CN109170532A - A kind of preparation method and application of Semen Coicis extract - Google Patents
A kind of preparation method and application of Semen Coicis extract Download PDFInfo
- Publication number
- CN109170532A CN109170532A CN201811050338.2A CN201811050338A CN109170532A CN 109170532 A CN109170532 A CN 109170532A CN 201811050338 A CN201811050338 A CN 201811050338A CN 109170532 A CN109170532 A CN 109170532A
- Authority
- CN
- China
- Prior art keywords
- semen coicis
- slurries
- polysaccharide
- protein
- coixlacrymajobi powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000000582 semen Anatomy 0.000 title claims abstract description 197
- 239000000284 extract Substances 0.000 title claims abstract description 37
- 238000002360 preparation method Methods 0.000 title claims abstract description 25
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 143
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 143
- 150000004676 glycans Chemical class 0.000 claims abstract description 131
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 128
- 239000005017 polysaccharide Substances 0.000 claims abstract description 128
- 239000002002 slurry Substances 0.000 claims abstract description 106
- 239000000843 powder Substances 0.000 claims abstract description 72
- 244000077995 Coix lacryma jobi Species 0.000 claims abstract description 70
- 235000007354 Coix lacryma jobi Nutrition 0.000 claims abstract description 69
- 239000003463 adsorbent Substances 0.000 claims abstract description 54
- 229920000620 organic polymer Polymers 0.000 claims abstract description 47
- 238000005238 degreasing Methods 0.000 claims abstract description 32
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- 239000013527 degreasing agent Substances 0.000 claims abstract description 17
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 58
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- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 24
- 239000011347 resin Substances 0.000 claims description 16
- 229920005989 resin Polymers 0.000 claims description 16
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims description 14
- 240000002853 Nelumbo nucifera Species 0.000 claims description 13
- 235000006508 Nelumbo nucifera Nutrition 0.000 claims description 13
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- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 12
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- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 9
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- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 8
- 235000010413 sodium alginate Nutrition 0.000 claims description 8
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- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 claims description 6
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- 238000001291 vacuum drying Methods 0.000 claims description 5
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 claims description 4
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- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 claims description 3
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 claims description 3
- 229910052783 alkali metal Inorganic materials 0.000 claims description 3
- -1 alkali metal hydrogencarbonate Chemical class 0.000 claims description 3
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- BHZOKUMUHVTPBX-UHFFFAOYSA-M sodium acetic acid acetate Chemical compound [Na+].CC(O)=O.CC([O-])=O BHZOKUMUHVTPBX-UHFFFAOYSA-M 0.000 claims description 3
- 229940095064 tartrate Drugs 0.000 claims description 2
- 210000004885 white matter Anatomy 0.000 claims description 2
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims 1
- 240000008866 Ziziphus nummularia Species 0.000 claims 1
- GCNLQHANGFOQKY-UHFFFAOYSA-N [C+4].[O-2].[O-2].[Ti+4] Chemical compound [C+4].[O-2].[O-2].[Ti+4] GCNLQHANGFOQKY-UHFFFAOYSA-N 0.000 claims 1
- 229910017604 nitric acid Inorganic materials 0.000 claims 1
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/12—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from cereals, wheat, bran, or molasses
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/10—General methods of cooking foods, e.g. by roasting or frying
- A23L5/17—General methods of cooking foods, e.g. by roasting or frying in a gaseous atmosphere with forced air or gas circulation, in vacuum or under pressure
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/27—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Abstract
The present invention relates to a kind of preparation methods of Semen Coicis extract, specifically comprise the following steps: S1, drying and crushing coixlacrymajobi powder；S2, acid processing and the starch being added in degreasing agent removal coixlacrymajobi powder and lipid are utilized；S3, Semen Coicis slurries are handled by dynamic super-voltage micro jet, the dissolution of polysaccharide and protein in Semen Coicis slurries is promoted to extract；S4, the mass concentration for adjusting Semen Coicis slurries are added ionic strength adjustor, adjust the ionic strength of Semen Coicis slurries, while adjusting the pH of Semen Coicis slurries；S5, organic polymer adsorbent is added in Semen Coicis slurries, protein and polysaccharide aggregate are adsorbed；S6, filtering is stood, collects the organic polymer adsorbent filtered out, successively elute polysaccharide and protein；S7, starch and lipid are removed again by low temperature sedimentation and centrifugal degreasing, it is final that the Semen Coicis lotion rich in polysaccharide and protein is made.Instant compound coixlacrymajobi powder is formed using Semen Coicis latex composite compounding obtained.
Description
Technical field
The invention belongs to food processing technology field, active substance of plant extractions, manufacture field, and in particular to a kind of Semen Coicis
Method for preparing extractive and application.
Background technique
Semen Coicis also known as semen coicis, adlay, for 1 year raw or herbaceos perennial adlay kernel.Semen Coicis Yushan Hills a variety of greatly
Ground just has long cultivation history in In Fujian Province.Men of old regards Semen Coicis as in the treasure of nature, for offering a sacrifice to gods or ancestors；It is modern
Semen Coicis is considered as midsummer full of nutrition and relieved summer heat good merchantable brand by people, and it can be served, and pharmaceutically acceptable.Semen Coicis protein rich in, carbon
The content of hydrate and fat is higher, also rich in having polysaccharide, B family vitamin and several mineral materials, have hypoglycemic, anticancer,
Promote the multiple efficacies such as immunological regulation.However, at present Semen Coicis with give birth to rice sale based on, development of exploitation level is low, added value of product
It is not high.
Semen Coicis is rich in essential amino acid and composition of amino acid is reasonable, is closer to human body requirements；Human body must
Amino acid is needed, refers to that human body cannot synthesize or aggregate velocity is far not suitable with the needs of body, it is necessary to be supplied by food proteins, these ammonia
Base acid is known as essential amino acid.The content of protein is about the 15%~20% of the seed of jog's tears in Semen Coicis, containing 8 kinds necessary to human body
Amino acid accounts for about the 5% of seed of jog's tears quality, it can be seen that, Semen Coicis albumen is high-quality protein, is had to promoting health extremely
Benefit.
Plant polyose be plant metabolism generate the degree of polymerization be more than 10 glycan, many identical or different monosaccharide with
Compound composed by α-or β-glycosidic bond, international scientific circle even propose that 21 century is the century of polysaccharide；Scientific investigations showed that
Many plant polyoses have bioactivity, such as hypoglycemic, reducing blood lipid, immunological regulation, antitumor, anti-radiation, anti-bacteria and anti-virus, guarantor
The dirty equal health-care effects of protect liver.Some researches show that, polyoses content is also higher in Semen Coicis, and about 1%~3%；Semen Coicis polysaccharide has one
Fixed hypoglycemic effect is that can inhibit hepatic glycogenolytic, muscle glycogen glycolysis due to it, to reach the mesh of blood glucose level reduction
's.
