CN109197592A - A method of reducing albumen mulberry tissue culture Vitrification - Google Patents
A method of reducing albumen mulberry tissue culture Vitrification Download PDFInfo
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- CN109197592A CN109197592A CN201811245473.2A CN201811245473A CN109197592A CN 109197592 A CN109197592 A CN 109197592A CN 201811245473 A CN201811245473 A CN 201811245473A CN 109197592 A CN109197592 A CN 109197592A
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- culture
- albumen mulberry
- tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention relates to a kind of methods for reducing albumen mulberry tissue culture Vitrification, specifically includes the following steps: (1) tissue culture albumen mulberry, (2) adjust culture medium, reduce hormone in medium, (3) temperature is adjusted: temperature, (4) Illumination adjusting between reduction culture, (5) extend the domestication time.The invention has the advantages that: provide a kind of method for reducing albumen mulberry tissue culture Vitrification, the probability of albumen mulberry tissue culture Vitrification is effectively reduced, the yield that albumen mulberry can preferably be improved solves the problems, such as that existing albumen mulberry demand is implacable, and replicability is strong.
Description
Technical field
The present invention relates to albumen mulberry tissue culture technology field, and in particular to a kind of to reduce albumen mulberry tissue culture Vitrification
Method.
Background technique
Albumen mulberry also cries feed mulberry, is Beijing Sang Liang Technical Development Center scientist in 1998 from national 28 mulberry tree product
The heterosis, hybrid vigor mulberry selected in kind, the sub- albumen of albumen mulberry leaf are up to 36%, and branch albumen reaches 28%, and leaf is edible
Fresh vegetables, mulberry tea be also can be the good merchantable brand of beautifying face and moistering lotion with the pure natural product of lower hyperlipidemia, hypertension, hyperglycemia, mulberry powder.Because of albumen mulberry nutriture value
Value is high, is used for feed, medicine, vegetables are widely used, and demand is big.It, throughout the year can be big since tissue culture is not controlled by season
Amount breeding, in albumen mulberry tissue culture, test tube seedling is easy vitrifying, so affecting tissue-cultured seedling reproduction speed and quality.Tissue culture egg
Bai Sang overcomes vitrifying, just solves the problems, such as to encounter in tissue culture, vitrifying is how to reduce egg the phenomenon that frequently encountering in tissue culture
White mulberry tissue culture Vitrification becomes new research direction.
Summary of the invention
The purpose of the present invention is to provide a kind of methods for reducing albumen mulberry tissue culture Vitrification, to solve above-mentioned back
The problem of being proposed in scape technology.
In order to solve the above technical problems, technical solution provided by the invention are as follows: a kind of reduction albumen mulberry tissue culture test tube seedling glass
The method of glass, specifically includes the following steps:
(1) tissue culture albumen mulberry: the healthy and strong disease-free albumen mulberry of selection, taking its stem section with axillary bud is explant, by outer
Sterile system is established in implant disinfection, by adding the optimization of hormone concentration to subculture medium, filters out the proliferation training of albumen mulberry
Base is supported, into expansion numerous stage；
(2) culture medium is adjusted, hormone in medium is reduced: reducing the content of 6-BA in MS culture medium, is guaranteeing fast numerous matter
Amount with 3-4 times of multiple in the case of basic element of cell division 6-BA become 1mg/L, and the active carbon of addition 0.01% from 2mg/L；
(3) temperature is adjusted: reducing the temperature between culture；
(4) Illumination adjusting: if there is using between the culture of natural lighting, temperature is controlled at 25-29 DEG C, and natural lighting does not surpass
7000 luxs are crossed, humidity is no more than 40%, and the big increase pollution rate of humidity, natural lighting is reinforced, original vitrified young plant
Slowly restore normal, between the culture of no natural lighting, the illumination of lamp stick is increased to 5000 luxs from 3000 luxs；
(5) extend the domestication time: extending the domestication time when domestication, increase domestication illumination.
As a preferred embodiment, subculture medium is 6-BA concentration 2mg/L, NAA concentration 0.1mg/ in the step (1)
The MS culture medium of L.
The invention has the advantages that: a kind of method for reducing albumen mulberry tissue culture Vitrification is provided, egg is effectively reduced
The probability of white mulberry tissue culture Vitrification can preferably improve the yield of albumen mulberry, and it is difficult to solve existing albumen mulberry demand
The problem of to meet, replicability are strong.
Specific embodiment
Illustrate the present invention with specific embodiment below, is not limitation of the present invention.
