CN109170663A - Pyracantha fortuneana fruit powder and preparation method thereof with anticancer function - Google Patents
Pyracantha fortuneana fruit powder and preparation method thereof with anticancer function Download PDFInfo
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- CN109170663A CN109170663A CN201810988806.4A CN201810988806A CN109170663A CN 109170663 A CN109170663 A CN 109170663A CN 201810988806 A CN201810988806 A CN 201810988806A CN 109170663 A CN109170663 A CN 109170663A
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- pyracantha fortuneana
- fruit
- pyracantha
- preparation
- anticancer function
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- 238000002360 preparation method Methods 0.000 title claims abstract description 38
- 230000007071 enzymatic hydrolysis Effects 0.000 claims abstract description 18
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims abstract description 18
- 238000001914 filtration Methods 0.000 claims abstract description 13
- 239000007788 liquid Substances 0.000 claims description 68
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 66
- 238000001035 drying Methods 0.000 claims description 59
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 46
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 7
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- 238000010257 thawing Methods 0.000 claims description 7
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 6
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- CIWBSHSKHKDKBQ-DUZGATOHSA-N D-isoascorbic acid Chemical compound OC[C@@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-DUZGATOHSA-N 0.000 description 1
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- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
- A23L19/01—Instant products; Powders; Flakes; Granules
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/40—Colouring or decolouring of foods
- A23L5/41—Retaining or modifying natural colour by use of additives, e.g. optical brighteners
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicinal Preparation (AREA)
Abstract
The pyracantha fortuneana fruit powder and preparation method thereof with anticancer function that the invention discloses a kind of.The preparation method of the pyracantha fortuneana fruit powder with anticancer function is the following steps are included: I, is pre-processed；II, enzymatic hydrolysis；III, filtering；IV, is dry.The present invention has the powdery of pyracantha fortuneana fruit powder of anticancer function, color, in good taste, full of nutrition, has many advantages, such as good anticancer function, can be applied in the products such as health food, beverage, bread product, greatly improves the economic benefit of pyracantha fortuneana fruit.
Description
Technical field
The present invention relates to food processing technology fields, and in particular to pyracantha fortuneana fruit powder and its preparation side with anticancer function
Method.
Background technique
Pyracantha fortuneana fruit is resourceful, the south Chinas mountain area such as main product Yu Chuan, Yunnan, Guizhou Province, Shan, Hubei Province, Hunan and knob, Sichuan and
Two province's fresh fruit annual output of Guizhou is at 2.5 ten thousand tons or more, and Hubei South-West Hubei and Shennongjiawooded Area produce nearly 50,000 tons of fruit every year, Hunan
Only Xiangxi Autonomous Prefecture pyracantha fortuneana fresh fruit annual output is just at 1.1 ten thousand tons or more.Pyracantha fortuneana fruit is the ripening fruits of rosaceous plant pyracantha fortuneana, custom
Claim torch fruit, red lantern, spine also known as rescues army provisions, water branch.Rosaceae, evergreen shrubs, be it is a kind of can the edible ornamental of medicine it is more
Purposes flowers and fruits plant is used as medicine with fruit, root, leaf, mild-natured, sweet in flavor, sour, and leaf can be clearing heat and detoxicating, and sore swollen toxin is controlled in external application, is a kind of
Fabulous spring sees flower, winter fruit effect plant.Amino acid rich in fruit, vitamin, fatty acid, soluble polysaccharide,
The nutritional ingredients such as pectin and dietary fiber, especially it is worth mentioning that flavones also rich in, polyphenol, red in firethorn fruit
Element and uranidin and canopy skin element have reducing blood lipid, improve immunity, is antifatigue, removing free radical, anti-oxidant etc., wide
General many fields applied to national economy such as food, medicine, light industry, space flight, agriculture and animal husbandry fisheries.
It is few to the development and utilization of pyracantha fortuneana fruit resource at present, it is also seldom to the exploitation of its processed side product, it is only limitted to production fire
Spine fruit oil extracts flavones, and seed peel and pericarp make feed, and a large amount of fruit juice has a large amount of pyracantha fortuneanas in addition to minority is utilized every year
Fruit goes bad and is wasted due to fresh-keeping improper.Pyracantha fortuneana fruit can be processed to the products such as fruit wine, fruit vinegar, fruit juice.Wherein, it utilizes
Pyracantha fortuneana fruit production can be applied in the products such as beverage, bread product, health food rich in the fruit powder of nutritional ingredient, improve product battalion
Quality is supported, while widening pyracantha fortuneana fruit products application range, greatly improves pyracantha fortuneana fruit added value, and be readily transported and store, is extended
Shelf life.The drying mode that fruit powder processing enterprise generally uses mainly has spray drying, freeze-drying, heated-air drying, true
Sky drying etc., temperature of charge is higher when being spray-dried powder processed, and nutritional ingredient, color and the flavor of product are more easily damaged, seriously
When even generate be charred taste.Though freeze-drying can preferably keep the nutriment in fruit and vegetable materials, energy consumption height, production cost
Its industrialization promotion is limited greatly.The fruit vegetable powder nutrition crushed after heated-air drying and functional component lose more, product wind
Taste is poor, and color is undesirable.Vacuum drying drying time is long, and drying efficiency is low, is not suitable for industrialization production.
For the utilizing status for improving pyracantha fortuneana, extends pyracantha fortuneana selling time, widen pyracantha fortuneana product category, promote the economy of pyracantha fortuneana
Value, it is necessary to further to the deep processing of pyracantha fortuneana to be studied.
Summary of the invention
In view of the above-mentioned deficiencies in the prior art, the pyracantha fortuneana fruit powder that the present invention provides a kind of with anticancer function and
Preparation method.
A kind of preparation method of the pyracantha fortuneana fruit powder with anticancer function, comprising the following steps:
I, pretreatment: ripe very likely, no disease and pests harm fresh firethorn fruit, the fire after being cleaned after cleaning up are chosen
Spine fruit；First the pyracantha fortuneana fruit after cleaning is placed at 2-6 DEG C and carries out break process, is subsequently placed into rubber mill and carries out glue mill, obtain
Pyracantha fortuneana pulp；
II, enzymatic hydrolysis: to above-mentioned pyracantha fortuneana fruit slurry in be added protective agent, sodium chloride, citric acid, the pyracantha fortuneana pulp, protective agent,
Sodium chloride, citric acid mass ratio be 100:(0.03-0.08): (0.01-0.04): (0.02-0.05), mix, obtain pyracantha fortuneana
Colour protecting liquid；Then be added complex enzyme into pyracantha fortuneana colour protecting liquid, the pyracantha fortuneana colour protecting liquid, complex enzyme mass ratio be 50:(0.02-
0.06) 1.5-3.5h, is digested at 30-45 DEG C, obtains pyracantha fortuneana enzymolysis liquid；
III, filtering: being filtered processing to above-mentioned pyracantha fortuneana enzymolysis liquid, remove the residue in pulp, obtain pyracantha fortuneana filtered fluid,
It is subsequently added into the compound drying aid of pyracantha fortuneana filtered fluid quality 3-9%, pyracantha fortuneana mixed liquor is obtained after mixing；
IV, is dry: carrying out spray drying treatment to above-mentioned pyracantha fortuneana mixed liquor, drying to water content is 2-6wt%, is had
There is the pyracantha fortuneana fruit powder of anticancer function.