In conclusion how the extraction of Semen Coicis albumen and polysaccharide is for be mankind's health service with important using Semen Coicis
Meaning.Extraction in existing technology about Semen Coicis albumen generallys use extraction, enzymatic isolation method etc.；Extraction will
After Semen Coicis crushes, hydrochloric acid solution is added and is mixed into mixed liquor, reacts a few hours under stiring, is filtered out residue using filter bag,
Filtrate is neutralized to neutrality with lye again, collect solid component after solution that finally will be neutral is dry, Semen Coicis albumen, utilize
Extraction needs to expend a large amount of acid & alkali liquid, and the yield of Semen Coicis albumen is low, purity is low and has peculiar smell；Publication No.
The Chinese invention of CN102838666A discloses a kind of method for extracting coix seed protein by enzymolysis, and smashed Semen Coicis and EM is compound
Enzyme aqueous solution mixing；Mixed liquor is using ultrasonication and heats, and the pH for adjusting mixed liquor ferments to form fermentation mixed liquor, filtering
Ferment mixed liquor taking precipitate, finally obtains Semen Coicis albumen using far infrared constant temperature drying box dry sediment；Publication No.
The Chinese patent of 106620393A discloses polyphenol extract and preparation method in a kind of Semen Coicis, more using extract by solvents Semen Coicis
Phenol combines centrifugation, rotary evaporation, freeze-drying to be extracted from Semen Coicis rich in polyphenol by adding extraction solvent after mechanical shaking extraction
Powder.The Chinese patent of Publication No. CN102504038B discloses a kind of plant polyose and preparation method thereof and in cigarette
Application, mentioned by water, be mixed to prepare extracting solution with water after plant is crushed, by centrifugation and vacuum filtration, concentration etc. processes
Concentrate is obtained, finally obtains plant polyose by ethanol solution extraction, freeze-drying.From Semen Coicis single extraction protein,
Polysaccharide or phenolic compound existing research, but lack and compound collecting or continuously extracted a variety of compound from Semen Coicis using complex art
Ingredient.
Summary of the invention
In view of above-mentioned deficiencies of the prior art, the present invention is intended to provide a kind of Semen Coicis extract and preparation method thereof with answer
With can synchronize the protein and polysaccharide that extract in Semen Coicis by complex art, shorten process flow and process time.
Technical scheme is as follows:
A kind of preparation method of Semen Coicis extract, specifically comprises the following steps:
S1, Semen Coicis particle is placed in drying in drying box, drying temperature is 60~70 DEG C；After crushing drying using pulverizer
Semen Coicis particle, and cross 220 mesh screens, obtain dry coixlacrymajobi powder；
S2, it is directly added into edible organic acid extraction in coixlacrymajobi powder, handles the starch in removal coixlacrymajobi powder using acid, then
Degreasing agent is added in coixlacrymajobi powder solution after acidification and carries out degreasing pretreatment, removes desizing and rouge with membrane filtration after degreasing
Class finally dries coixlacrymajobi powder under the conditions of 60~70 DEG C；
S3, deionized water is added in except the drying coixlacrymajobi powder after starch and degreasing, solid-liquid ratio is 1:40~60, will be mixed
Uniform Semen Coicis slurries are placed in Microfluidizer to be handled by dynamic super-voltage micro jet, and then promotes polysaccharide in coixlacrymajobi powder
Dissolution with protein is extracted；
S4, the deionized water by being added in deionized water or evaporation slurries, and then adjust and penetrated by dynamic super-voltage is micro-
The concentration of the Semen Coicis slurries of stream process is added ionic strength adjustor, makes it so that the mass concentration of Semen Coicis slurries is 2~4%
It is uniformly mixed with Semen Coicis slurries, the ionic strength for adjusting Semen Coicis slurries is lower than 0.4M, while extracting in sample detection Semen Coicis slurries
The iso-electric point of protein and the dissociation number pKa of polysaccharide, and according to the pH of measured value adjusting Semen Coicis slurries, make the pH of Semen Coicis slurries
Greater than the iso-electric point of protein, less than the dissociation number pKa of polysaccharide, promote protein and polysaccharide agglomerates into aggregate；
Organic polymer adsorbent is added in S5, Semen Coicis slurries after adjustment, to the aggregate of protein and polysaccharide into
Row absorption, is aided with the ultrasonic treatment of 30~40min in adsorption process, and supersonic frequency is 100~150w；The organic polymer
The additive amount of adsorbent and the volume ratio of Semen Coicis slurries are 1:10~12, and adsorption temp is 40~50 DEG C, sorption reaction time 1
~2h；
S6, it is filtered after standing the Semen Coicis slurries after adsorption reaction, collects the organic polymer adsorbent filtered out, successively use
Deionized water and deionized water elution polysaccharide and protein containing sodium chloride solution, the polysaccharide and protein finally eluted
It is dissolved in deionized water；Filtering and elution process are equivalent to the concentration and enrichment of protein and polysaccharide；
S7, the deionized water solution containing polysaccharide and protein is placed in 20 DEG C of low temperature environments below makes in solution not
The starch of removing settles, and takes after supernatant again through supercentrifuge secondary degreasing, final to be made rich in polysaccharide and protein
Semen Coicis lotion.
Wherein, it is further comprised the steps of: between step S3 and S4
S3-1: being added macroporous absorbent resin in uniformly mixed Semen Coicis slurries, after adsorbing the impurity in Semen Coicis slurries, mistake
It filters Semen Coicis slurries and obtains filtrate.
Wherein, edible organic acid described in step S2 is one of citric acid and tartaric acid；It is described acid processing the time be
30~40min, temperature are 35~45 DEG C；The degreasing agent is alkali metal hydroxide, alkali carbonate or alkali metal carbonic acid
One of hydrogen salt is a variety of.
Wherein, the processing pressure of the processing of super-voltage micro jet described in step S3 is 60~160Mpa, treatment temperature 80
~90 DEG C, number of processes is 1~3 time.
Wherein, ionic strength adjustor described in step S4 is the mixed of neutral indifferent electrolyte, buffer and screening agent
Close solution；The indifferent electrolyte is one of sodium chloride, potassium nitrate or sodium perchlorate or a variety of；The buffer is vinegar
Acid-one of sodium acetate buffer agent or PBS buffer, for adjusting the pH of Semen Coicis slurries；The screening agent be citrate,
One of acetate or tartrate are a variety of；.
Wherein, the organic polymer adsorbent in step S5 is made of component A and B component, the component A be chitosan or
One of sodium alginate；The B component is one of cationic liquid polymer material or cation exchange resin；It is described
The volume ratio of component A and B component is 3:1.
