Until shape body occurs；
3) regeneration culture: being placed in optical culture under conditions of temperature is 20~28 DEG C for the monoploid embryoid turned out,
It is transferred to radish after the greening of monoploid embryoid to sprout on culture medium, hereafter every 25~30 days subcultures are primary, the sliding samming when five days
The differentiated monoploid regrowth of bud eye out is transferred in radish root media when degree is lower than 28 DEG C and continues to develop；
4) crop field transplanting and chromosome doubling: the good monoploid regrowth of root system development is moved into field production, works as length
Its blade lower epidermis microscopy is taken when 3~5 true leaves out, its ploidy is determined, to the list that spontaneously doubled haploid does not occur
Strain carries out artificial chromosome and doubles；
5) isolation pollination: after the monoploid regrowth bolting in big field, divided single plant bagging to be selfed, single plant is divided to acquire
Seed；
6) economic characters are screened: seed obtained is selfed to single plant bagging, respectively takes and is sowed on a small quantity in breeding objective season,
It observes and filters out the excellent pure lines of integral economic trait；
7) the later period procedure of breeding: for conventional breeding of new variety, the recovery-type for taking above-mentioned integral economic trait excellent is pure
System, expanding propagation can be obtained the new quality product kind of radish；For hybrid new breed breeding, take above-mentioned integral economic trait excellent
Maintenance and male parent type pure lines, artificial to shell the combination of flower bud emasculation preparing hybrid, obtained filial generation is after planting combined power measurement,
The combination strong for specific combining ability takes maintenance therein to be sheerly edge sterile line close with its and carries out mostly generation backcrossing, select phase
The sterile line answered, the above-mentioned excellent combined sterile line of mass propagation and male parent system, can be obtained radish newly excellent Hybrid.
Induced medium includes (mg/L):
Sprout culture medium pH value be 5.5~5.6 comprising (mg/L):
The pH value of root media is 5.5~5.6 comprising (mg/L):
3 haploid breeding of embodiment
1) prepared by explant: for conventional new varieties or the breeding of hybrid new breed male parent system, selection meets breeding objective
Complementary type breeding material stripping flower bud emasculation hybridization, obtain the filial generation of radish, sow its filial generation, taken to bolting Post flowering
Its anther is as explant；For male sterile line, that is, hybrid new breed female parent system breeding, selection meets showing for breeding objective
There are male sterility maintainer line and the stripping flower bud emasculation hybridization of corresponding complementary type breeding material, obtains the filial generation of radish, it is miscellaneous to sow its
Offspring is handed over, takes its anther as explant to bolting Post flowering；
2) haploid induction: the anther as explant is inoculated on radish haploid induction culture medium, is 33 in temperature
Then dark culture 1~2 day under conditions of~38 DEG C is transferred under conditions of temperature is 20~28 DEG C dark culture to haploid embryo
Until shape body occurs；
3) regeneration culture: being placed in optical culture under conditions of temperature is 20~28 DEG C for the monoploid embryoid turned out,
It is transferred to radish after the greening of monoploid embryoid to sprout on culture medium, hereafter every 25~30 days subcultures are primary, the sliding samming when five days
The differentiated monoploid regrowth of bud eye out is transferred in radish root media when degree is lower than 28 DEG C and continues to develop；
4) crop field transplanting and chromosome doubling: the good monoploid regrowth of root system development is moved into field production, works as length
Its blade lower epidermis microscopy is taken when 3~5 true leaves out, its ploidy is determined, to the list that spontaneously doubled haploid does not occur
Strain carries out artificial chromosome and doubles；
5) isolation pollination: after the monoploid regrowth bolting in big field, divided single plant bagging to be selfed, single plant is divided to acquire
Seed；
6) economic characters are screened: seed obtained is selfed to single plant bagging, respectively takes and is sowed on a small quantity in breeding objective season,
It observes and filters out the excellent pure lines of integral economic trait；
7) the later period procedure of breeding: for conventional breeding of new variety, the recovery-type for taking above-mentioned integral economic trait excellent is pure
System, expanding propagation can be obtained the new quality product kind of radish；For hybrid new breed breeding, take above-mentioned integral economic trait excellent
Maintenance and male parent type pure lines, artificial to shell the combination of flower bud emasculation preparing hybrid, obtained filial generation is after planting combined power measurement,
The combination strong for specific combining ability takes maintenance therein to be sheerly edge sterile line close with its and carries out mostly generation backcrossing, select phase
The sterile line answered, the above-mentioned excellent combined sterile line of mass propagation and male parent system, can be obtained radish newly excellent Hybrid.