Preferably, the preparation method of the pyracantha fortuneana fruit powder with anticancer function, comprising the following steps:
I, pretreatment: ripe very likely, no disease and pests harm fresh firethorn fruit, the fire after being cleaned after cleaning up are chosen
Spine fruit；First the pyracantha fortuneana fruit after cleaning is placed at 0-4 DEG C, 2-5h is pre-chilled, is then placed in subzero 120 DEG C-subzero 20 DEG C of liquid nitrogen
Under freeze 10-25min, the fruit freezed be put at subzero 5 DEG C of subzero 15- refrigerate 12-60h after, be placed at 2-6 DEG C and solved
Freeze, the pyracantha fortuneana fruit after being thawed；Pyracantha fortuneana fruit after above-mentioned defrosting is placed at 2-6 DEG C and carries out break process, is subsequently placed into glue
Glue mill is carried out in grinding machine, obtains pyracantha fortuneana pulp；
II, enzymatic hydrolysis: to above-mentioned pyracantha fortuneana fruit slurry in be added protective agent, sodium chloride, citric acid, the pyracantha fortuneana pulp, protective agent,
Sodium chloride, citric acid mass ratio be 100:(0.03-0.08): (0.01-0.04): (0.02-0.05), mix, obtain pyracantha fortuneana
Colour protecting liquid；Then be added complex enzyme into pyracantha fortuneana colour protecting liquid, the pyracantha fortuneana colour protecting liquid, complex enzyme mass ratio be 50:(0.02-
0.06) 1.5-3.5h, is digested at 30-45 DEG C, obtains pyracantha fortuneana enzymolysis liquid；
III, filtering: being filtered processing to above-mentioned pyracantha fortuneana enzymolysis liquid, remove the residue in pulp, obtain pyracantha fortuneana filtered fluid,
It is subsequently added into the compound drying aid of pyracantha fortuneana filtered fluid quality 3-9%, pyracantha fortuneana mixed liquor is obtained after mixing；
IV, is dry: carrying out spray drying treatment to above-mentioned pyracantha fortuneana mixed liquor, drying to water content is 2-6wt%, is had
There is the pyracantha fortuneana fruit powder of anticancer function.
It is highly preferred that the preparation method of the pyracantha fortuneana fruit powder with anticancer function, comprising the following steps:
I, pretreatment: ripe very likely, no disease and pests harm fresh firethorn fruit, the fire after being cleaned after cleaning up are chosen
Spine fruit；First the pyracantha fortuneana fruit after cleaning is placed at 0-4 DEG C, 2-5h is pre-chilled, is then placed in subzero 120 DEG C-subzero 20 DEG C of liquid nitrogen
Under freeze 10-25min, the fruit freezed be put at subzero 5 DEG C of subzero 15- refrigerate 12-60h after, be placed at 2-6 DEG C and solved
Freeze, the pyracantha fortuneana fruit after being thawed；Pyracantha fortuneana fruit after above-mentioned defrosting is placed at 2-6 DEG C and carries out break process, is subsequently placed into glue
Glue mill is carried out in grinding machine, obtains pyracantha fortuneana pulp；
II, enzymatic hydrolysis: being added protective agent, sodium chloride, citric acid into above-mentioned pyracantha fortuneana fruit slurry,
The pyracantha fortuneana pulp, protective agent, sodium chloride, citric acid mass ratio be 100:
(0.03-0.08): (0.01-0.04): (0.02-0.05) mixes, obtains pyracantha fortuneana colour protecting liquid；Then it is protected to pyracantha fortuneana
Be added complex enzyme in color liquid, the pyracantha fortuneana colour protecting liquid, complex enzyme mass ratio be 50:(0.02-0.06), the enzyme at 30-45 DEG C
1.5-3.5h is solved, pyracantha fortuneana enzymolysis liquid is obtained；
III, filtering: being filtered processing to above-mentioned pyracantha fortuneana enzymolysis liquid, remove the residue in pulp, obtain pyracantha fortuneana filtered fluid,
It is subsequently added into the compound drying aid of pyracantha fortuneana filtered fluid quality 3-9%, pyracantha fortuneana mixed liquor is obtained after mixing；
IV, is dry: first carrying out vacuum freeze drying processing to pyracantha fortuneana mixed liquor, drying to water content is 20-40wt%, is obtained
To the pyracantha fortuneana powder of primary drying；Then spray drying treatment is carried out to the pyracantha fortuneana powder of gained primary drying, drying to water content is
2-6wt% obtains the pyracantha fortuneana fruit powder with anticancer function.
Preferably, the inlet air temperature of the spray drying and leaving air temp are controlled respectively at 145 DEG C -165 DEG C, 65-75 DEG C.
The protective agent includes sodium citrate, L-cysteine hydrochloride, one in Ascorbic acid chitosan quaternary ammonium salt
Kind is a variety of.
Preferably, the protective agent is pressed by sodium citrate, L-cysteine hydrochloride, Ascorbic acid chitosan quaternary ammonium salt
(1-5): (1-5): the mass ratio of (1-5) mixes.
The complex enzyme includes pectase, cellulase, hemicellulase, two or more in zytase.
The compound drying aid include lactalbumin, beta-cyclodextrin, modified beta-cyclodextrin, two kinds in soluble starch or
It is a variety of.
Preferably, the compound drying aid presses (1-5) by lactalbumin, beta-cyclodextrin, soluble starch: (1-5): (1-
5) mass ratio mixes.
It is highly preferred that the compound drying aid presses (1-5) by lactalbumin, modified beta-cyclodextrin, soluble starch: (1-
5): the mass ratio of (1-5) mixes.
The modified beta-cyclodextrin the preparation method comprises the following steps: weighing that 2-5 parts of beta-cyclodextrins, 0.3-0.7 parts along fourths by mass parts
Enedioic acid, 3-10 part sodium dihydric hypophosphite, 0.01-0.03 parts of adjacent methyl hydroquinones, mixing obtain mixture；By mass body
Product is than 1:(2-4) (g/mL) addition 40wt%-60wt% ethanol water into mixture, it mixes and is placed on 100 DEG C -120 DEG C
Under carry out reaction 2-6h, obtain reactant after cooling, then by 1:(3-5) nothing is added into reactant the mass volume ratio of (g/mL)
Water-ethanol is stood, and is filtered, and is modified beta-cyclodextrin to obtain the final product after dry.
