CN109170512A - The biodegradation method of vomitoxin in a kind of pair of corn germ cake - Google Patents
The biodegradation method of vomitoxin in a kind of pair of corn germ cake Download PDFInfo
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- CN109170512A CN109170512A CN201811099701.XA CN201811099701A CN109170512A CN 109170512 A CN109170512 A CN 109170512A CN 201811099701 A CN201811099701 A CN 201811099701A CN 109170512 A CN109170512 A CN 109170512A
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- koji
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/28—Removal of unwanted matter, e.g. deodorisation or detoxification using microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a kind of effective biodegradable methods for vomitoxin in whitewashing corn germ cake.Compound koji is mixed by two kinds of two kinds or a kind of 15%-25% of lactobacillus acidophilus koji and saccharomyces cerevisiae koji, aspergillus niger koji and aspergillus oryzae koji or a kind of 65%-45%, bacillus subtilis koji 20%-30%.By the inoculum concentration of 3%-5%, compound koji is accessed in whitewashing corn germ cake, after stirring 30min, adding the ammonium sulfate solution containing 3.0%-4.2% to material moisture is 42%-48%, is stirred evenly, static fermentation 4h-20h, to in bed of material 30cm, when temperature rises to 36 DEG C -38 DEG C, turn over throwing (oxygenation) and be cooled to 30 DEG C -27 DEG C, throwing is turned in stopping.When temperature rises to 36 DEG C -38 DEG C again in bed of material 30cm, carries out turning over throwing (oxygenation) again and be cooled to 30 DEG C -27 DEG C, throwing is turned in stopping.It operates repeatedly, whole process keeps 36h-48h.When material temperature no longer rapidly rises, fermentation material is turned over into throwing and is cooled to 26 DEG C -28 DEG C, seals 20h-30h after material is smooth, compacting, when having sour taste spilling in fermentation tank, fermentative degradation termination.
Description
Technical field
The present invention relates in the technical field of biodegradable grain and oil institute contaminated mold toxin, in particular to corn deep processing pair
The biodegradation technique of the polluted vomitoxin of product whitewashing corn germ cake.
Technical background
It is estimated according to FAO (Food and Agriculture Organization of the United Nation), the whole world is lost as caused by mycotoxin contamination, annual up to dry hundred million beauty of number
Member, and the harm caused by people and animals is even more to be difficult to count.In recent years, mycotoxin contamination to the negative effect of husbandry sector gradually
Recognized by people, external scientific research institution has begun a series of researchs of biodegrade development for mycotoxin.At home
Less about the relevant research report of vomitoxin degradation bacteria and detoxication enzyme, efficient degradation vomitoxin enzyme can be produced by filtering out
Bacterial strain and pass through the means such as enzyme engineering, genetic engineering obtain high efficient expression detoxication enzyme be current research direction.2015
Year, " feed industry " editorial office specially invited China Agricultural University's meter to do one with regard to the biodegradable research of domestic and international vomitoxin at professor
Summary.
Meter professor etc. is in " feed industry " the 10th phase total 487th phase " vomitoxin biodegrade research of volume 36 in 2015
Progress " it talks about in a text:
Sickle-like bacteria generates a large amount of vomitoxins (DON), Polluted grains and grain.DON can cause humans and animals to be poisoned, symptom
Vomiting, hemorrhage of digestive tract, inflammation are shown as, or even dead.Currently without effective detoxicating method, adsorbent is almost without work
With；Physics and chemical method effect are unstable, destroy nutritional ingredient and the sense of taste.It is biodegradable DON high specificity, high-efficient, right
Food nourishment composition without influence, toxin can be converted to nontoxic metabolite.Existing article report degradation feed DON's is single
Bacterial strain is considerably less, more not about the application study of efficacy.The vomitoxin positive detects in Chinese mixed feed sample
Rate is up to 100%, average content 0.6mg/kg.It can be strong to humans and animals when DON content is more than 1mg/kg in feed or grain
Health generates harm, and therefore, Food and Drug Administration provides that DON limit standard is 1mg/kg, the Ministry of Agriculture of China in food
1mg/kg, which is also not to be exceeded, in value is provided to the limit standard of DON in animal feed.But up to the present, it does not close
It is reported in the research of vomitoxin degrading enzyme, also without related the reporting using microorganism or its enzyme generated research and development feed addictive
Road.Therefore, the bacterial strain-for finding new efficient degradation feed DON is the Research foundation for developing DON degradation bacteria feed addictive.