The pH value of induced medium is 5.8~6.0 comprising (mg/L):
Sprout culture medium pH value be 5.5~5.6 comprising (mg/L):
The pH value of root media is 5.5~5.6 comprising (mg/L):
The breeding of 4 radish new varieties of embodiment
(1) " plum blossom spring radish " is female parent, " white jade spring radish " is male parent, prepares complementary combinations, is provided using Examples 1 to 3
Radish haploid breeding technology formulate 928, DH system, then through field character overall merit obtain 9 excellent DH systems, to this 9
Excellent DH system carries out the measurement of male sterility retentivity, obtain 4, maintenance DH system (ED0108, ED0126, ED0152,
ED0198), then with this four DH system is kept (to refer to the parent in backcrossing for recurrent parent.Backcrossing refers to that a parents produce
Raw hybrid hybridizes with one of parent again.Generally when hybridizing first time, choosing has the kind work of good characteristic maternal, and
Make male parent when each backcrossing afterwards, this parent is recurrent parent in backcrossing) obtain DH male sterile line four
(ED0108A、ED0126A、ED0152A、ED0198A)。
(2) it is maternal, " day letter spring radish " for male parent with " Guangdong spring not old radish ", prepares complementary combinations, utilize embodiment 1
~3 radish haploid breeding technologies provided formulate 836, DH system, then obtain 10 excellent DH systems through field character overall merit
(ED5168, ED5236, ED5238, ED5265, ED5279, ED5398, ED5628, ED5789, ED5796, ED5863), uses this
A little DH systems are as male parent material.
(3) take above-mentioned two coordinate force stronger evening bolting male sterile line ED0108A, ED0198A to make maternal, with above-mentioned
The acquisition of acquisition 10 parts of DH pure lines ED5168, ED5236, ED5238, ED5265, ED5279, ED5398, ED5628,
ED5789, ED5796, ED5863 make male parent system, carry out diallel cross, combine in spring preparing hybrid in 2010, altogether with combination 20
It is a, combining ability test test and multiple spot variety comparative test for 2011-2012.
1 cross combination table of table
| ED0108A×ED5168 | ED0108A×ED5236 | ED0108A×ED5265 | ED0108A×ED5279 |
| ED0108A×ED5398 | ED0108A×ED5398 | ED0108A×ED5628 | ED0108A×ED5789 |
| ED0108A×ED5796 | ED0108A×ED5863 | ED0198A×ED5168 | ED0198A×ED5236 |
| ED0198A×ED5265 | ED0198A×ED5279 | ED0198A×ED5398 | ED0198A×ED5398 |
| ED0198A×ED5628 | ED0198A×ED5789 | ED0198A×ED5796 | ED0198A×ED5863 |
To the combination of above-mentioned 20 trials carry out take turns variety comparative tests, the optimum combination filtered out be ED0108A ×
ED5168。
1. trial combines the stubble variety comparative test of high mountain autumn in 2010
Test material: the diallel cross offspring of above-mentioned spring white radishes material is control with white light.
Test site: the big mountain top village in Enshi City slab bridge town.
Test method: random district's groups arrangement, in triplicate, plot area 13.3m2, line-spacing 30cm, spacing in the rows 20cm, July 25
Day sowing, outdoor cropping.
Test result: it is shown in Table 1.
