CN114931556A - Preparation method of cattail pollen charcoal formula granules - Google Patents
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Abstract
The invention discloses a preparation method of cattail pollen carbon formula particles, which specifically comprises the following steps: (1) decocting; (2) primary impurity removal: (3) centrifuging; (4) dynamic high-pressure micro-jet treatment; (5) and (5) granulating. The prepared formula particle overcomes the defects of high paste yield and low content of effective components of decoction pieces in the preparation process, and effectively improves the dissolution stability of the cattail pollen carbon formula particle in water. The cattail pollen carbon formula particle prepared by the invention has the characteristic of instant solubility, and the uniformity degree of active ingredients in the cattail pollen carbon formula particle is strong.
Description
Technical Field
The invention relates to the technical field of traditional Chinese medicine granule preparation, and in particular relates to a preparation method of cattail pollen charcoal formula granules.
Background
The pollen Typhae charcoal is processed product obtained by parching dried pollen of Typha angustifolia L of Typhaceae. Cattail pollen is sweet in nature and mild in nature, mainly comprises typha sterol, isorhamnetin, fatty oil, yellow pigment and the like, and has the effects of stopping bleeding, removing blood stasis and treating stranguria. Modern medical research shows that the cattail pollen charcoal has an inhibitory effect on in vitro tumor cells. The pollen Typhae charcoal water extract or 5% ethanol extract can obviously shorten the blood coagulation time of rabbits, and the pollen Typhae charcoal powder can be decocted for treating blood loss.
The dynamic high-pressure micro-jet technology is an environment-friendly continuous physical modification method, and is a modern novel processing technology which integrates multiple unit operations such as conveying, mixing, high-pressure, high-speed impact, shearing and the like, the particle size of a material is dispersed to the nanometer level, and the particle size distribution is uniform after homogenization. The method has wide application range, and can be used for homogenizing and dispersing emulsion, dispersion liquid, suspension, etc. in various industries, especially for research and preparation of nano-drug preparation.
The traditional Chinese medicine prescription granule is a novel prescription medicine with uniform specification, uniform dosage and uniform quality standard, which is prepared by taking traditional Chinese medicine decoction pieces as raw materials and processing the traditional Chinese medicine decoction pieces through production processes of extraction, separation, concentration, drying, granulation, packaging and the like. The traditional Chinese medicine formula particle can replace traditional decoction pieces for clinical syndrome differentiation treatment of traditional Chinese medical technicians, increases and decreases the demand according to the symptoms, and has the characteristics of convenience in carrying, small administration dosage, safety, stability and the like. At present, the pollen typhae charcoal formula particles have the conditions of overhigh cream yield, overlow content of effective components and uneven distribution in the particles in the production process. Therefore, controlling the cream yield of the cattail pollen charcoal medicinal decoction pieces and stabilizing the content and uniformity of the active ingredients in the particles become important means for ensuring the effective clinical application of the cattail pollen charcoal formula particles. In addition, the traditional cattail pollen charcoal formula particles have obvious precipitation and layering phenomena after being dissolved in pure water, the taking experience of patients is influenced, and the improvement of the dissolution stability of the product also becomes one of the problems to be solved urgently.
Disclosure of Invention
The invention aims to provide a preparation method of cattail pollen carbon formula particles, and aims to solve the technical problems.