Wherein, the organic polymer adsorbent preparation method specifically includes the following steps:
A1, chitosan or sodium alginate are dissolved with acetic acid solution, uniform dispersion liquid is made, in chitosan or sodium alginate
It is added in dispersion liquid, chitosan microball is prepared using emulsion-crosslinking method；
A2, chitosan microball is put into plasma activation instrument, under conditions of pressure is 40~80Pa, with plasma two
Carbon oxide gas handles 300~500s, the chitosan microball after being activated；
A3, using chitosan microball obtained in A2 as kernel, after surface grafting polymerization reacts, acquisition surface is had
The organic polymer adsorbent of macromolecule cation exchanger resin.
Wherein, the organic polymer adsorbent after eluting protein and polysaccharide is carried out organic by the method for electrolysis and ultrasound
The adsorbent reactivation of high polymer realizes recycling for adsorbent.
Wherein, compound coixlacrymajobi powder is mixed by the raw material of following parts by weight: 200-400 parts of Semen Coicis lotion, jujube water mention
100-200 parts of liquid, 50-70 parts of tea polysaccharide, 50-70 parts of polysaccharides, 50-70 parts of corn silk polysaccharide are not necessarily to external adding water；It is described multiple
The mixed solution of coixlacrymajobi powder is closed by vacuum concentration, vacuum drying, crushing and sterilizing, final instant compound coixlacrymajobi powder.
The invention has the following beneficial effects:
1, the present invention realizes the group of protein and polysaccharide in Semen Coicis slurries by itself physicochemical property of adjusting Semen Coicis slurries
It is poly-, while organic polymer adsorbent is added, protein and polysaccharide aggregate are adsorbed and is desorbed, it can be achieved at the same time egg
White matter and polysaccharide isolate and purify and enrichment method, realize the protein extraction synchronous with polysaccharide in Semen Coicis.
2, the present invention finally compounds the low starch of compound coixlacrymajobi powder needs and low fat using Semen Coicis extract, because starch contains
Amount is high, unfavorable to inhibition blood glucose rise；Fatty easily consumption loses and influences the shelf lifes such as coixlacrymajobi powder, also will affect compound coixlacrymajobi powder
The brew of product；Therefore by acidification and degreasing agent degreasing is added before the extraction of Semen Coicis polysaccharide and protein in the present invention
Process Semen Coicis slurries are pre-processed, remove Semen Coicis slurries in starch and fat；It is synchronous with protein in polysaccharide simultaneously
The starch and fat removed in extracting solution again is settled and is centrifugated after extraction by low temperature；It is secondary to remove starch and ungrease treatment
It is extracted respectively in compared to the prior art after protein and polysaccharide through enzymatic hydrolysis or centrifugation removal starch and degreasing, can be as far as possible
The starch removal rate and degreasing rate in final Semen Coicis lotion are improved, the quality of compound coixlacrymajobi powder is improved；It utilizes at acid before extraction
Reason carries out removing starch for the first time, will not influence the quality of protein and polysaccharide using food acids, while can be by food acids tune
The physicochemical property of whole Semen Coicis slurries, the acidity of food acids is unlikely to lead to the hydrolysis of protein, and can promote protein and polysaccharide
Reunion and extraction, using degreasing agent carry out ungrease treatment, degreasing agent is added after being acidified Semen Coicis slurries, Semen Coicis can be promoted to starch
Pigment and amine in liquid are separated in a salt form, accelerate degreasing speed, improve degreasing efficiency, while the degreasing being added
There is no the reagents that introducing is harmful to the human body for agent, have safety；It carries out removing starch and ungrease treatment, phase second after extraction
It is enriched with and is cleaned when in final Semen Coicis lotion obtained, it is ensured that the purity of Semen Coicis lotion.
3, the ion concentration and pH value of the invention by adjusting Semen Coicis slurries, so that the protein high molecular in Semen Coicis slurries
It can attract each other with polysaccharide polymer and agglomerate into complex coacervation object, it is compound to add the absorption of organic polymer adsorbent later
Condensation product, the mistake that the process of absorption, filtering and the desorption of organic polymer adsorbent mentions instead of traditional water, alkali carries or enzyme mention
Journey, while reducing extracting solution concentration and purification process, process flow and process time are shortened, protein is improved and polysaccharide mentions
Take rate, reduce too long process flow with successively extract protein caused by variation of the protein with extraction environments different when polysaccharide
With the loss of polysaccharide, the denaturation of protein and polysaccharide or other effective components in extraction process can be avoided.
4, the present invention is more using the compound jujube Aqueous extracts of Semen Coicis extract rich in protein and polysaccharide, tea polysaccharide, fructus lycii
Sugar, corn silk polysaccharide are processed into the instant coixlacrymajobi powder for blood pressure lowering, supplementing good protein.
Specific embodiment
Below with reference to preferred embodiment, the present invention is further illustrated.
Embodiment 1
A kind of preparation method of Semen Coicis extract, specifically comprises the following steps:
S1, Semen Coicis particle is placed in drying in drying box, drying temperature is 60 DEG C；The heart of a lotus seed after drying is crushed using pulverizer
Rice grain, and 220 mesh screens are crossed, obtain dry coixlacrymajobi powder；Select 220 mesh screens that can guarantee that the partial size of coixlacrymajobi powder is 0.06
Between~0.07mm, the smaller active constituent that can guarantee in Semen Coicis of the partial size of Semen Coicis can be dissolved out more effectively, if partial size
It is too small, phenomena such as will appear floating powder and extract, obtain dry coixlacrymajobi powder；
S2, it is directly added into edible organic acid extraction in coixlacrymajobi powder, handles the starch in removal coixlacrymajobi powder using acid；It is described
Edible organic acid is citric acid；The acid processing time is 30min, and temperature is 35 DEG C；Then, the coixlacrymajobi powder solution after acidification
Middle addition degreasing agent carries out degreasing pretreatment, and the degreasing agent is alkali metal hydroxide；It is removed and is formed sediment with membrane filtration after degreasing
Powder and lipid finally dry coixlacrymajobi powder under the conditions of 60 DEG C；Semen Coicis slurries after acidification are reacted with degreasing agent, Semen Coicis can be made to starch
Pigment and amine in liquid are separated in a salt form, accelerate degreasing speed, improve degreasing efficiency；
S3, in degreasing and except being added deionized water in the coixlacrymajobi powder after starch, solid-liquid ratio 1:40, the heart of a lotus seed that will be uniformly mixed
Rice & peanut milk liquid is placed in Microfluidizer to be handled by dynamic super-voltage micro jet, the processing pressure of the super-voltage micro jet processing
Power is 60Mpa, and treatment temperature is 80 DEG C, and number of processes is 1 time, is handled by super-voltage micro jet, and then promote in coixlacrymajobi powder
The dissolution of polysaccharide and protein is extracted；Dynamic super-voltage micro jet technology passes through strong shearing force, high-speed impact power and height
Frequency vibration is swung, and the function and effect of super-pressure are reached under relatively low pressure condition, is scattered in so that coixlacrymajobi powder is evengranular
In sample solution, so that Semen Coicis sample cell degree of crushing is further exacerbated by, cell resistance disappears, and intracellular effective component can
Quickly to dissolve into deionized water, be conducive to the progress extracted；
S4, deionized water is added or evaporates the deionized water in slurries, and then adjust by super-voltage micro jet processing
The concentration of Semen Coicis slurries is added ionic strength adjustor, makes itself and Semen Coicis slurries so that the mass concentration of Semen Coicis slurries is 2%
Be uniformly mixed, the ionic strength for adjusting Semen Coicis slurries is lower than 0.4M, while extract protein in sample detection Semen Coicis slurries etc.