China's nonruminant complete diet pellet and the fine fodder of ruminant, are substantially dregs of beans corn type.Due to price factor,
Become the first choice for reducing feed cost in feed using corn deep processing byproduct substitution part corn-soybean meal.However corn adds deeply
During work, the one third of corn amount is byproduct, since mycotoxin all stays on corn deep processing byproduct substantially, institute
Mycotoxin is enriched with corn deep processing byproduct three times.Mycotoxin inherently exceeded corn is substituted by this product
Dregs of beans, it is necessary to take strong detoxification means.Present invention efficiently solves corn deep processing byproduct-whitewashing maize germs
The degradation problem of vomitoxin in the dregs of rice.
Summary of the invention
The technical problems to be solved by the invention: it is effective biology drop for vomitoxin in whitewashing corn germ cake
Solution reaches the detoxification purpose to whitewashing corn germ cake.Especially for " C12, the C13- epoxy in vomitoxin molecular structure
Target position of the structure (having virose primary structure basis) " as biodegrade, " the feed addictive kind announced from the Ministry of Agriculture
Catalogue " in, it screens Relative Fungi and bacterial species, by mixed fermentation technology, reaches C12 and C13 epoxy through scientific matching
Structural degradation is the purpose that C9 and C12 forms double bond group (toxicity is effectively passivated).
The technical problems to be solved by the invention can be achieved through the following technical solutions:
One, utilizes " microbial fermentation antibiotic-free feed and its production of my Patent No. ZL 201010148491.6
Method " inner disclosed lactobacillus acidophilus fermentation material production method, fermentation of bacillus subtilis material production method, saccharomyces cerevisiae hair
Ferment material production method, fermentation of Aspergillus niger material production method, aspergillus oryzae fermentation material production method, and Patent No. in person
" self-supporting repeating transmission keyboard " disclosed in ZL201220624896.7, makes lactobacillus acidophilus koji, and bacillus subtilis koji is made
Brewer yeast koji, aspergillus niger koji, aspergillus oryzae koji are spare.
Two, are by two kinds or a kind of 15%-25% of lactobacillus acidophilus koji and saccharomyces cerevisiae koji, aspergillus niger koji and rice
Two kinds of aspergillus koji or a kind of 65%-45%, it is spare that bacillus subtilis koji 20%-30% is mixed into compound koji.
Three, press the inoculum concentration of 3%-5%, and compound koji is accessed in whitewashing corn germ cake, after stirring 30min, adds and contains
The ammonium sulfate solution of 3.0%-4.2% to material moisture is 42%-48%, is sufficiently stirred.The material cloth stirred evenly
Enter fermentation tank, thickness of feed layer 80cm-120cm, (workshop is minimum need to keep room temperature at 16 DEG C or more), static fermentation 4h-
20h, to when temperature rises to 36 DEG C -38 DEG C, recycle my 201410647232.6 institute of Patent No. ZL in bed of material 30cm
Disclosed " biofermentation slot turn over throw push-pull device at fixed " carries out turning over throwings (oxygenation) being cooled to 30 DEG C -27 DEG C, stops turning over throwing.Work as the bed of material
When temperature rises to 36 DEG C -38 DEG C again in 30cm, carries out turning over throwing (oxygenation) again and be cooled to 30 DEG C -27 DEG C, throwing is turned in stopping.Instead
Multiple operation, whole process keep 36h-48h.
Four, turn over throwing cooling when material temperature no longer rapidly rises (fermentation process goes to stationary phase by logarithmic phase), by fermentation material
To 26 DEG C -28 DEG C, operation biofermentation slot turns over throwing push-pull device at fixed, material is smooth, compacting.Then 20h-30h, fermentation tank are sealed
In have sour taste spilling when, fermentative degradation termination.
