CN109197383A - A kind of Pleurotus eryngii culture medium and Pleurotus eryngii inoculation method - Google Patents
A kind of Pleurotus eryngii culture medium and Pleurotus eryngii inoculation method Download PDFInfo
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- CN109197383A CN109197383A CN201811073553.4A CN201811073553A CN109197383A CN 109197383 A CN109197383 A CN 109197383A CN 201811073553 A CN201811073553 A CN 201811073553A CN 109197383 A CN109197383 A CN 109197383A
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- pleurotus eryngii
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- Agricultural Chemicals And Associated Chemicals (AREA)
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Abstract
The invention discloses a kind of Pleurotus eryngii culture medium and Pleurotus eryngii inoculation methods, including chitosan 6-8 parts by weight, diatomite 1.5-1.8 parts by weight, amino polyphosphazene microspheres 0.5-0.8 parts by weight, wheat straw 42-45 parts by weight, rice husk 30-32 parts by weight, dregs of beans 8-10.3 parts by weight, specific inoculation method is as follows: step 1: chitosan, diatomite, amino polyphosphazene microspheres, wheat straw, rice husk and dregs of beans being pulverized and mixed, sieving is carried out by sieve, guarantees its density size；Step 2: water is added in the compost after step 1 is mixed, and carries out 3-5 turning, is formulated as the culture medium that pH value is 7-7.5, moisture content has water to dissociate with finger grip, and does not drip as standard.Culture cost of material is lower, and technique is simpler, and its high organic content, nutrition covering scope are big, keep the Pleurotus eryngii turned out fertile, rich in protein abundant, calcium, phosphorus and vitamin etc..
Description
Technical field
The invention belongs to mushroom cultural technique fields, and in particular to a kind of Pleurotus eryngii culture medium and Pleurotus eryngii inoculation method.
Background technique
It on traditional planting almond abalone mushroom, not enough standardizes in the control of humidity and temperature, and is arranged without explicitly ventilation and heat preservation
It applies, and nutrient content is lower, larger for open cultivation, the wasting of resources, protein content is low, causes mouthfeel poor.
Summary of the invention
The purpose of the present invention is to provide a kind of Pleurotus eryngii culture medium and Pleurotus eryngii inoculation methods, to solve above-mentioned background skill
It on the traditional planting almond abalone mushroom proposed in art, is not enough standardized in the control of humidity and temperature, and without explicitly divulging information and keeping the temperature
Measure, and nutrient content is lower, larger for open cultivation, the wasting of resources, protein content is low, causes mouthfeel is poor to ask
Topic.
To achieve the above object, the invention provides the following technical scheme: a kind of Pleurotus eryngii culture medium and Pleurotus eryngii cultivation side
Method, including chitosan 6-8 parts by weight, diatomite 1.5-1.8 parts by weight, amino polyphosphazene microspheres 0.5-0.8 parts by weight, wheat straw
42-45 parts by weight, rice husk 30-32 parts by weight, dregs of beans 8-10.3 parts by weight.
Specific inoculation method is as follows:
Step 1: chitosan, diatomite, amino polyphosphazene microspheres, wheat straw, rice husk and dregs of beans are pulverized and mixed, and pass through sieve
Net carries out sieving, guarantees its density size.
Step 2: water is added in the compost after step 1 is mixed, and carries out 3-5 turning, and being formulated as pH value is 7-7.5's
Culture medium, moisture content has water to dissociate with finger grip, and does not drip as standard.
Step 3: the vinyon cylinder film that radius is 9-10cm is cut into the cylinder set of 55-60cm length, cylinder is covered into one end
It is fastened with cotton rope, the culture medium that step 2 is handled well is fitted into cylinder set, is fastened again with the other end that cotton rope covers cylinder after installing.
Step 4: the charged cylinder set of step 3 is subjected to sterilization treatment, impassioned cylinder set is sent into thermal barrel, with progressive
Mode rose to 100 DEG C at 3-5.5 hours, and temperature is held in 35-36 hours.
