CN109220544A - A kind of preparation method of Antrodia camphorata solid culture dry powder - Google Patents
A kind of preparation method of Antrodia camphorata solid culture dry powder Download PDFInfo
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- CN109220544A CN109220544A CN201811099637.5A CN201811099637A CN109220544A CN 109220544 A CN109220544 A CN 109220544A CN 201811099637 A CN201811099637 A CN 201811099637A CN 109220544 A CN109220544 A CN 109220544A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/50—Inoculation of spawn
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
- A01G18/69—Arrangements for managing the environment, e.g. sprinklers
Abstract
The invention belongs to technical field of biological culture, especially a kind of preparation method of Antrodia camphorata solid culture dry powder, specifically includes the following steps: Antrodia camphorata strain is inoculated in slant medium by (1), culture obtains mycelium inclined-plane；(2) mycelium inclined plane inoculating is placed on magnetic stirring apparatus in the triangular flask for filling fluid nutrient medium, cultivates, obtains Mycelium culture liquid；(3) Mycelium culture liquid is inoculated with, fermented and cultured, strain seed fermentation is mature, obtains strain liquid；(4) strain liquid is inoculated with, is cultivated, liquid fungus seed is completed, and mycelium fermentation broth is obtained；(5) mycelium fermentation broth is inoculated with, cultivates, obtains Antrodia camphorata solid culture, dried, crushed, obtain Antrodia camphorata solid culture dry powder；Formula is scientific and reasonable for culture medium use, full of nutrition, so that bacterium germination speed is fast, with short production cycle, so that the Contents of Main Components such as polysaccharide body, total triterpene and adenosine are abundant in fructification, sesame is neat out, yield is high.
Description
Technical field
The invention belongs to technical field of biological culture, especially a kind of preparation method of Antrodia camphorata solid culture dry powder.
Background technique
Antrodia camphorata abbreviation antrodia, popular name cinnamomum kanahirai hay mushroom, red antrodia, mushroom, camphor tree mushroom, blood ganoderma lucidum etc. in camphor tree, wild Antrodia camphorata is unique
The host to depend on for existence is Taiwan cinnamomum kanehirai, and cinnamomum kanehirai is the exclusive primary rare tree in Taiwan, is grown in height above sea level 450 ~ 2000
Between meter；Antrodia camphorata is grown on the moist surface of the rotten hollow inside of aged trunk or the trunk that lodges, and quantity is very rare,
And the speed of growth is very slow, is made laws and is worth much high for national protected trees since rare numbers, acquisition are not easy
In ganoderma lucidum, ginseng, referred to as the best in medicinal material；It is Antrodia camphorata gas fragrance, acrid flavour, hardship, flat, containing there are many physiological activity at
Point, such as polysaccharide body, triterpene compound, adenosine have protect liver, anticancer, adjust immune, disallergization, removing toxic substances and the function such as anti-inflammatory
Effect is considered as unique and precious medicinal fungi, refreshing sesame, the ruby in forest, has high research and commercial value.
Summary of the invention
In view of the above-mentioned problems, the present invention is intended to provide a kind of preparation method of Antrodia camphorata solid culture dry powder.