The dissociation number pKa of electricity point PI and polysaccharide, and according to the pH of measured value adjusting Semen Coicis slurries, so that the pH of Semen Coicis slurries is greater than protein
Iso-electric point, less than the dissociation number pKa of polysaccharide；Protein and polysaccharide in Semen Coicis is soluble in water, in water due to protein
Thermodynamics is incompatible and cause mutually to separate between polysaccharide, generates two kinds of liquid-liquid separation phenomenons, i.e. discrete phase separation and association phase
Separation, when sample concentration is lower than 3~4wt%, solution system ionic strength is lower than 0.4M, and system pH is between isoelectric points of proteins
(PI) between polysaccharide dissociation number (pKa) when, two kinds of macromolecules have xenogenesis charge in solution, mutually inhale between two kinds of macromolecules
Draw to form complex coacervation object；The ionic strength adjustor is that the mixing of neutral indifferent electrolyte, buffer and screening agent is molten
Liquid；The indifferent electrolyte is sodium chloride；The buffer is Acetic acid-sodium acetate buffer, for adjusting the pH of Semen Coicis slurries,
So that the pH of Semen Coicis slurries is maintained in suitable range；The screening agent be citrate, for shelter, be complexed interference from
Son；
Organic polymer adsorbent is added in S5, Semen Coicis slurries after adjustment, to the aggregate of protein and polysaccharide into
Row absorption, is aided with the ultrasonic treatment of 30min, supersonic frequency 100w in adsorption process；The organic polymer adsorbent
The volume ratio of additive amount and Semen Coicis slurries is 1:10, and adsorption temp is 40 DEG C, sorption reaction time 1h；Protein and more at this time
The complex coacervation object of sugar can be adsorbed by organic polymer adsorbent；The organic polymer adsorbent has by component A and B component group
At the component A is chitosan derivatives, and B component is one of cation exchange resin；The body of the component A and B component
Product is than being 3:1；Chitosan derivatives are selected as the main component of organic polymer adsorbent, are because of chitosan macromolecular chain
On simultaneously containing two kinds of active reactive groups of amido and hydroxyl, different groups, preparation can be imported by various reactions
Different chitosan derivatives, the chitosan derivatives of formation, the group of importing can choose adsorbable protein and polysaccharide
Group, while chitosan Nantural non-toxic, absorption property is good, no adsorptive selectivity, can simultaneously protein and polysaccharide aggregate,
Therefore select chitosan as the main component of organic high molecular polymer adsorbent；Select cation exchange resin as B group
Part due in Semen Coicis slurries pH value be consistently greater than isoelectric points of proteins；Absorption behavior and egg of the protein on ion exchange resin
The net charge relationship of white matter is little, mainly with protein and the surface static electricity lotus property of contacts ionic exchange resin, number
Mesh is related；Protein surface not only carries positive (or negative) charge, but carries positive and negative charge simultaneously, only positive (or negative) charge
Quantity is opposite to be more than negative (or just) charge, simultaneously because the inhomogeneities of protein surface distribution of charges, when the equal electricity of protein
When selecting the pH lower than Semen Coicis slurries, although protein with negative net charge, still has asymmetric positive electrostatic charge distribution on molecule
It is contacted with cation exchange resin, therefore cation exchange resin can adsorb this proteinoid；
S6, it is filtered after standing the Semen Coicis slurries after adsorption reaction, collects the organic polymer adsorbent filtered out, successively use
Deionized water and containing sodium chloride solution deionized water elution polysaccharide and protein, final polysaccharide and protein be dissolved in deionization
In water, filtering and elution process are equivalent to the concentration and enrichment of protein and polysaccharide；
S7, the deionized water solution containing polysaccharide and protein is placed in 20 DEG C of low temperature environments below makes in solution not
The starch of removing settles, and takes after supernatant again through supercentrifuge secondary degreasing, final to be made rich in polysaccharide and protein
Semen Coicis lotion.
Wherein, it is further comprised the steps of: between step S4 and S5
S3-1: being added nonpolar macroporous adsorption resin in uniformly mixed Semen Coicis slurries, adsorbs miscellaneous in Semen Coicis slurries
After matter, filtering Semen Coicis slurries obtain filtrate；The nonpolar macroporous adsorption resin is different from general ion exchange resin, has both
Adsorptivity and screenability；Protein polar molecule and polysaccharide polarity point in nonpolar macroporous absorbent resin and Semen Coicis slurries
Adsorption effect will not be generated between son, can retaining protein and polysaccharide, while utilize " similar to mix " principle adsorb polarity
The impurity molecules such as small pigment, play the role of protein purification and polysaccharide.
Wherein, compound coixlacrymajobi powder is mixed by the raw material of following parts by weight: 200 parts of Semen Coicis lotion, jujube Aqueous extracts 100
Part, 50 parts of tea polysaccharide, 50 parts of polysaccharides, 50 parts of corn silk polysaccharide are not necessarily to external adding water；The mixed solution of the compound coixlacrymajobi powder
By vacuum concentration, vacuum drying, crushing and sterilizing, final instant compound coixlacrymajobi powder.