The technical scheme provided by the invention is as follows:
a preparation method of cattail pollen charcoal formula particles comprises the following steps:
step one, decocting: firstly, weighing an appropriate amount of cattail pollen charcoal medicinal material decoction pieces, wrapping the cattail pollen charcoal medicinal material decoction pieces with a non-woven cloth bag in a double-layer mode, adding pure water which is 5-20 times of the medicinal material in weight, soaking for 30-60min before the first decoction, decocting for 2 times, and decocting for 20-90min each time, and combining decoction liquids;
step two, primary impurity removal: standing the decoction at 4-15 deg.C for 24-96 hr, collecting the upper layer liquid, and removing the lower layer precipitate;
step three, centrifugation: enabling the upper layer liquid obtained in the step two to pass through a centrifugal machine, wherein the centrifugal rotating speed is 4500-;
step four, dynamic high-pressure micro-jet treatment: performing high-pressure microjet treatment on the filtrate obtained in the third step for 5-25 times under the treatment pressure of 50-180MPa, collecting the treated liquid, concentrating, and drying to obtain pollen Typhae charcoal dry extract powder;
step five, granulating: adding medicinal maltodextrin, dextrin or soluble starch with the mass of 30-75% of the mass of the dry paste powder into the obtained dry paste powder, granulating, drying and grading to obtain the cattail pollen charcoal formula granules.
Preferably, in the first step, the decoction conditions are 2 times of decoction, each time of decoction lasts for 60min, the ratio of decoction pieces of the medicinal materials to pure water for each decoction is 1:14, and the decoction pieces are soaked for 40min in advance before the first decoction.
Preferably, in the second step, the standing temperature is 10 ℃ and the standing time is 24 hours.
Preferably, in the third step, the centrifugal rotating speed is 5500r/min, and the centrifugal time is 10 min.
Preferably, in the third step, the mesh number of the filter bag is 200 meshes.
Preferably, in the fourth step, the dynamic high-pressure micro-jet treatment pressure is 100MPa, and the treatment times are 10 times.
Preferably, in the fifth step, the auxiliary materials are medicinal maltodextrin and soluble starch, and the total addition amount of the medicinal maltodextrin and the soluble starch is 69% of the mass of the cattail pollen charcoal dry paste powder.
Preferably, in the fifth step, the cream yield of the cattail pollen charcoal decoction pieces is 8.0%, the extract is 18.4%, and every 1g of cattail pollen charcoal formula granules contains typhin (C) 34 H 42 O 20 ) And isorhamnetin-3-O-neohesperidin (C) 28 H 32 O 16 ) The total amount was 6.6 mg.
The invention has the following beneficial effects:
1. compared with the traditional mode of directly decocting decoction pieces, the cattail pollen carbon formula particle prepared by the invention has the characteristics of instant use, small volume, clear dosage and convenient application and carrying, is convenient for a traditional Chinese medical specialist to use in clinical diagnosis and treatment, and ensures the consistency of dosage each time in clinical treatment.
2. The method removes impurities in the cattail pollen charcoal decoction liquid by means of preliminary standing for impurity removal, centrifugation and filter bag penetration, reduces the paste yield of decoction pieces, and improves the content of effective components in formula granules.
3. The invention utilizes the dynamic high-pressure micro-jet technology to ensure that the content of the effective components in the formula particles is uniform, and the stability of the formula particles can be kept for a long time after the formula particles are quickly dissolved in pure water at normal temperature.
Drawings
FIG. 1 is a reference substance characteristic spectrum of pollen Typhae preparata;
FIG. 2 is a characteristic spectrum of the cattail pollen charcoal granules in each example and comparative example;
FIG. 3 is a liquid chromatogram of a control solution;
FIG. 4 is a liquid chromatogram of cattail pollen charcoal formulation granule in each example;
FIG. 5 is a liquid chromatogram of the cattail pollen charcoal formulation granule in each comparative example.
Detailed Description
Example 1
The embodiment discloses a preparation method of cattail pollen carbon formula particles, which comprises the following steps:
(1) firstly, 7300g of cattail pollen charcoal decoction piece medicinal materials are weighed, the medicinal materials are wrapped by a non-woven cloth bag in a double-layer mode, pure water 12 times of the medicinal materials in mass is added, soaking is carried out for 30min before the first decoction, the decoction is carried out for 2 times, each time, the decoction is carried out for 60min, and decoction liquids are combined.