Step 5: the cylinder set disengaging inoculation processing after step 4 is handled equipped with culture medium, and the 1st to 7 day after inoculation
By it with humidity to grow in the environment of 65-72%, progress successively turning in the 8th day keeps room temperature to exist between the 8th to 15 day
22-26 DEG C, and ventilation 3-4 times daily, every time it is 45-55 minutes, carried out turning, the 16th to 20 day holding room again at the 16th day
Temperature is at 21-23 DEG C, and growth humidity environment is 58-68%, and ventilation 2-3 times, 70-120 minutes each daily, at the 21st day,
Grow mycelia takes bundle aperture, and the 21st to 29 day, room temperature was 20-22 DEG C, ambient humidity 57-65%, and lasting guarantor
Ventilation state is held, the 30th day, carries out pricking mesoporous processing again around hole tying, and carry out turning heat dissipation to it, to the 40th
It when, upper macropore is pricked around mesoporous, at the 50th to 60 day, when cylinder film surface has protrusion, cylinder film internal water accumulation is discharged.
Step 6: the cylinder set after the raw bacterium of step 5 is moved into greenhouse, and covers canopy film heat preservation, temperature of shed is adjusted to 17-
21 DEG C, humidity 90-95%, it is adjusted to 5-7 DEG C at a temperature of general after 9-10h, keeps 9-10h, completes first time flower bud to reach,
And 3-5 flower bud is carried out by this method, then it is 15-17 DEG C that temperature of shed, which is controlled, when mushroom diameter reaches 1-2cm, in film
Upper opening is removed canopy film to after 2.5-3cm convenient for Pleurotus eryngii to outgrowth Pleurotus eryngii is long so that Pleurotus eryngii further at
Type.
Further, in the step 5 at the 21st day, the radius for pricking hole is 0.03-0.05cm, and quantity is 3-5, and
Depth is 1-1.2cm, and the 30th day pore quantity is 6-7, and radius is 0.1-0.2cm, depth 2-2.2cm, and radius is
0.12-0.2.2cm。
Further, described that modified sheet silicate mineral powder is added in step 1, parts by weight are 1.5-2 weight
Part.
Further, the size of the step 1 sieve pore is 0.3-0.7cm.
Compared with prior art, the beneficial effects of the present invention are: culture cost of material is lower, technique is simpler, and it has
Machine object content is high, nutrition covering scope is big, keeps the Pleurotus eryngii turned out fertile, is rich in protein abundant, calcium, phosphorus and vitamin
Deng.
Specific embodiment
Below in conjunction with the embodiment of the present invention, technical scheme in the embodiment of the invention is clearly and completely described,
Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based in the present invention
Embodiment, every other embodiment obtained by those of ordinary skill in the art without making creative efforts, all
Belong to the scope of protection of the invention.
Embodiment 1
A kind of Pleurotus eryngii culture medium and Pleurotus eryngii inoculation method, including 6 parts by weight of chitosan, 1.5 parts by weight of diatomite, ammonia
0.5 parts by weight of base poly (organophosphazenes) microsphere, 42 parts by weight of wheat straw, 30 parts by weight of rice husk, 8 parts by weight of dregs of beans.
Specific inoculation method is as follows:
Step 1: chitosan, diatomite, amino polyphosphazene microspheres, wheat straw, rice husk and dregs of beans are pulverized and mixed, and pass through sieve
Net carries out sieving, guarantees its density size.
Step 2: water is added in the compost after step 1 is mixed, and carries out 3 turnings, is formulated as the culture that pH value is 7
Base, moisture content has water to dissociate with finger grip, and does not drip as standard.
Step 3: the vinyon cylinder film that radius is 9cm is cut into the cylinder set of 55cm length, cylinder is covered into one end cotton rope
It fastens, the culture medium that step 2 is handled well is fitted into cylinder set, is fastened again with the other end that cotton rope covers cylinder after installing.