Embodiment 2
A kind of preparation method of Semen Coicis extract, specifically comprises the following steps:
S1, Semen Coicis particle is placed in drying in drying box, drying temperature is 70 DEG C；The heart of a lotus seed after drying is crushed using pulverizer
Rice grain, and 220 mesh screens are crossed, obtain dry coixlacrymajobi powder；Select 220 mesh screens that can guarantee that the partial size of coixlacrymajobi powder is 0.06
Between~0.07mm, the smaller active constituent that can guarantee in Semen Coicis of the partial size of Semen Coicis can be dissolved out more effectively, if partial size
It is too small, phenomena such as will appear floating powder and extract, obtain dry coixlacrymajobi powder；
S2, it is directly added into edible organic acid extraction in coixlacrymajobi powder, handles the starch in removal coixlacrymajobi powder using acid；It is described
Edible organic acid tartaric acid；The acid processing time is 40min, and temperature is 45 DEG C；Then, in the coixlacrymajobi powder solution after acidification
Degreasing agent is added and carries out degreasing pretreatment, the degreasing agent is molten for the mixing of alkali metal hydroxide and alkali metal hydrogencarbonate
Liquid；Desizing and lipid are removed with membrane filtration after degreasing, finally dry coixlacrymajobi powder under the conditions of 70 DEG C；Semen Coicis slurries after acidification
It is reacted with degreasing agent, pigment and amine in Semen Coicis slurries can be made to separate in a salt form, accelerate degreasing speed, improved
Degreasing efficiency；
S3, in degreasing and except being added deionized water in the coixlacrymajobi powder after starch, solid-liquid ratio 1:60, the heart of a lotus seed that will be uniformly mixed
Rice & peanut milk liquid is placed in Microfluidizer to be handled by dynamic super-voltage micro jet, the processing pressure of the super-voltage micro jet processing
Power is 160Mpa, and treatment temperature is 90 DEG C, and number of processes is 3 times, is handled by super-voltage micro jet, and then promote in coixlacrymajobi powder
The dissolution of polysaccharide and protein is extracted；Dynamic super-voltage micro jet technology passes through strong shearing force, high-speed impact power and height
Frequency vibration is swung, and the function and effect of super-pressure are reached under relatively low pressure condition, is scattered in so that coixlacrymajobi powder is evengranular
In sample solution, so that Semen Coicis sample cell degree of crushing is further exacerbated by, cell resistance disappears, and intracellular effective component can
Quickly to dissolve into deionized water, be conducive to the progress extracted；
S4, deionized water is added or evaporates the deionized water in slurries, and then adjust by super-voltage micro jet processing
The concentration of Semen Coicis slurries is added ionic strength adjustor, makes itself and Semen Coicis slurries so that the mass concentration of Semen Coicis slurries is 4%
Be uniformly mixed, the ionic strength for adjusting Semen Coicis slurries is lower than 0.4M, while extract protein in sample detection Semen Coicis slurries etc.
The dissociation number pKa of electricity point PI and polysaccharide, and according to the pH of measured value adjusting Semen Coicis slurries, so that the pH of Semen Coicis slurries is greater than protein
Iso-electric point, less than the dissociation number pKa of polysaccharide；Protein and polysaccharide in Semen Coicis is soluble in water, in water due to protein
Thermodynamics is incompatible and cause mutually to separate between polysaccharide, generates two kinds of liquid-liquid separation phenomenons, i.e. discrete phase separation and association phase
Separation, when sample concentration is lower than 3~4wt%, solution system ionic strength is lower than 0.4M, and system pH is between isoelectric points of proteins
(PI) between polysaccharide dissociation number (pKa) when, two kinds of macromolecules have xenogenesis charge in solution, mutually inhale between two kinds of macromolecules
Draw to form complex coacervation object；The ionic strength adjustor is that the mixing of neutral indifferent electrolyte, buffer and screening agent is molten
Liquid；The indifferent electrolyte is the mixed solution of potassium nitrate and sodium perchlorate；The buffer is PBS buffer, for adjusting
The pH of Semen Coicis slurries, so that the pH of Semen Coicis slurries is maintained in suitable range；The screening agent is citrate and tartaric acid
The mixed solution of salt, for sheltering, interfering ion being complexed.
Organic polymer adsorbent is added in S5, Semen Coicis slurries after adjustment, to the aggregate of protein and polysaccharide into
Row absorption, is aided with the ultrasonic treatment of 40min, supersonic frequency 150w in adsorption process；The organic polymer adsorbent
The volume ratio of additive amount and Semen Coicis slurries is 1:12, and adsorption temp is 50 DEG C, sorption reaction time 2h；Protein and more at this time
The complex coacervation object of sugar can be adsorbed by organic polymer adsorbent；The organic polymer adsorbent has by component A and B component group
At the component A is chitosan derivatives, and B component is cationic liquid polymer material；The volume of the component A and B component
Than for 3:1；Chitosan derivatives are selected as the main component of organic polymer adsorbent, are because on chitosan macromolecular chain
Simultaneously containing two kinds of active reactive groups of amido and hydroxyl, different groups can be imported by various reactions, preparation is not
Same chitosan derivatives, the chitosan derivatives of formation, the group of importing can choose the base of adsorbable protein and polysaccharide
Group, while chitosan Nantural non-toxic, absorption property is good, no adsorptive selectivity, is capable of the aggregate of protein and polysaccharide simultaneously, because
This selects chitosan as the main component of organic high molecular polymer adsorbent；Select cationic liquid polymer as B group
Part due in Semen Coicis slurries pH value be consistently greater than isoelectric points of proteins；Absorption behavior of the protein on ion liquid polymer with
The net charge relationship of protein is little, the surface static electricity lotus property mainly contacted with protein with ion liquid polymer
Matter, number are related；Protein surface not only carries positive (or negative) charge, but carries positive and negative charge simultaneously, only positive (or
It is negative) amount of charge is opposite is more than negative (or just) charge, simultaneously because the inhomogeneities of protein surface distribution of charges, works as protein
Isoelectric point when being lower than the pH of Semen Coicis slurries, although protein with negative net charge, still has asymmetric positive electrostatic on molecule
Lotus distribution and cationic liquid polymer contact, therefore cationic liquid polymer can adsorb this proteinoid；
S6, it is filtered after standing the Semen Coicis slurries after adsorption reaction, collects the organic polymer adsorbent filtered out, successively use
Deionized water and containing sodium chloride solution deionized water elution polysaccharide and protein, final polysaccharide and protein be dissolved in deionization
In water, filtering and elution process are equivalent to the concentration and enrichment of protein and polysaccharide；
S7, the deionized water solution containing polysaccharide and protein is placed in 20 DEG C of low temperature environments below makes in solution not
The starch of removing settles, and takes after supernatant again through supercentrifuge secondary degreasing, final to be made rich in polysaccharide and protein
Semen Coicis lotion.
Wherein, it is further comprised the steps of: between step S4 and S5
S3-1: being added nonpolar macroporous adsorption resin in uniformly mixed Semen Coicis slurries, adsorbs miscellaneous in Semen Coicis slurries
After matter, filtering Semen Coicis slurries obtain filtrate；The nonpolar macroporous adsorption resin is different from general ion exchange resin, has both
Adsorptivity and screenability；Protein polar molecule and polysaccharide polarity point in nonpolar macroporous absorbent resin and Semen Coicis slurries
Adsorption effect will not be generated between son, can retaining protein and polysaccharide, while utilize " similar to mix " principle adsorb polarity
The impurity molecules such as small pigment, play the role of protein purification and polysaccharide.
Wherein, compound coixlacrymajobi powder is mixed by the raw material of following parts by weight: 400 parts of Semen Coicis lotion, jujube Aqueous extracts 200
Part, 70 parts of tea polysaccharide, 70 parts of polysaccharides, 70 parts of corn silk polysaccharide are not necessarily to external adding water；The mixed solution of the compound coixlacrymajobi powder
By vacuum concentration, vacuum drying, crushing and sterilizing, final instant compound coixlacrymajobi powder.