(2) Standing the decoction at 10 deg.C for 24 hr, collecting the upper layer liquid, and removing the lower layer precipitate. And then the obtained upper layer liquid passes through a centrifuge, the centrifugal speed is 4500r/min, the centrifugal time is 10min, the centrifuged liquid is collected and passes through a 300-mesh filter bag to obtain filtrate.
(3) Performing high-pressure microjet treatment on the obtained filtrate for 10 times under the treatment pressure of 100MPa, collecting the treated liquid, and drying to obtain pollen Typhae charcoal dry extract powder. Finally, medicinal maltodextrin with the mass of 25 percent of that of the dry paste powder and soluble starch with the mass of 44 percent of that of the dry paste powder are added into the obtained dry paste powder, and the cattail pollen charcoal formula particles are obtained through granulation, drying and granule finishing.
Example 2
The embodiment discloses a preparation method of cattail pollen carbon formula particles, which comprises the following steps:
(1) firstly weighing 7300g of cattail pollen charcoal decoction pieces, wrapping with a non-woven cloth bag, adding pure water 10 times of the weight of the medicinal materials, soaking for 30min before the first decoction, decocting for 2 times, decocting for 65min each time, and combining the decoctions.
(2) Standing the decoction at 4 deg.C for 36 hr, collecting the upper layer liquid, and removing the lower layer precipitate. And then passing the obtained upper layer liquid through a centrifuge at the centrifugal rotation speed of 6500r/min for 12min, collecting the centrifuged liquid, and passing the centrifuged liquid through a 300-mesh filter bag to obtain filtrate.
(3) Performing high-pressure microjet treatment on the obtained filtrate for 10 times under the treatment pressure of 150MPa, collecting the treated liquid, and drying to obtain pollen Typhae charcoal dry extract powder. And finally, adding medicinal maltodextrin and soluble starch which account for 35 percent and 30 percent of the mass of the dry paste powder into the obtained dry paste powder, and granulating, drying and granulating to obtain the cattail pollen carbon formula particles.
Example 3
The embodiment discloses a preparation method of cattail pollen carbon formula particles, which comprises the following steps:
(1) firstly weighing 7300g of cattail pollen charcoal decoction pieces, wrapping with a non-woven cloth bag in a double layer manner, adding pure water 15 times of the weight of the medicinal materials, soaking for 30min before the first decoction, decocting for 2 times, decocting for 50min each time, and combining the decoction.
(2) Standing the decoction at 8 deg.C for 48 hr, collecting the upper layer liquid, and removing the lower layer precipitate. And then the obtained upper layer liquid passes through a centrifuge, the centrifugal speed is 8000r/min, the centrifugal time is 15min, the centrifuged liquid is collected and passes through a 400-mesh filter bag to obtain filtrate.
(3) Performing high-pressure microjet treatment on the obtained filtrate under the condition that the treatment pressure is 50MPa for 15 times, collecting the treated liquid, and drying to obtain the cattail pollen charcoal dry paste powder. And finally, adding medicinal maltodextrin and 29 percent of soluble starch which account for 40 percent of the mass of the dry paste powder into the obtained dry paste powder, and granulating, drying and granulating to obtain the cattail pollen carbon formula particles.
Comparative example 1
In the comparative example, according to the steps of example 1, the preliminary impurity removal, centrifugation and dynamic high-pressure microjet treatment steps are removed, and the other steps are unchanged, and the specific steps are as follows:
(1) firstly, 7300g of cattail pollen charcoal decoction piece medicinal materials are weighed, the medicinal materials are wrapped by a non-woven cloth bag in a double-layer mode, pure water 12 times of the medicinal materials in mass is added, soaking is carried out for 30min before the first decoction, the decoction is carried out for 2 times, each time, the decoction is carried out for 60min, and decoction liquids are combined.
(2) Concentrating the decoction, and drying to obtain pollen Typhae charcoal dry extract powder. And finally, adding medicinal maltodextrin and soluble starch which account for 25% and 44% of the dry extract powder in mass into the obtained dry extract powder, and granulating, drying and granulating to obtain the cattail pollen carbon formula granules.