Step 4: the charged cylinder set of step 3 is subjected to sterilization treatment, impassioned cylinder set is sent into thermal barrel, with progressive
Mode rose to 100 DEG C at 3 hours, and temperature is held in 35 hours.
Step 5: the cylinder set disengaging inoculation processing after step 4 is handled equipped with culture medium, and the 1st to 7 day after inoculation
By its with humidity be 65% in the environment of grow, progresss successively turning in the 8th day, between the 8th to 15 day, holding room temperature 22
DEG C, and daily ventilation 3 times, every time be 45 minutes, carried out turning again at the 16th day, the 16th to 20 day holding room temperature at 21 DEG C,
Growing humidity environment is 58%, at ventilation daily 2 times, every time 70 minutes, the 21st day, takes bundle aperture grown mycelia,
21st to 29 day, room temperature was 20 DEG C, ambient humidity 57%, and persistently keeps ventilation state, the 30th day, was tying hole
Around carry out pricking mesoporous processing again, and turning heat dissipation is carried out to it, when the 40th day, upper macropore is pricked around mesoporous, the
50 to 60 days, when cylinder film surface has protrusion, cylinder film internal water accumulation is discharged.
Step 6: the cylinder set after the raw bacterium of step 5 is moved into greenhouse, and covers canopy film heat preservation, temperature of shed is adjusted to 17
DEG C, humidity 90%, after 9h will at a temperature of be adjusted to 5 DEG C, keep 9h, complete first time flower bud to reach, and by this method into
3 flower buds of row, then it is 15 DEG C that temperature of shed, which is controlled, when mushroom diameter reaches 1cm, in film upper opening, is convenient for Pleurotus eryngii pair
Outgrowth is removed canopy film, after Pleurotus eryngii length to 2.5cm so that Pleurotus eryngii further forms.
Wherein, in the step 5 at the 21st day, the radius for pricking hole is 0.03cm, and quantity is 3, and depth is 1cm, the
30 days pore quantities are 6, and radius is 0.1cm, depth 2cm, radius 0.12cm.
Wherein, described that modified sheet silicate mineral powder is added in step 1, parts by weight are 1.5 parts by weight.
Wherein, the size of the step 1 sieve pore is 0.3cm.
Embodiment 2
A kind of Pleurotus eryngii culture medium and Pleurotus eryngii inoculation method, including 8 parts by weight of chitosan, 1.8 parts by weight of diatomite, ammonia
0.8 parts by weight of base poly (organophosphazenes) microsphere, 45 parts by weight of wheat straw, 32 parts by weight of rice husk, 10.3 parts by weight of dregs of beans.
Specific inoculation method is as follows:
Step 1: chitosan, diatomite, amino polyphosphazene microspheres, wheat straw, rice husk and dregs of beans are pulverized and mixed, and pass through sieve
Net carries out sieving, guarantees its density size.
Step 2: water is added in the compost after step 1 is mixed, and carries out 5 turnings, is formulated as the culture that pH value is 7.5
Base, moisture content has water to dissociate with finger grip, and does not drip as standard.
Step 3: the vinyon cylinder film that radius is 10cm is cut into the cylinder set of 60cm length, cylinder is covered into one end line
Rope is fastened, and the culture medium that step 2 is handled well is fitted into cylinder set, is fastened again with the other end that cotton rope covers cylinder after installing.
Step 4: the charged cylinder set of step 3 is subjected to sterilization treatment, impassioned cylinder set is sent into thermal barrel, with progressive
Mode rose to 100 DEG C at 5.5 hours, and temperature is held in 36 hours.