The invention is realized by the following technical scheme:
A kind of preparation method of Antrodia camphorata solid culture dry powder, specifically includes the following steps:
(1) Antrodia camphorata strain is inoculated in slant medium, constant temperature incubation 4 ~ 5 days under conditions of temperature is 21 ~ 23 DEG C, in temperature
Degree is to obtain mycelium inclined-plane constant temperature incubation 4 ~ 5 days under conditions of 26 ~ 28 DEG C；
The slant medium, formula are as follows: 700 ~ 710mL of potato juice, 180 ~ 190mL of tomato juice, 21 ~ 22g of glucose, egg
White 12 ~ 13g of peptone, 1.3 ~ 1.4g of magnesium sulfate, 3.1 ~ 3.2g of potassium dihydrogen phosphate, 21 ~ 22g of agar；
(2) by mycelium inclined plane inoculating in the 1000mL triangular flask for filling 700mL fluid nutrient medium, be placed in revolving speed be 1400 ~
It is 26 ~ 28 DEG C in temperature constant temperature incubation 4 ~ 5 days under conditions of temperature is 21 ~ 23 DEG C on the magnetic stirring apparatus of 1500r/min
Under conditions of constant temperature incubation 4 ~ 5 days, obtain Mycelium culture liquid；
The fluid nutrient medium, formula are as follows: soybean powder 0.8% ~ 0.9%, purple sweet potato powder 0.32% ~ 0.38%, cornstarch 0.32% ~
0.38%, potato starch 0.32% ~ 0.38%, glucose 1.1% ~ 1.3%, sucrose 1.1% ~ 1.3%, potassium dihydrogen phosphate 0.10% ~
0.12%, magnesium sulfate 0.05% ~ 0.06%, vitamin B10.003% ~ 0.004%, surplus is water；
(3) Mycelium culture liquid is inoculated with by seeding tank seed culture by inoculum concentration 16% ~ 18%, and 26 ~ 28 DEG C of temperature continuous black
Dark condition, 220 ~ 240r/min of mixing speed, filtrated air ventilatory capacity be 1:0.62 ~ 0.66v/v per minute, tank pressure 34 ~
38kPa, fermented and cultured 4 ~ 5 days, pH was down to 3.2 ~ 3.4, and strain seed fermentation is mature, obtained strain liquid；
(4) strain liquid is inoculated with by fermentation tank culture by inoculum concentration 16% ~ 18%, 26 ~ 28 DEG C of temperature, mixing speed be 220 ~
240r/min, filtrated air ventilatory capacity are 1:0.53 ~ 0.57v/v per minute, and tank presses 46 ~ 50kPa, are cultivated 6 ~ 7 days, pH is down to
3.2 ~ 3.4, liquid fungus seed is completed, and obtains mycelium fermentation broth；
(5) mycelium fermentation broth is inoculated with, 26 ~ 28 DEG C of temperature, humidity 50% by solid fermentation culture by inoculum concentration 16% ~ 18%
~ 55%, continuous darkness condition, daily ventilation, antrodia strain well-grown on cereal materials culture medium, in growth course
Mycelium switchs to pink by white, and is tightly wrapped cereal materials particle, and late stage of culture mycelium color is slightly partially yellow,
Outside is wrapped in antrodia camphorata mycelium film, and part even forms vacuum, gos deep into inside feed particles, cultivates 90 ~ 100 days, obtains Antrodia camphorata
Solid culture is dried, and is crushed, is obtained Antrodia camphorata solid culture dry powder；
The solid medium, is made of raw material from the following weight: 26 ~ 28 parts of wheat, 10 ~ 11 parts of corn, sweet cherry roots 32 ~
34 parts, 0.24 ~ 0.28 part of pueraria polysaccharide, 0.13 ~ 0.15 part of vitexicarpin, 0.19 ~ 0.21 part of curcumin, stevioside 0.54 ~
0.58 part, 0.24 ~ 0.28 part of algal polysaccharides.
As invention further embodiment: tomato juice described in step (1) is prepared according to the following steps: by tomato and
1.4 ~ 1.6 times of its weight amounts, the ethanol solution that mass fraction is 7% ~ 8% are mixed with beating, under conditions of merging temperature is 30 ~ 32 DEG C
80 ~ 90h of constant-temperature enclosed fermentation, mild fire are heated to temperature and are 89 ~ 93 DEG C and are stirred continuously, hyperfiltration, takes filtrate, tomato is added
The momordica grosvenori polysaccharide of 0.03 ~ 0.04 times of weight amount is stirred to dissolution, obtains tomato juice.
As invention further embodiment: purple sweet potato powder described in step (2) is prepared: according to the following steps by purple sweet potato powder
Broken, constant temperature toasts 5 ~ 6min in the baking box that merging temperature is 37 ~ 39 DEG C, is warming up to the heating rate of 2.7 ~ 2.9 DEG C/min
81 ~ 83 DEG C of continuation constant temperature toast 7 ~ 8min, take out, and merging temperature is constant temperature 35 ~ 40min of cooling treatment under conditions of 3 ~ 4 DEG C,
Magnetic field strength is 6 ~ 8min of magnetization treatment under conditions of 220 ~ 230G, magnetization treatment under conditions of magnetic field strength is 760 ~ 770G
8 ~ 10min obtains purple sweet potato powder.