Comparative example 2
In this comparative example, the dynamic high-pressure microjet treatment step was eliminated according to the procedure of example 1, and the remaining steps were unchanged, and the specific steps were as follows:
(1) firstly weighing 7300g of cattail pollen charcoal decoction pieces, wrapping with a non-woven cloth bag, adding pure water 12 times of the weight of the medicinal materials, soaking for 30min before the first decoction, decocting for 2 times, decocting for 60min each time, and combining the decoctions.
(2) Standing the decoction at 10 deg.C for 24 hr, collecting the upper layer liquid, and removing the lower layer precipitate. And then the obtained upper layer liquid passes through a centrifuge, the centrifugal speed is 4500r/min, the centrifugal time is 10min, the centrifuged liquid is collected and passes through a 300-mesh filter bag to obtain filtrate.
(3) Directly concentrating the obtained filtrate, and drying to obtain pollen Typhae charcoal dry extract powder. And finally, adding medicinal maltodextrin and soluble starch which account for 25% and 44% of the dry extract powder in mass into the obtained dry extract powder, and granulating, drying and granulating to obtain the cattail pollen carbon formula granules.
Test examples
1. Determination of pollen Typhae decoction piece paste yield
The cream yield is (M × V)/(M × V) × 100%
Wherein M represents the mass (g) of dry extract, V represents the sampling volume (mL), M represents the initial mass (g) of decoction pieces, and V represents the volume (mL) of water decoction.
The cream yield of the cattail pollen charcoal decoction pieces in different examples and comparative examples is shown in table 1.
TABLE 1 the cream yield of cattail pollen charcoal decoction pieces in different examples and comparative examples
As can be seen from Table 1, the cream yield of the cattail pollen charcoal decoction pieces in each example is in the range of 6.9-10.7%, which meets the requirement range in the claims, and compared with example 1, comparative example 1 does not use the steps of preliminary impurity removal, centrifugation and dynamic high-pressure microjet treatment in the invention, so that the cream yield exceeds the requirement value of the cream yield range. In addition, the paste yield of comparative example 2 was not significantly changed from that of example 1, indicating that the dynamic high-pressure microjet treatment did not change the paste yield.
2. Particulate extract of cattail pollen charcoal formulation in various examples and comparative examples
The extract is measured by hot dipping method under the item of alcohol-soluble extract measurement method (2201 in the general rules of the four departments of the 2020 edition of Chinese pharmacopoeia), and ethanol is used as solvent.
TABLE 2 particulate extract of cattail pollen charcoal formulations in various examples and comparative examples
As can be seen from Table 2, the extracts of the charred pollen Typhae decoction pieces in the examples and the comparative examples are higher than 10%, and the extracts meet the scope of the claims.
3. Method for measuring content of active ingredients of cattail pollen carbon formula particles in different examples and comparative examples
3.1 chromatographic conditions and System suitability test
Octadecylsilane chemically bonded silica is used as a filler (the column length is 100mm, the inner diameter is 2.1mm, and the particle size is 1.8 mu m); acetonitrile is taken as a mobile phase A, 0.1 percent glacial acetic acid solution is taken as a mobile phase B, and gradient elution is carried out according to the specification in the following table; the flow rate was 0.3ml per minute; the column temperature is 30 ℃; the detection wavelength was 254 nm. The number of theoretical plates is not less than 5000 calculated according to isorhamnetin-3-O-neohesperidin peak.
3.2 preparation of control solutions
Accurately weighing appropriate amount of isorhamnetin-3-O-neohesperidin reference substance and typhaneoside reference substance, and adding methanol to obtain a mixed solution containing 25 μ g of isorhamnetin-3-O-neohesperidin and 25 μ g of typhaneoside per 1 ml.