The pyracantha fortuneana fruit powder with anticancer function, using the preparation of the pyracantha fortuneana fruit powder of the present invention with anticancer function
Method is prepared.
By adopting the above-described technical solution, compared with prior art, the present invention has the following advantages: the present invention has
There is the pyracantha fortuneana fruit powder of anticancer function to joined compound drying aid before it is dried, and combine suitable drying process, fruit can be improved
The integrated quality of powder can keep fruit powder to have good character, color etc.；In the pyracantha fortuneana fruit of the present invention with anticancer function
In the preparation method of powder, it is added to protective agent, effectively reduces the loss of firethorn fruit Central Plains active constituent；In addition.The present invention
Middle pyracantha fortuneana fruit reduces the nutrients in fruit powder preparation process by first freezing again the processing of the methods of natural thaw, stage drying
The loss of matter and flavor component obtains the pyracantha fortuneana fruit powder of high level nutritional ingredient.The present invention has the pyracantha fortuneana of anticancer function
It is the powdery of fruit powder, color, in good taste, it is full of nutrition, there is good anticancer function etc., can be applied to health food, beverage, face
It packs in the products such as product, greatly improves the economic benefit of pyracantha fortuneana.
Specific embodiment
Foregoing invention content of the invention is described in further detail With reference to embodiment, but should not be incited somebody to action
This range for being interpreted as the above-mentioned theme of the present invention is only limitted to following embodiments.
Each raw material and equipment introduction in embodiment:
Pyracantha fortuneana fruit originates from the Wild Fruit of Pyracantha in Chongqing.
Pectase, No. CAS: 9032-75-1, enzyme activity 10000U/g are limited purchased from Shandong Su Kehan bioengineering share
Company.
Cellulase, No. CAS: 9042-54-8, enzyme activity 20000U/g have purchased from Shandong Su Kehan bioengineering share
Limit company.
Sodium citrate, No. CAS: 6132-04-3,60 mesh of granularity, food-grade is purchased from Shandong Lemon Biochemical Co., Ltd..
L-cysteine hydrochloride, No. CAS: 52-89-1,60 mesh of granularity, food-grade far has at creating science and technology purchased from Wuhan
Limit company.
Chitosan, No. CAS: 9012-76-4,80 mesh of granularity, food-grade, purchased from Wuhan far at creating Science and Technology Ltd..
N-Methyl pyrrolidone, No. CAS: 872-50-4, top grade product are purchased from BASF Aktiengesellschaft.
Iodomethane, No. CAS: 74-88-4, SILVER REAGENT is purchased from Nanjing Huang Tai Pharmaceutical Technology Co., Ltd.
Acetone, No. CAS: 67-64-1, SILVER REAGENT is purchased from Shanghai De-Nol Chemical Co., Ltd..
Sodium isoascorbate, No. CAS: 6381-77-7, food-grade is auspicious at Biotechnology Co., Ltd purchased from Henan.
Ascorbic acid chitosan quaternary ammonium salt the preparation method comprises the following steps:
Chitosan is added in N-Methyl pyrrolidone I, stirs 10h at 30 DEG C with the revolving speed of 350r/min, and iodine is added
Change sodium, 1mol/L sodium hydrate aqueous solution, iodomethane, keeps revolving speed constant, the reaction was continued at 55 DEG C for 24 hours, the chitosan, N-
Methyl pyrrolidone, sodium iodide, sodium hydrate aqueous solution, iodomethane mass ratio be 1:50:2:8:11, chitosan is then added
The acetone that 155 times of quality is precipitated, and is filtered, and gained filter cake is placed at 50 DEG C dry 50h, obtains N, N, N- trimethyl chitin
Quaternary ammonium salt；
II, presses the mass volume ratio of 1:18 (g/ml), and to above-mentioned N, N is added in N- trimethyl chitin quaternary ammonium salt
18wt% arabo-ascorbic acid sodium water solution, stirs 20min under the conditions of 30 DEG C, 400r/min, and gained mixed liquor pours into bag filter
In, the port of bag filter is pricked extremely, then the bag filter equipped with liquid is put into distilled water, entire bag filter is totally immersed into
In distilled water, dialyse 60h at 4 DEG C, and every 6h changes primary distilled water, and acquired solution carries out vacuum freeze drying, and vacuum refrigeration is dry
Dry condition is control material thickness 8mm, sets pre-freezing temperature as -20 DEG C, keeps 1.5h after sample temperature drops to -20 DEG C,
Sublimation temperature is set as 12 DEG C, resolution temperature is 34 DEG C, vacuum degree 25pa, dry for 24 hours up to Ascorbic acid chitosan quaternary ammonium
Salt.
Lactalbumin, referring to application No. is preparation methods shown in embodiment 1 in the Chinese patent of 201410383063.X to obtain
It arrives.
Beta-cyclodextrin, No. CAS: 7585-39-9, food-grade purchases hundred taste Biotechnology Co., Ltd of Hebei.
Maleic acid, No. CAS: 110-16-7, it is purchased from Aladdin reagent Co., Ltd.
Sodium dihydric hypophosphite, No. CAS: 7681-53-0, it is purchased from Sinopharm Chemical Reagent Co., Ltd..
Adjacent methyl hydroquinone, No. CAS: 95-71-6, it is purchased from Sinopharm Chemical Reagent Co., Ltd..
Soluble starch, No. CAS: 9005-84-9,80 mesh of granularity, food-grade pleases the limited public affairs of chemical products purchased from Henan China
Department.
Pulverizer is MCF type Universalpulverizer, is purchased from Jiangyin City Jia Ke Machinery Manufacturing Co., Ltd.
It is JMF-80 type rubber mill that glue, which grinds equipment, is purchased from Guangzhou Heng Dong mechanical equipment Science and Technology Ltd..
Mixing plant is FJ200 type high speed dispersion homogenizer, is purchased from Shanghai Da Ping Instrument Ltd..
Water bath chader is XB-2A type water-bath constant temperature oscillator, is purchased from Jintan area White Tower Xin Bao instrument plant.
Bag filter, diameter 36mm, molecular cut off 14kDa win Science and Technology Ltd. purchased from hundred Aurion of Beijing.
400 mesh shake the model XFC800-1s of screening machine, are purchased from Xinxiang City pioneer vibrating machine Co., Ltd.
Vacuum freeze is LGJ-30D type vacuum freeze drier, is purchased from the limited public affairs of promise biotechnology as Shanghai
Department.
Spray drying device is ZOLLO-6000Y type spray dryer, is purchased from Shanghai Zuo Le Instrument Ltd..