As invention further embodiment: solid medium described in step (5) is prepared: will be small according to the following steps
Wheat, corn and sweet cherry roots co-grinding, merging temperature are constant temperature 18 ~ 22min of frying under conditions of 54 ~ 58 DEG C, pulverize, add
Enter 2.3 ~ 2.5 times of wheat weight amounts, temperature to stir evenly for 44 ~ 48 DEG C of water, constant temperature is close under conditions of temperature is 34 ~ 36 DEG C
60 ~ 70h of fermentation is closed, pueraria polysaccharide, vitexicarpin, curcumin, stevioside and algal polysaccharides are added and stir evenly, obtains solid training
Support base.
As invention further embodiment: a kind of Antrodia camphorata solid of the preparation method preparation of Antrodia camphorata solid culture dry powder
Cultivate dry powder.
Beneficial effects of the present invention: a kind of preparation method of Antrodia camphorata solid culture dry powder provided by the invention, culture medium
Formula is scientific and reasonable for use, full of nutrition, can satisfy culture propagation nutritional need, improves Antrodia camphorata bacterial activity, promotes
The effective Differentiation and proliferation of strain, so that strain well-grown on culture medium, bacterium germination speed is fast, with short production cycle, also
Culture medium effective component is absorbed and utilized in the pollution that miscellaneous bacteria can effectively be avoided, the middle mycelium that speeds growth process, and promotes mycelia
The Nutrition and Metabolism efficiency of body promotes the conversion and accumulation of metabolite, promotes the quick and a large amount of formation of Antrodia camphorata fructification, makes
It obtains the Contents of Main Components such as polysaccharide body, total triterpene and adenosine in fructification to enrich, sesame is neat out, yield is high, effectively solution Cinnamomum kanahirai hay
The difficult problem of sesame culture, and healthy nutritive value and economic value are high.
Specific embodiment
Illustrate the present invention with specific embodiment below, but is not limitation of the present invention.
Embodiment 1
In the embodiment of the present invention, a kind of preparation method of Antrodia camphorata solid culture dry powder, specifically includes the following steps:
(1) Antrodia camphorata strain is inoculated in slant medium, constant temperature incubation 4 ~ 5 days under conditions of temperature is 21 DEG C, in temperature
Constant temperature incubation 4 ~ 5 days under conditions of being 26 DEG C, mycelium inclined-plane is obtained；
The slant medium, formula are as follows: potato juice 700mL, tomato juice 180mL, glucose 21g, peptone 12g, sulphur
Sour magnesium 1.3g, potassium dihydrogen phosphate 3.1g, agar 21g；
(2) by mycelium inclined plane inoculating in the 1000mL triangular flask for filling 700mL fluid nutrient medium, being placed in revolving speed is 1400r/
On the magnetic stirring apparatus of min, constant temperature incubation 4 ~ 5 days under conditions of temperature is 21 DEG C, constant temperature under conditions of temperature is 26 DEG C
Culture 4 ~ 5 days, obtains Mycelium culture liquid；
The fluid nutrient medium, formula are as follows: soybean powder 0.8%, purple sweet potato powder 0.32%, cornstarch 0.32%, potato starch
0.32%, glucose 1.1%, sucrose 1.1%, potassium dihydrogen phosphate 0.10%, magnesium sulfate 0.05%, vitamin B10.003%, surplus is
Water；
(3) Mycelium culture liquid is inoculated with by seeding tank seed culture by inoculum concentration 16%, 26 DEG C of continuous darkness conditions of temperature,
Mixing speed 220r/min, filtrated air ventilatory capacity are 1:0.62v/v per minute, and tank presses 34kPa, and fermented and cultured 4 ~ 5 days, pH dropped
To 3.2 ~ 3.4, strain seed fermentation is mature, obtains strain liquid；
(4) strain liquid is inoculated with, 26 DEG C of temperature, mixing speed 220r/min, nothing by fermentation tank culture by inoculum concentration 16%
Bacterium air vent amount is 1:0.53v/v per minute, and tank presses 46kPa, is cultivated 6 ~ 7 days, pH is down to 3.2 ~ 3.4, liquid fungus seed
It completes, obtains mycelium fermentation broth；
(5) mycelium fermentation broth is inoculated with by solid fermentation culture by inoculum concentration 16%, 26 DEG C of temperature, humidity 50%, continuous black
Dark condition, daily ventilation cultivate 90 ~ 100 days, obtain Antrodia camphorata solid culture, dry, and crush, and obtain the training of Antrodia camphorata solid
Support dry powder；
The solid medium, is made of raw material from the following weight: 26 parts of wheat, 10 parts of corn, 32 parts of sweet cherry roots, pueraria lobata
0.24 part of polysaccharide, 0.13 part of vitexicarpin, 0.19 part of curcumin, 0.54 part of stevioside, 0.24 part of algal polysaccharides.