From Table 2, it can be seen that the stubble trial combination of high mountain autumn in 2010 ED0108A × ED5168, ED0198A ×
ED5398、ED0108A×ED5236、ED0198A×ED5265、ED0108A×ED5398、ED0198A×ED5279、
ED0108A×ED5628、ED0198A×ED5628、ED0108A×ED5279、ED0198A×ED5789、ED0108A×
ED5789 yield be higher than control, wherein combination ED0108A × ED5168, ED0198A × ED5398, ED0108A × ED5236,
ED0198A × ED5265, ED0108A × ED5398, ED0198A × ED5279 reach significant difference with compareing；Combine ED0108A
× ED5168, ED0198A × ED5398, ED0108A × ED5236, ED0198A × ED5265 reaches extremely significant difference with compareing
(p < 0.01).
2 trial of table combines the stubble Yield comparison of high mountain autumn in 2010
Difference.The stubble combination of high mountain autumn in 2010 is combined according to trial and filters out four optimum combinations than test result, respectively
Are as follows: ED0108A × ED5168, ED0198A × ED5398, ED0108A × ED5236, ED0198A × ED5265.These combinations produce
Amount is higher than control and reaches significant difference.
2. trial combines the stubble variety comparative test of Plain early spring in 2011
Test material: the diallel cross offspring of above-mentioned spring white radishes material is control with white light.
Test site: Jingzhou City town Li Bu village Yang Jing
Test method: random district's groups arrangement, in triplicate, plot area 13.3m2, line-spacing 30cm, spacing in the rows 20cm, 2 months 28
Day sowing, outdoor cropping.
Test result: 3 are shown in Table.
From table 3 it is observed that Plain early spring in 2011 combination ED0108A × ED5168, ED0198A × ED5265,
ED0108A×ED5628、ED0198A×ED5398、ED0108A×ED5398、ED0198A×ED5279、ED0108A×
ED5236, ED0198A × ED5628, ED0108A × ED5279, ED0198A × ED5789, ED0108A × ED5398 yield are high
In control, wherein combination ED0108A × ED5168, ED0198A × ED5265, ED0108A × ED5628, ED0198A ×
ED5398, ED0108A × ED5398 reach significant difference with compareing；Combine ED0108A × ED5168, ED0198A × ED5265,
ED0108A × ED5628, ED0198A × ED5398 reach extremely significant difference with compareing.
Plain early spring variety comparative test result in 2011 is combined according to trial and filters out four optimum combinations, is respectively as follows:
ED0108A×ED5168,ED0198A×ED5265,ED0108A×ED5628,ED0198A×ED5398.These combination yield
Higher than compareing and reach significant difference.
Merge the stubble of high mountain autumn in 2008 and each 4 optimum combinations of stubble of Plain spring in 2009, obtain 5 excellent combinations:
ED0108A×ED5168、ED0198A×ED5398、ED0198A×ED5265、ED0108A×ED5236、ED0108A×
ED5628。
3 trial of table combines Plain early spring Yield comparison in 2011
3. the stubble combination comparative test of high mountain summer in 2011
Test material: ED0108A × ED5168, ED0198A × ED5398, ED0198A × ED5265, ED0108A ×
The excellent combination of ED5236, ED0108A × ED5628 five is participated in the experiment, and is control with white light.
Test site: the big mountain top village in Enshi City slab bridge town.
Test method: random district's groups arrangement, in triplicate, plot area 13.3m2, line-spacing 30cm, spacing in the rows 20cm, May 10
Day sowing, plastic film mulching cultivation.
Test result: 4 are shown in Table.
Table 4
| Combination | I | Ⅱ | Ⅲ | Xi | P0.05 | P0.01 |
| ED0108A×ED5168 | 158 | 160 | 160 | 159.3 | a | A |
| ED0198A×ED5398 | 153 | 155 | 156 | 153.7 | b | B |
| ED0198A×ED5265 | 151 | 154 | 152 | 152.3 | bc | BC |
| ED0108A×ED5236 | 148 | 150 | 150 | 149.3 | c | BC |
| ED0108A×ED5628 | 147 | 148 | 146 | 147.0 | c | C |
| White light | 135 | 138 | 142 | 138.3 | d | D |
4. the stubble combination comparative test of high mountain autumn in 2011
Test material: ED0108A × ED5168, ED0198A × ED5398, ED0198A × ED5265, ED0108A ×
The excellent combination of ED5236, ED0108A × ED5628 five is participated in the experiment, and is control with white light.