Embodiment 1
A kind of preparation method of the pyracantha fortuneana fruit powder with anticancer function, comprising the following steps:
I, pretreatment: ripe very likely, no disease and pests harm fresh firethorn fruit, the fire after being cleaned after cleaning up are chosen
Spine fruit；Pyracantha fortuneana fruit after cleaning is placed in 3 DEG C, is crushed 10min in the pulverizer of 4500r/min, is subsequently placed into 10 DEG C, 1800r/
Glue mill is carried out in the rubber mill of min obtains pyracantha fortuneana pulp after glue grinds 20min；
II, enzymatic hydrolysis: sodium citrate, L-cysteine hydrochloride, Ascorbic acid shell are added into above-mentioned pyracantha fortuneana fruit slurry and gathers
Sugared quaternary ammonium salt, sodium chloride, citric acid, the pyracantha fortuneana pulp, sodium citrate, L-cysteine hydrochloride, Ascorbic acid shell are poly-
Sugared quaternary ammonium salt, sodium chloride, citric acid mass ratio be 100:0.01:0.01:0.01:0.02:0.02, with 400r/ at 25 DEG C
The revolving speed of min stirs 15min, obtains pyracantha fortuneana colour protecting liquid；Then pectase, cellulase are added into pyracantha fortuneana colour protecting liquid, it is described
Pyracantha fortuneana colour protecting liquid, pectase, cellulase mass ratio be 50:0.02:0.02, be put into 40 DEG C, the water-bath of 100r/min oscillation
2h is digested in device, obtains pyracantha fortuneana enzymolysis liquid；
III, filtering: processing is filtered to above-mentioned pyracantha fortuneana enzymolysis liquid using 100 mesh filter clothes, the residue in pulp is removed, obtains
To pyracantha fortuneana filtered fluid, it is subsequently added into the compound drying aid of pyracantha fortuneana filtered fluid quality 4.5%, with the revolving speed of 400r/min at 25 DEG C
25min is stirred, pyracantha fortuneana mixed liquor is obtained；
IV, is dry: carrying out spray drying treatment, the inlet air temperature and outlet air of the spray drying to above-mentioned pyracantha fortuneana mixed liquor
Temperature is respectively 150 DEG C, 70 DEG C, and hot wind wind speed is 4m3/ min, feeding speed 35mL/min, drying to water content are
4wt% obtains the pyracantha fortuneana fruit powder with anticancer function.
The compound drying aid is mixed by lactalbumin, beta-cyclodextrin, soluble starch 1:1:1 in mass ratio.
Embodiment 2
Substantially the same manner as Example 1, difference is only in that: the compound drying aid is by lactalbumin, beta-cyclodextrin by matter
Amount is mixed than 1:1.
Embodiment 3
Substantially the same manner as Example 1, difference is only in that: the compound drying aid is pressed by beta-cyclodextrin, soluble starch
Mass ratio 1:1 is mixed.
Embodiment 4
Substantially the same manner as Example 1, difference is only in that: the compound drying aid is pressed by lactalbumin, soluble starch
Mass ratio 1:1 is mixed.
Embodiment 5
Substantially the same manner as Example 1, difference is only in that: the compound drying aid by lactalbumin, modified beta-cyclodextrin,
Soluble starch 1:1:1 in mass ratio is mixed.
The modified beta-cyclodextrin the preparation method comprises the following steps: by mass parts weigh 4 parts of beta-cyclodextrins, 0.5 part of maleic acid,
6 parts of sodium dihydric hypophosphites, 0.01 part of adjacent methyl hydroquinone, stir 25min at 25 DEG C with the revolving speed of 400r/min, are mixed
Close material；Then 40wt% ethanol water is added into mixture by mass volume ratio 1:3 (g/mL), with 400r/ at 25 DEG C
The revolving speed of min stirs 25min, then is placed at 100 DEG C and reacts 3h, is cooled to 25 DEG C, obtains reactant；By the matter of 1:3 (g/mL)
Dehydrated alcohol is added into reactant for amount volume ratio, stands for 24 hours, filters, and washing filter cake repeatedly with dehydrated alcohol to pH is 7, connects
Filter cake is placed at 55 DEG C dry 14h to get modified beta-cyclodextrin.
Embodiment 6
A kind of preparation method of the pyracantha fortuneana fruit powder with anticancer function, comprising the following steps:
I, pretreatment: ripe very likely, no disease and pests harm fresh firethorn fruit, the fire after being cleaned after cleaning up are chosen
Spine fruit；Pyracantha fortuneana fruit after cleaning is placed in 3 DEG C, is crushed 10min in the pulverizer of 4500r/min, is subsequently placed into 10 DEG C, 1800r/
Glue mill is carried out in the rubber mill of min obtains pyracantha fortuneana pulp after glue grinds 20min；
II, enzymatic hydrolysis: being added sodium citrate, L-cysteine hydrochloride, sodium chloride, citric acid into above-mentioned pyracantha fortuneana fruit slurry,
The pyracantha fortuneana pulp, sodium citrate, L-cysteine hydrochloride, sodium chloride, citric acid mass ratio be 100:0.015:
0.015:0.02:0.02 stirs 15min at 25 DEG C with the revolving speed of 400r/min, obtains pyracantha fortuneana colour protecting liquid；Then it is protected to pyracantha fortuneana
In color liquid be added pectase, cellulase, the pyracantha fortuneana colour protecting liquid, pectase, cellulase mass ratio be 50:0.02:
0.02, it is put into the interior enzymatic hydrolysis 2h of water bath chader of 40 DEG C, 100r/min, obtains pyracantha fortuneana enzymolysis liquid；
III, filtering: processing is filtered to above-mentioned pyracantha fortuneana enzymolysis liquid using 100 mesh filter clothes, the residue in pulp is removed, obtains
To pyracantha fortuneana filtered fluid, it is subsequently added into the compound drying aid of pyracantha fortuneana filtered fluid quality 4.5%, with the revolving speed of 400r/min at 25 DEG C
25min is stirred, pyracantha fortuneana mixed liquor is obtained；
IV, is dry: carrying out spray drying treatment, the inlet air temperature and outlet air of the spray drying to above-mentioned pyracantha fortuneana mixed liquor
Temperature is respectively 150 DEG C, 70 DEG C, and hot wind wind speed is 4m3/ min, feeding speed 35mL/min, drying to water content are
4wt% obtains the pyracantha fortuneana fruit powder with anticancer function.
The compound drying aid is mixed by lactalbumin, beta-cyclodextrin, soluble starch 1:1:1 in mass ratio.