As invention further embodiment: tomato juice described in step (1) is prepared according to the following steps: by tomato and
1.4 times of its weight amounts, the ethanol solution that mass fraction is 7% are mixed with beating, and merging temperature is constant-temperature enclosed hair under conditions of 30 DEG C
Ferment 80h, mild fire are heated to temperature and are 89 DEG C and are stirred continuously, hyperfiltration, takes filtrate, sieve of 0.03 times of tomato weight amount is added
Chinese fruit polysaccharide is stirred to dissolution, obtains tomato juice.
As invention further embodiment: purple sweet potato powder described in step (2) is prepared: according to the following steps by purple sweet potato powder
Broken, constant temperature toasts 5min in the baking box that merging temperature is 37 DEG C, and it is permanent to be warming up to 81 DEG C of continuation with the heating rate of 2.7 DEG C/min
Temperature baking 7min, takes out, and merging temperature is constant temperature cooling treatment 35min under conditions of 3 DEG C, in the condition that magnetic field strength is 220G
Lower magnetization treatment 6min, magnetization treatment 8min under conditions of magnetic field strength is 760G, obtains purple sweet potato powder.
As invention further embodiment: solid medium described in step (5) is prepared: will be small according to the following steps
Wheat, corn and sweet cherry roots co-grinding, merging temperature are constant temperature frying 18min under conditions of 54 DEG C, pulverize, wheat is added
2.3 times of weight amounts, the water that temperature is 44 DEG C stir evenly, and Pueraria lobota is added in constant-temperature enclosed fermentation 60h under conditions of temperature is 34 DEG C
Root polysaccharide, vitexicarpin, curcumin, stevioside and algal polysaccharides stir evenly, and obtain solid medium.
As invention further embodiment: a kind of Antrodia camphorata solid of the preparation method preparation of Antrodia camphorata solid culture dry powder
Cultivate dry powder.