Embodiment 1
A method of albumen mulberry tissue culture Vitrification is reduced, specifically includes the following steps:
(1) tissue culture albumen mulberry: the healthy and strong disease-free albumen mulberry of selection, taking its stem section with axillary bud is explant, by outer
Sterile system is established in implant disinfection, by adding the optimization of hormone concentration to subculture medium, filters out the proliferation training of albumen mulberry
Base is supported, into expanding numerous stage, the subculture medium is the MS culture medium of 6-BA concentration 2mg/L, NAA concentration 0.1mg/L；
(2) culture medium is adjusted, hormone in medium is reduced: reducing the content of 6-BA in MS culture medium, is guaranteeing fast numerous matter
Amount with 3-4 times of multiple in the case of basic element of cell division 6-BA become 1mg/L, and the active carbon of addition 0.01% from 2mg/L；
(3) temperature is adjusted: reducing the temperature between culture；
(4) Illumination adjusting: if there is using between the culture of natural lighting, temperature is controlled at 25 DEG C, and natural lighting is no more than
5000 luxs, humidity are no more than 40%, and the big increase pollution rate of humidity, natural lighting is reinforced, and original vitrified young plant is also slow
It is slow to restore normal, between the culture of no natural lighting, the illumination of lamp stick is increased to 5000 luxs from 3000 luxs；
(5) extend the domestication time: extending the domestication time when domestication, increase domestication illumination.
Embodiment 2
A method of albumen mulberry tissue culture Vitrification is reduced, specifically includes the following steps:
(1) tissue culture albumen mulberry: the healthy and strong disease-free albumen mulberry of selection, taking its stem section with axillary bud is explant, by outer
Sterile system is established in implant disinfection, by adding the optimization of hormone concentration to subculture medium, filters out the proliferation training of albumen mulberry
Base is supported, into expanding numerous stage, the subculture medium is the MS culture medium of 6-BA concentration 2mg/L, NAA concentration 0.1mg/L；
(2) culture medium is adjusted, hormone in medium is reduced: reducing the content of 6-BA in MS culture medium, is guaranteeing fast numerous matter
Amount with 3-4 times of multiple in the case of basic element of cell division 6-BA become 1mg/L, and the active carbon of addition 0.01% from 2mg/L；
(3) temperature is adjusted: reducing the temperature between culture；
(4) Illumination adjusting: if there is using between the culture of natural lighting, temperature is controlled at 27 DEG C, and natural lighting is no more than
6000 luxs, humidity are no more than 40%, and the big increase pollution rate of humidity, natural lighting is reinforced, and original vitrified young plant is also slow
It is slow to restore normal, between the culture of no natural lighting, the illumination of lamp stick is increased to 5000 luxs from 3000 luxs；
(5) extend the domestication time: extending the domestication time when domestication, increase domestication illumination.
Embodiment 3
A method of albumen mulberry tissue culture Vitrification is reduced, specifically includes the following steps:
(1) tissue culture albumen mulberry: the healthy and strong disease-free albumen mulberry of selection, taking its stem section with axillary bud is explant, by outer
Sterile system is established in implant disinfection, by adding the optimization of hormone concentration to subculture medium, filters out the proliferation training of albumen mulberry
Base is supported, into expanding numerous stage, the subculture medium is the MS culture medium of 6-BA concentration 2mg/L, NAA concentration 0.1mg/L；
(2) culture medium is adjusted, hormone in medium is reduced: reducing the content of 6-BA in MS culture medium, is guaranteeing fast numerous matter
Amount with 3-4 times of multiple in the case of basic element of cell division 6-BA become 1mg/L, and the active carbon of addition 0.01% from 2mg/L；
(3) temperature is adjusted: reducing the temperature between culture；
(4) Illumination adjusting: if there is using between the culture of natural lighting, temperature is controlled at 29 DEG C, and natural lighting is no more than
7000 luxs, humidity are no more than 40%, and the big increase pollution rate of humidity, natural lighting is reinforced, and original vitrified young plant is also slow
It is slow to restore normal, between the culture of no natural lighting, the illumination of lamp stick is increased to 5000 luxs from 3000 luxs；
(5) extend the domestication time: extending the domestication time when domestication, increase domestication illumination.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (2)
1. a kind of method for reducing albumen mulberry tissue culture Vitrification, which is characterized in that specifically includes the following steps:
(1) tissue culture albumen mulberry: the healthy and strong disease-free albumen mulberry of selection, taking its stem section with axillary bud is explant, by explant
Sterile system is established in disinfection, by the optimization to subculture medium addition hormone concentration, filters out the proliferated culture medium of albumen mulberry,
Into expansion numerous stage；
(2) adjust culture medium, reduce hormone in medium: reducing the content of 6-BA in MS culture medium, guarantee fast numerous quality with
Basic element of cell division 6-BA is become 1mg/L from 2mg/L in the case of 3-4 times of multiple, and 0.01% active carbon is added；
(3) temperature is adjusted: reducing the temperature between culture；
(4) Illumination adjusting: if there is using between the culture of natural lighting, temperature is controlled at 25-29 DEG C, and natural lighting is no more than
7000 luxs, humidity are no more than 40%, and the big increase pollution rate of humidity, natural lighting is reinforced, and original vitrified young plant is also slow
It is slow to restore normal, between the culture of no natural lighting, the illumination of lamp stick is increased to 5000 luxs from 3000 luxs；
(5) extend the domestication time: extending the domestication time when domestication, increase domestication illumination.