Specific embodiment
One, utilizes " microbial fermentation antibiotic-free feed and its production of my Patent No. ZL 201010148491.6
Method " inner disclosed lactobacillus acidophilus fermentation material production method, fermentation of bacillus subtilis material production method, saccharomyces cerevisiae hair
Ferment material production method, fermentation of Aspergillus niger material production method, aspergillus oryzae fermentation material production method, and Patent No. ZL2012 2 in person
" self-supporting repeating transmission keyboard " disclosed in 0624896.7 makes lactobacillus acidophilus koji, bacillus subtilis koji, saccharomyces cerevisiae
Koji, aspergillus niger koji, aspergillus oryzae koji are spare.
The production fermentation material of the ZL 201010148491.6 includes the following steps:
(1) lactobacillus acidophilus fermentation material is made:
A, lactobacillus acidophilus fermentation medium is made
Take the raw material of following weight: 15 parts~25 parts of corn flour, 40 parts~50 parts of wheat bran, bean cake powder 8 parts~20
Part, 1 part~3 parts of 8 parts~20 part, part yeast extract powder of corn germ cake, contains 70%~90% at 3 parts~8 parts of Cottonseed Meal powder
5 parts~15 parts of the corn pulp of moisture content, 5 parts~15 parts of molasses, 1 part of mountain flour~2 parts, calcium monohydrogen phosphate containing 40%~60% moisture content
1 part~2 parts；The raw material of acquirement is added into water, is stirred evenly, the weight ratio of the water of raw material and addition is 70~60: 30~40, is obtained
To compound material；Compound material is put into high pressure steam sterilization pin, is sterilized 30 minutes~50 points under 0.1Mpa~0.12Mpa air pressure
Clock；Then compound material is taken the dish out of the pot, material temperature degree to be composite is down to 25 DEG C~40 DEG C in an aseptic environment, obtains lactobacillus acidophilus culture
Base；
B, lactobacillus acidophilus is cultivated
Above-mentioned lactobacillus acidophilus kind liquid is added in lactobacillus acidophilus culture medium and is inoculated with, wherein the acidophilus being added
The weight ratio of lactobacillus species liquid and lactobacillus acidophilus culture medium is 1.5~2.5: 98.5~97.5, is 35 DEG C~42 in temperature
DEG C anaerobic environment under cultivate 6 days~8 days, to viable count be greater than 1X108It is a/gram when, then under 45 DEG C~50 DEG C low temperature environments
It is dry to water content≤12%, then sealed package, be kept in dark place, obtain lactobacillus acidophilus fermentation material；
(2) fermentation of bacillus subtilis material is made:
A, fermentation of bacillus subtilis culture medium is made
Take the raw material of following weight: 15 parts~25 parts of corn flour, 30 parts~50 parts of wheat bran, bean cake powder 5 parts~15
Part, 5 parts~15 parts of Cottonseed Meal powder, 10 parts~20 parts of corn germ cake, 2 parts~5 parts of the corn pulp containing 70%~90% moisture content,
2 parts~8 parts of the stake honey containing 40%~60% moisture content；The raw material of acquirement is added into water, is stirred evenly, the weight of the water of raw material and addition
The ratio between amount is 70~60: 30~40, obtains compound material；Compound material is put into high-pressure steam sterilizing pan, 0.1Mpa~
It sterilizes 30 minutes~50 minutes under 0.12Mpa air pressure；Then compound material is taken the dish out of the pot, material temperature degree to be composite is down in an aseptic environment
25 DEG C~40 DEG C, bacillus subtilis bacterium culture medium is made；
B, bacillus subtilis is cultivated
Above-mentioned Bacillus subtillis kind liquid is added in bacillus subtilis bacterium culture medium and is inoculated with, wherein be added
The weight ratio of bacillus subtilis strain liquid and bacillus subtilis bacterium culture medium is 1.5~2.5: 98.5~97.5, is in temperature
It is cultivated 2 days~3 days under 35 DEG C~42 DEG C of oxygen environment, is greater than 1X10 to bacillus subtilis viable count9It is a/gram, xylan
Enzymatic activity is greater than 1.2X104It when u/g, then dries to water content≤12% under 45 DEG C~50 DEG C low temperature environments, then sealing packet