Embodiment 2
In the embodiment of the present invention, a kind of preparation method of Antrodia camphorata solid culture dry powder, specifically includes the following steps:
(1) Antrodia camphorata strain is inoculated in slant medium, constant temperature incubation 4 ~ 5 days under conditions of temperature is 22 DEG C, in temperature
Constant temperature incubation 4 ~ 5 days under conditions of being 27 DEG C, mycelium inclined-plane is obtained；
The slant medium, formula are as follows: potato juice 705mL, tomato juice 185mL, glucose 21.5g, peptone
12.5g, magnesium sulfate 1.35g, potassium dihydrogen phosphate 3.15g, agar 21.5g；
(2) by mycelium inclined plane inoculating in the 1000mL triangular flask for filling 700mL fluid nutrient medium, being placed in revolving speed is 1450r/
On the magnetic stirring apparatus of min, constant temperature incubation 4 ~ 5 days under conditions of temperature is 22 DEG C, constant temperature under conditions of temperature is 27 DEG C
Culture 4 ~ 5 days, obtains Mycelium culture liquid；
The fluid nutrient medium, formula are as follows: soybean powder 0.85%, purple sweet potato powder 0.35%, cornstarch 0.35%, potato starch
0.35%, glucose 1.2%, sucrose 1.2%, potassium dihydrogen phosphate 0.11%, magnesium sulfate 0.055%, vitamin B10.0035%, surplus is
Water；
(3) Mycelium culture liquid is inoculated with by seeding tank seed culture by inoculum concentration 17%, 27 DEG C of continuous darkness conditions of temperature,
Mixing speed 230r/min, filtrated air ventilatory capacity are 1:0.64v/v per minute, and tank presses 36kPa, and fermented and cultured 4 ~ 5 days, pH dropped
To 3.2 ~ 3.4, strain seed fermentation is mature, obtains strain liquid；
(4) strain liquid is inoculated with, 27 DEG C of temperature, mixing speed 230r/min, nothing by fermentation tank culture by inoculum concentration 17%
Bacterium air vent amount is 1:0.55v/v per minute, and tank presses 48kPa, is cultivated 6 ~ 7 days, pH is down to 3.2 ~ 3.4, liquid fungus seed
It completes, obtains mycelium fermentation broth；
(5) mycelium fermentation broth is inoculated with by solid fermentation culture by inoculum concentration 17%, 27 DEG C of temperature, humidity 53%, continuous black
Dark condition, daily ventilation cultivate 90 ~ 100 days, obtain Antrodia camphorata solid culture, dry, and crush, and obtain the training of Antrodia camphorata solid
Support dry powder；
The solid medium, is made of raw material from the following weight: 27 parts of wheat, 10.5 parts of corn, 33 parts of sweet cherry roots, Pueraria lobota
0.26 part of root polysaccharide, 0.14 part of vitexicarpin, 0.2 part of curcumin, 0.56 part of stevioside, 0.26 part of algal polysaccharides.
As invention further embodiment: tomato juice described in step (1) is prepared according to the following steps: by tomato and
1.5 times of its weight amounts, the ethanol solution that mass fraction is 7.5% are mixed with beating, and merging temperature is constant temperature under conditions of 30 ~ 32 DEG C
Sealed fermenting 85h, mild fire are heated to temperature and are 91 DEG C and are stirred continuously, hyperfiltration, takes filtrate, tomato weight 0.035 is added
The momordica grosvenori polysaccharide of amount is stirred to dissolution again, obtains tomato juice.
As invention further embodiment: purple sweet potato powder described in step (2) is prepared: according to the following steps by purple sweet potato powder
Broken, constant temperature toasts 5.5min in the baking box that merging temperature is 38 DEG C, is warming up to 81 ~ 83 DEG C with the heating rate of 2.8 DEG C/min
Continue constant temperature and toast 7.5min, take out, merging temperature is constant temperature cooling treatment 38min under conditions of 3.5 DEG C, is in magnetic field strength
Magnetization treatment 7min under conditions of 225G, magnetization treatment 9min under conditions of magnetic field strength is 765G, obtains purple sweet potato powder.
As invention further embodiment: solid medium described in step (5) is prepared: will be small according to the following steps
Wheat, corn and sweet cherry roots co-grinding, merging temperature are constant temperature frying 20min under conditions of 56 DEG C, pulverize, wheat is added
2.4 times of weight amounts, the water that temperature is 46 DEG C stir evenly, and Pueraria lobota is added in constant-temperature enclosed fermentation 65h under conditions of temperature is 35 DEG C
Root polysaccharide, vitexicarpin, curcumin, stevioside and algal polysaccharides stir evenly, and obtain solid medium.
As invention further embodiment: a kind of Antrodia camphorata solid of the preparation method preparation of Antrodia camphorata solid culture dry powder
Cultivate dry powder.