Embodiment 7
A kind of preparation method of the pyracantha fortuneana fruit powder with anticancer function, comprising the following steps:
I, pretreatment: ripe very likely, no disease and pests harm fresh firethorn fruit, the fire after being cleaned after cleaning up are chosen
Spine fruit；Pyracantha fortuneana fruit after cleaning is placed in 3 DEG C, is crushed 10min in the pulverizer of 4500r/min, is subsequently placed into 10 DEG C, 1800r/
Glue mill is carried out in the rubber mill of min obtains pyracantha fortuneana pulp after glue grinds 20min；
II, enzymatic hydrolysis: to above-mentioned pyracantha fortuneana fruit slurry in be added L-cysteine hydrochloride, Ascorbic acid chitosan quaternary ammonium salt,
Sodium chloride, citric acid, the pyracantha fortuneana pulp, L-cysteine hydrochloride, Ascorbic acid chitosan quaternary ammonium salt, sodium chloride, lemon
The mass ratio of lemon acid is 100:0.015:0.015:0.02:0.02, stirs 15min at 25 DEG C with the revolving speed of 400r/min, obtains
To pyracantha fortuneana colour protecting liquid；Then pectase, cellulase, the pyracantha fortuneana colour protecting liquid, pectase, fiber are added into pyracantha fortuneana colour protecting liquid
The mass ratio of plain enzyme is 50:0.02:0.02, is put into the interior enzymatic hydrolysis 2h of water bath chader of 40 DEG C, 100r/min, obtains pyracantha fortuneana enzymatic hydrolysis
Liquid；
III, filtering: processing is filtered to above-mentioned pyracantha fortuneana enzymolysis liquid using 100 mesh filter clothes, the residue in pulp is removed, obtains
To pyracantha fortuneana filtered fluid, it is subsequently added into the compound drying aid of pyracantha fortuneana filtered fluid quality 4.5%, with the revolving speed of 400r/min at 25 DEG C
25min is stirred, pyracantha fortuneana mixed liquor is obtained；
IV, is dry: carrying out spray drying treatment, the inlet air temperature and outlet air of the spray drying to above-mentioned pyracantha fortuneana mixed liquor
Temperature is respectively 150 DEG C, 70 DEG C, and hot wind wind speed is 4m3/ min, feeding speed 35mL/min, drying to water content are
4wt% obtains the pyracantha fortuneana fruit powder with anticancer function.
The compound drying aid is mixed by lactalbumin, beta-cyclodextrin, soluble starch 1:1:1 in mass ratio.
Embodiment 8
A kind of preparation method of the pyracantha fortuneana fruit powder with anticancer function, comprising the following steps:
I, pretreatment: ripe very likely, no disease and pests harm fresh firethorn fruit, the fire after being cleaned after cleaning up are chosen
Spine fruit；Pyracantha fortuneana fruit after cleaning is placed in 3 DEG C, is crushed 10min in the pulverizer of 4500r/min, is subsequently placed into 10 DEG C, 1800r/
Glue mill is carried out in the rubber mill of min obtains pyracantha fortuneana pulp after glue grinds 20min；
II, enzymatic hydrolysis: sodium citrate, Ascorbic acid chitosan quaternary ammonium salt, sodium chloride, lemon is added into above-mentioned pyracantha fortuneana fruit slurry
Lemon acid, the pyracantha fortuneana pulp, sodium citrate, Ascorbic acid chitosan quaternary ammonium salt, sodium chloride, citric acid mass ratio be 100:
0.015:0.015:0.02:0.02 stirs 15min at 25 DEG C with the revolving speed of 400r/min, obtains pyracantha fortuneana colour protecting liquid；Then to
In pyracantha fortuneana colour protecting liquid be added pectase, cellulase, the pyracantha fortuneana colour protecting liquid, pectase, cellulase mass ratio be 50:
0.02:0.02 is put into the interior enzymatic hydrolysis 2h of water bath chader of 40 DEG C, 100r/min, obtains pyracantha fortuneana enzymolysis liquid；
III, filtering: processing is filtered to above-mentioned pyracantha fortuneana enzymolysis liquid using 100 mesh filter clothes, the residue in pulp is removed, obtains
To pyracantha fortuneana filtered fluid, it is subsequently added into the compound drying aid of pyracantha fortuneana filtered fluid quality 4.5%, with the revolving speed of 400r/min at 25 DEG C
25min is stirred, pyracantha fortuneana mixed liquor is obtained；
IV, is dry: carrying out spray drying treatment, the inlet air temperature and outlet air of the spray drying to above-mentioned pyracantha fortuneana mixed liquor
Temperature is respectively 150 DEG C, 70 DEG C, and hot wind wind speed is 4m3/ min, feeding speed 35mL/min, drying to water content are
4wt% obtains the pyracantha fortuneana fruit powder with anticancer function.
The compound drying aid is mixed by lactalbumin, beta-cyclodextrin, soluble starch 1:1:1 in mass ratio.
Embodiment 9
A kind of preparation method of the pyracantha fortuneana fruit powder with anticancer function, comprising the following steps:
I, pretreatment: ripe very likely, no disease and pests harm fresh firethorn fruit, the fire after being cleaned after cleaning up are chosen
Spine fruit；Pyracantha fortuneana fruit after cleaning is placed at 3 DEG C, 3h is pre-chilled, then pyracantha fortuneana fruit is carried out freezing to locate using subzero 80 DEG C of liquid nitrogen
Reason, freeze-off time 15min, the fruit freezed is placed at subzero 10 DEG C and refrigerates 48h, it is then allowed to thaw at 3 DEG C, when defrosting
Between 9h, the pyracantha fortuneana fruit after being thawed；Pyracantha fortuneana fruit after above-mentioned defrosting is placed in 3 DEG C, is crushed in the pulverizer of 4500r/min
10min is subsequently placed into 10 DEG C, carries out glue mill in the rubber mill of 1800r/min and obtain pyracantha fortuneana pulp after glue grinds 20min；
II, enzymatic hydrolysis: sodium citrate, L-cysteine hydrochloride, Ascorbic acid shell are added into above-mentioned pyracantha fortuneana fruit slurry and gathers
Sugared quaternary ammonium salt, sodium chloride, citric acid, the pyracantha fortuneana pulp, sodium citrate, L-cysteine hydrochloride, Ascorbic acid shell are poly-
Sugared quaternary ammonium salt, sodium chloride, citric acid mass ratio be 100:0.01:0.01:0.01:0.02:0.02, with 400r/ at 25 DEG C
The revolving speed of min stirs 15min, obtains pyracantha fortuneana colour protecting liquid；Then pectase, cellulase are added into pyracantha fortuneana colour protecting liquid, it is described
Pyracantha fortuneana colour protecting liquid, pectase, cellulase mass ratio be 50:0.02:0.02, be put into 40 DEG C, the water-bath of 100r/min oscillation
2h is digested in device, obtains pyracantha fortuneana enzymolysis liquid；
III, filtering: processing is filtered to above-mentioned pyracantha fortuneana enzymolysis liquid using 100 mesh filter clothes, the residue in pulp is removed, obtains
To pyracantha fortuneana filtered fluid, it is subsequently added into the compound drying aid of pyracantha fortuneana filtered fluid quality 4.5%, with the revolving speed of 400r/min at 25 DEG C
25min is stirred, pyracantha fortuneana mixed liquor is obtained；
IV, is dry: carrying out spray drying treatment, the inlet air temperature and outlet air of the spray drying to above-mentioned pyracantha fortuneana mixed liquor
Temperature is respectively 150 DEG C, 70 DEG C, and hot wind wind speed is 4m3/ min, feeding speed 35mL/min, drying to water content are
4wt% obtains the pyracantha fortuneana fruit powder with anticancer function.