Test site: the big mountain top village in Enshi City slab bridge town.
Test method: random district's groups arrangement, in triplicate, plot area 13.3m2, line-spacing 30cm, spacing in the rows 20cm, July 10
Day sowing, outdoor cropping.
Test result: 5 are shown in Table
The stubble combination comparative test of 5 2011 years high mountain autumns of table
| Combination | I | Ⅱ | Ⅲ | Xi | P0.05 | P0.01 |
| ED0108A×ED5168 | 169 | 171 | 171 | 170.3 | a | A |
| ED0198A×ED5265 | 165 | 167 | 167 | 165.3 | b | B |
| ED0198A×ED5398 | 162 | 165 | 163 | 163.3 | bc | BC |
| ED0108A×ED5236 | 158 | 160 | 160 | 159.3 | c | C |
| ED0108A×ED5628 | 158 | 158 | 156 | 157.3 | c | C |
| White light | 145 | 149 | 152 | 148.7 | d | D |
From table 4, it can be seen that the stubble ED0108A × ED5168 of high mountain autumn in 2001, ED0198A × ED5265, ED0198A ×
ED5398, ED0108A × ED5236, ED0108A × ED5628 yield be higher than control, wherein combination ED0108A × ED5168 with
ED0198A × ED5265 reaches extremely significant difference,
ED0198A × ED5265 and ED0198A × ED5398 difference is not significant.
5. the stubble variety comparative test of Plain early spring in 2012
Test material: ED0108A × ED5168, ED0198A × ED5398, ED0198A × ED5265, ED0108A ×
The excellent combination of ED5236, ED0108A × ED5628 five is participated in the experiment, and is control with white light.
Test site: Jingzhou City town Li Bu village Yang Jing
Test method: random district's groups arrangement, in triplicate, plot area 13.3m2, line-spacing 30cm, spacing in the rows 20cm, 2 months 28
Day sowing, outdoor cropping.
6 2012 years Plain early springs of table stubble combination Yield comparison
| Combination | I | Ⅱ | Ⅲ | Xi | P0.05 | P0.01 |
| ED0108A×ED5168 | 143 | 145 | 145 | 144.3 | a | A |
| ED0198A×ED5398 | 138 | 140 | 141 | 139.7 | b | B |
| ED0198A×ED5265 | 136 | 139 | 137 | 137.3 | bc | BC |
| ED0108A×ED5236 | 133 | 135 | 135 | 134.3 | c | BC |
| ED0108A×ED5628 | 132 | 133 | 131 | 132.0 | c | C |
| White light | 121 | 123 | 127 | 123.7 | d | D |
As can be seen from Table 6, Plain early spring in 2010 stubble ED0108A × ED5168,
ED0198A × ED5398, ED0198A × ED5265, ED0108A × ED5236, ED0108A × ED5628 yield are high
In control, wherein combination ED0108A × ED5168 and ED0198A × ED5398 reach extremely significant difference, ED0198A × ED5398
It is not significant with ED0198A × ED5265 difference.
It can from the stubble of high mountain summer in 2011, the stubble of high mountain autumn in 2011 and the stubble combination comparative test of Plain early spring in 2012
Out, ED0108A × ED5168, ED0198A × ED5398, ED0198A × ED5265 performance are good and stable.
6. the stubble combination comparative test of high mountain summer in 2012
Test material: tri- ED0108A × ED5168, ED0198A × ED5398, ED0198A × ED5265 excellent combinations
It participates in the experiment, is control with white light.
Test site: the big mountain top village in Enshi City slab bridge town.