Embodiment 3
In the embodiment of the present invention, a kind of preparation method of Antrodia camphorata solid culture dry powder, specifically includes the following steps:
(1) Antrodia camphorata strain is inoculated in slant medium, constant temperature incubation 4 ~ 5 days under conditions of temperature is 23 DEG C, in temperature
Constant temperature incubation 4 ~ 5 days under conditions of being 28 DEG C, mycelium inclined-plane is obtained；
The slant medium, formula are as follows: potato juice 710mL, tomato juice 190mL, glucose 22g, peptone 13g, sulphur
Sour magnesium 1.4g, potassium dihydrogen phosphate 3.2g, agar 22g；
(2) by mycelium inclined plane inoculating in the 1000mL triangular flask for filling 700mL fluid nutrient medium, being placed in revolving speed is 1500r/
On the magnetic stirring apparatus of min, constant temperature incubation 4 ~ 5 days under conditions of temperature is 23 DEG C, constant temperature under conditions of temperature is 28 DEG C
Culture 4 ~ 5 days, obtains Mycelium culture liquid；
The fluid nutrient medium, formula are as follows: soybean powder 0.9%, purple sweet potato powder 0.38%, cornstarch 0.38%, potato starch
0.38%, glucose 1.3%, sucrose 1.3%, potassium dihydrogen phosphate 0.12%, magnesium sulfate 0.06%, vitamin B10.004%, surplus is
Water；
(3) Mycelium culture liquid is inoculated with by seeding tank seed culture by inoculum concentration 18%, 28 DEG C of continuous darkness conditions of temperature,
Mixing speed 240r/min, filtrated air ventilatory capacity are 1:0.66v/v per minute, and tank presses 38kPa, and fermented and cultured 4 ~ 5 days, pH dropped
To 3.2 ~ 3.4, strain seed fermentation is mature, obtains strain liquid；
(4) strain liquid is inoculated with, 28 DEG C of temperature, mixing speed 240r/min, nothing by fermentation tank culture by inoculum concentration 18%
Bacterium air vent amount is 1:0.57v/v per minute, and tank presses 50kPa, is cultivated 6 ~ 7 days, pH is down to 3.2 ~ 3.4, liquid fungus seed
It completes, obtains mycelium fermentation broth；
(5) mycelium fermentation broth is inoculated with by solid fermentation culture by inoculum concentration 18%, 28 DEG C of temperature, humidity 55%, continuous black
Dark condition, daily ventilation cultivate 90 ~ 100 days, obtain Antrodia camphorata solid culture, dry, and crush, and obtain the training of Antrodia camphorata solid
Support dry powder；
The solid medium, is made of raw material from the following weight: 28 parts of wheat, 11 parts of corn, 34 parts of sweet cherry roots, pueraria lobata
0.28 part of polysaccharide, 0.15 part of vitexicarpin, 0.21 part of curcumin, 0.58 part of stevioside, 0.28 part of algal polysaccharides.
As invention further embodiment: tomato juice described in step (1) is prepared according to the following steps: by tomato and
1.6 times of its weight amounts, the ethanol solution that mass fraction is 8% are mixed with beating, and merging temperature is constant-temperature enclosed hair under conditions of 32 DEG C
Ferment 90h, mild fire are heated to temperature and are 93 DEG C and are stirred continuously, hyperfiltration, takes filtrate, sieve of 0.04 times of tomato weight amount is added
Chinese fruit polysaccharide is stirred to dissolution, obtains tomato juice.
As invention further embodiment: purple sweet potato powder described in step (2) is prepared: according to the following steps by purple sweet potato powder
Broken, constant temperature toasts 6min in the baking box that merging temperature is 39 DEG C, and it is permanent to be warming up to 83 DEG C of continuation with the heating rate of 2.9 DEG C/min
Temperature baking 8min, takes out, and merging temperature is constant temperature cooling treatment 40min under conditions of 3 ~ 4 DEG C, in the item that magnetic field strength is 230G
Magnetization treatment 8min under part, magnetization treatment 10min under conditions of magnetic field strength is 770G, obtains purple sweet potato powder.