It fills, be kept in dark place, obtain fermentation of bacillus subtilis material；
(3) fermentation by saccharomyces cerevisiae material is made:
A, fermentation by saccharomyces cerevisiae culture medium is made
Take the raw material of following weight: 10 parts~20 parts of corn flour, 20 parts~40 parts of wheat bran, bean cake powder 3 parts~8
Part, 5 parts~15 parts of wheat-middlings, contains 70%~90% moisture content at 8 parts~16 parts of Cottonseed Meal powder, 15 parts~25 parts of corn germ cake
2 parts~5 parts of corn pulp, 2 parts~8 parts of molasses containing 40%~60% moisture content；The raw material of acquirement is added into water, is stirred evenly, it is former
The weight ratio of material and the water being added is 70~60: 30~40, obtains compound material；Compound material is put into high-pressure steam sterilizing pan
In, it sterilizes 30 minutes~50 minutes under 0.1Mpa~0.12Mpa air pressure, obtains fermentation by saccharomyces cerevisiae culture medium；
B, saccharomyces cerevisiae is cultivated
Above-mentioned saccharomyces cerevisiae kind liquid is added in saccharomyces cerevisiae culture medium and is inoculated with, wherein the saccharomyces cerevisiae kind being added
The weight ratio of liquid and saccharomyces cerevisiae culture medium is 1.5~2.5: 98.5~97.5, the oxygen supply ring for being 35 DEG C~42 DEG C in temperature
It is cultivated 2 days~3 days under border, is greater than 1X10 to saccharomyces cerevisiae viable count9It is a/gram when, then under 45 DEG C~50 DEG C low temperature environments do
It is dry to water content≤12%, then sealed package, be kept in dark place, obtain fermentation by saccharomyces cerevisiae material；
(4) long handle trichoderma fermentation material is made:
A, the raw material of following weight: 5 parts~15 parts of corn flour, 30 parts~50 parts of wheat bran, bean cake powder 3 parts~8 is taken
Part, 10 parts~20 parts of Cottonseed Meal powder, 17 parts~27 parts of corn germ cake, the corn pulp 2 parts~5 containing 70%~90% moisture content
Part, 2 parts~8 parts of molasses containing 40%~60% moisture content；The raw material of acquirement is added into water, is stirred evenly, the water of raw material and addition
Weight ratio 65~55: 35~45, obtain compound material；Compound material is put into high-pressure steam sterilizing pan, 0.1Mpa~
It sterilizes 30 minutes~50 minutes under 0.12Mpa air pressure；Then compound material is taken the dish out of the pot, material temperature degree to be composite is down in an aseptic environment
25 DEG C~40 DEG C, long handle trichoderma fermentation medium is made；
B, long handle trichoderma is cultivated
Above-mentioned long handle trichoderma kind liquid is added in long handle trichoderma culture medium and is inoculated with, wherein the long handle trichoderma being added
The weight ratio of kind liquid and long handle trichoderma culture medium is 1.5~2.5: 98.5~97.5, the oxygen supply for being 35 DEG C~42 DEG C in temperature
It cultivates 2 days~3 days under environment, is dried extremely when cellulase activity is greater than 1200u/g, then under 45 DEG C~50 DEG C low temperature environments
Water content≤12%, then sealed package, be kept in dark place, obtain long handle trichoderma fermentation material；
(5) Zuo ?aspergillus fermentation material:
A, the raw material of following weight is taken: 10 parts~20 parts of corn flour, 30 parts~50 parts of wheat bran, 5 parts of bean cake powder~
15 parts, 5 parts~15 parts of Cottonseed Meal powder, 12 parts~22 parts of corn germ cake, the corn pulp 2 parts~5 containing 70%~90% moisture content
Part, 2 parts~8 parts of molasses containing 40%~60% moisture content；The raw material of acquirement is added into water, is stirred evenly, raw material and addition are made
The weight ratio of water is 65~55: 35~45, obtains compound material；Compound material is put into high-pressure steam sterilizing pan, in 0.1Mpa
It sterilizes 30~50 minutes under~0.12Mpa air pressure；Then compound material is taken the dish out of the pot, material temperature degree to be composite is down to 25 in an aseptic environment
DEG C~40 DEG C, fermentation of Aspergillus niger culture medium is made；