Test method: random district's groups arrangement, in triplicate, plot area 13.3m2, line-spacing 30cm, spacing in the rows 20cm, May 10
Day sowing, plastic film mulching cultivation.
Test result: 7 are shown in Table
7 2012 years high mountain summers of table stubble combination Yield comparison
As can be seen from Table 7, the high mountain summer in 2010 stubble ED0108A × ED5168, ED0198A × ED5398, ED0198A ×
ED5265 yield be higher than control, wherein combination ED0108A × ED5168 with
ED0198A × ED5398 reaches extremely significant difference, and ED0198A × ED5398 and ED0198A × ED5265 difference are not
Significantly.
7. the stubble combination comparative test of high mountain autumn in 2012
Test material: tri- ED0108A × ED5168, ED0198A × ED5398, ED0198A × ED5265 excellent combinations
It participates in the experiment, is control with white light.
Test site: the big mountain top village in Enshi City slab bridge town.
Test method: random district's groups arrangement, in triplicate, plot area 13.3m2, line-spacing 30cm, spacing in the rows 20cm, July 10
Day sowing, outdoor cropping.
Test result: 8 are shown in Table.
As can be seen from Table 8, the high mountain summer in 2012 stubble ED0108A × ED5168, ED0198A × ED5265, ED0198A ×
ED5398 yield be higher than control, wherein combination ED0108A × ED5168 with
ED0198A × ED5265 reaches extremely significant difference, and E ED0198A × ED5265 and D0198A × ED5398 difference are not
Significantly.
8 2012 years high mountain autumns of table stubble combination Yield comparison
| Combination | I | Ⅱ | Ⅲ | Xi | P0.05 | P0.01 |
| ED0108A×ED5168 | 155 | 150 | 161 | 155.3 | a | A |
| ED0198A×ED5265 | 139 | 140 | 137 | 138.3 | b | B |
| ED0198A×ED5398 | 134 | 130 | 129 | 131.0 | b | B |
| White light | 130 | 126 | 130 | 128.7 | c | c |
8. the stubble combination comparative test of Plain early spring in 2013
Test material: tri- ED0108A × ED5168, ED0198A × ED5398, ED0198A × ED5265 excellent combinations
It participates in the experiment, is control with white light.
Test site: Jingzhou City town Li Bu village Yang Jing
Test method: random district's groups arrangement, in triplicate, plot area 13.3m2, line-spacing 30cm, spacing in the rows 20cm, 2 months 28
Day sowing, outdoor cropping.
9 2013 years Plain early springs of table stubble combination Yield comparison
| Combination | I | Ⅱ | Ⅲ | Xi | P0.05 | P0.01 |
| ED0108A×ED5168 | 141 | 135 | 146 | 140.7 | a | A |
| ED0198A×ED5398 | 124 | 125 | 122 | 123.3 | b | B |
| ED0198A×ED5265 | 119 | 115 | 114 | 116.0 | b | B |
| White light | 115 | 111 | 115 | 113.7 | c | c |
As can be seen from Table 9, the stubble of Plain early spring in 2013 ED0108A × ED5168, ED0198A × ED5398, ED0198A
× ED5265 yield is higher than control, wherein combination ED0108A × ED5168 and ED0198A × ED5398 reach extremely significant difference, E
D0198A × ED5398 and ED0198A × ED5265 difference is not significant.
It can be with from the stubble of high mountain summer in 2012, the stubble of high mountain autumn in 2012 and the stubble combination of Plain early spring in 2013 comparative test result
Find out that ED0108A × ED5168 performance is most excellent, determines that it is selected combination, and preliminary designation is middle trailing plants No.1.
(5) ED0108A × ED5168 has carried out Regional suitability test in multiple radish main producing regions, be significantly better than it is existing into
Mouth main breed, registers through crops validation board of Hunan Province, names as " middle trailing plants No.1 ".
2013 have five points, five variety comparative tests altogether in crossdrift, Yanling County, Sangzhi, Jing Zhou, Jun Mountain, are control with white light, converge
Overall result is shown in Table 10.