As invention further embodiment: solid medium described in step (5) is prepared: will be small according to the following steps
Wheat, corn and sweet cherry roots co-grinding, merging temperature are constant temperature frying 22min under conditions of 58 DEG C, pulverize, wheat is added
2.5 times of weight amounts, the water that temperature is 48 DEG C stir evenly, and Pueraria lobota is added in constant-temperature enclosed fermentation 70h under conditions of temperature is 36 DEG C
Root polysaccharide, vitexicarpin, curcumin, stevioside and algal polysaccharides stir evenly, and obtain solid medium.
As invention further embodiment: a kind of Antrodia camphorata solid of the preparation method preparation of Antrodia camphorata solid culture dry powder
Cultivate dry powder.
Comparative example 1
A kind of preparation method of Antrodia camphorata solid culture dry powder, the difference from embodiment 1 is that, by tomato described in step (1)
Juice is replaced with potato juice, and other conditions are all the same.
Comparative example 2
A kind of preparation method of Antrodia camphorata solid culture dry powder, the difference from embodiment 1 is that, by purple sweet potato described in step (2)
Powder is replaced with soybean powder, and other conditions are all the same.
Comparative example 3
A kind of preparation method of Antrodia camphorata solid culture dry powder, the difference from embodiment 1 is that, the training of solid described in step (5)
It supports in base and is added without pueraria polysaccharide and vitexicarpin, other conditions are all the same.
Comparative example 4
A kind of preparation method of Antrodia camphorata solid culture dry powder, the difference from embodiment 1 is that, the training of solid described in step (5)
It supports in base and is added without curcumin, stevioside and algal polysaccharides, other conditions are all the same.
Antrodia camphorata solid culture dry powder is made using the preparation method of embodiment and comparative example in same a collection of Antrodia camphorata strain,
Its polysaccharide body, total triterpene and adenosine Contents of Main Components are detected, testing result such as table 1:
1 embodiment and comparative example Antrodia camphorata solid culture dry powder Contents of Main Components of table compares (unit: g/100g dry weight)
As it can be seen from table 1 the main components such as Antrodia camphorata solid culture dry powder polysaccharide body, total triterpene and adenosine produced by the present invention
Rich content, and content is apparently higher than comparative example.
Claims (5)
1. a kind of preparation method of Antrodia camphorata solid culture dry powder, which is characterized in that specifically includes the following steps:
(1) Antrodia camphorata strain is inoculated in slant medium, constant temperature incubation 4 ~ 5 days under conditions of temperature is 21 ~ 23 DEG C, in temperature
Degree is to obtain mycelium inclined-plane constant temperature incubation 4 ~ 5 days under conditions of 26 ~ 28 DEG C；
The slant medium, formula are as follows: 700 ~ 710mL of potato juice, 180 ~ 190mL of tomato juice, 21 ~ 22g of glucose, egg
White 12 ~ 13g of peptone, 1.3 ~ 1.4g of magnesium sulfate, 3.1 ~ 3.2g of potassium dihydrogen phosphate, 21 ~ 22g of agar；
(2) by mycelium inclined plane inoculating in the 1000mL triangular flask for filling 700mL fluid nutrient medium, be placed in revolving speed be 1400 ~
It is 26 ~ 28 DEG C in temperature constant temperature incubation 4 ~ 5 days under conditions of temperature is 21 ~ 23 DEG C on the magnetic stirring apparatus of 1500r/min
Under conditions of constant temperature incubation 4 ~ 5 days, obtain Mycelium culture liquid；
The fluid nutrient medium, formula are as follows: soybean powder 0.8% ~ 0.9%, purple sweet potato powder 0.32% ~ 0.38%, cornstarch 0.32% ~
0.38%, potato starch 0.32% ~ 0.38%, glucose 1.1% ~ 1.3%, sucrose 1.1% ~ 1.3%, potassium dihydrogen phosphate 0.10% ~
0.12%, magnesium sulfate 0.05% ~ 0.06%, vitamin B10.003% ~ 0.004%, surplus is water；