B, aspergillus niger is cultivated
Above-mentioned aspergillus niger kind liquid is added in aspergillus niger culture medium and is carried out by kind, wherein the aspergillus niger kind liquid being added with
The weight ratio of aspergillus niger culture medium is 1.5~2.5: 98.5~97.5, is trained in the case where temperature is 35 DEG C~42 DEG C of oxygen environment
It supports 2 days~3 days, dries when 1,4 beta-glucanase activity is greater than 10000u/g, then under 45 DEG C~50 DEG C low temperature environments to aqueous
Amount≤12%, then sealed package, be kept in dark place, obtain fermentation of Aspergillus niger material；
(6) aspergillus oryzae fermentation material is made:
A, the raw material of following weight is taken: 10 parts~20 parts of corn flour, 30 parts~50 parts of wheat bran, 5 parts of bean cake powder~
15 parts, 5 parts~15 parts of Cottonseed Meal powder, maize germ half from 12 parts~22 parts, 2 parts of the corn pulp containing 70%~90% moisture content~
5 parts, 2 parts~8 parts of molasses containing 40%~60% moisture content；The raw material of acquirement is added into water, is stirred evenly, raw material and addition are made
The weight ratio of water is 65~55: 35~45, obtains compound material；Compound material is put into high-pressure steam sterilizing pan, in 0.1Mpa
It sterilizes 30 minutes~50 minutes under~0.12Mpa air pressure；Then compound material is taken the dish out of the pot, in an aseptic environment material temperature degree drop to be composite
To 25 DEG C~40 DEG C, aspergillus oryzae fermentation medium is made；
B, aspergillus oryzae is cultivated
Above-mentioned aspergillus oryzae kind liquid is added in aspergillus oryzae culture medium and carries out by kind by one, wherein the aspergillus oryzae kind being added
The weight ratio of liquid and aspergillus oryzae culture medium is 1.5~2.5: 98.5~97.5, the oxygen environment for being 35 DEG C~42 DEG C in temperature
Lower culture 2 days~3 days is dried when proteinase activity is greater than 1500u/g, then under 45 DEG C~50 DEG C low temperature environments to water content
≤ 12%, then sealed package, be kept in dark place, obtain aspergillus oryzae fermentation material.
Two, check workshop temperature: keeping room temperature between 16 DEG C -30 DEG C.
Three, get the ammonium sulfate solution containing 3.0%-4.2% ready, between 16 DEG C -40 DEG C of solution temperature.
Four, are by two kinds or a kind of 15%-25% of lactobacillus acidophilus koji and saccharomyces cerevisiae koji, aspergillus niger koji and rice
Two kinds of aspergillus koji or a kind of 65%-45%, it is spare that bacillus subtilis koji 20%-30% is mixed into compound koji.
Whitewashing corn germ cake sample 2kg is in case contrasting detection before five, multiple spots take degradation.
Six, press the inoculum concentration of 3%-5%, and compound koji is accessed in whitewashing corn germ cake, after stirring 30min, adds and contains
The ammonium sulfate solution of 3.0%-4.2% to material moisture is 42%-48%, is sufficiently stirred.The material cloth stirred evenly
Enter fermentation tank, thickness of feed layer 80cm-120cm, (workshop is minimum need to keep room temperature at 16 DEG C or more), static fermentation 4h-
20h, to when temperature rises to 36 DEG C -38 DEG C, recycle my Patent No. ZL201410647232.6 institute public in bed of material 30cm
" the biofermentation slot turn over throw push-pull device at fixed " opened carries out turning over throwings (oxygenation) being cooled to 30 DEG C -27 DEG C, stops turning over throwing.Work as the bed of material
When temperature rises to 36 DEG C -38 DEG C again in 30cm, carries out turning over throwing (oxygenation) again and be cooled to 30 DEG C -27 DEG C, throwing is turned in stopping.Instead
Multiple operation, whole process keep 36h-48h.
Seven, turn over throwing cooling when material temperature no longer rapidly rises (fermentation process goes to stationary phase by logarithmic phase), by fermentation material
To 26 DEG C -28 DEG C, operation biofermentation slot turns over throwing push-pull device at fixed, material is smooth, compacting.Then 20h-30h, fermentation tank are sealed
In have sour taste spilling when, fermentative degradation termination.