3.3 preparation of test solutions
Taking a proper amount of the product, grinding, precisely weighing about 0.2g, placing into a conical flask with a plug, precisely adding 25ml of 30% methanol, sealing the plug, weighing, carrying out ultrasonic treatment (power 250W and frequency 40kHz) for 30 minutes, cooling, weighing again, supplementing the lost weight with 30% methanol, shaking up, filtering, and taking the subsequent filtrate to obtain the product.
3.4 assay method
Precisely sucking 2 μ L of each of the reference solution and the sample solution, injecting into liquid chromatograph, and measuring.
3.5 analysis of assay results
TABLE 3 relevant data for determining the content of cattail pollen charcoal formulation particles in each example and comparative example
As can be seen from the results shown in FIGS. 3-5 and Table 3, the content of the cattail pollen carbon granules in each example is within the range of 1.0mg/g to 13.0mg/g, which meets the requirement. Comparative example 1 compared with example 1, the content was much lower than the lower limit of the required value of the content range because the preliminary impurity removal, centrifugation and dynamic high-pressure microjet treatment steps in the present invention were not used. In addition, comparative example 2 has no significant change in content compared to example 1, indicating that the dynamic high pressure microjet treatment does not change the finite component content values in the particles. .
4. The particle feature spectrum situation of cattail pollen carbon formula in different examples and comparative examples
4.1 chromatographic conditions and System suitability test
Octadecylsilane chemically bonded silica is used as a filler (the column length is 100mm, the inner diameter is 2.1mm, and the particle size is 1.8 mu m); acetonitrile is taken as a mobile phase A, a 0.1% glacial acetic acid solution is taken as a mobile phase B, and gradient elution is carried out according to the specification in the following table; the flow rate was 0.3ml per minute; the column temperature is 30 ℃; the detection wavelength was 254 nm. The number of theoretical plates is not less than 5000 calculated according to isorhamnetin-3-O-neohesperidin peak.
4.2 preparation of reference solutions
Taking 0.5g of cattail pollen as a reference medicinal material, putting the cattail pollen into a conical flask with a plug, adding 25mL of water, heating and refluxing for 60 minutes, cooling, shaking up, filtering, and taking a subsequent filtrate as a reference medicinal material solution. Taking the reference substance solution under the item [ content determination ] as the reference substance solution. Then taking a proper amount of p-hydroxybenzoic acid reference substance, quercetin-3-O-neohesperidin reference substance and kaempferol-3-O-neohesperidin reference substance, and respectively adding methanol to prepare solutions each containing 10 mu g of p-hydroxybenzoic acid, 25 mu g of quercetin-3-O-neohesperidin and 25 mu g of kaempferol-3-O-neohesperidin per 1mL as reference substance solutions of the reference substances.
4.3 preparation of test solutions
Taking a proper amount of the product, grinding, taking about 0.2g, precisely weighing, placing in a conical flask with a plug, precisely adding 25ml of 30% methanol, sealing the plug, weighing, carrying out ultrasonic treatment (power 250W and frequency 40kHz) for 30 minutes, cooling, weighing again, complementing the weight loss with 30% methanol, shaking up, filtering, and taking subsequent filtrate to obtain the product.
4.4 assay
Precisely sucking 2 μ L of reference solution and sample solution respectively, injecting into liquid chromatograph, and measuring. The chromatogram of the test sample should show 8 characteristic peaks, and the retention time of the 8 characteristic peaks in the chromatogram of the reference drug should correspond to that of the reference drug. Wherein 5 peaks should correspond to the retention times of the corresponding reference peaks of the control, respectively; the peak corresponding to the typhaneoside reference is the S peak, and the relative retention time of the other characteristic peaks and the S peak is calculated, wherein the relative retention time is within +/-10% of a specified value. The specified values are: 0.68 (peak 2), 0.88 (peak 3), 0.98 (peak 5).