(3) Mycelium culture liquid is inoculated with by seeding tank seed culture by inoculum concentration 16% ~ 18%, and 26 ~ 28 DEG C of temperature continuous black
Dark condition, 220 ~ 240r/min of mixing speed, filtrated air ventilatory capacity be 1:0.62 ~ 0.66v/v per minute, tank pressure 34 ~
38kPa, fermented and cultured 4 ~ 5 days, pH was down to 3.2 ~ 3.4, and strain seed fermentation is mature, obtained strain liquid；
(4) strain liquid is inoculated with by fermentation tank culture by inoculum concentration 16% ~ 18%, 26 ~ 28 DEG C of temperature, mixing speed be 220 ~
240r/min, filtrated air ventilatory capacity are 1:0.53 ~ 0.57v/v per minute, and tank presses 46 ~ 50kPa, are cultivated 6 ~ 7 days, pH is down to
3.2 ~ 3.4, liquid fungus seed is completed, and obtains mycelium fermentation broth；
(5) mycelium fermentation broth is inoculated with, 26 ~ 28 DEG C of temperature, humidity 50% by solid fermentation culture by inoculum concentration 16% ~ 18%
~ 55%, continuous darkness condition, daily ventilation cultivate 90 ~ 100 days, obtain Antrodia camphorata solid culture, dry, and crush, obtain ox
Antrodia solid culture dry powder；
The solid medium, is made of raw material from the following weight: 26 ~ 28 parts of wheat, 10 ~ 11 parts of corn, sweet cherry roots 32 ~
34 parts, 0.24 ~ 0.28 part of pueraria polysaccharide, 0.13 ~ 0.15 part of vitexicarpin, 0.19 ~ 0.21 part of curcumin, stevioside 0.54 ~
0.58 part, 0.24 ~ 0.28 part of algal polysaccharides.
2. the preparation method of Antrodia camphorata solid culture dry powder according to claim 1, which is characterized in that step (1) is described
Tomato juice, prepared according to the following steps: by tomato and 1.4 ~ 1.6 times of its weight amount, mass fraction be 7% ~ 8% ethyl alcohol it is molten
Liquid is mixed with beating, and merging temperature is 80 ~ 90h of constant-temperature enclosed fermentation under conditions of 30 ~ 32 DEG C, and it is 89 ~ 93 that mild fire, which is heated to temperature,
DEG C and be stirred continuously, hyperfiltration, take filtrate, the momordica grosvenori polysaccharide that 0.03 ~ 0.04 times of tomato weight amount is added is stirred to dissolution,
Obtain tomato juice.
3. the preparation method of Antrodia camphorata solid culture dry powder according to claim 1, which is characterized in that step (2) is described
Purple sweet potato powder, prepared according to the following steps: purple sweet potato crushed, merging temperature be constant temperature baking 5 in 37 ~ 39 DEG C of baking box ~
6min is warming up to 81 ~ 83 DEG C of continuation constant temperature with the heating rate of 2.7 ~ 2.9 DEG C/min and toasts 7 ~ 8min, takes out, and merging temperature is
35 ~ 40min of constant temperature cooling treatment under conditions of 3 ~ 4 DEG C, 6 ~ 8min of magnetization treatment under conditions of magnetic field strength is 220 ~ 230G,
8 ~ 10min of magnetization treatment under conditions of magnetic field strength is 760 ~ 770G, obtains purple sweet potato powder.
4. the preparation method of Antrodia camphorata solid culture dry powder according to claim 1, which is characterized in that step (5) is described
Solid medium, prepared according to the following steps: by wheat, corn and sweet cherry roots co-grinding, being placed in temperature is 54 ~ 58 DEG C
Under conditions of constant temperature 18 ~ 22min of frying, pulverize, 2.3 ~ 2.5 times of wheat weight amounts be added, temperature is that 44 ~ 48 DEG C of water stirs
It mixes uniformly, pueraria polysaccharide, vitexicarpin, turmeric is added in 60 ~ 70h of constant-temperature enclosed fermentation under conditions of temperature is 34 ~ 36 DEG C
Element, stevioside and algal polysaccharides stir evenly, and obtain solid medium.
5. the Antrodia camphorata solid of the preparation method preparation of described in any item Antrodia camphorata solid culture dry powder according to claim 1 ~ 4
Cultivate dry powder.
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