The compound drying aid by lactalbumin, modified beta-cyclodextrin, soluble starch 1:1:1 in mass ratio mixing and
At.
The modified beta-cyclodextrin the preparation method comprises the following steps: by mass parts weigh 4 parts of beta-cyclodextrins, 0.5 part of maleic acid,
6 parts of sodium dihydric hypophosphites, 0.01 part of adjacent methyl hydroquinone, stir 25min at 25 DEG C with the revolving speed of 400r/min, are mixed
Close material；Then 40wt% ethanol water is added into mixture by mass volume ratio 1:3 (g/mL), with 400r/ at 25 DEG C
The revolving speed of min stirs 25min, then is placed at 100 DEG C and reacts 3h, is cooled to 25 DEG C, obtains reactant；By the matter of 1:3 (g/mL)
Dehydrated alcohol is added into reactant for amount volume ratio, stands for 24 hours, filters, and washing filter cake repeatedly with dehydrated alcohol to pH is 7, connects
Filter cake is placed at 55 DEG C dry 14h to get modified beta-cyclodextrin.
Embodiment 10
A kind of preparation method of the pyracantha fortuneana fruit powder with anticancer function, comprising the following steps:
I, pretreatment: ripe very likely, no disease and pests harm fresh firethorn fruit, the fire after being cleaned after cleaning up are chosen
Spine fruit；Pyracantha fortuneana fruit after cleaning is placed at 3 DEG C, 3h is pre-chilled, then pyracantha fortuneana fruit is carried out freezing to locate using subzero 80 DEG C of liquid nitrogen
Reason, freeze-off time 15min, the fruit freezed is placed at subzero 10 DEG C and refrigerates 48h, it is then allowed to thaw at 3 DEG C, when defrosting
Between 9h, the pyracantha fortuneana fruit after being thawed；Pyracantha fortuneana fruit after above-mentioned defrosting is placed in 3 DEG C, is crushed in the pulverizer of 4500r/min
10min is subsequently placed into 10 DEG C, carries out glue mill in the rubber mill of 1800r/min and obtain pyracantha fortuneana pulp after glue grinds 20min；
II, enzymatic hydrolysis: sodium citrate, L-cysteine hydrochloride, Ascorbic acid shell are added into above-mentioned pyracantha fortuneana fruit slurry and gathers
Sugared quaternary ammonium salt, sodium chloride, citric acid, the pyracantha fortuneana pulp, sodium citrate, L-cysteine hydrochloride, Ascorbic acid shell are poly-
Sugared quaternary ammonium salt, sodium chloride, citric acid mass ratio be 100:0.01:0.01:0.01:0.02:0.02, with 400r/ at 25 DEG C
The revolving speed of min stirs 15min, obtains pyracantha fortuneana colour protecting liquid；Then pectase, cellulase are added into pyracantha fortuneana colour protecting liquid, it is described
Pyracantha fortuneana colour protecting liquid, pectase, cellulase mass ratio be 50:0.02:0.02, be put into 40 DEG C, the water-bath of 100r/min oscillation
2h is digested in device, obtains pyracantha fortuneana enzymolysis liquid；
III, filtering: processing is filtered to above-mentioned pyracantha fortuneana enzymolysis liquid using 100 mesh filter clothes, the residue in pulp is removed, obtains
To pyracantha fortuneana filtered fluid, it is subsequently added into the compound drying aid of pyracantha fortuneana filtered fluid quality 4.5%, with the revolving speed of 400r/min at 25 DEG C
25min is stirred, pyracantha fortuneana mixed liquor is obtained；
IV, is dry: carrying out vacuum freeze drying processing to pyracantha fortuneana mixed liquor, the condition of vacuum freeze drying is control material
Thickness 10mm sets pre-freezing temperature as -20 DEG C, keeps 2h after sample temperature drops to set temperature, sets sublimation temperature as 10
DEG C, resolution temperature is 35 DEG C, absolute pressure 30Pa, and drying to water content is 30wt%, obtains the pyracantha fortuneana powder of primary drying；It is right
The pyracantha fortuneana powder of gained primary drying carries out spray drying treatment, and the inlet air temperature and leaving air temp of the spray drying are respectively
150 DEG C, 70 DEG C, hot wind wind speed are 4m3/ min, feeding speed 35mL/min, drying to water content are 4wt%, are had
The pyracantha fortuneana fruit powder of anticancer function.
The compound drying aid by lactalbumin, modified beta-cyclodextrin, soluble starch 1:1:1 in mass ratio mixing and
At.
The modified beta-cyclodextrin the preparation method comprises the following steps: by mass parts weigh 4 parts of beta-cyclodextrins, 0.5 part of maleic acid,
6 parts of sodium dihydric hypophosphites, 0.01 part of adjacent methyl hydroquinone, stir 25min at 25 DEG C with the revolving speed of 400r/min, are mixed
Close material；Then 40wt% ethanol water is added into mixture by mass volume ratio 1:3 (g/mL), with 400r/ at 25 DEG C
The revolving speed of min stirs 25min, then is placed at 100 DEG C and reacts 3h, is cooled to 25 DEG C, obtains reactant；By the matter of 1:3 (g/mL)
Dehydrated alcohol is added into reactant for amount volume ratio, stands for 24 hours, filters, and washing filter cake repeatedly with dehydrated alcohol to pH is 7, connects
Filter cake is placed at 55 DEG C dry 14h to get modified beta-cyclodextrin.
Comparative example 1
A kind of preparation method of the pyracantha fortuneana fruit powder with anticancer function, comprising the following steps:
I, pretreatment: ripe very likely, no disease and pests harm fresh firethorn fruit, the fire after being cleaned after cleaning up are chosen
Spine fruit；Pyracantha fortuneana fruit after cleaning is placed in 3 DEG C, is crushed 10min in the pulverizer of 4500r/min, is subsequently placed into 10 DEG C, 1800r/
Glue mill is carried out in the rubber mill of min obtains pyracantha fortuneana pulp after glue grinds 20min；
II, enzymatic hydrolysis: sodium citrate, L-cysteine hydrochloride, Ascorbic acid shell are added into above-mentioned pyracantha fortuneana fruit slurry and gathers
Sugared quaternary ammonium salt, sodium chloride, citric acid, the pyracantha fortuneana pulp, sodium citrate, L-cysteine hydrochloride, Ascorbic acid shell are poly-
Sugared quaternary ammonium salt, sodium chloride, citric acid mass ratio be 100:0.01:0.01:0.01:0.02:0.02, with 400r/ at 25 DEG C
The revolving speed of min stirs 15min, obtains pyracantha fortuneana colour protecting liquid；Then pectase, cellulase are added into pyracantha fortuneana colour protecting liquid, it is described
Pyracantha fortuneana colour protecting liquid, pectase, cellulase mass ratio be 50:0.02:0.02, be put into 40 DEG C, the water-bath of 100r/min oscillation
2h is digested in device, obtains pyracantha fortuneana enzymolysis liquid；
III, filtering: processing is filtered to above-mentioned pyracantha fortuneana enzymolysis liquid using 100 mesh filter clothes, the residue in pulp is removed, obtains
To pyracantha fortuneana filtered fluid；
IV, is dry: carrying out spray drying treatment, the inlet air temperature and outlet air of the spray drying to above-mentioned pyracantha fortuneana filtered fluid
Temperature is respectively 150 DEG C, 70 DEG C, and hot wind wind speed is 4m3/ min, feeding speed 35mL/min, drying to water content are
4wt% obtains the pyracantha fortuneana fruit powder with anticancer function.