2. a kind of method for reducing albumen mulberry tissue culture Vitrification according to claim 1, it is characterised in that: described
Subculture medium is the MS culture medium of 6-BA concentration 2mg/L, NAA concentration 0.1mg/L in step (1).
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0322932A (en) * | 1989-06-19 | 1991-01-31 | Mitsui Petrochem Ind Ltd | Formation and proliferation of crown of plant of genus asparagus and proliferation of seedling |
| CN102283128A (en) * | 2011-07-21 | 2011-12-21 | 中国热带农业科学院南亚热带作物研究所 | Method for overcoming vitrification phenomenon of sisal hemp tissue culture seedlings |
| CN106234212A (en) * | 2015-12-07 | 2016-12-21 | 内蒙古自治区农牧业科学研究院 | A kind of method for tissue culture controlling Fructus Fragariae Ananssae Vitrification |
| CN107318650A (en) * | 2017-07-25 | 2017-11-07 | 贵州海铭巍杂交构树产业开发有限公司 | A kind of nutrient solution for paper mulberry tissue cultures |
| CN109329054A (en) * | 2018-10-18 | 2019-02-15 | 广东海洋大学 | A kind of vitrified method of reduction mulberries adventitious bud |
| CN109964799A (en) * | 2019-04-03 | 2019-07-05 | 湖北省农业科学院果树茶叶研究所 | A method of hybridization paper mulberry tissue-cultured seedling solid strong plantlets and rootage |
-
2018
- 2018-10-24 CN CN201811245473.2A patent/CN109197592A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0322932A (en) * | 1989-06-19 | 1991-01-31 | Mitsui Petrochem Ind Ltd | Formation and proliferation of crown of plant of genus asparagus and proliferation of seedling |
| CN102283128A (en) * | 2011-07-21 | 2011-12-21 | 中国热带农业科学院南亚热带作物研究所 | Method for overcoming vitrification phenomenon of sisal hemp tissue culture seedlings |
| CN106234212A (en) * | 2015-12-07 | 2016-12-21 | 内蒙古自治区农牧业科学研究院 | A kind of method for tissue culture controlling Fructus Fragariae Ananssae Vitrification |
| CN107318650A (en) * | 2017-07-25 | 2017-11-07 | 贵州海铭巍杂交构树产业开发有限公司 | A kind of nutrient solution for paper mulberry tissue cultures |
| CN109329054A (en) * | 2018-10-18 | 2019-02-15 | 广东海洋大学 | A kind of vitrified method of reduction mulberries adventitious bud |
| CN109964799A (en) * | 2019-04-03 | 2019-07-05 | 湖北省农业科学院果树茶叶研究所 | A method of hybridization paper mulberry tissue-cultured seedling solid strong plantlets and rootage |
Non-Patent Citations (4)
| Title |
|---|
| MARIA DOLORES ARIAS PADRO ET AL.: "Cryopreservation of white mulberry (Morus alba L.) by encapsulation-dehydration and vitrification", 《PLANT CELL TISS ORGAN CULT》 * |
| 胡孔峰 等: "《植物组织培养技术及应用》", 31 March 2006, 河南科学技术出版社 * |
| 计东风 等: "桑树快速繁殖应用技术的研究", 《桑蚕通报》 * |
| 陈兵先 等: "植物组织培养试管苗玻璃化现象研究进展", 《林业科技开发》 * |
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