Embodiment 3
A kind of preparation method of Semen Coicis extract, specifically comprises the following steps:
S1, Semen Coicis particle is placed in drying in drying box, drying temperature is 65 DEG C；The heart of a lotus seed after drying is crushed using pulverizer
Rice grain, and 220 mesh screens are crossed, obtain dry coixlacrymajobi powder；Select 220 mesh screens that can guarantee that the partial size of coixlacrymajobi powder is 0.06
Between~0.07mm, the smaller active constituent that can guarantee in Semen Coicis of the partial size of Semen Coicis can be dissolved out more effectively, if partial size
It is too small, phenomena such as will appear floating powder and extract, obtain dry coixlacrymajobi powder；
S2, it is directly added into edible organic acid extraction in coixlacrymajobi powder, handles the starch in removal coixlacrymajobi powder using acid；It is described
Edible organic acid is citric acid；The acid processing time is 35min, and temperature is 40 DEG C；Then, the coixlacrymajobi powder solution after acidification
Middle addition degreasing agent carries out degreasing pretreatment, and the degreasing agent is alkali carbonate；Desizing is removed with membrane filtration after degreasing
And lipid, coixlacrymajobi powder is finally dried under the conditions of 65 DEG C；Semen Coicis slurries after acidification are reacted with degreasing agent, can make Semen Coicis slurries
In pigment and amine separate in a salt form, accelerate degreasing speed, improve degreasing efficiency；
S3, in degreasing and except being added deionized water in the coixlacrymajobi powder after starch, solid-liquid ratio 1:50, the heart of a lotus seed that will be uniformly mixed
Rice & peanut milk liquid is placed in Microfluidizer to be handled by dynamic super-voltage micro jet, the processing pressure of the super-voltage micro jet processing
Power is 100Mpa, and treatment temperature is 85 DEG C, and number of processes is 2 times, is handled by super-voltage micro jet, and then promote in coixlacrymajobi powder
The dissolution of polysaccharide and protein is extracted；Dynamic super-voltage micro jet technology passes through strong shearing force, high-speed impact power and height
Frequency vibration is swung, and the function and effect of super-pressure are reached under relatively low pressure condition, is scattered in so that coixlacrymajobi powder is evengranular
In sample solution, so that Semen Coicis sample cell degree of crushing is further exacerbated by, cell resistance disappears, and intracellular effective component can
Quickly to dissolve into deionized water, be conducive to the progress extracted；
S4, deionized water is added or evaporates the deionized water in slurries, and then adjust by super-voltage micro jet processing
The concentration of Semen Coicis slurries is added ionic strength adjustor, makes itself and Semen Coicis slurries so that the mass concentration of Semen Coicis slurries is 3%
It is uniformly mixed；The ionic strength for adjusting Semen Coicis slurries is lower than 0.4M, at the same extract protein in sample detection Semen Coicis slurries etc.
The dissociation number pKa of electricity point PI and polysaccharide, and according to the pH of measured value adjusting Semen Coicis slurries, so that the pH of Semen Coicis slurries is greater than protein
Iso-electric point, less than the dissociation number pKa of polysaccharide；Protein and polysaccharide in Semen Coicis is soluble in water, in water due to protein
Thermodynamics is incompatible and cause mutually to separate between polysaccharide, generates two kinds of liquid-liquid separation phenomenons, i.e. discrete phase separation and association phase
Separation, when sample concentration is lower than 3~4wt%, solution system ionic strength is lower than 0.4M, and system pH is between isoelectric points of proteins
(PI) between polysaccharide dissociation number (pKa) when, two kinds of macromolecules have xenogenesis charge in solution, mutually inhale between two kinds of macromolecules
Draw to form complex coacervation object；The ionic strength adjustor is that the mixing of neutral indifferent electrolyte, buffer and screening agent is molten
Liquid；The indifferent electrolyte is sodium perchlorate；The buffer is Acetic acid-sodium acetate buffer, for adjusting Semen Coicis slurries
PH, so that the pH of Semen Coicis slurries is maintained in suitable range；The screening agent is the mixed solution of citrate and acetate,
For sheltering, interfering ion being complexed；
Organic polymer adsorbent is added in S5, Semen Coicis slurries after adjustment, to the aggregate of protein and polysaccharide into
Row absorption, is aided with the ultrasonic treatment of 35min, supersonic frequency 120w in adsorption process；The organic polymer adsorbent
The volume ratio of additive amount and Semen Coicis slurries is 1:11, and adsorption temp is 45 DEG C, sorption reaction time 1.5h；At this time protein and
The complex coacervation object of polysaccharide can be adsorbed by organic polymer adsorbent；The organic polymer adsorbent has by component A and B component
Composition, the component A are chitosan derivatives, and B component is in cation exchange resin；The volume ratio of the component A and B component
For 3:1；Chitosan derivatives are selected as the main component of organic polymer adsorbent, are because same on chitosan macromolecular chain
Two kinds of active reactive groups of Shi Hanyou amido and hydroxyl can import different groups by various reactions, and preparation is different
Chitosan derivatives, the chitosan derivatives of formation, the group of importing can choose the group of adsorbable protein and polysaccharide,
Simultaneously chitosan Nantural non-toxic, absorption property is good, no adsorptive selectivity, can simultaneously protein and polysaccharide aggregate, therefore
Select chitosan as the main component of organic high molecular polymer adsorbent；Selection cation exchange resin is since Semen Coicis is starched
PH value is consistently greater than isoelectric points of proteins in liquid；The net charge of absorption behavior and protein of the protein on ion exchange resin
Relationship is little, mainly related with surface static electricity lotus property, the number of contacts ionic exchange resin with protein；Protein
Surface not only carries positive (or negative) charge, but carries positive and negative charge simultaneously, and only positive (or negative) amount of charge is opposite to be more than
Negative (or just) charge, simultaneously because the inhomogeneities of protein surface distribution of charges, when the isoelectric point of protein is starched lower than Semen Coicis
When the pH of liquid, although protein with negative net charge, still has asymmetric positive electrostatic charge distribution to exchange with cationic on molecule
Resin contact, therefore cation exchange resin can adsorb this proteinoid；
S6, it is filtered after standing the Semen Coicis slurries after adsorption reaction, collects the organic polymer adsorbent filtered out, successively use
Deionized water and containing sodium chloride solution deionized water elution polysaccharide and protein, final polysaccharide and protein be dissolved in deionization
In water, filtering and elution process are equivalent to the concentration and enrichment of protein and polysaccharide；
S7, the deionized water solution containing polysaccharide and protein is placed in 20 DEG C of low temperature environments below makes in solution not
The starch of removing settles, and takes after supernatant again through supercentrifuge secondary degreasing, final to be made rich in polysaccharide and protein
Semen Coicis lotion.