Step 5: the cylinder set disengaging inoculation processing after step 4 is handled equipped with culture medium, and the 1st to 7 day after inoculation
By its with humidity be 72% in the environment of grow, progresss successively turning in the 8th day, between the 8th to 15 day, holding room temperature 26
DEG C, and daily ventilation 4 times, every time be 55 minutes, carried out turning again at the 16th day, the 16th to 20 day holding room temperature at 23 DEG C,
Growing humidity environment is 68%, at ventilation daily 3 times, every time 120 minutes, the 21st day, takes bundle aperture grown mycelia,
21st to 29 day, room temperature was 22 DEG C, ambient humidity 65%, and persistently keeps ventilation state, the 30th day, was tying hole
Around carry out pricking mesoporous processing again, and turning heat dissipation is carried out to it, when the 40th day, upper macropore is pricked around mesoporous, the
50 to 60 days, when cylinder film surface has protrusion, cylinder film internal water accumulation is discharged.
Step 6: the cylinder set after the raw bacterium of step 5 is moved into greenhouse, and covers canopy film heat preservation, temperature of shed is adjusted to 21
DEG C, humidity 95% is adjusted to 7 DEG C at a temperature of general after 10h, keep 10h, completes first time flower bud to reach, and by this method
5 flower buds are carried out, then it is 17 DEG C that temperature of shed, which is controlled, when mushroom diameter reaches 2cm, in film upper opening, is convenient for Pleurotus eryngii
Outgrowth is removed canopy film, after Pleurotus eryngii length to 3cm so that Pleurotus eryngii further forms.
Wherein, in the step 5 at the 21st day, the radius for pricking hole is 0.05cm, and quantity is 5, and depth is 1.2cm,
30th day pore quantity is 7, and radius is 0.2cm, depth 2.2cm, radius 0.2.2cm.
Wherein, described that modified sheet silicate mineral powder is added in step 1, parts by weight are 2 parts by weight.
Wherein, the size of the step 1 sieve pore is 0.7cm.
The working principle of the invention: culture cost of material is lower, and technique is simpler, and its high organic content, nutrition are contained
Lid range is big, keeps the Pleurotus eryngii turned out fertile, rich in protein abundant, calcium, phosphorus and vitamin etc..
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (4)
1. a kind of Pleurotus eryngii culture medium and Pleurotus eryngii inoculation method, which is characterized in that following parts by weight material: chitosan 6-8 weight
Measure part, diatomite 1.5-1.8 parts by weight, amino polyphosphazene microspheres 0.5-0.8 parts by weight, wheat straw 42-45 parts by weight, rice husk 30-
32 parts by weight, dregs of beans 8-10.3 parts by weight.
Specific inoculation method is as follows:
Step 1: chitosan, diatomite, amino polyphosphazene microspheres, wheat straw, rice husk and dregs of beans are pulverized and mixed, by sieve into
Row sieving guarantees its density size.
Step 2: water is added in the compost after step 1 is mixed, and carries out 3-5 turning, is formulated as the culture that pH value is 7-7.5
Base, moisture content has water to dissociate with finger grip, and does not drip as standard.
Step 3: the vinyon cylinder film that radius is 9-10cm is cut into the cylinder set of 55-60cm length, cylinder is covered into one end line
Rope is fastened, and the culture medium that step 2 is handled well is fitted into cylinder set, is fastened again with the other end that cotton rope covers cylinder after installing.
Step 4: the charged cylinder set of step 3 is subjected to sterilization treatment, impassioned cylinder set is sent into thermal barrel, in progressive mode