4.5 analysis of assay results
TABLE 4 relative retention time of the peaks of the cattail pollen charcoal formulation granule in the different examples and comparative examples
As can be seen from the figure 1, the figure 2 and the table 4, the characteristic spectrum of the cattail pollen carbon formula particles in each example and the comparative example shows 8 characteristic peaks, the retention time of the 8 characteristic peaks in the chromatogram of the reference substance of the reference medicinal material corresponds to that of the 8 characteristic peaks in the chromatogram of the reference substance of the reference medicinal material, and the relative retention time of each peak is in the range and meets the requirement.
5. Dissolution stability of cattail pollen charcoal formulation granule in different examples and comparative examples
Taking a proper amount of cattail pollen carbon formula particles in each example and comparative example respectively, dissolving the cattail pollen carbon formula particles in sterile pure water at the temperature of 20 ℃, keeping the temperature and standing the cattail pollen carbon formula particles in a sterile environment, and observing the stability of the solution at intervals.
TABLE 5 dissolution stability of cattail pollen charcoal formulation granule in each example and comparative example
As can be seen from Table 5, the pellets of the cattail pollen charcoal formulation solution did not precipitate and delaminate over time in example 1, whereas the pellets precipitated and delaminated in the comparative examples. However, the delamination phenomenon occurred later in comparative example 2 than in comparative example 1, which indicates that the primary impurity removal and centrifugation have the effect of delaying the time of the delamination phenomenon to some extent, but the problem cannot be solved fundamentally. Compared with the comparative example 2, the precipitation and delamination phenomena do not occur along with the prolonging of the time after the dynamic high-pressure micro-jet treatment, which shows that the dynamic high-pressure micro-jet treatment can obviously improve the stability of the cattail pollen carbon formula particle solution and prevent the solution from delaminating and precipitating phenomena.
Based on the data analysis result, the cattail pollen carbon formula particle prepared in the embodiment of the invention has higher content of effective components and better dissolution stability in pure water. On the other hand, the cattail pollen carbon formula particle prepared by the invention has definite dosage, is convenient to apply and carry, is convenient for a traditional Chinese medical doctor to use in clinical dialectical treatment, and can ensure the consistency of dosage every time in clinical treatment. As described above, the above embodiments are only illustrative of the preferred embodiments of the present invention, and do not include all the scope of the invention. Various changes and modifications can be made by one skilled in the art without departing from the spirit and scope of the invention, and the scope of the invention is defined by the appended claims.
Claims (9)
1. A preparation method of cattail pollen charcoal formula particles is characterized by comprising the following steps: the method comprises the following steps:
step one, decocting: firstly, pollen typhae decoction pieces are weighed, double-layer wrapping and decocting are carried out by using a non-woven fabric bag, and then pure water is added for boiling to obtain decoction.
Step two, primary impurity removal: standing the decoction at a low temperature for a period of time, collecting the upper layer liquid, and discarding the lower layer precipitate.
Step three, centrifugation: and (5) enabling the upper layer liquid obtained in the step two to pass through a centrifugal machine, collecting the centrifuged liquid, and enabling the centrifuged liquid to penetrate through a filter bag to obtain a filtrate.
Step four, dynamic high-pressure micro-jet treatment: and (4) carrying out dynamic high-pressure micro-jet treatment on the filtrate in the third step, concentrating and drying to obtain the cattail pollen charcoal dry paste powder.
Step five, granulating: adding adjuvants into the obtained dry extract powder, granulating, drying, and grading to obtain pollen Typhae charcoal granule.
2. The method for preparing the cattail pollen charcoal formula particle as claimed in claim 1, wherein the method comprises the following steps: in the first step, the decoction conditions are 1-3 times of decoction, each time of decoction lasts for 20-90min, the ratio of the decoction pieces of the medicinal materials to the pure water for each time of decoction is 1:5-20, and the decoction pieces of the medicinal materials are soaked in advance for 30-60min before the first time of decoction.