Wherein, it is further comprised the steps of: between step S4 and S5
S3-1: being added nonpolar macroporous adsorption resin in uniformly mixed Semen Coicis slurries, adsorbs miscellaneous in Semen Coicis slurries
After matter, filtering Semen Coicis slurries obtain filtrate；The nonpolar macroporous adsorption resin is different from general ion exchange resin, has both
Adsorptivity and screenability；Protein polar molecule and polysaccharide polarity point in nonpolar macroporous absorbent resin and Semen Coicis slurries
Adsorption effect will not be generated between son, can retaining protein and polysaccharide, while utilize " similar to mix " principle adsorb polarity
The impurity molecules such as small pigment, play the role of protein purification and polysaccharide.
Wherein, compound coixlacrymajobi powder is mixed by the raw material of following parts by weight: 300 parts of Semen Coicis lotion, jujube Aqueous extracts 180
Part, 60 parts of tea polysaccharide, 60 parts of polysaccharides, 60 parts of corn silk polysaccharide are not necessarily to external adding water；The mixed solution of the compound coixlacrymajobi powder
By vacuum concentration, vacuum drying, crushing and sterilizing, final instant compound coixlacrymajobi powder.
In conclusion embodiment 1 is into embodiment 3, the preparation method of the organic polymer adsorbent specifically include with
Lower step:
A1, chitosan or sodium alginate are dissolved with acetic acid solution, uniform dispersion liquid is made, in chitosan or sodium alginate
It is added in dispersion liquid, chitosan microball is prepared using emulsion-crosslinking method；
A2, chitosan microball is put into plasma activation instrument, under conditions of pressure is 40~80Pa, with plasma two
Carbon oxide gas handles 300~500s, the chitosan microball after being activated；
A3, using chitosan microball obtained in A2 as kernel, after surface grafting polymerization reacts, acquisition surface is had
The organic polymer adsorbent of macromolecule cation exchanger resin.
Wherein, for embodiment 1 into embodiment 3, the organic polymer adsorbent after eluting protein and polysaccharide can pass through electricity
The method of solution and combination of ultrasound carries out organic polymer adsorbent reactivation, by electrolysis so that the realization of organic polymer adsorbent is de-
Attached regeneration；The organic polymer adsorbent is put into electrolytic cell, using Fe, Ti, Al, Pd, Cu, Zn, Sn, Cu/Zn,
The electrode of Cu/Pd or Ti/Pd is as cathode, and using including Pt, Ir, Ru, the insoluble electrode of Pd or Pb system is as anode, use
Organic polymer adsorbent is put into containing in sodium chloride solution as electrolyte by sodium chloride solution, current density 10~
50mA/cm2Under, it is electrolysed 1~3h；It is aided with ultrasonic treatment while electrolysis, under the auxiliary of the high-frequency vibration of ultrasound, shortens and inhale
Attached dose of reproduction time and raising adsorbent reactivation efficiency regenerate efficiency of post treatment every time and are only reduced to 2~10%, and realizes multiple
It recycles and reuses effect and reach best, generate preferable environmental benefit.
Wherein, the step of protein and polysaccharide elute:
B1, the organic polymer adsorbent and deionized water for having adsorbed protein and polysaccharide aggregate being collected by filtration are set
In centrifuge container, deionized water additional amount is 2~4 times of organic polymer adsorbent, stands 12 under conditions of 4~10 DEG C
~be centrifugated afterwards for 24 hours, centrifuge separation revolving speed is 2000~3000r/min, and centrifugation time is 10~15min；
B2, sodium chloride solution is added into centrifuge container, so that the concentration of sodium-chloride water solution is 1mol/L~1.5mol/
L, stand 12 under conditions of 4~10 DEG C~it is centrifugated afterwards for 24 hours, centrifuge separation revolving speed is 2000~3000r/min, when centrifugation
Between be 10~15min；
B3, the resulting sediment of centrifuge separation is collected, is passed into the heart of a lotus seed for being concentrated by evaporation and both obtaining and being rich in protein and polysaccharide
Rice milk liquid.
Comparative example 1:
Semen Coicis is crushed, then smashed Semen Coicis is mixed with the compound enzyme aqueous solution of EM；Mixed liquor ultrasonication with
And under heated condition, mixed liquor pH value is adjusted, ferments, ultimately forms fermentation mixed liquor；Fermentation mixed liquor is filtered,
Taking precipitate；Freeze-day with constant temperature in far infrared constant temperature drying box is put the precipitate in, Semen Coicis albumen is finally obtained, using this enzymatic isolation method
The Semen Coicis albumen of acquisition, output capacity, that is, yield are 62.67%；
Comparative example 2:
Semen Coicis is crushed, successively through water mention polysaccharide, alkali carries protein and etc. the continuous polysaccharide and albumen extracted in Semen Coicis
Matter, wherein polysaccharide yield is 8.45%, and protein yield is 8.24%.
Compare above-mentioned two embodiment, it is known that when only extracting protein, the yield of protein is higher, extracts polysaccharide when continuous
When with protein, the yield of polysaccharide and protein declines in pairs, and only 8% or so.
Protein and polyoses content detection: it is detected using CSY-SD8 protein content detector, using phenol sulfuric acid
Method detects polyoses content.Gross protein percentage composition 16.25% in Semen Coicis before extracting, the percentage composition of polysaccharide are 42.51%.
The yield of protein and polysaccharide in 1 Semen Coicis extract of table
| Embodiment 1 | Embodiment 2 | Embodiment 3 | |
| Protein yield (%) | 28.71 | 29.41 | 31.14 |
| Polysaccharide yield (%) | 16.16 | 15.48 | 18.12 |
The purity of protein and polysaccharide in 2 Semen Coicis extract of table
| Embodiment 1 | Embodiment 2 | Embodiment 3 | |
| Protein extract purity (%) | 68.22 | 67.34 | 72.17 |
| Polyoses extract purity (%) | 74.21 | 73.67 | 75.83 |
By the data in table 1 compared with comparative example 1, comparative example 2, it is known that using synchronous extraction side of the invention
Method extracts polysaccharide and protein compared to continuous, and conversion ratio is higher, and purity is higher.
Protein component is analyzed in 3 Semen Coicis extract of table
| Classification | Percentage composition (%) |
| Albumin | 2.81 |
| Globulin | 8.12 |
| Alcohol soluble protein | 40.53 |
| Glutelin | 32.78 |
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included in protection scope of the present invention.