Comparative example 2
A kind of preparation method of the pyracantha fortuneana fruit powder with anticancer function, comprising the following steps:
I, pretreatment: ripe very likely, no disease and pests harm fresh firethorn fruit, the fire after being cleaned after cleaning up are chosen
Spine fruit；Pyracantha fortuneana fruit after cleaning is placed in 3 DEG C, is crushed 10min in the pulverizer of 4500r/min, is subsequently placed into 10 DEG C, 1800r/
Glue mill is carried out in the rubber mill of min obtains pyracantha fortuneana pulp after glue grinds 20min；
II, enzymatic hydrolysis: sodium chloride, citric acid, the pyracantha fortuneana pulp, sodium chloride, citric acid is added into above-mentioned pyracantha fortuneana fruit slurry
Mass ratio be 100:0.02:0.02,15min is stirred with the revolving speed of 400r/min at 25 DEG C, obtains pyracantha fortuneana colour protecting liquid；Then
Into pyracantha fortuneana colour protecting liquid be added pectase, cellulase, the pyracantha fortuneana colour protecting liquid, pectase, cellulase mass ratio be 50:
0.02:0.02 is put into the interior enzymatic hydrolysis 2h of water bath chader of 40 DEG C, 100r/min, obtains pyracantha fortuneana enzymolysis liquid；
III, filtering: processing is filtered to above-mentioned pyracantha fortuneana enzymolysis liquid using 100 mesh filter clothes, the residue in pulp is removed, obtains
To pyracantha fortuneana filtered fluid, it is subsequently added into the compound drying aid of pyracantha fortuneana filtered fluid quality 4.5%, with the revolving speed of 400r/min at 25 DEG C
25min is stirred, pyracantha fortuneana mixed liquor is obtained；
IV, is dry: carrying out spray drying treatment, the inlet air temperature and outlet air of the spray drying to above-mentioned pyracantha fortuneana mixed liquor
Temperature is respectively 150 DEG C, 70 DEG C, and hot wind wind speed is 4m3/ min, feeding speed 35mL/min, drying to water content are
4wt% obtains the pyracantha fortuneana fruit powder with anticancer function.
The compound drying aid is mixed by lactalbumin, beta-cyclodextrin, soluble starch 1:1:1 in mass ratio.
Test case 1
Collect the measurement of powder rate: referring to the periodical literature " Research on processing technology of Xuancheng's pawpaw fruit juice and fast dissolving fruit powder " of Han Jianqun
3.3.5 fruit powder physical property measurement method in section there is the pyracantha fortuneana fruit powder collection powder rate of anticancer function to be measured the present invention, the collection
The calculation formula of powder rate are as follows: amount × 100% of feed liquid solid content before the powder ÷ of collection powder rate (%)=collect after drying is dry,
Specific calculated result is shown in Table 1.
Table 1: collection powder rate test result table
| Group | Collect powder rate/% |
| Embodiment 1 | 34.1 |
| Embodiment 2 | 29.5 |
| Embodiment 3 | 30.1 |
| Embodiment 4 | 29.2 |
| Embodiment 5 | 39.9 |
| Embodiment 6 | 32.4 |
| Embodiment 7 | 33.0 |
| Embodiment 8 | 32.3 |
| Embodiment 9 | 41.2 |
| Embodiment 10 | 43.9 |
| Comparative example 1 | 24.7 |
Compound drying aid can improve powder-product quality, keep silty fine and smooth, not hygroscopic agglomeration, the instant effect of powder and stream
Dynamic property enhancing, has extensively in the processing of the products such as milk powder, milk tea, instant tea, instant coffee, red date powder, toppings and fruit vegetable powder
Application.By test result it is found that in embodiment 1 compound drying aid by lactalbumin, beta-cyclodextrin, soluble starch mixing and
At collection powder rate is higher than embodiment 2-4 (using any two kinds in lactalbumin, beta-cyclodextrin, soluble starch as compound
Drying aid) and using the comparative example 1 of compound drying aid；And embodiment 5 is modified beta-cyclodextrin, relative to embodiment 1
For, further improve the collection powder rate of the pyracantha fortuneana fruit powder with anticancer function.
Test case 2
The inhibiting rate of cancer cell multiplication: with reference to journal of writings " proliferation suppression of the Ah pa for Buddhist nun to hepatocellular carcinoma H22 of Chen Tao
Production use " in detection method, using the MTT colorimetric determination present invention have anticancer function pyracantha fortuneana fruit powder to human liver cancer cell
The inhibited proliferation of HepG2.The wherein cultural method of cancer cell are as follows: human hepatoma cell strain Hep G2 is (purchased from Chinese section
Institute cell institute (Shanghai) cell bank) it is cultivated with the RPMI1640 containing 10% fetal calf serum and 100 μ g/ml Pen .- Streps
Base (is purchased from U.S. HyClone company), is placed in 37 DEG C, 5%CO2, constant-temperature enclosed incubator under the conditions of saturated humidity is (purchased from beauty
NBS company of state) in cultivate and pass on, every 3-4d change liquid passage.Experimental method: to 96 holes for being vaccinated with human liver cancer cell HepG2
The pyracantha fortuneana fruit powder with anticancer function is added in tissue culture plate, wherein the intervention concentration of the pyracantha fortuneana fruit powder with anticancer function is
Absorbance is measured after 300 μ g/mL, 72h and calculates the inhibiting rate of cancer cell multiplication, and specific calculated result is shown in Table 2.