3. The method for preparing the cattail pollen charcoal formula particle of claim 1, which is characterized by comprising the following steps: in the second step, the standing temperature is 4-15 ℃, and the standing time is 24-96 h.
4. The method for preparing the cattail pollen charcoal formula particle of claim 1, which is characterized by comprising the following steps: in the third step, the centrifugal rotating speed is 4500-10000r/min, and the centrifugal time is 5-30 min.
5. The method for preparing the cattail pollen charcoal formula particle as claimed in claim 1, wherein the method comprises the following steps: in the third step, the number of the filter bags is 200-400 meshes.
6. The method for preparing the cattail pollen charcoal formula particle of claim 1, which is characterized by comprising the following steps: in the fourth step, the dynamic high-pressure micro-jet treatment pressure is 50-180MPa, and the treatment times are 5-25.
7. The method for preparing the cattail pollen charcoal formula particle as claimed in claim 1, wherein the method comprises the following steps: in the fifth step, the auxiliary materials are medicinal maltodextrin, dextrin or soluble starch, and the adding amount of the auxiliary materials is 30-75% of the mass of the cattail pollen charcoal dry paste powder.
8. The method for preparing the cattail pollen charcoal formula particle of claim 1, which is characterized by comprising the following steps: in the fifth step, the cream yield of the cattail pollen charcoal decoction pieces is 6.9-10.7 percent, the extract content of the cattail pollen charcoal formula particles is more than or equal to 10.0 percent, and every 1g of cattail pollen glycoside (C) is contained in the cattail pollen charcoal formula particles 34 H 42 O 20 ) And isorhamnetin-3-O-neohesperidin (C) 28 H 32 O 16 ) The total amount is 1.0 mg-13.0 mg.
9. The method for preparing the cattail pollen charcoal formula particle of claim 1, which is characterized by comprising the following steps: in the fifth step, the cattail pollen carbon formula particles are dissolved in pure water at the temperature of 20 ℃, and no precipitation or layering phenomenon occurs after standing for 24 hours.
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| KR20070109426A (en) * | 2006-05-11 | 2007-11-15 | 한불화장품주식회사 | A cosmetic composition containing an extract of typhae pollen |
| CN105241997A (en) * | 2015-11-10 | 2016-01-13 | 广东一方制药有限公司 | Thin-layer chromatography identification method of carbon traditional Chinese medicine formula granules |
| CN109170532A (en) * | 2018-09-10 | 2019-01-11 | 福建拓天生物科技有限公司 | A kind of preparation method and application of Semen Coicis extract |
| CN114403392A (en) * | 2022-01-26 | 2022-04-29 | 西南大学 | Preparation method of instant winter jujube powder and product thereof |
-
2022
- 2022-06-15 CN CN202210672342.2A patent/CN114931556B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20070109426A (en) * | 2006-05-11 | 2007-11-15 | 한불화장품주식회사 | A cosmetic composition containing an extract of typhae pollen |
| CN105241997A (en) * | 2015-11-10 | 2016-01-13 | 广东一方制药有限公司 | Thin-layer chromatography identification method of carbon traditional Chinese medicine formula granules |
| CN109170532A (en) * | 2018-09-10 | 2019-01-11 | 福建拓天生物科技有限公司 | A kind of preparation method and application of Semen Coicis extract |
| CN114403392A (en) * | 2022-01-26 | 2022-04-29 | 西南大学 | Preparation method of instant winter jujube powder and product thereof |
Non-Patent Citations (2)
| Title |
|---|
| SAROJ S. VARPE等: "Evaluation of ant i-inflammatory activity of Typha angustifolia pollen grains extracts in experimental animals", 《INDIAN JOURNAL OF PHARMACOLOGY》, vol. 44, no. 6, pages 788 - 791 * |
| 刘斌等: "蒲黄配方颗粒质量标准的研究", 《中成药》, vol. 25, no. 8, pages 624 - 626 * |
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