100 DEG C were risen at 3-5.5 hours, and temperature is held in 35-36 hours.
Step 5: after step 4 is handled equipped with culture medium cylinder set disengaging inoculation processing, and be inoculated with after the 1st to 7 day by its
It is grown in the environment of being 65-72% with humidity, progress successively turning in the 8th day keeps room temperature in 22-26 between the 8th to 15 day
DEG C, and ventilation 3-4 times daily, every time it is 45-55 minutes, carried out turning again at the 16th day, holding room temperature exists within the 16th to 20 day
21-23 DEG C, growth humidity environment is 58-68%, and ventilation 2-3 times, 70-120 minutes each daily, at the 21st day, is being grown
Mycelia takes bundle aperture, and the 21st to 29 day, room temperature was 20-22 DEG C, ambient humidity 57-65%, and lasting holding is logical
Wind state the 30th day, carries out pricking mesoporous processing around hole, and carry out turning heat dissipation to it again tying, when the 40th day,
Upper macropore is pricked around mesoporous, and at the 50th to 60 day, when cylinder film surface has protrusion, cylinder film internal water accumulation is discharged.
Step 6: the cylinder set after the raw bacterium of step 5 being moved into greenhouse, and covers canopy film heat preservation, and temperature of shed is adjusted to 17-21 DEG C,
Humidity is 90-95%, and 5-7 DEG C is adjusted at a temperature of general after 9-10h, keeps 9-10h, completes first time flower bud to reach, and with
This mode carries out 3-5 flower bud, then it is 15-17 DEG C that temperature of shed, which is controlled, when mushroom diameter reaches 1-2cm, opens on film
Mouthful, outgrowth is removed canopy film, after Pleurotus eryngii length to 2.5-3cm so that Pleurotus eryngii further forms convenient for Pleurotus eryngii.
2. a kind of Pleurotus eryngii culture medium according to claim 1 and Pleurotus eryngii inoculation method, it is characterised in that: the step
In five at the 21st day, the radius for pricking hole is 0.03-0.05cm, and quantity is 3-5, and depth is 1-1.2cm, the 30th day hole
Quantity is 6-7, and radius is 0.1-0.2cm, depth 2-2.2cm, radius 0.12-0.2.2cm.
3. a kind of Pleurotus eryngii culture medium according to claim 1 and Pleurotus eryngii inoculation method, it is characterised in that: described in step
Modified sheet silicate mineral powder is added in rapid one, parts by weight are 1.5-2 parts by weight.
4. a kind of Pleurotus eryngii culture medium according to claim 1 and Pleurotus eryngii inoculation method, it is characterised in that: the step
The size of one sieve pore is 0.3-0.7cm.
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| CN201811073553.4A CN109197383A (en) | 2018-09-14 | 2018-09-14 | A kind of Pleurotus eryngii culture medium and Pleurotus eryngii inoculation method |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103922839A (en) * | 2014-04-11 | 2014-07-16 | 芜湖野树林生物科技有限公司 | Rice straw culture medium and preparation method thereof |
| CN107691111A (en) * | 2017-10-13 | 2018-02-16 | 大连森源菌业有限公司 | A kind of method for planting almond abalone mushroom |
| CN107969286A (en) * | 2017-12-15 | 2018-05-01 | 马行健 | A kind of method and culture medium for cultivating flower mushroom |
| CN108293601A (en) * | 2016-09-21 | 2018-07-20 | 绿美食用菌科技发展江苏有限公司 | A kind of Pleurotus eryngii culture medium and Pleurotus eryngii inoculation method |
-
2018
- 2018-09-14 CN CN201811073553.4A patent/CN109197383A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103922839A (en) * | 2014-04-11 | 2014-07-16 | 芜湖野树林生物科技有限公司 | Rice straw culture medium and preparation method thereof |
| CN108293601A (en) * | 2016-09-21 | 2018-07-20 | 绿美食用菌科技发展江苏有限公司 | A kind of Pleurotus eryngii culture medium and Pleurotus eryngii inoculation method |
| CN107691111A (en) * | 2017-10-13 | 2018-02-16 | 大连森源菌业有限公司 | A kind of method for planting almond abalone mushroom |
| CN107969286A (en) * | 2017-12-15 | 2018-05-01 | 马行健 | A kind of method and culture medium for cultivating flower mushroom |
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Application publication date: 20190115 |