Whitewashing corn germ cake sample 2kg is in case contrasting detection after eight, multiple spots take degradation
Nine, will degrade forward and backward whitewashing corn germ cake sample contrasting detection, record data.
Ten, are dried to moisture up to standard, are packed and stored.
Testing inspection data:
| Title | Butt vomitoxin (ppb) | Butt albumen (%) |
| Whitewashing corn germ cake | 3856 | 28 |
| Corn germ cake after fermentation | 1560 | 33.5 |
Conclusion: corn germ cake toxin decline 59.5%, detoxification efficiency is obvious.Appearance color is golden yellow, fragrant odour, is suitble to
Corn-soybean meal is partially substituted in animal and fowl fodder.
Claims (4)
1. utilizing " microbial fermentation antibiotic-free feed and preparation method thereof " of my Patent No. ZL 201010148491.6
In disclosed lactobacillus acidophilus fermentation material production method, fermentation of bacillus subtilis material production method, fermentation by saccharomyces cerevisiae material
Production method, fermentation of Aspergillus niger material production method, aspergillus oryzae fermentation material production method, and Patent No. ZL 2,012 2 in person
" self-supporting repeating transmission keyboard " disclosed in 0624896.7 makes lactobacillus acidophilus koji, bacillus subtilis koji, saccharomyces cerevisiae
Koji, aspergillus niger koji, aspergillus oryzae koji are spare.
2. by two kinds or a kind of 15%-25% of lactobacillus acidophilus koji and saccharomyces cerevisiae koji, aspergillus niger koji and aspergillus oryzae
Two kinds of koji or a kind of 65%-45%, it is spare that bacillus subtilis koji 20%-30% is mixed into compound koji.
3. pressing the inoculum concentration of 3%-5%, compound koji is accessed in whitewashing corn germ cake, after stirring 30min, is added containing 3.0%-
4.2% ammonium sulfate solution to material moisture is 42%-48%, is sufficiently stirred.The material stirred evenly is distributed into fermentation
Slot, thickness of feed layer 80cm-120cm, (workshop is minimum need to keep room temperature at 16 DEG C or more), static fermentation 4h-20h, wait expect
In layer 30cm, when temperature rises to 36 DEG C -38 DEG C, recycle disclosed in my Patent No. ZL 2,014 1 0647232.6
" biofermentation slot turn over throw push-pull device at fixed " carries out turning over throwings (oxygenation) being cooled to 30 DEG C -27 DEG C, stops turning over throwing.When in bed of material 30cm
When temperature rises to 36 DEG C -38 DEG C again, carries out turning over throwing (oxygenation) again and be cooled to 30 DEG C -27 DEG C, throwing is turned in stopping.It grasps repeatedly
Make, whole process keeps 36h-48h.
4. fermentation material is turned over throwing and is cooled to 26 when material temperature no longer rapidly rises (fermentation process goes to stationary phase by logarithmic phase)
DEG C -28 DEG C, operation biofermentation slot turns over throwing push-pull device at fixed, material is smooth, be compacted.Then 20h-30h is sealed, is had in fermentation tank
When sour taste overflows, fermentative degradation termination.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710904572 | 2017-09-29 | ||
| CN2017109045726 | 2017-09-29 |
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| CN109170512A true CN109170512A (en) | 2019-01-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201811099701.XA Pending CN109170512A (en) | 2017-09-29 | 2018-09-14 | The biodegradation method of vomitoxin in a kind of pair of corn germ cake |
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Cited By (1)
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| WO2023111835A1 (en) * | 2021-12-13 | 2023-06-22 | Living Technologies Cvba | Use of a composition for reducing and/or inactivating endotoxins |
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| WO2023111835A1 (en) * | 2021-12-13 | 2023-06-22 | Living Technologies Cvba | Use of a composition for reducing and/or inactivating endotoxins |
| BE1030009B1 (en) * | 2021-12-13 | 2023-07-10 | Living Tech Cvba | Use of a composition for reducing and/or inactivating endotoxins |
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