Claims (9)
1. a kind of preparation method of Semen Coicis extract, it is characterised in that: specifically comprise the following steps:
S1, Semen Coicis particle is placed in drying in drying box, drying temperature is 60 ~ 70 DEG C；The heart of a lotus seed after drying is crushed using pulverizer
Rice grain, and 220 mesh screens are crossed, obtain dry coixlacrymajobi powder；
S2, it is directly added into edible organic acid extraction in coixlacrymajobi powder, the starch in removal coixlacrymajobi powder is handled using acid, then in acid
Degreasing agent is added in coixlacrymajobi powder solution after change and carries out degreasing pretreatment, removes desizing and lipid with membrane filtration, finally 60 ~
Dry coixlacrymajobi powder under the conditions of 70 DEG C；
S3, deionized water is added in except the drying coixlacrymajobi powder after starch and degreasing, solid-liquid ratio is 1:40 ~ 60, will be uniformly mixed
Semen Coicis slurries be placed in Microfluidizer by dynamic super-voltage micro jet handle, and then promote Semen Coicis slurries in polysaccharide and
The dissolution of protein is extracted；
S4, the deionized water by being added in deionized water or evaporation Semen Coicis slurries, and then adjust and penetrated by dynamic super-voltage is micro-
The concentration of the Semen Coicis slurries of stream process so that the mass concentration of Semen Coicis slurries be 2 ~ 4%, be added ionic strength adjustor, make its with
Semen Coicis slurries are uniformly mixed, and the ionic strength for adjusting Semen Coicis slurries is lower than 0.4M, while extracting egg in sample detection Semen Coicis slurries
The iso-electric point of white matter and the dissociation number pKa of polysaccharide, and according to the pH of measured value adjusting Semen Coicis slurries, keep the pH of Semen Coicis slurries big
In the iso-electric point of protein, less than the dissociation number pKa of polysaccharide, promote protein and polysaccharide agglomerates into aggregate；
Organic polymer adsorbent is added in S5, Semen Coicis slurries after adjustment, and the aggregate of protein and polysaccharide is inhaled
It is attached, the ultrasonic treatment of 30 ~ 40min is aided in adsorption process, supersonic frequency is 100 ~ 150w；The organic polymer adsorbent
Additive amount and Semen Coicis slurries volume ratio be 1:10 ~ 12, adsorption temp be 40 ~ 50 DEG C, sorption reaction time be 1 ~ 2h；
S6, will Semen Coicis slurries after adsorption reaction stand after filter, collect the organic polymer adsorbent filtered out, successively spend from
Sub- water and containing sodium chloride solution deionized water elution polysaccharide and protein, the polysaccharide and protein finally eluted be dissolved in
In deionized water；Filtering and elution process are equivalent to the concentration and enrichment of protein and polysaccharide；
S7, the deionized water solution containing polysaccharide and protein is placed in 20 DEG C of low temperature environments below makes not remove in solution
Starch sedimentation, take again by supercentrifuge secondary degreasing after supernatant, it is final that the Semen Coicis rich in polysaccharide and protein is made
Lotion.
2. the preparation method of Semen Coicis extract according to claim 1, it is characterised in that: further include between step S3 and S4
Step:
S3-1: being added macroporous absorbent resin in uniformly mixed Semen Coicis slurries, after adsorbing the impurity in Semen Coicis slurries, filters the heart of a lotus seed
Rice & peanut milk liquid obtains filtrate.
3. the preparation method of Semen Coicis extract according to claim 2, it is characterised in that: eaten described in step S2 organic
Acid is one of citric acid or tartaric acid；The acid processing time is 30 ~ 40min, and temperature is 35 ~ 45 DEG C；The degreasing agent
For one of alkali metal hydroxide, alkali carbonate or alkali metal hydrogencarbonate or a variety of.
4. the preparation method of Semen Coicis extract according to claim 2, it is characterised in that；Dynamic ultrahigh described in step S3
The processing pressure for pressing microjet processing is 60 ~ 160Mpa, and treatment temperature is 80 ~ 90 DEG C, and number of processes is 1 ~ 3 time.
5. the preparation method of Semen Coicis extract according to claim 2, it is characterised in that: ionic strength described in step S4
Regulator is the mixed solution of neutral indifferent electrolyte, buffer and screening agent；The indifferent electrolyte is sodium chloride, nitric acid
One of potassium or sodium perchlorate are a variety of；The buffer be one of Acetic acid-sodium acetate buffer or PBS buffer,
For adjusting the pH of Semen Coicis slurries；The screening agent is one of citrate, acetate or tartrate or a variety of.
6. the preparation method of Semen Coicis extract according to claim 2, it is characterised in that: the organic polymer in step S5
Adsorbent is made of component A and B component, one of the component A chitosan or sodium alginate；The B component is cation
One of liquid polymer material or cation exchange resin；The volume ratio of the component A and B component is 3:1.
7. the preparation method of Semen Coicis extract according to claim 2, it is characterised in that: the organic polymer adsorbent
Preparation method specifically includes the following steps:
A1, chitosan or sodium alginate are dissolved with acetic acid solution, uniform dispersion liquid is made, dispersed in chitosan or sodium alginate
It is added in liquid, chitosan microball is prepared using emulsion-crosslinking method；
A2, chitosan microball is put into plasma activation instrument, under conditions of pressure is 40~80Pa, with plasma titanium dioxide
Carbon 300~500s of gas treatment, the chitosan microball after being activated；
A3, using chitosan microball obtained in A2 as kernel, after surface grafting polymerization reacts, obtain surface with high score
The organic polymer adsorbent of sub- cation exchange resin.
8. the preparation method of Semen Coicis extract according to claim 2, it is characterised in that: after elution protein and polysaccharide
Organic polymer adsorbent carries out the adsorbent reactivation of organic polymer by the method for electrolysis and ultrasound, realizes following for adsorbent
Ring utilizes.
9. a kind of be applied to compound coixlacrymajobi powder to Semen Coicis extract made from 8 any preparation methods according to claim 1
Compounding, it is characterised in that: compound coixlacrymajobi powder is mixed by the raw material of following parts by weight: 200-400 parts of Semen Coicis lotion, jujube water
100-200 parts of extract, 50-70 parts of tea polysaccharide, 50-70 parts of polysaccharides, 50-70 parts of corn silk polysaccharide are not necessarily to external adding water；It is described
The mixed solution of compound coixlacrymajobi powder is by vacuum concentration, vacuum drying, crushing and sterilizing, final instant compound coixlacrymajobi powder.
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| CN110237013A (en) * | 2019-07-01 | 2019-09-17 | 上海欧润化妆品有限公司 | A kind of extracting method of Semen Coicis |
| CN111150708A (en) * | 2019-12-31 | 2020-05-15 | 浙江工商大学 | Method for synchronously preparing functional homologous nano composition and application |
| CN111150708B (en) * | 2019-12-31 | 2022-04-08 | 浙江工商大学 | Method for synchronously preparing functional homologous nano composition and application |
| CN112690388A (en) * | 2020-12-28 | 2021-04-23 | 广西壮族自治区农业科学院 | Preparation method of polygonatum sibiricum functional lactobacillus beverage |
| CN114931556A (en) * | 2022-06-15 | 2022-08-23 | 劲牌持正堂药业有限公司 | Preparation method of cattail pollen charcoal formula granules |
| CN114931556B (en) * | 2022-06-15 | 2023-07-25 | 劲牌持正堂药业有限公司 | Preparation method of cattail pollen charcoal formula particles |
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Application publication date: 20190111 |