Trailing plants No.1 multispots trial yield result table in 2013 in table 10
Note: plot area is 16.7 square metres.
As known from Table 10, the single number yield in five points in 2013 reaches extremely significant volume increase than control in, average to increase production
Up to 16.18%, and the peasant for planting experimentally ground reflects that uniformity is good, and commodity is good.
2014, in 5 crossdrift, Yanling County, Sangzhi, Jing Zhou, Jun Mountain variety comparative tests, are control with white light, as a result summarize and be shown in Table
11。
Single number multispots trial yield result table in 2014 in table 11
Note: plot area is 16.7 square metres.
As known from Table 11, the single number yield in five points in 2014 reaches extremely significant volume increase than control in, average to increase production
Up to 17.70%, and the peasant for planting experimentally ground reflects that uniformity is good, and commodity is good.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (9)
1. breeding method, which comprises the steps of:
Step 1: being maternal, " white jade spring radish " for male parent with " plum blossom spring radish ", DH system is obtained by haploid breeding；
Step 2: the excellent DH system of selection Agronomic characteristic overall merit measures male sterility retentivity, obtains maintenance DH
System obtains DH male sterile line using maintenance DH system as recurrent parent；
Step 3: being maternal, " day letter spring radish " for male parent with " Guangdong spring not old radish ", DH system is obtained by haploid breeding；Choosing
The excellent DH pure lines of Agronomic characteristic overall merit are selected as male parent, using the DH male sterile line that step 2 obtains as
Female parent, carries out diallel cross, and combined power measurement obtains advantage cross combination.
2. breeding method as described in claim 1, which is characterized in that the haploid breeding specifically: after obtaining explant
It is inoculated in induced medium, until culture to monoploid embryoid occurs；By the monoploid embryoid after optical culture is afforested
It is transferred to culture medium of sprouting, culture obtains the monoploid regrowth for differentiating bud eye, the monoploid regrowth is transferred to and is taken root
Culture medium is taken root, then moves into field production, its blade microscopy is taken when growing 3~5 true leaves, determines its ploidy, right
The single plant that spontaneously doubled haploid does not occur carries out artificial chromosome and doubles；After the monoploid regrowth bolting, by its list
Strain bagging selfing, single plant acquire seed；Breeding objective season is taken to sow, the excellent pure lines of screening integral economic trait.
3. breeding method as claimed in claim 2, which is characterized in that the pH value of the induced medium is 5.8~6.0,
Component includes: in terms of mass parts
4. breeding method as claimed in claim 2, which is characterized in that the component of the induced medium is wrapped in terms of mass parts
It includes:
5. breeding method as claimed in claim 2, which is characterized in that the pH value of the culture medium of sprouting is 5.5~5.6,
Component includes: in terms of mass parts
6. breeding method as claimed in claim 2, which is characterized in that the pH value of the root media is 5.5~5.6,
Component includes: in terms of mass parts
7. such as the described in any item breeding methods of claim 2 to 6, which is characterized in that the condition of culture of the induced medium
Dark culture 1~2 day under conditions of being 33~38 DEG C, dark culture is transferred under conditions of temperature is 20~28 DEG C to haploid embryo
Until shape body occurs.
8. such as the described in any item breeding methods of claim 2 to 7, which is characterized in that the temperature of the optical culture is 20~28
℃。
9. such as the described in any item breeding methods of claim 2 to 8, which is characterized in that the culture acquisition differentiates bud eye
It is primary to be transferred to root media specially every 25~30 days subcultures of taking root by monoploid regrowth for the monoploid regrowth, when
The monoploid regrowth of differentiated bud eye out when the equal temperature of cunning is lower than 28 DEG C on the five is transferred to radish root media relaying supervention
It educates.
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Non-Patent Citations (2)
| Title |
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| 李丹: "萝卜游离小孢子培养技术研究", 《中国优秀硕士学位论文全文数据库农业科技辑》 * |
| 袁伟玲等: "春白萝卜新品种雪单3 号的选育", 《中国蔬菜》 * |
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