Table 2: the inhibiting rate of cancer cell multiplication
| Group | Inhibiting rate/% of cancer cell multiplication |
| Embodiment 1 | 61.5 |
| Embodiment 2 | 57.8 |
| Embodiment 3 | 58.4 |
| Embodiment 4 | 57.5 |
| Embodiment 5 | 65.2 |
| Embodiment 6 | 53.7 |
| Embodiment 7 | 52.2 |
| Embodiment 8 | 54.5 |
| Embodiment 9 | 74.0 |
| Embodiment 10 | 86.1 |
| Comparative example 2 | 45.4 |
By test result it is found that the use of embodiment 1 is gathered by sodium citrate, L-cysteine hydrochloride, Ascorbic acid shell
The protective agent that sugared quaternary ammonium salt mixes, the inhibiting rate of cancer cell multiplication be higher than embodiment 6-8 (protective agent by sodium citrate,
Any two kinds of L-cysteine hydrochloride, Ascorbic acid chitosan quaternary ammonium salt mix) and not using protective agent processing
Comparative example 2；And pyracantha fortuneana fruit passes through first freezing processing natural thaw again in embodiment 9, reduces activity in fresh fruit during the preparation process
The loss of ingredient improves the inhibiting rate of cancer cell multiplication for embodiment 1；Further, embodiment 10, which uses, divides
The dry method of section is dried, and prevents the excessively high destruction active constituent of temperature of charge, for embodiment 9, preparation
The pyracantha fortuneana fruit powder with anticancer function out has the inhibiting rate of higher cancer cell multiplication.
The above described is only a preferred embodiment of the present invention, limitation in any form not is done to the present invention, therefore
The equivalent or simple change that all principles described according to the invention patent design are done, is included in the scope of protection of the patent of the present invention
It is interior；Those skilled in the art can make various modifications or additions to the described embodiments
Or be substituted in a similar manner, without departing from structure of the invention or beyond the scope defined by this claim,
It is within the scope of protection of the invention.
Claims (9)
1. a kind of preparation method of the pyracantha fortuneana fruit powder with anticancer function, which comprises the following steps:
I, pretreatment: ripe very likely, no disease and pests harm fresh firethorn fruit, the pyracantha fortuneana after being cleaned after cleaning up are chosen
Fruit；First the pyracantha fortuneana fruit after cleaning is placed at 2-6 DEG C and carries out break process, is subsequently placed into rubber mill and carries out glue mill, obtain fire
Spine pulp；
II, enzymatic hydrolysis: protective agent, sodium chloride, citric acid, the pyracantha fortuneana pulp, protective agent, chlorination is added into above-mentioned pyracantha fortuneana fruit slurry
Sodium, citric acid mass ratio be 100:(0.03-0.08): (0.01-0.04): (0.02-0.05), mix, obtain pyracantha fortuneana color protection
Liquid；Then be added complex enzyme into pyracantha fortuneana colour protecting liquid, the pyracantha fortuneana colour protecting liquid, complex enzyme mass ratio be 50:(0.02-
0.06) 1.5-3.5h, is digested at 30-45 DEG C, obtains pyracantha fortuneana enzymolysis liquid；
III, filtering: processing is filtered to above-mentioned pyracantha fortuneana enzymolysis liquid, the residue in pulp is removed, obtains pyracantha fortuneana filtered fluid, then
The compound drying aid of pyracantha fortuneana filtered fluid quality 3-9% is added, pyracantha fortuneana mixed liquor is obtained after mixing；
IV, is dry: carrying out spray drying treatment to above-mentioned pyracantha fortuneana mixed liquor, drying to water content is 2-6wt%, obtains having anti-
The pyracantha fortuneana fruit powder of cancer effect.
2. the preparation method of the pyracantha fortuneana fruit powder with anticancer function as described in claim 1, which is characterized in that the protective agent packet
Include one of sodium citrate, L-cysteine hydrochloride, Ascorbic acid chitosan quaternary ammonium salt or a variety of.
3. the preparation method of the pyracantha fortuneana fruit powder with anticancer function as described in claim 1, which is characterized in that the complex enzyme packet
Include pectase, cellulase, hemicellulase, two or more in zytase.
4. as described in claim 1 with anticancer function pyracantha fortuneana fruit powder preparation method, which is characterized in that it is described it is compound help it is dry
Agent includes lactalbumin, beta-cyclodextrin, modified beta-cyclodextrin, two or more in soluble starch.
5. the preparation method of the pyracantha fortuneana fruit powder with anticancer function as claimed in claim 4, which is characterized in that the modified β-ring
Dextrin the preparation method comprises the following steps: weighing 2-5 parts of beta-cyclodextrins, 0.3-0.7 parts of maleic acids, 3-10 parts of hypophosphorous acid two by mass parts
Hydrogen sodium, 0.01-0.03 parts of adjacent methyl hydroquinones, mixing obtain mixture；By mass volume ratio 1:(2-4) (g/mL) to mixed
It closes and 40wt%-60wt% ethanol water is added in material, mixing, which is placed at 100 DEG C -120 DEG C, carries out reaction 2-6h, after cooling
Reactant being obtained, then by 1:(3-5) dehydrated alcohol is added into reactant the mass volume ratio of (g/mL), stands, filters, and it is dry
Afterwards up to modified beta-cyclodextrin.
6. the preparation method of the pyracantha fortuneana fruit powder with anticancer function as described in claim 1, which is characterized in that the step I is pre-
Processing are as follows: choose ripe very likely, no disease and pests harm fresh firethorn fruit, the pyracantha fortuneana fruit after being cleaned after cleaning up；First will
Pyracantha fortuneana fruit after cleaning, which is placed at 0-4 DEG C, is pre-chilled 2-5h, freezes 10- under being then placed in subzero 120 DEG C-subzero 20 DEG C of liquid nitrogen
25min, the fruit freezed are put at subzero 5 DEG C of subzero 15- after refrigeration 12-60h, are placed at 2-6 DEG C and thaw, solved
Pyracantha fortuneana fruit after jelly；Pyracantha fortuneana fruit after above-mentioned defrosting is placed at 2-6 DEG C and carries out break process, is subsequently placed into rubber mill and carries out
Glue mill, obtains pyracantha fortuneana pulp.
7. the preparation method of the pyracantha fortuneana fruit powder with anticancer function as described in claim 1, which is characterized in that the step IV is dry
It is dry are as follows: vacuum freeze drying processing first to be carried out to pyracantha fortuneana mixed liquor, drying to water content is 20-40wt%, obtains primary drying
Pyracantha fortuneana powder；Then spray drying treatment is carried out to the pyracantha fortuneana powder of gained primary drying, drying to water content is 2-6wt%, is obtained
To the pyracantha fortuneana fruit powder with anticancer function.
8. the preparation method of the pyracantha fortuneana fruit powder with anticancer function as claimed in claim 7, which is characterized in that the spray drying
Inlet air temperature and leaving air temp controlled respectively at 145 DEG C -165 DEG C, 65-75 DEG C.
9. a kind of pyracantha fortuneana fruit powder with anticancer function, which is characterized in that anti-using having described in any one of claim 1-8
The preparation method of the pyracantha fortuneana fruit powder of cancer effect is prepared.
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